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3. Endothelial cell-derived SSAO can increase MLC20 phosphorylation in VSMCs

Author

Zhang Yuxing, Zhang Xiliang, Cao Zhen, Huang Yun, Zheng Yuexin, and Yang Xiaodong

Subjects
ssao, mlc20, inos, vascular hyporesponsiveness, Biology (General), QH301-705.5
Abstract

Vascular hyporesponsiveness in the shock decompensation period is an important factor leading to death. Myosin light chain 20 (MLC20) is the main effector protein that regulates vascular reactivity. However, whether the change in semicarbazide-sensitive amine oxidase (SSAO) expression during hypoxia can change the MLC20 phosphorylation level, and its underlying mechanism were not clear. The amine oxidase copper containing 3 (AOC3) overexpressing adenovirus vector was constructed and transfected into rat intestinal microvascular endothelial cells (RIMECs) to overexpress SSAO, and the RIMECs were co-cultured with rat intestinal microvascular smooth muscle cells (RIMSCs). The changes in SSAO/inducible nitric oxide synthase (iNOS)/Rho associate coiled-coil containing protein kinase 1 (ROCK1) expression levels and MLC20 phosphorylation level were detected. Here we found that the increased SSAO by AOC3 overexpression can decrease the iNOS expression level and its activity after hypoxia. In addition, RIMSCs co-cultured with RIMECs overexpressed with AOC3 gene had significantly higher ROCK1 protein level and MLC20 phosphorylation level than RIMSCs co-cultured with normal RIMECs. Our study demonstrated that SSAO overexpression can significantly inhibit iNOS activity, promote RhoA/ROCK pathway activation, and increase the phosphorylation level of MLC20, which might be the potential mechanism in relieving the vascular hyporesponsiveness during shock decompensation.

Published
2021
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12. Additional file 1 of Functions and clinical significance of KLRG1 in the development of lung adenocarcinoma and immunotherapy

Author

Yang, Xiaodong, Zheng, Yuexin, Han, Zhihai, and Zhang, Xiliang

Abstract

Additional file 1: Supplementary Figure 1. Uncropped western blots used in Fig. 3b. The figure shows all original uncropped blots. The western blots of KLRG1-shRNA3-5 were not shown in the manuscript and Fig. 3b, because they did not shown significant knockdown efficacy. Blots were cropped where indicated by the red lines. Supplementary Figure 2. Uncropped western blots used in Fig. 3e. The figure shows all original uncropped blots. Blots were cropped where indicated by the red lines.

Published
2021
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