109 results on '"Yasuharu Watanabe"'
Search Results
2. INVESTIGATION OF RIVER STRUCTURES DAMAGED AT CHIKUMA RIVER DUE TO TYPHOON NO. 19, OCTOBER 2019
- Author
-
Kenji Watanabe, Shunzo Kawajiri, Tomoya Matsuda, and Yasuharu Watanabe
- Subjects
Hydrology ,Environmental Engineering ,Typhoon ,Environmental science ,Civil and Structural Engineering - Published
- 2022
- Full Text
- View/download PDF
3. LARGE-SCALE OPEN-CHANNEL EXPERIMENTS ON THE COLLAPSE OF ABUTMENT BACKFILL OWING TO FLOODS AND PROPOSED COUNTERMEASURE
- Author
-
Shunzo Kawajiri, Kohei Onmayashiki, Masaki Koyama, Yasunori Miyamori, Takayuki Kawaguchi, Yasuharu Watanabe, and Tomoya Matsuda
- Subjects
Environmental Engineering ,Countermeasure ,Flood myth ,Scale (ratio) ,Abutment ,Collapse (topology) ,Geotechnical engineering ,Geology ,Civil and Structural Engineering ,Open-channel flow - Abstract
In Japan, great damage to abutment backfills caused by large-scale floods occurs every year. It is necessary to examine the fundamental collapse mechanism on as close to full scale as possible, in order to achieve a sufficient effect of the similarity laws in geotechnical engineering, river engineering, and bridge engineering. In this study, geotechnical evaluations were carried out, such as observation of pore water pressure behavior in an embankment, settlement of an embankment crest, and applicability of a reinforced soil wall as a countermeasure construction, under conditions affected by flowing water. The results show that the collapse behavior of an embankment surface differs depending on the soil properties of the embankment material. The erosion of the river bed progressed even in the footing lower surface of the abutment foundation and in the upstream side of the abutment, where the flow velocity was locally large and changes in the flow direction were confirmed. In addition, when a gabion-reinforced earth wall with water permeability was used for the wall surface construction as a countermeasure, washout of the embankment in the abutment to the extent that a pavement surface above would collapse did not occur, thereby confirming the gabion’s usefulness as a countermeasure.
- Published
- 2021
- Full Text
- View/download PDF
4. Isoliquiritigenin Attenuates Adipose Tissue Inflammation and Metabolic Syndrome by Modifying Gut Bacteria Composition in Mice
- Author
-
Riko Ishibashi, Yukihiro Furusawa, Hiroe Honda, Yasuharu Watanabe, Shiho Fujisaka, Miyu Nishikawa, Shinichi Ikushiro, Shin Kurihara, Yoshiaki Tabuchi, Kazuyuki Tobe, Kiyoshi Takatsu, and Yoshinori Nagai
- Subjects
Inflammation ,Metabolic Syndrome ,Bacteria ,Anti-Inflammatory Agents ,Diet, High-Fat ,Mice, Inbred C57BL ,Mice ,Chalcones ,Glucose ,Adipose Tissue ,Animals ,Insulin Resistance ,Food Science ,Biotechnology - Abstract
Isoliquiritigenin (ILG) has been reported to attenuate adipose tissue inflammation and metabolic disorder; however, the underlying mechanisms remain to be elucidated. The aim of this study is to elucidate whether ILG shows the anti-inflammatory and antimetabolic syndrome effects through gut microbiota modification.Mice are fed a high-fat diet (HFD) with or without ILG for up to 12 weeks. The effect of ILG on body weight, blood glucose level, adipose tissue inflammation, gut barrier function, and gut microbiota composition are investigated. ILG supplementation alleviates HFD-induced obesity, glucose tolerance, and insulin resistance and suppresses inflammatory gene expression in epididymal white adipose tissue (eWAT). Moreover, ILG supplementation modifies gut bacterial composition by increasing the abundance of antimetabolic disease-associated species (e.g., Parabacteroides goldsteinii and Akkemansia muciniphila) and up-regulated genes associated with gut barrier function. Fecal microbiome transplantation (FMT) from ILG-fed donors counteract HFD-induced body and eWAT weight changes, inflammation-related gene expression, glucose tolerance, and insulin resistance, thereby suggesting that ILG-responsive gut bacteria exerts anti-inflammatory and antimetabolic syndrome effects.Alterations in gut bacteria underly the beneficial effects of ILG against adipose tissue inflammation and metabolic disorders. ILG may be a promising prebiotic for the prevention and treatment of metabolic syndrome.
- Published
- 2022
5. Bidirectional crosstalk between neutrophils and adipocytes promotes adipose tissue inflammation
- Author
-
Makoto Arita, Shun'ichiro Taniguchi, Yasuharu Watanabe, Tsutomu Yanagibashi, Yoshinori Nagai, Hiromitsu Hara, Masaru Ogasawara, Naoki Okamoto, Hiroe Honda, Ichiro Takasaki, Ryu Imamura, Kiyoshi Takatsu, Masakiyo Sasahara, Shigeaki Hida, Takashi Suda, and Seiji Yamamoto
- Subjects
0301 basic medicine ,Inflammasomes ,Neutrophils ,Leukotriene B4 ,Adipose Tissue, White ,Lipolysis ,Adipose tissue ,Mice, Transgenic ,Inflammation ,White adipose tissue ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Adipocytes ,Genetics ,medicine ,Animals ,Obesity ,Molecular Biology ,Macrophages ,Inflammasome ,Chemotaxis ,Cell biology ,Crosstalk (biology) ,030104 developmental biology ,Adipose Tissue ,chemistry ,medicine.symptom ,030217 neurology & neurosurgery ,Biotechnology ,medicine.drug - Abstract
Chronic activation of the IL-1β system in adipose tissue on metabolic disorders is well demonstrated. However, a mechanism for its expression and activation in the tissue has remained unexplored. Here, we demonstrate that IL-1β transcript was enriched in neutrophils of white adipose tissue (WAT) from lean mice. Mechanistically, the interaction of neutrophils with adipocytes induced IL-1β expression via NF-κB pathway. Lipolysis of adipocytes accumulated neutrophils prior to macrophages in WAT and produced high levels of IL-1β via an inflammasome pathway. Leukotriene B4 (LTB4) production in WAT also contributed to neutrophil accumulation. Furthermore, an LTB4-inflammasome axis contributed to the expression of chemotactic molecules involved in high-fat diet-induced macrophage infiltration into WAT. We have identified previously unappreciated roles for neutrophils in the development of adipose tissue inflammation: robust IL-1β production and infiltration of macrophages to initiate chronic inflammation.-Watanabe, Y., Nagai, Y., Honda, H., Okamoto, N., Yanagibashi, T., Ogasawara, M., Yamamoto, S., Imamura, R., Takasaki, I., Hara, H., Sasahara, M., Arita, M., Hida, S., Taniguchi, S., Suda, T., Takatsu, K. Bidirectional crosstalk between neutrophils and adipocytes promotes adipose tissue inflammation.
- Published
- 2019
- Full Text
- View/download PDF
6. Investigation report of geotechnical disaster on river area due to typhoon landfall three times on Okhotsk region, Hokkaido, Japan
- Author
-
Dai Nakamura, Yasuharu Watanabe, Satoshi Yamashita, Shunzo Kawajiri, Takayuki Kawaguchi, Hiroshi Hayakawa, and Yasunori Miyamori
- Subjects
021110 strategic, defence & security studies ,geography ,geography.geographical_feature_category ,Flooding (psychology) ,Torrential rain ,0211 other engineering and technologies ,Abutment ,02 engineering and technology ,Geotechnical Engineering and Engineering Geology ,Typhoon ,Erosion ,Geotechnical engineering ,Soil properties ,Levee ,Geology ,021101 geological & geomatics engineering ,Civil and Structural Engineering ,Landfall - Abstract
For one week from August 17 to 23, 2016, three consecutive typhoons made landfall in Hokkaido for the first time on record. These typhoons and the front they stimulated brought record-breaking torrential rain over the eastern part of Hokkaido. To investigate the damage to grounds and rivers resulting from this rainfall, the Japan Society of Civil Engineers (JSCE) and the Japanese Geotechnical Society (JGS) formed a disaster research group to conduct an investigation. This report provides the results of the investigation into damage to the grounds of areas along the Tokoro River of the Okhotsk region, Hokkaido, that suffered from this tremendous and diverse disaster. Specifically, the report describes the situation of the levees which were broken and eroded by the overflowing water, the shape of the levee bodies, the levee body soil properties examined by observation of the sections, as well as the occurrence of sand boiling and air blows. The washout of road embankments as well as damage to road bridge mounting fills and abutment backfills were also investigated. The investigation has demonstrated the need to clarify the resistance of the abutment backfills and levee bodies to flowing water as well as the geotechnical predominant factors in order to clarify the mechanisms behind erosion and washout, the need to review new measures that allow for the scale of sand boiling and resultant changes in levee body stability, and the fact that the existing embankments were able to temporarily suppress the flooding water which had spilled over from the river. Furthermore, although it has been identified that the findings of a study on an embankment washout associated with a tsunami can be applied to measures taken against the overflowing water, it has also been found necessary to clarify the predominant geotechnical factors using model tests and to use a more sophisticated analytical approach to establish a geotechnical stability review as soon as possible in order to prevent the levees and embankments from being eroded and washed out due to overflowing water.
- Published
- 2019
- Full Text
- View/download PDF
7. Funiculosin variants and phosphorylated derivatives promote innate immune responses via the Toll-like receptor 4/myeloid differentiation factor-2 complex
- Author
-
Toshiyuki Shimizu, Akinori Saeki, Shigeto Fujishita, Takahito Kimura, Hiroe Honda, Tatsuhisa Kato, Kensuke Miyake, Tomomi Yamaguchi-Miyamoto, Natsuko Tanimura, Naoki Okamoto, Ryutaro Fukui, Yoshinori Nagai, Yuji Motoi, B. Umeharu Ohto, Yukari Fujimoto, Koichi Fukase, Kiyoshi Takatsu, Takatsugu Hirokawa, Keisuke Mizote, Yasuharu Watanabe, and Sachiko Akashi-Takamura
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Pyridones ,Recombinant Fusion Proteins ,Immunology ,Lymphocyte Antigen 96 ,Bone Marrow Cells ,Biology ,Ligands ,Biochemistry ,Cell Line ,Structure-Activity Relationship ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Animals ,Humans ,Phosphorylation ,Receptor ,Molecular Biology ,Cells, Cultured ,Mice, Knockout ,Toll-like receptor ,Binding Sites ,Innate immune system ,Macrophages ,Models, Immunological ,Computational Biology ,Dendritic Cells ,Cell Biology ,Dendritic cell ,Molecular biology ,Immunity, Innate ,Specific Pathogen-Free Organisms ,Mice, Inbred C57BL ,Molecular Docking Simulation ,Toll-Like Receptor 4 ,030104 developmental biology ,Drug Design ,TLR4 ,Fetal bovine serum ,030215 immunology - Abstract
The Toll-like receptor 4 (TLR4)/myeloid differentiation factor-2 (MD-2) complex is essential for LPS recognition and induces innate immune responses against Gram-negative bacteria. As activation of TLR4/MD-2 is also critical for the induction of adaptive immune responses, TLR4/MD-2 agonists have been developed as vaccine adjuvants, but their efficacy has not yet been ascertained. Here, we demonstrate that a funiculosin (FNC) variant, FNC-RED, and FNC-RED and FNC derivatives are agonists for both murine and human TLR4/MD-2. FNC-RED induced nuclear factor-κB (NF-κB) activation via murine TLR4/MD-2, whereas FNC had no TLR4/MD-2 stimulatory activity. Biacore analysis revealed that FNC-RED binds to murine TLR4/MD-2 but not murine radioprotective 105 (RP105)/myeloid differentiation factor-1 (MD-1), another LPS sensor. FNC-RED induced CD14-independent expressions of pro-inflammatory cytokines and co-stimulatory molecules in murine macrophages and dendritic cells. In contrast, FNC-RED stimulation was reduced in CD14-dependent LPS responses, including dimerization and internalization of TLR4/MD-2 and IFN-β expression. FNC-RED-induced IL-12p40 production from murine dendritic cells was dependent on NF-κB but not MAPK pathway. In addition, fetal bovine serum augmented lipid A-induced NF-κB activation but blocked FNC-RED-mediated responses. Two synthetic phosphate group-containing FNC-RED and FNC derivatives, FNC-RED-P01 and FNC-P01, respectively, activated human TLR4/MD-2, unlike FNC-RED. Finally, computational analysis revealed that this species-specific activation by FNC-RED and FNC-RED-P01 resulted from differences in electrostatic surface potentials between murine and human TLR4/MD-2. We conclude that FNC-RED and its synthetic derivative represent a novel category of murine and human TLR4/MD-2 agonist.
- Published
- 2017
- Full Text
- View/download PDF
8. HIF-1α in Myeloid Cells Promotes Adipose Tissue Remodeling Toward Insulin Resistance
- Author
-
Kazuyuki Tobe, Kiyoshi Takatsu, Aminuddin Aminuddin, Yasuharu Watanabe, Koizumi Keiichi, Michihiro Matsumoto, Akiko Takikawa, Arshad Mahmood, Masashi Ikutani, Keisuke Okabe, Takashi Nakagawa, Kumiko Saeki, Shiho Fujisaka, Masakiyo Sasahara, Nobuhito Goda, Yoshiko Igarashi, Satoko Senda, Johji Imura, Tomonobu Kado, Isao Usui, Koichi Tsuneyama, Yoshinori Nagai, Allah Nawaz, and Seiji Yamamoto
- Subjects
Male ,Vascular Endothelial Growth Factor A ,0301 basic medicine ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Adipose tissue macrophages ,Adipose tissue ,Inflammation ,White adipose tissue ,Biology ,Diet, High-Fat ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Insulin resistance ,Fibrosis ,3T3-L1 Cells ,Internal medicine ,Angiopoietin-1 ,Internal Medicine ,medicine ,Animals ,Myeloid Cells ,Cells, Cultured ,Mice, Knockout ,Neovascularization, Pathologic ,Macrophages ,Glucose Tolerance Test ,Hypoxia-Inducible Factor 1, alpha Subunit ,medicine.disease ,Mice, Inbred C57BL ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,030104 developmental biology ,Endocrinology ,Adipose Tissue ,chemistry ,Fibroblast Growth Factor 1 ,Female ,Insulin Resistance ,medicine.symptom ,Fibroblast Growth Factor 10 - Abstract
Adipose tissue hypoxia is an important feature of pathological adipose tissue expansion. Hypoxia-inducible factor-1α (HIF-1α) in adipocytes reportedly induces oxidative stress and fibrosis, rather than neoangiogenesis via vascular endothelial growth factor (VEGF)-A. We previously reported that macrophages in crown-like structures (CLSs) are both hypoxic and inflammatory. In the current study, we examined how macrophage HIF-1α is involved in high-fat diet (HFD)–induced inflammation, neovascularization, hypoxia, and insulin resistance using mice with myeloid cell–specific HIF-1α deletion that were fed an HFD. Myeloid cell–specific HIF-1α gene deletion protected against HFD-induced inflammation, CLS formation, poor vasculature development in the adipose tissue, and systemic insulin resistance. Despite a reduced expression of Vegfa in epididymal white adipose tissue (eWAT), the preadipocytes and endothelial cells of HIF-1α–deficient mice expressed higher levels of angiogenic factors, including Vegfa, Angpt1, Fgf1, and Fgf10 in accordance with preferable eWAT remodeling. Our in vitro study revealed that lipopolysaccharide-treated bone marrow–derived macrophages directly inhibited the expression of angiogenic factors in 3T3-L1 preadipocytes. Thus, macrophage HIF-1α is involved not only in the formation of CLSs, further enhancing the inflammatory responses, but also in the inhibition of neoangiogenesis in preadipocytes. We concluded that these two pathways contribute to the obesity-related physiology of pathological adipose tissue expansion, thus causing systemic insulin resistance.
- Published
- 2016
- Full Text
- View/download PDF
9. Restoration of the shifting mosaic of floodplain forests under a flow regime altered by a dam
- Author
-
Satoshi Yamanaka, Yuki Yabuhara, Yasuharu Watanabe, Futoshi Nakamura, Miwa Konno, Junjiro N. Negishi, Takumi Akasaka, and Akira Terui
- Subjects
Return period ,Environmental Engineering ,Floodplain ,Riparian ecosystem ,Environmental flow ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,01 natural sciences ,Regeneration (ecology) ,Salix arbutifolia ,0105 earth and related environmental sciences ,Nature and Landscape Conservation ,Riparian zone ,Hydrology ,geography ,geography.geographical_feature_category ,Flood myth ,Gravel-bed river ,Sediment ,04 agricultural and veterinary sciences ,Habitat ,040103 agronomy & agriculture ,River morphology ,0401 agriculture, forestry, and fisheries ,Environmental science ,Artificial flood - Abstract
A braided gravel-bed river provides essential habitats for various plants and animals. However, human regulation of rivers, such as dams, channelization, and other engineering works, alter flow and sediment regimes, which generally cause the degradation of river and riparian ecosystems. One of the prominent changes prevailing in Japanese rivers is forest expansion over gravel bars, and many native plants and animals that depend on gravel-bar habitat are now endangered. The Satsunai River is typical of rivers experiencing forest expansion, so the Japanese government launched a restoration project in 2012 to partially restore its riparian ecosystems. This is a large-scale experiment developed jointly by an interdisciplinary science team and river managers, who conduct monitoring and evaluation under an adaptive management scheme. The main measure to restore gravel bed habitat was an artificial flood regime, releasing a maximum water volume of 120 m3/s, which was a 2-year return period flood before dam construction. A unique feature of the project is that we considered the role of high-magnitude floods with recurrence intervals greater than 20 years after dam construction. We hypothesize that the artificial floods can be timed seasonally to create sites for regeneration and nesting of riparian species, and the high-magnitude floods contribute to maintaining a shifting mosaic structure of floodplain forest and unvegetated gravel-bar patches. We also used critical non-dimensional shear stress analysis to define “flood-disturbance areas” that can be disturbed under the artificial flow regime created by a dam. Artificial floods have been initiated once a year since 2012 at the end of June, synchronized with the seed dispersal period of Salix arbutifolia, which is endangered and a high conservation priority in the project. Thus far, the idea of setting floodplain-disturbance areas and the strategy of using both artificial and high-magnitude floods to restore a shifting mosaic of floodplain habitat patches is appropriate, as we found successful regeneration of S. arbutifolia and an exponential decay curve of the age distribution of floodplain forest patches. However, the sediment regime regulated by the dam was not addressed in this research, so future monitoring should track changes in river morphology associated with reduced sediment supply caused by the dam.
- Published
- 2020
- Full Text
- View/download PDF
10. Differential requirements of MyD88 and TRIF pathways in TLR4-mediated immune responses in murine B cells
- Author
-
Yasuharu Watanabe, Tsutomu Yanagibashi, Kiyoshi Takatsu, Masashi Ikutani, Yoshikatsu Hirai, and Yoshinori Nagai
- Subjects
LPS ,Immunology ,chemical and pharmacologic phenomena ,Biology ,Mice ,Immune system ,Interferon ,TLR ,medicine ,Activation-induced (cytidine) deaminase ,Animals ,Immunology and Allergy ,CSR ,TRIF ,Interleukin 4 ,Mice, Knockout ,Innate immunity ,CD86 ,B-Lymphocytes ,Innate immune system ,MyD88 ,Molecular biology ,Toll-Like Receptor 4 ,Adaptor Proteins, Vesicular Transport ,Immunoglobulin class switching ,Immunoglobulin G ,Antibody Formation ,Myeloid Differentiation Factor 88 ,biology.protein ,Signal Transduction ,medicine.drug - Abstract
LPS stimulates the TLR4/Myeloid differentiation protein-2 (MD-2) complex and promotes a variety of immune responses in B cells. TLR4 has two main signaling pathways, MyD88 and Toll/IL-1R (TIR)-domain-containing adaptor-inducing interferon-β (TRIF) pathways, but relatively few studies have examined these pathways in B cells. In this study, we investigated MyD88- or TRIF-dependent LPS responses in B cells by utilizing their knockout mice. Compared with wild-type (WT) B cells, MyD88(-/-) B cells were markedly impaired in up-regulation of CD86 and proliferation induced by lipid A moiety of LPS. TRIF(-/-) B cells were also impaired in these responses compared with WT B cells, but showed better responses than MyD88(-/-) B cells. Regarding class switch recombination (CSR) elicited by lipid A plus IL-4, MyD88(-/-) B cells showed similar patterns of CSR to WT B cells. However, TRIF(-/-) B cells showed the impaired in the CSR. Compared with WT and MyD88(-/-) B cells, TRIF(-/-) B cells exhibited reduced cell division, fewer IgG1(+) cells per division, and decreased activation-induced cytidine deaminase (Aicda) mRNA expression in response to lipid A plus IL-4. Finally, IgG1 production to trinitrophenyl (TNP)-LPS immunization was impaired in TRIF(-/-) mice, while MyD88(-/-) mice exhibited increased IgG1 production. Thus, MyD88 and TRIF pathways differently regulate TLR4-induced immune responses in B cells.
- Published
- 2015
- Full Text
- View/download PDF
11. Isoliquiritigenin: A Unique Component That Attenuates Adipose Tissue Inflammation and Fibrosis by Targeting the Innate Immune Sensors
- Author
-
Hiroe Honda, KiyoshiTakatsu, Yoshinori Nagai, and Yasuharu Watanabe
- Subjects
chemistry.chemical_compound ,Innate immune system ,Chemistry ,Fibrosis ,Immunology ,medicine ,Adipose tissue ,Inflammation ,medicine.symptom ,medicine.disease ,Isoliquiritigenin - Published
- 2017
12. Development of a meandering channel caused by the planform shape of the river bank
- Author
-
T. Nagata, A. Ito, H. Yasuda, and Yasuharu Watanabe
- Subjects
Hydrology ,Dike ,geography ,geography.geographical_feature_category ,lcsh:Dynamic and structural geology ,Flood myth ,Flow (psychology) ,Front (oceanography) ,Point bar ,Geophysics ,lcsh:QE500-639.5 ,Typhoon ,Geomorphology ,Bank ,Bank erosion ,Earth-Surface Processes - Abstract
Due to a typhoon and a stationary rain front, record amounts of rain fell in September 2011, and the largest class of discharge in recorded history was observed in the Otofuke River of eastern Hokkaido in Japan, and extensive bank erosion occurred in various parts of the river channel. Damages were especially serious in the middle reaches, where part of a dike was washed out. The results of a post-flood survey suggested that the direct cause of the dike breach was lateral advance of the bank erosion associated with the development of meandering channels. As the related development mechanism and predominant factors have not yet been clarified, this remains a priority from the viewpoint of disaster prevention. A past study on the development of meandering channels was reported by Shimizu et al. (1996). In this study, the meandering channel development process was reproduced using a slope failure model that linked bank erosion with bed changes. The study attempted to clarify the meandering development mechanism in the disaster and its predominant factors by using this model. The analysis properly reproduced the characteristics of the post-flood meandering waveforms. Therefore, it is suggested that the development of meandering during the flood attributed to the propagation of meandering downstream, which is triggered by the meandering flow from the meandering channel in the upstream, which also suggests that this propagated meandering then caused a gradual increase of meandering amplitude accompanied by bank erosion in the recession period of the flood., c Author(s) 2014. This work is distributed under the Creative Commons Attribution 3.0 License.
- Published
- 2014
- Full Text
- View/download PDF
13. The TLR family protein RP105/MD-1 complex
- Author
-
Yasuharu Watanabe, Kiyoshi Takatsu, and Yoshinori Nagai
- Subjects
metabolic disorder ,chronic inflammation ,Toll-like receptor ,medicine.medical_specialty ,Histology ,FGF21 ,business.industry ,Mini Review ,Adipose tissue macrophages ,Adipose tissue ,Inflammation ,Cell Biology ,medicine.disease ,Endocrinology ,Insulin resistance ,insulin resistance ,Internal medicine ,TLR4 ,Medicine ,medicine.symptom ,business ,Receptor ,innate immunity - Abstract
The radioprotective 105 (RP105)/MD-1 complex is a member of the Toll-like receptor (TLR) family of proteins. We have previously reported that this complex cooperates with the essential lipopolysaccharide (LPS) receptor TLR4/MD-2 complex and plays a crucial role in LPS responses by B cells. Recent evidences suggest that TLRs can also recognize endogenous ligands and promote non-infectious chronic inflammation. For instance, TLR4/MD-2 can be ligated by adipose tissue-derived saturated free fatty acids (FAs) and induce adipose tissue inflammation and insulin resistance. Recently, we reported that RP105 knockout (KO) or MD-1 KO mice have less high-fat diet (HFD)-induced obesity, adipose tissue inflammation and insulin resistance than wild-type (WT) or TLR4 KO mice. As RP105/MD-1 is not involved in recognition of palmitic and stearic acids, which are endogenous ligands for TLR4/MD-2, we conclude that RP105/MD-1 is itself a key regulator of diet-induced chronic inflammation in adipose tissue, obesity and insulin resistance that appears to be independent of the TLR4-dependent pathway. In this mini-review, we will highlight the significance of the RP105/MD-1 complex in adipose tissue inflammation and discuss implications for human diseases.
- Published
- 2013
- Full Text
- View/download PDF
14. Isoliquiritigenin Attenuates Adipose Tissue Inflammation in vitro and Adipose Tissue Fibrosis through Inhibition of Innate Immune Responses in Mice
- Author
-
Seiji Yamamoto, Yasuharu Watanabe, Naoki Okamoto, Yoshinori Nagai, Masakiyo Sasahara, Hiroe Honda, Kensuke Miyake, Takayoshi Suganami, Miyako Tanaka, Kiyoshi Takatsu, Yoko Ishii, and Takeru Hamashima
- Subjects
0301 basic medicine ,Lipopolysaccharides ,Male ,medicine.medical_specialty ,Inflammasomes ,Adipose tissue macrophages ,Adipose Tissue, White ,Anti-Inflammatory Agents ,Adipose tissue ,Inflammation ,Biology ,Diet, High-Fat ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Interferon-gamma ,Chalcones ,Fibrosis ,Internal medicine ,Paracrine Communication ,medicine ,Adipocytes ,Animals ,Multidisciplinary ,Tumor Necrosis Factor-alpha ,Macrophages ,NF-kappa B ,Inflammasome ,Cell Differentiation ,Stromal vascular fraction ,medicine.disease ,Coculture Techniques ,Immunity, Innate ,Mice, Inbred C57BL ,030104 developmental biology ,Endocrinology ,chemistry ,TLR4 ,medicine.symptom ,Isoliquiritigenin ,medicine.drug - Abstract
Isoliquiritigenin (ILG) is a flavonoid derived from Glycyrrhiza uralensis and potently suppresses NLRP3 inflammasome activation resulting in the improvement of diet-induced adipose tissue inflammation. However, whether ILG affects other pathways besides the inflammasome in adipose tissue inflammation is unknown. We here show that ILG suppresses adipose tissue inflammation by affecting the paracrine loop containing saturated fatty acids and TNF-α by using a co-culture composed of adipocytes and macrophages. ILG suppressed inflammatory changes induced by the co-culture through inhibition of NF-κB activation. This effect was independent of either inhibition of inflammasome activation or activation of peroxisome proliferator-activated receptor-γ. Moreover, ILG suppressed TNF-α-induced activation of adipocytes, coincident with inhibition of IκBα phosphorylation. Additionally, TNF-α-mediated inhibition of Akt phosphorylation under insulin signaling was alleviated by ILG in adipocytes. ILG suppressed palmitic acid-induced activation of macrophages, with decreasing the level of phosphorylated Jnk expression. Intriguingly, ILG improved high fat diet-induced fibrosis in adipose tissue in vivo. Finally, ILG inhibited TLR4- or Mincle-stimulated expression of fibrosis-related genes in stromal vascular fraction from obese adipose tissue and macrophages in vitro. Thus, ILG can suppress adipose tissue inflammation by both inflammasome-dependent and -independent manners and attenuate adipose tissue fibrosis by targeting innate immune sensors.
- Published
- 2016
- Full Text
- View/download PDF
15. The Radioprotective 105/MD-1 Complex Contributes to Diet-Induced Obesity and Adipose Tissue Inflammation
- Author
-
Kazuyuki Tobe, Koichi Tsuneyama, Masataka Sata, Yoshinori Nagai, Yoshinori Ichihara, Toshiyasu Sasaoka, Shizuo Akira, Hiro-omi Kanayama, Tomoya Nakamura, Yoichiro Hirata, Hirofumi Izaki, Kiyoshi Takatsu, Shiho Fujisaka, Sho Ishikawa, Yasuharu Watanabe, Michio Shimabukuro, Kensuke Miyake, Isao Usui, Yoshihiro Ogawa, Tsutomu Wada, and Takayoshi Suganami
- Subjects
Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Adipose tissue macrophages ,Palmitic Acid ,Adipose tissue ,Inflammation ,White adipose tissue ,Biology ,Mice ,Insulin resistance ,Antigens, CD ,Internal medicine ,Internal Medicine ,medicine ,Adipocytes ,Animals ,Humans ,Obesity ,Epididymis ,Mice, Knockout ,Membrane Glycoproteins ,Macrophages ,Fatty liver ,Stromal vascular fraction ,medicine.disease ,Dietary Fats ,Coculture Techniques ,Fatty Liver ,Mice, Inbred C57BL ,Toll-Like Receptor 4 ,Endocrinology ,Adipose Tissue ,Gene Expression Regulation ,Antigens, Surface ,TLR4 ,medicine.symptom ,Immunology and Transplantation ,Insulin Resistance ,Stearic Acids - Abstract
Recent accumulating evidence suggests that innate immunity is associated with obesity-induced chronic inflammation and metabolic disorders. Here, we show that a Toll-like receptor (TLR) protein, radioprotective 105 (RP105)/myeloid differentiation protein (MD)-1 complex, contributes to high-fat diet (HFD)-induced obesity, adipose tissue inflammation, and insulin resistance. An HFD dramatically increased RP105 mRNA and protein expression in stromal vascular fraction of epididymal white adipose tissue (eWAT) in wild-type (WT) mice. RP105 mRNA expression also was significantly increased in the visceral adipose tissue of obese human subjects relative to nonobese subjects. The RP105/MD-1 complex was expressed by most adipose tissue macrophages (ATMs). An HFD increased RP105/MD-1 expression on the M1 subset of ATMs that accumulate in eWAT. Macrophages also acquired this characteristic in coculture with 3T3-L1 adipocytes. RP105 knockout (KO) and MD-1 KO mice had less HFD-induced adipose tissue inflammation, hepatic steatosis, and insulin resistance compared with wild-type (WT) and TLR4 KO mice. Finally, the saturated fatty acids, palmitic and stearic acids, are endogenous ligands for TLR4, but they did not activate RP105/MD-1. Thus, the RP105/MD-1 complex is a major mediator of adipose tissue inflammation independent of TLR4 signaling and may represent a novel therapeutic target for obesity-associated metabolic disorders.
- Published
- 2012
16. THE PHENOMENON OF BREAKUP IN A FROZEN RIVER AND DEVELOPMENT OF A PRACTICAL FORMULA FOR ICE SHEET THICKNESS CALCULATION
- Author
-
Yasuharu Watanabe, Yasuyuki Hirai, Hiroshi Hayakawa, and Yasuhiro Yoshikawa
- Subjects
geography ,geography.geographical_feature_category ,Ice sheet ,Breakup ,Geomorphology ,Geology ,Field observation - Abstract
結氷河川の解氷現象は,水位の急激な上昇を引き起こすアイスジャムの発生の可能性がある.河川結氷時の河道内工事や流量観測を実施する際には,作業計画および安全面から,解氷現象に関する知見および河道内の氷板厚を計算する技術が求められている.結氷河川における解氷現象を解明するために,氷板厚を連続的に測定し,熱フラックスを用いた氷板厚計算モデルを用いて,放射・対流,積雪,流水の3因子が氷板厚の変動に与える影響を評価した.本計算モデルを基にして,積雪と晶氷の影響を係数α,河氷の融解の影響を係数βに織り込み,気温,水温,有効水深から氷板厚を計算する実用的な計算式を開発した.さらに,気温から水温を計算し,有効水深を一定値とすることで,気温データのみから氷板厚を計算可能であることを示した.
- Published
- 2012
- Full Text
- View/download PDF
17. The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3
- Author
-
Junko Kominami, Takako Naganuma, Sachiko Nakamura-Tsuruta, Yasuharu Watanabe, Jun Hirabayashi, Hiroaki Tateno, Ryno J. Naudé, Osamu Nakamura, Hisao Kamiya, Koji Muramoto, Tomohisa Ogawa, and Tasuku Watanabe
- Subjects
endocrine system ,Phagocytosis ,Immunology ,Biology ,Rhamnose ,Antibodies ,Cell Line ,Substrate Specificity ,Proinflammatory cytokine ,Immunity ,Lectins ,Animals ,Antigens, Tumor-Associated, Carbohydrate ,Opsonin ,Phagocytes ,Innate immune system ,urogenital system ,Cell Membrane ,Lectin ,Rhamnose binding ,Immunity, Innate ,Oncorhynchus keta ,Gene Expression Regulation ,Biochemistry ,Cell culture ,Oncorhynchus mykiss ,biology.protein ,Cytokines ,Protein Binding ,Developmental Biology - Abstract
L-rhamnose-binding lectins (RBLs) have been isolated from various kinds of fish and invertebrates and interact with various kinds of bacteria, suggesting RBLs are involved in various inflammatory reactions. We investigated the effect of RBLs from chum salmon (Oncorhynchus keta), named CSL1, 2 and 3, on the peritoneal macrophage cell line from rainbow trout (Oncorhynchus mykiss) (RTM5) and an established fibroblastic-like cell line derived from gonadal tissue of rainbow trout (RTG-2). CSLs were bound to the surface of RTM5 and RTG-2 cells and induced proinflammatory cytokines, including IL-1beta1, IL-1beta2, TNF-alpha1, TNF-alpha2 and IL-8 in both cells by recognizing globotriaosylceramide (Gb3). In addition, CSLs had an opsonic effect on RTM5 cells and this effect was significantly inhibited by L-rhamnose, indicating that CSLs enhanced their phagocytosis by binding to Gb3 on cell surfaces. This is the first finding that Gb3 plays a role in innate immunity by cooperating with natural ligands, RBLs.
- Published
- 2009
- Full Text
- View/download PDF
18. ANALYSIS OF DRIFTWOOD BEHAVIOR AND ACCUMULATIONS ON SANDBAR CONSIDERING FLOOD FLOW AND DISCHARGE
- Author
-
Yasuharu Watanabe and Hiroki Yabe
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Organic Chemistry ,Flow (psychology) ,Shoal ,Driftwood ,Biochemistry ,Current (stream) ,Flood flow ,Flow velocity ,Streamflow ,Geomorphology ,Geology ,Riparian zone - Abstract
Field surveys, experiments and numerical calculations were conducted to examine driftwood accumulation in a river channel, with respect to the existence of sandbars. We investigated the areas where driftwood accumulated and captured from river channel is observed in two rivers. It seemed that factors such as the existence of riparian woods, sandbar configurations, and sandbar locations relative to the river flow determined the points of driftwood accumulation. Based on the field survey results, hydraulic model experiments investigated the flow path of driftwood in model river with alternate sandbars. The analysis related to flood flow indicates that when the flow velocity and the water depth of the main current differ greatly from the flow velocity and the water depth of the flow that is away from the main current, the force of the main current acting on driftwood pushes the driftwood outward. And we clarified the relationship between discharge and the locations of driftwood capture, traps were built at different points on a sandbar.
- Published
- 2008
- Full Text
- View/download PDF
19. [Untitled]
- Author
-
Atsushi Takeda, Tomonori Shimada, Shin Ishikawa, Syogo Oshima, Takeshi Kiryu, Hiroshi Yokoyama, and Yasuharu Watanabe
- Subjects
Sediment ,Soil science ,Geology ,Bed load ,Communication channel - Published
- 2008
- Full Text
- View/download PDF
20. Isolation and characterization of l-rhamnose-binding lectin, which binds to microsporidian Glugea plecoglossi, from ayu (Plecoglossus altivelis) eggs
- Author
-
Hiroki Matsubara, Sachiko Nakamura-Tsuruta, Hisao Kamiya, Nobuyuki Shiina, Yasuharu Watanabe, Fuminori Shinozaki, Kazuhiro Sugahara, Junko Kominami, Hiroshi Yokoyama, Jun Hirabayashi, Tomohisa Ogawa, and Koji Muramoto
- Subjects
Fish Proteins ,Glycoconjugate ,Molecular Sequence Data ,Spores, Protozoan ,Immunology ,Rhamnose ,Glycolipid ,Affinity chromatography ,Lectins ,Animals ,Amino Acid Sequence ,Pathogen ,Phylogeny ,Ovum ,chemistry.chemical_classification ,biology ,Lectin ,Rhamnose binding ,Immunity, Innate ,chemistry ,Biochemistry ,Osmeriformes ,Microsporidia ,biology.protein ,Plecoglossus altivelis ,Glycoprotein ,Developmental Biology - Abstract
A rhamnose-binding lectin, named SFL, was isolated from the eggs of ayu (sweet fish, Plecoglossus altivelis) by affinity and ion-exchange chromatographies. SFL revealed 287 amino acid residues with 3 tandemly repeated domains, and contained 8 half-Cys residues in each domain. The lectin was shown to have a highly specific binding affinity to globotriaosylceramide (Gb3) by frontal affinity chromatography using 100 oligosaccharides. SFL was localized in several tissues and serum of both male and female ayu, such as gill, liver, ovary, testis, intestine, stomach, brain, kidney and serum. The lectin agglutinated the spores of the microsporidian Glugea plecoglossi, which is a pathogen of ayu. Although SFL bound to glycoproteins and glycolipids of G. plecoglossi spores, Gb3 could not be detected in either of them. The results suggest that SFL could interact with various glycoconjugates of pathogens to play a role in the adhesion of microorganisms invading in the body.
- Published
- 2008
- Full Text
- View/download PDF
21. ANALYSIS OF STREAM CHANNEL GEOMETRY AND PHYSIOGRAPHIC FORMATIVE PROCESS IN VALLEY FLATS
- Author
-
Hiroyasu Yasuda, Kazuyoshi Hasegawa, Takeshi Nogami, and Yasuharu Watanabe
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Flood myth ,Floodplain ,Hydraulics ,Organic Chemistry ,Fluvial ,Flow channel ,Geometry ,Terrain ,Biochemistry ,law.invention ,law ,Geomorphology ,Meandering channel ,Design flood ,Geology - Abstract
Disasters causedby torrential downpours have recently been increasing throughout ournation. Inparticular, there have been many cases of flood damage on small and medium-sized rivers with relatively small design flood discharge in valley flats. Specific instances of this type damage include that which occurred in Tochigi Prefecture's Yosasa River in 1998, the Appetsu River in Hokkaido in 2003. These disasters are characterized by the flow of flood waters that were not confined to the river channel and caused damage to many houses, roads and bridges. In this study, we examined a method for adopting double Fourier analysis, which is used for shape analysis of the results of experiments on sediment hydraulics, to assess the flow channel geometry of an actual valley flat. The originof the terrain of the valley flat was estimated based on the results of sediment hydraulic studies, including experiments on compound meandering channel anddouble-row bar. The potential for utilizing the estimate results to predict the flow of flood waters and measures to reduce the impact of disasters at times of large-scale flooding in a valley flat was then indicated.
- Published
- 2007
- Full Text
- View/download PDF
22. THE EFFECT OF THE INITIAL BED PERTURBTION TO THE SAND BAR FORMATION AND EVOLUTION PROCESS
- Author
-
Yasuyuki Shimizu, Sanjay Giri, Kensuke Kobayashi, and Yasuharu Watanabe
- Subjects
Physics ,geography ,geography.geographical_feature_category ,Random field ,Series (mathematics) ,Bar (music) ,Thermodynamic equilibrium ,Computation ,Organic Chemistry ,Perturbation (astronomy) ,Shoal ,Mechanics ,Biochemistry ,Amplitude ,Geotechnical engineering - Abstract
This paper presents investigations, performed to assess the effect of the initial bed perturbation on the sand bar formation and evolution process. Computations were conducted with two different kind of initial bed perturbations. In the series of first computations, the influence of the amplitude of random field of perturbations was tested. The time to reach an equilibrium state was found to be depending on the amplitude of the initial random bed perturbation. It was found that the larger the amplitude, the shorter the time to reach an equilibrium state. The second test was conducted with the initial bed perturbation in the form of a double row bar. During the first stage of the computation, double row bar type bed configurations were formed, however the modes of bed configurations appeared to be reduced and finally turned into single mode type bed configurations. Since the hydraulic condition of the numerical experiment is single-bar regime, this experiment corresponds to the transformation of bar conditions from the doublemodes to the single-mode during the flood in the natural rivers.
- Published
- 2007
- Full Text
- View/download PDF
23. IMPACT OF A MESH STRUCTURE ON SALINITY INTRUSION
- Author
-
Yasuharu Watanabe, Yasuhiro Yoshikawa, and Hiroyasu Yasuda
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Organic Chemistry ,Fishing ,Saline water ,Biochemistry ,Nutrient ,Oceanography ,Flow velocity ,Weir ,River mouth ,Ecosystem ,Porosity ,Geology - Abstract
The ecosystem in the tidal region of a river tends to be diverse because of seawater intrusion. Such an ecosystem is observed in the Abashiri River in eastern Hokkaido. The downstream end of Lake Abashiri is about 7 kilometers from the Abashiri river mouth. The lower layer of the lake is saline water that has flowed upstream from the sea. Dissolved nutrient salts from the saline water layer make the lake a good fishing ground. However, in recent years, the damage to fishery of blue tide happens frequently because the fresh water depth of the lake became shallow. One solution is based on the comb-shaped weir traps that are used for catching fish in Abashiri River. Mesh structures that simulate weir traps were used in laboratory experiments to study the relationship between mesh porosity and salinity intrusion velocity. The following findings were obtained: The salinity intrusion experiment showed that when the mesh porosity is low, the flow velocity decreases before the water reaches the structure, and that the flow velocity does not differ greatly between before and after passing through the structure.
- Published
- 2007
- Full Text
- View/download PDF
24. HYDRAULIC EXPERIMENTS ON CHANGING PROCESS OF BAR SHAPE IN SMALL DISCHARGE PERIOD
- Author
-
Tomonori Shimada, Hiroyasu Yasuda, and Yasuharu Watanabe
- Subjects
Nonlinear system ,Transverse plane ,Interference (communication) ,Bar (music) ,Organic Chemistry ,Limit (music) ,Process (computing) ,Wavenumber ,Geotechnical engineering ,Mechanics ,Biochemistry ,Geology ,Communication channel - Abstract
Hydraulic experiments were conducted to clarify a changing process of bar shape formed during flood at usual time. Double-row bar-like shape appeared and reaching a meandering style channel was observed. It became clear that the component which has wavenumber 1 and 0 in the transverse and longitudinal directions respectively with formation of a meandering style channel increases as a result of the wave number analysis of bed forms. Furthermore, although it was examination near the limit of theory, weak nonlinear analysis considering the interference between (1, 1) and (2, 2) components was carried out, and the same result as an experiment result was obtained.
- Published
- 2007
- Full Text
- View/download PDF
25. Inflammatory responses increase secretion of MD-1 protein
- Author
-
Toshihiko Kobayashi, Tatsuya Yamazaki, Richard Thomas Jennings, Miyako Tanaka, Isao Ichimonji, Akina Nakashima, Ikuko Yamai, Yoshinori Nagai, Hidekazu Takagi, Sanae Haga, Kensuke Miyake, Yoshihiro Ogawa, Naoko Morita, Takane Kikuchi-Ueda, Erdenezaya Odkhuu, Yasuharu Watanabe, Sachiko Akashi-Takamura, Takayoshi Suganami, Michitaka Ozaki, and Kiyoshi Takatsu
- Subjects
0301 basic medicine ,Male ,medicine.drug_class ,Immunology ,Mice, Transgenic ,Monoclonal antibody ,Dexamethasone ,Antigen-Antibody Reactions ,03 medical and health sciences ,Mice ,0302 clinical medicine ,In vivo ,Antigens, CD ,medicine ,Immunology and Allergy ,Animals ,Secretion ,Receptor ,chemistry.chemical_classification ,Inflammation ,Mice, Knockout ,Mice, Inbred BALB C ,Membrane Glycoproteins ,biology ,Cell Death ,Antibodies, Monoclonal ,General Medicine ,Molecular biology ,Mice, Inbred C57BL ,TLR2 ,030104 developmental biology ,Biochemistry ,chemistry ,Antigens, Surface ,biology.protein ,TLR4 ,Female ,Antibody ,Glycoprotein ,030215 immunology - Abstract
Radioprotective 105 (RP105) is a type I transmembrane protein, which associates with a glycoprotein, MD-1. Monoclonal antibody (mAb)-mediated ligation of RP105/MD-1 robustly activates B cells. RP105/MD-1 is structurally similar to Toll-like receptor 4 (TLR4)/MD-2. B-cell responses to TLR2 and TLR4/MD-2 ligands are impaired in the absence of RP105 or MD-1. In addition to RP105/MD-1, MD-1 alone is secreted. The structure of MD-1 shows that MD-1 has a hydrophobic cavity that directly binds to phospholipids. Little is known, however, about a ligand for MD-1 and the role of MD-1 in vivo To study the role of RP105/MD-1 and MD-1 alone, specific mAbs against MD-1 are needed. Here, we report the establishment and characterization of two anti-MD-1 mAbs (JR2G9, JR7G1). JR2G9 detects soluble MD-1, whereas JR7G1 binds both soluble MD-1 and the cell surface RP105/MD-1 complex. With these mAbs, soluble MD-1 was detected in the serum and urine. The MD-1 concentration was altered by infection, diet and reperfusion injury. Serum MD-1 was rapidly elevated by TLR ligand injection in mice. The quantitative PCR and supernatant-precipitated data indicate that macrophages are one of the sources of serum soluble MD-1. These results suggest that soluble MD-1 is a valuable biomarker for inflammatory diseases.
- Published
- 2015
26. A FIELD OBSERVATION OF FLOW VELOCITY IN TREES DURING A FLOOD
- Author
-
Youichi Ishida, Taku Igarasi, Kazuhiro Mitamura, Yasuharu Watanabe, Yousuke Aburakawa, Atushi Tamadate, and Nobuyuki Suzuki
- Subjects
Hydrology ,Tree (data structure) ,Flow conditions ,Flow velocity ,Flood myth ,Typhoon ,Organic Chemistry ,Flow (psychology) ,Tree density ,Biochemistry ,Geology ,Field observation - Abstract
We measured the cross-sectional flow velocities in the tree zone of the Otofuke River during a flood caused by Typhoon Etau in August 2003 and tried to clarify the flow conditions at that time. It was confirmed that the obtained flow pattern of cross sectional distribution sufficiently reproduced behavior of the flow velocity in the tree zone. The calculated average flow velocity in the tree zone was about 0.12 - 0.27 m/s, which was 1/15th that of the main stream, whose average velocity was 3 m/s without trees. Discharge from the vegetated section accounted for about 6% of total section. And it has become necessary to examine the existing methods of measuring tree density. Key Words: field observation, the flow velocity in tree zone, ADP, tree density, the Otofuke river
- Published
- 2006
- Full Text
- View/download PDF
27. [Untitled]
- Author
-
Keizo WATANABE, Futoshi NAKAMURA, Miki KOBAYASHI, Seiji YANAI, Takao YONEDA, Yasuharu WATANABE, Noboru MARUOKA, and Hiroyuki KITAYA
- Subjects
Ecology ,Civil and Structural Engineering - Published
- 2006
- Full Text
- View/download PDF
28. SURVEY OF VERTICAL FORMATION OF FLOW VELOCITY WITH RIPARIAN WOODS ON 2005-FLOOD IN BIBAI RIVER
- Author
-
Shuya Abe, Takashi Kuwamura, and Yasuharu Watanabe
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Flood myth ,Dead water ,Total flow ,Organic Chemistry ,Biochemistry ,Water level ,Cross section (physics) ,Flow velocity ,Section (archaeology) ,Geology ,Riparian zone - Abstract
Riparian wood area is assumed to be dead water section on study for flowing ability. But, calculation results of water level might not correspond with observations on medium-small scale rivers. We surveyed about vertical formation of flood flow velocity on the cross section including riparian wood area in Bibai River on August 22, 2005. As a result, about 27% of total flow quantity flowed down through the riparian woods on this survey section. And, formation of flow velocity on woods area almost agrees with the theoretical value. But, formation of flow velocity outside the woods is lower than the theoretical one.
- Published
- 2006
- Full Text
- View/download PDF
29. WEAKLY NONLINEAR ANALYSIS OF BAR MODE REDUCTION PROCESS
- Author
-
Yasuharu Watanabe
- Subjects
Materials science ,Bar (music) ,business.industry ,Hydraulic test ,High Energy Physics::Phenomenology ,Organic Chemistry ,Linear amplification ,Process (computing) ,Mode (statistics) ,Structural engineering ,Mechanics ,Biochemistry ,Nonlinear system ,High Energy Physics::Experiment ,business ,Reduction (mathematics) - Abstract
Various hydraulic test and numerical simulations have reported that under the condition in which doublerow bars initially form, the mode of bars is reduced and alternate bars are formed over time. In this study, weakly nonlinear analysis is performed in order to elucidate the interaction between the modes of double-row bars and alternate bars. A concept of the rate of linear amplification is introduced in to the weakly nonlinear analysis in order to enable a consistent expansion of alternate bars and double-row bars. This analysis succeeds in reproducing the characteristics of bar mode decrease process. According to this analysis, neither of both modes (alternate bars and double-row bars ) can grow up simultaneously.
- Published
- 2006
- Full Text
- View/download PDF
30. FIELD INVESTIGATION OF DISASTERS IN SRI LANKAN RIVERS CAUSED BY SUMATRA EARTHQUAKE TSUNAMI
- Author
-
Kazuyoshi Hasegawa, Shinichiro Yano, Bandara Nawarathna, Yasuharu Watanabe, Hiroyasu Yasuda, Kazuo Ishino, Hajime Nakagawa, and Hitoshi Tanaka
- Subjects
Hydrology ,River engineering ,Emergency management ,Hydraulics ,business.industry ,Hydraulic engineering ,Organic Chemistry ,Survey result ,Biochemistry ,law.invention ,Indian ocean ,Geography ,law ,Forensic engineering ,Damages ,Sri lanka ,business - Abstract
The magnitude 9.0 2004 Indian Ocean Earthquake triggered a series of lethal tsunamis on December 26, 2004 that killed over 225, 000 people, making it the deadliest tsunami in recorded history. In order to investigate this tsunami and related damages from the hydrological and hydraulic aspects, the Committee on Hydroscience and Hydraulic Engineering, Japan Society of Civil Engineers (JSCE) delegated its members whose majors are coastal hydraulics, river engineering, hydraulic engineering and hydrology as a tsunami investigation team by JSCE. In this paper, results of filed investigation are shown mainly for disasters in rivers. The present survey results will be useful for future planning of tsunami disaster prevention in Sri Lanka, as well as in Japan, where near future occurrence of tsunami disaster is highly expected.
- Published
- 2006
- Full Text
- View/download PDF
31. [Untitled]
- Author
-
Kensuke Kobayashi, Yasuharu Watanabe, and Yasuyuki Shimizu
- Subjects
Unsteady flow ,geography ,geography.geographical_feature_category ,Meteorology ,Shoal ,Mechanics ,Geology - Published
- 2006
- Full Text
- View/download PDF
32. SUITABILITY OF WEAKLY NONLINEAR ANALYSIS TO MODE REDUCTION PROCESS OF DOUBLE-ROW BARS
- Author
-
Takashi Kuwamura and Yasuharu Watanabe
- Subjects
Bar (music) ,business.industry ,Organic Chemistry ,Mode (statistics) ,Process (computing) ,Perturbation (astronomy) ,Mechanics ,Structural engineering ,Biochemistry ,Nonlinear system ,Wavelength ,Growth rate ,Reduction (mathematics) ,business ,Mathematics - Abstract
Weakly nonlinear analysis was adapted to the hydraulic experiments in order to clarify the mechanisms of mode reduction process of sand bars. Hydraulic conditions of this analysis were set large width-depth ratio. The following facts became clear by this research. Initial bar growth rate must be calculated precisely when the mode reduction process is considered. The bar wavelength which passes through the mode reduction process of bar formation can be calculate by the linear stability analysis. The weakly nonlinear analysis can predict the mode reduction process of bars when the perturbation parameter is less than 1. This analysis shows good agreement on the contribution of each bar mode element to the bed formation.Although the growth rate expansion method has a mathematical problem applying to the multiple bar formation process, this analysis shows good agreement on the hydraulic experimental results.
- Published
- 2005
- Full Text
- View/download PDF
33. AN OUTLINE OF HEAVY RAINFALL DISASTERS IN HIDAKA REGION, HOKKAIDO, BY TYPHOON NO.10, 2003
- Author
-
Toshiko Yamashita, Yasunori Watanabe, Yasuhiro Murakami, Hiroshi Saga, Mutsuhiro Fujita, Osamu Shimizu, Satoshi Tohma, Tohru Arayae, Hiroyuki Suzuki, Mikio Kuroki, Makoto Nakatsugawa, Shigemi Hatta, Gaku Tanaka, Kazuyoshi Hasegawa, Takashi Yamada, Shunichi Kikuchi, Yuichi Suzuki, Hitoshi Tanaka, Toshimitsu Komatsu, Takehiro Ogawa, and Yasuharu Watanabe
- Subjects
Slope failure ,Hydrology ,geography ,Missing person ,geography.geographical_feature_category ,Flood myth ,Floodplain ,Typhoon ,Organic Chemistry ,Drainage basin ,Driftwood ,Biochemistry - Abstract
The typhoon No.10 hit the Pacific cost in Hokkaido on August 9 in 2003. The casualties were 10 killed and 3 wounded. The missing person has not been found yet. This typhoon gave the biggest damage by flood to Hokkaido in the last twenty years. In Saru River basin and Appetu River basin in Ilidaka region, serious damages, such as flooded house, house failure and bridge fall, occurred by this typhoon. Farmland and flood plain were covered for the large sediment.In this paper, we will report an outline of heavy rainfall disasters of Hidaka region in Hokkaido by the typhoon No.10. We hope that the result of this investigation helps to clarify the mechanisms of the heavy rainfall disasters with hydrologic and hydraulic knowledge.
- Published
- 2005
- Full Text
- View/download PDF
34. SEDIMENT TRANSPORT IN THE NIBUTANI DAM RESERVOIR AT 2003 FLOOD LF THE SARU RIVER
- Author
-
Yasuhiro Yosikawa, Tomonori Shimada, and Yasuharu Watanabe
- Subjects
Hydrology ,Flood myth ,Organic Chemistry ,River management ,Sediment ,Sedimentation ,Entrainment (chronobiology) ,Biochemistry ,Sediment transport ,River bed ,Geology - Abstract
Sedimentation in dam reservoirs is a serious matter of great concern with river management works. In general, sediment deposited in the reservoir is hard to move comparing with river bed materials, even if the diamete is equal. Therefore, hydraulic experiments were conducted on the fall velocity of sediment and the entrainment rate of suspended sediment using the material which is deposited in the dam reservoir. It is found that the fall velocity of sediment and the entrainment rate of suspended sediment are smallre then the value currently used conventionally. These results are applied to calculate bed variation of the 2003 flood in the Nibutani Dam resevoir. The calculated results mostly agree with field surveys.
- Published
- 2005
- Full Text
- View/download PDF
35. [Untitled]
- Author
-
Yasuharu WATANABE, Kazuyoshi HASEGAWA, Akio MORI, and Yuichi SUZUKI
- Subjects
Ecology ,Civil and Structural Engineering - Published
- 2005
- Full Text
- View/download PDF
36. FLOW VELOCITY CALCULATED FROM UPROOTED TREES AT THE AUGUST 2003 FLOOD OF SARU RIVER
- Author
-
Yasuharu Watanabe, Yousuke Aburakawa, and Shuya Abe
- Subjects
Hydrology ,Flood myth ,Flow velocity ,Organic Chemistry ,Driftwood ,Biochemistry ,Geomorphology ,Geology - Published
- 2005
- Full Text
- View/download PDF
37. REPORT ON RIVER-RUNUP OF TSUNAMI GENERATED BY THE TOKACHI-OKI EARTHQUAKE ON 26 SEPTEMBER, 2003
- Author
-
Yasuharu Watanabe, Hiroyasu Yasuda, and Koji Fujima
- Subjects
Geology ,Seismology - Published
- 2004
- Full Text
- View/download PDF
38. FIELD OBSERVATIONS OF THE AUGUST 2003 FLOOD IN THE SARU RIVER BASIN
- Author
-
Takehiro Ogawa and Yasuharu Watanabe
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Floodplain ,Flood myth ,Organic Chemistry ,Flooding (psychology) ,Water storage ,Drainage basin ,Sediment ,Biochemistry ,Flood control ,Sediment transport ,Geology - Abstract
Recent problems facing river management include a decrease in the water storage capacity of dam reservoirs due to sedimentation and a lack of continuity in sediment moment from the upper to lower reaches. To clarify the characteristics of substance transported during flooding, we made observations of and collected samples from the Saru River in Hokkaido, which has a reservoir in its middle reaches. In this area, a massive flood occurred between August 9 and 11, 2003. We also observed this particular flood. Since the scale of this flood was very large, we were unable to make observations throughout the duration of the flood. We were, however, able to conduct observations during the first half of the flood and at the end of the flood end. Here we report our observation results.
- Published
- 2004
- Full Text
- View/download PDF
39. RIVER BED CHANGE OF THE TOKACHI RIVER IN FLOOD, AUGUST 2003
- Author
-
Takashi Kuwamura and Yasuharu Watanabe
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Flood myth ,Bar (music) ,Organic Chemistry ,Flow (psychology) ,Biochemistry ,Peak water ,River bed ,Field observation ,Unsteady flow ,Geomorphology ,Geology ,Channel (geography) - Abstract
Field observation of bed configuration was conducted during the 2003 August flood at the Tokachi River. Radio controlled boat was used in this measurement. The aim of this observation is to clarify the change of bed topography during flood. It is found that the bar formation becomes unclear and the flow becomes straight along the channel at the neighborhood of peak water level in which the hydraulic condition becomes no bar region. The changing of observed bed configuration during the flood is compared with the result of stability analysis under unsteady flow condition. The general tendencies of bar formation change by the theoretical results agree with field data. However, the theoretical results cannot reproduce the observed bar wavelength.
- Published
- 2004
- Full Text
- View/download PDF
40. EXPERIMENTAL STUDY ON MODE REDUCTION PROCESS OF DOUBLE-ROW BARS
- Author
-
Takashi Kuwamuka and Yasuharu Watanabe
- Subjects
Materials science ,business.industry ,Organic Chemistry ,Mode (statistics) ,Process (computing) ,Double row ,Mechanics ,Sand bars ,Structural engineering ,Biochemistry ,Time changes ,Reduction (complexity) ,Single row ,Wavenumber ,business - Abstract
Hydraulic experiments were conducted in order to clarify the mechanisms of mode reduction process of sand bars. Hydraulic conditions of these experiments were set large width-depth ratio. The following facts became clear by these experiments. Starting time of the mode reduction becomes late and time to spend on the mode reduction has the tendency which becomes long, when a width-depth ratio becomes large. One of two scoured area near side walls in double-row bars is buried and single-row bars are formed.Furthermore, the prominence wave number of the bed form is analyzed. It is possible that the single row bars formed by mode reduction from double row bars are the same with the single row bars which are formed from the early stages of experiments on the bed form characteristics. Time changes of amplification of (α21+α40) and (α11+α20) have contributed greatly to the mode reduction process.
- Published
- 2004
- Full Text
- View/download PDF
41. BEHAVIOUR OF DRIFTWOOD IN THE SARU RIVER DURING TYPHOON NO.10
- Author
-
Yasuharu Watanabe and Yuichi Suzuki
- Subjects
Shore ,Hydrology ,geography ,geography.geographical_feature_category ,Floodplain ,Flood myth ,Typhoon ,Organic Chemistry ,Drainage basin ,Driftwood ,Biochemistry ,Geology - Abstract
Typhoon Na.10 attacked the Pacific side of Hokkaido on August 9, especially in Iburi and Hidaka sub prefectures. The flood generated large amounts of driftwood and caused bridge washout in the Saru River basin. Based on an onsite survey conducted immediately after the typhoon, we examined the driftwood in the Saru River. We studied the distribution of locations where driftwood beached in the river channel, the volume of such driftwood, the distribution of driftwood origin in the river channel, reasons for driftwood originating there. The influence of driftwood to the rivers structures (bridge piers etc) is also investigated. It became clear that the driftwood generated from the river channel and the mountain area occupied 20% and 40% of all driftwood, respectively as a result of investigation. The generating places of the driftwood from the river channel were the flood plain at the inner bend and the eroded outer bank shore.
- Published
- 2004
- Full Text
- View/download PDF
42. STUDY ON SIMULATION OF RIVERBED CHANGE FOR TWO-WAY CHANNEL RIVER
- Author
-
Yasuharu Watanabe, Hiroshi Yokoyama, and Yuichi Suzuki
- Subjects
Test flow ,Computer simulation ,Two way channel ,Section (archaeology) ,Organic Chemistry ,Flow (psychology) ,Range (statistics) ,Meander ,Geotechnical engineering ,Biochemistry ,Geology ,Communication channel - Abstract
A project for river-restoration on the Shibetsu River was launched in 2000. For meander restoration, test flow of water started in March 2002 in a bifurcated channel formed by connecting the pre-improved channel (oxbow) with the present straight channel. In the eighteen months that have elapsed, the riverbanks and riverbed in the test section have greatly changed.To analyze hydraulic phenomena under various conditions, this study aimed to develop a numerical calculation method that enables estimation of flow and riverbed evolution. We made calculations that reproduce riverbed evolution for the test section. The reproducibility was verified by comparing calculation results and measurements. We clarified the applicability and applicable range of the calculation method.
- Published
- 2004
- Full Text
- View/download PDF
43. CHANGING OF TWO-WAY RIVER COURSES IN RESTORATION PROJECT AT THE SHIBETSU RIVER
- Author
-
Yuichi Suzuki, Kazuyoshi Hasegawa, Yasuharu Watanabe, Takeshi Kuga, and Akio Mori
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Organic Chemistry ,Shoal ,Biochemistry ,Geology - Published
- 2003
- Full Text
- View/download PDF
44. EXPERIMENTAL STUDY ON BAR FORMATION UNDER UNSTEADY FLOW CONDITIONS USING A WIDE WIDTH CHANNEL
- Author
-
Takashi Kuwamura and Yasuharu Watanabe
- Subjects
Bar (music) ,High Energy Physics::Phenomenology ,Organic Chemistry ,Sediment ,Hydrograph ,Mechanics ,Biochemistry ,Physics::Fluid Dynamics ,Unsteady flow ,Flow conditions ,Linear stability analysis ,High Energy Physics::Experiment ,Geotechnical engineering ,Geology ,Bank erosion ,Communication channel - Abstract
In rivers that have sections of single-row bar and sections of double-row bar, the occurrence mechanisms of bank erosion, sediment accumulation, and other phenomena differ between these two types of section. To determine which of these sections, non-linear analysis of conditions over the border between the regions is needed.In this study, hydraulic experiments were conducted using a wide width channel in order to understand the bar formation process under unsteady flow conditions. Hydrograph of the experiments were set as the alternate bars and double-raw bars were generated. The results of experiments were compared with linear stability analysis under unsteady flow conditions. It was found that alternate bars and double-raw bars were generated under the influence of flow conditions process.
- Published
- 2003
- Full Text
- View/download PDF
45. QUANTITATIVE CLASSIFICATION OF TOPOGRAPHICAL FEATURES OF STEEP-STREAM RIVERBEDS
- Author
-
Makoto Nakatsugawa, Yasuharu Watanabe, and Takeshi Nogami
- Subjects
Hydrology ,Hydraulics ,Organic Chemistry ,Sediment ,STREAMS ,Field survey ,Biochemistry ,Quantitative classification ,law.invention ,Benthos ,Habitat ,law ,%22">Fish ,Environmental science - Abstract
Relationships between variations in river topography, such as riffles and pools, and fish, benthos, etc., have long been studied. The topographical classification of riffles and pools, however, is still conducted qualitatively. To take measures for the restoration or improvement of habitats, it is necessary to quantify these relationships and explain them in hydraulic terms. Meanwhile, studies on river -bed morphology have been conducted from the sediment hydraulics viewpoint, allowing for expression with physical indices. This paper examines the quantitative classification of riffles and pools of mountain streams, based on the findings of sediment hydraulics, to clarify their characteristics.
- Published
- 2003
- Full Text
- View/download PDF
46. BIFURCATED FLOW RATE TO THE OXBOW MEANDER CHANNEL REGULATED BY A SMALL DAM IN THE SHIBETSU RIVER
- Author
-
Kazuyoshi Hasegawa, Masaki Fujita, Takashi Kuwamura, and Yasuharu Watanabe
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Organic Chemistry ,STREAMS ,Biochemistry ,River water ,Volumetric flow rate ,Current (stream) ,Meander ,Meandering channel ,Geology ,Channel (geography) ,Meander cutoff - Abstract
A project of river channel restoration has been promoted in the Shibetsu River, that attempts to restore the natural meander channel remained as a oxbow lake by letting the river water run through. In this project, it is intended that water streams into both the restored meandering channel and the straightened current river course by a small dam constructed downstream of the bifurcation point. But there are few papers treating the relationship between bifurcated flow ratio and the height of a dam in such complex channel system. This study is devoted to investigate the regulation function of a dam for bifurcated flow rate into an oxbow channel. Theoretical solutions for the flow rate are derived from the momentum balance of the several controlvolume sections of the bifurcated area. The results are compared with observed data in model tests and in field survey at a study section of the Shibetsu River to show good agreement.
- Published
- 2003
- Full Text
- View/download PDF
47. INVESTIGATION ON CONTENT OF PHOSPHOROUS ABSORBED IN SUSPENDED SEDIMENT TRANSPORTED TO THE COAST
- Author
-
Shin-ichi Yamazaki, Yasuharu Watanabe, and Toshihiko Yamashita
- Subjects
Hydrology ,Watershed ,Phosphorus ,Organic Chemistry ,Flooding (psychology) ,chemistry.chemical_element ,Sediment phosphorus ,Sediment ,Particle (ecology) ,Biochemistry ,chemistry ,parasitic diseases ,Environmental science ,Wash load ,Water quality ,geographic locations - Abstract
Conditions of watershed and rivers are closely connected with coastal environments. Suspended sediment is important to water quality on the coast. In particular, at times of flooding a large quantity of suspended sediment is transported to the coast, and the majority of phosphorus is transported in particle form rather than dissolved form. But not all sediment phosphorus circulates to the coast, for example apatite phosphorus. For this reason, a quantitative analysis on the chemical form of phosphorus was conducted. Suspended sediment was sampled during flooding of the Ishikari, Shiribetsu and Mukawa rivers, and the phosphorus chemical combined forms of the samples were analyzed. The results show that more than 60% of the phosphorous in the suspended sediment is circulating phosphorous, and that the total phosphorous content is related to the suspended sediment diameter and is different for each river according to land use.
- Published
- 2003
- Full Text
- View/download PDF
48. RESEARCH ON CLASSIFICATION OF HABITAT IN MOUNTAIN RIVERS
- Author
-
Takeshi Nogami, Yasuharu Watanabe, and Kazuyoshi Hasegawa
- Subjects
Hydrology ,Riffle ,Bedform ,geography.geographical_feature_category ,Hydraulics ,Organic Chemistry ,Sediment transportation ,Biochemistry ,Natural (archaeology) ,law.invention ,Geography ,Habitat ,law ,Natural disaster ,Channel (geography) - Abstract
Mountain rivers are very steep and sediment transportation occurs frequently. It is very difficult to prevent natural disaster on natural mountain rivers, whose forms tend to change. Therefore river works which had an impact on the environment, for example channel work, have been constructed. But mountain rivers have many pools, riffle and ecological variety. So we believe we can restore, rehabilitate and protect the river eco-system by using natural hydraulics features of mountain rivers. This report describes classification of habitat by longitudinal section. And it also describes relation between medium-scale bedforms.
- Published
- 2002
- Full Text
- View/download PDF
49. DEPOSITION IN MAIN CHANNELS AND SCOURING ON FLOODPLAINS CAUSED BY SAND BARS DURING FLOODS
- Author
-
Fumiaki Ooyama and Yasuharu Watanabe
- Subjects
Hydrology ,geography ,geography.geographical_feature_category ,Floodplain ,Flood myth ,Organic Chemistry ,Shoal ,Sand bars ,Biochemistry ,Revetment ,Shear stress ,Geotechnical engineering ,Deposition (chemistry) ,Geology ,Communication channel - Abstract
Large-scale scouring was generated by the 1998 flood of the Shokotsu River resulting in revetment damage. The main channel was filled up by deposition during the 1981 flood of the Toyohira River. The cause of this scouring of the floodplain and deposition in main channel was considered for the purpose of understanding the impact of flood to channels. It seemed that the scouring and deposition was caused by the formation of double-row bars. In general, the nondimensional shear stress at the floodplain is smaller than at the main channel. Therefore, it seems that the floodplain is hard to be scoured. However, when the elevations of floodplain surface and main channel bed are not so different, a lot of deposition and scouring in channel is caused by multi-row bars during large scale flood. This phenomenon is shown in this paper using actual river data.
- Published
- 2002
- Full Text
- View/download PDF
50. EXPERIMENTAL STUDY ON BAR FORMATION UNDER UNSTEADY FLOW CONDITIONS
- Author
-
Koji Sato, Fumiaki Oyama, and Yasuharu Watanabe
- Subjects
Physics::Fluid Dynamics ,Wavelength ,Flow conditions ,Bar (music) ,Organic Chemistry ,Flow (psychology) ,Wave height ,Geotechnical engineering ,Stage (hydrology) ,Falling (sensation) ,Biochemistry ,Geology ,Water level - Abstract
In the previous researches on the sand bed stability with finite amplitude and growth of alternate bars, it is assumed that flow changes over time due to the unsteady flow do not affect the generation of alternate bars. Hydraulic experiments on the formation of alternate bars under unsteady flow conditions are conducted in this paper. The wavelength and wave height of alternate bars differ significantly between unsteady and steady flow conditions and between rising and falling stage of the water level due to the flood wave. Unsteadiness is typical of actual formative conditions for alternate bars, especially for gravel bed rivers. Results suggest that finite amplitude effects of the flood-wave on alternate bar formation are not negligible when investigating their behavior under unsteady flow conditions.
- Published
- 2002
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.