Search

Your search keyword '"Wen-ji Geng"' showing total 4 results
Start Over Author "Wen-ji Geng" Search Limiters Full Text
4 results on '"Wen-ji Geng"'

1. Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate

Author

Na Han, Wen-ji Geng, Jian Li, Shu-ting Liu, Jie Zhang, Yi-jie Wen, Huai-hua Xu, Meng-yuan Li, Yan-ru Li, and Pei-pei Han

Subjects
T. mairei, transcriptome, taxol biosynthesis, cerium, MJ, Plant culture, SB1-1110
Abstract

Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out.

Published
2022
Full Text
View/download PDF

2. Improvement of Biomineralization of Sporosarcina pasteurii as Biocementing Material for Concrete Repair by Atmospheric and Room Temperature Plasma Mutagenesis and Response Surface Methodology

Author

Wen-Ji Geng, Ji-Long Yin, Pei-Pei Han, Li Mengnan, Shiru Jia, and Run-Ze Xue

Subjects
Calcite, Materials science, biology, Precipitation (chemistry), chemistry.chemical_element, General Medicine, Calcium, biology.organism_classification, Applied Microbiology and Biotechnology, Mineralization (biology), Sporosarcina pasteurii, chemistry.chemical_compound, Calcium carbonate, chemistry, Chemical engineering, Response surface methodology, Biotechnology, Biomineralization
Abstract

Microbially induced calcium carbonate precipitation (MICP) has recently become an intelligent and environmentally friendly method for the repair of cracks in concrete. In order to improve the ability of microbial materials to repair concrete cracks, we applied random mutagenesis and optimization of mineralization conditions to improve the quantity and crystal form of microbially precipitated calcium carbonate. Sporosarcina pasteurii ATCC 11859 was used as the starting strain to obtain the mutant with high urease activity by atmospheric and room temperature plasma (ARTP) mutagenesis. Then, the optimal biomineralization conditions and precipitation crystal form was studied using Plackett-Burman experimental design and response surface methodology (RSM). Biomineralization with 0.73 mol/L calcium chloride, 45 g/L urea, reaction temperature of 45°C, and reaction time of 22 h, significantly increased the amount of precipitated calcium carbonate, which was deposited in the form of calcite crystals. Finally, the repair of concrete using the optimized biomineralization process was evaluated. A comparison of water absorption and adhesion of concrete specimens before and after repairs showed that concrete cracks and surface defects could be efficiently repaired. This study provides a new method for the engineering of biocementing material for concrete repair.

Published
2021
Full Text
View/download PDF

3. Improvement of Biomineralization of

Author

Pei-Pei, Han, Wen-Ji, Geng, Meng-Nan, Li, Shi-Ru, Jia, Ji-Long, Yin, and Run-Ze, Xue

Subjects
Biomineralization, Analysis of Variance, Calcium Chloride, Sporosarcina, Plasma Gases, Construction Materials, Mutagenesis, Mutation, Temperature, Urea, Urease, Calcium Carbonate
Abstract

Microbially induced calcium carbonate precipitation (MICP) has recently become an intelligent and environmentally friendly method for repairing cracks in concrete. To improve on this ability of microbial materials concrete repair, we applied random mutagenesis and optimization of mineralization conditions to improve the quantity and crystal form of microbially precipitated calcium carbonate.

Published
2021

4. Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate.

Author

Na Han, Wen-ji Geng, Jian Li, Shu-ting Liu, Jie Zhang, Yi-jie Wen, Huai-hua Xu, Meng-yuan Li, Yan-ru Li, and Pei-pei Han

Subjects
JASMONATE, PACLITAXEL, SEQUENCE alignment, ANTINEOPLASTIC agents, CERIUM
Abstract

Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level. Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 mM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plantpathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results