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The worldwide spread of coronavirus COVID-19 infections demonstrates the great need for personal protective equipment and, in particular, hygiene masks. These masks are essential for the primary protection of the respiratory tract against pathogens such as viruses and bacteria that are infectious and transmitted through the air as large droplets or via small airborne particles. The use of protective masks will continue to accompany humans for an indefinite period of time, and therefore there is an urgent need for a safe method to extend their usability by reusing them under perspective with minimal loss of protective properties. Nanofiber mats are widely used in masks and in this study the reusability of nanofiber mats is investigated by washing them at different temperatures. This paper shows the first measurements of the washability of nanofiber mats. Furthermore, the air permeability is measured, and the evaporation resistance is evaluated. According to the results of this study, the air permeability performance of nanofiber mats does not change significantly after washing, confirming the possibility of reuse.