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Periprosthetic infection and mechanical loosening are two leading causes of implant failure in orthopedic surgery that have devastating consequences for patients both physically and financially. Hence, advanced prostheses to simultaneously prevent periprosthetic infection and promote osseointegration are highly desired to achieve long-term success in orthopedics. In this study, we proposed a multifunctional three-dimensional printed porous titanium alloy prosthesis coated with imidazolium ionic liquid. The imidazolium ionic liquid coating exhibited excellent bacterial recruitment property and near-infrared (NIR) triggered photothermal bactericidal activity, enabling the prosthesis to effectively trap bacteria in its vicinity and kill them remotely via tissue-penetrating NIR irradiation. In vivo anti-infection and osseointegration investigations in infected animal models confirmed that our antibacterial prosthesis could provide long-term and sustainable prevention against periprosthetic infection, while promoting osseointegration simultaneously. It is expected to accelerate the development of next-generation prostheses and improve patient outcomes after prosthesis implantation.

Trifluridine (FTD) is an effective drug for advanced colorectal cancer (CRC); however, the molecular mechanisms underlying FTD resistance has not been elucidated. This study aimed to explore mRNAs and microRNAs (miRNAs) associated with FTD resistance in CRC. The analyzed data were downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed- mRNAs (DEGs) and miRNAs (DEMs) between FTD-resistant and parental cell lines were screened. Next, the DEGs-related miRNAs were predicted and then DEGs-DEMs network was constructed, followed by functional enrichment analysis and protein–protein interaction (PPI) analysis. Meanwhile, the gene–drug interactions were identified. Finally, the prognostic significance of key genes was evaluated using survival analysis. A total of 23 DEMs and 928 DEGs were screened between FTD-resistant and CRC parental cells. The DEMs-DEGs network revealed that miRNAs such as hsa-miR-3714 and hsa-miR-3678-3p with higher degrees, and PPI network indicated that genes like HDAC5, ETV6, CCNF, SREBF1, and LDLR might be recognized as hub genes. Moreover, we observed that CCNF, HDAC5, ETV6, and SREBF1 were associated with the overall survival or disease-free survival of patients with CRC. These findings might provide an insight into potential therapeutic targets and druggable genes for FTD-resistant in patients with CRC.