1. Extinct type of human parvovirus B19 persists in tonsillar B cells
- Author
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Elina Mäntylä, Lari Pyöriä, Taru Ilmarinen, Klaus Hedman, Maria Söderlund-Venermo, Leena-Maija Aaltonen, Maija Vihinen-Ranta, Mari Toppinen, Lea Hedman, Maria F. Perdomo, Medicum, Department of Virology, Clinicum, Korva-, nenä- ja kurkkutautien klinikka, Human Parvoviruses: Epidemiology, Molecular Biology and Clinical Impact, HUS Head and Neck Center, and Virus infections and immunity
- Subjects
0301 basic medicine ,SYNOVIAL TISSUE ,viruses ,Palatine Tonsil ,General Physics and Astronomy ,Antibodies, Viral ,Genotype ,INFECTION ,Parvovirus B19, Human ,REAL-TIME PCR ,Child ,Cells, Cultured ,B-Lymphocytes ,Multidisciplinary ,biology ,cell type harbouring ,virus diseases ,U937 Cells ,Middle Aged ,3. Good health ,HUMAN ERYTHROVIRUSES ,solut ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,PLASMA-CELLS ,Child, Preschool ,GENETIC DIVERSITY ,Antibody ,Adult ,Cell type ,Adolescent ,BONE-MARROW ,Science ,030106 microbiology ,QUANTITATIVE PCR ,ta3111 ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,Parvoviridae Infections ,Young Adult ,03 medical and health sciences ,Immune system ,Cell Line, Tumor ,medicine ,Humans ,Aged ,B cells ,parvovirus B19 ,Parvovirus ,Monocyte ,ta1182 ,General Chemistry ,DNA ,virus types ,biology.organism_classification ,Virology ,CELLULAR CORECEPTOR ,030104 developmental biology ,Cell culture ,DNA, Viral ,Immunology ,biology.protein ,cells ,3111 Biomedicine - Abstract
Parvovirus B19 (B19V) DNA persists lifelong in human tissues, but the cell type harbouring it remains unclear. We here explore B19V DNA distribution in B, T and monocyte cell lineages of recently excised tonsillar tissues from 77 individuals with an age range of 2–69 years. We show that B19V DNA is most frequent and abundant among B cells, and within them we find a B19V genotype that vanished from circulation >40 years ago. Since re-infection or re-activation are unlikely with this virus type, this finding supports the maintenance of pathogen-specific humoral immune responses as a consequence of B-cell long-term survival rather than continuous replenishment of the memory pool. Moreover, we demonstrate the mechanism of B19V internalization to be antibody dependent in two B-cell lines as well as in ex vivo isolated tonsillar B cells. This study provides direct evidence for a cell type accountable for B19V DNA tissue persistence., The cell type that hosts parvovirus B19 (B19V) DNA lifelong is currently unknown. Here, the authors identify tonsillar B cells as a reservoir, detect an extinct B19V type in older adults, supporting a long-term association, and show that B19V uptake into B cells is antibody dependent.
- Published
- 2017