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1. 30-day morbidity and mortality of sleeve gastrectomy, Roux-en-Y gastric bypass and one anastomosis gastric bypass: a propensity score-matched analysis of the GENEVA data

Author: Singhal R., Cardoso V. R., Wiggins T., Super J., Ludwig C., Gkoutos G. V., Mahawar K., Pedziwiatr M., Major P., Zarzycki P., Pantelis A., Lapatsanis D. P., Stravodimos G., Matthys C., Focquet M., Vleeschouwers W., Spaventa A. G., Zerrweck C., Vitiello A., Berardi G., Musella M., Sanchez-Meza A., Cantu F. J., Mora F., Cantu M. A., Katakwar A., Reddy D. N., Elmaleh H., Hassan M., Elghandour A., Elbanna M., Osman A., Khan A., layani L., Kiran N., Velikorechin A., Solovyeva M., Melali H., Shahabi S., Agrawal A., Shrivastava A., Sharma A., Narwaria B., Narwaria M., Raziel A., Sakran N., Susmallian S., Karagoz L., Akbaba M., Piskin S. Z., Balta A. Z., Senol Z., Manno E., Iovino M. G., Qassem M., Arana-Garza S., Povoas H. P., Vilas-Boas M. L., Naumann D., Li A., Ammori B. J., Balamoun H., Salman M., Nasta A. M., Goel R., Sanchez-Aguilar H., Herrera M. F., Abou-mrad A., Cloix L., Mazzini G. S., Kristem L., Lazaro A., Campos J., Bernardo J., Gonzalez J., Trindade C., Viveiros O., Ribeiro R., Goitein D., Hazzan D., Segev L., Beck T., Reyes H., Monterrubio J., Garcia P., Benois M., Kassir R., Contine A., Elshafei M., Aktas S., Weiner S., Heidsieck T., Level L., Pinango S., Ortega P. M., Moncada R., Valenti V., Vlahovic I., Boras Z., Liagre A., Martini F., Juglard G., Motwani M., Saggu S. S., Momani H. A., Lopez L. A. A., Cortez M. A. C., Zavala R. A., D'Haese RN C., Kempeneers I., Himpens J., Lazzati A., Paolino L., Bathaei S., Bedirli A., Yavuz A., Buyukkasap C., Ozaydin S., Kwiatkowski A., Bartosiak K., Waledziak M., Santonicola A., Angrisani L., Iovino P., Palma R., Iossa A., Boru C. E., De Angelis F., Silecchia G., Hussain A., Balchandra S., Coltell I. B., Perez J. L., Bohra A., Awan A. K., Madhok B., Leeder P. C., Awad S., Al-Khyatt W., Shoma A., Elghadban H., Ghareeb S., Mathews B., Kurian M., Larentzakis A., Vrakopoulou G. Z., Albanopoulos K., Bozdag A., Lale A., Kirkil C., Dincer M., Bashir A., Haddad A., Hijleh L. A., Zilberstein B., de Marchi D. D., Souza W. P., Broden C. M., Gislason H., Shah K., Ambrosi A., Pavone G., Tartaglia N., Kona S. L. K., Kalyan K., Perez C. E. G., Botero M. A. F., Covic A., Timofte D., Maxim M., Faraj D., Tseng L., Liem R., Oren G., Dilektasli E., Yalcin I., AlMukhtar H., Hadad M. A., Mohan R., Arora N., Bedi D., Rives-Lange C., Chevallier J. -M., Poghosyan T., Sebbag H., Zinai L., Khaldi S., Mauchien C., Mazza D., Dinescu G., Rea B., Perez-Galaz F., Zavala L., Besa A., Curell A., Balibrea J. M., Vaz C., Galindo L., Silva N., Caballero J. L. E., Sebastian S. O., Marchesini J. C. D., da Fonseca Pereira R. A., Sobottka W. H., Fiolo F. E., Turchi M., Coelho A. C. J., Zacaron A. L., Barbosa A., Quinino R., Menaldi G., Paleari N., Martinez-Duartez P., de Esparza G. M. A. R., Esteban V. S., Torres A., Garcia-Galocha J. L., Josa M., Pacheco-Garcia J. M., Mayo-Ossorio M. A., Chowbey P., Soni V., de Vasconcelos Cunha H. A., Castilho M. V., Ferreira R. M. A., Barreiro T. A., Charalabopoulos A., Sdralis E., Davakis S., Bomans B., Dapri G., Van Belle K., Takieddine M., Vaneukem P., Karaca E. S. A., Karaca F. C., Sumer A., Peksen C., Savas O. A., Chousleb E., Elmokayed F., Fakhereldin I., Aboshanab H. M., Swelium T., Gudal A., Gamloo L., Ugale A., Ugale S., Boeker C., Reetz C., Hakami I. A., Mall J., Alexandrou A., Baili E., Bodnar Z., Maleckas A., Gudaityte R., Guldogan C. E., Gundogdu E., Ozmen M. M., Thakkar D., Dukkipati N., Shah P. S., Shah S. S., Adil M. T., Jambulingam P., Mamidanna R., Whitelaw D., Jain V., Veetil D. K., Wadhawan R., Torres M., Tinoco T., Leclercq W., Romeijn M., van de Pas K., Alkhazraji A. K., Taha S. A., Ustun M., Yigit T., Inam A., Burhanulhaq M., Pazouki A., Eghbali F., Kermansaravi M., Jazi A. H. D., Mahmoudieh M., Mogharehabed N., Tsiotos G., Stamou K., Rodriguez F. J. B., Navarro M. A. R., Torres O. M., Martinez S. L., Tamez E. R. M., Cornejo G. A. M., Flores J. E. G., Mohammed D. A., Elfawal M. H., Shabbir A., Guowei K., So J. B., Kaplan E. T., Kaplan M., Kaplan T., Pham D. T., Rana G., Kappus M., Gadani R., Kahitan M., Pokharel K., Osborne A., Pournaras D., Hewes J., Napolitano E., Chiappetta S., Bottino V., Dorado E., Schoettler A., Gaertner D., Fedtke K., Aguilar-Espinosa F., Aceves-Lozano S., Balani A., Nagliati C., Pennisi D., Rizzi A., Frattini F., Foschi D., Benuzzi L., Parikh C., Shah H., Pinotti E., Montuori M., Borrelli V., Dargent J., Copaescu C. A., Hutopila I., Smeu B., Witteman B., Hazebroek E., Deden L., Heusschen L., Okkema S., Aufenacker T., den Hengst W., Vening W., van der Burgh Y., Ghazal A., Ibrahim H., Niazi M., Alkhaffaf B., Altarawni M., Cesana G. C., Anselmino M., Uccelli M., Olmi S., Stier C., Akmanlar T., Sonnenberg T., Schieferbein U., Marcolini A., Awruch D., Vicentin M., de Souza Bastos E. L., Gregorio S. A., Ahuja A., Mittal T., Bolckmans R., Baratte C., Wisnewsky J. A., Genser L., Chong L., Taylor L., Ward S., Hi M. W., Heneghan H., Fearon N., Plamper A., Rheinwalt K., Geoghegan J., Ng K. C., Kaseja K., Kotowski M., Samarkandy T. A., Leyva-Alvizo A., Corzo-Culebro L., Wang C., Yang W., Dong Z., Riera M., Jain R., Hamed H., Said M., Zarzar K., Garcia M., Turkcapar A. G., Sen O., Baldini E., Conti L., Wietzycoski C., Lopes E., Pintar T., Salobir J., Aydin C., Atici S. D., Ergin A., Ciyiltepe H., Bozkurt M. A., Kizilkaya M. C., Onalan N. B. D., Zuber M. N. B. A., Wong W. J., Garcia A., Vidal L., Beisani M., Pasquier J., Vilallonga R., Sharma S., Parmar C., Lee L., Sufi P., Sinan H., Saydam M., Singhal, R., Cardoso, V. R., Wiggins, T., Super, J., Ludwig, C., Gkoutos, G. V., Mahawar, K., Pedziwiatr, M., Major, P., Zarzycki, P., Pantelis, A., Lapatsanis, D. P., Stravodimos, G., Matthys, C., Focquet, M., Vleeschouwers, W., Spaventa, A. G., Zerrweck, C., Vitiello, A., Berardi, G., Musella, M., Sanchez-Meza, A., Cantu, F. J., Mora, F., Cantu, M. A., Katakwar, A., Reddy, D. N., Elmaleh, H., Hassan, M., Elghandour, A., Elbanna, M., Osman, A., Khan, A., Layani, L., Kiran, N., Velikorechin, A., Solovyeva, M., Melali, H., Shahabi, S., Agrawal, A., Shrivastava, A., Sharma, A., Narwaria, B., Narwaria, M., Raziel, A., Sakran, N., Susmallian, S., Karagoz, L., Akbaba, M., Piskin, S. Z., Balta, A. Z., Senol, Z., Manno, E., Iovino, M. G., Qassem, M., Arana-Garza, S., Povoas, H. P., Vilas-Boas, M. L., Naumann, D., Li, A., Ammori, B. J., Balamoun, H., Salman, M., Nasta, A. M., Goel, R., Sanchez-Aguilar, H., Herrera, M. F., Abou-mrad, A., Cloix, L., Mazzini, G. S., Kristem, L., Lazaro, A., Campos, J., Bernardo, J., Gonzalez, J., Trindade, C., Viveiros, O., Ribeiro, R., Goitein, D., Hazzan, D., Segev, L., Beck, T., Reyes, H., Monterrubio, J., Garcia, P., Benois, M., Kassir, R., Contine, A., Elshafei, M., Aktas, S., Weiner, S., Heidsieck, T., Level, L., Pinango, S., Ortega, P. M., Moncada, R., Valenti, V., Vlahovic, I., Boras, Z., Liagre, A., Martini, F., Juglard, G., Motwani, M., Saggu, S. S., Momani, H. A., Lopez, L. A. A., Cortez, M. A. C., Zavala, R. A., D'Haese RN, C., Kempeneers, I., Himpens, J., Lazzati, A., Paolino, L., Bathaei, S., Bedirli, A., Yavuz, A., Buyukkasap, C., Ozaydin, S., Kwiatkowski, A., Bartosiak, K., Waledziak, M., Santonicola, A., Angrisani, L., Iovino, P., Palma, R., Iossa, A., Boru, C. E., De Angelis, F., Silecchia, G., Hussain, A., Balchandra, S., Coltell, I. B., Perez, J. L., Bohra, A., Awan, A. K., Madhok, B., Leeder, P. C., Awad, S., Al-Khyatt, W., Shoma, A., Elghadban, H., Ghareeb, S., Mathews, B., Kurian, M., Larentzakis, A., Vrakopoulou, G. Z., Albanopoulos, K., Bozdag, A., Lale, A., Kirkil, C., Dincer, M., Bashir, A., Haddad, A., Hijleh, L. A., Zilberstein, B., de Marchi, D. D., Souza, W. P., Broden, C. M., Gislason, H., Shah, K., Ambrosi, A., Pavone, G., Tartaglia, N., Kona, S. L. K., Kalyan, K., Perez, C. E. G., Botero, M. A. F., Covic, A., Timofte, D., Maxim, M., Faraj, D., Tseng, L., Liem, R., Oren, G., Dilektasli, E., Yalcin, I., Almukhtar, H., Hadad, M. A., Mohan, R., Arora, N., Bedi, D., Rives-Lange, C., Chevallier, J. -M., Poghosyan, T., Sebbag, H., Zinai, L., Khaldi, S., Mauchien, C., Mazza, D., Dinescu, G., Rea, B., Perez-Galaz, F., Zavala, L., Besa, A., Curell, A., Balibrea, J. M., Vaz, C., Galindo, L., Silva, N., Caballero, J. L. E., Sebastian, S. O., Marchesini, J. C. D., da Fonseca Pereira, R. A., Sobottka, W. H., Fiolo, F. E., Turchi, M., Coelho, A. C. J., Zacaron, A. L., Barbosa, A., Quinino, R., Menaldi, G., Paleari, N., Martinez-Duartez, P., de Esparza, G. M. A. R., Esteban, V. S., Torres, A., Garcia-Galocha, J. L., Josa, M., Pacheco-Garcia, J. M., Mayo-Ossorio, M. A., Chowbey, P., Soni, V., de Vasconcelos Cunha, H. A., Castilho, M. V., Ferreira, R. M. A., Barreiro, T. A., Charalabopoulos, A., Sdralis, E., Davakis, S., Bomans, B., Dapri, G., Van Belle, K., Takieddine, M., Vaneukem, P., Karaca, E. S. A., Karaca, F. C., Sumer, A., Peksen, C., Savas, O. A., Chousleb, E., Elmokayed, F., Fakhereldin, I., Aboshanab, H. M., Swelium, T., Gudal, A., Gamloo, L., Ugale, A., Ugale, S., Boeker, C., Reetz, C., Hakami, I. A., Mall, J., Alexandrou, A., Baili, E., Bodnar, Z., Maleckas, A., Gudaityte, R., Guldogan, C. E., Gundogdu, E., Ozmen, M. M., Thakkar, D., Dukkipati, N., Shah, P. S., Shah, S. S., Adil, M. T., Jambulingam, P., Mamidanna, R., Whitelaw, D., Jain, V., Veetil, D. K., Wadhawan, R., Torres, M., Tinoco, T., Leclercq, W., Romeijn, M., van de Pas, K., Alkhazraji, A. K., Taha, S. A., Ustun, M., Yigit, T., Inam, A., Burhanulhaq, M., Pazouki, A., Eghbali, F., Kermansaravi, M., Jazi, A. H. D., Mahmoudieh, M., Mogharehabed, N., Tsiotos, G., Stamou, K., Rodriguez, F. J. B., Navarro, M. A. R., Torres, O. M., Martinez, S. L., Tamez, E. R. M., Cornejo, G. A. M., Flores, J. E. G., Mohammed, D. A., Elfawal, M. H., Shabbir, A., Guowei, K., So, J. B., Kaplan, E. T., Kaplan, M., Kaplan, T., Pham, D. T., Rana, G., Kappus, M., Gadani, R., Kahitan, M., Pokharel, K., Osborne, A., Pournaras, D., Hewes, J., Napolitano, E., Chiappetta, S., Bottino, V., Dorado, E., Schoettler, A., Gaertner, D., Fedtke, K., Aguilar-Espinosa, F., Aceves-Lozano, S., Balani, A., Nagliati, C., Pennisi, D., Rizzi, A., Frattini, F., Foschi, D., Benuzzi, L., Parikh, C., Shah, H., Pinotti, E., Montuori, M., Borrelli, V., Dargent, J., Copaescu, C. A., Hutopila, I., Smeu, B., Witteman, B., Hazebroek, E., Deden, L., Heusschen, L., Okkema, S., Aufenacker, T., den Hengst, W., Vening, W., van der Burgh, Y., Ghazal, A., Ibrahim, H., Niazi, M., Alkhaffaf, B., Altarawni, M., Cesana, G. C., Anselmino, M., Uccelli, M., Olmi, S., Stier, C., Akmanlar, T., Sonnenberg, T., Schieferbein, U., Marcolini, A., Awruch, D., Vicentin, M., de Souza Bastos, E. L., Gregorio, S. A., Ahuja, A., Mittal, T., Bolckmans, R., Baratte, C., Wisnewsky, J. A., Genser, L., Chong, L., Taylor, L., Ward, S., Hi, M. W., Heneghan, H., Fearon, N., Plamper, A., Rheinwalt, K., Geoghegan, J., Ng, K. C., Kaseja, K., Kotowski, M., Samarkandy, T. A., Leyva-Alvizo, A., Corzo-Culebro, L., Wang, C., Yang, W., Dong, Z., Riera, M., Jain, R., Hamed, H., Said, M., Zarzar, K., Garcia, M., Turkcapar, A. G., Sen, O., Baldini, E., Conti, L., Wietzycoski, C., Lopes, E., Pintar, T., Salobir, J., Aydin, C., Atici, S. D., Ergin, A., Ciyiltepe, H., Bozkurt, M. A., Kizilkaya, M. C., Onalan, N. B. D., Zuber, M. N. B. A., Wong, W. J., Garcia, A., Vidal, L., Beisani, M., Pasquier, J., Vilallonga, R., Sharma, S., Parmar, C., Lee, L., Sufi, P., Sinan, H., Saydam, M., İstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Sumer, Aziz, Peksen, Caghan, and Savas, Osman Anil
Subjects: Nutrition and Dietetics, Endocrinology, Diabetes and Metabolism, Gastric Bypass, Medicine (miscellaneous), nutritional and metabolic diseases, COVID-19, Gastrectomy, Humans, Morbidity, Propensity Score, Retrospective Studies, Treatment Outcome, Diabetes Mellitus, Type 2, Obesity, Morbid, Article, Diabetes Mellitus, Obesity, Morbid, Type 2
Abstract: Background There is a paucity of data comparing 30-day morbidity and mortality of sleeve gastrectomy (SG), Roux-en-Y gastric bypass (RYGB), and one anastomosis gastric bypass (OAGB). This study aimed to compare the 30-day safety of SG, RYGB, and OAGB in propensity score-matched cohorts. Materials and methods This analysis utilised data collected from the GENEVA study which was a multicentre observational cohort study of bariatric and metabolic surgery (BMS) in 185 centres across 42 countries between 01/05/2022 and 31/10/2020 during the Coronavirus Disease-2019 (COVID-19) pandemic. 30-day complications were categorised according to the Clavien–Dindo classification. Patients receiving SG, RYGB, or OAGB were propensity-matched according to baseline characteristics and 30-day complications were compared between groups. Results In total, 6770 patients (SG 3983; OAGB 702; RYGB 2085) were included in this analysis. Prior to matching, RYGB was associated with highest 30-day complication rate (SG 5.8%; OAGB 7.5%; RYGB 8.0% (p = 0.006)). On multivariate regression modelling, Insulin-dependent type 2 diabetes mellitus and hypercholesterolaemia were associated with increased 30-day complications. Being a non-smoker was associated with reduced complication rates. When compared to SG as a reference category, RYGB, but not OAGB, was associated with an increased rate of 30-day complications. A total of 702 pairs of SG and OAGB were propensity score-matched. The complication rate in the SG group was 7.3% (n = 51) as compared to 7.5% (n = 53) in the OAGB group (p = 0.68). Similarly, 2085 pairs of SG and RYGB were propensity score-matched. The complication rate in the SG group was 6.1% (n = 127) as compared to 7.9% (n = 166) in the RYGB group (p = 0.09). And, 702 pairs of OAGB and RYGB were matched. The complication rate in both groups was the same at 7.5 % (n = 53; p = 0.07). Conclusions This global study found no significant difference in the 30-day morbidity and mortality of SG, RYGB, and OAGB in propensity score-matched cohorts.
Published: 2021

2. P-287 Real-world data of perioperative FLOT in resectable gastric and gastro-esophagic junction cancer: The experience of a Portuguese institution

Author: Vilas-Boas, M., primary, Pina, M., additional, Coelho, S., additional, Cruz, A., additional, Silva, M., additional, Rosinha, A., additional, Carneiro, F., additional, Marques, D., additional, Faustino, C., additional, Sousa, N., additional, Ferreira, P., additional, and Fragoso, M., additional
Published: 2022
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3. 30-Day morbidity and mortality of bariatric metabolic surgery in adolescence during the COVID-19 pandemic – The GENEVA study

Author: Singhal R., Wiggins T., Super J., Alqahtani A., Nadler E. P., Ludwig C., Tahrani A., Mahawar K., Pedziwiatr M., Major P., Zarzycki P., Pantelis A., Lapatsanis D. P., Stravodimos G., Matthys C., Focquet M., Vleeschouwers W., Spaventa A. G., Zerrweck C., Vitiello A., Berardi G., Musella M., Sanchez-Meza A., Cantu F. J., Mora F., Cantu M. A., Katakwar A., Reddy D. N., Elmaleh H., Hassan M., Elghandour A., Elbanna M., Osman A., Khan A., Layani L., Kiran N., Velikorechin A., Solovyeva M., Melali H., Shahabi S., Agrawal A., Shrivastava A., Sharma A., Narwaria B., Narwaria M., Raziel A., Sakran N., Susmallian S., Karagoz L., Akbaba M., Piskin S. Z., Ziya A., Senol Z., Manno E., Iovino M. G., Qassem M., Arana-Garza S., Povoas H. P., Vilas-Boas M. L., Naumann D., Li A., Ammori B. J., Balamoun H., Salman M., Nasta A. M., Goel R., Sanchez-Aguilar H., Herrera M. F., Abou-Mrad A., Cloix L., Mazzini G. S., Kristem L., Lazaro A., Campos J., Bernardo J., Gonzalez J., Trindade C., Viveiros O., Ribeiro R., Goitein D., Hazzan D., Segev L., Beck T., Reyes H., Monterrubio J., Garcia P., Benois M., Kassir R., Contine A., Elshafei M., Aktas S., Weiner S., Heidsieck T., Level L., Pinango S., Ortega P. M., Moncada R., Valenti V., Vlahovic I., Boras Z., Liagre A., Martini F., Juglard G., Motwani M., Saggu S. S., Al Momani H., Lopez L. A. A., Cortez M. A. C., Zavala R. A., D'Haese C., Kempeneers I., Himpens J., Lazzati A., Paolino L., Bathaei S., Bedirli A., Yavuz A., Buyukkasap C., Ozaydin S., Kwiatkowski A., Bartosiak K., Waledziak M., Santonicola A., Angrisani L., Iovino P., Palma R., Iossa A., Boru C. E., De Angelis F., Silecchia G., Hussain A., Balchandra S., Coltell I. B., Perez J. L., Bohra A., Awan A. K., Madhok B., Leeder P. C., Awad S., Al-Khyatt W., Shoma A., Elghadban H., Ghareeb S., Mathews B., Kurian M., Larentzakis A., Vrakopoulou G. Z., Albanopoulos K., Bozdag A., Lale A., Kirkil C., Dincer M., Bashir A., Haddad A., Hijleh L. A., Zilberstein B., de Marchi D. D., Souza W. P., Broden C. M., Gislason H., Shah K., Ambrosi A., Pavone G., Tartaglia N., Kona S. L. K., Kalyan K., Perez C. E. G., Botero M. A. F., Covic A., Timofte D., Maxim M., Faraj D., Tseng L., Liem R., Oren G., Dilektasli E., Yalcin I., AlMukhtar H., Al Hadad M., Mohan R., Arora N., Bedi D., Rives-Lange C., Chevallier J. -M., Poghosyan T., Sebbag H., Zinai L., Khaldi S., Mauchien C., Mazza D., Dinescu G., Rea B., Perez-Galaz F., Zavala L., Besa A., Curell A., Balibrea J. M., Vaz C., Galindo L., Silva N., Caballero J. L. E., Sebastian S. O., Marchesini J. C. D., da Fonseca Pereira R. A., Sobottka W. H., Fiolo F. E., Turchi M., Coelho A. C. J., Zacaron A. L., Barbosa A., Quinino R., Menaldi G., Paleari N., Martinez-Duartez P., Aragon Ramirez de Esparza D. G. M., Esteban V. S., Torres A., Garcia-Galocha J. L., Josa M. I., Pacheco-Garcia J. M., Mayo-Ossorio M. A., Chowbey P., Soni V., de Vasconcelos Cunha H. A., Castilho M. V., Ferreira R. M. A., Barreiro T. A., Charalabopoulos A., Sdralis E., Davakis S., Bomans B., Dapri G., Van Belle K., MazenTakieddine, Vaneukem P., Karaca E. S. A., Karaca F. C., Sumer A., Peksen C., Savas O. A., Chousleb E., Elmokayed F., Fakhereldin I., Aboshanab H. M., Swelium T., Gudal A., Gamloo L., Ugale A., Ugale S., Boeker C., Reetz C., Hakami I. A., Mall J., Alexandrou A., Baili E., Bodnar Z., Maleckas A., Gudaityte R., Guldogan C. E., Gundogdu E., Ozmen M. M., Thakkar D., Dukkipati N., Shah P. S., Shah S. S., Adil M. T., Jambulingam P., Mamidanna R., Whitelaw D., Jain V., Veetil D. K., Wadhawan R., Torres M., Tinoco T., Leclercq W., Romeijn M., van de Pas K., Alkhazraji A. K., Taha S. A., Ustun M., Yigit T., Inam A., Burhanulhaq M., Pazouki A., Eghbali F., Kermansaravi M., Jazi A. H. D., Mahmoudieh M., Mogharehabed N., Tsiotos G., Stamou K., Barrera Rodriguez F. J., Rojas Navarro M. A., Torres O. M. O., Martinez S. L., Tamez E. R. M., Millan Cornejo G. A., Flores J. E. G., Mohammed D. A., Elfawal M. H., Shabbir A., Guowei K., So J. B. Y., Kaplan E. T., Kaplan M., Kaplan T., Pham D. T., Rana G., Kappus M., Gadani R., Kahitan M., Pokharel K., Osborne A., Pournaras D., Hewes J., Napolitano E., Chiappetta S., Bottino V., Dorado E., Schoettler A., Gaertner D., Fedtke K., Aguilar-Espinosa F., Aceves-Lozano S., Balani A., Nagliati C., Pennisi D., Rizzi A., Frattini F., Foschi D., Benuzzi L., Parikh C. H. I. R. A. G., Shah H. A. R. S. H. I. L., Pinotti E., Montuori M., Borrelli V., Dargent J., Copaescu C. A., Hutopila I., Smeu B., Witteman B., Hazebroek E., Deden L., Heusschen L., Okkema S., Aufenacker T., den Hengst W., Vening W., van der Burgh Y., Ghazal A., Ibrahim H., Niazi M., Alkhaffaf B., Altarawni M., Cesana G. C., Anselmino M., Uccelli M., Olmi S., Stier C., Akmanlar T., Sonnenberg T., Schieferbein U., Marcolini A., Awruch D., Vicentin M., de Souza Bastos E. L., Gregorio S. A., Ahuja A., Mittal T., Bolckmans R., Baratte C., Wisnewsky J. A., Genser L., Chong L., Taylor L., Ward S., Hi M. W., Heneghan H., Fearon N., Plamper A., Rheinwalt K., Geoghegan J., Ng K. C., Kaseja K., Kotowski M., Samarkandy T. A., Leyva-Alvizo A., Corzo-Culebro L., Wang C., Yang W., Dong Z., Riera M., Jain R., Hamed H., Said M., Zarzar K., Garcia M., Turkcapar A. G., Sen O., Baldini E., Conti L., Wietzycoski C., Lopes E., Pintar T., Salobir J., Aydin C., Atici S. D., Ergin A., Ciyiltepe H., Bozkurt M. A., Kizilkaya M. C., Onalan N. B. D., Zuber M. N. B. A., Wong W. J., Garcia A., Vidal L., Beisani M., Pasquier J., Vilallonga R., Sharma S., Parmar C., Lee L., Sufi P., Sinan H., Saydam M., Singhal, R., Wiggins, T., Super, J., Alqahtani, A., Nadler, E. P., Ludwig, C., Tahrani, A., Mahawar, K., Pedziwiatr, M., Major, P., Zarzycki, P., Pantelis, A., Lapatsanis, D. P., Stravodimos, G., Matthys, C., Focquet, M., Vleeschouwers, W., Spaventa, A. G., Zerrweck, C., Vitiello, A., Berardi, G., Musella, M., Sanchez-Meza, A., Cantu, F. J., Mora, F., Cantu, M. A., Katakwar, A., Reddy, D. N., Elmaleh, H., Hassan, M., Elghandour, A., Elbanna, M., Osman, A., Khan, A., Layani, L., Kiran, N., Velikorechin, A., Solovyeva, M., Melali, H., Shahabi, S., Agrawal, A., Shrivastava, A., Sharma, A., Narwaria, B., Narwaria, M., Raziel, A., Sakran, N., Susmallian, S., Karagoz, L., Akbaba, M., Piskin, S. Z., Ziya, A., Senol, Z., Manno, E., Iovino, M. G., Qassem, M., Arana-Garza, S., Povoas, H. P., Vilas-Boas, M. L., Naumann, D., Li, A., Ammori, B. J., Balamoun, H., Salman, M., Nasta, A. M., Goel, R., Sanchez-Aguilar, H., Herrera, M. F., Abou-Mrad, A., Cloix, L., Mazzini, G. S., Kristem, L., Lazaro, A., Campos, J., Bernardo, J., Gonzalez, J., Trindade, C., Viveiros, O., Ribeiro, R., Goitein, D., Hazzan, D., Segev, L., Beck, T., Reyes, H., Monterrubio, J., Garcia, P., Benois, M., Kassir, R., Contine, A., Elshafei, M., Aktas, S., Weiner, S., Heidsieck, T., Level, L., Pinango, S., Ortega, P. M., Moncada, R., Valenti, V., Vlahovic, I., Boras, Z., Liagre, A., Martini, F., Juglard, G., Motwani, M., Saggu, S. S., Al Momani, H., Lopez, L. A. A., Cortez, M. A. C., Zavala, R. A., D'Haese, C., Kempeneers, I., Himpens, J., Lazzati, A., Paolino, L., Bathaei, S., Bedirli, A., Yavuz, A., Buyukkasap, C., Ozaydin, S., Kwiatkowski, A., Bartosiak, K., Waledziak, M., Santonicola, A., Angrisani, L., Iovino, P., Palma, R., Iossa, A., Boru, C. E., De Angelis, F., Silecchia, G., Hussain, A., Balchandra, S., Coltell, I. B., Perez, J. L., Bohra, A., Awan, A. K., Madhok, B., Leeder, P. C., Awad, S., Al-Khyatt, W., Shoma, A., Elghadban, H., Ghareeb, S., Mathews, B., Kurian, M., Larentzakis, A., Vrakopoulou, G. Z., Albanopoulos, K., Bozdag, A., Lale, A., Kirkil, C., Dincer, M., Bashir, A., Haddad, A., Hijleh, L. A., Zilberstein, B., de Marchi, D. D., Souza, W. P., Broden, C. M., Gislason, H., Shah, K., Ambrosi, A., Pavone, G., Tartaglia, N., Kona, S. L. K., Kalyan, K., Perez, C. E. G., Botero, M. A. F., Covic, A., Timofte, D., Maxim, M., Faraj, D., Tseng, L., Liem, R., Oren, G., Dilektasli, E., Yalcin, I., Almukhtar, H., Al Hadad, M., Mohan, R., Arora, N., Bedi, D., Rives-Lange, C., Chevallier, J. -M., Poghosyan, T., Sebbag, H., Zinai, L., Khaldi, S., Mauchien, C., Mazza, D., Dinescu, G., Rea, B., Perez-Galaz, F., Zavala, L., Besa, A., Curell, A., Balibrea, J. M., Vaz, C., Galindo, L., Silva, N., Caballero, J. L. E., Sebastian, S. O., Marchesini, J. C. D., da Fonseca Pereira, R. A., Sobottka, W. H., Fiolo, F. E., Turchi, M., Coelho, A. C. J., Zacaron, A. L., Barbosa, A., Quinino, R., Menaldi, G., Paleari, N., Martinez-Duartez, P., Aragon Ramirez de Esparza, D. G. M., Esteban, V. S., Torres, A., Garcia-Galocha, J. L., Josa, M. I., Pacheco-Garcia, J. M., Mayo-Ossorio, M. A., Chowbey, P., Soni, V., de Vasconcelos Cunha, H. A., Castilho, M. V., Ferreira, R. M. A., Barreiro, T. A., Charalabopoulos, A., Sdralis, E., Davakis, S., Bomans, B., Dapri, G., Van Belle, K., Mazentakieddine, Vaneukem, P., Karaca, E. S. A., Karaca, F. C., Sumer, A., Peksen, C., Savas, O. A., Chousleb, E., Elmokayed, F., Fakhereldin, I., Aboshanab, H. M., Swelium, T., Gudal, A., Gamloo, L., Ugale, A., Ugale, S., Boeker, C., Reetz, C., Hakami, I. A., Mall, J., Alexandrou, A., Baili, E., Bodnar, Z., Maleckas, A., Gudaityte, R., Guldogan, C. E., Gundogdu, E., Ozmen, M. M., Thakkar, D., Dukkipati, N., Shah, P. S., Shah, S. S., Adil, M. T., Jambulingam, P., Mamidanna, R., Whitelaw, D., Jain, V., Veetil, D. K., Wadhawan, R., Torres, M., Tinoco, T., Leclercq, W., Romeijn, M., van de Pas, K., Alkhazraji, A. K., Taha, S. A., Ustun, M., Yigit, T., Inam, A., Burhanulhaq, M., Pazouki, A., Eghbali, F., Kermansaravi, M., Jazi, A. H. D., Mahmoudieh, M., Mogharehabed, N., Tsiotos, G., Stamou, K., Barrera Rodriguez, F. J., Rojas Navarro, M. A., Torres, O. M. O., Martinez, S. L., Tamez, E. R. M., Millan Cornejo, G. A., Flores, J. E. G., Mohammed, D. A., Elfawal, M. H., Shabbir, A., Guowei, K., So, J. B. Y., Kaplan, E. T., Kaplan, M., Kaplan, T., Pham, D. T., Rana, G., Kappus, M., Gadani, R., Kahitan, M., Pokharel, K., Osborne, A., Pournaras, D., Hewes, J., Napolitano, E., Chiappetta, S., Bottino, V., Dorado, E., Schoettler, A., Gaertner, D., Fedtke, K., Aguilar-Espinosa, F., Aceves-Lozano, S., Balani, A., Nagliati, C., Pennisi, D., Rizzi, A., Frattini, F., Foschi, D., Benuzzi, L., Parikh, C. H. I. R. A. G., Shah, H. A. R. S. H. I. L., Pinotti, E., Montuori, M., Borrelli, V., Dargent, J., Copaescu, C. A., Hutopila, I., Smeu, B., Witteman, B., Hazebroek, E., Deden, L., Heusschen, L., Okkema, S., Aufenacker, T., den Hengst, W., Vening, W., van der Burgh, Y., Ghazal, A., Ibrahim, H., Niazi, M., Alkhaffaf, B., Altarawni, M., Cesana, G. C., Anselmino, M., Uccelli, M., Olmi, S., Stier, C., Akmanlar, T., Sonnenberg, T., Schieferbein, U., Marcolini, A., Awruch, D., Vicentin, M., de Souza Bastos, E. L., Gregorio, S. A., Ahuja, A., Mittal, T., Bolckmans, R., Baratte, C., Wisnewsky, J. A., Genser, L., Chong, L., Taylor, L., Ward, S., Hi, M. W., Heneghan, H., Fearon, N., Plamper, A., Rheinwalt, K., Geoghegan, J., Ng, K. C., Kaseja, K., Kotowski, M., Samarkandy, T. A., Leyva-Alvizo, A., Corzo-Culebro, L., Wang, C., Yang, W., Dong, Z., Riera, M., Jain, R., Hamed, H., Said, M., Zarzar, K., Garcia, M., Turkcapar, A. G., Sen, O., Baldini, E., Conti, L., Wietzycoski, C., Lopes, E., Pintar, T., Salobir, J., Aydin, C., Atici, S. D., Ergin, A., Ciyiltepe, H., Bozkurt, M. A., Kizilkaya, M. C., Onalan, N. B. D., Zuber, M. N. B. A., Wong, W. J., Garcia, A., Vidal, L., Beisani, M., Pasquier, J., Vilallonga, R., Sharma, S., Parmar, C., Lee, L., Sufi, P., Sinan, H., and Saydam, M.
Subjects: Male, Pediatrics, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Adolescent, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), bariatric surgery, Context (language use), Pandemic, Medicine, Humans, Pandemics, COVID-19, pandemic, SARS-CoV-2, Nutrition and Dietetics, Manchester Cancer Research Centre, business.industry, Health Policy, ResearchInstitutes_Networks_Beacons/mcrc, Public Health, Environmental and Occupational Health, medicine.disease, Obesity, Obesity, Morbid, Treatment Outcome, Pediatrics, Perinatology and Child Health, Cohort, Female, Morbidity, business, Body mass index, Cohort study, Human
Abstract: Background: Metabolic and bariatric surgery (MBS) is an effective treatment for adolescents with severe obesity. Objectives: This study examined the safety of MBS in adolescents during the coronavirus disease 2019 (COVID-19) pandemic. Methods: This was a global, multicentre and observational cohort study of MBS performed between May 01, 2020, and October 10,2020, in 68 centres from 24 countries. Data collection included in-hospital and 30-day COVID-19 and surgery-specific morbidity/mortality. Results: One hundred and seventy adolescent patients (mean age: 17.75 ± 1.30 years), mostly females (n=122, 71.8%), underwent MBS during the study period. The mean pre-operative weight and body mass index were 122.16 ± 15.92 kg and 43.7± 7.11 kg/m2, respectively. Although majority of patients had pre-operative testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (n=146; 85.9%), only 42.4% (n=72) of the patients were asked to self-isolate pre-operatively. Two patients developed symptomatic SARS-CoV-2 infection post-operatively (1.2%). The overall complication rate was 5.3% (n=9). There was no mortality in this cohort. Conclusions: MBS in adolescents with obesity is safe during the COVID-19 pandemic when performed within the context of local precautionary procedures (such as pre-operative testing). The 30-day morbidity rates were similar to those reported pre-pandemic. These data will help facilitate the safe re-introduction of MBS services for this group of patients.
Published: 2021

4. Electrochemical characterization of a novel salen-type modified electrode

Author: Vilas-Boas, M., Freire, C., Castro, B. de, and Hillman, A.R.
Subjects: Electrodes, Ion selective -- Analysis, Nickel -- Analysis, Complex compounds -- Analysis, Oxidation-reduction reaction -- Analysis, Chemicals, plastics and rubber industries
Abstract: The electrochemical properties of nickel(II) complexes with Schiff base ligands have been studied. Polymer films have been prepared by oxidatively electropolymerizing the nickel(II) complex on Pt electrodes in CH3CN/O.1 mol dm (super -3) tetraethylammonium perchlorate. Results indicate that films of poly(Ni(saltMe)) can be made to exhibit three types of charge transport modes by altering the film thickness and the experimental time scale. There is also a transition from semi-infinite diffusion to finite diffusion with time observing the potential step.
Published: 1998

5. The Volatile Composition of Portuguese Propolis Towards its Origin Discrimination

Author: Soraia I. Falcão, Freire, C., Cristina Figueiredo, A., and Vilas-Boas, M.
Subjects: Volatiles, GC, Populus x canadensis, Propolis, lcsh:QK1-989, lcsh:Chemistry, lcsh:QD241-441, volatiles, lcsh:QD1-999, lcsh:Organic chemistry, lcsh:Botany, Cistus ladanifer, GC-MS
Abstract: The volatiles from thirty six propolis samples collected from six different geographical locations in Portugal (mainland, Azores archipelago and Madeira Island) were evaluated. Populus x canadensis Moenchen leaf-buds and Cistus ladanifer L. branches essential oils were comparatively analysed. The essential oils were isolated by hydrodistillation and analysed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS). Cluster analysis based on propolis samples volatiles chemical composition defined three main clusters, not related to sample site collection. Cluster I grouped 28 samples with high relative amounts of oxygen-containing sesquiterpenes (20-77%), while cluster II grouped 7 samples rich in oxygen-containing monoterpenes (9-65%) and the only sample from cluster III was monoterpene hydrocarbons rich (26%). Although Populus x canadensis and Cistus ladanifer were associated as resin sources of Portuguese propolis, other Populus species as well as plants like Juniperus genus may contribute to the resin in specific geographical locations. Soraia I. Falcão thanks Fundação para a Ciência e a Tecnologia (FCT) for the PhD grant SFRH/BD/44855/2008, financially supported by POPH-QREN and FSE. This study was partially funded by FCT, under Pest-OE/EQB/LA0023/2011 and PTDC/CVT-EPI/2473/2012. Thanks to National Federation of Portuguese Beekeepers for propolis samples. info:eu-repo/semantics/publishedVersion
Published: 2016

6. A Collaborative Study for Performance Evaluation of Analytical Methods for Propolis. An IHC Trial

Author: LOPES, M., PEREYRA, A., KUNERT, C., BECKH, G., SCHREITER, H., ÇELEMLI, O. G., SORKUN, K., GEORGÉ, S, PAULO, L., GARDINI, S., SANCHO, M.t, BANKOVA, V., DASTAN, T., TANANAKI, C., NUNES, L.f., and VILAS-BOAS, M.
Subjects: Propolis
Abstract: Propolis is well known for its potential applications but also for its chemical complexity, making it very hard to define quality standards for its commercialization. Every day it is possible to find new publications on this bee product describing a new singularity, a particular substance with high bioactivity never described or a new application. This systematic increment in scientific information, although relevant, do not always enable a clear comparison between samples due to the different methods and procedures used to assess its quality parameters. To strength the scientific studies on propolis and establish a background for future definition of standards for industry, producers and laboratories, the Propolis Working group of the International Honey Commission, performed an inter-laboratory study to harmonize basic analytical methods and evaluate their accuracy and robustness. The collaborative study was accomplished by 12 laboratories from 9 countries and a company experienced in propolis trade. The samples under analysis, representing fifteen different origins around the globe, where fractionated and distributed to each laboratory, and so all participants analysed the same material. Overall, six parameters were evaluated: ashes, wax and balsamic extract on raw propolis, and total-phenolics, flavones and flavanones on propolis extract. The results were analysed following the international guidelines ISO 57251. Outliers were removed using Cochran’s test, to check the homogeneity of variances at certain levels, and Grubb test’s, to check the consistency of the laboratories average. The results revealed good reproducibility for ash, wax, balsamic content and flavones, while for flavanones the method is clearly unsuitable. Acknowledgements: Project PDR2020-1.0.1-FEADER-031734: “Diversification and Innovation on Beekeeping Production”
Published: 2018

7. Skin temperature of the foot: A comparative study between familial amyloid polyneuropathy and diabetic foot patients

Author: Seixas,A, do Carmo Vilas Boas,M, Carvalho,R, Coelho,T, Ammer,K, Vilas Boas,JP, Vardasca,R, João Paulo Cunha, and Mendes,J
Published: 2018

8. P278Validation of HMPAO-Tc-99m as a myocardial perfusion radiotracer: preliminary results

Author: Faria, M T, primary, Oliveira, A, additional, Vilas-Boas, M C, additional, Maia, P M, additional, Rego, R, additional, Sousa, J, additional, Pereira, J, additional, Cunha, J P S, additional, Rocha-Goncalves, F, additional, and Martins, E, additional
Published: 2019
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9. Factors predictive of outcome before and after transplantation for fulminant hepatic failure

Author: Rocha-Filho, J., primary, Figueira, E., additional, Lanchotte, C., additional, Assalin, A., additional, Romano, V., additional, Shinkado, Y., additional, Vilas-Boas, M., additional, Ferreira, L., additional, Galvao, F., additional, Chaib, E., additional, and D'Albuquerque, L., additional
Published: 2017
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10. Older age did not increase short-term surgical complications after laparoscopic cholecystectomy

Author: Figueira, E., primary, Rocha-Filho, J., additional, Bacchella, T., additional, Costa, T., additional, Vilas-Boas, M., additional, Romano, V., additional, Casagrande, S., additional, Sacramento, I., additional, and Cecconello, I., additional
Published: 2017
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11. Application of GIS in potential beekeeping assessment: Case study of Montesinho Natural Park (Portugal)

Author: Vilas-Boas, M., Frenandez, P., Garção, H., Roque, N., Peres, M.F., Gonçalves, M., and Anjos, O.
Subjects: Beekeeping assessment, GIS
Abstract: Planners require solutions that address routine work needs and seems essential to improving efficiency and productivity. There are a great number of different factors related to beekeeper activity as well the quality and productivity of different bee products. The spatial analysis is a powerful tool for overlap and relates various levels of information on a map, and consequently a very useful for beekeeping activity planning. This work proposes and applies a methodology to potential beekeeping assessment in Montesinho Natural Park, a region in the northwest of Portugal. The beekeeping potential maps were developed with the following data sources: legal standards, vegetation, land use, topography, water resources, roads, electromagnetic fields, and some honey physico-chemical analysis. The design and implementation of spatial analysis model based on Geographic Information System (GIS) to beekeeping planning activities has already been described by Anjos et al (2014). Spatial analysis techniques allows to define the potential beekeeper map supporting the beekeeper management in this region. Anjos O, Silva G, Roque N, Fernandez P, 2014. GIS based analysis to support the beekeeping planning. Book of abstracts of the International Symposium on Bee Products 3rd edition – Annual meeting of the International Honey Commission (IHC), Faculty of medicine, University of Rijeka, p:61
Published: 2015

12. Hipotecando o futuro : o caso das doenças sexualmente transmissíveis no noroeste português

Author: Paula Remoaldo, Vilas Boas, M. A., Sousa, P. Barbosa, Santos, J. Marinho, and Universidade do Minho
Subjects: Doenças sexualmente transmissíveis, Comportamentos, Sexualidade, Geografia da saúde, Behaviours, Educação para a saúde, Sexuality, Health Education, Sexually Transmissible Diseases, Health Geography
Abstract: O presente artigo aborda a temática das Doenças Sexualmente Transmisíveis relacionando-se com os resultados inerentes a um projecto de investigação desenvolvido no Departamento de Geografia da Universidade do Minho durante o período de Março de 2002 a Março de 2003 e financiado pela Fundação Calouste Gulbenkian, Este projecto teve como objectivos principais averiguar o conhecimento e comportamentos dos alunos da Universidade do Minho (Noroeste de Portugal) relativamente a este tipo de doenças, aferir quais são as acções de prevenção que têm sido encetadas em Portugal até ao momento, elaborar e distribuir um panfleto informativo e criar um site informativo na Internet. Neste artigo apresentamos os resultados mais relevantes do inquérito por questionário auto-administrado aplicado a 677 alunos daquela Universidade, tendo sido diagnosticado um baixo conhecimento das características e implicações das doenças analisadas., This article approaches the subject of the Sexually Transmissible Diseases pursuant to the results of a research project of investigation developed in the Department of Geography from Minho University during the period from March of 2002 to March of 2003 and financed by the Calouste Gulbenkian Foundation. The principal goals of this project was to understand the knowledge and the behaviours of the students from the Minho University (Northwest of Portugal) related to this kind of diseases, to find out the preventive actions used in Portugal up to this moment, to create and distribute informative leaflets and to create informative internet site. In this article we present the most important results obtained by a self-administrated inquiry through the use of a questionnaire given to 677 students from that University. The conclusion of this questionnaire was that the students had a low knowledge of the characteristics and implications of the analysed diseases.
Published: 2005

13. P278 Validation of HMPAO-Tc-99m as a myocardial perfusion radiotracer: preliminary results.

Author: Faria, M T, Oliveira, A, Vilas-Boas, M C, Maia, P M, Rego, R, Sousa, J, Pereira, J, Cunha, J P S, Rocha-Goncalves, F, and Martins, E
Subjects: CARDIOMYOPATHIES, CONFERENCES & conventions, PERFUSION, RADIONUCLIDE imaging, RADIOISOTOPES, SINGLE-photon emission computed tomography, PREVENTION
Published: 2019
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14. Information architecture for intelligent decision support in intensive medicine

Author: Santos, M. F., Portela, F., Vilas-Boas, M., Machado, J., Abelha, A., Neves, J., Álvaro Silva, Rua, F., and Universidade do Minho
Subjects: Real-time data acquisition, Intensive care, INTCare, Information models, Knowledge discovery in databases, Intelligent decision support systems
Abstract: Daily, a great amount of data that is gathered in intensive care units, which makes intensive medicine a very attractive field for applying knowledge discovery in databases. Previously unknown knowledge can be extracted from that data in order to create prediction and decision models. The challenge is to perform those tasks in real-time, in order to assist the doctors in the decision making process. Furthermore, the models should be continuously assessed and optimized, if necessary, to maintain a certain accuracy. In this paper we propose an information architecture to support an adjustment to the INTCare system, an intelligent decision support system for intensive medicine. We focus on the automatization of data acquisition avoiding human intervention, describing its steps and some requirements., (undefined)

15. INTCARE : multi-agent approach for real-time intelligent decision support in intensive medicine

Author: Santos, M. F., Filipe Portela, Vilas-Boas, M., and Universidade do Minho
Subjects: Artificial intelligence, Science & Technology, Agent intelligent Decision support systems, Intensive care, Multi-agent systems, Intelligent decision support Systems, Agent
Abstract: For an Intelligent Decision Support System to work in real-time, it is of great value the use of intelligent agents that cooperate with each other to accomplish their tasks. In a critical environment like an Intensive Care Unit, doctors should have the right information, at the right time, to better assist their patients. In this paper we present an architecture for a Multi-Agents System that will support doctors’ decision by in real-time, guaranteeing that all required clinical data is available and capable of predicting the patients’ condition for the next hour., Fundação para a Ciência e a Tecnologia (FCT)

16. Bee Venom-Loaded Niosomes as Innovative Platforms for Cancer Treatment: Development and Therapeutical Efficacy and Safety Evaluation.

Author: Pinto MB, Pires PC, Calhelha RC, Silva AR, Sousa MJ, Vilas-Boas M, Falcão SI, Veiga F, Makvandi P, and Paiva-Santos AC
Abstract: Despite past efforts towards therapeutical innovation, cancer remains a highly incident and lethal disease, with current treatments lacking efficiency and leading to severe side effects. Hence, it is imperative to develop new, more efficient, and safer therapies. Bee venom has proven to have multiple and synergistic bioactivities, including antitumor effects. Nevertheless, some toxic effects have been associated with its administration. To tackle these issues, in this work, bee venom-loaded niosomes were developed, for cancer treatment. The vesicles had a small (150 nm) and homogeneous (polydispersity index of 0.162) particle size, and revealed good therapeutic efficacy in in vitro gastric, colorectal, breast, lung, and cervical cancer models (inhibitory concentrations between 12.37 ng/mL and 14.72 ng/mL). Additionally, they also revealed substantial anti-inflammatory activity (inhibitory concentration of 28.98 ng/mL), effects complementary to direct antitumor activity. Niosome safety was also assessed, both in vitro (skin, liver, and kidney cells) and ex vivo (hen's egg chorioallantoic membrane), and results showed that compound encapsulation increased its safety. Hence, small, and homogeneous bee venom-loaded niosomes were successfully developed, with substantial anticancer and anti-inflammatory effects, making them potentially promising primary or adjuvant cancer therapies. Future research should focus on evaluating the potential of the developed platform in in vivo models., Competing Interests: The authors declare no conflicts of interest.
Published: 2024
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17. Using Analytic Hierarchy Process to Assess Beekeeping Suitability in Portuguese Controlled Areas: A First Approach.

Author: Roque N, Fernandez P, Silveira C, Vilas-Boas M, and Anjos O
Abstract: Beekeeping management is greatly influenced by spatial factors (e.g., land use/land cover, roads, or electrical energy networks), so GIS are a powerful tool to overlap and relate a variety of spatial data levels and, consequently, a very useful tool for beekeeping activity planning. This study was developed within the intervention area of three controlled zones managed by Portuguese Beekeepers Associations. The methodology, based on multi-criteria decision analysis, integrates several criteria, such as hydrographic networks, road networks, soil occupation, solar radiation, and electromagnetic radiation sources. These criteria were proposed and evaluated through online questionnaires carried out with beekeepers. Concerning the selected criteria and the respective geographical data, the most relevant were land use/land cover and water availability, with a significance of 44% and 24%, respectively. The beekeeping suitability map enabled us to evaluate the degree of compliance for the actual location of apiaries, with 60% of the apiaries being installed in high potential areas. In the context of beekeeping planning, the potential of the techniques applied seems to be an important tool for optimizing the location of apiaries and the profitability of beekeeping.
Published: 2024
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18. Differentiating between Monofloral Portuguese Bee Pollens Using Phenolic and Volatile Profiles and Their Impact on Bioactive Properties.

Author: Larbi S, Aylanc V, Rodríguez-Flores MS, Calhelha RC, Barros L, Rezouga F, Seijo MC, Falcão SI, and Vilas-Boas M
Subjects: Bees, Animals, Portugal, Flavonoids, Pollen chemistry, Phenols analysis, Antioxidants pharmacology
Abstract: Nowadays, bee products are commended by consumers for their medicinal and dietary properties. This study aimed to differentiate between monofloral bee pollens originating from Portugal using phenolic and volatile profiles and investigate their antioxidant and cytotoxic activity. Total phenolic and flavonoid compounds were recorded between 2.9-35.8 mg GAE/g and 0.7-4.8 mg QE/g, respectively. The LC/DAD/ESI-MS n analytical results allowed us to identify and quantify a total of 72 compounds, including phenolic and phenylamide compounds, whereas GC-MS results revealed the presence of 49 different compounds, mostly ketones, aldehydes, esters, hydrocarbons, and terpenes. The highest DPPH • radical scavenging activity, EC 50 : 0.07 mg/mL, was recorded in the sample dominated by Castanae sp. pollen, whereas the Rubus sp. (1.59 mM Trolox/mg) and Cistaceae sp. (0.09 mg GAE/g) pollen species exhibited the highest antioxidant activity in ABTS •+ and reducing power assays, respectively. Regarding the anti-carcinogenic activity, only Carduus sp. showed remarkable cytotoxic potential against MCF-7.
Published: 2023
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19. Development and Characterization of High-Absorption Microencapsulated Organic Propolis EPP-AF ® Extract (i-CAPs).

Author: Berretta AA, De Lima JA, Falcão SI, Calhelha R, Amorim NA, Gonçalves IS, Zamarrenho LG, Barud HDS, Bastos JK, De Jong D, and Vilas-Boas M
Subjects: Humans, Antioxidants pharmacology, Antioxidants analysis, Capsules, Spectroscopy, Fourier Transform Infrared, Caco-2 Cells, Ethanol, Water, Anti-Inflammatory Agents pharmacology, Propolis pharmacology, Anti-Infective Agents pharmacology
Abstract: The demand for organic and functional food continues to increase yearly. Among the available functional foods, propolis is a bee product that has various beneficial properties, including antimicrobial, antioxidant, and anti-inflammatory activities. However, it generally is only available in ethanol solution, which has poor bioavailability, as it is relatively insoluble in water. The use of such ethanol extracts is often objectionable because of the alcohol content and because they have a strong and striking taste. Development of alternatives that can efficiently and safely increase solubility in water, and that meet organic production specifications, has been a challenge. To address these concerns, microcapsules were developed using spray-dryer technology from an emulsion based on EPP-AF ® propolis and gum arabic (i-CAPS). These propolis-loaded microcapsules were characterized using FT-IR, SEM, TGA, HPLC, and spectrophotometric techniques, along with determination of antimicrobial, antioxidant, antitumor, anti-inflammatory, and antihypercholesterolemic activities, as well as permeability in in vitro models. The production system resulted in microcapsules with a spherical shape and an encapsulation efficiency of 93.7 ± 0.7%. They had IC50s of 2.654 ± 0.062 and 7.342 ± 0.058 µg/mL by FRAP and DPPH antioxidant methods, respectively. The EPP-AF ® i-CAPS also had superior antimicrobial activity against Gram-positive bacteria. Antitumor activity was calculated based on the concentration that inhibited 50% of growth of AGS, Caco-2, and MCF-7 cell strains, giving results of 154.0 ± 1.0, 117 ± 1.0, and 271.0 ± 25 µg/mL, respectively. The microcapsule presentation reduced the permeation of cholesterol by 53.7%, demonstrating antihypercholesterolemic activity, and it improved the permeability of p -coumaric acid and artepillin C. The IC50 for NO production in RAW 264.7 cells was 59.0 ± 0.1 µg/mL. These findings demonstrate the potential of this new propolis product as a food and pharmaceutical ingredient, though additional studies are recommended to validate the safety of proposed dosages.
Published: 2023
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20. Thermal Stability and Antioxidant Activity of Bioactive Compounds in Bread Enriched with Bee Pollen and Bee Bread.

Author: Ertosun S, Aylanc V, Falcão SI, and Vilas-Boas M
Abstract: Bee pollen (BP) and bee bread (BB) are natural food sources containing a wide variety of bioactive compounds, complementing their rich nutritional composition. These bee products are being explored to empower functional foods, with the term functionality being dependent on the bioactive compounds added to the food matrix. However, there is not enough evidence of the effect of heat on these compounds during food processing and production and how it impacts their biological activity. Here, we enriched traditional bread by adding BP and BB at different proportions of 1 to 5% and tested the thermal stability of their bioactive compounds through several spectroscopic and chromatographic analyses. Adding bee pollen and bee bread to bread resulted in a 4 and 5-fold increase in total phenolic content, respectively. While not all the 38 phenolic and phenolamide compounds identified in the raw BP and BB were detected in the processed bread, phenolamides were found to be more resilient to baking and heat treatment than flavonoids. Still, the enriched bread's antioxidant activity improved with the addition of BP and BB. Therefore, incorporating bee products into heat-treated products could enhance the functionality of staple foods and increase the accessibility to these natural products.
Published: 2023
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21. Editorial: Therapeutic potential of propolis-from in vitro studies to clinical trials.

Author: Bobiş O, Berretta AA, Vilas-Boas M, and De Jong D
Abstract: Competing Interests: Author AB was employed by the company Apis Flora Indl Coml Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Published: 2023
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22. Beneficial Effect of Bee Venom and Its Major Components on Facial Nerve Injury Induced in Mice.

Author: Er-Rouassi H, Bakour M, Touzani S, Vilas-Boas M, Falcão S, Vidal C, and Lyoussi B
Subjects: Animals, Mice, Betamethasone, Melitten pharmacology, Melitten therapeutic use, Phospholipases A2, Bee Venoms pharmacology, Bee Venoms therapeutic use, Facial Nerve Injuries drug therapy
Abstract: Peripheral nerve injury (PNI) is a health problem that affects many people worldwide. This study is the first to evaluate the potential effect of bee venom (BV) and its major components in a model of PNI in the mouse. For that, the BV used in this study was analyzed using UHPLC. All animals underwent a distal section-suture of facial nerve branches, and they were randomly divided into five groups. Group 1: injured facial nerve branches without any treatment. Group 2: the facial nerve branches were injured, and the normal saline was injected similarly as in the BV-treated group. Group 3: injured facial nerve branches with local injections of BV solution. Group 4: injured facial nerve branches with local injections of a mixture of PLA2 and melittin. Group 5: injured facial nerve branches with local injection of betamethasone. The treatment was performed three times a week for 4 weeks. The animals were submitted to functional analysis (observation of whisker movement and quantification of nasal deviation). The vibrissae muscle re-innervation was evaluated by retrograde labeling of facial motoneurons in all experimental groups. UHPLC data showed 76.90 ± 0.13%, 11.73 ± 0.13%, and 2.01 ± 0.01%, respectively, for melittin, phospholipase A2, and apamin in the studied BV sample. The obtained results showed that BV treatment was more potent than the mixture of PLA2 and melittin or betamethasone in behavioral recovery. The whisker movement occurred faster in BV-treated mice than in the other groups, with a complete disappearance of nasal deviation two weeks after surgery. Morphologically, a normal fluorogold labeling of the facial motoneurons was restored 4 weeks after surgery in the BV-treated group, but no such restoration was ever observed in other groups. Our findings indicate the potential of the use of BV injections to enhance appropriate functional and neuronal outcomes after PNI.
Published: 2023
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23. Improvement of the In Vitro Cytotoxic Effect on HT-29 Colon Cancer Cells by Combining 5-Fluorouacil and Fluphenazine with Green, Red or Brown Propolis.

Author: Falcão SI, Duarte D, Diallo M, Santos J, Ribeiro E, Vale N, and Vilas-Boas M
Subjects: Humans, HT29 Cells, Fluphenazine, Fluorouracil pharmacology, Phenols pharmacology, Phenols chemistry, Propolis pharmacology, Propolis chemistry, Antineoplastic Agents pharmacology, Colonic Neoplasms drug therapy
Abstract: Cancer is regard as one of the key factors of mortality and morbidity in the world. Treatment is mainly based on chemotherapeutic drugs that, when used in targeted therapies, have serious side effects. 5-fluorouracil (5-FU) is a drug commonly used against colorectal cancer (CRC), despite its side effects. Combination of this compound with natural products is a promising source in cancer treatment research. In recent years, propolis has become the subject of intense pharmacological and chemical studies linked to its diverse biological properties. With a complex composition rich in phenolic compounds, propolis is described as showing positive or synergistic interactions with several chemotherapeutic drugs. The present work evaluated the in vitro cytotoxic activity of the most representative propolis types, such as green, red and brown propolis, in combination with chemotherapeutic or CNS drugs on HT-29 colon cancer cell lines. The phenolic composition of the propolis samples was evaluated by LC-DAD-ESI/MS n analysis. According to the type of propolis, the composition varied; green propolis was rich in terpenic phenolic acids and red propolis in polyprenylated benzophenones and isoflavonoids, while brown propolis was composed mainly of flavonoids and phenylpropanoids. Generally, for all propolis types, the results demonstrated that combing propolis with 5-FU and fluphenazine successfully enhances the in vitro cytotoxic activity. For green propolis, the combination demonstrated an enhancement of the in vitro cytotoxic effect compared to green propolis alone, at all concentrations, while for brown propolis, the combination in the concentration of 100 μg/mL gave a lower number of viable cells, even when compared with 5-FU or fluphenazine alone. The same was observed for the red propolis combination, but with a higher reduction in cell viability. The combination index, calculated based on the Chou-Talalay method, suggested that the combination of 5-FU and propolis extracts had a synergic growth inhibitory effect in HT-29 cells, while with fluphenazine, only green and red propolis, at a concentration of 100 μg/mL, presented synergism.
Published: 2023
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24. Evaluation of Antioxidant and Anticancer Activity of Mono- and Polyfloral Moroccan Bee Pollen by Characterizing Phenolic and Volatile Compounds.

Author: Aylanc V, Larbi S, Calhelha R, Barros L, Rezouga F, Rodríguez-Flores MS, Seijo MC, El Ghouizi A, Lyoussi B, Falcão SI, and Vilas-Boas M
Subjects: Animals, Bees, Gas Chromatography-Mass Spectrometry, Mass Spectrometry, Phenols chemistry, Pollen chemistry, Antioxidants chemistry, Flavonoids chemistry
Abstract: Bee pollen is frequently characterized as a natural source of bioactive components, such as phenolic compounds, which are responsible for its pharmaceutical potential and nutritional properties. In this study, we evaluated the bioactive compound contents of mono- and polyfloral bee pollen samples using spectroscopic and chromatographic methods and established links with their antioxidant and antitumor activity. The findings demonstrated that the botanical origin of bee pollen has a remarkable impact on its phenolic (3-17 mg GAE/g) and flavonoid (0.5-3.2 mg QE/g) contents. Liquid chromatography-mass spectrometry analysis revealed the presence of 35 phenolic and 13 phenylamide compounds in bee pollen, while gas chromatography-mass spectrometry showed its richness in volatiles, such as hydrocarbons, fatty acids, alcohols, ketones, etc. The concentration of bioactive compounds in each sample resulted in a substantial distinction in their antioxidant activity, DPPH (EC 50 : 0.3-0.7 mg/mL), ABTS (0.8-1.3 mM Trolox/mg), and reducing power (0.03-0.05 mg GAE/g), with the most bioactive pollens being the monofloral samples from Olea europaea and Ononis spinosa . Complementarily, some samples revealed a moderate effect on cervical carcinoma (GI 50 : 495 μg/mL) and breast adenocarcinoma (GI 50 : 734 μg/mL) cell lines. This may be associated with compounds such as quercetin- O -diglucoside and kaempferol-3- O -rhamnoside, which are present in pollens from Olea europaea and Coriandrum , respectively. Overall, the results highlighted the potentiality of bee pollen to serve health-promoting formulations in the future.
Published: 2023
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25. Analytical methods for honeybee venom characterization.

Author: El Mehdi I, Falcão SI, Boujraf S, Mustapha H, Campos MG, and Vilas-Boas M
Abstract: The discovery of new drugs has benefited significantly from the development of research in venomics, increasing our understanding of the envenomation processes. It has been previously reported that honeybee venom (HBV) exhibits several pharmacological activities such as anti-inflammatory, antibacterial, antimutagenic, radioprotective, and anticancer activity and may inclusively act as a complementary treatment for SARS-CoV-2. It composition consists mainly on melittin, phospholipase A2, and apamin but other constituents such as hyaluronidase, mast cell degranulating peptide and secapin are also relevant for its bioactivity. However, and because HBV is not officially recognized as a drug, until now, the international community did not establish quality standards for it. To uncover its exact composition, and boost the discovery of HBV-derived drugs, a significant number of techniques were developed. In this review, a relevant overview of the so far published analytical methods for HBV characterization is organized with the aim to accelerate its future standardization. The literature search was performed within PubMed, Google Scholar, and Science Direct by selecting specific documents and exploring HBV evaluation., Competing Interests: There are no conflicts of interest., (Copyright: © 2022 Journal of Advanced Pharmaceutical Technology & Research.)
Published: 2022
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26. Arbutus Unedo Honey and Propolis Ameliorate Acute Kidney Injury, Acute Liver Injury, and Proteinuria via Hypoglycemic and Antioxidant Activity in Streptozotocin-Treated Rats.

Author: Touzani S, Al-Waili N, Imtara H, Aboulghazi A, Hammas N, Falcão S, Vilas-Boas M, Arabi IE, Al-Waili W, and Lyoussi B
Subjects: Animals, Antioxidants chemistry, Antioxidants pharmacology, Antioxidants therapeutic use, Blood Glucose metabolism, Hypoglycemic Agents therapeutic use, Liver metabolism, Male, Proteinuria pathology, Rats, Rats, Wistar, Streptozocin toxicity, Acute Kidney Injury chemically induced, Acute Kidney Injury drug therapy, Acute Kidney Injury metabolism, Honey, Propolis pharmacology, Propolis therapeutic use
Abstract: Background/aims: Honey and propolis have biological and therapeutic effects in various pathological and clinical conditions such as hyperglycemia and diabetes. However, the combined use of honey and propolis has not been reported. The study evaluated the protective effect of Arbutus unedo honey, propolis and their combination in streptozotocin (STR)-induced hyperglycemia, acute kidney injury (AKI), liver injury, dyslipidemia, and proteinuria in male Wistar rats., Methods: The study identified physicochemical characteristics, mineral and antioxidant content, and antioxidant activity in honey and propolis. Rats were assigned to five groups, with five rats in each group; control, STR-treated, STR-treated + honey (1g/kg/day), STR-treated + propolis (100 mg/day), and STR-treated + honey and propolis. On day 15, blood glucose, insulin, HBA1c, kidney function tests, liver enzymes, lipid profile, hemoglobin, and urine protein, creatinine, glucose, and electrolytes were analyzed. Liver, pancreas, and kidney tissues were studied histologically. The mineral component in honey and propolis was determined by atomic absorption spectrometry. Honey analysis was performed by HPLC. Chemical characterization of propolis was performed by LC/DAD/ESI-MS n . Measurement of blood and urine parameters was carried out with an automated analyzer (Architect c8000) and XT-1800i Automated Hematology Analyzer. Insulin concentration was determined by Elisa and insulin resistance was estimated by using HOMA-IR., Results: Honey and propolis contain a high quantity of antioxidants and exhibit in vitro antioxidant activity. In STR-treated rats, blood glucose, HBA1c, creatinine, blood urea, liver enzymes, and urine protein significantly increased compared to the control group (P<0.05), while insulin, hemoglobin, and body weight significantly decreased. Histological changes were evident in the pancreas, kidney, and liver tissues. These results indicated AKI, liver injury, and pancreatic injury, which was evident with reducing the number of the island of Langerhans and marked hyperglycemia. The use of honey and propolis significantly (P<0.05) attenuated liver and kidney injury, and proteinuria, and improved level of hemoglobin, HBA1c, and insulin toward the normal range. The combination of honey and propolis was more effective than honey or propolis individually (P<0.05)., Conclusion: the combination of propolis and honey can prevent STR-induced AKI, liver injury, proteinuria, dyslipidemia, anemia, hyperglycemia, and body weight loss, most likely by their hypoglycemic and antioxidant activities., Competing Interests: The authors declare that no conflicts of interest exist., (© Copyright by the Author(s). Published by Cell Physiol Biochem Press.)
Published: 2022
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27. Honeybee Venom Synergistically Enhances the Cytotoxic Effect of CNS Drugs in HT-29 Colon and MCF-7 Breast Cancer Cell Lines.

Author: Duarte D, Falcão SI, El Mehdi I, Vilas-Boas M, and Vale N
Abstract: 5-fluorouracil (5-FU) and doxorubicin (DOX) are potent anti-tumour agents commonly used for colon and breast cancer therapy, respectively. However, their clinical application is limited by their side effects and the development of drug resistance. Honeybee venom is a complex mixture of substances that has been reported to be effective against different cancer cells. Its active compound is melittin, a positively charged amphipathic peptide that interacts with the phospholipids of the cell membrane, forming pores that enable the internalization of small molecules with cytotoxic activities, . and consequently, causing cell death. Some central nervous system (CNS) drugs have recently demonstrated great anti-cancer potential, both in vitro, in vivo and in clinical trials, being promising candidates for drug repurposing in oncology. The present work evaluated the anti-cancer efficacy of honeybee venom in combination with chemotherapeutic or CNS drugs in HT-29 colon and MCF-7 breast cancer cell lines. The chemical characterization of a Portuguese sample of honeybee venom was done by LC-DAD-ESI/MSn analysis. For single treatments, cells were incubated with increasing concentrations of bee venom. For combination treatments, increasing concentrations of bee venom were first combined with the half-maximal inhibitory concentration (IC 50 ) of 5-FU and DOX, in HT-29 and MCF-7 cells, respectively. Cells were also treated with increasing concentrations of bee venom in combination with the IC 50 value of four CNS drugs (fluphenazine, fluoxetine, sertraline and thioridazine). Cytotoxicity was evaluated by MTT and SRB assays. The combination index (CI) value was calculated using CompuSyn software, based on the Chou-Talalay method. Synergy scores of different reference models (HSA, Loewe, ZIP and Bliss) were also calculated using SynergyFinder. The results demonstrate that honeybee venom is active against HT-29 colon and MCF-7 breast cancer cells, having better anti-tumour activity in MCF-7 cells. It was found that bee venom combined with 5-FU and fluphenazine in HT-29 cells resulted in less cytotoxic effects compared to the co-treatment of fluoxetine, sertraline and thioridazine plus bee venom, which resulted in less than 15% of viable cells for the whole range of concentrations. The combination of MCF-7 cells with repurposed drugs plus honeybee venom resulted in better anti-cancer efficacies than with DOX, notably for lower concentrations. A combination of fluoxetine and thioridazine plus honeybee venom resulted in less than 40% of viable cells for all ranges of concentrations. These results support that the combination of honeybee venom with repurposed drugs and chemotherapeutic agents can help improve their anti-cancer activity, especially for lower concentrations, in both cell lines. Overall, the present study corroborates the enormous bioactive potential of honeybee venom for colon and breast cancer treatments, both alone and in combination with chemotherapy or repurposed drugs.
Published: 2022
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28. Chemical, Cytotoxic, and Anti-Inflammatory Assessment of Honey Bee Venom from Apis mellifera intermissa .

Author: El Mehdi I, Falcão SI, Harandou M, Boujraf S, Calhelha RC, Ferreira ICFR, Anjos O, Campos MG, and Vilas-Boas M
Abstract: The venom from Apis mellifera intermissa , the main honey bee prevailing in Morocco, has been scarcely studied, despite its known potential for pharmacological applications. In the present work, we investigated the composition, the anti-inflammatory activity, and the venom's cytotoxic properties from fifteen honey bee venom (HBV) samples collected in three regions: northeast, central, and southern Morocco. The chemical assessment of honey bee venom was performed using LC-DAD/ESI/MS n , NIR spectroscopy and AAS spectroscopy. The antiproliferative effect was evaluated using human tumor cell lines, including breast adenocarcinoma, non-small cell lung carcinoma, cervical carcinoma, hepatocellular carcinoma, and malignant melanoma. Likewise, we assessed the anti-inflammatory activity using the murine macrophage cell line. The study provides information on the honey bee venom subspecies' main components, such as melittin, apamin, and phospholipase A2, with compositional variation depending on the region of collection. Contents of toxic elements such as cadmium, chromium, and plumb were detected at a concentration below 5 ppm, which can be regarded as safe for pharmaceutical use. The data presented contribute to the first study in HBV from Apis mellifera intermissa and highlight the remarkable antiproliferative and anti-inflammatory effects of HBV, suggesting it to be a candidate natural medicine to explore.
Published: 2021
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29. Assessment of the In Vivo and In Vitro Release of Chemical Compounds from Vespa velutina .

Author: Rodríguez-Flores MS, Falcão SI, Escuredo O, Queijo L, Seijo MC, and Vilas-Boas M
Subjects: Animals, Gas Chromatography-Mass Spectrometry, Pheromones analysis, Pheromones metabolism, Wasp Venoms analysis, Wasp Venoms metabolism, Wasps metabolism
Abstract: Vespa velutina has been rapidly expanding throughout Galicia since 2012. It is causing human health risks and well-known losses in the beekeeping sector. Control methods are scarce, unspecific, and ineffective. Semiochemicals are insect-derived chemicals that play a role in communication and they could be used an integrated pest management tool alternative to conventional pesticides. A previous determination of the organic chemical profile should be the first step in the study of these semiochemicals. HS-SPME in living individuals and the sting apparatus extraction followed by GC-MS spectrometry were combined to extract a possible profile of these compounds in 43 hornets from Galicia. The identified compounds were hydrocarbons, ketones, terpenes, and fatty acid, and fatty acid esters. Nonanal aldehyde appeared in important concentrations in living individuals. While pentadecane, 8-hexyl- and ethyl oleate were mainly extracted from the venom apparatus. Ketones 2-nonanone, 2-undecanone and 7-nonen-2-one, 4,8-dimethyl- were identified by both procedures, as was 1,7-Nonadiene, 4,8-dimethyl-. Some compounds were detected for the first time in V. velutina such as naphthalene, 1,6-dimethyl-4-(1-methylethyl). The chemical profile by caste was also characterized.
Published: 2021
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30. Volatile Profile of Portuguese Monofloral Honeys: Significance in Botanical Origin Determination.

Author: Machado AM, Antunes M, Miguel MG, Vilas-Boas M, and Figueiredo AC
Abstract: The volatile profiles of 51 samples from 12 monofloral-labelled Portuguese honey types were assessed. Honeys of bell heather, carob tree, chestnut, eucalyptus, incense, lavender, orange, rape, raspberry, rosemary, sunflower and strawberry tree were collected from several regions from mainland Portugal and from the Azores Islands. When available, the corresponding flower volatiles were comparatively evaluated. Honey volatiles were isolated using two different extraction methods, solid-phase microextraction (SPME) and hydrodistillation (HD), with HD proving to be more effective in the number of volatiles extracted. Agglomerative cluster analysis of honey HD volatiles evidenced two main clusters, one of which had nine sub-clusters. Components grouped by biosynthetic pathway defined alkanes and fatty acids as dominant, namely n -nonadecane, n -heneicosane, n -tricosane and n -pentacosane and palmitic, linoleic and oleic acids. Oxygen-containing monoterpenes, such as cis - and trans -linalool oxide (furanoid), hotrienol and the apocarotenoid α-isophorone, were also present in lower amounts. Aromatic amino acid derivatives were also identified, namely benzene acetaldehyde and 3,4,5-trimethylphenol. Fully grown classification tree analysis allowed the identification of the most relevant volatiles for discriminating the different honey types. Twelve volatile compounds were enough to fully discriminate eleven honey types (92%) according to the botanical origin.
Published: 2021
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31. Assessment of Bioactive Compounds under Simulated Gastrointestinal Digestion of Bee Pollen and Bee Bread: Bioaccessibility and Antioxidant Activity.

Author: Aylanc V, Tomás A, Russo-Almeida P, Falcão SI, and Vilas-Boas M
Abstract: Bee pollen and bee bread have always been regarded as excellent natural resources for application in food and pharmaceutical fields due to their rich nutrient content and diversity of bioactive compounds with health-improving properties. Extensive studies on both bee products as ingredients for a healthy diet were reported, although the data concerning their metabolization on the gastrointestinal tract is quite limited. Here, we report, at each digestive stage, the bioactive profile for both bee products, their bioaccessibility levels and the antioxidant activity evaluation. The findings indicated that the average bioaccessibility level of total phenolic and total flavonoid content for bee pollen was 31% and 25%, respectively, while it was 38% and 35% for bee bread. This was reflected in a decrease of their antioxidant capacity at the end of in vitro gastrointestinal digestion, both in free radicals scavenging capacity and in reducing power. Moreover, within the 35 phytochemicals identified, the most affected by gastrointestinal digestion were phenylamides, with a complete digestibility at the end of the intestinal phase. Overall, our results highlight that bioactive compounds in both raw products do not reflect the real amount absorbed in the intestine, being bee bread more accessible in bioactive content than bee pollen.
Published: 2021
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32. Chemical composition, antioxidant activity, and diuretic effect of Moroccan fresh bee pollen in rats.

Author: Ghouizi AE, Menyiy NE, Falcão SI, Vilas-Boas M, and Lyoussi B
Abstract: Aim: This study investigated the chemical composition, antioxidant activity, and diuretic effect of Moroccan aqueous extract of fresh bee pollen (AEFBP) in normal rats., Materials and Methods: The chemical composition of the extracted bioactive compounds was assessed using liquid chromatography with diode array detection coupled to electrospray ionization (ESI) tandem mass spectrometry (LC/DAD/ESI-MS n ). 2,2-diphenyl-1-picrylhydrazyl and the reducing power were used to assess the antioxidant properties of the extract, together with the determination of total phenols and flavonoids. To assess the diuretic effect, 20 normal rats were divided into five groups: The first was a control group administered by distilled water (10 mL/kg body weight), the second group received furosemide (10 mg/kg body weight), the third group received 100 mg/kg body weight of AEFBP, the fourth group received 250 mg/kg body weight of AEFBP, and the fifth group received 500 mg/kg body weight of AEFBP for 30 days. Toward the end of this experiment, urine output was measured, and plasma and urine were sampled to analyze creatinine, potassium, chloride, and sodium levels., Results: N 1 , N 5 , N 10 -tri-p-coumaroylspermidine is a spermidine derivative and was the main compound in this sample, in a total of 19 compounds identified, including flavonoids, glucoside flavonoids, and methylated derivatives. Force feeding with the AEFBP induced a significant increase in urine output and urinary electrolyte levels with a dependent dose-effect without changes in plasma electrolytes, whereas furosemide decreased plasma potassium., Conclusion: Moroccan fresh bee pollen extract contains flavonols and spermidines that induce a potential antioxidant activity related to significant diuretic effect without changes in plasma composition., (Copyright: © El Ghouizi, et al.)
Published: 2020
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33. Honey Volatiles as a Fingerprint for Botanical Origin-A Review on their Occurrence on Monofloral Honeys.

Author: Machado AM, Miguel MG, Vilas-Boas M, and Figueiredo AC
Subjects: Factor Analysis, Statistical, Flowers, Geography, Honey classification, Honey standards, Trees, Botany methods, Honey analysis, Volatile Organic Compounds analysis
Abstract: Honeys have specific organoleptic characteristics, with nutritional and health benefits, being highly appreciated by consumers, not only in food but also in the pharmaceutical and cosmetic industries. Honey composition varies between regions according to the surrounding flora, enabling its characterization by source or type. Monofloral honeys may reach higher market values than multifloral ones. Honey's aroma is very specific, resulting from the combination of volatile compounds present in low concentrations. The authentication of honey's complex matrix, according to its botanical and/or geographical origin, represents a challenge nowadays, due to the different sorts of adulteration that may occur, leading to the search for reliable marker compounds for the different monofloral honeys. The existing information on the volatiles of monofloral honeys is scarce and disperse. In this review, twenty monofloral honeys and honeydews, from acacia, buckwheat, chestnut, clover, cotton, dandelion, eucalyptus, fir tree, heather, lavender, lime tree, orange, pine, rape, raspberry, rhododendron, rosemary, strawberry tree, sunflower and thyme, were selected for volatile comparison purposes. Taking into consideration the country of origin, the technique of isolation and analysis, the five main volatiles from each of the honeys are compared. Whereas some compounds were found in several types of monofloral honey, and thus not considered good volatile markers, some monofloral honeys revealed characteristic volatile compounds independently of their provenance., Competing Interests: The authors declare no conflict of interest.
Published: 2020
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34. Dataset on free amino acids contents of Serra da Estrela PDO cheeses determined by UPLC-DAD-MS/MS.

Author: Reis Lima MJ, Santos AO, Falcão S, Fontes L, Teixeira-Lemos E, Vilas-Boas M, Veloso ACA, and Peres AM
Abstract: The composition of Serra da Estrela PDO cheeses (total fat, total protein, salt and free amino acids) was assessed using NIR spectrophotometry and UPLC-DAD-MS/MS. In total, 24 cheeses were acquired from 6 certified cheesemakers located in 5 different municipalities within the delimited PDO geographical region. Cheeses were produced from raw ewe milk of two autochthonous Portuguese sheep breeds, between November 2017 and March 2018, and were acquired after 45 days of maturation. The data include the mean (and respective standard deviations) levels of moisture (%), total fat (%), total protein (%) and salt (%), obtained by NIR spectrophotometry. As well the mean (and respective standard deviations) of free amino acids contents (mg/100 g of cheese, in wet basis) evaluated using a UPLC-DAD-MS/MS method are shown. The latter data include information regarding 8 essential free amino acids (histidine, leucine-isoleucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine) and 9 non-essential free amino acids (arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, proline, tyrosine and serine). Leucine and isoleucine, being isomers, were quantified together. Leucine-isoleucine, phenylalanine and serine were the most abundant essential free amino acids and cysteine, proline and asparagine were the most abundant non-essential free amino acids. Free amino acids contents depended on the cheese producer as well as on the production time-period., (© 2019 The Author(s).)
Published: 2019
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35. In Vitro Interactions of Moroccan Propolis Phytochemical's on Human Tumor Cell Lines and Anti-Inflammatory Properties.

Author: Falcão SI, Calhelha RC, Touzani S, Lyoussi B, Ferreira ICFR, and Vilas-Boas M
Subjects: Animals, Anti-Inflammatory Agents chemistry, Antineoplastic Agents, Phytogenic chemistry, Cell Proliferation drug effects, Cell Survival drug effects, HeLa Cells, Hep G2 Cells, Humans, MCF-7 Cells, Mice, Morocco, Phenols chemistry, Phytochemicals chemistry, RAW 264.7 Cells, Anti-Inflammatory Agents pharmacology, Antineoplastic Agents, Phytogenic pharmacology, Phenols pharmacology, Phytochemicals pharmacology, Propolis chemistry
Abstract: Propolis is a resin manufactured by bees through the mixture of plant exudates and waxes with secreted substances from their metabolism, resulting in a complex mixture of natural substances of which quality depends on the phytogeographic and climatic conditions around the hive. The present study investigated the contribution of phenolic compounds to the cytotoxic and anti-inflammatory activities of propolis. The phenolic composition was evaluated by liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry (LC/DAD/ESI-MS n ) analysis after phenolic extraction. The cytotoxicity of the extracts was checked using human tumor cell lines (MCF7- breast adenocarcinoma, NCI-H460- non-small cell lung carcinoma, HeLa- cervical carcinoma, HepG2- hepatocellular carcinoma, and MM127- malignant melanoma), as well as non-tumor cells (a porcine liver primary culture-PLP2). The anti-inflammatory activity was assessed using the murine macrophage (RAW 264.7) cell line. The results showed a composition rich in phenolic acids, such as caffeic and p-coumaric acid, as well as flavonoids, such as pinocembrin, pinobanksin, and pinobanksin-3-O-butyrate. Samples MP2 from Sefrou and MP3 from Moulay Yaâcoub presented a high concentration in phenolic compounds, while MP1 and MP4 from Boulemane and Immouzzer Mermoucha, respectively, showed similar composition with low bioactivity. The higher concentration of phenolic compound derivatives, which seems to be the most cytotoxic phenolic class, can explain the pronounced antitumor and anti-inflammatory activity observed for sample MP2.
Published: 2019
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36. Flavonoid Composition and Antitumor Activity of Bee Bread Collected in Northeast Portugal.

Author: Sobral F, Calhelha RC, Barros L, Dueñas M, Tomás A, Santos-Buelga C, Vilas-Boas M, and Ferreira IC
Subjects: Antineoplastic Agents pharmacology, Cell Line, Tumor, Cell Survival drug effects, Drug Screening Assays, Antitumor, Flavonoids pharmacology, Humans, Inhibitory Concentration 50, Portugal, Propolis pharmacology, Antineoplastic Agents chemistry, Flavonoids chemistry, Propolis chemistry
Abstract: Bee bread (BB) is a fermented mixture of plant pollen, honey, and bee saliva that worker bees use as food for larvae, and for young bees to produce royal jelly. In the present study, five BB samples, collected from Apis mellifera iberiensis hives located in different apiaries near Bragança, in the northeast region of Portugal, and one BB commercial sample were characterized by high-performance liquid chromatography coupled to a diode array detector and electrospray mass spectrometry (HPLC-DAD-ESI/MS) in terms of phenolic compounds, such as flavonoid glycoside derivatives. Furthermore, the samples were screened, using in vitro assays, against different human tumor cell lines, MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), HeLa (cervical carcinoma) and HepG2 (hepatocellular carcinoma), and also against non-tumor liver cells (porcine liver cells, PLP2). The main phenolic compounds found were flavonol derivatives, mainly quercetin, kaempferol, myricetin, isorhamnetin and herbacetrin glycoside derivatives. Thirty-two compounds were identified in the six BB samples, presenting BB1 and BB3 with the highest contents (6802 and 6480 µg/g extract, respectively) and the highest number of identified compounds. Two isorhamnetin glycoside derivatives, isrohamnetin- O -hexosyl- O -rutinoside and isorhamnetin- O -pentosyl-hexoside, were the most abundant compounds present in BB1; on the other hand, quercetin-3- O -rhamnoside was the most abundant flavonol in BB3. However, it was not possible to establish a correlation between the flavonoids and the observed low to moderate cytotoxicity (ranging from >400 to 68 µg/mL), in which HeLa and NCI-H460 cell lines were the most susceptible to the inhibition. To the authors' knowledge, this is the first report characterizing glycosidic flavonoids in BB samples, contributing to the chemical knowledge of this less explored bee product.
Published: 2017
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37. Chemical characterization, antioxidant, anti-inflammatory and cytotoxic properties of bee venom collected in Northeast Portugal.

Author: Sobral F, Sampaio A, Falcão S, Queiroz MJ, Calhelha RC, Vilas-Boas M, and Ferreira IC
Subjects: Animals, Cell Line, Cell Line, Tumor, Chromatography, Liquid, Drug Evaluation, Preclinical, Humans, Mice, Portugal, Spectrometry, Mass, Electrospray Ionization, Anti-Inflammatory Agents pharmacology, Antineoplastic Agents pharmacology, Antioxidants pharmacology, Bee Venoms pharmacology
Abstract: Bee venom (BV) or apitoxin is a complex mixture of substances with reported biological activity. In the present work, five bee venom samples obtained from Apis mellifera iberiensis from the Northeast Portugal (two different apiaries) were chemically characterized and evaluated for their antioxidant, anti-inflammatory and cytotoxic properties. The LC/DAD/ESI-MS(n) analysis of the samples showed that melittin was the most abundant compound, followed by phospholipase A2 and apamin. All the samples revealed antioxidant and anti-inflammatory activity but without a direct relation with any of the individual chemical components identified. The results highlight that there are specific concentrations (present in BV5) in which these compounds are more active. The BV samples showed similar cytotoxicity for all the tested tumour cell lines (MCF-7, NCI-H460, HeLa and HepG2), being MCF-7 and HeLa the most susceptible ones. Nevertheless, the studied samples seem to be suitable to treat breast, hepatocellular and cervical carcinoma because at the active concentrations, the samples were not toxic for non-tumour cells (PLP2). Regarding the non-small cell lung carcinoma, BV should be used under the toxic concentration for non-tumour cells. Overall, the present study corroborates the enormous bioactive potential of BV being the first report on samples from Portugal., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
Published: 2016
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38. The grafting of a thin layer of poly(sodium styrene sulfonate) onto poly(ε-caprolactone) surface can enhance fibroblast behavior.

Author: Rohman G, Huot S, Vilas-Boas M, Radu-Bostan G, Castner DG, and Migonney V
Subjects: Fibroblasts cytology, Photoelectron Spectroscopy, Spectrometry, X-Ray Emission, Spectroscopy, Fourier Transform Infrared, Surface Properties, Tissue Engineering, Polyesters chemistry, Polymers chemistry, Sulfonic Acids chemistry
Abstract: Poly(sodium styrene sulfonate) (pNaSS) was grafted onto poly(ε-caprolatone) (PCL) surfaces via ozonation and graft polymerization. The effect of ozonation and polymerization time, as well as the Mohr's salt concentration in the grafting solution, on the degree of grafting was investigated. The degree of grafting was determined through toluidine blue staining. The surface chemical change was characterized by attenuated total reflection Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. The result demonstrated that the grafting did not induce any degradation of PCL, and that pNaSS was grafted onto PCL as a thin and covalently stable layer. Furthermore, the modified PCL surface reveals a significant increase in the metabolic activity of fibroblastic cells, as well as a better cell spreading with higher adhesion strength. Consequently, bioactivity of PCL is greatly enhanced by immobilizing a thin layer of pNaSS onto its surface. The grafting of pNaSS is a promising approach to increase the bioactivity of PCL-based materials used in tissue engineering applications, such as ligament reconstruction.
Published: 2015
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39. Antioxidant activity of aminodiarylamines in the thieno[3,2-b]pyridine series: radical scavenging activity, lipid peroxidation inhibition and redox profile.

Author: Calhelha RC, Peixoto D, Vilas Boas M, Queiroz MJ, and Ferreira IC
Subjects: Amines chemical synthesis, Animals, Brain drug effects, Brain metabolism, Chromans pharmacology, Electrochemical Techniques, Free Radical Scavengers chemical synthesis, Linoleic Acid chemistry, Lipid Peroxidation drug effects, Nitrobenzenes chemistry, Pyridines chemical synthesis, Structure-Activity Relationship, Swine, Thiobarbiturates antagonists & inhibitors, Thiobarbiturates chemistry, Tissue Extracts metabolism, beta Carotene chemistry, Amines pharmacology, Free Radical Scavengers pharmacology, Pyridines pharmacology, Tissue Extracts chemistry
Abstract: The antioxidant activity of the aminodi(hetero)arylamines, prepared by C-N coupling of the methyl 3-aminothieno[3,2-b]pyridine-2-carboxylate with bromonitrobenzenes and further reduction of the obtained nitro compounds, was evaluated by chemical, biochemical and electrochemical assays. The aminodi(hetero)arylamine with the amino group ortho to the NH and a methoxy group in para, was the most efficient in radical scavenging activity (RSA, 63 µM) and reducing power (RP, 33 µM), while the aminodiarylamine with the amino group in para to the NH, gave the best results in β-carotene-linoleate system (41 µM) and inhibition of formation of thiobarbituric acid reactive substances in porcine brain cells homogenates (7 µM), with EC50 values even lower than those obtained for the standard trolox. This diarylamine also presented the lowest oxidation potential, lower than the one of trolox, and the highest antioxidant power in the electrochemical assays. The para substitution with an amino group enables higher antioxidant potential.
Published: 2014
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40. Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines.

Author: Calhelha RC, Falcão S, Queiroz MJ, Vilas-Boas M, and Ferreira IC
Subjects: Cell Death drug effects, Cell Line, Tumor, Dose-Response Relationship, Drug, Flavonoids pharmacology, Humans, Phenols pharmacology, Portugal, Propolis pharmacology
Abstract: With a complex chemical composition rich in phenolic compounds, propolis (resinous substance collected by Apis mellifera from various tree buds) exhibits a broad spectrum of biological activities. Recently, in vitro and in vivo data suggest that propolis has anticancer properties, but is the cytoxicity of propolis specific for tumor cells? To answer this question, the cytotoxicity of phenolic extracts from Portuguese propolis of different origins was evaluated using human tumor cell lines (MCF7-breast adenocarcinoma, NCI-H460-non-small cell lung carcinoma, HCT15-colon carcinoma, HeLa-cervical carcinoma, and HepG2-hepatocellular carcinoma), and non-tumor primary cells (PLP2). The studied propolis presented high cytotoxic potential for human tumor cell lines, mostly for HCT15. Nevertheless, excluding HCT15 cell line, the extracts at the GI50 obtained for tumor cell lines showed, in general, cytotoxicity for normal cells (PLP2). Propolis phenolic extracts comprise phytochemicals that should be further studied for their bioactive properties against human colon carcinoma. In the other cases, the proximity of the in vitro cytotoxic doses for tumor and normal cell lines should be confirmed by in vivo tests and may highlight the need for selection of specific compounds within the propolis extract.
Published: 2014
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41. Phenolic characterization of Northeast Portuguese propolis: usual and unusual compounds.

Author: Falcão SI, Vilas-Boas M, Estevinho LM, Barros C, Domingues MR, and Cardoso SM
Subjects: Chromatography, High Pressure Liquid, Flavonoids analysis, Portugal, Spectrometry, Mass, Electrospray Ionization, Phenols analysis, Plant Extracts analysis, Propolis chemistry
Abstract: In this study, an ethanolic extract from Portuguese propolis was prepared, fractionated by high-performance liquid chromatography, and the identification of the phenolic compounds was done by electrospray mass spectrometry in the negative mode. This technical approach allowed the identification of 37 phenolic compounds, which included not only the typical phenolic acids and flavonoids found in propolis from temperate zones but also several compounds in which its occurrence have never been referred to in the literature. Four of the novel phenolic compounds were methylated and/or esterified or hydroxylated derivatives of common poplar flavonoids, although six peculiar derivatives of pinocembrin/pinobanksin, containing a phenylpropanoic acid derivative moiety in their structure, were also identified. Furthermore, the Portuguese propolis sample was shown to contain a p-coumaric ester derivative dimer.
Published: 2010
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42. Effect of microwave heating with different exposure times on physical and chemical parameters of olive oil.

Author: Malheiro R, Oliveira I, Vilas-Boas M, Falcão S, Bento A, and Pereira JA
Subjects: Chlorophyll chemistry, Food Handling, Hydrogen-Ion Concentration, Olive Oil, Time Factors, Hot Temperature, Microwaves, Plant Oils chemistry
Abstract: This study reflects the effect of different microwave heating times (1, 3, 5, 10 and 15 min) on physical and chemical characteristics of three Portuguese olive oils from three protected designation of origin (PDO), "Azeite de Trás-os-Montes PDO", "Azeites da Beira Interior PDO", and "Azeite de Moura PDO". The parameters evaluated were free acidity, peroxide value, specific extinction coefficients (K232 and K270), color and chlorophylls and carotenoids content. A differential pulse voltammeter was also used to monitor the changes in alpha-tocopherol content. The results showed that microwave heating produce losses in the quality of the different analysed olive oils. The heating time did not promote the occurrence of hydrolysis in the samples since no changes in free acidity values were found. All other parameters were affected by exposure time in a similar way: in the first 3 min no marked changes were observed, after that the quality of the oil decrease significantly. The microwave heating time also affects the total chlorophylls, carotenoids and alpha-tocopherol contents which clearly decreased as long as the exposure time increases. After 15 min of heating the electrochemical signal, due to the alpha-tocopherol, disappear completely in the voltamogram.
Published: 2009
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