309 results on '"Wang, Lianguo"'
Search Results
2. Elastoplastic analysis on deformation and failure characteristics of surrounding rock of soft-coal roadway based on true triaxial loading and unloading tests
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Jiang, Chongyang, Wang, Lianguo, Guo, Jiaxing, and Wang, Shuai
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- 2024
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3. A whale optimization algorithm based on atom-like structure differential evolution for solving engineering design problems
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Tang, Junjie and Wang, Lianguo
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- 2024
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4. Whole-heart multiparametric optical imaging reveals sex-dependent heterogeneity in cAMP signaling and repolarization kinetics
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Caldwell, Jessica L, Lee, I-Ju, Ngo, Lena, Wang, Lianguo, Bahriz, Sherif, Xu, Bing, Bers, Donald M, Navedo, Manuel F, Bossuyt, Julie, Xiang, Yang K, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Cardiovascular ,Heart Disease ,2.1 Biological and endogenous factors ,Aetiology ,Mice ,Male ,Female ,Animals ,Cyclic AMP ,Kinetics ,Signal Transduction ,Myocytes ,Cardiac ,Optical Imaging - Abstract
Cyclic adenosine 3',5'-monophosphate (cAMP) is a key second messenger in cardiomyocytes responsible for transducing autonomic signals into downstream electrophysiological responses. Previous studies have shown intracellular heterogeneity and compartmentalization of cAMP signaling. However, whether cAMP signaling occurs heterogeneously throughout the intact heart and how this drives sex-dependent functional responses are unknown. Here, we developed and validated a novel cardiac-specific fluorescence resonance energy transfer-based cAMP reporter mouse and a combined voltage-cAMP whole-heart imaging system. We showed that in male hearts, cAMP was uniformly activated in response to pharmacological β-adrenergic stimulation. In contrast, female hearts showed that cAMP levels decayed faster in apical versus basal regions, which was associated with nonuniform action potential changes and notable changes in the direction of repolarization. Apical phosphodiesterase (PDE) activity was higher in female versus male hearts, and PDE inhibition prevented repolarization changes in female hearts. Thus, our imaging approach revealed sex-dependent regional breakdown of cAMP and associated electrophysiological differences.
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- 2023
5. Observations of Forbush Decreases of cosmic ray electrons and positrons with the Dark Matter Particle Explorer
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Alemanno, Francesca, An, Qi, Azzarello, Philipp, Barbato, Felicia Carla Tiziana, Bernardini, Paolo, Bi, XiaoJun, Cai, MingSheng, Casilli, Elisabetta, Catanzani, Enrico, Chang, Jin, Chen, DengYi, Chen, JunLing, Chen, ZhanFang, Cui, MingYang, Cui, TianShu, Cui, YuXing, Dai, HaoTing, De Benedittis, Antonio, De Mitri, Ivan, de Palma, Francesco, Deliyergiyev, Maksym, Di Santo, Margherita, Ding, Qi, Dong, TieKuang, Dong, ZhenXing, Donvito, Giacinto, Droz, David, Duan, JingLai, Duan, KaiKai, D'Urso, Domenico, Fan, RuiRui, Fan, YiZhong, Fang, Fang, Fang, Kun, Feng, ChangQing, Feng, Lei, Fusco, Piergiorgio, Gao, Min, Gargano, Fabio, Gong, Ke, Gong, YiZhong, Guo, DongYa, Guo, JianHua, Han, ShuangXue, Hu, YiMing, Huang, GuangShun, Huang, XiaoYuan, Huang, YongYi, Ionica, Maria, Jiang, Wei, Kong, Jie, Kotenko, Andrii, Kyratzis, Dimitrios, Li, ., Lei, ShiJun, Li, WenHao, Li, WeiLiang, Li, Xiang, Li, XianQiang, Liang, YaoMing, Liu, ChengMing, Liu, Hao, Liu, Jie, Liu, ShuBin, Liu, Yang, Loparco, Francesco, Luo, ChuanNing, Ma, Miao, Ma, PengXiong, Ma, Tao, Ma, XiaoYong, Marsella, Giovanni, Mazziotta, Mario Nicola, Mo, Dan, Niu, XiaoYang, Pan, Xu, Parenti, Andrea, Peng, WenXi, Peng, XiaoYan, Perrina, Chiara, Qiao, Rui, Rao, JiaNing, Ruina, Arshia, Salinas, MariaMunoz, Shangguan, Zhi, Shen, WeiHua, Shen, ZhaoQiang, Shen, ZhongTao, Silveri, Leandro, Song, JingXing, Stolpovskiy, Mikhail, Su, Hong, Su, Meng, Sun, HaoRan, Sun, ZhiYu, Surdo, Antonio, Teng, XueJian, Tykhonov, Andrii, Wang, JinZhou, Wang, LianGuo, Wang, Shen, Wang, ShuXin, Wang, XiaoLian, Wang, Ying, Wang, YanFang, Wang, YuanZhu, Wei, DaMing, Wei, JiaJu, Wei, YiFeng, Wu, Di, Wu, Jian, Wu, LiBo, Wu, Sha Sha, Wu, Xin, Xia, ZiQing, Xu, EnHeng, Xu, HaiTao, Xu, ZhiHui, Xu, ZunLei, Xue, GuoFeng, Xu, ZiZong, Yang, HaiBo, Yang, Peng, Yang, YaQing, Yao, Hui Jun, Yu, YuHong, Yuan, GuanWen, Yuan, Qiang, Yue, Chuan, Zang, JingJing, Zhang, ShengXia, Zhang, WenZhang, Zhang, Yan, Zhang, Yi, Zhang, YongJie, Zhang, YunLong, Zhang, YaPeng, Zhang, YongQiang, Zhang, ZhiYong, Zhang, Zhe, Zhao, Cong, Zhao, HongYun, Zhao, XunFeng, Zhou, ChangYi, Zhu, Yan, Chen, Wei, Feng, Li, Luo, Xi, and Zhu, ChengRui
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Astrophysics - High Energy Astrophysical Phenomena - Abstract
The Forbush Decrease (FD) represents the rapid decrease of the intensities of charged particles accompanied with the coronal mass ejections (CMEs) or high-speed streams from coronal holes. It has been mainly explored with ground-based neutron monitors network which indirectly measure the integrated intensities of all species of cosmic rays by counting secondary neutrons produced from interaction between atmosphere atoms and cosmic rays. The space-based experiments can resolve the species of particles but the energy ranges are limited by the relative small acceptances except for the most abundant particles like protons and helium. Therefore, the FD of cosmic ray electrons and positrons have just been investigated by the PAMELA experiment in the low energy range ($<5$ GeV) with limited statistics. In this paper, we study the FD event occurred in September, 2017, with the electron and positron data recorded by the Dark Matter Particle Explorer. The evolution of the FDs from 2 GeV to 20 GeV with a time resolution of 6 hours are given. We observe two solar energetic particle events in the time profile of the intensity of cosmic rays, the earlier and weak one has not been shown in the neutron monitor data. Furthermore, both the amplitude and recovery time of fluxes of electrons and positrons show clear energy-dependence, which is important in probing the disturbances of the interplanetary environment by the coronal mass ejections., Comment: This article is dedicated to the 72nd anniversary of People's Republic of China
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- 2021
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6. Study of the mechanical characteristics of coal-serial sandstone after high temperature treatment under true triaxial loading
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Wang, Shuai, Wang, Lianguo, Ren, Bo, Ding, Ke, Jiang, Chongyang, and Guo, Jiaxing
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- 2023
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7. Modelling phase equilibria in the CO2/CH4-H2O-NaCl system using the association equation of state
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Yang, Haolei, Bian, Xiaoqiang, and Wang, Lianguo
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- 2024
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8. Quantitative cross-species translators of cardiac myocyte electrophysiology: Model training, experimental validation, and applications
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Morotti, Stefano, Liu, Caroline, Hegyi, Bence, Ni, Haibo, Fogli Iseppe, Alex, Wang, Lianguo, Pritoni, Marco, Ripplinger, Crystal M, Bers, Donald M, Edwards, Andrew G, and Grandi, Eleonora
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Biological Sciences ,Medical Physiology ,Biomedical and Clinical Sciences ,Heart Disease ,Cardiovascular ,Development of treatments and therapeutic interventions ,5.1 Pharmaceuticals - Abstract
Animal experimentation is key in the evaluation of cardiac efficacy and safety of novel therapeutic compounds. However, interspecies differences in the mechanisms regulating excitation-contraction coupling can limit the translation of experimental findings from animal models to human physiology and undermine the assessment of drugs’ efficacy and safety. Here, we built a suite of translators for quantitatively mapping electrophysiological responses in ventricular myocytes across species. We trained these statistical operators using a broad dataset obtained by simulating populations of our biophysically detailed computational models of action potential and Ca2+ transient in mouse, rabbit, and human. We then tested our translators against experimental data describing the response to stimuli, such as ion channel block, change in beating rate, and β-adrenergic challenge. We demonstrate that this approach is well suited to predicting the effects of perturbations across different species or experimental conditions and suggest its integration into mechanistic studies and drug development pipelines.
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- 2021
9. Mechanical behavior and fracture characteristics of high-temperature sandstone under true triaxial loading conditions
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Wang, Shuai, Wang, Lianguo, Ren, Bo, Ding, Ke, Jiang, Chongyang, and Guo, Jiaxing
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- 2024
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10. Estimating CO2 solubility in ionic liquids by using machine learning methods
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Liu, Zongyang, Bian, Xiao-Qiang, Duan, Suling, Wang, Lianguo, and Fahim, Rayhanul Islam
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- 2023
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11. Role of Reduced Sarco-Endoplasmic Reticulum Ca2+-ATPase Function on Sarcoplasmic Reticulum Ca2+ Alternans in the Intact Rabbit Heart
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Wang, Lianguo, Myles, Rachel C, Lee, I-Ju, Bers, Donald M, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Cardiovascular ,Heart Disease ,sarco-endoplasmic reticulum Ca2+-ATPase ,sarcoplasmic reticulum Ca2+ ,optical mapping ,alternans ,arrhythmia ,Physiology ,Psychology ,Biochemistry and cell biology ,Medical physiology - Abstract
Sarcoplasmic reticulum (SR) Ca2+ cycling is tightly regulated by ryanodine receptor (RyR) Ca2+ release and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) Ca2+ uptake during each excitation-contraction coupling cycle. We previously showed that RyR refractoriness plays a key role in the onset of SR Ca2+ alternans in the intact rabbit heart, which contributes to arrhythmogenic action potential duration (APD) alternans. Recent studies have also implicated impaired SERCA function, a key feature of heart failure, in cardiac alternans and arrhythmias. However, the relationship between reduced SERCA function and SR Ca2+ alternans is not well understood. Simultaneous optical mapping of transmembrane potential (Vm) and SR Ca2+ was performed in isolated rabbit hearts (n = 10) using the voltage-sensitive dye RH237 and the low-affinity Ca2+ indicator Fluo-5N-AM. Alternans was induced by rapid ventricular pacing. SERCA was inhibited with cyclopiazonic acid (CPA; 1-10 μM). SERCA inhibition (1, 5, and 10 μM of CPA) resulted in dose-dependent slowing of SR Ca2+ reuptake, with the time constant (tau) increasing from 70.8 ± 3.5 ms at baseline to 85.5 ± 6.6, 129.9 ± 20.7, and 271.3 ± 37.6 ms, respectively (p < 0.05 vs. baseline for all doses). At fast pacing frequencies, CPA significantly increased the magnitude of SR Ca2+ and APD alternans, most strongly at 10 μM (pacing cycle length = 220 ms: SR Ca2+ alternans magnitude: 57.1 ± 4.7 vs. 13.4 ± 8.9 AU; APD alternans magnitude 3.8 ± 1.9 vs. 0.2 ± 0.19 AU; p < 0.05 10 μM of CPA vs. baseline for both). SERCA inhibition also promoted the emergence of spatially discordant alternans. Notably, at all CPA doses, alternation of SR Ca2+ release occurred prior to alternation of diastolic SR Ca2+ load as pacing frequency increased. Simultaneous optical mapping of SR Ca2+ and Vm in the intact rabbit heart revealed that SERCA inhibition exacerbates pacing-induced SR Ca2+ and APD alternans magnitude, particularly at fast pacing frequencies. Importantly, SR Ca2+ release alternans always occurred before the onset of SR Ca2+ load alternans. These findings suggest that even in settings of diminished SERCA function, relative refractoriness of RyR Ca2+ release governs the onset of intracellular Ca2+ alternans.
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- 2021
12. Adrenergic supersensitivity and impaired neural control of cardiac electrophysiology following regional cardiac sympathetic nerve loss.
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Tapa, Srinivas, Wang, Lianguo, Francis Stuart, Samantha D, Wang, Zhen, Jiang, Yanyan, Habecker, Beth A, and Ripplinger, Crystal M
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Myocardium ,Heart ,Sympathetic Nervous System ,Animals ,Mice ,Inbred C57BL ,Myocardial Infarction ,Calcium ,Isoproterenol ,Receptors ,Adrenergic ,beta ,Action Potentials ,Male ,Arrhythmias ,Cardiac ,Cardiac Electrophysiology ,Arrhythmias ,Cardiac ,Mice ,Inbred C57BL ,Receptors ,Adrenergic ,beta - Abstract
Myocardial infarction (MI) can result in sympathetic nerve loss in the infarct region. However, the contribution of hypo-innervation to electrophysiological remodeling, independent from MI-induced ischemia and fibrosis, has not been comprehensively investigated. We present a novel mouse model of regional cardiac sympathetic hypo-innervation utilizing a targeted-toxin (dopamine beta-hydroxylase antibody conjugated to saporin, DBH-Sap), and measure resulting electrophysiological and Ca2+ handling dynamics. Five days post-surgery, sympathetic nerve density was reduced in the anterior left ventricular epicardium of DBH-Sap hearts compared to control. In Langendorff-perfused hearts, there were no differences in mean action potential duration (APD80) between groups; however, isoproterenol (ISO) significantly shortened APD80 in DBH-Sap but not control hearts, resulting in a significant increase in APD80 dispersion in the DBH-Sap group. ISO also produced spontaneous diastolic Ca2+ elevation in DBH-Sap but not control hearts. In innervated hearts, sympathetic nerve stimulation (SNS) increased heart rate to a lesser degree in DBH-Sap hearts compared to control. Additionally, SNS produced APD80 prolongation in the apex of control but not DBH-Sap hearts. These results suggest that hypo-innervated hearts have regional super-sensitivity to circulating adrenergic stimulation (ISO), while having blunted responses to SNS, providing important insight into the mechanisms of arrhythmogenesis following sympathetic nerve loss.
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- 2020
13. Versatile hybrid nanoplatforms for treating periodontitis with chemical/photothermal therapy and reactive oxygen species scavenging
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Wang, Pei, Wang, Lianguo, Zhan, Yilan, Liu, Yifan, Chen, Ziqiang, Xu, Jia, Guo, Jun, Luo, Jun, Wei, Junchao, Tong, Fei, and Li, Zhihua
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- 2023
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14. Aging Disrupts Normal Time-of-Day Variation in Cardiac Electrophysiology
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Wang, Zhen, Tapa, Srinivas, Francis Stuart, Samantha D, Wang, Lianguo, Bossuyt, Julie, Delisle, Brian P, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Heart Disease ,Neurosciences ,Cardiovascular ,Action Potentials ,Adrenergic beta-Agonists ,Age Factors ,Aging ,Animals ,Arrhythmias ,Cardiac ,Calcium Signaling ,Cardiac Pacing ,Artificial ,Circadian Rhythm ,Gene Expression Regulation ,Heart Rate ,Isolated Heart Preparation ,Isoproterenol ,Kv1.5 Potassium Channel ,Male ,Mice ,Inbred C57BL ,Myocardium ,Receptors ,Adrenergic ,beta-1 ,Time Factors ,aging ,action potential ,cardiac arrhythmias ,circadian rhythm ,isoproterenol ,Cardiorespiratory Medicine and Haematology ,Clinical Sciences ,Cardiovascular System & Hematology ,Cardiovascular medicine and haematology ,Clinical sciences ,Medical physiology - Abstract
BackgroundCardiac gene expression and arrhythmia occurrence have time-of-day variation; however, daily changes in cardiac electrophysiology, arrhythmia susceptibility, and Ca2+ handling have not been characterized. Furthermore, how these patterns change with age is unknown.MethodsHearts were isolated during the light (zeitgeber time [ZT] 4 and ZT9) and dark cycle (ZT14 and ZT21) from adult (12-18 weeks) male mice. Hearts from aged (18-20 months) male mice were isolated at ZT4 and ZT14. All hearts were Langendorff-perfused for optical mapping with voltage- and Ca2+-sensitive dyes (n=4-7/group). Cardiac gene and protein expression were assessed with real-time polymerase chain reaction (n=4-6/group) and Western blot (n=3-4/group).ResultsAdult hearts had the shortest action potential duration (APD) and Ca2+ transient duration (CaTD) at ZT14 (APD80: ZT4: 45.4±4.1 ms; ZT9: 45.1±8.6 ms; ZT14: 34.7±4.2 ms; ZT21: 49.2±7.6 ms, P
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- 2020
15. Extreme Individual Guided Artificial Bee Colony Algorithm
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CHEN Lan, WANG Lianguo
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artificial bee colony (abc) algorithm ,extreme individual guidance ,small probability mutation ,ob-jective function value ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
To overcome the drawbacks of poor development ability, easy to fall into local optimum, slow conver-gence speed of artificial bee colony (ABC) algorithm in solving function optimization problems, an extreme indi-vidual guided artificial bee colony (EABC) algorithm is proposed. Firstly, global extremum and neighborhood ex-tremum individuals are used to guide search for employed bees and following bees. The global extremum individual guided search is good for the retention and development of excellent individuals in the population, so that the algorithm jumps out of local extremum and avoids premature convergence. The neighborhood extremum individual guided search is good for enhancing the search accuracy and improving the convergence speed of the algorithm, and the random number ris used to balance two search mechanisms. Secondly, the small probability mutation operator is introduced into search process, and each dimension of bee individual is mutated with a small probability to overcome local extremum and premature convergence of the algorithm. Finally, the greedy selection strategy based on the value of objective function is adopted to improve the optimization performance of the algorithm. Simulation experiments are carried out with 28 test functions and the algorithm proposed in this paper is compared with other algorithms. Experimental results show that the improved algorithm has higher optimization performance and faster convergence speed.
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- 2022
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16. A shuffled frog leaping algorithm with contraction factor and its application in mechanical optimum design
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Wang, Lianguo and Liu, Xiaojuan
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- 2022
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17. Different paths, same destination: divergent action potential responses produce conserved cardiac fight‐or‐flight response in mouse and rabbit hearts
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Wang, Lianguo, Morotti, Stefano, Tapa, Srinivas, Stuart, Samantha D Francis, Jiang, Yanyan, Wang, Zhen, Myles, Rachel C, Brack, Kieran E, Ng, G André, Bers, Donald M, Grandi, Eleonora, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Heart Disease ,Neurosciences ,Cardiovascular ,Action Potentials ,Animals ,Calcium Signaling ,Heart ,Heart Rate ,Male ,Mice ,Mice ,Inbred C57BL ,Models ,Cardiovascular ,Myocardial Contraction ,Rabbits ,Stress ,Physiological ,Sympathetic Nervous System ,Optical mapping ,sympathetic activation ,intracellular Ca2+ ,action potential ,mathematical modelling ,Biological Sciences ,Medical and Health Sciences ,Physiology ,Biological sciences ,Biomedical and clinical sciences ,Health sciences - Abstract
Key pointsCardiac electrophysiology and Ca2+ handling change rapidly during the fight-or-flight response to meet physiological demands. Despite dramatic differences in cardiac electrophysiology, the cardiac fight-or-flight response is highly conserved across species. In this study, we performed physiological sympathetic nerve stimulation (SNS) while optically mapping cardiac action potentials and intracellular Ca2+ transients in innervated mouse and rabbit hearts. Despite similar heart rate and Ca2+ handling responses between mouse and rabbit hearts, we found notable species differences in spatio-temporal repolarization dynamics during SNS. Species-specific computational models revealed that these electrophysiological differences allowed for enhanced Ca2+ handling (i.e. enhanced inotropy) in each species, suggesting that electrophysiological responses are fine-tuned across species to produce optimal cardiac fight-or-flight responses.AbstractSympathetic activation of the heart results in positive chronotropy and inotropy, which together rapidly increase cardiac output. The precise mechanisms that produce the electrophysiological and Ca2+ handling changes underlying chronotropic and inotropic responses have been studied in detail in isolated cardiac myocytes. However, few studies have examined the dynamic effects of physiological sympathetic nerve activation on cardiac action potentials (APs) and intracellular Ca2+ transients (CaTs) in the intact heart. Here, we performed bilateral sympathetic nerve stimulation (SNS) in fully innervated, Langendorff-perfused rabbit and mouse hearts. Dual optical mapping with voltage- and Ca2+ -sensitive dyes allowed for analysis of spatio-temporal AP and CaT dynamics. The rabbit heart responded to SNS with a monotonic increase in heart rate (HR), monotonic decreases in AP and CaT duration (APD, CaTD), and a monotonic increase in CaT amplitude. The mouse heart had similar HR and CaT responses; however, a pronounced biphasic APD response occurred, with initial prolongation (50.9 ± 5.1 ms at t = 0 s vs. 60.6 ± 4.1 ms at t = 15 s, P
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- 2019
18. Exposure to Secondhand Smoke and Arrhythmogenic Cardiac Alternans in a Mouse Model
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Wang, Zhen, Wang, Lianguo, Tapa, Srinivas, Pinkerton, Kent E, Chen, Chao-Yin, and Ripplinger, Crystal M
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Biomedical and Clinical Sciences ,Clinical Sciences ,Cardiovascular ,Tobacco ,Health Effects of Indoor Air Pollution ,Heart Disease ,Tobacco Smoke and Health ,Action Potentials ,Animals ,Arrhythmias ,Cardiac ,Calcium ,Carbon Monoxide ,Cardiac Pacing ,Artificial ,Electrocardiography ,Heart ,Male ,Mice ,Inbred C57BL ,Nicotine ,Particulate Matter ,Perfusion ,Tobacco Smoke Pollution ,Ventricular Fibrillation ,Voltage-Sensitive Dye Imaging ,Environmental Sciences ,Medical and Health Sciences ,Toxicology ,Biomedical and clinical sciences ,Environmental sciences ,Health sciences - Abstract
BackgroundEpidemiological evidence suggests that a majority of deaths attributed to secondhand smoke (SHS) exposure are cardiovascular related. However, to our knowledge, the impact of SHS on cardiac electrophysiology, [Formula: see text] handling, and arrhythmia risk has not been studied.ObjectivesThe purpose of this study was to investigate the impact of an environmentally relevant concentration of SHS on cardiac electrophysiology and indicators of arrhythmia.MethodsMale C57BL/6 mice were exposed to SHS [total suspended particles (THS): [Formula: see text], nicotine: [Formula: see text], carbon monoxide: [Formula: see text], or filtered air (FA) for 4, 8, or 12 wk ([Formula: see text]]. Hearts were excised and Langendorff perfused for dual optical mapping with voltage- and [Formula: see text]-sensitive dyes.ResultsAt slow pacing rates, SHS exposure did not alter baseline electrophysiological parameters. With increasing pacing frequency, action potential duration (APD), and intracellular [Formula: see text] alternans magnitude progressively increased in all groups. At 4 and 8 wk, there were no statistical differences in APD or [Formula: see text] alternans magnitude between SHS and FA groups. At 12 wk, both APD and [Formula: see text] alternans magnitude were significantly increased in the SHS compared to FA group ([Formula: see text]). SHS exposure did not impact the time constant of [Formula: see text] transient decay ([Formula: see text]) at any exposure time point. At 12 wk exposure, the recovery of [Formula: see text] transient amplitude with premature stimuli was slightly (but nonsignificantly) delayed in SHS compared to FA hearts, suggesting that [Formula: see text] release via ryanodine receptors may be impaired.ConclusionsIn male mice, chronic exposure to SHS at levels relevant to social situations in humans increased their susceptibility to cardiac alternans, a known precursor to ventricular arrhythmia. https://doi.org/10.1289/EHP3664.
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- 2018
19. Antiarrhythmic effects of interleukin 1 inhibition after myocardial infarction
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De Jesus, Nicole M, Wang, Lianguo, Lai, Johnny, Rigor, Robert R, Stuart, Samantha D Francis, Bers, Donald M, Lindsey, Merry L, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Cardiovascular ,Heart Disease - Coronary Heart Disease ,Heart Disease ,Animals ,Anti-Arrhythmia Agents ,Arrhythmias ,Cardiac ,Disease Models ,Animal ,Excitation Contraction Coupling ,Interleukin 1 Receptor Antagonist Protein ,Interleukin-1beta ,Mice ,Myocardial Infarction ,Antiarrhythmic agents ,Action potentials ,Calcium ,Interleukins ,Myocardial infarction ,Biomedical Engineering ,Cardiorespiratory Medicine and Haematology ,Cardiovascular System & Hematology ,Cardiovascular medicine and haematology - Abstract
BackgroundInterleukin 1β (IL-1β) is a key regulator of the inflammatory response after myocardial infarction (MI) by modulating immune cell recruitment, cytokine production, and extracellular matrix turnover. Elevated levels of IL-1β are associated with adverse remodeling, and inhibition of IL-1 signaling after MI results in improved contractile function.ObjectiveThe goal of this study was to determine whether IL-1 signaling also contributes to post-MI arrhythmogenesis.MethodsMI was created in 2 murine models of elevated inflammation: atherosclerotic on the Western diet or wild-type with a subseptic dose of lipopolysaccharide. The role of IL-1β was assessed with the IL-1 receptor antagonist anakinra (10 mg/(kg·d), starting 24 hours post-MI).ResultsIn vivo and ex vivo molecular imaging showed reduced myocardial inflammation after a 4-day course of anakinra treatment, despite no change in infarct size. At day 5 post-MI, high-speed optical mapping of transmembrane potential and intracellular Ca2+ in isolated hearts revealed that IL-1β inhibition improved conduction velocity, reduced action potential duration dispersion, improved intracellular Ca2+ handling, decreased transmembrane potential and Ca2+ alternans magnitude, and reduced spontaneous and inducible ventricular arrhythmias. These functional improvements were linked to increased expression of connexin 43 and sarcoplasmic reticulum Ca2+-ATPase.ConclusionThis study revealed a novel mechanism for IL-1β in contributing to defective excitation-contraction coupling and arrhythmogenesis in the post-MI heart. Our results suggest that inhibition of IL-1 signaling post-MI may represent a novel antiarrhythmic therapy.
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- 2017
20. β-Adrenergic Inhibition Prevents Action Potential and Calcium Handling Changes during Regional Myocardial Ischemia
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Murphy, Shannon R, Wang, Lianguo, Wang, Zhen, Domondon, Philip, Lang, Di, Habecker, Beth A, Myles, Rachel C, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Heart Disease - Coronary Heart Disease ,Prevention ,Cardiovascular ,Heart Disease ,ischemia ,arrhythmia ,beta blocker ,sarcoplasmic reticulum ,calcium ,Physiology ,Psychology ,Biochemistry and cell biology ,Medical physiology - Abstract
β-adrenergic receptor (β-AR) blockers may be administered during acute myocardial infarction (MI), as they reduce energy demand through negative chronotropic and inotropic effects and prevent ischemia-induced arrhythmogenesis. However, the direct effects of β-AR blockers on ventricular electrophysiology and intracellular Ca2+ handling during ischemia remain unknown. Using optical mapping of transmembrane potential (with RH237) and sarcoplasmic reticulum (SR) Ca2+ (with the low-affinity indicator Fluo-5N AM), the effects of 15 min of regional ischemia were assessed in isolated rabbit hearts (n = 19). The impact of β-AR inhibition on isolated hearts was assessed by pre-treatment with 100 nM propranolol (Prop) prior to ischemia (n = 7). To control for chronotropy and inotropy, hearts were continuously paced at 3.3 Hz and contraction was inhibited with 20 μM blebbistatin. Untreated ischemic hearts displayed prototypical shortening of action potential duration (APD80) in the ischemic zone (IZ) compared to the non-ischemic zone (NI) at 10 and 15 min ischemia, whereas APD shortening was prevented with Prop. Untreated ischemic hearts also displayed significant changes in SR Ca2+ handling in the IZ, including prolongation of SR Ca2+ reuptake and SR Ca2+ alternans, which were prevented with Prop pre-treatment. At 5 min ischemia, Prop pre-treated hearts also showed larger SR Ca2+ release amplitude in the IZ compared to untreated hearts. These results suggest that even when controlling for chronotropic and inotropic effects, β-AR inhibition has a favorable effect during acute regional ischemia via direct effects on APD and Ca2+ handling.
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- 2017
21. Scientific objectives and payloads of Tianwen-1, China’s first Mars exploration mission
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Zou, Yongliao, Zhu, Yan, Bai, Yunfei, Wang, Lianguo, Jia, Yingzhuo, Shen, Weihua, Fan, Yu, Liu, Yang, Wang, Chi, Zhang, Aibing, Yu, Guobin, Dong, Jihong, Shu, Rong, He, Zhiping, Zhang, Tielong, Du, Aimin, Fan, Mingyi, Yang, Jianfeng, Zhou, Bin, Wang, Yi, and Peng, Yongqing
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- 2021
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22. Molecular Mechanisms and New Treatment Paradigm for Atrial Fibrillation
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Sirish, Padmini, Li, Ning, Timofeyev, Valeriy, Zhang, Xiao-Dong, Wang, Lianguo, Yang, Jun, Lee, Kin Sing Stephen, Bettaieb, Ahmed, Ma, Sin Mei, Lee, Jeong Han, Su, Demetria, Lau, Victor C, Myers, Richard E, Lieu, Deborah K, López, Javier E, Young, J Nilas, Yamoah, Ebenezer N, Haj, Fawaz, Ripplinger, Crystal M, Hammock, Bruce D, and Chiamvimonvat, Nipavan
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Biomedical and Clinical Sciences ,Medical Physiology ,Cardiovascular Medicine and Haematology ,Clinical Sciences ,Heart Disease ,Cardiovascular ,2.1 Biological and endogenous factors ,5.1 Pharmaceuticals ,Development of treatments and therapeutic interventions ,Aetiology ,Animals ,Anti-Arrhythmia Agents ,Anti-Inflammatory Agents ,Atrial Fibrillation ,Atrial Remodeling ,Disease Models ,Animal ,Enzyme Inhibitors ,Heart Atria ,Male ,Mice ,Mice ,Inbred C57BL ,Oxidative Stress ,animal model ,arrhythmia ,atrial fibrillation ,eicosanoids ,inflammation ,Cardiorespiratory Medicine and Haematology ,Cardiovascular System & Hematology ,Cardiovascular medicine and haematology ,Clinical sciences ,Medical physiology - Abstract
BackgroundAtrial fibrillation represents the most common arrhythmia leading to increased morbidity and mortality, yet, current treatment strategies have proven inadequate. Conventional treatment with antiarrhythmic drugs carries a high risk for proarrhythmias. The soluble epoxide hydrolase enzyme catalyzes the hydrolysis of anti-inflammatory epoxy fatty acids, including epoxyeicosatrienoic acids from arachidonic acid to the corresponding proinflammatory diols. Therefore, the goal of the study is to directly test the hypotheses that inhibition of the soluble epoxide hydrolase enzyme can result in an increase in the levels of epoxyeicosatrienoic acids, leading to the attenuation of atrial structural and electric remodeling and the prevention of atrial fibrillation.Methods and resultsFor the first time, we report findings that inhibition of soluble epoxide hydrolase reduces inflammation, oxidative stress, atrial structural, and electric remodeling. Treatment with soluble epoxide hydrolase inhibitor significantly reduces the activation of key inflammatory signaling molecules, including the transcription factor nuclear factor κ-light-chain-enhancer, mitogen-activated protein kinase, and transforming growth factor-β.ConclusionsThis study provides insights into the underlying molecular mechanisms leading to atrial fibrillation by inflammation and represents a paradigm shift from conventional antiarrhythmic drugs, which block downstream events to a novel upstream therapeutic target by counteracting the inflammatory processes in atrial fibrillation.
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- 2016
23. Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130
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Olivas, Antoinette, Gardner, Ryan T, Wang, Lianguo, Ripplinger, Crystal M, Woodward, William R, and Habecker, Beth A
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Biomedical and Clinical Sciences ,Neurosciences ,Cardiovascular ,Heart Disease ,Heart Disease - Coronary Heart Disease ,Aetiology ,2.1 Biological and endogenous factors ,Acetylcholine ,Animals ,Cell Transdifferentiation ,Choline O-Acetyltransferase ,Cytokine Receptor gp130 ,Disease Models ,Animal ,Dopamine beta-Hydroxylase ,Female ,Ganglia ,Sympathetic ,Genotype ,Male ,Membrane Potentials ,Mice ,Mice ,Inbred C57BL ,Mice ,Transgenic ,Myocardial Reperfusion Injury ,Neurons ,Norepinephrine ,Rabbits ,Tyrosine 3-Monooxygenase ,Vesicular Acetylcholine Transport Proteins ,cholinergic ,cytokine ,myocardial infarction ,sympathetic ,transdifferentiation ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Sympathetic and parasympathetic control of the heart is a classic example of norepinephrine (NE) and acetylcholine (ACh) triggering opposing actions. Sympathetic NE increases heart rate and contractility through activation of β receptors, whereas parasympathetic ACh slows the heart through muscarinic receptors. Sympathetic neurons can undergo a developmental transition from production of NE to ACh and we provide evidence that mouse cardiac sympathetic nerves transiently produce ACh after myocardial infarction (MI). ACh levels increased in viable heart tissue 10-14 d after MI, returning to control levels at 21 d, whereas NE levels were stable. At the same time, the genes required for ACh synthesis increased in stellate ganglia, which contain most of the sympathetic neurons projecting to the heart. Immunohistochemistry 14 d after MI revealed choline acetyltransferase (ChAT) in stellate sympathetic neurons and vesicular ACh transporter immunoreactivity in tyrosine hydroxylase-positive cardiac sympathetic fibers. Finally, selective deletion of the ChAT gene from adult sympathetic neurons prevented the infarction-induced increase in cardiac ACh. Deletion of the gp130 cytokine receptor from sympathetic neurons prevented the induction of cholinergic genes after MI, suggesting that inflammatory cytokines induce the transient acquisition of a cholinergic phenotype in cardiac sympathetic neurons. Ex vivo experiments examining the effect of NE and ACh on rabbit cardiac action potential duration revealed that ACh blunted both the NE-stimulated decrease in cardiac action potential duration and increase in myocyte calcium transients. This raises the possibility that sympathetic co-release of ACh and NE may impair adaptation to high heart rates and increase arrhythmia susceptibility.Significance statementSympathetic neurons normally make norepinephrine (NE), which increases heart rate and the contractility of cardiac myocytes. We found that, after myocardial infarction, the sympathetic neurons innervating the heart begin to make acetylcholine (ACh), which slows heart rate and decreases contractility. Several lines of evidence confirmed that the source of ACh was sympathetic nerves rather than parasympathetic nerves that are the normal source of ACh in the heart. Global application of NE with or without ACh to ex vivo hearts showed that ACh partially reversed the NE-stimulated decrease in cardiac action potential duration and increase in myocyte calcium transients. That suggests that sympathetic co-release of ACh and NE may impair adaptation to high heart rates and increase arrhythmia susceptibility.
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- 2016
24. Performance Evaluation Method for Intelligent Computing Components for Space Applications
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Xie, Man, primary, Wang, Lianguo, additional, Ma, Miao, additional, and Zhang, Pengfei, additional
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- 2023
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25. Research on an Intelligent Mining Complete System of a Fully Mechanized Mining Face in Thin Coal Seam
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Ren, Bo, primary, Ding, Ke, additional, Wang, Lianguo, additional, Wang, Shuai, additional, Jiang, Chongyang, additional, and Guo, Jiaxing, additional
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- 2023
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26. Decreased inward rectifying K+ current and increased ryanodine receptor sensitivity synergistically contribute to sustained focal arrhythmia in the intact rabbit heart
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Myles, Rachel C, Wang, Lianguo, Bers, Donald M, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Heart Disease ,Cardiovascular ,Action Potentials ,Animals ,Arrhythmias ,Cardiac ,Caffeine ,Calcium Channel Blockers ,Calcium Signaling ,Heart ,Male ,Myocardium ,Norepinephrine ,Potassium Channel Blockers ,Potassium Channels ,Inwardly Rectifying ,Rabbits ,Ryanodine Receptor Calcium Release Channel ,Biological Sciences ,Medical and Health Sciences ,Physiology ,Biological sciences ,Biomedical and clinical sciences ,Health sciences - Abstract
Key pointsHeart failure leads to dramatic electrophysiological remodelling as a result of numerous cellular and tissue-level changes. Important cellular changes include increased sensitivity of ryanodine receptors (RyRs) to Ca(2+) release and down-regulation of the inward rectifying K(+) current (IK1), both of which contribute to triggered action potentials in isolated cells. We studied the role of increased RyR sensitivity and decreased IK1 in contributing to focal arrhythmia in the intact non-failing rabbit heart using optical mapping and pharmacological manipulation of RyRs and IK1. Neither increased RyR sensitivity or decreased IK1 alone led to significant increases in arrhythmia following local sympathetic stimulation; however, in combination, these two factors led to a significant increase in premature ventricular complexes and focal ventricular tachycardia. These results suggest synergism between increased RyR sensitivity and decreased IK1 in contributing to focal arrhythmia in the intact heart and may provide important insights into novel anti-arrhythmic treatments in heart failure.AbstractHeart failure (HF) results in dramatic electrophysiological remodelling, including increased sensitivity of ryanodine receptors (RyRs) and decreased inward rectifying K(+) current (IK1), which predisposes HF myocytes to delayed afterdepolarizations and triggered activity. Therefore, we sought to determine the role of increased RyR sensitivity and decreased IK1 in contributing to focal arrhythmia in the intact non-failing heart. Optical mapping of transmembrane potential and intracellular Ca(2+) was performed in Langendorff-perfused rabbit hearts (n = 15). Local β-adrenergic receptor stimulation with noradrenaline (norepinephrine; NA, 50 μl, 250 μM) was applied to elicit focal activity (premature ventricular complexes (PVCs) or ventricular tachycardia (VT ≥ 3 beats)). NA was administered under control conditions (CTL) and following pretreatment with 50 μM BaCl2 to reduce IK1, or 200 μM caffeine (Caff) to sensitize RyRs, both alone and in combination. Local NA injection resulted in Ca(2+)-driven PVCs arising from the injection site in all hearts studied. No increase in NA-mediated PVCs was observed following pretreatment with either BaCl2 or Caff alone (CTL: 1.1 ± 0.7, BaCl2: 1.0 ± 0.7, Caff: 1.3 ± 0.8 PVCs/injection, P not significant). However, pretreatment with the combination of BaCl2 + Caff resulted in a significant increase in PVCs (2.3 ± 2.8 PVCs/injection, P < 0.05 vs. CTL, BaCl2, Caff). Additionally, pretreatment with BaCl2 + Caff led to sustained monomorphic VT arising from the NA application site in all hearts studied, which lasted up to 6 min following a single NA injection. VT was never observed under any other condition suggesting synergism between increased RyR sensitivity and decreased IK1 in contributing to focal activity. These findings may have important implications for the understanding and prevention of focal arrhythmia in HF.
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- 2015
27. Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart.
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Wang, Lianguo, De Jesus, Nicole M, and Ripplinger, Crystal M
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Biochemistry and Cell Biology ,Biological Sciences ,Cardiovascular ,Heart Disease ,Animals ,Arrhythmias ,Cardiac ,Calcium ,Cytosol ,Fluorescent Dyes ,Heart ,Isolated Heart Preparation ,Membrane Potentials ,Myocardium ,Rabbits ,Sarcoplasmic Reticulum ,Voltage-Sensitive Dye Imaging ,Molecular Biology ,Issue 103 ,optical mapping ,sarcoplasmic reticulum ,calcium ,calcium-sensitive dye ,voltage-sensitive dye ,arrhythmia ,cardiac electrophysiology ,excitation-contraction coupling ,rabbit ,Psychology ,Cognitive Sciences ,Biochemistry and cell biology - Abstract
Sarcoplasmic reticulum (SR) Ca2+ handling plays a key role in normal excitation-contraction coupling and aberrant SR Ca2+ handling is known to play a significant role in certain types of arrhythmia. Because arrhythmias are spatially distinct, emergent phenomena, they must be investigated at the tissue level. However, methods for directly probing SR Ca2+ in the intact heart remain limited. This article describes the protocol for dual optical mapping of transmembrane potential (Vm) and free intra-SR [Ca2+] ([Ca2+]SR) in the Langendorff-perfused rabbit heart. This approach takes advantage of the low-affinity Ca2+ indicator Fluo-5N, which has minimal fluorescence in the cytosol where intracellular [Ca2+] ([Ca2+]i) is relatively low but exhibits significant fluorescence in the SR lumen where [Ca2+]SR is in the millimolar range. In addition to revealing SR Ca2+ characteristics spatially across the epicardial surface of the heart, this approach has the distinct advantage of simultaneous monitoring of Vm, allowing for investigations into the bidirectional relationship between Vm and SR Ca2+ and the role of SR Ca2+ in arrhythmogenic phenomena.
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- 2015
28. Atherosclerosis exacerbates arrhythmia following myocardial infarction: Role of myocardial inflammation.
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De Jesus, Nicole M, Wang, Lianguo, Herren, Anthony W, Wang, Jingjing, Shenasa, Fatemah, Bers, Donald M, Lindsey, Merry L, and Ripplinger, Crystal M
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Animals ,Mice ,Inbred C57BL ,Mice ,Myocarditis ,Myocardial Infarction ,Disease Models ,Animal ,Electrocardiography ,Male ,Atherosclerosis ,Arrhythmias ,Cardiac ,Arrhythmia ,Inflammation ,Myocardial infarction ,Optical mapping ,Inbred C57BL ,Disease Models ,Animal ,Arrhythmias ,Cardiac ,Cardiovascular System & Hematology ,Cardiorespiratory Medicine and Haematology ,Biomedical Engineering - Abstract
BackgroundAtherosclerotic animal models show increased recruitment of inflammatory cells to the heart after myocardial infarction (MI), which impacts ventricular function and remodeling.ObjectiveThe purpose of this study was to determine whether increased myocardial inflammation after MI also contributes to arrhythmias.MethodsMI was created in 3 mouse models: (1) atherosclerotic (apolipoprotein E deficient [ApoE(-/-)] on atherogenic diet, n = 12); (2) acute inflammation (wild-type [WT] given daily lipopolysaccharide [LPS] 10 μg/day, n = 7); and (3) WT (n = 14). Sham-operated (n = 4) mice also were studied. Four days post-MI, an inflammatory protease-activatable fluorescent probe (Prosense680) was injected intravenously to quantify myocardial inflammation on day 5. Optical mapping with voltage-sensitive dye was performed on day 5 to assess electrophysiology and arrhythmia susceptibility.ResultsInflammatory activity (Prosense680 fluorescence) was increased approximately 2-fold in ApoE+MI and LPS+MI hearts vs WT+MI (P
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- 2015
29. In-situ microscale visualization experiments on microcracking and microdeformation behaviour around a pre-crack tip in a three-point bending sandstone
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Lu, Yinlong, Li, Wenshuai, Wang, Lianguo, Meng, Xingyu, Wang, Bin, Zhang, Kaiwen, and Zhang, Xiaodong
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- 2019
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30. Optical Mapping of Sarcoplasmic Reticulum Ca2+ in the Intact Heart
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Wang, Lianguo, Myles, Rachel C, De Jesus, Nicole M, Ohlendorf, Alex KP, Bers, Donald M, and Ripplinger, Crystal M
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Medical Physiology ,Biomedical and Clinical Sciences ,Cardiovascular ,Heart Disease ,2.1 Biological and endogenous factors ,Aetiology ,Action Potentials ,Adrenergic beta-Agonists ,Animals ,Caffeine ,Calcium ,Calcium Signaling ,Cardiac Pacing ,Artificial ,Excitation Contraction Coupling ,In Vitro Techniques ,Isoproterenol ,Myocytes ,Cardiac ,Perfusion ,Rabbits ,Receptors ,Adrenergic ,beta ,Refractory Period ,Electrophysiological ,Ryanodine Receptor Calcium Release Channel ,Sarcoplasmic Reticulum ,Sarcoplasmic Reticulum Calcium-Transporting ATPases ,Time Factors ,Ventricular Fibrillation ,Voltage-Sensitive Dye Imaging ,arrhythmias ,cardiac ,ventricular fibrillation ,arrhythmias ,cardiac ,Cardiorespiratory Medicine and Haematology ,Clinical Sciences ,Cardiovascular System & Hematology ,Cardiovascular medicine and haematology ,Clinical sciences - Abstract
RationaleSarcoplasmic reticulum (SR) Ca(2+) cycling is key to normal excitation-contraction coupling but may also contribute to pathological cardiac alternans and arrhythmia.ObjectiveTo measure intra-SR free [Ca(2+)] ([Ca(2+)]SR) changes in intact hearts during alternans and ventricular fibrillation (VF).Methods and resultsSimultaneous optical mapping of Vm (with RH237) and [Ca(2+)]SR (with Fluo-5N AM) was performed in Langendorff-perfused rabbit hearts. Alternans and VF were induced by rapid pacing. SR Ca(2+) and action potential duration (APD) alternans occurred in-phase, but SR Ca(2+) alternans emerged first as cycle length was progressively reduced (217±10 versus 190±13 ms; P
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- 2014
31. The Effect of Different Factors on Poly(lactic-co-glycolic acid) Nanoparticle Properties and Drug Release Behaviors When Co-Loaded with Hydrophilic and Hydrophobic Drugs.
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Wang, Lianguo, Wang, Pei, Liu, Yifan, Mustafa Mahayyudin, Muhammad Atae, Li, Rong, Zhang, Weilun, Zhan, Yilan, and Li, Zhihua
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NANOPARTICLES , *GLYCOLIC acid , *ORGANIC solvents , *PROPYLENE carbonate , *ETHYL acetate , *SERUM albumin , *DOXORUBICIN , *IMMUNE response , *DICHLOROMETHANE - Abstract
Poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) are versatile drug nanocarriers with a wide spectrum of applications owing to their extensive advantages, including biodegradability, non-toxic side effects, and low immunogenicity. Among the numerous nanoparticle preparation methods available for PLGA NPs (the hydrophobic polymer), one of the most extensively utilized preparations is the sonicated-emulsified solvent evaporation method, owing to its simplicity, speed, convenience, and cost-effectiveness. Nevertheless, several factors can influence the outcomes, such as the types of concentration of the surfactants and organic solvents, as well as the volume of the aqueous phase. The objective of this article is to explore the influence of these factors on the properties of PLGA NPs and their drug release behavior following encapsulation. Herein, PLGA NPs were fabricated using bovine serum albumin (BSA) as a surfactant to investigate the impact of influencing factors, including different water-soluble organic solvents such as propylene carbonate (PC), ethyl acetate (PA), and dichloromethane (DCM). Notably, the size of PLGA NPs was smaller in the EA group compared to that in the DCM group. Moreover, PLGA NPs showed excellent stability, ascribed to the presence of the BSA surfactant. Furthermore, PLGA NPs were co-loaded with varying concentrations of hydrophilic drugs (doxorubicin hydrochloride) and hydrophobic drugs (celecoxib), and exhibited pH-sensitive drug release behavior in PBS with pH 7.4 and pH 5.5. [ABSTRACT FROM AUTHOR]
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- 2024
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32. Experimental Study on the Relationship between the Degree of Surrounding Rock Fragmentation and the Adaptability of Anchor Support
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Wang, Shuai, primary, Wang, Lianguo, additional, Tang, Furong, additional, Ding, Ke, additional, Li, Zhaolin, additional, Ren, Bo, additional, Jiang, Chongyang, additional, and Guo, Jiaxing, additional
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- 2023
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33. Classification-design-optimization integrated picking robots: a review
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Xiang, Jingyang, primary, Wang, Lianguo, additional, Li, Li, additional, Lai, Kee-Hung, additional, and Cai, Wei, additional
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- 2023
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34. Diabetic hyperglycaemia activates CaMKII and arrhythmias by O-linked glycosylation
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Erickson, Jeffrey R, Pereira, Laetitia, Wang, Lianguo, Han, Guanghui, Ferguson, Amanda, Dao, Khanha, Copeland, Ronald J, Despa, Florin, Hart, Gerald W, Ripplinger, Crystal M, and Bers, Donald M
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Medical Physiology ,Biomedical and Clinical Sciences ,Cardiovascular ,Diabetes ,Heart Disease ,Metabolic and endocrine ,Acetylglucosamine ,Animals ,Arrhythmias ,Cardiac ,Benzylamines ,Brain ,Calcium ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,Diabetes Complications ,Diazooxonorleucine ,Enzyme Activation ,Glucose ,Glycosylation ,Humans ,Hyperglycemia ,Mice ,Myocardium ,Myocytes ,Cardiac ,Rats ,Sarcoplasmic Reticulum ,Sulfonamides ,General Science & Technology - Abstract
Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is an enzyme with important regulatory functions in the heart and brain, and its chronic activation can be pathological. CaMKII activation is seen in heart failure, and can directly induce pathological changes in ion channels, Ca(2+) handling and gene transcription. Here, in human, rat and mouse, we identify a novel mechanism linking CaMKII and hyperglycaemic signalling in diabetes mellitus, which is a key risk factor for heart and neurodegenerative diseases. Acute hyperglycaemia causes covalent modification of CaMKII by O-linked N-acetylglucosamine (O-GlcNAc). O-GlcNAc modification of CaMKII at Ser 279 activates CaMKII autonomously, creating molecular memory even after Ca(2+) concentration declines. O-GlcNAc-modified CaMKII is increased in the heart and brain of diabetic humans and rats. In cardiomyocytes, increased glucose concentration significantly enhances CaMKII-dependent activation of spontaneous sarcoplasmic reticulum Ca(2+) release events that can contribute to cardiac mechanical dysfunction and arrhythmias. These effects were prevented by pharmacological inhibition of O-GlcNAc signalling or genetic ablation of CaMKIIδ. In intact perfused hearts, arrhythmias were aggravated by increased glucose concentration through O-GlcNAc- and CaMKII-dependent pathways. In diabetic animals, acute blockade of O-GlcNAc inhibited arrhythmogenesis. Thus, O-GlcNAc modification of CaMKII is a novel signalling event in pathways that may contribute critically to cardiac and neuronal pathophysiology in diabetes and other diseases.
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- 2013
35. Experimental Study on Effect of Nano-SiO2 on Shear Strength and Rheological Properties of Cement
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JIANG Chongyang, WANG Lianguo, LI Wenshuai, WANG Bin
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cement ,direct shear test ,shear strength ,nano-sio2 ,water reducer ,rheological property ,grouting ,Mining engineering. Metallurgy ,TN1-997 - Abstract
In order to study the effect of nano-SiO2 on shear strength and rheological properties of cement grout, a series of direct shear tests were conducted under multiple constant normal loads for cement grouted samples with water-cement ratio of 1.0 and 3-day curing time. Then, the influence of nano-SiO2 on shear behavior and shear strength parameters(τp, c and φ) of samples was studied, and the rheological properties of grouts after adding super plasticizer were further studied. The results showed that with the increase of shear displacement, the shear stress increased gradually until the peak stress was reached, and then it appeared as strain softening stage. After the addition of nano-SiO2, the cohesion and internal friction angle of the samples were increased by 8.2 % and 4.1 %, respectively. The viscosity of grouts containing nano-SiO2 increased significantly. By adding 1.5 % super plasticizer, the fluidity of the grouts containing nano-SiO2 could be significantly improved, so as to obtain the grouts with high fluidity and permeability.
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- 2020
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36. Activation of NF-κB is a critical element in the antiapoptotic effect of anesthetic preconditioning
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Lu, Xiyuan, Liu, Hong, Wang, Lianguo, and Schaefer, Saul
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Medical Physiology ,Biomedical and Clinical Sciences ,Heart Disease ,Cardiovascular ,5.1 Pharmaceuticals ,Anesthetics ,Inhalation ,Animals ,Apoptosis ,Caspase 3 ,Cytochromes c ,I-kappa B Kinase ,I-kappa B Proteins ,In Vitro Techniques ,Male ,Methyl Ethers ,Myocardial Reperfusion Injury ,Myocardium ,NF-KappaB Inhibitor alpha ,NF-kappa B ,Perfusion ,Phosphorylation ,Protein Kinase Inhibitors ,Proto-Oncogene Proteins c-bcl-2 ,Rats ,Rats ,Sprague-Dawley ,Reactive Oxygen Species ,Recovery of Function ,Sevoflurane ,Thiophenes ,Ventricular Function ,Left ,Ventricular Pressure ,nuclear factor-kappa B ,reactive oxygen species ,Physiology ,Cardiovascular System & Hematology ,Cardiovascular medicine and haematology ,Medical physiology - Abstract
Anesthetic preconditioning (APC), defined as brief exposure to inhalational anesthetics before cardiac ischemia-reperfusion (I/R), limits injury in both animal models and in humans. APC can result in the production of reactive oxygen species (ROS), and prior work has shown that APC can modify activation of NF-kappaB during I/R, with consequent reduction in the expression of inflammatory mediators. However, the role of NF-kappaB activation before I/R is unknown. Therefore, these experiments tested the hypothesis that APC-induced ROS results in activation of NF-kappaB before I/R, with consequent increased expression of antiapoptotic proteins such as Bcl-2 and decreased apoptosis. Experiments utilized an established perfused heart rat model of sevoflurane APC and I/R. The role of NF-kappaB was defined by a novel method of transient inhibition of the regulatory kinase IKK using the reversible inhibitor SC-514. In addition to functional measures of left ventricular developed and end-diastolic pressure, phosphorylation of IkappaBalpha and activation of NF-kappaB were measured along with cytosolic protein content of Bcl-2, release of cytochrome c, and degradation of caspase-3. APC resulted in ROS-dependent phosphorylation of IkappaBalpha and activation of NF-kappaB before I/R. APC also increased the expression of Bcl-2 before I/R. In addition to functional protection following I/R, APC resulted in lower release of cytochrome c and caspase-3 degradation. These protective effects of APC were abolished by transient inhibition of IkappaBalpha phosphorylation and NF-kappaB activation by SC-514 followed by washout. ROS-dependent activation of NF-kappaB by APC before I/R is a critical element in the protective effect of APC. APC reduces apoptosis and functional impairment by increasing Bcl-2 expression before I/R. Interventions that increase NF-kappaB activation before I/R should protect hearts from I/R injury.
- Published
- 2009
37. Combined Ferromagnetic Nanoparticles for Effective Periodontal Biofilm Eradication in Rat Model
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Tong, Fei, primary, Wang, Pei, additional, Chen, Ziqiang, additional, Liu, Yifan, additional, Wang, Lianguo, additional, Guo, Jun, additional, Li, Zhihua, additional, Cai, Hu, additional, and Wei, Junchao, additional
- Published
- 2023
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38. Sine Cosine Algorithm for Elite Individual Collaborative Search and Its Application in Mechanical Optimization Designs.
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Tang, Junjie and Wang, Lianguo
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- *
OPTIMIZATION algorithms , *COSINE function , *TANGENT function , *ALGORITHMS , *HYPERBOLIC functions , *SEARCH algorithms - Abstract
To address the shortcomings of the sine cosine algorithm such as the low search accuracy, slow convergence speed, and easily falling into local optimality, a sine cosine algorithm for elite individual collaborative search was proposed. Firstly, tent chaotic mapping was used to initialize the population and the hyperbolic tangent function was applied non-linearly to adjust the parameters of the sine cosine algorithm, which enhanced the uniformity of population distribution and balanced the global exploration and local exploitation ability. Secondly, the search method of the sine cosine algorithm was improved by combining the search strategy of the sine cosine algorithm, the m-neighborhood locally optimal individual-guided search strategy, and the global optimal individual-guided search strategy, and, then, the three search strategies were executed alternately, which achieved collaboration, improved the convergence accuracy, and prevented the algorithm from falling into local optima. Finally, a greedy selection strategy was employed to select the best individuals for the population, which accelerated the convergence speed of the sine cosine algorithm. The simulation results illustrated that the sine cosine algorithm for elite individual collaborative search demonstrated a better optimization performance than the sine cosine algorithm, the other improved sine cosine algorithms, the other chaos-based algorithms, and other intelligent optimization algorithms. In addition, the feasibility and applicability of the sine cosine algorithm for elite individual collaborative search were further demonstrated by two mechanical optimization design experiments. [ABSTRACT FROM AUTHOR]
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- 2023
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39. Sevoflurane Preconditioning Limits Intracellular/Mitochondrial Ca2+ in Ischemic Newborn Myocardium
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Liu, Hong, Wang, Lianguo, Eaton, Matt, and Schaefer, Saul
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Medical Physiology ,Biomedical and Clinical Sciences ,Cardiovascular ,Heart Disease - Coronary Heart Disease ,Heart Disease ,Anesthetics ,Inhalation ,Animals ,Animals ,Newborn ,Calcium ,Ischemic Preconditioning ,Myocardial ,Magnetic Resonance Imaging ,Methyl Ethers ,Mitochondria ,Heart ,Myocardial Ischemia ,Myocardial Reperfusion Injury ,Myocardium ,Phosphates ,Potassium Channels ,Rabbits ,Sevoflurane ,Spectrometry ,Fluorescence ,Spectrophotometry ,Ultraviolet ,Clinical Sciences ,Neurosciences ,Anesthesiology ,Clinical sciences - Abstract
UnlabelledSevoflurane preconditioning (SPC) in adult hearts reduces myocardial ischemia/reperfusion (I/R) injury, an effect that may be mediated by reductions in intracellular Ca(2+) ([Ca(2+)](i)) and/or mitochondrial Ca(2+) ([Ca(2+)](m)) accumulation during ischemia and reperfusion. Because the physiology, pharmacology, and metabolic responses of the newborn differ from adults, we tested the hypothesis that SPC protects newborn myocardium by limiting [Ca(2+)](i) and [Ca(2+)](m) by a K(ATP) channel-dependent mechanism. Fluorescence spectrofluorometry and nuclear magnetic resonance spectroscopy were used to measure [Ca(2+)](i), [Ca(2+)](m), and adenosine triphosphate (ATP) in 4- to 7-day-old Langendorff-perfused rabbit hearts. Three experimental groups were used to study the effect of SPC on [Ca(2+)](m)/[Ca(2+)](i), ATP, as well as hemodynamics and ischemic injury. The role of mitochondrial K(ATP) channels was assessed by exposing the SPC hearts to the mitochondrial K(ATP) channel blocker 5-hydroxydecanoic acid. Our results show that SPC significantly decreased [Ca(2+)](i) and [Ca(2+)](m) during I/R, as well as decreased creatine kinase release during reperfusion and resulted in higher ATP. 5-Hydroxydecanoic acid abolished the effect of SPC on [Ca(2+)], hemodynamics, ATP, and creatine kinase release. In conclusion, decreased [Ca(2+)](i) and [Ca(2+)](m) observed with SPC is associated with greater ATP recovery as well as diminished cell injury. Mitochondrial K(ATP) channel blockade attenuates the SPC effect during I/R, suggesting that these channels are involved in the protective effects of SPC in the newborn.ImplicationsThe results of this study support the hypothesis that sevoflurane preconditioning protects newborn hearts from calcium overload and ischemic injury via a mechanism dependent on mitochondrial KATP channels.
- Published
- 2005
40. Experimental Study on Water-Rock Interaction Characteristics of Unconsolidated Sandstone during CO2 Multicomponent Thermal Fluid Injection
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Guo, Mingzhe, primary, Lun, Zengmin, additional, Zhou, Bing, additional, Wang, Lianguo, additional, and Liu, Huiqing, additional
- Published
- 2023
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41. Experimental Study on Mechanical and Damage Evolution Characteristics of Coal during True Triaxial Cyclic Loading and Unloading
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Jiang, Chongyang, primary, Wang, Lianguo, additional, Ding, Ke, additional, Wang, Shuai, additional, Ren, Bo, additional, and Guo, Jiaxing, additional
- Published
- 2023
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42. Sex differences in electrophysiology of the intact atria
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Smith, Charlotte E.R., primary, Guevara, Amanda M., additional, Wang, Lianguo, additional, Grandi, Eleonora, additional, and Ripplinger, Crystal M., additional
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- 2023
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43. Combined Ferromagnetic Nanoparticles for Effective Periodontal Biofilm Eradication in Rat Model
- Author
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Tong,Fei, Wang,Pei, Chen,Ziqiang, Liu,Yifan, Wang,Lianguo, Guo,Jun, Li,Zhihua, Cai,Hu, Wei,Junchao, Tong,Fei, Wang,Pei, Chen,Ziqiang, Liu,Yifan, Wang,Lianguo, Guo,Jun, Li,Zhihua, Cai,Hu, and Wei,Junchao
- Abstract
Fei Tong,1â 4 Pei Wang,1,3,4 Ziqiang Chen,1,3,4 Yifan Liu,1,3,4 Lianguo Wang,1,3,4 Jun Guo,1,3,4 Zhihua Li,1,3,4 Hu Cai,2,4 Junchao Wei1â 4 1School of Stomatology, Nanchang University, Nanchang, 330006, Peopleâs Republic of China; 2School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, Jiangxi Province, 330031, Peopleâs Republic of China; 3The Key Laboratory of Oral Biomedicine, Nanchang, Jiangxi Province, 330006, Peopleâs Republic of China; 4Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang, 330006, Peopleâs Republic of ChinaCorrespondence: Hu Cai, School of Chemistry and Chemical Engineering, Nanchang University, 999# Xuefu Road, Honggutan District, Nanchang, Jiangxi, 330031, Peopleâs Republic of China, Tel +86 791 83969514, Email caihu@ncu.edu.cn Junchao Wei, School of Stomatology, Nanchang University, 49# Fuzhou Road, Donghu District, Nanchang, Jiangxi, 330006, Peopleâs Republic of China, Tel +86 791 86236950, +86 791 6361141, Email weijunchao@ncu.edu.cnIntroduction: The critical challenge for periodontitis therapy is thoroughly eliminating the dental plaque biofilm, particularly penetrating the deep periodontal tissue. Regular therapeutic strategies are insufficient to penetrate the plaque without disturbing the commensal microflora of the oral cavity. Here, we constructed a Fe3O4 magnetic nanoparticle loading minocycline (FPM NPs) to penetrate the biofilm physically and effectively eliminate periodontal biofilm.Methods: In order to penetrate and remove the biofilm effectively, Fe3O4 magnetic nanoparticles were modified with minocycline using a co-precipitation method. The particle size and dispersion of the nanoparticles were characterized by transmission electron microscopy, scanning electron microscopy, and dynamic light scattering. The antibacterial effects were examined to verify the magnetic targeting of FPM NPs. Confocal laser scanning microscopy was employed to check the effect of
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- 2023
44. Experimental investigation on True Triaxial Deformation and Progressive Damage Behaviour of Sandstone
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Li, Zhaolin, Wang, Lianguo, Lu, Yinlong, Li, Wenshuai, Wang, Kai, and Fan, Hao
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- 2019
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45. Developing a Novel Enamel Adhesive with Amorphous Calcium Phosphate and Silver Nanoparticles to Prevent Demineralization during Orthodontic Treatment
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Jia, Ao, primary, Wang, Pei, additional, Tong, Fei, additional, Chen, Ziqiang, additional, Deng, Yunyun, additional, Yao, Haiyan, additional, Wang, Lianguo, additional, Liu, Yifan, additional, and Ge, Hongshan, additional
- Published
- 2023
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46. Design and Verification of Scientific Exploration Mode of Zhurong Mars Rover
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WANG, Lianguo, primary, ZHU, Yan, additional, ZHANG, Baoming, additional, SHEN, Weihua, additional, LI, Xue, additional, XUE, Bin, additional, LI, Yuxi, additional, XU, Weiming, additional, SUN, Shuquan, additional, CAI, Zhiguo, additional, and WANG, Wei, additional
- Published
- 2023
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47. Design of a Highly Adaptable Advance Support for a Deep, Fully Mechanized Roadway and Analysis of Its Support Performance
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Ding, Ke, primary, Wang, Lianguo, additional, Tian, Jiansheng, additional, Ren, Bo, additional, Jiang, Chongyang, additional, and Wang, Shuai, additional
- Published
- 2022
- Full Text
- View/download PDF
48. Evaluation of Thermophysical and Mechanical Properties of Sandstone Due to High-Temperature
- Author
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Dong, Zhen, primary, Chen, Yanpeng, additional, Wang, Xinggang, additional, Kong, Lingfeng, additional, Wang, Lianguo, additional, Li, Xinning, additional, Sun, Fenjin, additional, Ding, Ke, additional, Wu, Hanqi, additional, Chen, Shanshan, additional, and Zhang, Mengyuan, additional
- Published
- 2022
- Full Text
- View/download PDF
49. Experimental study of the influence of water and temperature on the mechanical behavior of mudstone and sandstone
- Author
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Lu, Yinlong, Wang, Lianguo, Sun, Xiaokang, and Wang, Jun
- Published
- 2017
- Full Text
- View/download PDF
50. Enhancing the Efficiency of Mild-Temperature Photothermal Therapy for Cancer Assisting with Various Strategies
- Author
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Wang, Pei, primary, Chen, Biaoqi, additional, Zhan, Yunyan, additional, Wang, Lianguo, additional, Luo, Jun, additional, Xu, Jia, additional, Zhan, Lilin, additional, Li, Zhihua, additional, Liu, Yuangang, additional, and Wei, Junchao, additional
- Published
- 2022
- Full Text
- View/download PDF
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