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2. Genetic parameters of Black Soldier Flies estimated in full sib design

Author

Bouwman, A.C., Nugroho, Jovano Erris, Wongso, D., van Schelt, J., Pannebakker, B.A., Zwaan, B.J., Ellen, E.D., Bouwman, A.C., Nugroho, Jovano Erris, Wongso, D., van Schelt, J., Pannebakker, B.A., Zwaan, B.J., and Ellen, E.D.

Abstract

The aim of this study was to design a scheme for black soldier flies (BSF, Hermetia illuscens) to collect data and estimate genetic parameters for the recorded traits. A full sib family design was implemented in a practically feasible way to record multiple traits on the same individuals across a number of families. Genetic parameters were estimated using a linear mixed model. Heritability estimates for body weight at prepupa, body weight at pupa, development time to prepupa was 0.25, 0.45 and 0.38, respectively. The common environmental effect was large at the larval stage and reduced after housing the BSF individually. In general, body weight showed a positive phenotypic correlation between the S2, prepupa and pupa stages, and development time was positively correlated with body weight at (pre)pupa stage. In conclusion, a full sib family design is a practically feasible way to collect data for estimation of genetic parameters in the BSF.

Published
2022

3. Ofatumumab in relapsed nodular lymphocyte-predominant Hodgkin lymphoma: results of a phase II study from the German Hodgkin study group

Author

Eichenauer, D. A., Goergen, H., Pluetschow, A., Wongso, D., Behringer, K., Kreissl, S., Thielen, I., Halbsguth, T., Broeckelmann, P. J., Fuchs, M., Boell, B., von Tresckow, B., Borchmann, P., Engert, A., Eichenauer, D. A., Goergen, H., Pluetschow, A., Wongso, D., Behringer, K., Kreissl, S., Thielen, I., Halbsguth, T., Broeckelmann, P. J., Fuchs, M., Boell, B., von Tresckow, B., Borchmann, P., and Engert, A.

Published
2016

4. Ofatumumab in relapsed nodular lymphocyte-predominant Hodgkin lymphoma: results of a phase II study from the German Hodgkin study group

Author

Eichenauer, D A, primary, Goergen, H, additional, Plütschow, A, additional, Wongso, D, additional, Behringer, K, additional, Kreissl, S, additional, Thielen, I, additional, Halbsguth, T, additional, Bröckelmann, P J, additional, Fuchs, M, additional, Böll, B, additional, von Tresckow, B, additional, Borchmann, P, additional, and Engert, A, additional

Published
2015
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5. Final analysis of a randomized phase II trial with prednisone, vinblastine, doxorubicin, and gemcitabine in patients with early unfavorable Hodgkin lymphoma -PVAG-14 pilot

Author

Fuchs, M., Wongso, D., Pluetschow, A., Feuring-Buske, M., Hertenstein, B., Hoeffkes, H. -G, Vogelhuber, M., von Tresckow, B., Biersack, H., Link, H., Topp, M. S., Fischer, N., Bredenfeld, H., Sasse, S., Behringer, K., Boell, B., Borchmann, P., Engert, A., Fuchs, M., Wongso, D., Pluetschow, A., Feuring-Buske, M., Hertenstein, B., Hoeffkes, H. -G, Vogelhuber, M., von Tresckow, B., Biersack, H., Link, H., Topp, M. S., Fischer, N., Bredenfeld, H., Sasse, S., Behringer, K., Boell, B., Borchmann, P., and Engert, A.

Published
2014

6. FINAL ANALYSIS OF A RANDOMIZED PHASE II STUDY WITH PREDNISONE, VINBLASTINE, DOXORUBICIN, AND GEMCITABINE IN PATIENTS WITH EARLY UNFAVORABLE HODGKIN LYMPHOMA -PVAG-14 PILOT

Author

Fuchs, M., Wongso, D., Pluetschow, A., Feuring-Buske, M., Hertenstein, B., Hoeffkes, H. G., Vogelhuber, M., von Tresckow, B., Biersack, H., Link, H., Topp, M. S., Fischer, N., Bredenfeld, H., Sasse, S., Behringer, K., Boell, B., Borchmann, P., Engert, A., Fuchs, M., Wongso, D., Pluetschow, A., Feuring-Buske, M., Hertenstein, B., Hoeffkes, H. G., Vogelhuber, M., von Tresckow, B., Biersack, H., Link, H., Topp, M. S., Fischer, N., Bredenfeld, H., Sasse, S., Behringer, K., Boell, B., Borchmann, P., and Engert, A.

Published
2014

7. Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging.

Author

Harada K, Ito M, Wang X, Tanaka M, Wongso D, Konno A, Hirai H, Hirase H, Tsuboi T, and Kitaguchi T

Subjects
Amino Acid Sequence, Animals, Astrocytes metabolism, Calcium metabolism, Cyclic AMP chemistry, Luminescent Proteins chemistry, Models, Molecular, Molecular Conformation, Spectrophotometry, Structure-Activity Relationship, Red Fluorescent Protein, Biosensing Techniques, Cyclic AMP metabolism, Luminescent Proteins genetics, Luminescent Proteins metabolism, Molecular Imaging, Optogenetics methods
Abstract

cAMP is a common second messenger that is involved in various physiological processes. To expand the colour palette of available cAMP indicators, we developed a red cAMP indicator named "Pink Flamindo" (Pink Fluorescent cAMP indicator). The fluorescence intensity of Pink Flamindo increases 4.2-fold in the presence of a saturating dose of cAMP, with excitation and emission peaks at 567 nm and 590 nm, respectively. Live-cell imaging revealed that Pink Flamindo is effective for monitoring the spatio-temporal dynamics of intracellular cAMP generated by photoactivated adenylyl cyclase in response to blue light, and in dual-colour imaging studies using a green Ca 2+ indicator (G-GECO). Furthermore, we successfully monitored the elevation of cAMP levels in vivo in cerebral cortical astrocytes by two-photon imaging. We propose that Pink Flamindo will facilitate future in vivo, optogenetic studies of cell signalling and cAMP dynamics.

Published
2017
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