18 results on '"Ventrella V."'
Search Results
2. Positive predictive value for malignancy on surgical excision of breast lesions of uncertain malignant potential (B3) diagnosed by stereotactic vacuum-assisted needle core biopsy (VANCB): A large multi-institutional study in Italy
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Bianchi, S., Caini, S., Renne, G., Cassano, E., Ambrogetti, D., Cattani, M.G., Saguatti, G., Chiaramondia, M., Bellotti, E., Bottiglieri, R., Ancona, A., Piubello, Q., Montemezzi, S., Ficarra, G., Mauri, C., Zito, F.A., Ventrella, V., Baccini, P., Calabrese, M., and Palli, D.
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- 2011
- Full Text
- View/download PDF
3. Carbides formation in weld metal produced by recycling of titanium machining swarf
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Fagundes, J G, primary, Ventrella, V A, additional, Gallego, J, additional, Moreno, A M, additional, Jácome, J L, additional, and Bracarense, A Q, additional
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- 2019
- Full Text
- View/download PDF
4. Positive predictive value for malignancy on surgical excision of breast lesions of uncertain malignant potential (B3) diagnosed by stereotactic vacuum-assisted needle core biopsy (VANCB): a large multi-institutional study in Italy
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Bianchi, S, Caini, S, Renne, G, Cassano, E, Ambrogetti, D, Cattani, Mg, Saguatti, G, Chiaramondia, M, Bellotti, E, Bottiglieri, R, Ancona, A, Piubello, Q, Montemezzi, S, Ficarra, G, Mauri, C, Zito, Fa, Ventrella, V, Baccini, P, Calabrese, M, Palli, D, Antonacci, Cm, Bersiga, A, Carli, F, Carrillo, G, Castellano, Isabella, Dante, S, Di Loreto, C, Di Stefano, D, Fanelli, G, Ferrero, G, Galasso, Mg, Giardina, E, Grillo, L, Laurino, L, Naccarato, G, Querzoli, P, Sapino, Anna, Vezzosi, V, Amadori, S, Balestrieri, N, Bazzocchi, M, Belotti, E, Cilotti, A, Corcione, S, Durando, M, Faedda, C, Festa, R, Guerrieri, A, Ingianna, D, Maggian, P, Mariscotti, G, Massa, T, Mattei, M, Rizzo, Mf, Scalabrin, U, Trasente, I, and Ventrella, V.
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medicine.medical_specialty ,Positive predictive value ,Percutaneous ,B3 lesions, Positive predictive value, Surgical excision, Stereotactic vacuum-assisted needle core biopsy, Non-palpable lesion, Breast ,B3 lesions, Breast, Non-palpable lesion, Positive predictive value, Stereotactic vacuum-assisted needle core biopsy, Surgical excision ,B3 lesions ,Surgical excision ,Stereotactic vacuum-assisted needle core biopsy ,Non-palpable lesion ,Breast ,Breast Neoplasms ,Malignancy ,NO ,Text mining ,Predictive Value of Tests ,Biopsy ,medicine ,Humans ,Early Detection of Cancer ,Retrospective Studies ,medicine.diagnostic_test ,business.industry ,Biopsy, Needle ,Retrospective cohort study ,Stereotactic vacuum-assisted needle core ,General Medicine ,medicine.disease ,Italy ,Predictive value of tests ,Radiological weapon ,Surgery ,Female ,Radiology ,business - Abstract
Percutaneous core biopsy (CB) has been introduced to increase the ability of accurately diagnosing breast malignancies without the need of resorting to surgery. Compared to conventional automated 14 gauge needle core biopsy (NCB), vacuum-assisted needle core biopsy (VANCB) allows obtaining larger specimens and has recognized advantages particularly when the radiological pattern is represented by microcalcifications. Regardless of technical improvements, a small percentage of percutaneous CBs performed to detect breast lesions are still classified, according to European and UK guidelines, in the borderline B3 category, including a group of heterogeneous lesions with uncertain malignant potential. We aimed to assess the prevalence and positive predictive values (PPV) on surgical excision (SE) of B3 category (overall and by sub-categories) in a large series of non-palpable breast lesions assessed through VANCB, also comparison with published data on CB. Overall, 26,165 consecutive stereotactic VANCB were identified in 22 Italian centres: 3107 (11.9%) were classified as B3, of which 1644 (54.2%) proceeded to SE to establish a definitive histological diagnosis of breast pathology. Due to a high proportion of microcalcifications as main radiological pattern, the overall PPV was 21.2% (range 10.6%-27.3% for different B3 subtypes), somewhat lower than the average value (24.5%) from published studies (range 9.9%-35.1%). Our study, to date the largest series of B3 with definitive histological assessment on SE, suggests that B3 lesions should be referred for SE even if VANCB is more accurate than NCB in the diagnostic process of non-palpable, sonographically invisible breast lesions.
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- 2011
5. Morphological parameters of flat epithelial atypia (FEA) in stereotactic vacuum-assisted needle core biopsies do not predict the presence of malignancy on subsequent surgical excision
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Bianchi, S, Bendinelli, B, Castellano, I, Piubello, Quirino, Renne, G, Cattani, Mg, Di Stefano, D, Carrillo, G, Laurino, L, Bersiga, A, Giardina, C, Dante, S, Di Loreto, C, Quero, C, Antonacci, Cm, Palli, D, VANCB Study Group, Ferrero, G, Piubello, Q, Querzoli, P, Sapino, A, Vezzosi, V, Amadori, S, Ambrogetti, D, Balestrieri, N, Bazzocchi, M, Bodini, Mt, Cassano, E, Durando, M, Festa, R, Guerrieri, Am, Maggian, P, Mariscotti, G, Mattei, M, Montemezzi, Stefania, Saguatti, G, Scalabrin, U, Trasente, I, and Ventrella, V.
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Pathology ,medicine.medical_specialty ,Vacuum ,Breast, Flat epithelial atypia, Stereotactic vacuum-assisted needle core biopsy, Surgical excision, Malignancy ,Biopsy ,diagnosis/pathology ,Breast Neoplasms ,Malignancy ,NO ,methods ,Pathology and Forensic Medicine ,Stereotaxic Techniques ,Breast cancer ,Predictive Value of Tests ,Ductal ,medicine ,Atypia ,Carcinoma ,Humans ,Clinical significance ,Breast ,Molecular Biology ,Large-Core Needle ,methods, Breast Neoplasms ,diagnosis/pathology, Breast ,pathology, Carcinoma in Situ ,diagnosis/pathology, Carcinoma ,diagnosis/pathology, Epithelial Cells ,pathology, Female, Humans, Hyperplasia ,diagnosis/pathology, Logistic Models, Middle Aged, Predictive Value of Tests, Stereotaxic Techniques, Vacuum ,Hyperplasia ,medicine.diagnostic_test ,business.industry ,Stereotactic vacuum-assisted needle core biopsy ,Carcinoma, Ductal, Breast ,Surgical excision ,Epithelial Cells ,Cell Biology ,General Medicine ,Middle Aged ,medicine.disease ,Flat epithelial atypia ,Logistic Models ,Predictive value of tests ,Stereotaxic technique ,pathology ,Female ,Biopsy, Large-Core Needle ,Breast, Flat epithelial atypia, Malignancy, Stereotactic vacuum-assisted needle core biopsy, Surgical excision ,business ,Carcinoma in Situ - Abstract
Flat epithelial atypia (FEA) may represent the earliest precursor of low-grade breast cancer and often coexists with more advanced atypical proliferative breast lesions such as atypical ductal hyperplasia (ADH) and lobular intraepithelial neoplasia (LIN). The present study aims to investigate the association between morphological parameters of FEA and presence of malignancy at surgical excision (SE) and the clinical significance of the association of FEA with ADH and/or LIN. This study included 589 cases of stereotactic 11-gauge vacuum-assisted needle core biopsy (VANCB), reporting a diagnosis of FEA, ADH or LIN with subsequent SE from 14 pathology departments in Italy. Available slides were reviewed, with 114 (19.4 %) showing a malignant outcome at SE. Among the 190 cases of pure FEA, no statistically significant association emerged between clinical–pathological parameters of FEA and risk of malignancy. Logistic regression analyses showed an increased risk of malignancy according to the extension of ADH among the 275 cases of FEA associated with ADH (p = 0.004) and among the 34 cases of FEA associated with ADH and LIN (p = 0.02). In the whole series, a statistically significant increased malignancy risk emerged according to mammographic R1–R3/R4–R5 categories (OR = 1.56; p = 0.04), extension (OR = 1.24; p = 0.04) and grade (OR = 1.94; p = 0.004) of cytological atypia of FEA. The presence of ADH was associated with an increased malignancy risk (OR = 2.85; p < 0.0001). Our data confirm the frequent association of FEA with ADH and/or LIN. A diagnosis of pure FEA on VANCB carries a 9.5 % risk of concurrent malignancy and thus warrants follow-up excision because none of the clinical–pathological parameters predicts which cases will present carcinoma on SE.
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- 2012
6. Organotin Effects in Different Phyla: Discrepancies and Similarities
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Alessandra Pagliarani, Salvatore Nesci, Vittoria Ventrella, Fabiana Trombetti, PAGLIARANI A., TROMBETTI F., VENTRELLA V., Pagliarani A., Nesci S., Trombetti F., and Ventrella V.
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Phylum ,Evolutionary biology ,Chemistry ,ANIMAL PHYLA ,TOXICITY MECHANISMS ,ORGANOTIN EFFECTS ,DISCREPANCIES ,SIMILARITIES - Abstract
Most of the biological effects displayed by organotin contaminants, among which trisubsituted species are especially toxic, have increasingly been found to exhibit astonishing analogies in different taxa. While similarities can be perceived from prokaryotes to mammals, different modes and extent of biochemical and biological effects were described in different cells, tissues and species. A broad susceptibility range to organotins emerges from literature. Aquatic biota are mainly affected by organotins as environmental water and sediments act as storage site. Endocrine and lipid homeostasis perturbations span from Mollusks, where first gender changes (imposex) referable to environmental organotin contamination was pointed out, to Mammals, where organotins play the role of environmental obesogens. Organotin immunotoxicity, elicited in various invertebrate Phyla, also affects humans. Inhibition of key membrane-bound enzyme complexes such as Na,K-ATPase and FOF1 complexes, thus affecting hydromineral balance, energy production and related effects, are known to occur in a wide variety of organisms. Mitochondria and all membrane functions apparently represent a preferred target of these lipophilic toxicants. Highly conserved action mechanisms could be involved in the observed effects: organotin binding to nuclear receptors, membrane components and intracellular proteins as well as DNA damage may represent widely shared action modes of these compounds. On the other hand the different response and even the refractoriness to these toxicants shown at different biological levels may mirror biochemical and physiological selectivity of signalling pathways, biomembranes and intracellular protein components.
- Published
- 2012
7. Mitochondrial F1FO-ATPase and permeability transition pore response to sulfide in the midgut gland of Mytilus galloprovincialis
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Fabiana Trombetti, Vittoria Ventrella, Cristina Algieri, Micaela Fabbri, Alessandra Pagliarani, Salvatore Nesci, DIPARTIMENTO DI SCIENZE MEDICHE VETERINARIE, Facolta' di MEDICINA VETERINARIA, AREA MIN. 05 - Scienze biologiche, Da definire, Algieri C., Nesci S., Trombetti F., Fabbri M., Ventrella V., and Pagliarani A.
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0301 basic medicine ,Sulfide ,Bioenergetics ,Mytilus galloprovinciali ,Mitochondrion ,Biochemistry ,Cofactor ,F1FO-ATPase ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry.chemical_classification ,030102 biochemistry & molecular biology ,biology ,MPTP ,Midgut gland ,Mitochondria ,Mytilus galloprovincialis ,Permeability transition pore ,General Medicine ,Enzyme assay ,030104 developmental biology ,Enzyme ,chemistry ,Mitochondrial permeability transition pore ,biology.protein ,Biophysics - Abstract
open 6 no The molecular mechanisms which rule the formation and opening of the mitochondrial permeability transition pore (mPTP), the lethal mechanism which permeabilizes mitochondria to water and solutes and drives the cell to death, are still unclear and particularly little investigated in invertebrates. Since Ca2+ increase in mitochondria is accompanied by mPTP opening and the participation of the mitochondrial F1FO-ATPase in the mPTP is increasingly sustained, the substitution of the natural cofactor Mg2+ by Ca2+ in the F1FO-ATPase activation has been involved in the mPTP mechanism. In mussel midgut gland mitochondria the similar kinetic properties of the Mg2+- or Ca2+-dependent F1FO-ATPase activities, namely the same affinity for ATP and bi-site activation kinetics by the ATP substrate, in spite of the higher enzyme activity and coupling efficiency of the Mg2+-dependent F1FO-ATPase, suggest that both enzyme activities are involved in the bioenergetic machinery. Other than being a mitochondrial poison and environmental contaminant, sulfide at low concentrations acts as gaseous mediator and can induce post-translational modifications of proteins. The sulfide donor NaHS, at micromolar concentrations, does not alter the two F1FO-ATPase activities, but desensitizes the mPTP to Ca2+ input. Unexpectedly, NaHS, under the conditions tested, points out a chemical refractoriness of both F1FO-ATPase activities and a failed relationship between the Ca2+-dependent F1FO-ATPase and the mPTP in mussels. The findings suggest that mPTP role and regulation may be different in different taxa and that the F1FO-ATPase insensitivity to NaHS may allow mussels to cope with environmental sulfide. embargoed_20211117 Algieri C.; Nesci S.; Trombetti F.; Fabbri M.; Ventrella V.; Pagliarani A. Algieri C.; Nesci S.; Trombetti F.; Fabbri M.; Ventrella V.; Pagliarani A.
- Published
- 2020
8. Season and Cooking May Alter Fatty Acids Profile of Polar Lipids from Blue-Back Fish
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Sabrina Albonetti, Federica Farabegoli, Anna Badiani, Maurizio Pirini, Salvatore Nesci, Vittoria Ventrella, Farabegoli F., Nesci S., Ventrella V., Badiani A., Albonetti S., and Pirini M.
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0301 basic medicine ,Sprattus sprattus ,Nutritional quality ,Biochemistry ,03 medical and health sciences ,Blue-back fish ,Animals ,Cooking effect ,Cooking ,Food science ,Trachurus trachurus ,chemistry.chemical_classification ,030109 nutrition & dietetics ,biology ,Fatty Acids ,Organic Chemistry ,Sardine ,Fishes ,Fatty acid ,Sprat ,food and beverages ,Cell Biology ,Season influence ,biology.organism_classification ,Lipids ,Horse mackerel ,030104 developmental biology ,chemistry ,Docosahexaenoic acid ,Polar lipid ,lipids (amino acids, peptides, and proteins) ,Seasons ,Fatty acid composition ,Polyunsaturated fatty acid - Abstract
Polar lipids (PoL) represent a new promising dietary approach in the prevention and treatment of many human diseases, due to their potential nutritional value and unique biophysical properties. This study investigates the effects of catching season and oven baking on the fatty acid profiles (FAP) of PoL in four species of blue-back fish widely present in the North Adriatic Sea: anchovy (Engraulis encrasicholus), sardine (Sardina pilchardus), sprat (Sprattus sprattus), and horse mackerel (Trachurus trachurus). PoL levels (427-652 mg/100 g flesh) varied among the four species, with no significant seasonal variations within species. FAP of raw fillets were particularly high in polyunsaturated fatty acid (PUFA), especially docosahexaenoic acid (DHA) and EPA; total PUFA was constant in all species throughout the year, while long-chain n-3 polyunsaturated fatty acid (n-3 PUFA) rose in spring (except in sprat), especially due to the contribution of DHA. The FAP response for PoL to oven baking was species-specific and, among n-3 PUFA, DHA exhibited the greatest heat resistance; the influence of oven baking on FAP was found to be correlated with the catching season, especially for anchovy and sardine, while sprat PoL were not affected by cooking processes. The four species analyzed in this study presented very low n-6/n-3 fatty acid ratios and highly favorable nutritional indices, emphasizing their PoL qualities and promoting their role in increasing human n-3 PUFA intake. The four species can be considered as superior sources of n-3 PUFA and can be employed as supplements in functional food manufacturing and in pharmaceutical and cosmetic industries.
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- 2019
9. Characterization of metabolic profiles and lipopolysaccharide effects on porcine vascular wall mesenchymal stem cells
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Vittoria Ventrella, Chiara Bernardini, Fabiana Trombetti, Roberta Salaroli, Augusta Zannoni, Alessandra Pagliarani, Salvatore Nesci, Monica Forni, Nesci S., Bernardini C., Salaroli R., Zannoni A., Trombetti F., Ventrella V., Pagliarani A., and Forni M.
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Lipopolysaccharides ,0301 basic medicine ,Swine ,Physiology ,Cellular respiration ,Clinical Biochemistry ,Cell ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Glycolysis ,Aorta ,Cells, Cultured ,chemistry.chemical_classification ,Reactive oxygen species ,medicine.diagnostic_test ,ATP synthase ,biology ,Chemistry ,Mesenchymal stem cell ,lipopolysaccharide ,Mesenchymal Stem Cells ,Cell Biology ,metabolic profile ,Cell biology ,Oxidative Stress ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,cellular acidification ,biology.protein ,porcine vascular wall mesenchymal stem cells ,Reactive Oxygen Species ,Adenosine triphosphate - Abstract
The link between metabolic remodeling and stem cell fate is still unclear. To explore this topic, the metabolic profile of porcine vascular wall mesenchymal stem cells (pVW-MSCs) was investigated. At the first and second cell passages, pVW-MSCs exploit both glycolysis and cellular respiration to synthesize adenosine triphosphate (ATP), but in the subsequent (third to eighth) passages they do not show any mitochondrial ATP turnover. Interestingly, when the first passage pVW-MSCs are exposed to 0.1 or 10 μg/ml lipopolysaccharides (LPSs) for 4 hr, even if ATP synthesis is prevented, the spare respiratory capacity is retained and the glycolytic capacity is unaffected. In contrast, the exposure of pVW-MSCs at the fifth passage to 10 μg/ml LPS stimulates mitochondrial ATP synthesis. Flow cytometry rules out any reactive oxygen species (ROS) involvement in the LPS effects, thus suggesting that the pVW-MSC metabolic pattern is modulated by culture conditions via ROS-independent mechanisms.
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- 2019
10. Effect of tributyltin on mammalian endothelial cell integrity
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Chiara Bernardini, Vittoria Ventrella, Maria Laura Bacci, G. Botelho, Monica Forni, Augusta Zannoni, Botelho, G, Bernardini, C., Zannoni, A., Ventrella, V., Bacci, M.L., and Forni, M.
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Time Factors ,Adhesion molecule ,Endothelium ,Cell Survival ,Swine ,Physiology ,Health, Toxicology and Mutagenesis ,Apoptosis ,Biology ,Toxicology ,Occludin ,Biochemistry ,Monocytes ,Tight Junctions ,Necrosis ,chemistry.chemical_compound ,Endothelial cell ,Cell Adhesion ,medicine ,Animals ,VCAM-1 ,Cell adhesion ,Cells, Cultured ,Tight junction ,Inflammation ,ICAM-1 ,Tight Junction Proteins ,Dose-Response Relationship, Drug ,Cell adhesion molecule ,Tributyltin ,Endothelial Cells ,Cell Biology ,General Medicine ,Coculture Techniques ,Health, Toxicology and Mutagenesi ,Cell biology ,Endothelial stem cell ,medicine.anatomical_structure ,Gene Expression Regulation ,chemistry ,Trialkyltin Compounds ,Adhesion molecules ,Cell Adhesion Molecules - Abstract
Tributyltin (TBT), is a man-made pollutants, known to accumulate along the food chain, acting as an endocrine disruptor in marine organisms, with toxic and adverse effects in many tissues including vascular system. Based on the absence of specific studies of TBT effects on endothelial cells, we aimed to evaluate the toxicity of TBT on primary culture of porcine aortic endothelial cells (pAECs), pig being an excellent model to study human cardiovascular disease. pAECs were exposed for 24h to TBT (100, 250, 500, 750 and 1000nM) showing a dose dependent decrease in cell viability through both apoptosis and necrosis. Moreover the ability of TBT (100 and 500nM) to influence endothelial gene expression was investigated at 1, 7 and 15h of treatment. Gene expression of tight junction molecules, occludin (OCLN) and tight junction protein-1 (ZO-1) was reduced while monocyte adhesion and adhesion molecules ICAM-1 and VCAM-1 (intercellular adhesion molecule-1 and vascular cell adhesion molecule-1) levels increased significantly at 1h. IL-6 and estrogen receptors 1 and 2 (ESR-1 and ESR-2) mRNAs, after a transient decrease, reached the maximum levels after 15h of exposure. Finally, we demonstrated that TBT altered endothelial functionality greatly increasing monocyte adhesion. These findings indicate that TBT deeply alters endothelial profile, disrupting their structure and interfering with their ability to interact with molecules and other cells.
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- 2015
11. Long-chain PUFA enrichment in microalgae and metabolic dynamics in Tapes philippinarum larvae
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Fabiana Trombetti, Alessio Bonaldo, Aurelio Zentilin, Maurizio Pirini, Pier Paolo Gatta, Vittoria Ventrella, Alessandra Pagliarani, Salvatore Nesci, Ventrella, V, Gatta, P.P., Zentilin, A., Pagliarani, A., Trombetti, F., Bonaldo, A., Nesci, S., and Pirini, M.
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0301 basic medicine ,Thalassiosira pseudonana ,PUFA enrichment ,Aquatic Science ,Clam ,03 medical and health sciences ,Biotransformation ,Microalgal mixture ,Food science ,Animal nutrition ,Tetraselmis ,Shellfish ,chemistry.chemical_classification ,biology ,Fatty acid ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,Tapes philippinarum ,030104 developmental biology ,Biochemistry ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Composition (visual arts) ,lipids (amino acids, peptides, and proteins) ,NMI fatty acid ,Fatty acid composition ,Polyunsaturated fatty acid - Abstract
The possibility of increasing n-3 and n-6 long-chain polyunsaturated fatty acids (PUFA) content in microalgal mixtures used to feed Tapes philippinarum larvae was explored by lowering culture temperature from 26 to 14 °C. Although fatty acid composition of different microalgal species has a genetic basis, the algal cultures grown at 14 °C significantly increased the content of long-chain n-3 PUFA in Isocrysis galbana and in Thalassiosira pseudonana, while in Tetraselmis tetrathelo, the PUFA increase only involved shorter chain PUFA, namely 16:4n-3 and 18:4n-3. However, larvae fed on the PUFA enriched microalgal mixture did not show improvements in growth and survival performances with respect to the control group fed the microalgal mixture grown at 26 °C. From a biochemical perspective, two key aspects emerged from the results: (i) clam larvae have adequate biotransformation and selection skills to adjust fatty acid profile to their requirements as they can even modulate the incorporation of essential long-chain PUFA as 20:5n-3 and 22:6n-3 when the dietary supply exceeds the physiological requirements; (ii) bivalve can biosynthesize non-methylene-interrupted dienoic (NMID) fatty acids as confirmed by the constancy of relative proportion with larvae growth in spite of the NMID fatty acid absence in the diet.
- Published
- 2016
12. Novel Drugs Targeting the c-Ring of the F1FO-ATP Synthase
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PAGLIARANI, ALESSANDRA, NESCI, SALVATORE, VENTRELLA, VITTORIA, Pagliarani, Alessandra, Nesci, S, and Ventrella, V
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Models, Molecular ,Protein Conformation ,Drug Discovery ,Organotin Compounds ,Quinolines ,Animals ,Humans ,Macrolides ,Molecular Targeted Therapy ,Enzyme Inhibitors ,Mitochondrial Proton-Translocating ATPases ,ATP synthase, c-ring, diarylquinolines, drug target, enzyme inhibition, macrolides, organotins - Abstract
Increasing evidence highlights the role of the ATP synthase/hydrolase, also known as F1FO-complex, as key molecular and enzymatic switch between cell life and death, thus increasing the enzyme attractiveness as drug target in pharmacology. Being inhibition of ATP production usually linked to antiproliferative properties, drugs targeting the enzyme complex have been mainly considered to fight pathogen parasites and cancer. In recent years, a number of natural macrolides, produced by bacterial fermentation and structurally related to the classical enzyme inhibitor oligomycin, have been shown to bind to the membrane-embedded FO sector and to inhibit the enzyme complex by an oligomycin-like mechanism, namely by interacting with the c-ring. Other than natural macrolide antibiotics, which display variegated inhibition power on different F1FO-complexes, synthetic compounds from the diarylquinoline and organotin families also target the c-ring and strongly inhibit the enzyme. Bioinformatic insights address drug design to target FO subunits. Additionally, the possible modulation of the drug inhibition power, by amino acid substitutions or post-translational modifications of c-subunits, adds further interest to the target. The present survey on compounds targeting the c-ring and bi-directionally blocking the transmembrane proton flux which drives ATP synthesis/hydrolysis, discloses new therapeutic options to fight cancer and infections sustained by therapeutically recalcitrant microorganisms. Additionally, c-ring targeting compounds may constitute new tools to eradicate undesired biofilms and to address at the molecular level the therapy of mammalian diseases linked to mitochondrial dysfunctions. In summary, studies on the only partially known molecular interactions within the c-ring of the F1FO-complex may renew hope to counteract mammalian diseases.
- Published
- 2015
13. Yessotoxin, a shellfish biotoxin, is a potent inducer of the permeability transition in isolated mitochondria and intact cells
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Fabiana Trombetti, Patrizia Ciminiello, Vittoria Ventrella, Paolo Bernardi, Cristina Bianchi, Alessia Angelin, Giovanna Parenti Castelli, Ernesto Fattorusso, Anna Borgatti, Giorgio Lenaz, Romana Fato, BIANCHI C., FATO R., ANGELIN A., TROMBETTI F., VENTRELLA V., BORGATTI A., FATTORUSSO E., CIMINIELLO P., BERNARDI P., LENAZ G., and PARENTI CASTELLI G.
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Male ,Time Factors ,Voltage-dependent anion channel ,animal structures ,Biophysics ,Mollusk Venoms ,Mitochondria, Liver ,Biology ,Mitochondrion ,Biochemistry ,Rat liver mitochondria ,Permeability ,Membrane Potentials ,Yessotoxin ,chemistry.chemical_compound ,Liver Neoplasms, Experimental ,Oxygen Consumption ,Ethers, Cyclic ,Cell Line, Tumor ,Cyclosporin a ,Animals ,Enzyme Inhibitors ,Rats, Wistar ,Inner mitochondrial membrane ,Fluorescent Dyes ,Mitochondrial permeability transition ,Molecular Structure ,Rhodamines ,Oxocins ,Depolarization ,Intracellular Membranes ,Cell Biology ,Bivalvia ,Rats ,Mitochondrial permeability transition pore ,chemistry ,Cyclosporine ,biology.protein ,Calcium ,Diarrhetic shellfish poisoning - Abstract
The diarrhetic poisoning by bivalve molluscs, diarrhetic shellfish poisoning, is due to consumption of mussels containing biotoxins produced by some Dinoflagellate species. Toxic effects of yessotoxin (YTX) include morphological alterations of mitochondria from heart and liver but the biochemical basis for these alterations is completely unknown.This paper demonstrates that YTX is a very powerful compound that opens the permeability transition pore (PTP) of the inner mitochondrial membrane of rat liver mitochondria at nanomolar concentrations. The effect requires the presence of a permissive level of calcium, by itself incapable of opening the pore. The direct effect of YTX on PTP is further confirmed by the inhibition exerted by cyclosporin A (CsA) that is known as a powerful inhibitor of PTP opening. Moreover, YTX induces membrane depolarization as shown by the quenching of tetramethylrhodamine methyl ester (TMRM), also prevented by the addition of CsA. YTX caused PTP opening in Morris Hepatoma 1C1 cells, as shown by the occurrence of CsA-sensitive depolarization within minutes of the addition of submicromolar concentrations of the toxin. These results provide a biochemical basis for the mitochondrial alterations observed in the course of intoxication with YTX, offering the first clue into the pathogenesis of diseases caused by YTX, and providing a novel tool to study the PTP in situ.
- Published
- 2004
14. The Evaluation of the Effects of Dietary Vitamin E or Selenium on Lipid Oxidation in Rabbit Hamburgers: Comparing TBARS and Hexanal SPME-GC Analyses.
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Trombetti F, Minardi P, Mordenti AL, Badiani A, Ventrella V, and Albonetti S
- Abstract
The effects and specificity of dietary supplementation of EconomasE
TM (EcoE), mainly consisting of organic selenium (0.15 or 0.30 mg/kg feed; Se) or of vitamin E (100 or 200 mg/kg feed; VE), on lipid oxidation were evaluated in rabbit hamburgers during refrigerated storage. Oxidation data obtained by thiobarbituric acid-reactive substances (TBARS) spectrophotometric analysis and solid-phase microextraction (SPME) coupled with gas chromatography (GC) to determine hexanal content were compared. The relationships between oxidation levels, colour and pH and the discriminability of EcoE- or VE-treatment compared with control were also examined. TBARS content decreased in both VE and EcoE groups, while hexanal showed lower values only in the VE100 dietary group. The colour parameters were compatible with acceptable product quality and seemed to parallel the TBARS values up to the second day storage. Both VE and EcoE improved oxidative stability without affecting the sensory properties, but the VE effect appeared to more specifically hamper lipid oxidation, as evidenced by the determination and quantification of hexanal, a specific product of fatty acid peroxidation.- Published
- 2022
- Full Text
- View/download PDF
15. Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology.
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Nesci S, Trombetti F, Pagliarani A, Ventrella V, Algieri C, Tioli G, and Lenaz G
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Under aerobic conditions, mitochondrial oxidative phosphorylation (OXPHOS) converts the energy released by nutrient oxidation into ATP, the currency of living organisms. The whole biochemical machinery is hosted by the inner mitochondrial membrane (mtIM) where the protonmotive force built by respiratory complexes, dynamically assembled as super-complexes, allows the F
1 FO -ATP synthase to make ATP from ADP + Pi. Recently mitochondria emerged not only as cell powerhouses, but also as signaling hubs by way of reactive oxygen species (ROS) production. However, when ROS removal systems and/or OXPHOS constituents are defective, the physiological ROS generation can cause ROS imbalance and oxidative stress, which in turn damages cell components. Moreover, the morphology of mitochondria rules cell fate and the formation of the mitochondrial permeability transition pore in the mtIM, which, most likely with the F1 FO -ATP synthase contribution, permeabilizes mitochondria and leads to cell death. As the multiple mitochondrial functions are mutually interconnected, changes in protein composition by mutations or in supercomplex assembly and/or in membrane structures often generate a dysfunctional cascade and lead to life-incompatible diseases or severe syndromes. The known structural/functional changes in mitochondrial proteins and structures, which impact mitochondrial bioenergetics because of an impaired or defective energy transduction system, here reviewed, constitute the main biochemical damage in a variety of genetic and age-related diseases.- Published
- 2021
- Full Text
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16. Mercury and protein thiols: Stimulation of mitochondrial F 1 F O -ATPase and inhibition of respiration.
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Nesci S, Trombetti F, Pirini M, Ventrella V, and Pagliarani A
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- Adenosine Triphosphate pharmacology, Animals, Arsenicals, Cell Respiration drug effects, Dithioerythritol pharmacology, Electron Transport drug effects, Enzyme Activation drug effects, Kinetics, Magnesium pharmacology, Mitochondria drug effects, Mitochondrial Proteins metabolism, Sus scrofa, Mercury pharmacology, Mitochondria enzymology, Proton-Translocating ATPases metabolism, Sulfhydryl Compounds pharmacology
- Abstract
In spite of the known widespread toxicity of mercury, its impact on mitochondrial bioenergetics is a still poorly explored topic. Even if many studies have dealt with mercury poisoning of mitochondrial respiration, as far as we are aware Hg
2+ effects on individual complexes are not so clear. In the present study changes in swine heart mitochondrial respiration and F1 FO -ATPase (F-ATPase) activity promoted by micromolar Hg2+ concentrations were investigated. Hg2+ was found to inhibit the respiration of NADH-energized mitochondria, whereas it was ineffective when the substrate was succinate. Interestingly, the same micromolar Hg2+ doses which inhibited the NADH-O2 activity stimulated the F-ATPase, most likely by interacting with adjacent thiol residues. Accordingly, Hg2+ dose-dependently decreased protein thiols and all the elicited effects on mitochondrial complexes were reversed by the thiol reducing agent DTE. These findings clearly indicate that Hg2+ interacts with Cys residues of these complexes and differently modulate their functionality by modifying the redox state of thiol groups. The results, which cast light on some implications of metal-thiol interactions up to now not fully explored, may contribute to clarify the molecular mechanisms of mercury toxicity to mitochondria., (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)- Published
- 2016
- Full Text
- View/download PDF
17. Body mass index and serum proteomic profile in breast cancer and healthy women: a prospective study.
- Author
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Garrisi VM, Tufaro A, Trerotoli P, Bongarzone I, Quaranta M, Ventrella V, Tommasi S, Giannelli G, and Paradiso A
- Subjects
- Adult, Alcohol Drinking adverse effects, Breast Neoplasms etiology, Breast Neoplasms pathology, Case-Control Studies, Cell Transformation, Neoplastic metabolism, Female, Humans, Lymphatic Metastasis, Middle Aged, Prospective Studies, Proteome genetics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tumor Burden, Body Mass Index, Breast Neoplasms blood, Cell Transformation, Neoplastic genetics, Postmenopause, Proteome metabolism
- Abstract
Epidemiological studies suggest a possible association between BMI, diagnosis and clinical-pathological breast cancer characteristics but biological bases for this relationship still remain to be ascertained. Several biological mechanisms play a role in the genesis and progression of breast cancer. This study aimed to investigate relationships between BMI and breast cancer diagnosis/progression in a Southern Italian population and to try to interpret results according to the serum proteomic profile of healthy and breast cancer patients. BMI, presence or absence of breast cancer and its clinical-pathological characteristics were analyzed in a series of 300 breast cancer women and compared with those of 300 healthy women prospectively. To investigate whether obesity is associated with alterations in serum protein profile, SELDI-ToF approach was applied.Alcohol consumption (22.7% vs 11.3%; p<0.001) and postmenopausal status (65.7% vs 52%; p<0.001) but not BMI resulted significantly different in patients vs controls. Conversely, BMI was significantly associated with a larger-tumour size (BMI> = 30 respect to normal weight: OR = 2.49, 95% CI 1.25-4.99, p = 0.0098) and a higher probability of having positive axillary lymph node (OR = 3.67, CI 95% 2.16-6.23, p<0.0001). Multivariate analysis confirmed the association of breast cancer diagnosis with alcohol consumption (OR = 2.28;CI 1.36-3.83; p<0.0018). Serum protein profile revealed the presence of significant (p-value <0,01) differentially expressed peaks m/z 6934, m/z 5066 in high BMI breast cancer patients vs healthy subjects and m/z 6934, m/z 3346 in high vs low BMI breast cancer patients.The analysis of pathological features of cancer indicates that normal weight women have a significantly higher probability of having a smaller breast cancer at time of diagnosis and negative axillary lymph nodes while increased BMI is associated with an altered protein profile in breast cancer patients. Further studies to identify specific proteins found in the serum and their role in breast cancerogenesis and progression are in progress.
- Published
- 2012
- Full Text
- View/download PDF
18. Response of rainbow trout gill Na+-ATPpase to T(3) and NaCl administration.
- Author
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Ventrella V, Pagliarani A, Trombetti F, Pirini M, Trigari G, and Borgatti AR
- Subjects
- Animals, Hydrogen-Ion Concentration, Microsomes physiology, Osmosis, Sodium Chloride pharmacology, Thyronines pharmacology, Water-Electrolyte Balance, Adenosine Triphosphatases metabolism, Cation Transport Proteins, Gills physiology, Oncorhynchus mykiss physiology
- Abstract
The effect of the administration of commercial diets supplemented with 9 mg kg(-1) 3,5,3'-triiodo-l-thyronine (T(3)) or 10% (w/w) NaCl was evaluated on the ouabain-insensitive Na+-ATPase activity in rainbow trout gill microsomes. The trial, carried out following the seasonal trend from March to mid-May, included a treatment phase in freshwater and a subsequent transfer to brackish water (22 per thousand salinity) where trout were not treated. pH dependence, apparent Km values for Mg(2+) and Na+, and Hill coefficients evaluated throughout the trial for Na+-ATPase were generally not affected by the treatments and habitat change. In comparison with the control group, in both treated groups, Na+-ATPase activity was lower during the freshwater phase and higher after brackish-water transfer. As compared with untreated trout, gill (Na++K+)-ATPase activity during the freshwater phase was stimulated by NaCl treatment and also by T(3) treatment after transfer to brackish water. The results indicate that NaCl and T(3) administration act differently on the two ATPase activities involved in Na+ regulation and suggest a prevalent role of Na+-ATPase activity in hypoosmotic conditions.
- Published
- 2001
- Full Text
- View/download PDF
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