Search

Objective To explore the effect of KCNQ1OT1 gene knockout combined with bruceine D on the proliferation, migration, and invasion of breast cancer MDA-MB-231 cells. Methods Cell Counting Kit-8, wound healing, and Transwell invasion assay were used to detect the effects of bruceine D and siKCNQ1OT1 on the viability, migration, and invasion of MDA-MB-231 cells. Effect of bruceine D and siKCNQ1OT1 on the expression of KCNQ1OT1 in MDA-MB-231 cells was detected by qRT-PCR. Western blot was used to detect the effect of bruceine D and siKCNQ1OT1 on the expression of EMT-related proteins and CDC42, p-MKK7, MKK7 proteins in MDA-MB-231 cells. Results Bruceine D and siKCNQ1OT1 could significantly inhibit the viability, migration, and invasion of MDA-MB-231 cells, and the inhibitory effect was enhanced when they were combined (all P < 0.05); bruceine D downregulated the expression of KCNQ1OT1 in MDA-MB-231 cells (all P < 0.05); bruceine D combined with siKCNQ1OT1 significantly decreased CDC42, p-MKK7, N-cadherin, and Vimentin expression in MDA-MB-231 cells and increased the expression of E-cadherin (all P < 0.05). Conclusion Bruceine D combined with siKCNQ1OT1 significantly inhibit the proliferation, migration, invasion, and EMT of human breast cancer MDA-MB-231 cells, and its molecular mechanism may be related to the blocking of CDC42/MKK7 signaling pathway.

A large number of jackup offshore platforms with towers are widely applied in ocean engineering. The dynamic response of the platforms to waves of large wave height is critical, as such waves may cause platform accidents, property damage, and casualties. Therefore, it is important to investigate the coupling effect of jackup offshore platform, towers and seabed foundations under waves of large wave height. In this study, the coupling effect of offshore platforms, tower structures, and seabed foundations under the impact of waves of large wave height was studied via a physical flume model test. The experimental results show that the impact of waves of large wave height on the platforms is significant when the wave is blocked by the platform surface as the water body gathers under the platform surface, causing a pile group effect that results in the onshore piles being subjected to larger pressures than the front ones. The combined action of wave impact and pile leg squeezing force leads to an increase in the pore pressure of the foundation bed near the pile leg, and the soil near the pile leg becomes soft, revealing the mechanism of instability of the offshore platform's pile foundation under waves of large wave height. The acceleration of the longitudinal movement of the platform increases under waves of large wave height, and the vortex-induced vibration of the platform includes the vibration along the direction of the wave and perpendicular to it. A coupled vibration effect between the tower structure and the platform occurs under waves of large wave height, reducing the vibration of the platform itself. Furthermore, damping members are installed on the tower structure, greatly reducing the natural vibration period and the motion response of the tower structure. This study provides significant enlightenment for the design of offshore platforms with towers to protect against waves of large wave height. [ABSTRACT FROM AUTHOR]

This paper reviews the manifestations and achievements of Modi government's "Issue-by-Issue" diplomacy with China since 2016. It discusses the causes and development of India's attitude towards China and analyses India's thinking behind its diplomacy with China. In this paper, an attempt has been made to find out the thinking's influence on India's attitude towards its participation in the Belt and Road Initiatives (BRI). Based on these discussions and analyses, the study concludes that India is suspicious of the BRI. Moreover, whether China can dispel India's suspicion actually does not depend on China's policy orientation towards India and its effects, rather it depends on the specific dividends that the BRI can achieve in other countries in South Asia. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study was to assess Ezrin expression in the primary hepatic carcinoma patients and associated with clinical, pathological characteristics.Materials and Methods: Fifty-one patients with primary hepatocellular carcinoma (PHC) with completed clinical data were retrospectively analyzed in this study. The Ezrin expression in PHC and normal control liver tissue was tested by immunohistochemical assay. The Ezrin expression and relationship with clinical characteristics were evaluated.Results: The Ezrin positive rate were 66.7% and 15.7% with expression score of 3.21 ± 1.46 and 0.60 ± 1.10, respectively, in the cancer tissue and control tissue with statistical difference (P < 0.05). The Ezrin expression was associated with the metastasis status of the patients (P < 0.05) but not associated with the age (P > 0.05), gender (P > 0.05), differentiation (P > 0.05), and tumor diameter (P > 0.05).Conclusion: Ezrin protein is highly expressed in human PHC tissue which can be used for the prediction of metastasis disease. [ABSTRACT FROM AUTHOR]