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31 results on '"Watowich, Stanley J."'

3. A molecular signature defining exercise adaptation with ageing and in vivo partial reprogramming in skeletal muscle

Author

Jones, Ronald G., primary, Dimet‐Wiley, Andrea, additional, Haghani, Amin, additional, da Silva, Francielly Morena, additional, Brightwell, Camille R., additional, Lim, Seongkyun, additional, Khadgi, Sabin, additional, Wen, Yuan, additional, Dungan, Cory M., additional, Brooke, Robert T., additional, Greene, Nicholas P., additional, Peterson, Charlotte A., additional, McCarthy, John J., additional, Horvath, Steve, additional, Watowich, Stanley J., additional, Fry, Christopher S., additional, and Murach, Kevin A., additional

Published
2023
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6. Muscle-Specific Cellular and Molecular Adaptations to Late-Life Voluntary Concurrent Exercise

Author

Dungan, Cory M, primary, Brightwell, Camille R, additional, Wen, Yuan, additional, Zdunek, Christopher J, additional, Latham, Christine M, additional, Thomas, Nicholas T, additional, Zagzoog, Alyaa M, additional, Brightwell, Benjamin D, additional, VonLehmden, Georgia L, additional, Keeble, Alexander R, additional, Watowich, Stanley J, additional, Murach, Kevin A, additional, and Fry, Christopher S, additional

Published
2022
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9. Late‐life exercise mitigates skeletal muscle epigenetic aging.

Author

Murach, Kevin A., Dimet‐Wiley, Andrea L., Wen, Yuan, Brightwell, Camille R., Latham, Christine M., Dungan, Cory M., Fry, Christopher S., and Watowich, Stanley J.

Subjects
MUSCLE aging, RIBOSOMAL DNA, EXERCISE therapy, RECOMBINANT DNA, EPIGENETICS, RESISTANCE training
Abstract

There are functional benefits to exercise in muscle, even when performed late in life, but the contributions of epigenetic factors to late‐life exercise adaptation are poorly defined. Using reduced representation bisulfite sequencing (RRBS), ribosomal DNA (rDNA) and mitochondrial‐specific examination of methylation, targeted high‐resolution methylation analysis, and DNAge™ epigenetic aging clock analysis with a translatable model of voluntary murine endurance/resistance exercise training (progressive weighted wheel running, PoWeR), we provide evidence that exercise may mitigate epigenetic aging in skeletal muscle. Late‐life PoWeR from 22–24 months of age modestly but significantly attenuates an age‐associated shift toward promoter hypermethylation. The epigenetic age of muscle from old mice that PoWeR‐trained for eight weeks was approximately eight weeks younger than 24‐month‐old sedentary counterparts, which represents ~8% of the expected murine lifespan. These data provide a molecular basis for exercise as a therapy to attenuate skeletal muscle aging. [ABSTRACT FROM AUTHOR]

Published
2022
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11. Effects of Hepatitis C core protein on mitochondrial electron transport and production of reactive oxygen species

Author

Campbell, Roosevelt V., Yang, Yuanzheng, Wang, Ting, Rachamallu, Aparna, Li, Yanchun, Watowich, Stanley J., and Weinman, Steven A.

Subjects
Electron Transport, Base Sequence, Glutathione Disulfide, viruses, Viral Core Proteins, Calcium, Electrophoresis, Polyacrylamide Gel, Hepacivirus, Reactive Oxygen Species, Glutathione, Article, DNA Primers
Abstract

Viral infections frequently alter mitochondrial function with suppression or induction of apoptosis and enhanced generation of reactive oxygen species. The mechanisms of these effects are varied and mitochondria are affected by both direct interactions with viral proteins as well as by secondary effects of viral activated signaling cascades. This chapter describes methods used in our laboratory to assess the effects of the Hepatitis C virus core protein on mitochondrial ROS production, electron transport and Ca2+ uptake. These include measurements of the effects of in vitro incubation of liver mitochondria with purified core protein as well as assessment of the function of mitochondria in cells and tissues expressing core and other viral proteins. These methods are generally applicable to the study of viral-mitochondrial interactions.

Published
2009

12. An Assembly Model of Rift Valley Fever Virus

Author

Rusu, Mirabela, primary, Bonneau, Richard, additional, Holbrook, Michael R., additional, Watowich, Stanley J., additional, Birmanns, Stefan, additional, Wriggers, Willy, additional, and Freiberg, Alexander N., additional

Published
2012
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31. Biophysical characterization of hepatitis C virus core protein: implications for interactions within the virus and host

Author

Kunkel, Meghan and Watowich, Stanley J.

Subjects
*HEPATITIS C virus, *VIRAL genomes, *FLUORESCENCE spectroscopy, *PHYSIOLOGY
Abstract

A primary function of the hepatitis C virus (HCV) core protein is to package the viral genome within a nucleocapsid. In addition, core protein has been shown to interact with more than a dozen cellular proteins, and these interactions have been suggested to play critical roles in HCV pathogenesis. A more complete knowledge of the biophysical properties of the core protein may help to clarify its role in HCV pathogenesis and nucleocapsid assembly and provide a basis for the development of novel anti-HCV therapies. Here we report that recombinant mature core protein exists as a large multimer in solution under physiological conditions. Far-UV circular dichroism (CD) experiments showed that the mature core protein contains stable secondary structure. Studies with truncated core protein demonstrated that the C-terminal region of the core protein is critical for its folding and oligomerization. Intrinsic fluorescence spectroscopy and near-UV CD analysis indicated that the tryptophan-rich region (residues 76–113) is largely solvent-exposed and not likely responsible for multimerization of the mature core protein in vitro. [Copyright &y& Elsevier]

Published
2004
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