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This cross-sectional study examined gastrocnemius medialis fascicle length (FL) and anatomical cross-sectional area (ACSA) in female volleyball athletes aged 8–18 years, in an attempt to investigate morphological growth changes during childhood and adolescence. Methods: Eighty-nine athletes were assigned to five groups: 8–10, 10–12, 12–14, 14–16 and 16–18 years old. Height, body mass, leg and calf length were measured, and maturity offset was calculated from anthropometrics. FL and ACSA were assessed using ultrasonography. Results: FL was longer and ACSA was larger in the 12–14 y group compared with the 8–10 y group (4.31 ± 0.60 vs. 3.66 ± 0.40 cm, p = 0.008 and 13.08 ± 3.01 cm2 vs. 9.98 ± 2.07 cm2, p = 0.018, respectively). However, there were no differences in FL or ACSA among the groups aged between 12 and 18 years (p > 0.573 and p > 0.352). Notably the percentage increase in ACSA between the youngest (8–10 y) and the oldest group (16–18 y) was twofold greater than the percentage increase in FL (49% vs. 24%). Significant correlations were found between anthropometric characteristics FL and ACSA (r = 0.366–760, p < 0.001). Conclusions: These data indicate that there is a rapid increase in FL and ACSA from the age of 8 y until the age of 14 y, which is a crucial time frame for muscle growth. [ABSTRACT FROM AUTHOR]

Abstract Background Musculoskeletal alterations causing reduced range of motion of the ankle joint are common in children with cerebral palsy (CP). Objective measurements of passive joint resistance and three-dimensional skeletal muscle volume and muscle architecture can lead to a comprehensive understanding of which factors influence joint range of motion. Research question To investigate the relation between the passive dorsiflexion of the ankle joint, biomechanical contributing factors to the passive joint resistance, and muscular architectural properties of the triceps surae muscle in children with CP. Methods In this cross-sectional observational study, 14 children with spastic CP (bilateral: 5, unilateral: 9, Gross Motor Function Classification System (GMFCS) level I:11, II:3) naïve to intramuscular tone reducing treatment, and 14 TD children were included. The passive dorsiflexion of the ankle was measured with a goniometer. Passive joint resistance and related parameters were estimated based on a biomechanical model and measurements using a motorized device, the Neuroflexor. Three-dimensional muscle architecture was quantified with diffusion tensor magnetic resonance imaging (DT-MRI). Results In the CP group, the median [min, max] passive dorsiflexion was decreased in the most affected leg (MAL) compared to the less affected leg (LAL) (2.5° [-25°, 20°] vs. 12.5° [5°, 30°], p = 0.001). The stiffness coefficient (Nm/rad) in the MAL was significantly higher in children with CP compared to TD children (7.10 [3.39, 62.00] vs. 2.82 [1.24, 10.46], p = 0.015). Muscle architecture properties did not differ between CP and TD, except for pennation angle in the medial gastrocnemius (MG) of the MAL (CP 17.64° (2.29) vs. TD 21.46° (3.20), p = 0.017). The stiffness coefficient, in the MAL, correlated negatively to passive dorsiflexion (rs=-0.638) and pennation angle in medial gastrocnemius (rs=-0.964), and the non-linear coefficient (Non-linear 1) correlated negatively to the fascicle length of the medial gastrocnemius (rs=-0.857). Conclusion This study shows that stiffness of the plantarflexors is related to decreased passive dorsiflexion of the ankle and muscle structure of the MG in high-functioning children with spastic CP. Assessments of how dynamic components as well as microscopic muscle alterations contribute to joint stiffness in the plantarflexors in individuals with CP are warranted. Trial registration Retrospectively registered in ClinicalTrials.gov, NCT05447299. Observational study. Study start: 2019-01-15, register date: 2022-07-01.

Paul Ritsche,1 Ralf Roth,1 Thomas Bernhard,2 Lukas Nebiker,1 Eric Lichtenstein,1 Martino Franchi,3 Jörg Spörri,4,5 Oliver Faude1 1Department of Sport, Exercise and Health, University of Basel, Basel, Switzerland; 2FC Basel 1893, Basel, Switzerland; 3Human Neuromuscular Physiology Lab, Department of Biomedical Sciences, University of Padua, Padova, Italy; 4Sports Medical Research Group, Department of Orthopaedics, Balgrist University Hospital, University of Zurich, Zurich, Switzerland; 5University Centre for Prevention and Sports Medicine, Department of Orthopaedics, Balgrist University Hospital, University of Zurich, Zurich, SwitzerlandCorrespondence: Paul Ritsche, University of Basel, Department of Sport, Exercise and Health, Grosse Allee 6, Basel, 4052, Switzerland, Email Paul.ritsche@unibas.chPurpose: Adolescent soccer players experience distinct physiological changes due to chronological and biological maturation, impacting their soccer performance. Here, we explored age-related variations and associations between quadriceps geometry and strength in male national-level adolescent soccer players.Patients and Methods: We used ultrasonography to examine the regional architecture and morphology of the rectus femoris (RF) and vastus lateralis (VL) muscles, and we assessed knee extension strength by isometric and isokinetic dynamometry. Players were categorized into four age groups: under (U) 15 (n=18, age=13.7± 0.5 years), U16 (n=15, age=14.7± 0.5), U17 (n=19, age=15.7± 0.5), U18 (n=18, age=16.7± 0.5) and U21 (n=25, age=18.5± 0.5).Results: The absolute and relative strengths were higher in the U16 compared to U15 by 12– 15% and 6– 8%, 11– 12% and 6– 7% in the U17 compared to U16, 5– 7% and − 1– 2% in the U18 compared to U17 and 0– 15% and − 1– 11% in the U21 compared to U18 age groups, respectively. VL architecture did not change relevantly between the age groups. The muscle anatomical cross-sectional area (ACSA) of the VL and RF differed non-uniformly and muscle region-specific by 10– 36%, with highest values in the U21 age group. Moderate correlations between the VL architecture and knee extension strength in both legs were observed only in the U16 age group. The quadriceps ACSA showed age-specific correlations with knee extension strength.Conclusion: Our findings highlight non-uniform differences in quadriceps muscle morphology and absolute and relative strength among male national-level adolescent soccer players in different age groups. The correlations observed between muscle morphology or architecture and strength were muscle, muscle region, leg and age dependent.Keywords: strength, youth, muscle architecture, vastus lateralis, rectus femoris

Abstract During muscle contraction, not only are the fascicles shortening but also the pennation angle changes, which leads to a faster contraction of the muscle than of its fascicles. This phenomenon is called muscle gearing, and it has a direct influence on the force output of the muscle. There are few studies showing pennation angle changes during isometric and concentric contractions for different contraction intensities and muscle lengths. Therefore, the aim was to determine these influences over a wide range of contraction intensities and ankle joint angles for human triceps surae. Additionally, the influence of contraction intensity and ankle joint angle on muscle gearing was evaluated. Ten sport students performed concentric and isometric contractions with intensities between 0 and 90% of the maximum voluntary contraction and ankle joint angles from 50° to 120°. During these contractions, the m. gastrocnemius medialis and lateralis and the m. soleus were recorded via ultrasound imaging. A nonlinear relationship between fascicle length and pennation angle was discovered, which can be described with a quadratic fit for each of the muscles during isometric contraction. A nearly identical relationship was detected during dynamic contraction. The muscle gearing increased almost linearly with contraction intensity and ankle joint angle.

Abstract Background Older adults exhibit a slower recovery of muscle mass following disuse atrophy than young adults. At a smaller scale, muscle fibre cross-sectional area (i.e., sarcomeres in parallel) exhibits this same pattern. Less is known, however, about age-related differences in the recovery of muscle fibre length, driven by increases in serial sarcomere number (SSN), following disuse. The purpose of this study was to investigate age-related differences in SSN adaptations and muscle mechanical function during and following muscle immobilization. We hypothesized that older adult rats would experience a similar magnitude of SSN loss during immobilization, however, take longer to recover SSN than young following cast removal, which would limit the recovery of muscle mechanical function. Methods We casted the plantar flexors of young (8 months) and old (32 months) male rats in a shortened position for 2 weeks, and assessed recovery during 4 weeks of voluntary ambulation. Following sacrifice, legs were fixed in formalin for measurement of soleus SSN and physiological cross-sectional area (PCSA) with the un-casted soleus acting as a control. Ultrasonographic measurements of pennation angle (PA) and muscle thickness (MT) were conducted weekly. In-vivo active and passive torque-angle relationships were constructed pre-cast, post-cast, and following 4 weeks of recovery. Results From pre- to post-cast, young and older adult rats experienced similar decreases in SSN (–20%, P

Background: Musculoskeletal alterations causing reduced range of motion of the ankle joint are common in children with cerebral palsy (CP). Objective measurements of passive joint resistance and three-dimensional skeletal muscle volume and muscle architecture can lead to a comprehensive understanding of which factors influence joint range of motion. Research question: To investigate the relation between the passive dorsiflexion of the ankle joint, biomechanical contributing factors to the passive joint resistance, and muscular architectural properties of the triceps surae muscle in children with CP. Methods: In this cross-sectional observational study, 14 children with spastic CP (bilateral: 5, unilateral: 9, Gross Motor Function Classification System (GMFCS) level I:11, II:3) naïve to intramuscular tone reducing treatment, and 14 TD children were included. The passive dorsiflexion of the ankle was measured with a goniometer. Passive joint resistance and related parameters were estimated based on a biomechanical model and measurements using a motorized device, the Neuroflexor. Three-dimensional muscle architecture was quantified with diffusion tensor magnetic resonance imaging (DT-MRI). Results: In the CP group, the median [min, max] passive dorsiflexion was decreased in the most affected leg (MAL) compared to the less affected leg (LAL) (2.5° [-25°, 20°] vs. 12.5° [5°, 30°], p = 0.001). The stiffness coefficient (Nm/rad) in the MAL was significantly higher in children with CP compared to TD children (7.10 [3.39, 62.00] vs. 2.82 [1.24, 10.46], p = 0.015). Muscle architecture properties did not differ between CP and TD, except for pennation angle in the medial gastrocnemius (MG) of the MAL (CP 17.64° (2.29) vs. TD 21.46° (3.20), p = 0.017). The stiffness coefficient, in the MAL, correlated negatively to passive dorsiflexion (rs=-0.638) and pennation angle in medial gastrocnemius (rs=-0.964), and the non-linear coefficient (Non-linear 1) correlated negatively to the fascicle length of the medial gastrocnemius (rs=-0.857). Conclusion: This study shows that stiffness of the plantarflexors is related to decreased passive dorsiflexion of the ankle and muscle structure of the MG in high-functioning children with spastic CP. Assessments of how dynamic components as well as microscopic muscle alterations contribute to joint stiffness in the plantarflexors in individuals with CP are warranted. Trial registration: Retrospectively registered in ClinicalTrials.gov, NCT05447299. Observational study. Study start: 2019-01-15, register date: 2022-07-01. [ABSTRACT FROM AUTHOR]

The age‐related loss of muscle mass is partly accounted for by the loss of sarcomeres in series, contributing to declines in muscle mechanical performance. Resistance training biased to eccentric contractions increases serial sarcomere number (SSN) in young muscle, however, maximal eccentric training in old rats previously did not alter SSN and worsened performance. A submaximal eccentric training stimulus may be more conducive to adaptation for aged muscle. The purpose of this study was to assess whether submaximal eccentric training can increase SSN and improve mechanical function in old rats. Twelve 32‐month‐old male F344/BN rats completed 4 weeks of submaximal (60% maximum) eccentric plantar‐flexion training 3 days/week. Pre‐ and post‐training, we assessed in‐vivo maximum isometric torque at a stretched and neutral ankle angle, the passive torque‐angle relationship, and the isotonic torque‐velocity‐power relationship. The soleus and medial gastrocnemius (MG) were harvested for SSN measurements via laser diffraction, with the untrained leg as a control. SSN increased 11% and 8% in the soleus and MG, respectively. Training also shifted optimal torque production towards longer muscle lengths, reduced passive torque 42%, and increased peak isotonic power 23%. Submaximal eccentric training was beneficial for aged muscle adaptations, increasing SSN, reducing muscle passive tension, and improving dynamic contractile performance. [ABSTRACT FROM AUTHOR]

Simple Summary: "Declawing" is the surgery in which the bone underneath the claw is removed entirely or in part. This has been shown to have substantial effects on the forearm muscles of members of the cat family, but no one has previously examined how it affects other species or the hindlimb. In this study, we examine the leg and forearm muscles of a kinkajou (a Central/South American tree-climbing relative of the raccoon) that was declawed on all four limbs and compare it to several kinkajous that were not declawed and to the previous cat findings. As expected, some of the declawed kinkajou's muscles were substantially different from those of the intact specimens, and as was seen in the cats, the muscles that normally attach to its claw bones appear to have been weaker. Surprisingly, the declawed kinkajou had larger forearm muscles and, even though its toe claws had also been removed, its hindlimb muscles were not very different—possibly because kinkajous rely more on their hands than their feet. Future studies should examine other declawed kinkajous and how this surgery affects other species, like kinkajou relatives that don't climb as much or other species, like monkeys, that do climb like kinkajous. Recently, onychectomy, the "declaw" surgery in which all or part of the distal phalanges are removed, has been shown to have significant effects on the forearm muscles of felids. While this surgery should clearly affect the limb muscles (especially those that insert on the removed or modified bone), these effects have not been studied beyond felids or in the hindlimb. To that end, we herein evaluated the muscle architecture of a kinkajou (Potos flavus) that was declawed on all four of its limbs and compared its anatomy to that of intact specimens and the felid findings. As expected, some of the declawed kinkajou's muscles were substantially different from those of the intact specimens, and as was seen in felids, its digital muscles appear to have been weaker. However, unlike in the felids, the declawed kinkajou had relatively larger forearm muscles. Also, contrary to expectation, the leg muscles of the declawed kinkajou were not substantially different, perhaps reflecting important differences in limb use. Future analyses should examine this anatomy in other declawed kinkajou specimens and also look at the effects of this surgery in other taxa, for instance, non-arboreal relatives of the kinkajou as well as other arboreal taxa. [ABSTRACT FROM AUTHOR]

Background: The supraspinatus is most frequently involved in rotator cuff tears, a common orthopaedic condition. However, the architecture of this muscle has been described only for the superficial, anterior, and posterior regions. Purpose: To determine the muscle architecture of the deep supraspinatus. Study Design: Descriptive laboratory study. Methods: Muscle architecture measurements were collected from 25 cadaveric supraspinatus specimens (13 intact [without tendon tears], 3 with partial-thickness tears, 9 with full-thickness tears). The muscle was divided into deep, superficial anterior, and superficial posterior regions. Pennation angle, raw and normalized fiber length, and sarcomere length and number were compared using repeated-measures analyses of variance. Results: First, mean architecture measurements were compared between regions using only the intact specimens (n = 13). The deep region had a lower mean pennation angle (3.3° ± 1.0°) compared with the posterior region (11.0° ± 3.9°; P <.0001), which in turn had a significantly higher pennation angle compared with the anterior region (7.6 ± 2.6°; P =.0005). Normalized fiber lengths in the deep region were 21.1% (P =.0052) and 34.5% (P <.0001) shorter than the posterior and anterior normalized fiber lengths, respectively. Sarcomere lengths in the deep region were longer (3.4 ± 0.2 μm) compared with the posterior (3.1 ± 0.2 μm; P =.0012) and anterior (3.2 ± 0.2 μm; P =.0390) regions. Sarcomere numbers also decreased in the deep region by 21.2% (P =.0056) and 34.2% (P <.0001) compared with the posterior and anterior regions, respectively. Conclusion: The deep supraspinatus had significantly lower pennation angles, shorter fiber lengths, and fewer but longer sarcomeres in series compared with other subregions within the muscle. These structural differences suggest a functionally unique "submuscle" within the supraspinatus. Clinical Relevance: Understanding the architecture of the supraspinatus muscle can provide insight into muscle function in health and disease. Specifically, this deep submuscle may play a different role in rotator cuff function than the rest of the muscle. [ABSTRACT FROM AUTHOR]

Background: Older adults exhibit a slower recovery of muscle mass following disuse atrophy than young adults. At a smaller scale, muscle fibre cross-sectional area (i.e., sarcomeres in parallel) exhibits this same pattern. Less is known, however, about age-related differences in the recovery of muscle fibre length, driven by increases in serial sarcomere number (SSN), following disuse. The purpose of this study was to investigate age-related differences in SSN adaptations and muscle mechanical function during and following muscle immobilization. We hypothesized that older adult rats would experience a similar magnitude of SSN loss during immobilization, however, take longer to recover SSN than young following cast removal, which would limit the recovery of muscle mechanical function. Methods: We casted the plantar flexors of young (8 months) and old (32 months) male rats in a shortened position for 2 weeks, and assessed recovery during 4 weeks of voluntary ambulation. Following sacrifice, legs were fixed in formalin for measurement of soleus SSN and physiological cross-sectional area (PCSA) with the un-casted soleus acting as a control. Ultrasonographic measurements of pennation angle (PA) and muscle thickness (MT) were conducted weekly. In-vivo active and passive torque-angle relationships were constructed pre-cast, post-cast, and following 4 weeks of recovery. Results: From pre- to post-cast, young and older adult rats experienced similar decreases in SSN (–20%, P < 0.001), muscle wet weight (–25%, P < 0.001), MT (–30%), PA (–15%, P < 0.001), and maximum isometric torque (–40%, P < 0.001), but there was a greater increase in passive torque in older (+ 180%, P < 0.001) compared to young adult rats (+ 68%, P = 0.006). Following cast removal, young exhibited quicker recovery of SSN and MT than old, but SSN recovered sooner than PA and MT in both young and old. PCSA nearly recovered and active torque fully recovered in young adult rats, whereas in older adult rats these remained unrecovered at ∼ 75%. Conclusions: This study showed that older adult rats retain a better ability to recover longitudinal compared to parallel muscle morphology following cast removal, making SSN a highly adaptable target for improving muscle function in elderly populations early on during rehabilitation. [ABSTRACT FROM AUTHOR]

Abstract Both resistance training (RT) and long‐duration, high‐intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well‐trained individuals. A case‐study was conducted on a competitive bodybuilder implementing long‐duration, high‐intensity stretching of the plantar flexors (60 min 6x/week for 12 weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%–23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case‐study nature of our investigation.

Background Brightness-mode (B-mode) ultrasound is a valuable tool to non-invasively image skeletal muscle architectural changes during movement, but automatically tracking muscle fascicles remains a major challenge. Existing fascicle tracking algorithms either require time-consuming drift corrections or yield noisy estimates that require post-processing. We therefore aimed to develop an algorithm that tracks fascicles without drift and with low noise across a range of experimental conditions and image acquisition settings. Methods We applied a Kalman filter to combine fascicle length and fascicle angle estimates from existing and openly-available UltraTrack and TimTrack algorithms into a hybrid algorithm called UltraTimTrack. We applied the hybrid algorithm to ultrasound image sequences collected from the human medial gastrocnemius of healthy individuals (N = 8, four women), who performed cyclical submaximal plantar flexion contractions or remained at rest during passive ankle joint rotations at given frequencies and amplitudes whilst seated in a dynamometer chair. We quantified the algorithm’s tracking accuracy, noise, and drift as the respective mean, cycle-to-cycle variability, and accumulated between-contraction variability in fascicle length and fascicle angle. We expected UltraTimTrack’s estimates to be less noisy than TimTrack’s estimates and to drift less than UltraTrack’s estimates across a range of conditions and image acquisition settings. Results The proposed algorithm yielded low-noise estimates like UltraTrack and was drift-free like TimTrack across the broad range of conditions we tested. Over 120 cyclical contractions, fascicle length and fascicle angle deviations of UltraTimTrack accumulated to 2.1 ± 1.3 mm (mean ± sd) and 0.8 ± 0.7 deg, respectively. This was considerably less than UltraTrack (67.0 ± 59.3 mm, 9.3 ± 8.6 deg) and similar to TimTrack (1.9 ± 2.2 mm, 0.9 ± 1.0 deg). Average cycle-to-cycle variability of UltraTimTrack was 1.4 ± 0.4 mm and 0.6 ± 0.3 deg, which was similar to UltraTrack (1.1 ± 0.3 mm, 0.5 ± 0.1 deg) and less than TimTrack (3.5 ± 1.0 mm, 1.4 ± 0.5 deg). UltraTimTrack was less affected by experimental conditions and image acquisition settings than its parent algorithms. It also yielded similar or lower root-mean-square deviations from manual tracking for previously published image sequences (fascicle length: 2.3–2.6 mm, fascicle angle: 0.8–0.9 deg) compared with a recently-proposed hybrid algorithm (4.7 mm, 0.9 deg), and the recently-proposed DL_Track algorithm (3.8 mm, 3.9 deg). Furthermore, UltraTimTrack’s processing time (0.2 s per image) was at least five times shorter than that of these recently-proposed algorithms. Conclusion We developed a Kalman-filter-based algorithm to improve fascicle tracking from B-mode ultrasound image sequences. The proposed algorithm provides low-noise, drift-free estimates of muscle architectural changes that may better inform muscle function interpretations.

Background: The aim of this study was to compare the plantar pressure distribution and knee and ankle muscle architecture in women with and without knee osteoarthritis (OA). Methods: Fifty women with knee OA (mean age = 52.11 ± 4.96 years, mean Body mass index (BMI) = 30.94 ± 4.23 kg/m2) and 50 healthy women as a control group (mean age = 50.93 ± 3.78 years, mean BMI = 29.06 ± 4.82 kg/m2) were included in the study. Ultrasonography was used to evaluate knee and ankle muscles architecture and femoral cartilage thickness. The plantar pressure distribution was evaluated using the Digital Biometry Scanning System and Milleri software (DIASU, Italy). Static foot posture was evaluated using the Foot Posture Index (FPI), and pain severity was assessed using the Visual Analog Scale. Results: The OA group exhibited lower muscle thickness in Rectus Femoris (RF) (p = 0.003), Vastus Medialis (VM) (p = 0.004), Vastus Lateralis (p = 0.023), and Peroneus Longus (p = 0.002), as well as lower Medial Gastrocnemius pennation angle (p = 0.049) and higher Fat thickness (FT) in RF (p = 0.033) and VM (p = 0.037) compared to the control group. The OA group showed thinner femoral cartilage thickness (p = 0.001) and higher pain severity (p = 0.001) than the control groups. FPI scores were higher (p = 0.001) in OA group compared to the control group. The plantar pressure distribution results indicated an increase in total surface (p = 0.027), total load (p = 0.002), medial load (p = 0.005), and lateral load (p = 0.002) on dominant side in OA group compared to the control group. Conclusions: Knee and ankle muscle architecture, knee extensor muscle FT, and plantar pressure distribution in the dominant foot differed in individuals with knee OA compared to the control group. [ABSTRACT FROM AUTHOR]

The shape of skeletal muscle varies remarkably—with important implications for locomotor performance. In many muscles, the fibres are arranged at an angle relative to the tendons’ line of action, termed the pennation angle. These pennate muscles allow more sarcomeres to be packed side by side, enabling the muscle to generate higher maximum forces for a given muscle size. Historically, the physiological cross-sectional area (PCSA) has been used to capture both the size and arrangement of muscle fibres, and is one of the best predictors of a muscles capacity to produce force. However, the anatomical and mechanical implications of PCSA remain ambiguous as misinterpretations have limited our ability to understand the mechanical advantage of pennate muscle designs. We developed geometric models to resolve the mechanistic and functional impacts of pennation angle across a range of muscle shapes and sizes. Comparisons among model predictions and empirical data on human lower limb muscles demonstrated how a pennate arrangement of fibres allows muscles to produce up to six times more isometric force when compared with non-pennate muscles of the same volume. We show that in muscles much longer than thick, an optimal pennation angle exists at which isometric force is maximized. Using empirically informed geometric models we demonstrate the functional significance of a pennate muscle design and provide a new parameter, pennation mechanical advantage, which quantifies this performance improvement.

This cross-sectional study examined gastrocnemius medialis fascicle length (FL) and anatomical cross-sectional area (ACSA) in female volleyball athletes aged 8–18 years, in an attempt to investigate morphological growth changes during childhood and adolescence. Methods: Eighty-nine athletes were assigned to five groups: 8–10, 10–12, 12–14, 14–16 and 16–18 years old. Height, body mass, leg and calf length were measured, and maturity offset was calculated from anthropometrics. FL and ACSA were assessed using ultrasonography. Results: FL was longer and ACSA was larger in the 12–14 y group compared with the 8–10 y group (4.31 ± 0.60 vs. 3.66 ± 0.40 cm, p = 0.008 and 13.08 ± 3.01 cm2 vs. 9.98 ± 2.07 cm2, p = 0.018, respectively). However, there were no differences in FL or ACSA among the groups aged between 12 and 18 years (p > 0.573 and p > 0.352). Notably the percentage increase in ACSA between the youngest (8–10 y) and the oldest group (16–18 y) was twofold greater than the percentage increase in FL (49% vs. 24%). Significant correlations were found between anthropometric characteristics FL and ACSA (r = 0.366–760, p < 0.001). Conclusions: These data indicate that there is a rapid increase in FL and ACSA from the age of 8 y until the age of 14 y, which is a crucial time frame for muscle growth.

Purpose: We investigated the effect of an unsupervised, body mass- home-based resistance training program in older adults performed at either a fast or slow contractile speed on changes to muscle-power, -volume, -architecture, and fatigue resistance of the knee extensors. Methods: Thirty-two male older adults (age 65–88 years) were separated into 1) fast-speed exercise (Fast-group), 2) slow-speed exercise (Slow-group), and 3) no exercise (Control-group) groups. Participants in the exercise groups performed 30–45 repetitions of knee-extension and sit-to-stand exercises 3 times a week for 8 weeks with different exercise speed between the groups. Before and after the intervention period, the following variables were measured: Isotonic power, isometric strength, twitch contractile properties, muscle-activity, -architecture, and -quality, neuromuscular fatigue resistance of the knee extensors, and thigh muscle volume. Results: Peak power was increased in both the Fast-group (+24 %, P

Background and objectiveFactors related to muscle architecture may lead to functional limitations in activities of daily living in the older adults. This study aimed to investigate the relationship between quadriceps femoris (QF) architecture and physical function in older adults community-dwelling people.MethodsThe study included 25 community-dwelling older adults participants aged over 60 years (14 women and 11 men) who were not engaged in regular physical activity. The rectus femoris (RF) and vastus intermedius (VI) muscle thicknesses as well as the RF cross-sectional area (CSA) were assessed using 2D ultrasonography. The 30 Seconds Chair Stand test (30sCST) and Timed Up and Go Test (TUG) were used to assess lower body muscle power and functional mobility, respectively.ResultsThe QF muscle architecture showed moderate and large correlations with the 30sCST (r range = 0.45–0.67, p

IntroductionParkinson's disease (PD) is a prevalent neurodegenerative condition observed primarily in the elderly population that gives rise to motor and non-motor symptoms, one of which is muscle weakness. The aim of this study was to characterize the vastus lateralis torque-fascicle length (T-L) and the knee extensors torque-angular velocity (T-V) and power-angular velocity (P-V) relationships in PD patients and to investigate the influence of muscle geometry on muscle mechanics.MethodsParticipants (11 PD: patients, 9 CR: age matched healthy controls; 10 CY: young healthy controls) performed: (i) isometric contractions (e.g., MVC) to obtain the torque-angle and T-L relationships; (ii) isokinetic (e.g., iso-velocity) contractions to obtain the T-V and P-V relationships. During the experiments, the architecture of vastus lateralis (pennation angle, fascicle length, muscle thickness) was also determined by using an ultrasound apparatus.ResultsSignificant differences were observed between PD patients and physically matched control groups (CR and CY) in terms of maximum isometric force (calculated as the apex of the T-L curve) and maximum mechanical power (apex of the P-V curve), but not in maximum shortening velocity. Among the mechanical variables investigated, mechanical power was able to identify differences between the less and the more affected side in PD patients, suggesting that this parameter could be useful for clinical evaluation in this population.ConclusionsThe observed results cannot be explained by differences in muscle geometry at rest (similar in the three cohorts), but rather by the muscle capacity to change in shape during contraction, that is impaired in PD patients.

Objective:This study aims to explore the effects of 12-week gait retraining (GR) on plantar flexion torque, architecture, and behavior of the medial gastrocnemius (MG) during maximal voluntary isometric contraction (MVIC).Methods:Thirty healthy male rearfoot strikers were randomly assigned to the GR group (n = 15) and the control (CON) group (n = 15). The GR group was instructed to wear minimalist shoes and run with a forefoot strike pattern for the 12-week GR (3 times per week), whereas the CON group wore their own running shoes and ran with their original foot strike pattern. Participants were required to share screenshots of running tracks each time to ensure training supervision. The architecture and behavior of MG, as well as ankle torque data, were collected before and after the intervention. The architecture of MG, including fascicle length (FL), pennation angle, and muscle thickness, was obtained by measuring muscle morphology at rest using an ultrasound device. Ankle torque data during plantar flexion MVIC were obtained using a dynamometer, from which peak torque and early rate of torque development (RTD50) were calculated. The fascicle behavior of MG was simultaneously captured using an ultrasound device to calculate fascicle shortening, fascicle rotation, and maximal fascicle shortening velocity (Vmax).Results:After 12-week GR, 1) the RTD50 increased significantly in the GR group (p = 0.038), 2) normalized FL increased significantly in the GR group (p = 0.003), and 3) Vmax increased significantly in the GR group (p = 0.018).Conclusion:Compared to running training, GR significantly enhanced the rapid strength development capacity and contraction velocity of the MG. This indicates the potential of GR as a strategy to improve muscle function and mechanical efficiency, particularly in enhancing the ability of MG to generate and transmit force as well as the rapid contraction capability. Further research is necessary to explore the effects of GR on MG behavior during running in vivo.

Introduction Lower limb muscle strength is an important predictor of sports performance, injury risk and frailty in ageing. The strength of a muscle is determined by its geometry and neuronal factors. Muscle geometry can be subdivided into architecture and morphology. Muscle morphology describes shape characteristics such as anatomical cross-sectional area (ACSA), thickness or volume (Maden-Wilkinson et al., 2021). Muscle architecture is determined by muscle fascicle length and the insertion angle of the muscle fascicles in the aponeuroses and describes the orientation of the muscle fibers relative to their force generation axis (Lieber & Friden, 2000). Muscle geometry is associated to physical performance and strength in humans (Maden-Wilkinson et al., 2021; Werkhausen et al., 2022) and is therefore a main research interest. A cost-effective and participant friendly method to validly and reliably assess muscle geometry is ultrasonography. However, a major limitation of ultrasonography is the subjectivity of image acquisition and the time-consuming image analysis (Ritsche et al., 2021; Ritsche, Wirth, et al., 2022; Ritsche et al., 2023). Moreover, image characteristics are massively influenced by the ultrasonography device used (Ritsche, Schmid, et al., 2022) as well as the muscle region scanned (Monte & Franchi, 2023). This poses constraints on the generalizability of existing automated image analysis approaches. The goal of this series of studies is therefore to optimize the ultrasonography acquisition and data analysis procedures by developing open-source software packages. Secondly, we aim to apply these methods in a sports performance context and describe the relevance of muscle geometry. Methods To streamline the time-consuming and subjective process of image analysis, we developed open-source and user-friendly software packages for muscle geometry analysis in lower limb muscles. We developed a semi-automated algorithm “ACSAuto” for assisted analysis of muscle ACSA using common image filtering processes (Ritsche et al., 2021). Given the limited generalizability and required user input of this approach, we developed two fully automated software applications, “DeepACSA” and “DL_Track_US”, using convolutional neural networks for more time efficient and robust analysis of lower limb muscle geometry (Ritsche et al., 2023; Ritsche, Wirth, et al., 2022; Ritsche et al., in press). We compared the predictions in an unseen test set to the current state-of-the-art, manual analysis, in order to evaluate the performance of our algorithms. To broaden the application of ultrasonography for evaluating muscle geometry in a sports context, we investigated the validity of a low-cost mobile ultrasonography device compared to a high-end counterpart in assessing various muscle architectural parameters in healthy adults (Ritsche, Schmid, et al., 2022).The mobile ultrasonography setup consisted of a smartphone and a portable probe, enabling practitioners high flexibility in the assessment of muscle architecture. We further investigated the link between muscle geometry and performance among soccer players. In one study, we focused on the m. biceps femoris long head in under-13 to under-15 youth players, assessing architecture and morphology at the mid-muscle point and correlating these with their sprint times and maximum velocity (Ritsche et al., 2020). In a further study, we analyzed the mm. vastus lateralis and rectus femoris in both youth and adult players of both sexes, evaluating muscle geometry at various muscle lengths alongside their knee extension strength during isometric and isokinetic conditions (Ritsche et al., in preparation and under review). Results Both ACSAuto and DeepACSA showed high comparability in assessing lower limb muscle ACSA with standard error of measurement lower than one cm2 (SEM ranging from 1.2 to 9.5%; Ritsche et al., 2021; Ritsche, Wirth, et al., 2022). Moreover, DeepACSA provided fast and objective analysis comparable to manual segmentation with no supervision of the analysis process needed. The time needed for analysis was reduced by a factor of 10. DL_Track_US demonstrated high comparability to manual muscle architecture analysis of images and videos, i.e. dynamic situations, (Ritsche et al., 2023; Ritsche et al., in press) and a reduction in the duration of analysis by a factor of 100. The mobile ultrasonography system showed a high degree of reliability and comparability only for m. gastrocnemius medialis architecture assessment, with a standard error of measurement lower than 10% for all architectural parameters (Ritsche, Schmid, et al., 2022). Thus, its reliability and comparability depended on the muscle assessed. We observed relevant correlations between muscle ACSA in young and adult male soccer players as well as in female soccer players and performance (Ritsche et al., 2020; Ritsche et al., unpublished). Moreover, we observed changes in muscle geometry with age and differences between males and females. Specifically, m. biceps femoris ACSA was strongly correlated with 30m sprint times and maximal velocity (r = -0.61 and r = 0.61, respectively), highlighting its importance in athletic performance (Ritsche et al., 2020). M. vastus lateralis ACSA at 50% of muscle length was most frequently related to knee extension strength (r = 0.40 - 0.53), which was observed in both sexes and across several age groups of male soccer players (Ritsche et al., in preparation and under review). Relevant correlations occurred more frequently in older age groups and higher knee extension velocities. Interestingly, we did not observe relevant correlations between muscle architecture and performance in the mm. biceps femoris and vastus lateralis. Discussion/Conclusion The results of this series studies so far led to three main insights. Firstly, the development of the “ACSAuto”, “DeepACSA” and “DL_Track_US” tools, utilizing semi-automated and fully automated analysis techniques applying deep learning algorithms, marked another step forward in overcoming the subjectivity and time consuming image evaluation. In a user-friendly way, these tools enable reproducible and objective analyses of muscle geometry in ultrasonography images. Secondly, with technological advancements, assessing muscle geometry with ultrasonography is possible using a smartphone and a probe, and often gives comparable results to high-end devices (Ritsche, Schmid, et al., 2022). This allows for a broader and more versatile application of muscle geometry assessment. However, our results highlight the need for a selective approach based on the muscle group being assessed and technical improvements of existing devices. Lastly, our findings across several investigations reveal a relevant positive correlation between muscle ACSA and performance metrics such as sprint times and knee extension strength (Ritsche et al., 2020; Ritsche et al., unpublished), corroborating previous research (Maden-Wilkinson et al., 2021; Monte & Franchi, 2023). The relationship was more pronounced in older age groups, suggesting that muscle geometry's influence on performance may amplify with athletic maturity. Apart from that, we observed the relationship in the m. vastus lateralis to be region- and contraction velocity-dependent. In agreement with Werkhausen et al. (2022), no relation of muscle architecture with strength when assessed in a static resting position was observed. This highlights the need for a potential shift towards assessing changes in muscle geometry during contraction rather than in static situations when evaluating the relation between muscle geometry and performance. Finally, remaining challenges include the comparability of muscle geometry assessment in the literature, the analysis methods used and the low generalizability of available automated analysis approaches (ours included). There is a clear need for methodological consensus on the assessment of muscle geometry when using ultrasonography, and more versatile analysis approaches are needed to enable an easy, generalizable and reproducible analysis of images and videos. Therefore, future works should target to establish assessment and analysis guidelines of muscle geometry in ultrasonography images to increase the comparability and reproducibility of results. Moreover, assessing changes in muscle geometry during contraction rather than during rest should be focused. References Lieber, R. L., & Friden, J. (2000). Functional and clinical significance of skeletal muscle architecture. Muscle Nerve, 23(11), 1647–1666. https://doi.org/10.1002/1097-4598(200011)23:11%3C1647::aid-mus1%3E3.0.co;2-m Maden-Wilkinson, T. M., Balshaw, T. G., Massey, G. J., & Folland, J. P. (2021). Muscle architecture and morphology as determinants of explosive strength. European Journal of Applied Physiology, 121(4), 1099–1110. https://doi.org/10.1007/s00421-020-04585-1 Monte, A., & Franchi, M. V. (2023). Regional muscle features and their association with knee extensors force production at a single joint angle. European Journal of Applied Physiology, 123, 2239-2248. https://doi.org/10.1007/s00421-023-05237-w Ritsche, P., Bernhard, T., Roth, R., Lichtenstein, E., Keller, M., Zingg, S., Franchi, M. V., & Faude, O. (2020). M. biceps femoris long head architecture and sprint ability in youth soccer players. International Journal of Sports Physiology and Performance, 16(11), 1616-1624. https://doi.org/10.1123/ijspp.2020-0726 Ritsche, P., Schmid, R., Franchi, M. V., & Faude, O. (2022). Agreement and reliability of lower limb muscle architecture measurements using a portable ultrasound device. Frontiers in Physiology, 13, Article 981862. https://doi.org/10.3389/fphys.2022.981862 Ritsche, P., Seynnes, O., & Cronin, N. (2023). DL_Track_US: A python package to analyse muscleultrasonography images. Journal of Open Source Software, 8(85), Article 5206. https://doi.org/10.21105/joss.05206 Ritsche, P., Wirth, P., Cronin, N. J., Sarto, F., Narici, M. V., Faude, O., & Franchi, M. V. (2022). DeepACSA: Automatic segmentation of cross-sectional area in ultrasound images of lower limb muscles using deep learning. Medicine & Science in Sports & Exercise, 54(12), 2188-2195. https://doi.org/10.1249/MSS.0000000000003010 Ritsche, P., Wirth, P., Franchi, M. V., & Faude, O. (2021). ACSAuto-semi-automatic assessment of human vastus lateralis and rectus femoris cross-sectional area in ultrasound images. Scientific Reports, 11, Article 13042. https://doi.org/10.1038/s41598-021-92387-6 Werkhausen, A., Gløersen, Ø., Nordez, A., Paulsen, G., Bojsen-Møller, J., & Seynnes, O. R. (2022). Rate of force development relationships to muscle architecture and contractile behavior in the human vastus lateralis. Scientific Reports, 12, Article 21816. https://doi.org/10.1038/s41598-022-26379-5

Background/aim: Isokinetic strength assessment of the rotator cuff muscle is frequently applied in a variety of shoulder postures, but none of these consider muscular architecture, which is one of the most important aspects of improving strength development. This study aimed to examine the test and retest reliability and validity of the muscle architecture-based position (MABP), which is 25° abduction and 20° external rotation, in healthy subjects to be able to select a better isokinetic assessment position for shoulder rotator cuff muscles. Materials and methods: A total of 54 healthy males with a mean age of 21.0 ± 1.2 years and mean body mass index of 22.8 ± 1.7 kg/m2 completed an isokinetic measurement session. All of the tests were performed on an IsoMed 2000 isokinetic dynamometer concentrically and eccentrically for both upper limbs at 60°/s angular velocity. All of the participants completed 3 measurement sessions: the first represented the isokinetic testing and was performed in the scapular neutral position (SNP) (45° shoulder flexion and abduction), the second represented the MABP (25° abduction and 20° ER) for shoulder rotator cuff muscles, and the third represented the test and retest of the MABP. Results: The correlations between the 2 techniques for assessing concurrent validity ranged from 0.908 to 0.994. The values obtained from the MABP were higher than those obtained in the SNP. There was no systematic bias for any measurements between the MABP and the retest of the MABP (p > 0.05). The intraclass correlation coefficients representing the test and retest reliability results for each variable measured with the MABP was higher than 0.98 and this value was considered as excellent reliability. Conclusion: In conclusion, the MABP can be used to assess the isokinetic strength of the rotator cuff muscles safely and confidently, with increased quantities of force being released and measurement at optimal muscle tension. [ABSTRACT FROM AUTHOR]

Background: Plantar intrinsic foot muscles (PIFMs) are composed of 10 muscles and play an essential role in achieving functional diversity in the foot. Previous studies have identified that the morphological profiles of PIFMs vary between individuals. The morphological profiles of a muscle theoretically reflect its output potentials: the physiological cross-sectional area (PCSA) of a muscle is proportional to its maximum force generation, and the muscle fiber length (FL) is its shortening velocity. This implies that the PCSA and FL may be useful variables for characterizing the functional diversity of the individual PIFM. The purpose of this study was to examine how individual PIFMs can be classified based on their PCSA and FL. Methods: In 26 healthy young adult males, the muscle volume and muscle length of seven PIFMs (abductor hallucis, ABDH; abductor digiti minimi, ABDM; adductor hallucis oblique head, ADDH-OH; ADDH transverse head, ADDH-TH; flexor digitorum brevis, FDB; flexor hallucis brevis, FHB; quadratus plantae, QP) were measured using magnetic resonance imaging. The PCSA and FL of each of the seven PIFMs were then estimated by combining the data measured from the participants and those of muscle architectural parameters documented from cadavers in previous studies. A total of 182 data samples (26 participants × 7 muscles) were classified into clusters using k-means cluster analysis. The optimal number of clusters was evaluated using the elbow method. Results: The data samples of PIFMs were assigned to four clusters with different morphological profiles: ADDH-OH and FHB, characterised by large PCSA and short FL (high force generation and slow shortening velocity potentials); ABDM and FDB, moderate PCSA and moderate FL (moderate force generation and moderate shortening velocity potentials); QP, moderate PCSA and long FL (moderate force generation and rapid shortening velocity potentials); ADDH-TH, small PCSA and moderate FL (low force generation and moderate shortening velocity potentials). ABDH components were assigned equivalently to the first and second clusters. Conclusions: The approach adopted in this study may provide a novel perspective for interpreting the PIFMs' function based on their maximal force generation and shortening velocity potentials. [ABSTRACT FROM AUTHOR]

The aim of this review was to provide an overview of the recent findings on the acute and chronic effects of static stretching on joint behaviors and neuromuscular responses and to discuss the overall effects of acute and chronic static stretching on selected outcomes via meta-analyses, using a total of 50 recent studies. The results of our meta-analyses demonstrated that acute static stretching results in increased range of motion (ROM), decreased passive resistive torque (PRT), increased maximum tolerable PRT (PRTmax), decreased maximum voluntary isometric torque, decreased muscle–tendon unit stiffness, decreased muscle stiffness, decreased tendon stiffness, and decreased shear elastic modulus. Moreover, the chronic effects of static stretching included increased ROM, increased PRTmax, decreased muscle stiffness, and decreased shear elastic modulus (or shear wave speed). These results suggest that static stretching interventions have the potential to increase ROM and reduce the mechanical properties of muscle–tendon tissue, but they may not change corticospinal excitability and spinal reflex excitability or muscle architecture parameters. [ABSTRACT FROM AUTHOR]

Abstract Background In endurance running, elite Kenyan runners are characterized by longer thigh, shank, and Achilles tendon (AT) lengths combined with shorter fascicles and larger medial gastrocnemius (MG) pennation angles than elite Japanese runners. These muscle-tendon characteristics may contribute to the running performance of Kenyans. Furthermore, these specific lower-leg musculoskeletal architectures have been confirmed not only in elite Kenyan runners but also in non-athletic Kenyans since early childhood. However, it remains questionable whether the differences in muscle-tendon architecture between Kenyans and Japanese differ from those of European Caucasians. Therefore, this study aimed to compare anthropometry and muscle–tendon architecture of young non-athletic Kenyan males with their Japanese and French counterparts. Methods A total of 235 young non-athletic males, aged 17–22 years, volunteered. The anthropometric measures, thigh, and shank lengths, as well as AT and MG muscle architecture, were measured using ultrasonography and a tape measure. Inter-group differences in anthropometry and muscle-tendon architecture were tested using one-way ANOVA and ANCOVA analyses controlling for shank length and muscle thickness. Results The anthropometric and muscle-tendon characteristics of the non-athletic French were closer to those of the Kenyans than to those of the Japanese. However, the ultrasonography analysis confirmed that the non-athletic Kenyans had the longest AT as well as the shortest MG fascicles and the largest pennation angle compared to the French and Japanese, even after controlling for shank length and muscle thickness with ANCOVA, respectively. Conclusions These results confirmed the specificity of the muscle-tendon architecture of the triceps surae in Kenyans in comparison to their Japanese and French counterparts in non-athletic adults. This study provides additional support to the fact that Kenyans may have musculotendinous advantages in endurance running.

The aim of this study was to compare muscle architecture and performance between male and female elite Field Hockey players and to investigate the relationships between echo intensity and performance. Twenty-one male (24.3 ± 3.6 y; 75.1 ± 8.5 kg; 176.8 ± 6.4 cm) and nineteen female players (27.4 ± 3.9 y; 61.2 ± 7.4 kg; 164.4 ± 4.9 cm) were tested for muscle thickness (MT) and echo intensity (EI) of trapezius (Trap) and vastus lateralis (VL) muscles. Participants were also assessed for bench press power, and 30 m sprint. Results showed a higher VLMT and TrapMT in male players compared to female players (+22.1%; p = 0.004 and +25.8%; p = 0.001 for VLMT and TrapMT, respectively). A lower VLEI was detected in male players compared to female players (−20.7%; p = 0.001), while no significant differences were detected for TrapEI. Male players were faster than female players in a 30 m sprint and more powerful at the bench press (p < 0.001). Significant correlations were detected between VLEI and 30 m sprint (r = 0.74) in female players only. Results indicate that differences exist between male and female elite Field Hockey players in the EI of lower body muscles, while these differences are not present in the upper body muscles. EI, together with other sprint and power assessments, may represent an important parameter for elite Field Hockey players. [ABSTRACT FROM AUTHOR]

Simple Summary: Ageing sometimes leads to changes in the size, shape and structure of muscles. This impacts their ability to perform their functions and so leads to an increased likelihood of injuries and negatively impacts quality of life. This work attempts to investigate how the structure and performance of individual muscles of the human leg may change due to ageing, using a combination of magnetic resonance imaging and muscle strength testing in groups of aged (65+ years old) and young (18–40 years old) individuals. The results showed a larger difference in muscle structure and strength in the muscles that extend the knee (i.e., the quadriceps) between the age groups relative to other muscles, and these muscles also showed the strongest relationships between structure and strength. There was also greater variation in fibre lengths and variables linked to fibre type and quality within individual muscles in the aged group compared to the young group. Overall, these results show that even healthy ageing may cause large changes in muscle structure and decreases in performance and that the quadriceps muscles could be a target for exercises to reduce the negative effects of ageing. Even "healthy" muscle ageing is often associated with substantial changes in muscle form and function and can lead to increased injury risks and significant negative impacts on quality of life. However, the impacts of healthy muscle ageing on the fibre architecture and microstructure of different muscles and muscle groups throughout the lower limb, and how these are related to their functional capabilities, are not fully understood. Here, a previously established framework of magnetic resonance and diffusion tensor imaging was used to measure the muscle volumes, intramuscular fat, fibre lengths and physiological cross-sectional areas of 12 lower limb muscles in a cohort of healthily aged individuals, which were compared to the same data from a young population. Maximum muscle forces were also measured from an isokinetic dynamometer. The more substantial interpopulation differences in architecture and functional performance were located within the knee extensor muscles, while the aged muscles were also more heterogeneous in muscle fibre type and atrophy. The relationships between architecture and muscle strength were also more significant in the knee extensors compared to other functional groups. These data highlight the importance of the knee extensors as a potential focus for interventions to negate the impacts of muscle ageing. [ABSTRACT FROM AUTHOR]

Purpose: The present study compared the effects of contraction intensity (submaximal vs maximal) and mode (concentric vs eccentric) on biceps femoris long head (BFlh) fascicle lengthening, rotation, and architectural gear ratio at long and short muscle lengths. Methods: Data were captured from 18 healthy adults (10 men and 8 women) without history of right hamstring strain injury. BFlh fascicle length (Lf), fascicle angle (FA), and muscle thickness (MT) were assessed in real time using two serially aligned ultrasound devices while submaximal and maximal concentric and eccentric isokinetic knee flexions were performed at 30°·s-1. Ultrasound videos were exported and edited to create a single, synchronized video, and three fascicles were analyzed through the range of motion (10° to 80°). Changes (Δ) in Lf, FA, MT, and muscle gear at long (60° to 80° knee angle; 0° = full knee extension) and short (10° to 30°) muscle lengths and across the full knee flexion range were measured and compared. Results: Greater ΔLf was observed at long muscle length (P < 0.001) during both submaximal and maximal eccentric and concentric contractions. When the full length range was analyzed, a slightly greater ΔMT was observed in concentric contractions (P = 0.03). No significant differences between submaximal and maximal contractions were observed for ΔLf, ΔFA, or ΔMT. No changes were detected in the calculated muscle gear between muscle lengths, intensities, or conditions (P > 0.05). Conclusions: Although gear ratio ranged ~1.0 to 1.1 undermost conditions, the increased fascicle lengthening observed at long muscle lengths might influence acute myofiber damage risk but also speculatively play a role in chronic hypertrophic responses to training. [ABSTRACT FROM AUTHOR]

Goal: Skeletal muscle mechanics can be assessed in vivo using shear wave elastography. However, the impact of pennation angle on shear wave velocity (SWV) remains unclear. This study aims to quantify the effect by automatically aligning the ultrasound probe with muscle fiber orientation. Methods: We propose an automatic ultrasound probe alignment system and compare it to manual and no alignment. SWV of the gastrocnemius medialis muscle of ten volunteers was measured during rest and isometric contractions. Results: The SWV was different between the conditions (p = 0.008). The highest SWV was obtained during the automatic alignment and differences between the conditions were most pronounced during high-level contractions. The automatic system yielded more accurate alignment compared to a manual operator (p = 0.05). Conclusions: The present study indicates that pennation angle affects SWV, hence muscle fiber orientation must be considered to reliably interpret SWV. Using automatic alignment systems allows for more accurate alignment, improving the methodology of ultrasound elastography in skeletal muscles.

Background: Knowledge of the muscle's lengths at which maximum active isometric force is attained is important for predicting forces during movement. However, there is limited information about the in vivo force-length properties of a human muscle that plays crucial roles during locomotion; the tibialis anterior (TA). We therefore aimed to estimate TA's force-length relation from dorsiflexor torque-angle curves constructed from eight women and eight men. Methods: Participants performed maximal voluntary fixed-end contractions with their right ankle dorsiflexors from 0° to 30° plantar flexion. Muscle fascicle lengths were estimated from B-mode ultrasound images, and net ankle joint torques were measured using dynamometry. Fascicle forces were estimated by dividing maximal active torques by literature-derived, angle-specific tendon moment arm lengths while assuming a fixed 50% force contribution of TA to the total dorsiflexor force and accounting for fascicle angles. Results: Maximal active torques were higher at 15° than 20° and 30° plantar flexion (2.4-6.4 Nm, p ≤ 0.012), whereas maximal active TA fascicle forces were higher at 15° than 0°, 20° and 30° plantar flexion (25-61 N, p ≤ 0.042), but not different between 15° and 10° plantar flexion (15 N, p = 0.277). TA fascicle shortening magnitudes during fixed-end contractions were larger at 15° than 30° plantar flexion (3.9 mm, p = 0.012), but less at 15° than 0° plantar flexion (-2.4 mm, p = 0.001), with no significant differences (≤0.7 mm, p = 0.871) between TA's superficial and deep muscle compartments. Series elastic element stiffness was lowest and highest at lengths 5% shorter and 5% longer than optimum fascicle length, respectively (-30 and 15 N/mm, p ≤ 0.003). Discussion: TA produced its maximum active force at 10-15° plantar flexion, and its normalized force-length relation had ascending and descending limbs that agreed with a simple scaled sarcomere model when active fascicle lengths from within TA's superficial or deep muscle compartment were considered. These findings can be used to inform the properties of the contractile and series elastic elements of Hill-type muscle models. [ABSTRACT FROM AUTHOR]

The aim of this study was to compare the effects of sit-to-stand (STS) training programs with 5 vs. 10 repetitions on muscle architecture and muscle function in sedentary adults. Sixty participants were randomly assigned into three groups: five-repetition STS (5STS), 10-repetition STS (10STS), or a control group (CG). Participants performed three sets of five or 10 repetitions of the STS exercise three times per week for 8 weeks. Before and after 8 weeks, all groups performed ultrasound measures to evaluate muscle thickness (MT), pennation angle (PA), and fascicle length (FL), and the five-repetition STS test to estimate the relative STS power and muscle quality index (MQI). After 8 weeks, both experimental groups improved MQI (40-45%), relative STS power (29-38%), and MT (8-9%) (all p < 0.001; no differences between the 5STS vs. 10STS groups). These improvements in both groups resulted in differences regarding the CG, which did not present any change. In addition, only the 5STS group improved PA (15%; p = 0.008) without differences to the 10STS and CG. This suggests that STS training is time-effective and low-cost for improving muscle function and generating adaptations in muscle architecture. [ABSTRACT FROM AUTHOR]

The aim of the present study was to analyse the role of exercises' resistance profile in regional hypertrophy. Thirty-eight healthy women completed a 9-week resistance training program consisting of either 4 sets of 12 repetitions to volitional failure of inclined bicep curls (INC group) or preacher curls (PREA group), three times per week. Pre- and post-intervention muscle thickness was measured using B-mode ultrasound imaging with a linear-array transducer. Scan acquisition sites were determined by measuring 50%, 60% and 70% of the distance between the posterior crest of the acromion and the olecranon. Statistical significance was set at p < 0.05. No region of the INC group grew when comparing pre- to post-intervention. The 70% region of the PREA group grew significantly (muscle thickness increased from 2.7 ± 0.43 cm to 2.94 ± 0.44 cm). We found no growth differences between regions when analysing per group (p = 0.274), region (p = 0.571) or group*region (p = 0.367). Our results show that the distal region of the arm grows in response to the preacher curl that places the highest amount of strain in the range of motion in which the arm muscles are more elongated. [ABSTRACT FROM AUTHOR]

Background: In endurance running, elite Kenyan runners are characterized by longer thigh, shank, and Achilles tendon (AT) lengths combined with shorter fascicles and larger medial gastrocnemius (MG) pennation angles than elite Japanese runners. These muscle-tendon characteristics may contribute to the running performance of Kenyans. Furthermore, these specific lower-leg musculoskeletal architectures have been confirmed not only in elite Kenyan runners but also in non-athletic Kenyans since early childhood. However, it remains questionable whether the differences in muscle-tendon architecture between Kenyans and Japanese differ from those of European Caucasians. Therefore, this study aimed to compare anthropometry and muscle–tendon architecture of young non-athletic Kenyan males with their Japanese and French counterparts. Methods: A total of 235 young non-athletic males, aged 17–22 years, volunteered. The anthropometric measures, thigh, and shank lengths, as well as AT and MG muscle architecture, were measured using ultrasonography and a tape measure. Inter-group differences in anthropometry and muscle-tendon architecture were tested using one-way ANOVA and ANCOVA analyses controlling for shank length and muscle thickness. Results: The anthropometric and muscle-tendon characteristics of the non-athletic French were closer to those of the Kenyans than to those of the Japanese. However, the ultrasonography analysis confirmed that the non-athletic Kenyans had the longest AT as well as the shortest MG fascicles and the largest pennation angle compared to the French and Japanese, even after controlling for shank length and muscle thickness with ANCOVA, respectively. Conclusions: These results confirmed the specificity of the muscle-tendon architecture of the triceps surae in Kenyans in comparison to their Japanese and French counterparts in non-athletic adults. This study provides additional support to the fact that Kenyans may have musculotendinous advantages in endurance running. [ABSTRACT FROM AUTHOR]

Skeletal muscle's isometric contractile properties are one of the classic structure–function relationships in all of biology allowing for extrapolation of single fibre mechanical properties to whole muscle properties based on the muscle's optimal fibre length and physiological cross‐sectional area (PCSA). However, this relationship has only been validated in small animals and then extrapolated to human muscles, which are much larger in terms of length and PCSA. The present study aimed to measure directly the in situ properties and function of the human gracilis muscle to validate this relationship. We leveraged a unique surgical technique in which a human gracilis muscle is transferred from the thigh to the arm, restoring elbow flexion after brachial plexus injury. During this surgery, we directly measured subject specific gracilis muscle force–length relationship in situ and properties ex vivo. Each subject's optimal fibre length was calculated from their muscle's length‐tension properties. Each subject's PCSA was calculated from their muscle volume and optimal fibre length. From these experimental data, we established a human muscle fibre‐specific tension of 171 kPa. We also determined that average gracilis optimal fibre length is 12.9 cm. Using this subject‐specific fibre length, we observed an excellent fit between experimental and theorical active length‐tension curves. However, these fibre lengths were about half of the previously reported optimal fascicle lengths of 23 cm. Thus, the long gracilis muscle appears to be composed of relatively short fibres acting in parallel that may not have been appreciated based on traditional anatomical methods. Key points: Skeletal muscle's isometric contractile properties represent one of the classic structure–function relationships in all of biology and allow scaling single fibre mechanical properties to whole muscle properties based on the muscle's architecture.This physiological relationship has only been validated in small animals but is often extrapolated to human muscles, which are orders of magnitude larger.We leverage a unique surgical technique in which a human gracilis muscle is transplanted from the thigh to the arm to restore elbow flexion after brachial plexus injury, aiming to directly measure muscles properties in situ and test directly the architectural scaling predictions.Using these direct measurements, we establish human muscle fibre‐specific tension of ∼170 kPa.Furthermore, we show that the gracilis muscle actually functions as a muscle with relatively short fibres acting in parallel vs. long fibres as previously assumed based on traditional anatomical models. [ABSTRACT FROM AUTHOR]

The aim of this study was to compare the effects of sit-to-stand (STS) training programs with 5 vs. 10 repetitions on muscle architecture and muscle function in sedentary adults. Sixty participants were randomly assigned into three groups: five-repetition STS (5STS), 10-repetition STS (10STS), or a control group (CG). Participants performed three sets of five or 10 repetitions of the STS exercise three times per week for 8 weeks. Before and after 8 weeks, all groups performed ultrasound measures to evaluate muscle thickness (MT), pennation angle (PA), and fascicle length (FL), and the five-repetition STS test to estimate the relative STS power and muscle quality index (MQI). After 8 weeks, both experimental groups improved MQI (40–45%), relative STS power (29–38%), and MT (8–9%) (all p < 0.001; no differences between the 5STS vs. 10STS groups). These improvements in both groups resulted in differences regarding the CG, which did not present any change. In addition, only the 5STS group improved PA (15%; p = 0.008) without differences to the 10STS and CG.This suggests that STS training is time-effective and low-cost for improving muscle function and generating adaptations in muscle architecture.

Skeletal muscles play a fundamental role in people's lives and their evaluation provides significant information on health. Different tools have been used to evaluate muscle mass, and the evaluation of muscle thickness (MT) using ultrasound has been included as an alternative, which can be performed with the probe in different positions; however, these could present differences. The objectives of this study were to determine whether there are differences in the measurement of MT in the vastus lateralis (VL) muscle using the probe in the longitudinal or transverse position, and to determine its association with the lean mass of the lower limbs. The results indicated no significant differences between MT measurements with the probe in the longitudinal and transverse positions (p =0.084). However, when associating these measurements with lower limb lean mass, it was found that transverse measurements had a strong association (r =0.547; p < 0.001), while longitudinal measurements had a moderate association (r =0.351; p =0.007). This suggests that measurements with the probe positioned transversely to measure the MT would be the best option. Therefore, it could be useful as an indicator of lower limb lean mass in the absence of tools, such as bioelectrical bioimpedance or magnetic nuclear resonance. [ABSTRACT FROM AUTHOR]

Objectives: This study aims to investigate skeletal muscle architecture and strength in patients with primary Sjögren syndrome (pSS). Patients and methods: Between July 01, 2017 and November 30, 2017, 19 pSS patients (19 females; mean age: 54.1±6.6 years; range, 42 to 62 years) and 19 age-, body mass index-, and sex-matched healthy controls (19 females; mean age: 53.2±6.7 years; range 42 to 61 years) were included. Sjögren symptoms were assessed with the European Alliance of Associations for Rheumatology (EULAR) Sjögren’s Syndrome Patient Reported Index (ESSPRI). Muscle thickness, pennation angle, and fascicle length were measured at quadriceps femoralis, gastrocnemius and soleus muscles. Isokinetic muscle strength tests were performed at 60 and 180°/sec for knee and at 30 and 120°/sec for ankle. Anxiety and depression evaluated with the Hospital Anxiety and Depression Scale (HADS), fatigue with Multidimensional Assessment of Fatigue scale (MAF), and functionality with Health Assessment Questionnaire (HAQ). Results: In the pSS group, the mean ESSPRI was 7.70±1.17. The mean scores of depression (10.05±3.09 vs. 4.47±2.29; p<0.0001), anxiety (8.26±4.28 vs. 3.79±2.42; p<0.0001), functionality (0.94±0.78 vs. 0.22±0.26; p<0.0001), and fatigue (37.69±5.47 vs. 17.69±5.26; p<0.0001) were significantly higher in patients with pSS. Only, the pennation angle of vastus medialis in dominant leg was significantly greater in healthy controls (p=0.049). Peak torques/body weight of knee and ankle muscles were found to be similar. Conclusion: Excluding a minor decrease of the pennation angle at vastus medialis, muscle structure of lower extremity of pSS patients were similar to healthy controls. In addition, isokinetic muscle strength did not significantly differ in patients with pSS compared to healthy controls. In patients with pSS, disease activity and fatigue level were negatively correlated with isokinetic muscle strength measurements. [ABSTRACT FROM AUTHOR]

When spinal stabilization exercises (SSE) are performed regularly, may provide benefits on outcome measures in chronic nonspecific neck pain (CNNP) patients. The pandemic has made it difficult for CNNP patients to access regular physiotherapy-exercise services. This study aims to compare telerehabilitation (TR) with face-to-face rehabilitation in CNNP. Neck Functional Capacity Evaluation Test (NFCET) results were the primary outcomes. Pain intensity (PI), disability, awareness, neck muscles architecture, andexercise satisfaction were the secondary outcomes. Patients were randomized into the TR group (TRG) (n = 15) and the control group (CG) (n = 16). Patients performed SSE 3 days a week, for 8 weeks. The TRG was instructed remotely while the CG was instructed in the clinic. After 8 weeks in both groups, NFCET values and neck awareness increased (p < 0.05), PI and disability decreased (p < 0.05). Muscle architecture improved in both groups (p < 0.05), except for the Right Sternocleidomastoideus in both groups and the Right Upper-Trapezius in TRG (p > 0.05). There was no difference between the groups for all variables and exercise satisfaction(p > 0.05). SSE for CNNP, whether supervised by therapists in the clinic or by telerehabilitation, was equally effective. NCT04691024 [ABSTRACT FROM AUTHOR]

In the field of wearable robotics, assistance needs to be individualized for the user to maximize benefit. Information from muscle fascicles automatically recorded from brightness mode (B-mode) ultrasound has been used to design assistance profiles that are proportional to the estimated muscle force of young individuals. There is also a desire to develop similar strategies for older adults who may have age-altered physiology. This study introduces and validates a ResNet + 2x-LSTM model for extracting fascicle lengths in young and older adults. The labeling was generated in a semimanual manner for young (40,696 frames) and older adults (34,262 frames) depicting B-mode imaging of the medial gastrocnemius. First, the model was trained on young and tested on both young (R2 = 0.85, RMSE = 2.36 ± 1.51 mm, MAPE = 3.6%, aaDF = 0.48 ± 1.1 mm) and older adults (R2 = 0.53, RMSE = 4.7 ± 2.51 mm, MAPE = 5.19%, aaDF = 1.9 ± 1.39 mm). Then, the performances were trained across all ages (R2 = 0.79, RMSE = 3.95 ± 2.51 mm, MAPE = 4.5%, aaDF = 0.67 ± 1.8 mm). Although age-related muscle loss affects the error of the tracking methodology compared to the young population, the absolute percentage error for individual fascicles leads to a small variation of 3–5%, suggesting that the error may be acceptable in the generation of assistive force profiles. [ABSTRACT FROM AUTHOR]

Abstract Background The aim of this study is to compare muscle strength and architecture between type 1 diabetes patients and healthy volunteers and to assess whether there is an ultrasonographic structural change in this population. Methods Thirty-two patients with T1D (23 female, 9 male) with an age average of 31.3 ± 8.7 years, matched in terms of age, gender, height, weight and physical activity were included in the study. In the T1D and control group, ultrasonographic measurements of quadriceps femoris muscle (RF, VI, VM, VL) and pennate angle (VI, VM, VL) were performed. Muscle strength values were measured using isokinetic dynamometer system at angular velocities of 60º/s and 180º/s in both groups. Results Initially, both groups were similar in demographic and clinical characteristics (p > 0.05). In the T1D group, there was a statistically significant difference in flexion/extension peak torque measurements at an angular velocity of 60º/s compared to the control group (p 0.05). When the T1D group was subgrouped, in the group that used insulin pump RF, VI, VM muscle thickness measurements were significantly higher (p

Abstract Background There is a lack of of cross-sectional research that has investigated muscle morphology, function, and functional capability in all age-bands of healthy adults. The primary aim of this study was to evaluate age-related differences in indices of vastus lateralis (VL) muscle morphology, function and functional capability in a sample of healthy males and females aged 18-70yrs. Secondary aims were to evaluate relationships between age and VL muscle morphology and function and functional capability. Methods B mode Ultrasonography and Tensiomyography were used to measure VL muscle thickness, pennation angle, fascicle length, and contractile properties in 274 healthy adults aged 18-70yrs. Measurements of grip strength and functional capability (1-min chair rise test) were also taken. Data analysis included descriptive statistics, correlations, one-way ANOVAs, and multiple regressions. Results Negative correlations were found between age and muscle thickness (rs = -.56), pennation angle (rs = -.50), fascicle length (rs = -.30), maximal displacement (rs = -.24), grip strength (rs = -.27) and the 1-min chair rise test (rs = -.32). Positive correlations were observed between age and the echo intensity of the muscle (rs = .40) and total contraction time (rs = .20). Differences in the indices of muscle health were noticeable between the 18–29 age band and the 50–59 and 60–70 age bands (p

The aim of this review was to provide an overview of the recent findings on the acute and chronic effects of static stretching on joint behaviors and neuromuscular responses and to discuss the overall effects of acute and chronic static stretching on selected outcomes via meta-analyses, using a total of 50 recent studies. The results of our meta-analyses demonstrated that acute static stretching results in increased range of motion (ROM), decreased passive resistive torque (PRT), increased maximum tolerable PRT (PRTmax), decreased maximum voluntary isometric torque, decreased muscle–tendon unit stiffness, decreased muscle stiffness, decreased tendon stiffness, and decreased shear elastic modulus. Moreover, the chronic effects of static stretching included increased ROM, increased PRTmax, decreased muscle stiffness, and decreased shear elastic modulus (or shear wave speed). These results suggest that static stretching interventions have the potential to increase ROM and reduce the mechanical properties of muscle–tendon tissue, but they may not change corticospinal excitability and spinal reflex excitability or muscle architecture parameters.

Abstract Background Age-related changes in muscle properties affect daily functioning, therefore a reliable assessment of such properties is required. We examined the effects of age on reliability, muscle quality and interrelation among muscle architecture (MA) parameters of the gastrocnemius medialis (GM), tibialis anterior (TA), and vastus lateralis (VL) muscles. Methods Three raters scored ultrasound (US) scans of 12 healthy younger and older adults, on fascicle length (FL), pennation angle (PA) and muscle thickness (MT). Intra- and inter-rater reliability of MA measures in rest and contraction was assessed by intraclass correlation coefficients (ICC) and standard error of measurements (SEM, SEM%). The relationship between MA parameters was examined using Pearson correlation coefficients. Muscle quality (MQ) was examined using mean pixel intensity. Results Reliability was moderate to excellent for TA in both groups (ICCs: 0.64–0.99, SEM% = 1.6–14.8%), and for VL in the younger group (ICCs: 0.67–0.98, SEM% = 2.0–18.3%). VL reliability was poor to excellent in older adults (ICCs: 0.22–0.99, SEM% = 2.7–36.0%). For GM, ICCs were good to excellent (ICCs: 0.76–0.99) in both groups, but GM SEM% were higher in older adults (SEM%Younger = 1.5–10.7%, SEM%Older = 1.6–28.1%). Muscle quality was on average 19.0% lower in older vs. younger adults. In both groups, moderate to strong correlations were found for VL FL and MT (r ≥ 0.54), and TA PA and MT (r ≥ 0.72), while TA FL correlated with MT (r ≥ 0.67) in younger adults only. Conclusions In conclusion, age- and muscle-specificities were present in the relationships between MT and PA, and MT and FL at rest. Furthermore, the reliability of MA parameters assessed with 2D panoramic US is acceptable. However, the level of reliability varies with age, muscle and MA measure. In older adults notably, the lowest reliability was observed in the VL muscle. Among the MA parameters, MT appears to be the simplest and most easily reproducible parameter in all muscles and age groups.

B-mode ultrasound is commonly used to image musculoskeletal tissues, but one major bottleneck is data analysis. Manual analysis is commonly deployed for assessment of muscle thickness, pennation angle and fascicle length in muscle ultrasonography images. However, manual analysis is somewhat subjective, laborious and requires thorough experience. We provide an openly available algorithm (DL_Track) to automatically analyze muscle architectural parameters in ultrasonography images or videos of human lower limb muscles. We trained two different neural networks (classic U-net [Ronneberger et al., 2021] and U-net with VGG16 [Simonyan & Zisserman, 2015] pretrained encoder) one to detect muscle fascicles and another to detect muscle aponeuroses using a set of labelled musculoskeletal ultrasound images. We included images from four different devices of the vastus lateralis, gastrocnemius medialis, tibilias anterior and soleus. In total, we included 310 images for the fascicle model and 570 images for the aponeuroses model, which we augmented to about 1,700 images per set. Each dataset was randomly split into a training and test set for model training, using a common 80/20 train/test split. We determined the best performing model based on intersection-over-union and loss metrics calculated during model training. We compared neural network predictions on an unseen test set consisting of 35 images to those obtained via manual analysis and two existing semi/automated analysis approaches (SMA and Ultratrack). Across the set of 35 unseen images, the mean differences between DL_Track and manual analysis were for fascicle length -2.4 mm (95% compatibility interval (CI) = -3.7 to -1.2), for pennation angle 0.6° (-0.2 to 1.4), and for muscle thickness -0.6 mm (-1.2 to 0.002). The corresponding values comparing DL_Track with SMA were for fascicle length 5.2 mm (1.3 to 9.0), for pennation angle -1.4° (-2.6 to -0.4) and for muscle thickness -0.9 mm (-1.5 to -0.3) respectively. ICC values between DL_Track and Ultratrack were 0.19 (0.00 to 0.35) for medial gastrocnemius passive contraction, 0.79 (0.77 to 0.81) for medial gastrocnemius maximal voluntary contraction, 0.88 (0.87 to 0.89) for calf raise, 0.67 (0.07 to 0.86) for medial gastrocnemius during walking, 0.80 (0.79 to 0.82) for tibialis passive plantar and dorsiflexion, and 0.85 (0.83 to 0.86) for tibialis anterior maximum voluntary contraction. Our method is fully automated and can estimate fascicle length, pennation angle and muscle thickness from single images or videos in multiple superficial muscles. For single images, the method gave results that are in agreement with those produced by SMA or manual analysis. Similarly, for videos, there was overlap between the results produced with Ultratrack and our method. In contrast to Ultratrack, DL_Track analyzes each frame independently of the previous frames, which might explain the observerd variability. References Ronneberger, O., Fischer, P., & Brox, T. (2021). U-Net: Convolutional networks for biomedical image segmentation. arXiv. https://doi.org/10.48550/arXiv.1505.04597 Simonyan, K., & Zisserman, A. (2015). Very deep convolutional networks for large-scale image recognition. arXiv. https://doi.org/10.48550/arXiv.1409.1556

Although ultrasonography is commonly used to assess muscle architecture, high-end ultrasonography devices lack in portability and are expensive. Recently, ultrasonography devices consisting solely of a probe, an app, and/or a mobile display were introduced. These devices have lower acquisition costs than high-end devices and demonstrate higher portability. Portable systems would allow for cost-effective, on-site athlete or patient screenings of muscle architecture as well as diagnosis of pathologies or injury. Furthermore, portable systems can be easily transported to allow for uncomplicated assessment of athletes belonging to different teams or patients in different hospital units. Nonetheless, the reliability of portable systems and the comparability to high-end devices for muscle architecture assessment have not been investigated. We investigated the agreement and reliability of a portable and handheld ultrasonography system for muscle architecture measurements in human lower limb muscles. We acquired images of the rectus femoris (RF), vastus lateralis (VL) and gastrocnemius medialis (GM) in 36 active healthy participants (15 female, 21 male) at 50% of muscle length using the handheld Lumify (L12-4, linear array 37 mm, Philips Healthcare, Amsterdam, Netherlands) and a high-end laboratory device (ACUSON Juniper, linear-array 54 mm, 12L3, SIEMENS Healthineers, Erlangen, Germany). Muscle length was determined as distance between the proximal and distal muscle tendon junction. We compared measurements of muscle fascicle length, pennation angle and thickness. To assess inter-session reliability of the Lumify system, participants were measured twice within 1 week. Comparing RF architecture measurements of both devices resulted in intra-class correlations (ICCs) ranging from 0.46–0.82 and standardized mean difference (SMDs) ranging from −0.45–0.05. For VL, ICCs ranged from 0.60–0.89 and SMDs ranged from −0.11–0.13. ICCs and SMDs for the GM ranged from 0.82–0.86 and −0.07–0.07. Calculating inter-session reliability for RF resulted in ICCs ranging from 0.44–0.76 and SMDs ranging from −0.38–0.15. For VL, ICCs and SMDs ranged from 0.57–0.75 and −0.13–0.02. ICCs for GM ranged from 0.75–0.92 and SMDs ranged from −0.15–0.16. The highest agreement (ICC ≥ 0.82) and reliability (ICC ≥ 0.75) across all muscles was demonstrated for the measurement of muscle thickness. The Lumify system was comparable to a high-end device and reliable for GM measurements. Nevertheless, agreement and reliability were lower for the RF and VL. Thus, when interpreting longitudinal observed changes in muscle architecture assessed with the Lumify system, measurement errors should be considered. Future investigations should consider including different participant populations, comparing the reliability of different portable systems for muscle architecture assessment as well as including different muscle groups.