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Objective: Large stimulus and myogenic artifacts usually prevent detection of sensory evoked potentials to electrical stimulation in trigeminal sensory territory (t-SEP). Stimulus Artifact (SA) removal can be obtained by means of two stimulating modes (Dual Mode Stimulation - DMS) having in common a fixed cathode alternatingly referred to opposed anodes, resulting in SAs of opposite polarity. Opposite SAs progressively cancel each other out during averaging, without interaction with the underlying bio-electrical events., Methods: Using DMS, dermatomal t-SEP were recorded from C5/C6 scalp sites in 24 healthy volunteers after selective, electrical stimulation of five trigeminal nerve areas: supraorbital, infraorbital, superior alveolar, inferior alveolar and auriculotemporal., Results: Reproducible t-SEPs were obtained after stimulation at all sites and showed the classical W shape, without significant differences related to the stimulated area. Cortical responses were formed by a sequence of individual peaks labelled, according to polarity and mean latency, as P8, N13, P19, N27, P38. A later, less stable component followed (N55-P67), poorly defined or absent in about one third of subjects., Conclusions: The described technique represents a novel approach, within reach of any neurophysiological unit, to record dermatomal SEPs to electrical stimulation of several, discrete areas of significant clinical interest, covering the whole trigeminal sensory territory., Significance: DMS represents a simple and robust tool to remove SA as the main drawback that has so far prevented recording of t-SEPs in daily clinical practice., Competing Interests: The Author has no competing interests to declare., (© 2022 International Federation of Clinical Neurophysiology. Published by Elsevier B.V.)

Objective: Intertrial variability (ITV) of motor responses to peripheral (CMAP) and transcranial (MEP) stimulation prevents their use in follow-up studies. Our purpose was to develop strategies to reduce and measure CMAP and MEP ITV to guide long-term monitoring of conduction slowing and conduction failure of peripheral and central motor pathway in the individual patient., Methods: Maximal compound muscle action potentials to High Voltage Electrical Stimulation (HVES) of lumbo-sacral nerve roots (r-CMAP) and activated, averaged motor evoked potentials (MEPs) to Transcranial Magnetic Stimulation (TMS) using double cone coil were recorded from 10 proximal and distal muscle districts of lower limbs. The procedure was repeated twice, 1-2 days apart, in 30 subjects, including healthy volunteers and clinically stable multiple sclerosis patients, using constant stimulating and recording sites and adopting a standardized procedure of voluntary activation. ITV for latency and area indexes and for the ratio between MEP and r-CMAP areas (a-Ratio) was expressed as Relative Intertrial Variation (RIV, 5th-95th percentile). As an inverse correlation between the size of area and ITV was found, raw ITV values were normalized as a function of area to make them comparable with one another., Results: All RIV values for latencies were significantly below the optimum threshold of ± 10%, with the exception of r-CMAP latencies recorded from Vastus Lateralis muscle. RIVs for a-Ratio, the most important index of central conduction failure, ranged from a maximum of -25.3% to +32.2% (Vastus Medialis) to a minimum of -15.0% to + 17.4% (Flexor Hallucis Brevis)., Conclusions: The described procedure represents an effort to lower as much as possible variability of motor responses in serial recording; the reported ITV normative values are the necessary premise to detect significant changes of motor conduction slowing and failure in the individual patient in follow-up studies.

Objective: The aim of this study was to investigate whether Transcranial Magnetic Stimulation (TMS) applied over the medial line of the scalp affects the subjective perception of continuous pain induced by means of electric stimulation. In addition, we wanted to identify the point of stimulation where this effect was maximum., Methods: Superficial electrical stimulation was used to induce continuous pain on the dominant hand. At the beginning of the experiment we reached a pain rating of 5 on an 11-point numeric rating scale (NRS; 0 = no pain and 10 = maximum tolerable pain) for each subject by setting individually the current intensity. The TMS (five pulses at increasing intensities) was applied on 5 equidistant points (one per session) over the medial line of the scalp in 13 healthy volunteers using a double-cone coil to stimulate underlying parts of the brain cortex. In every experimental session the painful stimulation lasted 45 minutes, during which pain and distress intensities NRS were recorded continuously. We calculated the effect of adaptation and the immediate effect of the TMS stimulation for all locations. Additionally, an ALE (Activation Likelihood Estimation) meta-analysis was performed to compare our results with the neuroimaging literature on subjective pain rating., Results: TMS stimulation temporarily decreased the pain ratings, and pain adaptation was suppressed when applying the TMS over the FCz site on the scalp. No effect was found for distress ratings., Conclusions: The present data suggest that the medial cortex in proximity of the cingulated gyrus has a causal role in adaptation mechanisms and in processing ongoing pain and subjective sensation of pain intensity.