Search

Additional file 1. Table S1 Strains and plasmids used in this study; Table S2 PCR primers used in this study. Table S3 Data of central composite design; Table S4 The statistical regression results of pretilachlor degradation kinetics; Table S5. Effect of metal ions and EDTA on EthABDB2 enzyme activity; Table S6 Deduced function of each ORF of scaffold 51 sequence containing the missing fragment; Fig. S1 Phylogenetic tree constructed by the neighbour-joining method based on the 16S rRNA gene sequences; Fig. S2 HPLC analysis of pretilachlor degradation by strain TB2 and TB2(pQEth1); Fig. S3 SDS-PAGE(12%) analysis of the purified His6-tagged EthA, EthB, EthC and EthD; Fig. S4 GC/MS analysis of the transformation of alachlor by the EthABDB2; Fig. S5. GC/MS analysis of the transformation of acetochlor by the EthABDB2; Fig. S6 GC/MS analysis of the transformation of propisochlor by the EthABDB2; Fig. S7 GC/MS analysis of the transformation of butachlor by EthABDB2; Fig. S8 effects of pH (A) and temperature (B) on EthABDB2 activity; Fig. S9 HPLC-MS/MS analysis of the pretilachlor and the intermediate metabolites catalyzed by the strain B2.

Additional file of A bifunctional enzyme belonging to cytochrome P450 family involved in the O-dealkylation and N-dealkoxymethylation toward chloroacetanilide herbicides in Rhodococcus sp. B2

Well diffusion method was used to evaluate the antibacterial activity of Eucalyptus camaldulensis, while the antifungal effect was assessed by calculating the reduction percent in the radial growth of mycelia. The inhibition zones exerted by E. camaldulensis crude extracts varied significantly (p ≤ 0.01). The capsule crude extract (acetone 30%) highly inhibited the growth of Acinetobacter baumannii (35 mm clear zone). The highest antifungal activity was against Rhizopus stolonifer with a reduction percent in its radial growth reached to 96%. The bacterial MICs ranged from 20 to 0.5 mg/mL against Escherichia coli and Bacillus subtilis respectively. The MIC values for fungi were between 18 mg/mL (Mucor sp.) and 4 mg/mL (R. stolonifer). Both type and concentration of the solvent greatly affected the antimicrobial potentials of E. camaldulensis. The empty capsule and bud of E. camaldulensis are recognized for the first time as potentially natural resources of effective antimicrobial agents.