1. Savitreea pentosicarens gen. nov., sp. nov., a yeast species in the family Saccharomycetaceae isolated from a grease trap
- Author
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Nantana Srisuk, Cécile Grondin, Chanita Boonmak, Noémie Jacques, Varunya Sakpuntoon, Serge Casaregola, Pannida Khunnamwong, Jirameth Angchuan, Kasetsart University - KU (THAILAND), Kasetsart University (KU), BIOlogie et GEstion des Risques en agriculture (BIOGER), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), TRF Research-Team Promotion Grant (RTA608004), International SciKU Branding (ISB), la Faculté des sciences, l’Université Kasetsart et l’UGSAS-GU via le «Microbiology Laboratory Station for IC - GU12» à Kasetsart, and Royal Golden Jubilee PhD programme PHD/0070/2560
- Subjects
0106 biological sciences ,[SDV]Life Sciences [q-bio] ,ascomycetous yeast ,novel yeast genus ,010603 evolutionary biology ,01 natural sciences ,Microbiology ,03 medical and health sciences ,novel yeast species ,grease trap ,Internal transcribed spacer ,Gene ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Genetics ,0303 health sciences ,biology ,Phylogenetic tree ,MycoBank ,Holotype ,General Medicine ,Ribosomal RNA ,biology.organism_classification ,Yeast ,Saccharomycetaceae ,[SDE]Environmental Sciences - Abstract
Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1/D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, Savitreea pentosicarens gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).
- Published
- 2020
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