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Xiyi Wang,1 Jing Shao,2 Aozhou Weng,1 Jiaqi Lian,1 Fule Wen,1 Qiong Fang,1 Yun Hu1 1School of Nursing, Shanghai Jiao Tong University, Shanghai, 200025, People’s Republic of China; 2Institute of Nursing Research and Department of Nursing of Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, People’s Republic of ChinaCorrespondence: Yun Hu, School of Nursing, Shanghai Jiao Tong University, Shanghai, 200025, People’s Republic of China, Email huyunsy@shsmu.edu.cnBackground: The nursing workforce faces substantial challenges, particularly in the aftermath of the COVID-19 era. Developing an effective strategy for workforce maintenance and the strategic deployment of nurses is crucial.Purpose: This study aimed to explore and categorize nurses’ personality traits, with a focus on analyzing differences in their perceptions of the nursing work environment.Participants and Methods: Between January 2023 and February 2023, a multi-center cross-sectional study was carried out involving nurses from 12 tertiary hospitals actively engaged in frontline COVID-19 response duties. Through cluster sampling, surveys were distributed among eligible nursing staff, comprising a general information questionnaire, the Chinese Big Five Personality Questionnaire-Short Form, subjective evaluations of emergency nursing management, and the Chinese Nursing Work Environment Scale for Public Health Emergencies. Various statistical analyses, such as descriptive analysis, cluster analysis, non-parametric tests, and general linear model analysis, were employed to investigate the correlation between personality types and the perception of nursing work environments.Results: The analysis encompassed 1059 valid questionnaires, reflecting the experiences of frontline nurses. The majority of these nurses possessed 1– 5 years of experience, held junior professional titles, volunteered for their roles, and served as attending nu