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To increase use range of distillers’ hops, and to retain the original nutrients and characteristics of distillers’ hops, inlet temperature and outlet temperature and sample concentration has been studied of yield and rehydration rate with orthogonal design, quadratic polynomial stepwise regression, and response surfaces. The results showed that optimum conditions of spray drying is when inlet temperature is 162°C, outlet temperature is 84°C, sample concentration is 3.4g/200ml, feed temperature is 70 ∼ 80°C, fan power is 95%, fluid flow rate is 0.25L/h. In this condition, product yield is 56.432%, rehydration rate is 183.647%.