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The exchange of technologies used in field cultivation for hydroponic systems can potentially increase plant development and grain production, requiring studies to verify the best management forms, such as growth-promoting bacteria and biostimulant compounds. With this in mind, the study aimed to evaluate the effect of the application of thiamine and niacin, alone and combined, to soybean plants in the absence and presence of inoculation with B. japonicum on the agronomic and physiological characteristics of the crop grown in an ebb and flow hydroponic system. Eight treatments were evaluated using t -test (LSD) and Tukey's test, both at 5% probability ( P < 0.05), in addition to Pearson correlation and canonical variables. The treatments consist of inoculation with B. japonicum at 1 mL 500 g -1 seeds (with and without) and foliar application of four solutions (water, niacin (0.1 g·L -1 ), thiamine (0.1 g·L -1 ), and niacin + thiamine (0.05 g·L -1  + 0.05 g·L -1 )). We found that inoculation significantly improved the parameters evaluated and resulted in a gain of approximately 84.8% in yield when compared by t -test ( P < 0.05). In addition, the action of the vitamins was more significant when they were applied without the presence of B. japonicum , especially niacin, either alone or combined with thiamine, which increased yield parameters in this condition, identified when the Tukey's test ( P < 0.05) was applied. We conclude that inoculation with Bradyrhizobium japonicum in soybean seeds grown in a hydroponic system significantly benefits the development and grain yield, mainly when combined with vitamin solutions. Niacin also has the potential to be used alone or combined with thiamine in noninoculated or inoculated hydroponic soybean crops, respectively., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2024 Alexandre H. de F. Lima et al.)

The objective of this study was to evaluate the genetic variability of natural rubber latex traits among 44 elite genotypes of the rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Müell. Arg.]. Multivariate analysis and machine learning techniques were used, targeting the selection of parents that demonstrate superior characters. We analyzed traits related to technological or physicochemical properties of natural rubber latex, such as Wallace plasticity (P 0 ), the plasticity retention index [PRI (%)], Mooney viscosity (V R ), ash percentage (Ash), acetone extract percentage (AE), and nitrogen percentage (N), to study genetic diversity. Multivariate [unweighted pair group method with arithmetic means (UPGMA) and Tocher)] and machine learning techniques [K-means and Kohonen's self-organizing maps (SOMs)] were employed. The genotypes showed high genetic variability for some of the evaluated traits. The traits PRI, Ash, and P O contributed the most to genetic diversity. The genotypes were classified into six clusters by the UPGMA method, and the results were consistent with the Tocher, K-means and SOM results. PRI can be used to improve the industrial potential of clones. The clones IAC 418 and PB 326 were the most divergent, followed by IAC 404 and IAC 56. These genotypes and others from the IAC 500 and 400 series could be used to start a breeding program. These combinations offer greater heterotic potential than the others, which can be used to improve components of rubber latex quality. Thus, it is important to consider the quality of rubber latex in the early stage of breeding programs.

Arthroderma benhamiae is a zoophilic dermathophyte that can cause highly inflammatory tinea corporis and tinea capitis in humans. This is the first report of a patient with dermatophytosis caused by A. benhamiae in Brazil. The lesion was an erythematous, annular plaque on the lumbar region that appeared few weeks after playing with a street cat in a 19-month-old girl. Initial presumed diagnosis was tinea corporis caused by Microsporum canis. Topical treatments were ineffective and the patient required systemic treatment with griseofulvin. Mycological diagnosis was inconclusive: morphological differentiation between M. canis and Trichophyton benhamiae may be difficult, especially when the latter present yellow colonies. The etiological agent was identified only by ITS sequencing of the isolates aligned with reference strains to A. benhamiae. This report highlights the importance of ITS sequencing in the identification of isolates from some cases of dermatophytosis, because conventional morphological diagnosis may result in misdiagnosis of the agent and delay proper treatment.

Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for C. neoformans and C. gattii genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different C. neoformans (VNI, VNII, VNIII, and VNIV) and C. gattii (VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMérieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all C. neoformans and C. gattii genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification., (Copyright © 2019 American Society for Microbiology.)

Five different facets or domains of impulsivity (lack of Perseverance, lack of Premeditation, Sensation Seeking, Positive and Negative Urgency) have been detected in undergraduate students by means of a short, 20-item version of the Impulsive Behavior Scale UPPS-P. The present cross-sectional study examined the psychometric properties of a Brazilian version of this short scale (SUPPS-P) in a non-clinical sample of 510 individuals with a larger age range (10-72 years) and from varying socioeconomic strata (SES). We also investigated: (a) differential item functioning according to age, sex and socioeconomic status; (b) whether these demographic factors affected participants' responses (population heterogeneity); and (c) if using scores directly derived from respondents' answers (raw scores) reflected the 5 distinguishable impulsiveness domains out of the structural equation modeling environment (bifactor model). We showed that the short UPPS-P version replicated factor structures, internal consistency across domains and inter-scale correlations found in prior studies, and confirmed the psychometric separability of the 5 impulsiveness domains. Only three out of the 20 items showed differential item functioning. Higher Positive and Negative Urgency and lack of Premeditation were reported by men and impulsiveness decreases with age in all domains except lack of Premeditation. SES did not influence results. The viability of using raw scores to assess the five domains was not confirmed via bifactor modeling. The use of a general composite score was psychometrically acceptable. We conclude that, in the structural equation modeling environment, the SUPPS-P is a reliable instrument to assess multiple impulsivity domains in non-clinical community samples in different cultural settings. However, out of this statistical environment, viability was only found for a general factor of impulsivity.

Background: Current methods for the production of Histoplasma capsulatum antigens are problematic in terms of standardization, specificity, stability, repeatability and reproducibility., Aims: In this study, we sought to optimize the methodology for producing H. capsulatum antigens, and to evaluate its applicability., Methods: Antigenic preparations obtained from 12 H. capsulatum isolates were evaluated by double immunodiffusion and immunoblotting assays against homologous and heterologous sera., Results: The evaluated and optimized protocol allowed a more stable production, as well as repeatable, reproducible, with shorter culture time and less costly. By double immunodiffusion and immunoblotting assays, the best pattern of reactivity was observed for antigens obtained with 33 days of culture from the isolates 200 and 406 against the M antigen and for the isolate 200 with 15 days against H antigen. The SDS-PAGE presented antigenic components of molecular masses between 17 and 119kDa. The immunoblotting sensitivity was 95.5% and 100% with histoplasmosis sera from ill patients and sera from H. capsulatum infected but otherwise healthy patients, respectively, to the antigen derived from isolates 200 and 406., Conclusions: We suggest the employment of the antigen from isolate 200, with 15 or 30 days of culture, in the double immunodiffusion and immunoblotting assays due to its good ability to discriminate both sera from patients with histoplasmosis illness and histoplasmosis infection, in addition to its high specificity against heterologous sera., (Copyright © 2017 Asociación Española de Micología. Publicado por Elsevier España, S.L.U. All rights reserved.)

Several reports showed outbreaks of histoplasmosis acquired while bat-inhabited caves were visited by tourists, miners or researchers. We evaluated the performance of double immunodifusion (DI) and immunoblotting (IB) assays, employed for the histoplasmosis outbreak elucidation occurred in Vale do Paraíba, São Paulo. The existence of epidemiologic link, four patients with clinical signs suggestive of histoplasmosis and mycological confirmation has made that all 35 individuals involved to the cave visit were subjected to serological evaluation. By DI, we observed reactivity against H. capsulatum antigen in a single serum examined nearly 20 days after exposure to fungal propagules. On the other hand, IB showed reactivity against H and M fractions in 50% of samples evaluated. The analysis of the second sample batch, collected two months after the exposure showed that 96.7% were reactive by DI with antibodies titers ranging from 1 to 16 and 100% of reactivity against H and M fractions, by IB, suggesting an acute infection. The analysis of the overall agreement between the methods showed to be reasonable (κ = 0.37). This study confirms the importance and efficacy of more sensitive methodologies, such as IB assay, to early elucidation of disease, especially in cases of patients without mycological information.

Rarely are efforts made to arrive at major theoretical syntheses in the realm of biology, while they are so often successful within the realm of sociology. Furthermore, though these efforts gain paradigmatic status in the former case, in the latter they lead to a 'Balkanization' of the discipline. The article explores what might account for this difference, seeking an answer through the reconstruction of one such effort in biology, which has covered more than two decades.

In his Art and Illusion, art historian Ernst Gombrich argues that the emergence and development of styles of pictorial representation depend on the availability of certain schemata, which can be documented. He himself documents the schemata responsible for the emergence and development of several styles. Nothing of this kind has been done as far as the growth of scientific knowledge is concerned. In this article we discuss whether is it possible to document something analogous to Gombrich's schemata that can be of crucial relevance for the development of clinical research.

We report a case of subcutaneous phaeohyphomycosis observed in a male patient presenting pulmonary sarcoidosis and submitted to corticosteroid treatment. He presented nodular erythematous-violaceous skin lesions in the dorsum of the right hand. Histopathological examination of the biopsied lesion revealed dematiaceous hyphae and yeast-like cells, with a granulomatous tissual reaction. The isolated fungus was identified as Phoma cava. A review of the literature on fungal infection caused by different Phoma species, is presented. The patient healed after therapy with amphotericin B. followed by itraconazole.