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Your search keyword '"Tavassoli, Marjan"' showing total 10 results
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10 results on '"Tavassoli, Marjan"'

1. Abstract P1183: Phosphorylation Signatures Of Human Heart Failure: Characterizing Dilated Cardiomyopathy-associated Signaling Pathways At The Cardiomyocyte Intercalated Disc

Author

Reitz, Cristine J, primary, Tavassoli, Marjan, additional, Kim, Da Hye, additional, Shah, Saumya, additional, Lakin, Robert, additional, Teng, Allen, additional, Zhou, Yu-Qing, additional, Li, Wenping, additional, Hadipour-Lakmehsari, Sina, additional, Backx, Peter, additional, Emili, Andrew, additional, Oudit, Gavin Y, additional, Kuzmanov, Uros, additional, and Gramolini, Anthony, additional

Published
2023
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2. Proteomics and phosphoproteomics of failing human left ventricle identifies dilated cardiomyopathy-associated phosphorylation of CTNNA3

Author

Reitz, Cristine J., primary, Tavassoli, Marjan, additional, Kim, Da Hye, additional, Shah, Saumya, additional, Lakin, Robert, additional, Teng, Allen C. T., additional, Zhou, Yu-Qing, additional, Li, Wenping, additional, Hadipour-Lakmehsari, Sina, additional, Backx, Peter H., additional, Emili, Andrew, additional, Oudit, Gavin Y., additional, Kuzmanov, Uros, additional, and Gramolini, Anthony O., additional

Published
2023
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3. An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease

Author

Teng, Allen C.T., primary, Tavassoli, Marjan, additional, Shrestha, Suja, additional, Marks, Ryan M., additional, McFadden, Meghan J., additional, Evagelou, Sonia L., additional, Lindsay, Kyle, additional, Vandenbelt, Ava, additional, Li, Wenping, additional, Ivakine, Evgueni, additional, Cohn, Ronald, additional, Santerre, J. Paul, additional, and Gramolini, Anthony O., additional

Published
2023
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4. Abstract MP216: Identification Of Novel Phosphoprotein Signaling Pathways In Human Dilated Cardiomyopathy By Integrative Proteomic And Phosphoproteomic Analysis

Author

Reitz, Cristine J, primary, Tavassoli, Marjan, additional, Kim, Da Hye, additional, Hadipour-Lakmehsari, Sina, additional, Shah, Saumya, additional, Lee, Shin-Haw, additional, Teng, Allen C, additional, Emili, Andrew, additional, Oudit, Gavin Y, additional, Kuzmanov, Uros, additional, and Gramolini, Anthony O, additional

Published
2021
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9. Fluorescence studies of nucleotide binding processes of AnmK (1,6-Anhydro-N-acetylmuramic acid kinase)

Author

McKenna, Sean (Chemistry) Mark, Brian (Microbiology), Khajehpour, Mazdak (Chemistry), Tavassoli, Marjan, McKenna, Sean (Chemistry) Mark, Brian (Microbiology), Khajehpour, Mazdak (Chemistry), and Tavassoli, Marjan

Abstract

1,6-Anhydro-N-acetylmuramic acid kinase (AnmK) is a homodimeric enzyme that can convert 1,6-Anhydro-N-acetylmuramic acid into N-acetylglucosamine-6-phosphate by consuming one molecule of ATP in murein recycling in Gram-negative bacteria. Structural data indicate that each subunit of AnmK is comprised of two domains that are separated by a deep active site cleft, which bears similarity to the ATPase core of proteins belonging to the hexokinase-hsp70-actin superfamily of proteins. These data also show that binding of ATP analogue changes the structure of AnmK. Our data suggest that AnmK is an allosteric enzyme and ATP binding follows Monod-Wyman-Changeux model (MWC). The apo-AnmK has two different conformations, one is more open (R state) and the other one is closed (T state). Binding of ATP to AnmK stabilizes the more open (R state) and makes AnmK prone to bind to anhMurNAc sugar.

Published
2013

10. An efficient and cost-effective purification protocol for Staphylococcus aureusCas9 nuclease

Author

Teng, Allen C.T., Tavassoli, Marjan, Shrestha, Suja, Marks, Ryan M., McFadden, Meghan J., Evagelou, Sonia L., Lindsay, Kyle, Vandenbelt, Ava, Li, Wenping, Ivakine, Evgueni, Cohn, Ronald, Santerre, J. Paul, and Gramolini, Anthony O.

Abstract

Here, we describe a protocol for purifying functional clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) from Staphylococcus aureuswithin 24 h and over 90% purity. SaCas9 purification begins with immobilized metal affinity chromatography, followed by cation exchange chromatography, and ended with centrifugal concentrators. The simplicity, cost-effectiveness, and reproducibility of such protocols will enable general labs to produce a sizable amount of Cas9 proteins, further accelerating CRISPR research.

Published
2022
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