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2. Gene Expression of Fresh and Frozen/Thawed Canine Embryos

Author

Guaitolini, C Renato de Freitas, Maziero, R. R.D., Castilho, A Cesar de Souza, Derussi, A. A.P., Volpato, R. [UNESP], Ackermann, C. L. [UNESP], Matsubara, L. M. [UNESP], Lopes, M. D. [UNESP], Paranaense University, Oeste Paulista University, and Universidade Estadual Paulista (Unesp)

Abstract

Made available in DSpace on 2021-06-25T11:00:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 BACKGROUND: Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE: The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha-1 and beta-1 and LIFr in canine embryos obtained in vivo and after freezing. MATERIALS AND METHODS: For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS: Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSION: We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha-1 and beta-1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation. Paranaense University Oeste Paulista University Department of Radiology and Animal Reproduction FMVZ/UNESP Department of Radiology and Animal Reproduction FMVZ/UNESP

Published
2020

3. Gender and 5-years course of psychosis patients: focus on clinical and social variables

Author

Comacchio, C, Lasalvia, A, Bonetto, C, Cristofalo, D, Miglietta, E, Petterlini, S, De Santi, K, Tosato, S, Riolo, R, Cremonese, C, Ceccato, E, Zanatta, G, Ruggeri, M, Tansella, M, Bertani, M, Brambilla, P, Marrella, G, Bissoli, S, Perlini, C, Tito, P, Lunardon, M, Gava, F, Borso, E, Grandina, L, Paliotto, M, Roggia, L, Danieli, A, Poloni, C, Altiero, Mr, Piazza, F, Busana, C, Campi, A, Zanconato, A, Zamorani, P, Binotto, R, Caneva, A, Lazzarin, E, Zordan, G, Dolce, C, Fanchin, Gb, Negro, C, Gardellin, F, Crestale, M, Paiola, L, Sale, A, Morandin, I, Biondi, E, Favaretto, Acg, Geatti, S, Urbani, P, De Rossi, M, Spessotto, J, Penelope, R, Grando, L, Sgnaolin, M, Tozzini, C, Visentin, G, Schiavon, L, Gentile, B, Bolacchi, Mg, Marzotto, L, Moni, F, Rossi, L, Amalric, I, Miceli, C, De Zordo, Mr, Ramon, L, Russo, S, Rossi, R, Casagrande, G, De Nardo, V, Facchetti, A, Ramaciotti, F, Marangon, V, Coppola, G, Marcolin, A, Meneghini, P, Sbraccia, F, Segato, C, Cappellari, L, Cutugno, M, Meneghetti, L, Longhin, L, Paoleschi, B, Scalabrin, D, Antonello, L, Purgato, A, Santucci, G, Tosin, C, Volpato, R, Zurlo, R, Zucchetto, M, Pedron, M, Pinton, S, Benetazzo, M, Pavan, L, Semenzin, M, Sifari, L, Zorzi, F, Martucci, Mm, Magno, N, Meloni, G, Toniolo, E, Pavanati, M, Destro, E, Finotti, L, Fiorio, R, Marsilio, A, Pedrocco, N, Pollola, P, Lazzarotto, L, Nose, F, Rossin, P, Vivenza, V, Mazzoncini, R, Urbani, Andrea, Bianchi, L, Carcereri, G, Lunardi, L, Migliorini, G, Perdona, G, Piazza, C, Lamonaca, D, D'Agostini, G, Boggian, I, Piccione, G, Saladini, E, Gomez, F, Frazzingaro, S, Nicolaou, S, Cordioli, L, Bertolazzi, G, Pagliuca, V, Abate, M, Bortolomasi, M, Giacopuzzi, M, Segala, M, De Nardi, F, Basetto, F, Bernardis, C, Bezzetto, A, and Santi, M

Subjects
Adult, Male, Mental Health Services, First episode psychosis, Psychosis, medicine.medical_specialty, Time Factors, Adolescent, FEP, Gender, Insight, Needs, Psychopathology, Unmet needs, Cohort Studies, Young Adult, 03 medical and health sciences, Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA, Sex Factors, 0302 clinical medicine, Female patient, medicine, Humans, Psychiatry, Depressive symptoms, Demography, Psychiatric Status Rating Scales, Health Services Needs and Demand, business.industry, Obstetrics and Gynecology, Middle Aged, medicine.disease, 030227 psychiatry, Psychiatry and Mental health, Insight into illness, Italy, Psychotic Disorders, Male patient, Female, business, Social Adjustment, 030217 neurology & neurosurgery, Follow-Up Studies
Abstract

Most studies on gender and psychosis have focused on gender differences at illness onset or on the long-term outcome, whereas little is known about the impact of gender on the first years after psychosis onset. A total of 185 first episode psychosis (FEP) patients were followed for 5 years after psychosis onset, and gender differences were explored in psychopathology (PANSS), needs for care (CAN), and insight (SAI-E). Male patients showed more negative symptoms than females over time, whereas female patients showed higher levels of depressive symptoms than males throughout the study period. In addition, female patients presented more functioning unmet needs for care, but higher levels of insight into illness than males. Therapy and rehabilitative programs for FEP patients should be gender-targeted, as gender has proved to impact on psychopathology, needs for care, and insight in the very first years following psychosis onset.

Published
2019
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4. Gender and 5-years course of psychosis patients: focus on clinical and social variables

Author

Comacchio, C, Lasalvia, A, Bonetto, C, Cristofalo, D, Miglietta, E, Petterlini, S, De Santi, K, Tosato, S, Riolo, R, Cremonese, C, Ceccato, E, Zanatta, G, Ruggeri, M, Tansella, M, Bertani, M, Brambilla, P, Marrella, G, Bissoli, S, Perlini, C, Tito, P, Lunardon, M, Gava, F, Borso, E, Grandina, L, Paliotto, M, Roggia, L, Danieli, A, Poloni, C, Altiero, Mr, Piazza, F, Busana, C, Campi, A, Zanconato, A, Zamorani, P, Binotto, R, Caneva, A, Lazzarin, E, Zordan, G, Dolce, C, Fanchin, Gb, Negro, C, Gardellin, F, Crestale, M, Paiola, L, Sale, A, Morandin, I, Biondi, E, Favaretto, Acg, Geatti, S, Urbani, P, De Rossi, M, Spessotto, J, Penelope, R, Grando, L, Sgnaolin, M, Tozzini, C, Visentin, G, Schiavon, L, Gentile, B, Bolacchi, Mg, Marzotto, L, Moni, F, Rossi, L, Amalric, I, Miceli, C, De Zordo, Mr, Ramon, L, Russo, S, Rossi, R, Casagrande, G, De Nardo, V, Facchetti, A, Ramaciotti, F, Marangon, V, Coppola, G, Marcolin, A, Meneghini, P, Sbraccia, F, Segato, C, Cappellari, L, Cutugno, M, Meneghetti, L, Longhin, L, Paoleschi, B, Scalabrin, D, Antonello, L, Purgato, A, Santucci, G, Tosin, C, Volpato, R, Zurlo, R, Zucchetto, M, Pedron, M, Pinton, S, Benetazzo, M, Pavan, L, Semenzin, M, Sifari, L, Zorzi, F, Martucci, Mm, Magno, N, Meloni, G, Toniolo, E, Pavanati, M, Destro, E, Finotti, L, Fiorio, R, Marsilio, A, Pedrocco, N, Pollola, P, Lazzarotto, L, Nose, F, Rossin, P, Vivenza, V, Mazzoncini, R, Urbani, Andrea, Bianchi, L, Carcereri, G, Lunardi, L, Migliorini, G, Perdona, G, Piazza, C, Lamonaca, D, D'Agostini, G, Boggian, I, Piccione, G, Saladini, E, Gomez, F, Frazzingaro, S, Nicolaou, S, Cordioli, L, Bertolazzi, G, Pagliuca, V, Abate, M, Bortolomasi, M, Giacopuzzi, M, Segala, M, De Nardi, F, Basetto, F, Bernardis, C, Bezzetto, A, Santi, M, Urbani, A (ORCID:0000-0001-9168-3174), Comacchio, C, Lasalvia, A, Bonetto, C, Cristofalo, D, Miglietta, E, Petterlini, S, De Santi, K, Tosato, S, Riolo, R, Cremonese, C, Ceccato, E, Zanatta, G, Ruggeri, M, Tansella, M, Bertani, M, Brambilla, P, Marrella, G, Bissoli, S, Perlini, C, Tito, P, Lunardon, M, Gava, F, Borso, E, Grandina, L, Paliotto, M, Roggia, L, Danieli, A, Poloni, C, Altiero, Mr, Piazza, F, Busana, C, Campi, A, Zanconato, A, Zamorani, P, Binotto, R, Caneva, A, Lazzarin, E, Zordan, G, Dolce, C, Fanchin, Gb, Negro, C, Gardellin, F, Crestale, M, Paiola, L, Sale, A, Morandin, I, Biondi, E, Favaretto, Acg, Geatti, S, Urbani, P, De Rossi, M, Spessotto, J, Penelope, R, Grando, L, Sgnaolin, M, Tozzini, C, Visentin, G, Schiavon, L, Gentile, B, Bolacchi, Mg, Marzotto, L, Moni, F, Rossi, L, Amalric, I, Miceli, C, De Zordo, Mr, Ramon, L, Russo, S, Rossi, R, Casagrande, G, De Nardo, V, Facchetti, A, Ramaciotti, F, Marangon, V, Coppola, G, Marcolin, A, Meneghini, P, Sbraccia, F, Segato, C, Cappellari, L, Cutugno, M, Meneghetti, L, Longhin, L, Paoleschi, B, Scalabrin, D, Antonello, L, Purgato, A, Santucci, G, Tosin, C, Volpato, R, Zurlo, R, Zucchetto, M, Pedron, M, Pinton, S, Benetazzo, M, Pavan, L, Semenzin, M, Sifari, L, Zorzi, F, Martucci, Mm, Magno, N, Meloni, G, Toniolo, E, Pavanati, M, Destro, E, Finotti, L, Fiorio, R, Marsilio, A, Pedrocco, N, Pollola, P, Lazzarotto, L, Nose, F, Rossin, P, Vivenza, V, Mazzoncini, R, Urbani, Andrea, Bianchi, L, Carcereri, G, Lunardi, L, Migliorini, G, Perdona, G, Piazza, C, Lamonaca, D, D'Agostini, G, Boggian, I, Piccione, G, Saladini, E, Gomez, F, Frazzingaro, S, Nicolaou, S, Cordioli, L, Bertolazzi, G, Pagliuca, V, Abate, M, Bortolomasi, M, Giacopuzzi, M, Segala, M, De Nardi, F, Basetto, F, Bernardis, C, Bezzetto, A, Santi, M, and Urbani, A (ORCID:0000-0001-9168-3174)

Abstract

Most studies on gender and psychosis have focused on gender differences at illness onset or on the long-term outcome, whereas little is known about the impact of gender on the first years after psychosis onset. A total of 185 first episode psychosis (FEP) patients were followed for 5 years after psychosis onset, and gender differences were explored in psychopathology (PANSS), needs for care (CAN), and insight (SAI-E). Male patients showed more negative symptoms than females over time, whereas female patients showed higher levels of depressive symptoms than males throughout the study period. In addition, female patients presented more functioning unmet needs for care, but higher levels of insight into illness than males. Therapy and rehabilitative programs for FEP patients should be gender-targeted, as gender has proved to impact on psychopathology, needs for care, and insight in the very first years following psychosis onset.

Published
2020

5. The Gravity Dual of the Ising Model

Author

Castro, A., Gaberdiel, M., Hartman, T., Maloney, A., and Volpato, R.

Subjects
High Energy Physics - Theory, High Energy Physics - Theory (hep-th), FOS: Physical sciences
Abstract

We evaluate the partition function of three dimensional theories of gravity in the quantum regime, where the AdS radius is Planck scale and the central charge is of order one. The contribution from the AdS vacuum sector can - with certain assumptions - be computed and equals the vacuum character of a minimal model CFT. The torus partition function is given by a sum over geometries which is finite and computable. For generic values of Newton's constant G and the AdS radius L the result has no Hilbert space interpretation, but in certain cases it agrees with the partition function of a known CFT. For example, the partition function of pure Einstein gravity with G=3L equals that of the Ising model, providing evidence that these theories are dual. We also present somewhat weaker evidence that the 3-state and tricritical Potts models are dual to pure higher spin theories of gravity based on SL(3) and E_6, respectively., Comment: 42 pages

Published
2011
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9. Ressonância magnética na avaliação das articulações do pé de pacientes com artrite reumatoide

Author

RONDINA, R. G., VOLPATO, R., Rosa Júnior M, NACIF, M. S., and MELLO, R. A. F.

Abstract

Made available in DSpace on 2018-08-01T23:27:40Z (GMT). No. of bitstreams: 1 tese_9947_Final - Dissertacao.pdf: 14419652 bytes, checksum: b7d4e8bad58f7c3a3fcd93f9a0c6836f (MD5) Previous issue date: 2016-06-24 Objetivo: Estudar as articulações do pé clinicamente dominante de pacientes com artrite reumatoide por meio de exames de RM utilizando o escore RAMRIS e correlacionar com os dados clínicos relacionados à atividade da doença e capacidade funcional, avaliados respectivamente pelos escores DAS28 e HAQ. Métodos: Foi realizado um estudo transversal e descritivo com cinquenta e cinco pacientes com diagnóstico de artrite reumatoide acompanhados pelo ambulatório de Reumatologia do Hospital Cassiano Antônio de Moraes (HUCAM) da Universidade Federal do Espírito Santo (UFES) entre fevereiro e dezembro de 2014. Foi realizadaavaliação do pé clinicamente dominante pela ressonância magnética, bem como foram coletados dados clínicos e laboratoriais para mensuração dos escores DAS28 e HAQ. Resultados: Nosso estudo demostrou uma relação linear entre grau de acometimento articular, avaliado pelo RAMRIS, com a perda de capacidade funcional, avaliada pelo questionário HAQ. Também houve relação linear entre o escore que avalia a atividade da doença (DAS28) e o HAQ. Não houve relação estatística entre o grau de acometimento articular, avaliado pelo RAMRIS, com o escore DAS28. Conclusão: Concluímos que a avaliação por RM pode documentar um estado de doença persistentemente ativa apesar da remissão clínica avaliada pelo escore DAS28. Palavras-chaves: Artrite Reumatoide; Pés; Remissão; Ressonância magnética.

Published
2016

10. A rede padrão de repouso parece preservada intrinsecamente mas sua conectividade funcional extrínseca pode estar comprometida em usuários de crack-cocaína

Author

MARTINS, D. L. N., VOLPATO, R., Rosa Júnior M, MELLO, R. A. F., PACHECO, F. T., and NAKAMURA-PALACIOS, E. M.

Abstract

Made available in DSpace on 2018-08-01T23:27:39Z (GMT). No. of bitstreams: 1 tese_9946_Diego Lima Nava Martins - Dissertação MPM 2016 (final).pdf: 10579151 bytes, checksum: e482b2a50783a970f9fb4cb3fcb92886 (MD5) Previous issue date: 2016-06-17 O Defalut Mode Network (DMN) parace estar afetado na dependência química. Conectividade funcional reduzida foi descrita em usuários de psicoestimulantes (cocaína, nicotina e cafeína) e também em dependentes de drogas depressoras (álcool, heroína, opioides de prescrição), porém nenhum estudo investigou a conectividade funcional do DMN em dependentes de crack até então. Neste estudo, usuários de crack em abstinência por no mínimo 4 semanas e pareados por idade com controles não usuários foram submetidos a exames de imagem por Ressonância Magnética funcional adquiridos quando em repouso e com os olhos fechados (rs-fMRI) em aparelhos de 1,5T e 3,0T. Após o pré-processamento dos dados, DMN foram definidos por análise de componentes independentes (Independent Component Analysis ICA) e por análise de correlação seed based, por regiões de interesse (ROIs) no Córtex Cingulado Anterior ventral (vACC) e no Córtex Cingulado Posterior (PCC). A conectividade funcional global do DMN não foi diferente entre os usuários de crack e os controles não usuários pareados por idade nos estudos de rs-fMRI adquiridos em ambos os scanners. A análise seed based evidenciou maior negatividade da conectividade entre o vACC e o lóbulo parietal superior esquerdo quando comparado aos controles pareados por idade (p < 0,0322). Não foram encontradas diferenças entre os grupos na conectividade funcional entre o PCC e outras regiões cerebrais. Assim, a conectividade funcional total do DMN analisada por ICA foi encontrada preservada nos dependentes de crack em abstinência. Quando uma análise de correlação seed based foi aplicada buscando-se por uma conectividade funcional métrica simples entre regiões cerebrais específicas, uma maior negatividade foi encontrada entre a região frontal medial e a região cerebral posterior, sugerindo que embora o DMN global não esteja afetado uma conectividade funcional seletiva pode estar comprometida na dependência do crack. Palavras-chave: Cocaína Crack. Dependência. Transtornos Relacionados ao Uso de Substâncias. Imagem por Ressonância Magnética. IRMf.

Published
2016

11. Postoperative Analgesic Effects of Laserpuncture and Meloxicam in Bitches Submitted to Ovariohysterectomy.

Author

Tomacheuski RM, Taffarel MO, Cardoso GS, Derussi AAP, Ferrante M, Volpato R, and Luna SPL

Abstract

Background: This prospective, randomised and blind study investigated the efficacy of laserpuncture for postoperative pain management in dogs., Method: Sixteen bitches were sedated with acepromazine and randomly treated before ovariohysterectomy with meloxicam 0.2 mg·kg -1 intramuscular or laserpuncture (wavelength 904 mm, frequency 124 Hz, potency 10 Joules, 100 s in each acupoint). Anaesthesia was performed with propofol, isoflurane/O 2 , and fentanyl. The Glasgow Composite Measure Pain Scale (GCMPS) and Dynamic Interactive Visual Analog Scale (DIVAS) were used to evaluate postoperative pain before and for 24 h after surgery. Morphine was administrated as rescue analgesia when pain scores were ≥3.33 (GCMPS). Differences between treatments, time points, and amount of rescue analgesia between groups were investigated by the Mann-Whitney test and the area under the curve (AUC) for GCMPS, Friedman, and Chi-squared tests, respectively ( p < 0.05)., Results: Dogs treated with laserpuncture presented lower GCMPS AUC for 24 h and lower GCMPS scores at 2 and 4 h postoperatively ( p = 0.04). Three dogs treated with meloxicam required postoperatively rescue analgesia against none treated with laserpuncture., Conclusions: In this preliminary study, laserpuncture mitigated postoperative pain in dogs following ovariohysterectomy, and the technique is a promising adjunct to perioperative pain management in dogs undergoing soft tissue surgery.

Published
2020
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12. Partial ablation of endometrial glands in dogs after exposure to progestin during the neonatal period.

Author

Teixeira NS, Martins BB, Volpato R, Ramos JLG, Freitas PMC, Laufer-Amorim R, Lopes MD, and Luz MR

Abstract

Bitches with uteri devoid of endometrial glands should be sterile, and consequently could contribute to the population control of dogs. Considering that an inadequate exposure of the female reproductive system to steroids can lead to the formation of the uterine gland knock-out (UGKO) phenotype in some species, the aim of this study was to evaluate the effect of serial applications of medroxyprogesterone acetate (MPA) from birth until the age of six months on the development of endometrial glands in bitches. For this purpose, 16 female mongrel dogs from different litters were distributed into either an MPA group (n = 8), animals treated with 10 mg kg sc (Promone-E ® , Pfizer, Brasil) at 3-week intervals, from day one after birth until the age of six months, or a control group (n = 8), composed of animals that only received a 0.9% NaCl solution in place of MPA. At six months of age, ovariohysterectomy was performed and uterine horn samples were collected for histological and immunohistochemical examinations. The bitches from the MPA-treated group presented a 35% decrease in the number of endometrial glands, a larger diameter of the endometrial glands, a greater epithelial height, as well as a greater thickness of the uterine wall, endometrium, and myometrium. However, no significant differences were observed between the two groups in the expression of ER-α, ER-β, and PR on the surface epithelium and endometrial stroma. Therefore, the serial application of MPA from birth until the age of 6 months do not completely ablate the development of the endometrial glands in bitches, but impair it by 35%., Competing Interests: The authors declare no conflicts of interest.

Published
2018
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13. Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats.

Author

Ackermann CL, Trevisol E, Crocomo LF, Rascado TDS, Volpato R, Guaitolini CRF, Lopes C, Costa TA, and Lopes MD

Subjects
Animals, Blastocyst cytology, Blastocyst physiology, Cats, Female, Fertilization in Vitro methods, Oocyte Retrieval methods, Oocytes cytology, Triptorelin Pamoate pharmacology, Fertilization in Vitro veterinary, Oocyte Retrieval veterinary, Oocytes drug effects, Triptorelin Pamoate analogs & derivatives
Abstract

Objectives The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. Methods Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group. After 6 months of treatment, all animals were ovariohysterectomized, and the ovaries were used for in vitro embryo production. Percentage of cleavage was determined 18 h after oocyte insemination and blastocyst formation was assessed on the eighth day of culture. The rate of cumulus-oocyte complexes (COCs) recovery was analyzed by an unpaired t-test. The cleavage and blastocyst rates were expressed as percentages and analyzed by Fisher's exact test. All analyses were performed using GraphPad Prism v5.0, with P <0.05 set as the level of significance. Results In the treated group, we recovered 8.3 ± 1.15 grade I COCs per queen; the cleavage rate was 60% and the blastocyst rate was 36%. In the control group, we recovered 18.4 ± 3.21 grade I COCs per queen; the cleavage rate was 55.97% and the blastocyst rate was 34%. Forty percent of treated females did not produce any blastocysts. In the treated group, we observed a significant decrease in COC recovery. Although there was no significant difference in cleavage and blastocyst rates between groups, 40% of treated females did not produce any blastocysts. Conclusions Recovery of grade I COCs is negatively affected by deslorelin treatment in domestic cats. Regarding embryo production, new studies are still necessary to evaluate the success of this technique owing to the individual effect caused by deslorelin acetate.

Published
2017
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15. Diabetes mellitus, hypercholesterolemia, and hypertension but not vascular disease per se are associated with persistent platelet activation in vivo. Evidence derived from the study of peripheral arterial disease.

Author

Davì G, Gresele P, Violi F, Basili S, Catalano M, Giammarresi C, Volpato R, Nenci GG, Ciabattoni G, and Patrono C

Subjects
Adult, Aged, Aspirin administration & dosage, Cross-Sectional Studies, Female, Follow-Up Studies, Humans, Male, Middle Aged, Multivariate Analysis, Platelet Activation drug effects, Prospective Studies, Reproducibility of Results, Risk Factors, Smoking adverse effects, Diabetes Mellitus, Type 2 physiopathology, Hypercholesterolemia physiopathology, Hypertension physiopathology, Platelet Activation physiology, Thromboxane A2 biosynthesis, Thromboxane B2 biosynthesis, Vascular Diseases physiopathology
Abstract

Background: Previous studies relating increased thromboxane (TX) biosynthesis to cardiovascular risk factors do not answer the question whether platelet activation is merely a consequence of more prevalent atherosclerotic lesions or reflects the influence of metabolic and hemodynamic disturbances on platelet biochemistry and function., Methods and Results: We examined 64 patients with large-vessel peripheral arterial disease and 64 age- and sex-matched control subjects. TXA2 biosynthesis was investigated in relation to cardiovascular risk factors by repeated measurements of the urinary excretion of its major enzymatic metabolite, 11-dehydro-TXB2, by radioimmunoassay. Urinary 11-dehydro-TXB2 was significantly (P = .0001) higher in patients with peripheral arterial disease (57 +/- 26 ng/h) than in control subjects (26 +/- 7 ng/h). Seventy percent of patients had metabolite excretion > 2 SD above the normal mean. However, 11-dehydro-TXB2 excretion was enhanced only in association with cardiovascular risk factors. Multivariate analysis showed that diabetes, hypercholesterolemia, and hypertension were independently related to 11-dehydro-TXB2 excretion. During a median follow-up of 48 months, 8 patients experienced major vascular events. These patients had significantly (P = .001) higher 11-dehydro-TXB2 excretion at baseline than patients who remained event free., Conclusions: The occurrence of large-vessel peripheral arterial disease per se is not a trigger of platelet activation in vivo. Rather, the rate of TXA2 biosynthesis appears to reflect the influence of coexisting disorders such as diabetes mellitus, hypercholesterolemia, and hypertension on platelet biochemistry and function. Enhanced TXA2 biosynthesis may represent a common link between such diverse risk factors and the thrombotic complications of peripheral arterial disease.

Published
1997
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