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Reconstruction of hydrocarbon generation history is essential to understanding the petroleum system. In this study, basin modeling was employed to investigate the primary source rocks in the Fushan Depression (FD), a significant oil-bearing basin situated in the South China Sea. The research findings indicate that different tectonic zones within the FD underwent distinct hydrocarbon generation stages. The step-fault zone and the central sag zone experienced one hydrocarbon generation stage at 10–0 Ma and 30–0 Ma, respectively. The slope zone, on the other hand, experienced two hydrocarbon generation stages, 40–23.5 Ma and 10–0 Ma, controlled by tectonic movements and heat flow variations. Furthermore, critical times for the process of the petroleum system have been determined based on this work and previous literature. The slope zone in the eastern FD is considered a favorable area for conventional hydrocarbon exploration due to the high maturity of source rocks promoted by volcanic heating and two significant oil charges. The central sag zone is identified as an excellent prospect for unconventional resources because of the substantial retention of hydrocarbons in in-source unconventional reservoirs long after hydrocarbon generation. These findings provide a valuable guide for further exploration. [ABSTRACT FROM AUTHOR]

Based on the dynamic characteristics of electric vehicles, this study describes the use of existing basic parameters of a specific electric vehicle to optimize the performance parameters of an asynchronous motor. In addition, a theoretical reference of such an asynchronous motor is provided. [ABSTRACT FROM AUTHOR]

Background: Orf, also known as contagious ecthyma (CE), is an acute, contagious zoonotic disease caused by the orf virus (ORFV). The F1L protein is a major immunodominant protein on the surface of ORFV and can induce the production of neutralizing antibodies., Methods: The prokaryotic expression system was used to produce the recombinant F1L protein of ORFV, which was subsequently purified and used to immunize mice. Positive hybridoma clones were screened using an indirect enzyme-linked immunosorbent assay (ELISA). The reactivity and specificity of the monoclonal antibody (mAb) were verified through Western blot and indirect immunofluorescence (IFA). The linear antigenic epitope specific to the mAb was identified through Western blot, using truncated F1L proteins expressed in eukaryotic cells. A multiple sequence alignment of the ORFV reference strains was performed to evaluate the degree of conservation of the identified epitope., Results: After three rounds of subcloning, a mAb named Ba-F1L was produced. Ba-F1L was found to react with both the exogenously expressed F1L protein and the native F1L protein from ORFV-infected cells, as confirmed by Western blot and IFA. The mAb recognized the core epitope 103 CKSTCPKEM 111 , which is highly conserved among various ORFV strains, as shown by homologous sequence alignment., Conclusion: The mAb produced in the present study can be used as a diagnostic reagent for detecting ORFV and as a basic tool for exploring the mechanisms of orf pathogenesis. In addition, the identified linear epitope may be valuable for the development of epitope-based vaccines., Competing Interests: HZ was employed by Xinyu Mengling Animal Husbandry Development Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhang, Zhang, Feng, Ba, Yang, Gong, Yang, Zhang, Sun and Li.)

Orf is a zoonosis caused by the Orf virus (ORFV), which is endemic in goats, sheep, and wild ruminants worldwide. Orf infection is prevalent in China, with outbreaks reported in several provinces. Currently, there is limited information available regarding the characterization of ORFV strains in Jiangxi province. This study investigated an acute outbreak of Orf that occurred in 2021 in a goat herd in the Jiangxi province of China. Clinical signs in this case included lesions on the lips, nose, and inside the mouth. The presence of ORFV was confirmed from tissue samples by polymerase chain reaction (PCR). The nucleotide sequences of the B2L and F1L genes were fully sequenced and used to construct phylogenetic trees. The results of this investigation identified the ORFV JXxy2021 as the cause of the outbreak. The phylogenetic analysis revealed that the ORFV strain JXxy2021 had the highest similarity to the ORFV strains GO and FJ-SL from the neighboring province of Fujian. This suggests that JXxy2021 was likely transmitted from Fujian province. The results have provided valuable information on the genetic characteristics of JXxy2021 and the endemic situations of Orf in China., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhang, Zhang, Meng, Feng, Gong, Yang, Yang, Xu, Zheng and Li.)