125 results on '"van Kleef, E"'
Search Results
2. From exposure to action : enhancing the effectiveness of front-of-pack nutrition labels
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van Trijp, J.C.M., van Kleef, E., Van Loo, E.J., Schruff-Lim, Eva-Maria, van Trijp, J.C.M., van Kleef, E., Van Loo, E.J., and Schruff-Lim, Eva-Maria
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- 2024
3. Superlijst Gezondheid 2024 : Welke supermarkt maakt van gezond de makkelijke keuze?
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Winkel, Deborah, de Jong, Dore, Haan, Gustaaf, van Engen-Cocquyt, Willem, Charles, Ambre, Hekman, Angela, Hekman, IJfana, van Kuppeveld, T., Linnebank, Charlotte, Simons, P.P.G., van Kleef, E., Roodenburg, Annet J.C., Seidell, Jaap C., Winkel, Deborah, de Jong, Dore, Haan, Gustaaf, van Engen-Cocquyt, Willem, Charles, Ambre, Hekman, Angela, Hekman, IJfana, van Kuppeveld, T., Linnebank, Charlotte, Simons, P.P.G., van Kleef, E., Roodenburg, Annet J.C., and Seidell, Jaap C.
- Abstract
Voor het eerst rapporteren alle supermarkten, behalve Plus, over de verkoop van producten in de Schijf van Vijf. Behalve Albert Heijn en Plus, hebben alle in Superlijst onderzochte supermarkten doelen gesteld om het aandeel Schijf van Vijf in hun verkoop te vergroten, wat een positieve ontwikkeling is richting een gezondere supermarkt. Deze ambities zijn echter nog niet terug te zien in de ontwikkeling van hun assortimenten, aanbiedingen en winkelinrichting, waarin ongezonde producten onverminderd de boventoon blijven voeren. Dat is schrijnend, omdat supermarkten in het Nationaal Preventieakkoord (2018) hebben beloofd maatregelen te nemen die consumenten helpen gezondere keuzes te maken, waaronder een jaarlijkse consumptiegroei van producten uit de Schijf van Vijf. In de ranglijst van 2024 loopt Ekoplaza voorop, gevolgd door middenmoters Lidl en Dirk. Jumbo, Aldi, Albert Heijn en Plus blijven achter.
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- 2024
4. Modelling studies of the transmission-dynamics and hospital burden of Clostridium difficile
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van Kleef, E., Edmunds, John, Robotham, J., Deeny, Sarah, and Jit, M.
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362.1969 - Abstract
Clostridium difficile, a Gram-positive spore-forming bacterium, is a source of considerable morbidity and mortality for patients treated in hospitals and other healthcare settings. Intestinal colonisation by C. difficile can cause infection (CDI) if the normal flora is disrupted, e.g. by the use of antimicrobials and some other drugs. Vaccines targeting C difficile main virulence factors, toxins A and B are currently undergoing clinical trials, however, their potential population impact is largely unknown. The work presented in this thesis aims to quantify the effectiveness of C. difficile vaccination in preventing hospitalonset CDI, including both its direct effects (reduction in individual patient morbidity and mortality) and indirect effects (prevention of onward transmission of the bacteria) using a mathematical dynamic transmission model framework. Based on a systematic literature review, it was shown that mathematical dynamic-transmission models have become an increasingly popular tool to help understand the patient-to-patient spread of nosocomial pathogens and predict the impact of healthcare prevention and control strategies. Methods have generally improved, with an increased use of stochastic models, and more advanced methods for formal model fitting and sensitivity analyses. Nonetheless, in contrast to methicillin-resistant Staphylococcus aureus – another bacterium commonly found in the healthcare setting – the transmission of C. difficile has rarely been considered within a dynamic modelling framework. Using national English CDI hospital surveillance data to fit a generalised additive mixed-effects model, this thesis revealed that, in line with recent evidence based on highly discriminatory genetic typingmethods, whilst transmission between symptomatic carriers was significant, this did not account for the majority of CDI cases in English hospitals. Asymptomatic carriers have been suggested as cocontributors, but their role in transmission remains uncertain to date. Previous estimates of additional excess bed days attributable to healthcare-acquired-CDI have varied widely, partly due to methodological weaknesses, and no robust estimates from a European setting are available. Both form key determinants to help quantify the health and economic burden of CDI, and are also likely to have an impact on the transmission-dynamics of the infection. Therefore, this thesis quantified the hospital burden of CDI, expressed in excess length of stay and mortality. A Cox proportional hazard model revealed that CDI was associated with a significantly decreased daily risk of discharge and increased risk of mortality, where the former was even further reduced for severe CDI patients. Using a multi-state model more intuitive estimates, i.e. the excess length of stay associated with mild (5 days [1.1-9.5]) and severe CDI (11.6 days [95% CI = 3.6-19.6]) were obtained. Finally, the results of an individual-based “state-of-the-art” dynamic transmission model in an English ICU (with epidemiological parameters informed by the findings of the statistical models mentioned, and with data-driven patient movement between the community, LTCF and ICU) showed that in settings with in-hospital acquisition rates comparable to the national average in English ICUs, immunising three patient groups: LTCF residents, elective patients and patients with a history of CDI in the ICU, resulted in a 43%, reduction of ICU-onset CDI. This required a relatively high number of vaccine doses, and a targeted strategy involving patients at high risk of colonisation on admission, such as LTCF residents proved more efficient. As these results proved highly sensitive to the level of antimicrobial use and in-ward acquisition rates, it was concluded that vaccination might be most efficient when targeting patient risk groups or settings where implementation of antimicrobial stewardship proves challenging.
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- 2015
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5. Seasonality of urinary tract infections in the United Kingdom in different age groups : longitudinal analysis of The Health Improvement Network (THIN)
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ROSELLO, A., POUWELS, K. B., DE CELLÈS, M. DOMENECH, VAN KLEEF, E., HAYWARD, A. C., HOPKINS, S., ROBOTHAM, J. V., SMIESZEK, T., OPATOWSKI, L., and DEENY, S. R.
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- 2018
6. Increased Expression of Glomerular von Willebrand Factor after Irradiation of the Mouse Kidney
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van Kleef, E. M., Oussoren, Y. G., Verheij, M., van de Pavert, I., Braunhut, S. J., Dewit, L. G. H., and Stewart, F. A.
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- 1998
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7. Dynamics of the Simplest Chlorine Atom Reaction: An Experimental and Theoretical Study
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Alagia, M., Balucani, N., Cartechini, L., Casavecchia, P., van Kleef, E. H., Volpi, G. G., Aoiz, F. J., Bañares, L., Schwenke, D. W., Allison, T. C., Mielke, S. L., and Truhlar, D. G.
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- 1996
8. Seasonal changes in the incidence of Escherichia coli bloodstream infection: variation with region and place of onset
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Deeny, S.R., van Kleef, E., Bou-Antoun, S., Hope, R.J., and Robotham, J.V.
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- 2015
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9. Superlijst Gezondheid 2022
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Winkel, D., Haan, Gustaaf, van Engen-Cocquyt, W., de Jong, D., Antens, A., Nijpjes, P., Baelde Jansen, D., Dagevos, H., van Kleef, E., Roodenburg, Annet J.C., Seidell, Jaap C., Winkel, D., Haan, Gustaaf, van Engen-Cocquyt, W., de Jong, D., Antens, A., Nijpjes, P., Baelde Jansen, D., Dagevos, H., van Kleef, E., Roodenburg, Annet J.C., and Seidell, Jaap C.
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- 2022
10. Adolescents’ exposure to and evaluation of food promotions on social media : a multi-method approach
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van der Bend, D.L.M., Jakstas, T., van Kleef, E., Shrewsbury, V.A., Bucher, T., van der Bend, D.L.M., Jakstas, T., van Kleef, E., Shrewsbury, V.A., and Bucher, T.
- Abstract
Background: Traditional food marketing, mostly involving advertisement of nutrient poor and energy dense foods, has the effect of enhancing attitudes, preferences, and increasing intake of marketed foods in adolescents, with detrimental consequences for health. While the use of social media applications in adolescents has proliferated, little is known about the content of food promotions within these applications. The aim of this study was to investigate adolescents’ exposure to and evaluation of social media food promotions (SMFPs). Methods: Australian adolescents aged 13–16 years joined one-on-one Zoom meetings with the researcher on the device they normally used for social media. Participants shared their screen and visited up to three of their favourite social media platforms for 10 min each, during which the researcher pointed out examples of SMFPs to participants. Next, participants answered questions about their awareness and appreciation of SMFPs. Screenshots of SMFPs were de-identified and analysed. Results: The study included 35 adolescents aged 14.4 (± 1.2) years (boys: n = 18; girls: n = 17). Instagram, Snapchat and YouTube were the most favoured social media platforms. During a total of 1000 min of viewing time, 1801 unbranded (n = 1221) and branded (n = 580) SMFPs were identified. Participants viewed a median rate (IQR) of 12.0 (6.3–20) SMFPs per 10 min, with a median rate of 6.0 (3–11) non-core SMFPs per 10 min. A majority of SMFPs (62%) were embedded into celebrity influencer or entertaining content (e.g., vlogs, cooking videos, streamed TV content). In total, 60% of the participants said they had sometimes, rarely or never noticed the SMFPs pointed out by the researcher themselves. Participants largely remembered non-core foods or brands (77%). Almost half (49%) of participants liked SMFPs, while only 6% disliked them. Conclusions: This study contributes to a relatively unexplored research area. The outcomes show adolescents’ SMFP exposure mostly co
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- 2022
11. Dutch consumers’ perception of sustainable packaging for milk products, a qualitative and quantitative study
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Liem, D.G., in 't Groen, A., van Kleef, E., Liem, D.G., in 't Groen, A., and van Kleef, E.
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Visual packaging cues might assist consumers making sustainable choices. In a qualitative study potential packaging cues were explored (study 1). Those cues mostly related to sustainability were tested in a quantitative follow up study (study 2). Study 1: Consumers (n = 30, 39.4 ± 17.1 yrs.) were divided in four focus group discussions in which participants completed a free association task and sorting task with 10 milk package designs which differed in colour, material and shape. Next, participants followed a semi structured focus group discussion in which they could elaborate on their answers. Results revealed 7 major themes: 1) nature of material, 2) expected visible harm to environment, 3) possibility to reuse and recycle packaging, 4) unnecessary packaging, 5) appearance of packaging (colour & feel), 6) incongruency of packaging with content, and 7) consumer and industry responsibility. Colour and material generated the most discussion around sustainability and were subsequently investigated in study 2. Study 2: Consumers (n = 104, 26 ± 9.1 yrs.) were invited to an online survey during which they were presented with 4 pack designs which were manipulated in colour (i.e., white, vs cardboard brown) and materials (i.e., smooth vs rough). Cardboard brown and rough pack designs were perceived as significantly more sustainable than white pack designs (F(1,103) = 42.72, p < 0.001) and smooth pack designs (F(1,103) = 97.68, p < 0.001). No interaction was observed between material and colour in the impact of perceived sustainability. Consumers’ perception of sustainability is mostly driven by their belief whether a packaging is recyclable and/or reusable. Visible carboard texture in combination with a carboard look increases consumers’ perception of sustainability.
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- 2022
12. Additional file 1 of Adolescents’ exposure to and evaluation of food promotions on social media: a multi-method approach
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van der Bend, D. L. M., Jakstas, T, van Kleef, E, Shrewsbury, V. A., and Bucher, T
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Additional file 1: Table 1a Pre-study questions on participants’ socio-demographic characteristics and social media use. Table 1b List of most popular social media platforms in Australia (July 2020) and answer categories on how often they are used in the past month. Table 2 Post-study questions on recognition and appreciation of social media food promotions. Table 3 Coding of composite food or beverages on social media into core and non-core classifications. Table 4 Participants’ duration of use, device used to access, and main reason for using their favourite social media platforms.
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- 2022
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13. Factors influencing SARS-CoV-2 infection rate in Belgian nursing home residents during the first wave of COVID-19 pandemic
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Peckeu-Abboud, L., primary, van Kleef, E., additional, Smekens, T., additional, Latour, K., additional, Dequeker, S., additional, Int Panis, L., additional, and Laga, M., additional
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- 2022
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14. CONGENITAL MUSCULAR DYSTROPHIES
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Bouman, K., primary, Groothuis, J., additional, Doorduin, J., additional, van Alfen, N., additional, ten Cate, F. Udink, additional, van den Heuvel, F., additional, Nijveldt, R., additional, van Tilburg, W., additional, Buckens, S., additional, Dittrich, A., additional, Draaisma, J., additional, Janssen, M., additional, Kamsteeg, E., additional, van Kleef, E., additional, Smeitink, J., additional, van Tienen, F., additional, Smeets, H., additional, van Engelen, B., additional, Erasmus, C., additional, and Voermans, N., additional
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- 2021
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15. Impact of the COVID-19 pandemic and response on the utilisation of health services during the first wave in Kinshasa, the Democratic Republic of the Congo
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Karen A. Grépin, Katanga En, Celestin Hategeka, Grégoire Lurton, Vanlerberghe, Faustin Chenge, van Kleef E, Simone E Carter, Mwamba Dk, and Serge Ma-Nitu Mayaka
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medicine.medical_specialty ,business.industry ,Public health ,Social distance ,Outbreak ,medicine.disease ,Health informatics ,symbols.namesake ,Health facility ,Environmental health ,Pandemic ,medicine ,symbols ,Poisson regression ,business ,Malaria - Abstract
IntroductionHealth service use among the general public can decline during infectious disease outbreaks and has been predicted among low and middle-income countries during the COVID-19 pandemic. In March 2020, the government of the Democratic Republic of the Congo (DRC) implemented public health measures across Kinshasa, including strict lockdown measures in the Gombe health zone, to mitigate impact of the pandemic.MethodsUsing data from the Health Management Information System (January 2018 - December 2020), we evaluated the impact of the pandemic on the use of essential health services (total visits, maternal health, vaccinations, visits for common infectious diseases, and diagnosis of non-communicable diseases) using interrupted time series with mixed effects segmented Poisson regression models during the first wave of the pandemic. Analyses were stratified by age, sex, health facility, and neighbourhood.ResultsHealth service use dropped rapidly following the start of the pandemic and ranged from 16% for hypertension diagnoses to 39% for diabetes diagnoses. However, reductions were highly concentrated in Gombe (81% decline in total visits) relative to health zones without lockdown. When the lockdown was lifted, total visits, visits for infectious diseases, and diagnoses for non-communicable diseases increased approximately two-fold. Hospitals were more affected than health centres. Overall, the use of maternal health services and vaccinations was not significantly affected.ConclusionThe COVID-19 pandemic resulted in important reductions in health service utilisation in Kinshasa, particularly Gombe. Lifting of lockdown led to a rebound in the level of health service use but it remained lower than pre-pandemic levels.Summary BoxWhat is already known about this subjectSubstantial declines in the use of health services among the general public have been well-documented during previous outbreaks of infectious diseases.Modelled studies predicted substantial increases in morbidity and mortality in many low- and middle-income countries (LMICs) mainly due to expected declines in the use of health services among the general public.Only a small number of studies have so far evaluated the impact of the COVID-19 pandemic on the use of health services in LMICs and none have also evaluated both the implementation and lifting of lockdown measures.What are the new findingsThis study found that overall use of health services declined in Kinshasa but was most pronounced in the Gombe health zone which was subject to strict lockdown measures.Some health services were more affected than others, most notably visits and tests for malaria and visits for new diagnoses of non-communicable diseases. Maternal and child health services were relatively unaffected.When the lockdown measures were lifted, health service utilization rebounded but remained at levels lower than those observed pre-pandemic.What do the new findings implyThe COVID-19 pandemic has likely had important effects on the use of health services among the general public throughout LMICs. However, evidence from Kinshasa suggests the effects may not be as widespread as previously assumed.The impact of strict social distancing measures needs on COVID-19 outcomes needs to be weighed off against the potential population-level health effects of these policies in various international contexts.
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- 2021
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16. Internally Regulated Eating Style: A Comprehensive Theoretical Framework
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Palascha, A., van Kleef, E., de Vet, E.W.M.L., van Trijp, J.C.M., Palascha, A., van Kleef, E., de Vet, E.W.M.L., and van Trijp, J.C.M.
- Abstract
Internally regulated eating style, the eating style that is driven by internal bodily sensations of hunger and satiation, is a concept that has received increasing attention in the literature and health practice over the last decades. The various attempts that have been made so far to conceptualize internally regulated eating have taken place independently of one another and each sheds light on only parts of the total picture of what defines internally regulated eating. This has resulted in a literature that is rather fragmented. More importantly, it is not yet clear which are the characteristics that comprise this eating style. In this paper, we identify and describe the full spectrum of these characteristics, namely, sensitivity to internal hunger and satiation signals, self-efficacy in using internal hunger and satiation signals, self-trusting attitude for the regulation of eating, relaxed relationship with food, and tendency to savor the food while eating. With this research, we introduce a common language to the field and we present a new theoretical framework that does justice not just to the full breadth of characteristics that are necessary for the internally regulated eating style but also to the associations between them and the potential mechanisms by which they contribute to this eating style.
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- 2021
17. Gezonder op de basisschool: schoollunches en meer bewegen : Een verkenning naar draagvlak, haalbaarheid, betaalbaarheid en impact
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van Giessen, A, Oosterhoff, M, Hoekstra, J, Over, EAB, Joore, MA, van Schayck, OCP, Willeboordse, M, Dijkstra, SC, Seidell, JC, van Kleef, E, Soethoudt, H, and Vingerhoeds, MA
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Marketing and Consumer Behaviour ,Health & Consumer Research ,RIVM rapport 2020-0161 ,Food ,Supply Chain & Information Management ,Life Science ,WASS ,Marktkunde en Consumentengedrag ,Food, Health & Consumer Research ,VLAG - Abstract
Een verkenning naar draagvlak, haalbaarheid, betaalbaarheid en impact De overheid stimuleert dat basisscholen kinderen helpen om gezond te leven. Een gezonde schoollunch en meer bewegen onder schooltijd kunnen hierbij helpen. Uit onderzoek blijkt dat hier draagvlak voor is onder ouders, kinderen en scholen. Ook is het haalbaar, mits al deze partijen worden betrokken bij de organisatie. Ouders zijn bereid om mee te betalen (1,75 euro per kind per dag), maar daarmee zijn de kosten (2,40 euro per kind per dag voor de zelfsmeerlunch en 4,50 euro voor de schoollunch met beweegactiviteiten) niet gedekt. Ondersteuning is dus nodig om minder draagkrachtige gezinnen financieel te steunen. Bij een zelfsmeerlunch maken kinderen zelf op school een gezonde lunch klaar. Ze eten dan meer fruit, groente en bruinbrood en drinken meer melk en water. Voor een zelfsmeerlunch is minimaal 30 minuten tijd nodig. Een cateraar kan de benodigdheden inkopen en leveren. De school kan dit ook zelf doen, maar dat vraagt om een goede coördinatie. Extra beweegmomenten zijn mogelijk door tijdens de lessen korte momenten daarvoor in te lassen. Pedagogisch medewerkers, zoals van de naschoolse opvang, en buurtsportcoaches van gemeenten, kunnen helpen bij sport- en spelactiviteiten. Door beweegactiviteiten en de zelfsmeerlunch hebben kinderen een gezonder gewicht. Het percentage kinderen van 4 tot 18 jaar met overgewicht zou kunnen dalen van 13,5 procent naar 10,2 procent in 2040, en met obesitas van 2,8 procent naar 2,3 procent. In de toekomst kan de daling eraan bijdragen dat minder volwassenen overgewicht, obesitas en diabetes hebben. Dan moeten de kinderen het veranderde gedrag wel blijven volhouden als ze volwassen zijn. Om kinderen hierbij te helpen kunnen schoollunches en beweegactiviteiten worden aangevuld met activiteiten op de middelbare school en in de wijk. Aanleiding voor deze verkenning zijn eerdere succesvolle projecten met gezonde schoollunches en meer bewegen in het basisonderwijs.
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- 2020
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18. Gezonder op de basisschool: schoollunches en meer bewegen : een verkenning naar draagvlak, haalbaarheid, betaalbaarheid en impact
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van Giessen, A., Oosterhoff, M., Jacobs, E.A.B., van Schayk, O.C.P., Willeboordse, M., Dijkstra, S.C., Seidell, J.C., van Kleef, E., Soethoudt, H., Vingerhoeds, M.H., van Giessen, A., Oosterhoff, M., Jacobs, E.A.B., van Schayk, O.C.P., Willeboordse, M., Dijkstra, S.C., Seidell, J.C., van Kleef, E., Soethoudt, H., and Vingerhoeds, M.H.
- Abstract
Een verkenning naar draagvlak, haalbaarheid, betaalbaarheid en impact De overheid stimuleert dat basisscholen kinderen helpen om gezond te leven. Een gezonde schoollunch en meer bewegen onder schooltijd kunnen hierbij helpen. Uit onderzoek blijkt dat hier draagvlak voor is onder ouders, kinderen en scholen. Ook is het haalbaar, mits al deze partijen worden betrokken bij de organisatie. Ouders zijn bereid om mee te betalen (1,75 euro per kind per dag), maar daarmee zijn de kosten (2,40 euro per kind per dag voor de zelfsmeerlunch en 4,50 euro voor de schoollunch met beweegactiviteiten) niet gedekt. Ondersteuning is dus nodig om minder draagkrachtige gezinnen financieel te steunen. Bij een zelfsmeerlunch maken kinderen zelf op school een gezonde lunch klaar. Ze eten dan meer fruit, groente en bruinbrood en drinken meer melk en water. Voor een zelfsmeerlunch is minimaal 30 minuten tijd nodig. Een cateraar kan de benodigdheden inkopen en leveren. De school kan dit ook zelf doen, maar dat vraagt om een goede coördinatie. Extra beweegmomenten zijn mogelijk door tijdens de lessen korte momenten daarvoor in te lassen. Pedagogisch medewerkers, zoals van de naschoolse opvang, en buurtsportcoaches van gemeenten, kunnen helpen bij sport- en spelactiviteiten. Door beweegactiviteiten en de zelfsmeerlunch hebben kinderen een gezonder gewicht. Het percentage kinderen van 4 tot 18 jaar met overgewicht zou kunnen dalen van 13,5 procent naar 10,2 procent in 2040, en met obesitas van 2,8 procent naar 2,3 procent. In de toekomst kan de daling eraan bijdragen dat minder volwassenen overgewicht, obesitas en diabetes hebben. Dan moeten de kinderen het veranderde gedrag wel blijven volhouden als ze volwassen zijn. Om kinderen hierbij te helpen kunnen schoollunches en beweegactiviteiten worden aangevuld met activiteiten op de middelbare school en in de wijk. Aanleiding voor deze verkenning zijn eerdere succesvolle projecten met gezonde schoollunches en meer bewegen in het basisonderwijs.
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- 2020
19. KBTBD13 is an actin-binding protein that modulates muscle kinetics
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de Winter, J M, Molenaar, J P, Yuen, M, van der Pijl, R, Shen, S, Conijn, S, van de Locht, M, Willigenburg, M, Bogaards, S J P, van Kleef, E S B, Lassche, S, Persson, Malin, Rassier, D E, Sztal, T E, Ruparelia, A A, Oorschot, V, Ramm, G, Hall, T E, Xiong, Z, Johnson, C N, Li, F, Kiss, B, Lozano-Vidal, N, Boon, R A, Marabita, M, Nogara, L, Blaauw, B, Rodenburg, R J, Kusters, B, Doorduin, J, Beggs, A H, Granzier, H, Campbell, K, Ma, W, Irving, T, Malfatti, E, Romero, N B, Bryson-Richardson, R J, van Engelen, B G M, Voermans, N C, Ottenheijm, C A C, de Winter, J M, Molenaar, J P, Yuen, M, van der Pijl, R, Shen, S, Conijn, S, van de Locht, M, Willigenburg, M, Bogaards, S J P, van Kleef, E S B, Lassche, S, Persson, Malin, Rassier, D E, Sztal, T E, Ruparelia, A A, Oorschot, V, Ramm, G, Hall, T E, Xiong, Z, Johnson, C N, Li, F, Kiss, B, Lozano-Vidal, N, Boon, R A, Marabita, M, Nogara, L, Blaauw, B, Rodenburg, R J, Kusters, B, Doorduin, J, Beggs, A H, Granzier, H, Campbell, K, Ma, W, Irving, T, Malfatti, E, Romero, N B, Bryson-Richardson, R J, van Engelen, B G M, Voermans, N C, and Ottenheijm, C A C
- Abstract
The mechanisms that modulate the kinetics of muscle relaxation are critically important for muscle function. A prime example of the impact of impaired relaxation kinetics is nemaline myopathy caused by mutations in KBTBD13 (NEM6). In addition to weakness, NEM6 patients have slow muscle relaxation, compromising contractility and daily life activities. The role of KBTBD13 in muscle is unknown, and the pathomechanism underlying NEM6 is undetermined. A combination of transcranial magnetic stimulation-induced muscle relaxation, muscle fiber- and sarcomere-contractility assays, low-angle x-ray diffraction, and superresolution microscopy revealed that the impaired muscle-relaxation kinetics in NEM6 patients are caused by structural changes in the thin filament, a sarcomeric microstructure. Using homology modeling and binding and contractility assays with recombinant KBTBD13, Kbtbd13-knockout and Kbtbd13(R408c)-knockin mouse models, and a GFP-labeled Kbtbd13-transgenic zebrafish model, we discovered that KBTBD13 binds to actin - a major constituent of the thin filament - and that mutations in KBTBD13 cause structural changes impairing muscle-relaxation kinetics. We propose that this actin-based impaired relaxation is central to NEM6 pathology., Funding agencies:Dutch Foundation for Scientific Research VIDI 016.126.319Princess Beatrix Muscle Foundation W.OR17-08H2020-MSCA-RISE-2014 645648Advanced Photon Source DE-AC02-06CH11357Foundation Building Strength for Nemaline MyopathyNational Health and Medical Research Council (NHMRC) of Australia APP1121651 United States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) NIH R01 HD075802 Muscular Dystrophy Association MDA602235 NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) NIH R01 AR053897 United States Department of Health & Human Services National Institutes of Health (NIH) - USA HL133359 United States Department of Energy (DOE) DE-AC02-06CH11357 NIH National Institute of General Medical Sciences (NIGMS)9 P41 GM103622 1S10OD018090-01
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- 2020
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20. Development and validation of the Multidimensional Internally Regulated Eating Scale (MIRES)
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Palascha, A., van Kleef, E., de Vet, E.W.M.L., van Trijp, J.C.M., Palascha, A., van Kleef, E., de Vet, E.W.M.L., and van Trijp, J.C.M.
- Abstract
In this paper, we describe the systematic development and validation of the Multidimensional Internally Regulated Eating Scale (MIRES), a new self-report instrument that quantifies the individual-difference characteristics that together shape the inclination towards eating in response to internal bodily sensations of hunger and satiation (i.e., internally regulated eating style). MIRES is a 21-item scale consisting of seven subscales, which have high internal consistency and adequate to high two-week temporal stability. The MIRES model, as tested in community samples from the UK and US, had a very good fit to the data both at the level of individual subscales, but also as a higher-order formative model. High and significant correlations with measures of intuitive eating and eating competence lent support to the convergent validity of MIRES, while its incremental validity in relation to these measures was also upheld. MIRES as a formative construct, as well as all individual subscales, correlated negatively with eating disorder symptomatology and weight-related measures (e.g., BMI, weight cycling) and positively with adaptive behavioral and psychological outcomes (e.g., proactive coping, body appreciation, life satisfaction), supporting the criterion validity of the scale. This endeavor has resulted in a reliable and valid instrument to be used for the thorough assessment of the features that synthesize the profile of those who tend to regulate their eating internally.
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- 2020
21. Puntprevalentieonderzoek naar antibioticaresistentie in verpleeghuizen
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van Kleef, E, Wielders, L, Bijkerk, P, Beishuizen, B, Schouls, L, van der Lubben, M, de Greeff, S, van Kleef, E, Wielders, L, Bijkerk, P, Beishuizen, B, Schouls, L, van der Lubben, M, and de Greeff, S
- Abstract
RIVM rapport:In 2018 is in Nederland een landelijk onderzoek gestart naar dragerschap van antibioticaresistente bacteriën onder verpleeghuisbewoners. Als geheel staat de verpleeghuissector er goed voor. Door dit onderzoek is er een completer beeld van antibioticaresistentie in Nederland. Voor dit onderzoek zijn 4420 bewoners in 159 verpleeghuizen onderzocht. Er is naar twee soorten resistente darmbacteriën onderzoek gedaan (ESBL en CPE). Er werd geen CPE aangetroffen. Dat is gunstig, want er zijn nauwelijks antibiotica die infecties met deze bacterie kunnen behandelen. Het aandeel bewoners van verpleeghuizen dat een ESBL-bacterie bij zich droeg komt gemiddeld genomen overeen met het percentage in de Nederlandse bevolking. ESBL's zijn enzymen die veelgebruikte antibiotica zoals penicillines kunnen afbreken waardoor de antibiotica niet meer werken. Bij een derde van de deelnemende verpleeghuizen, kwamen meer ESBL-bacteriën onder bewoners voor dan gemiddeld in Nederland. Bij hen is extra onderzoek gedaan om te bepalen of er sprake was van verspreiding onder de bewoners. Ook kregen ze advies over maatregelen om ervoor te zorgen dat de bacteriën zich niet verder verspreiden. Het onderzoek is onderdeel van de landelijke aanpak van antibioticaresistentie door de Nederlandse overheid. Het doel is om verdere resistentie te voorkomen en de gevolgen ervan zo veel mogelijk te beperken. Tot dit onderzoek werd uitgevoerd was er nog weinig zicht op de situatie in verpleeghuizen. De landelijke aanpak is opgezet omdat bacteriën wereldwijd steeds vaker ongevoelig worden voor antibiotica. Het RIVM coördineerde de studie, die is uitgevoerd door Regionale Zorgnetwerken Antibioticaresistentie (RZN), medisch microbiologische laboratoria, en verpleeghuizen. De RZN zijn opgezet om antibioticaresistentie regionaal te voorkomen en te bestrijden., In 2018, the Dutch Ministry of Health, Welfare and Sport commissioned a nationwide study investigating the presence of antibiotic resistant bacteria among nursing home residents. As a whole, the study revealed that nursing home sector is in good shape. Nonetheless, attention to proper infection prevention and hygiene measures is still needed. This study has improved our understanding of antibiotic resistance in the Netherlands. For the study, 4420 residents of 159 nursing homes were examined, looking for two types of resistant gut bacteria: ESBL and CPE. No CPE was found. This is positive, as there are hardly any antibiotic treatment options for infections caused by this bacterium. The fraction of nursing home residents with ESBL bacteria did not differ from the average percentage of ESBL carriers in the Dutch population. ESBLs are enzymes that can break down commonly used antibiotics such as penicillins, resulting in the antibiotics no longer being effective. In a third of the participating nursing homes, ESBL bacteria occurred more often among residents than on average amongst the general population of the Netherlands. In these nursing homes, extra investigations were carried out to see if the bacteria had spread among the residents. They were also given advice on infection prevention control measures to prevent further spread. The study is part of the Dutch national approach for tackling antibiotic resistance. The aim is to prevent further resistance and to reduce its consequences as much as possible. The situation in nursing homes was rather unclear prior to starting this study. The national approach was set up because bacteria throughout the world are becoming insensitive to antibiotics increasingly often. RIVM, the National Institute for Public Health and the Environment, coordinated the study and carried out the study together with the Regional Care Networks for Antibiotic Resistance (RZN), medical microbiology laboratories and nursing homes. The RZNs have be
- Published
- 2019
22. What’s for lunch? The content and quality of lunches consumed by Dutch primary schoolchildren and the differences between lunches consumed at home and at school
- Author
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Rongen, Frédérique C., van Kleef, E., Sanjaya, S., Vingerhoeds, M.H., Buurma-Rethans, E., van den Bogaard, C., van Rossum, Caroline, Seidell, Jacob C., Dijkstra, Coosje, Rongen, Frédérique C., van Kleef, E., Sanjaya, S., Vingerhoeds, M.H., Buurma-Rethans, E., van den Bogaard, C., van Rossum, Caroline, Seidell, Jacob C., and Dijkstra, Coosje
- Abstract
Background Lunch is an important part of a healthy diet, which is essential for the development, growth and academic performance of school-aged children. Currently there is an increasing number of Dutch primary schoolchildren who are transitioning from eating lunch at home to school. There is limited knowledge about the current quality of the lunches consumed by primary schoolchildren in the Netherlands and whether there are any differences between lunches consumed at home or at school. To investigate differences in content and quality of lunches consumed by Dutch primary schoolchildren at home and at school. Methods Cross-sectional study among 363 Dutch primary schoolchildren aged 4–12 years based on the first two years of the 2012–2016 Dutch National Food Consumption Survey. Demographic characteristics were obtained through a questionnaire. Diet was assessed with two non-consecutive 24-h dietary recalls. Quality of lunches was assessed on their nutritional quality whether they fitted the nutritional guidelines. ‘Nonparametric tests were used to examine the content and quality of the lunches between place of consumption and parental educational position. Results The most consumed lunch products among primary schoolchildren were bread, dairy products and sugar-sweetened beverages. Fruit and vegetable consumption was very low. Consumption of milk and other dairy products was higher among children who eat lunch at home than children who eat lunch at school (p < 0.01). Consumption of sugar-sweetened beverages was higher among children who eat lunch at school than children who eat lunch at home (p < 0.01), and at school a higher proportion of the drinks did not fit within the Dutch dietary recommendations (p < 0.01). Conclusions The current content of the lunches consumed by Dutch primary schoolchildren leaves room for improvement, especially regarding fruit and vegetables. The statistically significantly higher consumption of sugar-sweetened beverages and lower consump
- Published
- 2019
23. CONGENITAL MUSCULAR DYSTROPHIES: EP.69 LAST STRONG: LAMA2 and SELENON to study trial readiness, outcome measures and natural history
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Bouman, K., Groothuis, J., Doorduin, J., van Alfen, N., ten Cate, F. Udink, van den Heuvel, F., Nijveldt, R., van Tilburg, W., Buckens, S., Dittrich, A., Draaisma, J., Janssen, M., Kamsteeg, E., van Kleef, E., Smeitink, J., van Tienen, F., Smeets, H., van Engelen, B., Erasmus, C., and Voermans, N.
- Published
- 2021
- Full Text
- View/download PDF
24. Is nudgen naar volkorenbrood mogelijk? : Nederlanders moeten meer vezels eten
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Vingerhoeds, M.H., Koning, Rosella, and van Kleef, E.
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Life Science - Abstract
De consumptie van voldoende vezels is een belangrijke factor inhet voorkomen van welvaartsaandoeningen, zoals obesitas, diabetesII, hart- en vaatziekten en bepaalde vormen van kanker.Per dag wordt geadviseerd 30 tot 40 gram vezels te eten. De huidigeinname is gemiddeld echter slechts 18-23 gram per dag (1,2).Het lijkt daarom een goede zaak de consumptie van bijvoorbeeldvolkorenbrood te stimuleren. Nudging blijkt echter niet steeds degewenste resultaten te hebben.
- Published
- 2017
25. Seasonality of urinary tract infections in the United Kingdom in different age groups: longitudinal analysis of The Health Improvement Network (THIN)
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ROSELLO, A., primary, POUWELS, K. B., additional, DOMENECH DE CELLÈS, M., additional, VAN KLEEF, E., additional, HAYWARD, A. C., additional, HOPKINS, S., additional, ROBOTHAM, J. V., additional, SMIESZEK, T., additional, OPATOWSKI, L., additional, and DEENY, S. R., additional
- Published
- 2017
- Full Text
- View/download PDF
26. Commanding to 'Nudge' via the Proportionality Principle?
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Purnhagen, K.P. (Kai), van Kleef, E. (Ellen), Purnhagen, K.P. (Kai), and van Kleef, E. (Ellen)
- Abstract
This piece assesses whether nudging techniques can be argued to be a less restrictive but equally effective way to regulate diets in EU law. It has been argued that nudging techniques, due to their freedom-preserving nature, might influence the proportionality test in such a way that authorities need to give preference to nudging techniques over content-related or information regulation. We will illustrate on the example of EU food law how behavioural sciences have first altered the EU food law’s goal from the mere provision of safety to also steering behaviour towards healthier diets. In line with this development, the regulatory toolbox advanced beyond the traditional dichotomy of content-related vs. information-related regulation, eventually adding nudging as a third way to regulate. Drawing on previous works of legal scholars we will then present the hypothesis that nudging techniques, according to their choice preserving nature, may be less restrictive but equally effective when contrasted to information-related over content-related regulation. With reference to recent CJEU case law that such a claim needs to be backed up by scientific evidence, we will evaluate several nudging studies in the area of food that test the effectiveness of this approach. We will illustrate that, while nudging indeed has a choice-preserving nature and therefore might be less restrictive, it may also be classified under certain circumstances equally effective to information-related regulation. From the evidence presented the proportionality principle may in some cases require EU policy makers to primarily use nudges instead of information-related regulation.
- Published
- 2017
27. Excess length of stay and mortality due to Clostridium difficile infection: a multi-state modelling approach
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van Kleef, E, Green, N, Goldenberg, SD, Robotham, JV, Cookson, B, Jit, M, Edmunds, WJ, and Deeny, SR
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genetic structures - Abstract
BACKGROUND: The burden of healthcare-associated infections, such as healthcare-acquired Clostridium difficile (HA-CDI), can be expressed in terms of additional length of stay (LOS) and mortality. However, previous estimates have varied widely. Although some have considered time of infection onset (time-dependent bias), none considered the impact of severity of HA-CDI; this was the primary aim of this study. METHODS: The daily risk of in-hospital death or discharge was modelled using a Cox proportional hazards model, fitted to data on patients discharged in 2012 from a large English teaching hospital. We treated HA-CDI status as a time-dependent variable and adjusted for confounders. In addition, a multi-state model was developed to provide a clinically intuitive metric of delayed discharge associated with non-severe and severe HA-CDI respectively. FINDINGS: Data comprised 157 (including 48 severe) HA-CDI cases among 42,618 patients. HA-CDI reduced the daily discharge rate by nearly one-quarter [hazard ratio (HR): 0.72; 95% confidence interval (CI): 0.61-0.84] and increased the in-hospital death rate by 75% compared with non-HA-CDI patients (HR: 1.75; 95% CI: 1.16-2.62). Whereas overall HA-CDI resulted in a mean excess LOS of about seven days (95% CI: 3.5-10.9), severe cases had an average excess LOS which was twice (∼11.6 days; 95% CI: 3.6-19.6) that of the non-severe cases (about five days; 95% CI: 1.1-9.5). CONCLUSION: HA-CDI contributes to patients' expected LOS and risk of mortality. However, when quantifying the health and economic burden of hospital-onset of HA-CDI, the heterogeneity in the impact of HA-CDI should be accounted for.
- Published
- 2014
28. An In-Store Experiment on the Effect of Accessibility on Sales of Wholegrain and White Bread in Supermarkets
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de Wijk, R.A., Maaskant, A.J., Polet, I.A., Holthuysen, N.T.E., van Kleef, E., Vingerhoeds, M.H., de Wijk, R.A., Maaskant, A.J., Polet, I.A., Holthuysen, N.T.E., van Kleef, E., and Vingerhoeds, M.H.
- Abstract
Even though whole grain foods have various health benefits, consumers have been found not to eat enough of them. Nudging interventions are built on the premise that food purchases and consumption are strongly influenced by the environment in which decisions are made. Little research has been conducted to examine the influence of a small and inexpensive nudging intervention on bread choices in a real-life supermarket context. An in-store experiment was conducted in two six-week periods in two supermarkets to investigate theeffects of accessibility on consumers’ purchase of healthier whole grain and other types of bread. In the high accessibility condition, healthier bread was placed in a more convenient location for the shopper on the left side of the shelves where it was encountered first. In the low accessibility condition, it was placed on the right side. There were consistent significant differences in sales between supermarkets, types of bread, day of the week, but not between low and high accessibility. Additional research is needed to better understand theeffects of convenience and accessibility on bread choices.
- Published
- 2016
29. Dinner Rituals That Correlate with Child and Adult BMI
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Wansink, B. and van Kleef, E.
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Marketing and Consumer Behaviour ,digestive, oral, and skin physiology ,WASS ,family meals ,diet quality ,food-intake ,eating behavior ,television ,WU Omgevingswetenschappen Marketing Communicatie ,illusions ,patterns ,Marktkunde en Consumentengedrag ,adolescents ,program - Abstract
Objective: What predicts whether a child will be at risk for obesity? Whereas past research has focused on foods, eating habits, feeding styles, and family meal patterns, this study departs from a food-centric approach to examine how various dinner rituals might influence the BMIs of children and adults. Methods: In this study of 190 parents (BMI529.167.2) and 148 children (BMI520.364.4), the relationship between their BMIs and everyday family dinner rituals was examined using both correlation and regression analysis (controlled for educational level of parents). Results: Families who frequently ate dinner in the kitchen or dining room had significantly lower BMIs for both adults (r520.31) and children (r520.24) compared to families who ate elsewhere. Additionally, helping cook dinner was associated with higher BMI for girls (r50.26), and remaining at the table until everyone is finished with eating was associated with lower BMI for boys (r520.31). Conclusions: Dinner tables may be one place where social support and family involvement meet—both of which relate to the BMI of children as well as parents. Family meals and their rituals might be an underappreciated battleground to fight obesity.
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- 2014
30. Evaluation of Segmentation Bases for the Heterogeneous Elderly Consumer Population: the Functional Food Market
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van der Zanden, L.D.T., van Kleef, E., de Wijk, R.A., and van Trijp, J.C.M.
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Marketing and Consumer Behaviour ,benefit segmentation ,attitudes ,WASS ,health ,mature consumers ,patronage motives ,cognitive age ,future time perspective ,willingness ,Marktkunde en Consumentengedrag ,Consumer Science & Intelligent Systems ,needs-based segmentation ,older consumers - Abstract
It is beneficial for both the public health community and the food industry to meet nutritional needs of elderly consumers through product formats that they want. The heterogeneity of the elderly market poses a challenge, however, and calls for market segmentation. Although many researchers have proposed ways to segment the elderly consumer population, the elderly food market has received surprisingly little attention in this respect. Therefore, the present paper reviewed eight potential segmentation bases on their appropriateness in the context of functional foods aimed at the elderly: cognitive age, life course, time perspective, demographics, general food beliefs, food choice motives, product attributes and benefits sought, and past purchase. Each of the segmentation bases had strengths as well as weaknesses regarding seven evaluation criteria. Given that both product design and communication are useful tools to increase the appeal of functional foods, we argue that elderly consumers in this market may best be segmented using a preference-based segmentation base that is predictive of behaviour (for example, attributes and benefits sought), combined with a characteristics-based segmentation base that describes consumer characteristics (for example, demographics). In the end, the effectiveness of (combinations of) segmentation bases for elderly consumers in the functional food market remains an empirical matter. We hope that the present review stimulates further empirical research that substantiates the ideas presented in this paper.
- Published
- 2014
31. Encouraging vegetable intake as a snack among children: the influence of portion and unit size
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van Kleef, E., Bruggers, I., de Vet, E., van Kleef, E., Bruggers, I., and de Vet, E.
- Abstract
OBJECTIVE: People eat more unhealthy foods when served more (portion size effect) and when the food is served in larger units (unit size effect). The present study aimed to examine whether these effects can be used for the good: to increase vegetable consumption among children. DESIGN: A 2×2 between-subjects experiment was conducted at two schools. Pupils were presented in class with cucumber that varied both in unit size (one piece v. pre-sliced) and portion size (one-third v. two-thirds of a cucumber). Children ate ad libitum during the morning break and filled in a survey. SETTING: Primary schools in the centre of the Netherlands. SUBJECTS: Primary-school pupils (n 255) aged 8-13 years. RESULTS: Children ate 54 % more cucumber when served a large compared with a smaller portion (difference of 49 g; P
- Published
- 2015
32. Using a verbal prompt to increase protein consumption in a hospital setting: a field study
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van der Zanden, L.D.T., van Essen, H., van Kleef, E., de Wijk, R.A., van Trijp, J.C.M., van der Zanden, L.D.T., van Essen, H., van Kleef, E., de Wijk, R.A., and van Trijp, J.C.M.
- Abstract
Background: Sufficient protein intake among hospitalized patients may contribute to faster recovery and a decrease in healthcare costs. Nevertheless, hospitalized patients are often found to consume too little protein. This field study explored the success of a small, inexpensive intervention adapted from the marketing literature, to encourage protein consumption among hospitalized patients. Methods: The study was performed at a hospital where patients order food by calling to the meal service. The intervention consisted of a verbal prompt: “Would you like some [target product] with that?”, which was presented to patients by trained telephone operators, after patients finished ordering their lunch. Target products were two foods rich in protein; fruit quark and yoghurt drink. For half of the patients, the verbal prompt was preceded by verbal praise on their lunch order, which was aimed to increase compliance with the verbal prompt. Results: Three hundred and fifteen hospitalized patients, aged 18–87 years took part in the study. Verbal prompts significantly increased ordering of the target products nearly sevenfold (from ordering by 6.5 % of patients to 45.2 % of patients). Protein content of ordered lunch and all food orders of the day combined showed a trend, with orders of patients receiving only a verbal prompt or a verbal prompt and verbal praise containing a larger amount of protein than lunch orders of patients in the control condition. At an individual level, protein content of ordered food increased significantly, reaching the 25–30 g of protein per main meal recommended by dieticians of the hospital. Verbal praise did not increase compliance with the verbal prompt. Patients consumed most or all of the target product and verbal prompts were not perceived to be obtrusive. Conclusions: Although changing eating patterns is challenging, this study shows that simple interventions such as verbal prompts may be useful tools for health professionals to stimulate he
- Published
- 2015
33. Food risk management practices: the views of consumers and experts
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Houghton, J.R., van Kleef, E., Rowe, G., and Frewer, L.J.
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Marketing and Consumer Behaviour ,MGS ,Life Science ,Marktkunde en Consumentengedrag - Published
- 2006
34. Consumer research in the early stages of new product development : issues and applications in the food domain
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van Kleef, E., Wageningen University, Hans van Trijp, and Pieternel Luning
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Marketing and Consumer Behaviour ,consumer affairs ,consumentenaangelegenheden ,functionele voedingsmiddelen ,research ,productontwikkeling ,Leerstoelgroep Productontwerpen en kwaliteitskunde ,consumenten ,Product Design and Quality Management Group ,onderzoek ,consumers ,new products ,nieuwe producten ,Marktkunde en Consumentengedrag ,gezondheidsvoedsel ,product development ,health foods ,functional foods ,VLAG - Abstract
New products that deliver added consumer value contribute significantly to the success of companies. In the numerous studies of new product performance over the years, consensus has developed that understanding consumer needs is of paramount strategic value, especially in the early stages of the product development process. During these early stages, the product has not yet been specified and the aim is to search for novel product ideas from a marketing and technological perspective. Despite their importance, several studies indicate that consumer research methodologies are underutilised in the early stages of new product development. The aim of this thesis is to analyse key issues and develop and illustrate appropriate consumer research methodology at early stages of the new product development process, as this is one of the most distinguishing characteristics of successful product development projects.Consumer research can be confirmative in its focus of testing new product concepts before launch and in this way prevents unjustified investments. Consumer research can also be proactive in that it aims to identify new product ideas that deliver against consumer needs that are not yet fulfilled by products currently in the market. Successful new product development requires a balance between both types of consumer research. The research in this thesis focuses on the optimal application of both types of consumer research in the early stages of the development process, in particular in providing guidance in generating and validating new product concepts. In the first chapter, the importance of new product development is presented and key factors of success and failure are discussed. Specially, the need for consumer research in the early stages is considered and criteria for effective strategic consumer research are outlined.In chapter 2, ten frequently used methods and techniques to uncover unmet consumer needs and wants are critically reviewed. Each of the following empirical chapters focuses on a specific aspect of the problems associated with selecting and implementing appropriate consumer research in the early stages of the product development process. Chapter 3 presents a framework which allows obtaining relevant consumer and expert feedback in an early stage of the product development process. By systematically generating and rigorously screening a large set of product concepts both inside (experts) and outside (consumers) the company, the framework shows the extent to which experts and consumer agree about new product opportunities and in this way prevents that high potential opportunities are overlooked.Chapter 4 illustrates the problem of successful functional food innovation. This chapter provides insight in a number of strategic decisions that have to be taken in the early stages of the development process in relation to health claim formulation, segment determination and product selection. Chapter 5 provides a comprehensive conceptual and empirical comparison of internal and external preference analysis. In addition to a comparison on statistical criteria, this study explicitly takes the end-user perspective into account by comparing both techniques on various end-user criteria. The final empirical chapter in this thesis (chapter 6) studies the added value of the innovation templates approach in generating and screening new product ideas. Chapter 7 summarizes the results of the previous chapters and describes the limitations of this thesis. Overall, the results of this thesis contribute to the better recognition of the importance of consumer research in early stages of new product development and suggest methodologies that could support effective marketing-R&D interfacing early in the process.
- Published
- 2006
35. Consumentenonderzoek in een vroeg stadium van het productontwikkelingsproces
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van Kleef, E.
- Subjects
innovations ,productontwikkeling ,product development ,consumer surveys ,consumentenonderzoeken ,innovaties - Published
- 2005
36. Nudging children towards whole wheat bread: a field experiment on the influence of fun bread roll shape on breakfast consumption
- Author
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van Kleef, E., Vrijhof, M.N., Polet, I.A., Vingerhoeds, M.H., de Wijk, R.A., van Kleef, E., Vrijhof, M.N., Polet, I.A., Vingerhoeds, M.H., and de Wijk, R.A.
- Abstract
Background: Many children do not eat enough whole grains, which may have negative health consequences. Intervention research is increasingly focusing on nudging as a way to influence food choices by affecting unconscious behavioural processes. The aim of this field study was to examine whether the shape of bread rolls is able to shift children’s bread choices from white to whole wheat during breakfast to increase whole grain intake. Methods: In a between-subjects experiment conducted at twelve primary schools in the Netherlands, with school as the unit of condition assignment, children were exposed to an assortment of white and whole wheat bread rolls, both varying in shape (regular versus fun). Children were free to choose the type and number of bread rolls and toppings to eat during breakfast. Consumption of bread rolls was measured at class level via the number of bread rolls before and after breakfast. In addition, children (N = 1113) responded to a survey including questions about the breakfast. Results: Results of the field experiment showed that about 76% of bread consumption consisted of white bread rolls. Consumption of white bread rolls did not differ according to shape (all P-values > 0.18). However, presenting fun-shaped whole wheat bread rolls almost doubled consumption of whole wheat bread (P = 0.001), particularly when the simultaneously presented white bread rolls had a regular shape (interaction P = 0.02). Survey results suggest that slight increases in perceived pleasure and taste are associated with these effects. Conclusions: Overall, presenting whole wheat bread in fun shapes may be helpful in increasing consumption of whole wheat bread in children. Future research could examine how improving the visual appeal of healthy foods may lead to sustained behaviour changes. Keywords: Nudge, Nudging, Whole grain, Whole-wheat bread, Children’s food preferences, Bread shape, Visual appeal
- Published
- 2014
37. Successful development of satiety enhancing food products: towards a multidisciplinary agenda of research challenges
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van Kleef, E., van Trijp, J.C.M., van den Borne, J.J.G.C., Zondervan, C., van Kleef, E., van Trijp, J.C.M., van den Borne, J.J.G.C., and Zondervan, C.
- Abstract
In the context of increasing prevalence of overweight and obesity in societies worldwide, enhancing the satiating capacity of foods may help people control their energy intake and weight. This requires an integrated approach between various food related disciplines. By structuring this approach around the new product development process, this paper aims to present the contours of such an integrative approach by going through the current state of the art around satiety enhancing foods. It portrays actual food choice as the end result of a complex interaction between internal satiety signals, other food benefits and environmental cues. Three interrelated routes to satiating enhancement are (1) change food composition to develop stronger physiological satiation and satiety signals, (2) anticipate and build on smart external stimuli at moment of purchase and consumption, and (3) improve palatability and acceptance of satiety enhanced foods. Key research challenges in achieving those routes in the field of nutrition, food technology, consumer, marketing and communication are outlined
- Published
- 2012
38. Healthy snacks at the checkout counter: A lab and field study on the impact of shelf arrangement and assortment structure on consumer choices
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van Kleef, E., Otten, K.K., van Trijp, J.C.M., van Kleef, E., Otten, K.K., and van Trijp, J.C.M.
- Abstract
Background: The essence of nudging is to adapt the environment in which consumers make decisions to help them make better choices, without forcing certain outcomes upon them. To determine how consumers can effectively be guided to select healthier snacks, we examine the effect of manipulating the assortment structure and shelf layout of an impulse display including both healthy and unhealthy snacks near the checkout counter of a canteen. Methods: Both a lab and field study applied a two-factor experimental design manipulating snack offerings both in an on-screen choice environment and a natural environment (hospital staff restaurant). Shelf arrangement (i.e. accessibility) was altered by putting healthy snacks at higher shelves versus lower shelves. Assortment structure (i.e. availability) was altered by offering an assortment that either included 25% or 75% healthy snacks. Participants in the lab study (n = 158) made a choice from a shelf display. A brief survey following snack selection asked participants to evaluate the assortment and their choice. The field experiment took place in a hospital canteen. Daily sales data were collected for a period of four weeks. On completion of the field study, employees (n = 92) filled out a questionnaire about all four displays and rated their attractiveness, healthiness and perceived freedom of choice. Results: The lab study showed a higher probability of healthy snack choice when 75% of the assortment consisted of healthy snacks compared to conditions with 25% healthy snack assortments, even though choices were not rated less satisfying or more restrictive. Regarding shelf display location of healthy snacks, no significant differences were observed. There was also no significant shelf arrangement by assortment structure interactive effect. The field study replicated these findings, in that this assortment structure led to higher sales of healthy snacks. Sales of unhealthy and total snacks were not impacted by manipulations (n
- Published
- 2012
39. P2-366 The global distribution of dengue: Past, present and future impacts of climate change
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Van Kleef, E., Bambrick, Hilary, Hales, S., Van Kleef, E., Bambrick, Hilary, and Hales, S.
- Abstract
Approximately 2.5 billion people are at risk from dengue. Climate change could drive the epidemic potential of this disease through temperature impacts on both the virus and the mosquito vector. We describe the past and present global distribution of transmission and estimate possible changes to dengue distribution arising from climate change.
- Published
- 2011
40. Food compensation: do exercise ads change food intake?
- Author
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van Kleef, E., Shimizu, M., Wansink, B., van Kleef, E., Shimizu, M., and Wansink, B.
- Abstract
Background: Past research has shown that promotional messages such as food advertising influence food consumption. However, what has gone largely unexplored is the effect of exercise advertising on food intake. This study experimentally tested the effects of exposure to exercise commercials on food intake at a lunch meal as compared to the effects of control commercials. Methods: Prior to eating lunch, 125 participants (71 women, 54 men) watched 8 commercials, either all related to exercise or fitness (n = 67) or neutral products (i.e. car insurance) (n = 58). The meal consisted of a pasta dish with tomato sauce, salad and chocolate pudding. The post-lunch questionnaire included questions about body mass index, exercise habits, motivation and dietary restraint. Results: Participants exposed to exercise commercials reduced their caloric intake by 21.7% relative to the control condition. Additionally, watching exercise messages increased the perceived healthiness and liking of the meal. Although exercise habits and intentions did not moderate the effect of commercial condition on food intake, we also found that this intake reduction was driven by participants with higher body mass index levels. Conclusions: These results imply that exercise messages may serve as a reminder of the link between food and physical activity and affect food consumption. It also highlights the need for increased awareness that these messages have powerful influences not only on exercise behavior, but also on closely related behaviors such as eating
- Published
- 2011
41. Consumer preferences for front-of-pack calories labelling
- Author
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van Kleef, E., van Trijp, J.C.M., Paeps, F., Fernández-Celemín, L., van Kleef, E., van Trijp, J.C.M., Paeps, F., and Fernández-Celemín, L.
- Abstract
Objective In light of the emerging obesity pandemic, front-of-pack calories labels may be an important tool to assist consumers in making informed healthier food choices. However, there is little prior research to guide key decisions on whether caloric content should be expressed in absolute terms or relative to recommended daily intake, whether it should be expressed in per serving or per 100 g and whether the information should be further brought alive for consumers in terms of what the extra calorie intake implies in relation to activity levels. The present study aimed at providing more insight into consumers¿ appreciation of front-of-pack labelling of caloric content of food products and their specific preferences for alternative execution formats for such information in Europe. Design For this purpose, eight executions of front-of-pack calorie flags were designed and their appeal and information value were extensively discussed with consumers through qualitative research in four different countries (Germany, The Netherlands, France and the UK). Results The results show that calories are well-understood and that participants were generally positive about front-of-pack flags, particularly when flags are uniform across products. The most liked flags are the simpler flags depicting only the number of calories per serving or per 100 g, while more complex flags including references to daily needs or exercise and the flag including a phrase referring to balanced lifestyle were least preferred. Some relevant differences between countries were observed. Although participants seem to be familiar with the notion of calories, they do not seem to fully understand how to apply them. Conclusion From the results, managerial implications for the design and implementation of front-of-pack calorie labelling as well as important directions for future research are discussed.
- Published
- 2008
42. Consumer research in the early stages of new product development : issues and applications in the food domain
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van Trijp, Hans, Luning, Pieternel, van Kleef, E., van Trijp, Hans, Luning, Pieternel, and van Kleef, E.
- Abstract
New products that deliver added consumer value contribute significantly to the success of companies. In the numerous studies of new product performance over the years, consensus has developed that understanding consumer needs is of paramount strategic value, especially in the early stages of the product development process. During these early stages, the product has not yet been specified and the aim is to search for novel product ideas from a marketing and technological perspective. Despite their importance, several studies indicate that consumer research methodologies are underutilised in the early stages of new product development. The aim of this thesis is to analyse key issues and develop and illustrate appropriate consumer research methodology at early stages of the new product development process, as this is one of the most distinguishing characteristics of successful product development projects.Consumer research can be confirmative in its focus of testing new product concepts before launch and in this way prevents unjustified investments. Consumer research can also be proactive in that it aims to identify new product ideas that deliver against consumer needs that are not yet fulfilled by products currently in the market. Successful new product development requires a balance between both types of consumer research. The research in this thesis focuses on the optimal application of both types of consumer research in the early stages of the development process, in particular in providing guidance in generating and validating new product concepts. In the first chapter, the importance of new product development is presented and key factors of success and failure are discussed. Specially, the need for consumer research in the early stages is considered and criteria for effective strategic consumer research are outlined.In chapter 2, ten frequently used methods and techniques to uncover unmet consumer needs and wants are critically reviewed. Each of the following e
- Published
- 2006
43. P2-366 The global distribution of dengue: past, present and future impacts of climate change
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van Kleef, E., primary, Bambrick, H., additional, and Hales, S., additional
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- 2011
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44. Alpha 1-adrenoreceptor blockade reduces the angiotensin II-induced vascular smooth muscle cell DNA synthesis in the rat thoracic aorta and carotid artery
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van Kleef, E. M., Smits, J. F., de Mey, J. G., Cleutjens, J. P., Lombardi, D. M., Schwartz, S. M., Daemen, M. J., and Other departments
- Subjects
cardiovascular system - Abstract
We explored effects of alpha 1-adrenoreceptor blockade with prazosin on the increased vascular smooth muscle cell (SMC) DNA synthesis induced by angiotensin II (Ang II) in rats. Ang II was infused with or without prazosin or its solvent. Observations were compared with those in rats receiving saline or solvent. In group A, Ang II was infused for 2 weeks by subcutaneously implanted osmotic minipumps at a rate of 35 ng/100 g per minute. Group B received Ang II together with the alpha 1-adrenoreceptor antagonist prazosin (0.35 micrograms/100 g per minute). Group C received Ang II and 50% dimethyl sulfoxide (DMSO), the solvent of prazosin; group D received 50% DMSO; and group E received 0.9% NaCl (Ang II vehicle). All animals were infused with 5-bromo-2'-deoxyuridine for 2 weeks via separate minipumps to measure DNA synthesis. Ang II significantly increased the fraction of DNA synthesizing SMCs in the media of the thoracic aorta from 0.4 +/- 0.1% (mean +/- SD) in group E (n = 6) to 10.8 +/- 7.0% in group A (n = 8). Addition of prazosin to Ang II reduced the labeling fraction of SMCs to 3.0 +/- 2.2% (group B, n = 9). The remaining SMC DNA synthesis in the prazosin-treated group was probably due to the effects of the solvent of prazosin, i.e., 50% DMSO, since infusion of 50% DMSO alone increased the labeling fraction to 4.1 +/- 2.0% (group D, n = 6).(ABSTRACT TRUNCATED AT 250 WORDS)
- Published
- 1992
45. Aircraft Simulation and Pilot Proficiency: From Surrogate Flying Towards Effective Training
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NATIONAL AEROSPACE LAB AMSTERDAM (NETHERLANDS), Jorna, P. G., van Kleef, E. R., de Boer, W. P., NATIONAL AEROSPACE LAB AMSTERDAM (NETHERLANDS), Jorna, P. G., van Kleef, E. R., and de Boer, W. P.
- Abstract
Simulators are currently build as simple system 'look-a-likes' and a structured or experimentally validated approach to their use and implementation as part of a comprehensive training system is lacking. This is one reason why their use in training has not always been as successful as expected. Training time in an aircraft can be reduced to a certain extent, but most often at the cost of increasing total time due to imperfect transfer ratios. The other reason for limited success is that objective and validated indices for performance are rarely available which complicates both the definition of measurable training objectives and the empirical identification of task characteristics critical for skill development. Without objective indices, the issue of 'fidelity' versus 'validity' remains unresolved., This article is from 'Piloted Simulation Effectiveness Held in Brussels, Belgium on 14-17 October 1991 (L'Efficacite de la Simulation Pilotee),' AD-A253 007, p10-1 thru 10-8.
- Published
- 1992
46. Doxazosin blocks the angiotensin II-induced smooth muscle cell DNA synthesis in the media, but not in the neointima of the rat carotid artery after balloon injury
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Van Kleef, E, primary
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- 1996
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47. Consumer preferences for front-of-pack calories labelling.
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van Kleef E, van Trijp H, Paeps F, Fernández-Celemín L, van Kleef, Ellen, van Trijp, Hans, Paeps, Frederic, and Fernández-Celemín, Laura
- Abstract
Objective: In light of the emerging obesity pandemic, front-of-pack calories labels may be an important tool to assist consumers in making informed healthier food choices. However, there is little prior research to guide key decisions on whether caloric content should be expressed in absolute terms or relative to recommended daily intake, whether it should be expressed in per serving or per 100 g and whether the information should be further brought alive for consumers in terms of what the extra calorie intake implies in relation to activity levels. The present study aimed at providing more insight into consumers' appreciation of front-of-pack labelling of caloric content of food products and their specific preferences for alternative execution formats for such information in Europe.Design: For this purpose, eight executions of front-of-pack calorie flags were designed and their appeal and information value were extensively discussed with consumers through qualitative research in four different countries (Germany, The Netherlands, France and the UK).Results: The results show that calories are well-understood and that participants were generally positive about front-of-pack flags, particularly when flags are uniform across products. The most liked flags are the simpler flags depicting only the number of calories per serving or per 100 g, while more complex flags including references to daily needs or exercise and the flag including a phrase referring to balanced lifestyle were least preferred. Some relevant differences between countries were observed. Although participants seem to be familiar with the notion of calories, they do not seem to fully understand how to apply them.Conclusion: From the results, managerial implications for the design and implementation of front-of-pack calorie labelling as well as important directions for future research are discussed. [ABSTRACT FROM AUTHOR]- Published
- 2008
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48. Modelling studies of the transmission-dynamics and\ud hospital burden of Clostridium difficile
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van Kleef, E, Edmunds, John, Robotham, J, Deeny, Sarah, and Jit, M
- Abstract
Clostridium difficile, a Gram-positive spore-forming bacterium, is a source of considerable morbidity\ud and mortality for patients treated in hospitals and other healthcare settings. Intestinal colonisation by C.\ud difficile can cause infection (CDI) if the normal flora is disrupted, e.g. by the use of antimicrobials and\ud some other drugs. Vaccines targeting C difficile main virulence factors, toxins A and B are currently\ud undergoing clinical trials, however, their potential population impact is largely unknown. The work\ud presented in this thesis aims to quantify the effectiveness of C. difficile vaccination in preventing hospitalonset\ud CDI, including both its direct effects (reduction in individual patient morbidity and mortality) and\ud indirect effects (prevention of onward transmission of the bacteria) using a mathematical dynamic\ud transmission model framework.\ud Based on a systematic literature review, it was shown that mathematical dynamic-transmission models\ud have become an increasingly popular tool to help understand the patient-to-patient spread of nosocomial\ud pathogens and predict the impact of healthcare prevention and control strategies. Methods have generally\ud improved, with an increased use of stochastic models, and more advanced methods for formal model\ud fitting and sensitivity analyses. Nonetheless, in contrast to methicillin-resistant Staphylococcus aureus –\ud another bacterium commonly found in the healthcare setting – the transmission of C. difficile has rarely\ud been considered within a dynamic modelling framework.\ud Using national English CDI hospital surveillance data to fit a generalised additive mixed-effects\ud model, this thesis revealed that, in line with recent evidence based on highly discriminatory genetic typingmethods,\ud whilst transmission between symptomatic carriers was significant, this did not account for the\ud majority of CDI cases in English hospitals. Asymptomatic carriers have been suggested as cocontributors,\ud but their role in transmission remains uncertain to date.\ud Previous estimates of additional excess bed days attributable to healthcare-acquired-CDI have varied\ud widely, partly due to methodological weaknesses, and no robust estimates from a European setting are\ud available. Both form key determinants to help quantify the health and economic burden of CDI, and are\ud also likely to have an impact on the transmission-dynamics of the infection. Therefore, this thesis\ud quantified the hospital burden of CDI, expressed in excess length of stay and mortality. A Cox\ud proportional hazard model revealed that CDI was associated with a significantly decreased daily risk of\ud discharge and increased risk of mortality, where the former was even further reduced for severe CDI\ud patients. Using a multi-state model more intuitive estimates, i.e. the excess length of stay associated with\ud mild (5 days [1.1-9.5]) and severe CDI (11.6 days [95% CI = 3.6-19.6]) were obtained.\ud Finally, the results of an individual-based “state-of-the-art” dynamic transmission model in an\ud English ICU (with epidemiological parameters informed by the findings of the statistical models\ud mentioned, and with data-driven patient movement between the community, LTCF and ICU) showed\ud that in settings with in-hospital acquisition rates comparable to the national average in English ICUs,\ud immunising three patient groups: LTCF residents, elective patients and patients with a history of CDI in\ud the ICU, resulted in a 43%, reduction of ICU-onset CDI. This required a relatively high number of\ud vaccine doses, and a targeted strategy involving patients at high risk of colonisation on admission, such as\ud LTCF residents proved more efficient. As these results proved highly sensitive to the level of\ud antimicrobial use and in-ward acquisition rates, it was concluded that vaccination might be most efficient\ud when targeting patient risk groups or settings where implementation of antimicrobial stewardship proves\ud challenging.
49. Embedding risk monitoring in infectious disease surveillance for timely and effective outbreak prevention and control.
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Ingelbeen B, van Kleef E, Mbala P, Danis K, Macicame I, Hens N, Cleynen E, and van der Sande MAB
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- Humans, Communicable Diseases epidemiology, Communicable Disease Control methods, Population Surveillance methods, Risk Assessment, Disease Outbreaks prevention & control
- Abstract
Epidemic intelligence efforts aim to predict, timely detect and assess (re-)emerging pathogens, guide and evaluate infectious disease prevention or control. We emphasise the underused potential of integrating the monitoring of risks related to exposure, disease or death, particularly in settings where limited diagnostic capacity and access to healthcare hamper timely prevention/control measures. Monitoring One Health exposures, human behaviour, immunity, comorbidities, uptake of control measures or pathogen characteristics can complement facility-based surveillance in generating signals of imminent or ongoing outbreaks, and in targeting preventive/control interventions or epidemic preparedness to high-risk areas or subpopulations. Low-cost risk data sources include electronic medical records, existing household/patient/environmental surveys, Health and Demographic Surveillance Systems, medicine distribution and programmatic data. Public health authorities need to identify and prioritise risk data that effectively fill gaps in intelligence that facility-based surveillance can not timely or accurately answer, determine indicators to generate from the data, ensure data availability, regular analysis and dissemination., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2025. Re-use permitted under CC BY. Published by BMJ Group.)
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- 2025
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50. Mild and moderate COVID-19 during Alpha, Delta and Omicron pandemic waves in urban Maputo, Mozambique, December 2020-March 2022: A population-based surveillance study.
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Ingelbeen B, Cumbane V, Mandlate F, Barbé B, Nhachungue SM, Cavele N, Manhica C, Cubai C, Nguenha NMC, Lacroix A, Mariën J, de Weggheleire A, van Kleef E, Selhorst P, van der Sande MAB, Peeters M, Widdowson MA, Ismael N, and Macicame I
- Abstract
In sub-Saharan Africa, reported COVID-19 numbers have been lower than anticipated, even when considering populations' younger age. The extent to which risk factors, established in industrialised countries, impact the risk of infection and of disease in populations in sub-Saharan Africa, remains unclear. We estimated the incidence of mild and moderate COVID-19 in urban Mozambique and analysed factors associated with infection and disease in a population-based surveillance study. During December 2020-March 2022, 1,561 households (6,049 participants, median 21 years, 54.8% female, 7.3% disclosed HIV positive) of Polana Caniço, Maputo, Mozambique, were visited biweekly to report respiratory symptoms, anosmia, or ageusia, and self-administer a nasal swab for SARS-CoV-2 testing. Every three months, dried blood spots of a subset of participants (1,412) were collected for detection of antibodies against SARS-CoV-2 spike glycoprotein and nucleocapsid protein. Per 1000 person-years, 364.5 (95%CI 352.8-376.1) respiratory illness episodes were reported, of which 72.2 (95%CI 60.6-83.9) were COVID-19. SARS-CoV-2 seroprevalence rose from 4.8% (95%CI 1.1-8.6%) in December 2020 to 34.7% (95%CI 20.2-49.3%) in June 2021, when 3.0% were vaccinated. Increasing age, chronic lung disease, hypertension, and overweight increased risk of COVID-19. Older age increased the risk of SARS-CoV-2 seroconversion. We observed no association between socio-economic status, behaviour and COVID-19 or SARS-CoV-2 seroconversion. Active surveillance in an urban population confirmed frequent COVID-19 underreporting, yet indicated that the large majority of cases were mild and non-febrile. In contrast to reports from industrialised countries, social deprivation did not increase the risk of infection nor disease., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Ingelbeen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
- Full Text
- View/download PDF
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