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Land use and cover change (LUCC) has a non-negligible impact on both carbon emissions and carbon sinks. Based on the analysis of land use dynamics in Shandong Province, this study simulates land use changes in Shandong Province in 2030 under the Natural Development Scenario (NDS) and Sustainable Development Scenario (SDS), classifies the risk level of carbon emissions in Shandong Province using the Land Use Carbon Emission (LUCE) risk indexes, and compares the differences between the risk level regions under NDS and SDS. This study shows that under the influence of LUCC, the carbon emissions in Shandong province increased significantly, from 90.5591 million tons in 2000 to 493.538 million tons in 2020, with urban land being the main source of carbon emissions, which increased from 90.0757 million tons in 2000 to 490.139 million tons in 2020. The main source of the increase in urban land was cropland. The LUCE was positively correlated with urban land area. The LUCE of SDS was 7.2423 million tons less than that of NDS. From 2000 to 2020, the risk areas of LUCE in Shandong province were mainly no-risk and mild-risk areas. The number of moderate-risk areas and high-risk areas of SDS was less than that of NDS. The rational organization of land use is important for Shandong Province to achieve low-carbon development.

Meng, Zhijun, Huan Gao, Tao Li, Peng Ge, Yixiao Xu, and Binghong Gao. Effects of eight weeks altitude training on the aerobic capacity and microcirculation function in trained rowers. High Alt Med ...

Bacteria play an important role in preventing algal blooms and reducing their harm to the environment. To improve the algicidal activity of Pseudoalteromonas SP48 which had an inhibition effect on dinoflagellate Alexandrium tamarense, its growth medium and fermentation conditions were optimized for this bacterium. In this study, we used two steps to establish the optimum conditions. First, the proper proportion of medium was selected based on an orthogonal design. Then, the fermentation conditions were further optimized through uniform design in an enlarged 5L bioreactor. To test the algicidal ability of Pseudoalteromonas SP48 under the optimum conditions, algal cell morphology was observed by transmission electron microscopy (TEM). After the orthogonal design, we found that the optimum medium was [0.7% (m/v) tryptone, 0.2% (m/v) soluble starch, 0.2% (m/v) sucrose, 0.1% (m/v) FeSO 4, and 1.2% (m/v) K2 HPO 4] for Pseudoalteromonas SP48 growth. Based on these results, optimum fermentation conditions were further explored in a 5L fermentation cylinder using a uniform design; the influence of variables such as incubation time, carbon type, and rotation speed were tested. The optimal fermentation conditions were fermentation time (42 hr), tryptone (1.1%), seeding volume (1.4 × 1013 cells), and rotation speed (250 r/min). Under these established optimum conditions, the biomass of strain SP48 increased by 79.2% and its lethal dose 50% (LD 50) decreased by 54.0%, respectively. The TEM results showed that compared with the control group, the cell wall and cell membrane of A. tamarense were significantly damaged, and the structure and shape of the organelles were destroyed by algicidal bacteria of Pseudoalteromonas SP48. Overall, our results demonstrate that the optimized culture conditions could significantly enhance the algicidal activity of Pseudoalteromonas SP48 against a harmful dinoflagellate, such as A. tamarense. It will effectively provide a scientific foundation for both production of algicidal substances and HABs control.

To investigate the relationship between the left ventricular function and the expression of P-selectin in the serum and cardiac muscle in hemorrhagic shock resuscitation, and to evaluate the effects of L-arginine (L-Arg) against the harmful effect of P-selectin.Thirty SD rats were randomly divided into 3 equal groups: hemorrhagic shock resuscitation (HS) group (undergoing bloodletting until the mean arterial pressure of 40 mm Hg and then re-infusion of the lost blood), L-Arg treatment group (undergoing bloodletting and then re-infusion with L-Arg simultaneously), and normal control (NC) group (undergoing infusion of normal saline). Cannulation was conducted via left carotid artery into the left ventricular to record left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), and left ventricular +/- dp/dtmax. Serum creatine kinase (CK) was detected by colorimetry. Three hours after the HS the rats were killed with samples of blood and apex of heart collected to detect the P-selectin expression by ELISA and immunohistochemistry. Microscopy was used to observe the expression of P-selectin in the vascular endothelial cells and cardiac muscle cells.The LVSP, maximal rate of LV pressure elevation (+ dp/dtmax), and maximal rate of LV pressure depression (- dp/dtmax) of the HS and L-Arg groups were all significantly lower than those of the NC group (all P0.01). The LVEDP of the HS and L-Arg groups were all higher than that of the NC group (both P0.01). Three hours after resuscitation, the CK levels of the HS and L-Arg groups were significantly higher than that of the NC group (both P0.01), and that of the L-Arg groups was significantly lower than that of the HS group (P0.05), the P-selectin levels of the serum and cardiac muscle cells of the HS and L-Arg groups were all significantly higher than those of the NC group (both P0.01), and those of the L-Arg group were significantly lower than those of the HS group (both P0.05).After hemorrhagic shock and resuscitation P-selectin may play an important role in cardiac injury, L-Arg can inhibit the expression of P-selectin, thus protecting the cardiac function against the harmful effect of P-selectin.