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172 results on '"Ye, Caiyong"'

1. BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.

Author

Kang, Zhihua, Fu, Pan, Alcivar, Allen L, Fu, Haiqing, Redon, Christophe, Foo, Tzeh Keong, Zuo, Yamei, Ye, Caiyong, Baxley, Ryan, Madireddy, Advaitha, Buisson, Remi, Bielinsky, Anja-Katrin, Zou, Lee, Shen, Zhiyuan, Aladjem, Mirit I, and Xia, Bing

Subjects
BRCA2 Protein, Cell Line, Tumor, Cell Survival, DNA Damage, DNA Helicases, DNA Primase, DNA Replication, DNA, Neoplasm, DNA, Single-Stranded, DNA-Binding Proteins, DNA-Directed DNA Polymerase, Gene Expression Regulation, Neoplastic, Genomic Instability, HEK293 Cells, HeLa Cells, Humans, Minichromosome Maintenance Proteins, Multifunctional Enzymes, Osteoblasts, RNA, Small Interfering, Recombinational DNA Repair, Signal Transduction, Transcription Factors, Breast Cancer, Genetics, Cancer, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning
Abstract

The BRCA2 tumor suppressor protects genome integrity by promoting homologous recombination-based repair of DNA breaks, stability of stalled DNA replication forks and DNA damage-induced cell cycle checkpoints. BRCA2 deficient cells display the radio-resistant DNA synthesis (RDS) phenotype, however the mechanism has remained elusive. Here we show that cells without BRCA2 are unable to sufficiently restrain DNA replication fork progression after DNA damage, and the underrestrained fork progression is due primarily to Primase-Polymerase (PRIMPOL)-mediated repriming of DNA synthesis downstream of lesions, leaving behind single-stranded DNA gaps. Moreover, we find that BRCA2 associates with the essential DNA replication factor MCM10 and this association suppresses PRIMPOL-mediated repriming and ssDNA gap formation, while having no impact on the stability of stalled replication forks. Our findings establish an important function for BRCA2, provide insights into replication fork control during the DNA damage response, and may have implications in tumor suppression and therapy response.

Published
2021

4. Oscillation in microRNA Feedback Loop

Author

Ao, Bin, Zhang, Sheng, Ye, Caiyong, Chang, Lei, Zhou, Guangming, and Yang, Lei

Subjects
Quantitative Biology - Molecular Networks, Nonlinear Sciences - Pattern Formation and Solitons
Abstract

The dynamic behaviors of microRNA and mRNA under external stress are studied with biological experiments and mathematics models. In this study, we developed a mathematic model to describe the biological phenomenon and for the first time reported that, as responses to external stress, the expression levels of microRNA and mRNA sustained oscillation. And the period of the oscillation is much shorter than several reported transcriptional regulation negative feedback loop., Comment: There were some mistakes in the analysis in this paper's first version submitted on 22 Apr 2013. We corrected them in the second version submitted on 23 Sep 2013. So please delete the "v1" version of this paper, Thank you!

Published
2013

5. Warburg Effect due to Exposure to Different Types of Radiation

Author

Bing, Zhitong, Ao, Bin, Zhang, Yanan, Wang, Fengling, Ye, Caiyong, He, Jinpeng, Sun, Jintu, Xiong, Jie, Ding, Nan, Gao, Xiao-fei, Qi, Ji, Zhang, Sheng, Zhou, Guangming, and Yang, Lei

Subjects
Quantitative Biology - Tissues and Organs, Quantitative Biology - Molecular Networks
Abstract

Cancer cells maintain a high level of aerobic glycolysis (the Warburg effect), which is associated with their rapid proliferation. Many studies have reported that the suppression of glycolysis and activation of oxidative phosphorylation can repress the growth of cancer cells through regulation of key regulators. Whether Warburg effect of cancer cells could be switched by some other environmental stimulus? Herein, we report an interesting phenomenon in which cells alternated between glycolysis and mitochondrial respiration depending on the type of radiation they were exposed to. We observed enhanced glycolysis and mitochondrial respiration in HeLa cells exposed to 2-Gy X-ray and 2-Gy carbon ion radiation, respectively. This discovery may provide novel insights for tumor therapy.

Published
2013

6. Vibration Characteristics and Vibration Mitigation Analysis of End Cap PM Excitation Homopolar Inductor Machine

Author

Ye, Caiyong, Qi, Xiaodong, Liu, Kaifeng, Deng, Cong, Fang, Haiyang, and Zhou, Jianlin

Abstract

Vibration analysis and vibration mitigation are very important to ensure the safe and stable operation of electrical machines applied in flywheel energy storage systems. In traditional integer slot synchronous machines, it is generally believed that there is only the zeroth-mode vibration caused by odd tooth harmonics. However, the homopolar inductor machine (HIM) adopts the end cap excitation structure, which makes its air-gap magnetic field contain the dc component, odd tooth harmonics and even tooth harmonics. The force density distribution on the slotted stator is analyzed by the magnetomotive force-multiplying permeance method. The results show that there are both zeroth- and pth(pole pairs)-mode vibrations caused by tooth harmonics and dc component of the air-gap flux density. Furthermore, to reduce vibration and resonance, and to improve the electromagnetic performance of HIM, it is best to use the stator skew slot, the magnetic slot wedge and the sinusoidal rotor slot at the same time. Finally, a 54-slot/6-pole end cap permanent magnet excitation homopolar inductor machine (EPE-HIM) is tested to validate the analytical and the finite-element method (FEM) results, and the variation trend of the experimental results and FEM results is the same.

Published
2023
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19. The windings inductance calculation of an air-core compulsator

Author

Ye, Caiyong, Yu, Kexun, Zhang, Guoping, and Pan, Yuan

Subjects
Electromagnetic fields -- Analysis, Finite element method -- Analysis, Business, Electronics, Electronics and electrical industries
Abstract

An air-core compensated pulse alternator is a compact pulsed power supply for electromagnetic launchers. The structures of these machines are designed distinct from that of traditional synchronous generators. The materials used to build rotor and stator are non-ferromagnetic instead of the traditional ferromagnetic materials. They are usually designed slotless to enhance the stiffness of coupling of windings. As a result, the method of windings inductance calculation is different from that of the traditional ones. The parameter of windings inductance of air-core compensated pulsed alternator plays an important role in the machine design and performance simulation. Therefore, an analytical method to calculate the windings inductance is needed. In this paper, two analytical methods, a 2D method and a 3D method, are used to calculate the windings inductance. As to the special figuration of these windings, a 2D analysis method based on the parallel two-conductor line electromagnetic theory and the superposition principle is employed to calculate the self-inductance and the mutual-inductance of them. Then, a 3D analytical method based on the theory of mutual-inductance between two parallel rectangular loops whose central axes are the same is presented. Finally, a finite-element method based on the software ANSYS is also used to calculate the inductance, which is used to verify the accuracy of the analytical methods. Index Terms--Air-core machine, compulsator, inductance calculation, pulsed alternator, winding design.

Published
2009

37. Requirement of Bccipfor the Regeneration of Intestinal Progenitors

Author

Lu, Huimei, Ye, Caiyong, Liu, Jingmei, Rabson, Arnold B., Verzi, Michael, De, Subhajyoti, and Shen, Zhiyuan

Abstract

BCCIP was originally identified as a BRCA2 and CDKN1A/p21 interaction protein. Although a partial loss of BCCIPfunction is sufficient to trigger genomic instability and tumorigenesis, complete deletion of BCCIPis lethal to cells. Using Rosa26-CreERT2 mouse models, we found that induced Bccipdeletion in adult mice caused an acute intestinal epithelial denudation that cannot be relieved by co-deletion of Trp53. The critical role of Bccipin intestine epithelial renewal was verified with a Villin-CreERT2 mouse model. The epithelium degeneration was associated with a rapid loss of the proliferative capability of the crypt progenitor cells in vivo, lack of crypt base columnar stem cell markers, and a failure of in vitrocrypt organoid growth. RNA-Seq analysis of freshly isolated intestinal crypt cells showed that Bccipdeletion caused an overwhelming down-regulation of genes involved in mitotic cell division but an up-regulation of genes involved in apoptosis and stress response to microbiomes. Our data not only indicate that intestinal epithelium is the most sensitive tissue to whole-body deletion of Bccipbut also point to Bccipas a novel and critical factor for the proliferation of the intestinal progenitors. These findings have significant implications for understanding why a hypomorphic loss of BCCIP functions is more relevant to tumorigenesis.

Published
2021
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38. Cell cycle suspension: A novel process lurking in G2 arrest

Author

He, Jinpeng, Li, Junhong, Ye, Caiyong, Zhou, Libin, Zhu, Jiayun, Wang, Jiayun, Mizota, Atsushi, Furusawa, Yoshiya, and Zhou, Guangming

Abstract

Cell cycle checkpoint is a self-protective mechanism for cells to monitor genome integrity and ensure the high-fidelity transmission of genetic information to daughter cells. Insufficient function of cell cycle checkpoints has been demonstrated to partially account for tumor initiation, promotion and progression. In the ten melanoma cell lines that we tested in preliminary experiments, two human uveal melanoma cell lines, 92-1 and OCM-1, were found to be significantly different in terms of radiosensitivity but similar in DNA repair ability. Evident G2 arrest was induced in both cell types and the maximum was reached at 16 h after irradiation regardless of X-rays or high-LET carbon beams. OCM-1 cells overrode the G2 arrest and reentered the cell cycle right after reaching the maximum, whereas 92-1 could not. Upon 10 Gy of radiation, the cell cycle of 92-1 was suspended and remained unchanged for up to 5 d. The cell cycle suspension is a unique process lurking in G2 arrest and related to cellular radiosensitivity. Its induction is dose-dependent and there is a dose threshold for it. The degradation of Cyclin B1 has been found related to the cell cycle suspension though, the mechanism of cell cycle suspension is still under investigation. Basing on our knowledge, this is the first report on cell cycle suspension and we present here a de novo mechanism to cellular radiosensitivity. Further clarification of the mechanism underlying cell cycle suspension is believed to be of significance in tumor radiosensitization or even direct tumor control.

Published
2011

45. A Novel Hybrid Excited Doubly Salient Machine With Asymmetric Stator Poles.

Author

He, Mingjie, Xu, Wei, Zhu, Jianguo, Ning, Lei, Du, Guanghui, and Ye, Caiyong

Subjects
PERMANENT magnet generators, AIR gap flux, MAGNETIC circuits, MACHINING, ACTINIC flux, STATORS, POLISH people
Abstract

A novel hybrid excited asymmetric stator pole doubly salient machine (HEASPDSM) is proposed in this paper. The new topologies and operational principle are first investigated by using a simplified equivalent magnetic circuit model. The harmonic components of air-gap flux density are then analyzed. The influence of key structural parameters of the proposed machine on the flux regulation ability and torque is studied. A full comparison on main electromagnetic performance indexes of the proposed 12/7 (stator/rotor poles) HEASPDSPM and several traditional doubly salient machines is presented. A prototype is fabricated and tested to validate the theoretical analysis. The results well demonstrate that the HEASPDSPM possesses various merits, such as strong flux regulation capability, high torque density, low cogging torque, and low torque ripple. [ABSTRACT FROM AUTHOR]

Published
2019
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