Search

Your search keyword '"Szewc, Lukasz"' showing total 10 results
Start Over Author "Szewc, Lukasz" Search Limiters Full Text
10 results on '"Szewc, Lukasz"'

5. mRNA adenosine methylase (MTA) deposits m 6 A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana

Author

Bhat, Susheel Sagar, primary, Bielewicz, Dawid, additional, Gulanicz, Tomasz, additional, Bodi, Zsuzsanna, additional, Yu, Xiang, additional, Anderson, Stephen J., additional, Szewc, Lukasz, additional, Bajczyk, Mateusz, additional, Dolata, Jakub, additional, Grzelak, Natalia, additional, Smolinski, Dariusz J., additional, Gregory, Brian D., additional, Fray, Rupert G., additional, Jarmolowski, Artur, additional, and Szweykowska-Kulinska, Zofia, additional

Published
2020
Full Text
View/download PDF

9. mRNA adenosine methylase (MTA) deposits m6A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana.

Author

Bhat, Susheel Sagar, Bielewicz, Dawid, Gulanicz, Tomasz, Bodi, Zsuzsanna, Xiang Yu, Anderson, Stephen J., Szewc, Lukasz, Bajczyk, Mateusz, Dolata, Jakub, Grzelak, Natalia, Smolinski, Dariusz J., Gregory, Brian D., Fray, Rupert G., Jarmolowski, Artur, and Szweykowska-Kulinska, Zofia

Subjects
ARABIDOPSIS thaliana, RNA polymerase II, MICRORNA, PLANT RNA, NON-coding RNA
Abstract

In Arabidopsis thaliana, the METTL3 homolog, mRNA adenosine methylase (MTA) introduces N6-methyladenosine (m6A) into various coding and noncoding RNAs of the plant transcriptome. Here, we show that an MTA-deficient mutant (mta) has decreased levels of microRNAs (miRNAs) but accumulates primary miRNA transcripts (pri-miRNAs). Moreover, pri-miRNAs are methylated by MTA, and RNA structure probing analysis reveals a decrease in secondary structure within stem-loop regions of these transcripts in mta mutant plants. We demonstrate interaction between MTA and both RNA Polymerase II and TOUGH (TGH), a plant protein needed for early steps of miRNA biogenesis. Both MTA and TGH are necessary for efficient colocalization of the Microprocessor components Dicer-like 1 (DCL1) and Hyponastic Leaves 1 (HYL1) with RNA Polymerase II. We propose that secondary structure of miRNA precursors induced by their MTA-dependent m6A methylation status, together with direct interactions between MTA and TGH, influence the recruitment of Microprocessor to plant pri-miRNAs. Therefore, the lack of MTA in mta mutant plants disturbs pri-miRNA processing and leads to the decrease in miRNA accumulation. Furthermore, our findings reveal that reduced miR393b levels likely contributes to the impaired auxin response phenotypes of mta mutant plants. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results