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Your search keyword '"Takahara, Yoshiyuki"' showing total 27 results
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27 results on '"Takahara, Yoshiyuki"'

2. Identification and cloning of an Erwina carotovora subsp. carotovora bacteriocin regulator gene by insertional mutagenesis

Author

Chuang, Duen-Yau, Kyeremeh, Ampaabeng G., Gunji, Yuichi, Takahara, Yoshiyuki, Ehara, Yoshio, and Kikumoto, Toshio

Subjects
Bacteria, Phytopathogenic -- Genetic aspects, Bacterial toxins -- Genetic aspects, Biological sciences
Abstract

DNA containing the bacteriocin regulator gene (brg) from the phytopathogenic bacterium Erwinia carotovora subsp. carotovora CGE234M403's rifampin-resistant mutant, M-rif-11-2 were cloned and sequenced. One complete open reading frame (ORF), called ORF2, was found within the sequenced fragment. The 3' end of another ORF, ORF1, was found upstream of ORF2. A noncoding region and a putative promoter were found between ORF1 and ORF2.

Published
1999

11. Nucleotide Sequences and Organization of the Genes for Carotovoricin (Ctv) from Erwinia carotovora Indicate That Ctv Evolved from the Same Ancestor as Salmonella typhi Prophage.

Author

Yamada, Kazuteru, Hirota, Morihiko, Niimi, Yoshiko, Nguyen, Hoa Anh, Takahara, Yoshiyuki, Kami, Yoshiyuki, and Kaneko, Jun

Subjects
ERWINIA carotovora, GENES, BACTERIOPHAGES, NUCLEOTIDE sequence, BIOCHEMISTRY, BIOTECHNOLOGY
Abstract

The article focuses on the study conducted in Japan regarding the evolution of nucleotide sequences and organization of genes for Carotovocin from Erwinia carotovora. The authors discuss the gene organization and deduced amino acid sequences of carotovoricins in the chromosome on Salmonella enterica. Moreover, they talk about the mosaic structure of CtvEr genes and Mu-like prophage genes.

Published
2006
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13. Nucleotide Sequences and Organization of the Genes for Carotovoricin (Ctv) from Erwinia carotovoraIndicate That Ctv Evolved from the Same Ancestor as Salmonella typhiProphage

Author

YAMADA, Kazuteru, HIROTA, Morihiko, NIIMI, Yoshiko, NGUYEN, Hoa Anh, TAKAHARA, Yoshiyuki, KAMIO, Yoshiyuki, and KANEKO, Jun

Abstract

Carotovoricin Er (CtvEr), which is produced by a plant soft rot disease causative agent, Erwinia carotovorasubsp. carotovoraEr, is a high-molecular-weight bacteriocin showing Myoviridae phage-tail-like morphology with contractile sheath and plural tail fibers. We determined the complete nucleotide sequences of CtvEr genes on the E. carotovoraEr chromosome and report that CtvEr genes consist of lysis cassette, major and minor structural protein gene clusters. Four promoters were identified. The lysis gene cassette, which is composed of the genes for lysis enzyme and holin, was also identified and characterized. The nucleotide sequences and organization of the genes for CtvCGE, which is produced by E. carotovorastrain CGE234-M403 with the morphology similar to CtvEr, were also determined and compared to that of CtvEr, and it was found that CtvCGE is almost identical to CtvEr except for tail fibers which are involved in the killing spectra of both bacteriocins. We also explain that the gene organization and the deduced amino acid sequences of both carotovoricins are very close to those of prophage, which is lysogenized in the chromosome on Salmonella entericaserovar Typhi CT18. These findings strongly suggest that Ctv evolved as a phage tail-like bacteriocin from a common ancestor with Salmonella typhiprophage.

Published
2006
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14. Purification of enzymatically active poliovirus proteinase 3C produced in Escherichia coli

Author

Kondo Kiyosi, Kohara Michinori, Takahara Yoshiyuki, Hagino-Yamagishi Kimiko, Itoh Heihachi, Nomoto Akio, Ando Noriko, and Numao Naganori

Subjects
Viral protein, medicine.drug_class, viruses, Immunoblotting, Biology, medicine.disease_cause, Monoclonal antibody, law.invention, Viral Proteins, Capsid, Transformation, Genetic, law, Genetics, medicine, Escherichia coli, Cloning, Molecular, Protein Precursors, Gel electrophoresis, Chromatography, Poliovirus, Viral protein processing, 3C Viral Proteases, virus diseases, General Medicine, biochemical phenomena, metabolism, and nutrition, Molecular biology, Cysteine Endopeptidases, DNA, Viral, Recombinant DNA, Electrophoresis, Polyacrylamide Gel, Protein Processing, Post-Translational, Plasmids
Abstract

A viral protein 3C of the poliovirus (PV) Sabin 2 strain, a possible core region of the viral proteinase, was expressed in Escherichia coli using a recombinant DNA technology. The protein was recovered as a soluble protein from the insoluble protein fraction of the bacterial lysate, and was purified by a simple procedure with column chromatography. The viral capsid precursor P1 (1ABCD) of the PV Sabin 3 strain, which had been similarly produced in E. coli, was mixed with the purified or crude recombinant 3C. Immunoblotting assay with monoclonal antibodies specific to capsid proteins 1C (VP3) and 1D (VP1) of the PV Sabin 3 strain revealed that the in vitro reaction products contained 1C (VP3), 1D (VP1) and 1ABC (VP0–VP3). The data indicated that processing of the polyprotein P l by the recombinant 3C proceeded properly in vitro, although an undigested product, 1ABC, is always detected in the reaction mixture. The results strongly suggest that, in addition to a protein 3CD, the 3C protein itself is also catalytically active in the processing of the viral capsid precursor polyprotein P1.

Published
1989

27. Replicated studentized-deviate detection applied to the identification of differentially expressed genes in TNF-alpha-stimulated cells.

Author

Zhang QW, Ono N, Takahara Y, and Tanaka H

Subjects
Algorithms, Animals, Cell Line, Tumor, Models, Genetic, Reproducibility of Results, Gene Expression Profiling methods, Gene Expression Regulation drug effects, Oligonucleotide Array Sequence Analysis standards, Tumor Necrosis Factor-alpha pharmacology
Abstract

Among the applications of DNA microarray expression data, identification of differentially expressed genes is widely used to search for genes involved in specific biological processes. Previous studies have shown that replication is necessary to reliably identify differentially expressed genes. In practice, however, further guidelines are required to provide references for experiment design, that is, to fulfill the needs for determining the replication number on a desired confidence level or the detection sensitivity of the microarrays. Here, we performed simulations to build a simple statistical table of general guidelines adapted specifically for identifying differentially expressed genes using replications of a microarray experiment, and we present an accompanying nonparametric method, replicated studentized-deviate detection (RSD). The analysis results of the TNF-alpha-stimulated cells showed that RSD is simple and robust, and that the resulting statistical table is plausible.

Published
2004
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