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29 results on '"Yao, Wei-Feng"'

6. Alternative processing technology for the preparation of carbonized Zingiberis Rhizoma by stir-frying with sand

Author

Shen Mei-Yu, Wang Jia-Li, Shi Hai-Pei, Yan Hui, Chen Pei-Dong, Yao Wei-Feng, Bao Bei-Hua, and Zhang Li

Subjects
stir-frying processing technology, fingerprint, gingerols, shogaols, Therapeutics. Pharmacology, RM1-950
Abstract

Context Carbonized ginger, a type of charry herb, has been used as a hemostatic medicine since ancient times. However, there are some serious problems such as inhomogeneous heating and emitting smoke during processing with traditional stir-frying method. Objective To investigate the feasibility to obtain carbonized ginger by stir-frying with sand instead of stir-frying method. Materials and methods Dried-ginger (100 g) was processed by stir-frying for 30 min at 270 ± 10 °C, or by stir-frying with sand (1:10, w/w) for 8 min at 240 ± 5 °C. The HPLC fingerprint was established for two samples. The adsorption capacity and major components including tannins, gingerols, shogaols and gingerone were quantitated by UV and HPLC, respectively. The hemostatic effect by prothrombin time (PT) and activated partial thromboplastin time (APTT) was evaluated in vitro. Results The similarity of the two samples for HPLC fingerprints was >0.93. The sand-fried samples showed significantly higher adsorption capacity compared with the stir-fried samples (4.915 vs. 4.593 mg/g; p 0.05). The PT and APTT values were similar between the stir-fried and sand-fried test groups and significantly lower compared to controls (p

Published
2020
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10. Impact of metabolic syndrome on the long-term prognosis of patients with hepatitis B virus-related hepatocellular carcinoma after hepatectomy

Author

Zhang, Kang-Jun, primary, Ye, Tai-Wei, additional, Lu, Wen-Feng, additional, Xu, Fei-Qi, additional, Xie, Ya-Ming, additional, Wang, Dong-Dong, additional, Xiao, Zun-Qiang, additional, Liu, Si-Yu, additional, Yao, Wei-Feng, additional, Cheng, Jian, additional, Shen, Guo-Liang, additional, Liu, Jun-Wei, additional, Zhang, Cheng-Wu, additional, Huang, Dong-Sheng, additional, and Liang, Lei, additional

Published
2022
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12. Association of preoperative albumin–bilirubin with surgical textbook outcomes following laparoscopic hepatectomy for hepatocellular carcinoma

Author

Xu, Fei-Qi, primary, Ye, Tai-Wei, additional, Wang, Dong-Dong, additional, Xie, Ya-Ming, additional, Zhang, Kang-Jun, additional, Cheng, Jian, additional, Xiao, Zun-Qiang, additional, Liu, Si-Yu, additional, Jiang, Kai, additional, Yao, Wei-Feng, additional, Shen, Guo-Liang, additional, Liu, Jun-Wei, additional, Zhang, Cheng-Wu, additional, Huang, Dong-Sheng, additional, and Liang, Lei, additional

Published
2022
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18. MiR-200a inhibits cell proliferation and EMT by down-regulating the ASPH expression levels and affecting ERK and PI3K/Akt pathways in human hepatoma cells

Author

Yao, Wei-Feng, Liu, Jun-Wei, and Huang, Dong-Sheng

Subjects
Original Article
Abstract

The primary objective of this study was to investigate the role of miR-200a in cell proliferation and epithelial-mesenchymal transition (EMT) through regulating targeting aspartate-β-hydroxylase (ASPH), which may further affect the activation of ERK/PI3K/Akt pathway. Liver cancer and adjacent tissues were collected from 72 cases of liver cancer patients with surgery in our hospital. In this study, the mRNA expression level of miR-200a was significantly decreased by real-time PCR (RT-PCR) detection. ASPH expressions, however, had an opposite tendency compared to that of miR-200a. We found a significantly negative correlation between miR-200a expressions and ASPH expressions. The survival rate of liver cancer patients with the low expressed ASPH was significantly higher than those with the high expressed ASPH. RT-PCR and Western blot results showed that low expressed miR-200a and highexpressed ASPH were found in liver cancer cell lines. Further research discovered that miR-200a transfection could significantly decrease the relative luciferase activity when it was integrated with ASPH 3'-untranslated region (3'-UTR) in HepG2 cells. Cell Counting Kit (CCK-8) detection showed that treatment with miR-200a mimics reduced cell viability, while the over-expressed ASPH increased cell viability by regulating the c-mycmrna (c-Myc) and Cyclin-D1 expressions. The EMT-related genes including E-Cadherin, N-Cadherin and Vimentin expressions were significantly increased, whereas the over-expressed ASPH exerted the opposite effects. In addition, extracellular signal regulated kinase (ERK), phosphoinositide-3-kinase (PI3K) and serine threonine kinase (AKT) were suppressed by miR-200a mimics. In conclusion, miR-200a inhibits cell proliferation and EMT in human hepatoma cells by targeting ASPH and affecting ERK and PI3K/Akt signaling pathways.

Published
2018

20. Atherosclerosis and Helminths Infection

Author

Shen, Shi-Wei, Lu, Yun, Li, Feng, Shen, Zhen-Hai, Xu, Ming, Yao, Wei-Feng, Qi, Hua-Jin, Zhou, Ling, Feng, Yin-Bo, Wang, Ling, and Tong, Jing-Ting Yun and Da-Xin

Published
2017

24. Chemical Constituents from Euphorbia kansui

Author

Zhang, Qiao, primary, Zhou, Qin-Rong, additional, Lou, Jian-Wei, additional, Chen, Pei-Dong, additional, Yao, Wei-Feng, additional, Tao, Wei-Wei, additional, Tang, Yu-Ping, additional, Dai, Guan-Cheng, additional, Wang, Kun, additional, and Zhang, Li, additional

Published
2017
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28. Preparation of Polyhedral Copper Oxide Nanoparticles by Molten-salt Method and Their Catalytic Performance

Author

Bai Yang, Dong Xian-Lin, Li Hao, Yao Wei-Feng, and Zeng Tao

Subjects
Copper oxide, Materials science, Inorganic chemistry, Nanoparticle, Catalysis, Nanomaterials, Inorganic Chemistry, Crystallinity, chemistry.chemical_compound, chemistry, Specific surface area, Rhodamine B, General Materials Science, Hydrogen peroxide
Abstract

Polyhedral copper oxide (CuO) nanoparticles were successfully prepared by a molten-salt method without any surfactant or template. Scanning electron microscopy images of the copper oxide samples demonstrated that the as-prepared nanoparticles have a polyhedral structure. The catalytic performances of copper oxide on the degradation of Rhodamine B in the presence of hydrogen peroxide were investigated. The results show that the polyhedral copper oxide nanoparticles exhibited much better catalytic performance in the degradation of Rhodamine B with hydrogen peroxide than commercial nano particles, even with smaller specific surface area. The possible reason can be attributed to the synergistic effect of high crystallinity and the exposed facets formed by polyhedral structure.

Published
2015

29. Toxicity Reduction of Euphorbia kansui Stir-Fried with Vinegar Based on Conversion of 3- O -(2' E ,4' Z -Decadi-enoyl)-20- O -acetylingenol.

Author

Zhang Q, Zhang Y, Zhou SK, Wang K, Zhang M, Chen PD, Yao WF, Tang YP, Wu JH, and Zhang L

Subjects
Animals, Apoptosis drug effects, Caspase 3 metabolism, Caspase 9 metabolism, China, Chromatography, High Pressure Liquid, Diterpenes chemistry, Diterpenes pharmacology, Diterpenes toxicity, Drugs, Chinese Herbal chemistry, Euphorbia chemistry, Glutathione chemistry, Glutathione metabolism, Interleukin-2 metabolism, Interleukin-8 metabolism, Malondialdehyde chemistry, Malondialdehyde metabolism, Mass Spectrometry, Plant Roots chemistry, Succinate Dehydrogenase metabolism, Superoxide Dismutase metabolism, Zebrafish embryology, Zebrafish immunology, Zebrafish metabolism, Acetic Acid chemistry, Diterpenes metabolism, Euphorbia toxicity
Abstract

The dried roots of Euphorbia kansui S.L.Liou ex S.B.Ho have long been used to treat edema in China. However, the severe toxicity caused by Euphorbia kansui (EK) has seriously restricted its clinical application. Although EK was processed with vinegar to reduce its toxicity, the detailed mechanisms of attenuation in toxicity of EK stir-fried with vinegar (VEK) have not been well delineated. Diterpenoids are the main toxic ingredients of EK, and changes in these after processing may be the underlying mechanism of toxicity attenuation of VEK. 3- O -(2' E ,4' Z -decadienoyl)-20- O -acetylingenol (3-O-EZ) is one of the diterpenoids derived from EK, and the content of 3-O-EZ was significantly reduced after processing. This study aims to explore the underlying mechanisms of toxicity reduction of VEK based on the change of 3-O-EZ after processing with vinegar. Based on the chemical structure of 3-O-EZ and the method of processing with vinegar, simulation experiments were carried out to confirm the presence of the product both in EK and VEK and to enrich the product. Then, the difference of peak area of 3-O-EZ and its hydrolysate in EK and VEK were detected by ultra-high-performance liquid chromatography (UPLC). Furthermore, the toxicity effect of 3-O-EZ and its hydrolysate, as well as the underlying mechanism, on zebrafish embryos were investigated. The findings showed that the diterpenoids (3-O-EZ) in EK can convert into less toxic ingenol in VEK after processing with vinegar; meanwhile, the content of ingenol in VEK was higher than that of EK. More interestingly, the ingenol exhibited less toxicity (acute toxicity, developmental toxicity and organic toxicity) than that of 3-O-EZ, and 3-O-EZ could increase malondialdehyde (MDA) content and reduce glutathione (GSH) content; cause embryo oxidative damage by inhibition of the succinate dehydrogenase (SDH) and superoxide dismutase (SOD) activity; and induce inflammation and apoptosis by elevation of IL-2 and IL-8 contents and activation of the caspase-3 and caspase-9 activity. Thus, this study contributes to our understanding of the mechanism of attenuation in toxicity of VEK, and provides the possibility of safe and rational use of EK in clinics.

Published
2019
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