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Olea pollen concentrations have been studied in relation to the typology of air masses, pollen grain sources and marine nature during advections in a coastal enclave in the south-eastern Iberian Peninsula. Since Spain is the world's leading olive producer, and olive growing extends throughout the Mediterranean basin, this location is ideal for the study of long-distance transport events (LTD) during the main pollen season (MPS). The air masses were classified using the calculation of 48-h back trajectories at 250, 500 and 750 m above ground level using the HYSPLIT model. After that, the frequency of LDT events from Africa and Europe was found to be 8.7% of the MPS days. In contrast, regional air masses were found in 38.6% of the MPS days. This was reflected in pollen concentrations, with significantly higher concentrations (p-value

Airborne particulate matter such as mineral dust comes mainly from natural sources, and the African regions of Sahara and Sahel originate large amounts of the aerosols dispersed worldwide. There is little knowledge about the influence of dust episodes on airborne pollen concentrations, and although the centre and southeast of the Iberian Peninsula are frequently affected by dust intrusions, until now, no specific works have analysed the effect of these episodes on airborne pollen concentrations in these areas. The aims of this study were to analyse the simultaneous occurrence of airborne pollen peaks and Saharan-Sahel dust intrusions in the central and south-eastern Iberian Peninsula, and to study the weather conditions – air mass pathways and conditions of air temperature, relative humidity and atmospheric pressure – that influence the airborne pollen concentrations during dust episodes. The results showed that the rise in airborne pollen concentrations during dust episodes is apparent in inland Iberian areas, although not in coastal areas in the southeast where pollen concentrations are even observed to decrease, coinciding with prevailing easterly winds from the sea. Total pollen concentrations and specific pollen types such as Olea, Poaceae and Quercus showed an increase in the central Iberian Peninsula during dust episodes when two meteorological phenomena concur: 1) prevailing winds from extensive areas of major wind-pollinated pollen sources over a medium or short distance (mainly from western and southwestern areas); and 2) optimal meteorological conditions that favour pollen release and dispersal into the atmosphere (mainly high temperatures and subsequently low humidity in central areas). Both conditions often occur during the Saharan-Sahel dust intrusions in the centre. Maximum pollen peaks are therefore most likely to occur during dust episodes in the central Iberian Peninsula, thus dramatically increasing the risk of outbreaks of pollinosis and other respiratory diseases in the population.

The Olea pollen is currently an important allergy source. In some regions of Southern Spain, olive pollen is the main cause of allergic sensitization exceeding 40% of the sensitized individuals. Due to the scarce presence of olive trees in Northern Spain, limited to some cultivated fields in the South of the Galicia region where they also grow wild, only 8% of the sensitized individuals showed positive results for Olea pollen. The aim of the paper was to assess the behaviour pattern of the Olea pollen and its aeroallergens in the atmosphere, as this information could help us to improve the understanding and prevention of clinical symptoms. Airborne Olea pollen and Ole e 1 allergens were quantified in Cartagena (South-eastern Spain) and Ourense (North-western Spain). A volumetric pollen trap and a Burkard Cyclone sampler were used for pollen and allergen quantification. The Olea flowering took place in April or May in both biometeorological sampling areas. The higher concentrations were registered in the Southern area of Spain, for both pollen and Ole e 1, with values 8 times higher for pollen concentrations and 40 times higher for allergens. An alternate bearing pattern could be observed, characterized by years with high pollen values and low allergen concentrations and vice versa. Moreover, during some flowering seasons the allergen concentrations did not correspond to the atmospheric pollen values. Variations in weather conditions or Long Distance Transport (LDT) processes could explain the discordance. The back trajectory analysis shows that the most important contributions of pollen and allergens in the atmosphere are coincident with air masses passing through potential source areas. The exposure to olive pollen may not be synonym of antigen exposure.

The incidence of pollinosis in developed countries has been on the rise for the past few decades. It is an illness that leads to a large number of missed work and school days. Therefore, in addition to the direct costs associated therewith, it also causes significant indirect costs. Avoidance to exposure is the most effective preventive measure. With aerobiological data for homogeneous geographical areas, sick people can both take preventive measures and optimize the period of usage of drugs and thus improve the quality of their life. Aerobiological networks have to decide how to provide this information. In the case of information intended for the general public, it is more useful to give it as concentration ranges adjusted to categories of low, medium and high/very high threshold values than as specific numerical values, which might not achieve the desired goal.In this paper we have analyzed the data generated by the Aerobiological Network of the Region of Murcia, Spain, from aerobiological databases in the 2010-2016 period. Based on the analysis of the 90, 95, 97 and 99 percentiles of the time series, we have proposed threshold values —in four levels (low, medium, high and very high), for the Poaceae and Urticaceae pollen types in the towns of Cartagena, Lorca and Murcia— that enhance the information supplied by said Aerobiological Network and are aimed at allowing allergic people to take avoidance and pharmacological treatment measures. La prevalencia de la polinosis está aumentando en los países desarrollados en las últimas décadas, siendo una enfermedad que ocasiona un gran número de días de pérdida de actividad laboral o de asistencia a la escuela, por lo que, además de los costes directos ligados a la enfermedad, ocasiona importantes costes indirectos. La evitación de la exposición es la medida preventiva más eficaz. El disponer de datos aerobiológicos para áreas geográficas homogéneas permite a los enfermos, tanto poner en marcha medidas preventivas, como optimizar el periodo de uso de medicamentos y mejorar la calidad de vida. Las redes aerobiológicas deben decidir el modo en que suministra la información. En el caso de información destinada a la población general, es más útil difundirla como intervalos de concentraciones ajustados a categorías de valores umbrales bajos, medios, altos o muy altos, que a un dato numérico concreto que puede no alcanzar el objetivo perseguido.En este trabajo hemos analizado esta información para los datos generados en la Red Aerobiológica de la Región de Murcia y a partir de las bases de datos aerobiológicas del periodo 2010-2016 hemos propuesto, basado en el análisis de los percentiles 90, 95, 97 y 99 de la serie temporal, unos valores umbrales, en cuatro escalones, bajo, medio, alto y muy alto para los tipos polínicos Poaceae y Urticaceae en la ciudades de Cartagena, Murcia y Lorca, que mejoran la información suministrada por la Red Aerobiológica de la Región de Murcia encaminada a que los pacientes alérgicos emprendan las medidas de evitación y el tratamiento farmacológico. A prevalência da polinose tem vindo a aumentar nos países desenvolvidos nas últimas décadas, sendo uma doença que ocasiona um grande número de dias de absentismo laboral ou escolar, pelo que, além dos custos diretos associados à doença, determina consideráveis custos indiretos. Evitar a exposição é a medida preventiva mais eficaz. Dispor de dados aerobiológicos para áreas geográficas homogéneas permite aos doentes quer adotar medidas preventivas, quer otimizar o período do uso de medicamentos, melhorando a sua qualidade de vida. As redes aerobiológicas devem decidir a forma como disponibilizam a informação. No caso de informação destinada à população em geral é mais útil difundi-la como intervalos de concentrações ajustados a categorias de valores limite baixos, médios, altos ou muito altos, considerando que um dado numérico concreto pode não atingir o objetivo pretendido.Neste trabalho foi analisada esta informação para os dados gerados na Rede Aerobiológica da Região de Múrcia, já a partir das bases de dados aerobiológicas no período 2010-2016. Baseados na análise dos percentis 90, 95, 97 e 99 da série temporal, foram propostos valores limites, em quatro escalões (baixo, médio, alto e muito alto), para os tipos polínicos Poaceae e Urticaceae, nas cidades de Cartagena, Múcia e Lorca, visando melhorar a informação disponibilizada pela Rede Aerobiológica da Região de Múrcia, destinada a que os pacientes alérgicos empreendam medidas de redução da exposição e o tratamento farmacológico.

There is a generalized consensus about the need to know the local aeropalynology in order to be able to properly understand pollinosis and make a correct etiological diagnosis. On the other hand, the aerobiological data provided by monitoring networks allow effective measures to be taken to prevent exposure.The aeropalynological characterization of the atmospheric aerosol is traditionally carried out by sampling by volume the particles suspended in the atmosphere and subsequently counting the pollen and fungus types present in the samples. One of the most commonly used sampling methods is based on the one proposed by Hirst in 1952. The correct identification of the pollen and fungus types present in the samples requires an important, previous education and training so as to be able to recognize the morphological features that would lead to a correct identification of the former.In the last decades of the 20th century data began to appear in scientific publications that drew the interest of researchers to the quantification of pollen allergens. In addition, the lack of a link between pollen grain counts and pollinosis symptoms (rhinitis, conjunctivitis and asthma) has been pointed out. On the other hand, there is controversy around the size of pollen grains and their likelihood to penetrate deeply into the respiratory tract. All this evidence led to the introduction of the concept of allergen load and the need to quantify it in the atmospheric aerosol.A lot of work has been done in this regard. In this paper we will supplement our experience in the subject matter with a review of the published literature in an attempt to determine whether we can answer the question posed by Beggs back in 1998: do we have to take pollen or pollen allergens into account? The analysis of the subject matter shows that there is not always a close correlation between pollen grain counts and the allergen load. In some cases, this behavior was expected and can be explained easily; in others, the reasons are not as clear. However, it has not been possible up until now to quantify all of the airborne allergens that are present in the bioaerosol. Thus, we are not ready to replace traditional aeropalynological counts yet and need to keep researching in this field to be able to develop a airborne allergen sampling and quantification methodology that can be implemented in aerobiological atmosphere monitoring networks. Based on the literature review we have conducted, we propose other possible avenues for aerobiological studies and voice our opinions about their future prospects. Existe un consenso generalizado sobre la necesidad de conocer la aeropalinología local para poder comprender de modo adecuado la polinosis y realizar un diagnóstico etiológico correcto. Por otro lado, los datos aerobiológicos suministrados por las redes de vigilancia permiten implantar medidas eficaces para evitar la exposición.La caracterización aeropalinológica del aerosol atmosférico se realiza tradicionalmente mediante el muestreo volumétrico de las partículas en suspensión en la atmósfera y posterior recuento de los tipos polínicos y fúngicos presentes en las muestras. Uno de los métodos de muestreo más utilizado se basa en el propuesto por Hirst en 1952. La correcta identificación de los tipos polínicos y fúngicos en las muestras requiere una formación altamente especializada, para poder reconocer las características morfológicas que permiten la adecuada adscripción a un tipo polínico o fúngico.A lo largo de las últimas décadas del siglo XX fueron apareciendo en publicaciones científicas datos que dirigieron el interés de los investigadores hacia la cuantificación de los alérgenos polínicos. Además, se ha señalado la falta de relación encontrada entre los recuentos de granos de polen y los síntomas de la polinosis (rinitis, conjuntivitis y el asma). Por otro lado, existe una controversia alrededor del tamaño de los granos de polen y su posibilidad de penetrar profundamente en las vías respiratorias. Este conjunto de evidencias propiciaron la introducción del concepto de carga alergénica y la necesidad de su cuantificación en el aerosol atmosférico.Son muchos los esfuerzos que se han realizado en este sentido, en este trabajo complementaremos nuestra experiencia en el tema con una revisión de la bibliografía publicada, tratando de examinar si se puede contestar a la pregunta formulada por Beggs ya en 1998; ¿qué hay que considerar el polen o los alérgenos polínicos?. El análisis del tema permite evidenciar que no siempre hay una correlación estrecha entre los recuentos de granos de polen y la carga alergénica, en algunos casos este comportamiento es fácilmente justificable y era previsible, en otros casos las razones no son tan evidentes. Sin embargo, hasta el momento no todos los aeroalérgenos han podido ser cuantificados en el bioaerosol, por lo que todavía no nos encontramos en condiciones de sustituir los recuentos aeropalinológicos tradicionales y se requiere seguir investigando en este campo para poder desarrollar una metodología de toma de muestras y cuantificación de aeroalérgenos que pueda ser implementada en las redes de vigilancia aerobiológica de la atmósfera. Siguiendo con la revisión bibliográfica realizada se apuntan otras opciones posibles para los estudios aerobiológicos y se recoge la opinión de los autores sobre sus posibilidades de futuro. Existe um consenso generalizado sobre a necessidade de conhecer a aeropalinologia local para poder compreender de modo adequado a polinose e realizar um diagnóstico etiológico correto. Por outro lado, os dados aerobiológicos fornecidos pelas redes de vigilância permitem implementar medidas eficazes para evitar a exposição.A caracterização aeropalinológica do aerossol atmosférico realiza-se tradicionalmente através da amostragem volumétrica de partículas em suspensão na atmosfera e posterior contagem dos tipos polínicos e fúngicos presentes nas amostras. Um dos métodos de amostragem mais utilizado baseia-se no proposto por Hirst em 1952. A correta identificação dos tipos polínicos e fúngicos nas amostras requer uma importante formação prévia, que permita reconhecer as caraterísticas morfológicas necessárias a uma correta identificação.Ao longo das últimas décadas do século XX foram surgindo em publicações científicas dados que direcionaram o interesse dos investigadores para a quantificação de alergénios polínicos. Além disso, assinalou-se a falta de relação encontrada entre as contagens de grãos de pólen e os sintomas de polinoses (rinites, conjuntivites e asma). Por outro lado, existe uma controvérsia em redor do tamanho dos grãos de pólen e a sua possibilidade de penetrar profundamente nas vias respiratórias. Este conjunto de evidências propiciou a introdução do conceito de carga alergénica e a necessidade da sua quantificação no aerossol atmosférico.São muitos os esforços que se realizaram nesse sentido, neste trabalho complementaremos a nossa experiência neste tema com uma revisão da bibliografia publicada, apreciando se é possível responder à pergunta formulada por Beggs já em 1998; “O que se deve considerar, o pólen ou os alergénicos polínicos?”. A análise do tema permite evidenciar que nem sempre há uma correlação estreita entre as contagens de grãos de pólen e a carga alergénica, em alguns casos esta situação é facilmente justificável e era previsível, em outros casos as razões não são tão evidentes. Porém, até ao momento nem todos os aeroalergénios podem ser quantificados no bioaerossol, pelo que não nos encontramos em condições de substituir as contagens aeropalinológicas tradicionais, sendo necessário dar continuidade à investigação neste campo no sentido de se desenvolver uma metodologia de colheita de amostras e quantificação de aeroalergénios que possam ser implementadas nas redes de vigilância aerobiológica da atmosfera. Em consequência da revisão bibliográfica realizada registam-se outras opções possíveis para os estudos aerobiológicos e recolhe-se a opinião de outros autores sobre as possibilidades futuras.

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African dust outbreaks over the Iberian Peninsula have been related to four synoptic patterns responsible for the advection of dust: 1. A North African high located at surface level. 2. An Atlantic depression centered over northwestern Africa, western Iberia or the southwest of the Portuguese coast with an associated high or ridge over the Mediterranean Sea. 3. A North African depression. 4. A North African high located at upper levels. Consequently, particulate matter (PM) levels in Iberia are expected to rise when any of these atmospheric synoptic scenarios prevail. Nevertheless, PM levels might not increase due to wet deposition, as Spain receives the most African-derived dust rain events of any European country. In this study, a meteorological scenario different than the above situations is evaluated.

Background: Associations found in time-series studies on hospital emergency room (ER) visits due to asthma and chronic obstructive pulmonary disease (COPD) with single air pollutants show some lack of consistency. The respiratory effects of aeroallergens in the air pollution mix are not well established. Non-linear relationships of different airborne pollen types with certain respiratory diseases have also been described. We aim to study the short-term effects of major air pollutants and aeroallergen pollen on asthma and COPD hospital ER visits in the industrial and Mediterranean Spanish city of Cartagena during 1995-1998. Methods: The association of asthma and COPD to ER visits with mean levels of sulphur and nitrogen dioxides (SO 2 and NO 2), total suspended particles (TSP), ozone (O 3), and the main allergenic airborne pollen types were analysed using Poisson regression with Generalised Additive Models, taking into account delayed effects and adjusting for long-term trends, seasonality, weather conditions, holidays and flu notifications. Results: Multipollutant models showed a similar relative risk (RR) increase (in %), of around 5% in asthma and COPD ER visits per 10μg/m 3 SO 2increments. The risk of an ER visit for the same NO 2 increment was 2.6% for asthma and 3.3% for COPD. Visits to the ER due to asthma showed a positive increase with both Urticaceae and Poaceae levels, but did not substantially modify the previous percentages. Conclusions: Air levels of SO 2 and NO 2 were associated with a substantial increased risk in ER visits due to asthma and COPD. The inclusion of Poaceae and Urticaceae pollen did not alter that association. © 2011 SEICAP., BACKGROUND Associations found in time-series studies on hospital emergency room (ER) visits due to asthma and chronic obstructive pulmonary disease (COPD) with single air pollutants show some lack of consistency. The respiratory effects of aeroallergens in the air pollution mix are not well established. Non-linear relationships of different airborne pollen types with certain respiratory diseases have also been described. We aim to study the short-term effects of major air pollutants and aeroallergen pollen on asthma and COPD hospital ER visits in the industrial and Mediterranean Spanish city of Cartagena during 1995-1998. METHODS The association of asthma and COPD to ER visits with mean levels of sulphur and nitrogen dioxides (SO(2) and NO(2)), total suspended particles (TSP), ozone (O(3)), and the main allergenic airborne pollen types were analysed using Poisson regression with Generalised Additive Models, taking into account delayed effects and adjusting for long-term trends, seasonality, weather conditions, holidays and flu notifications. RESULTS Multipollutant models showed a similar relative risk (RR) increase (in %), of around 5% in asthma and COPD ER visits per 10μg/m(3) SO(2)increments. The risk of an ER visit for the same NO(2) increment was 2.6% for asthma and 3.3% for COPD. Visits to the ER due to asthma showed a positive increase with both Urticaceae and Poaceae levels, but did not substantially modify the previous percentages. CONCLUSIONS Air levels of SO(2) and NO(2) were associated with a substantial increased risk in ER visits due to asthma and COPD. The inclusion of Poaceae and Urticaceae pollen did not alter that association.

Aerobiological monitoring of Olea europaea L. is of great interest in the Mediterranean basin because olive pollen is one of the most represented pollen types of the airborne spectrum for the Mediterranean region, and olive pollen is considered one of the major cause of pollinosis in this region. The main aim of this study was to develop an airborne-pollen map based on the Pollen Index across a 4-year period (2008-2011), to provide a continuous geographic map for pollen intensity that will have practical applications from the agronomical and allergological points of view. For this purpose, the main predictor variable was an index based on the distribution and abundance of potential sources of pollen emission, including intrinsic information about the general atmospheric patterns of pollen dispersal. In addition, meteorological variables were included in the modeling, together with spatial interpolation, to allow the definition of a spatial model of the Pollen Index from the main olive cultivation areas in the Mediterranean region. The results show marked differences with respect to the dispersal patterns associated to the altitudinal gradient. The findings indicate that areas located at an altitude above 300ma.s.l. receive greater amounts of olive pollen from shorter-distance pollen sources (maximum influence, 27km) with respect to areas lower than 300ma.s.l. (maximum influence, 59km).

Human semen quality may be declining due to environmental pollutants, occupational exposures or changes in lifestyle. Nonetheless, we still know little about the impact of those factors on male fertility. Some heavy metals, volatile organic compounds or xenoestrogens may compromise reproductive male function. This process could take place along the human life cycle and not only in certain stages of development. We review the main factors that affect human male fertility and their possible influence in current human reproduction.

Despite their significant role in source apportionment analysis, studies dedicated to the identification of tracer elements of emission sources of atmospheric particulate matter based on air quality data are relatively scarce. The studies describing tracer elements of specific sources currently available in the literature mostly focus on emissions from traffic or large-scale combustion processes (e.g. power plants), but not on specific industrial processes. Furthermore, marker elements are not usually determined at receptor sites, but during emission. In our study, trace element concentrations in PM10 and PM2.5 were determined at 33 monitoring stations in Spain throughout the period 1995–2006. Industrial emissions from different forms of metallurgy (steel, stainless steel, copper, zinc), ceramic and petrochemical industries were evaluated. Results obtained at sites with no significant industrial development allowed us to define usual concentration ranges for a number of trace elements in rural and urban background environments. At industrial and traffic hotspots, average trace metal concentrations were highest, exceeding rural background levels by even one order of magnitude in the cases of Cr, Mn, Cu, Zn, As, Sn, W, V, Ni, Cs and Pb. Steel production emissions were linked to high levels of Cr, Mn, Ni, Zn, Mo, Cd, Se and Sn (and probably Pb). Copper metallurgy areas showed high levels of As, Bi, Ga and Cu. Zinc metallurgy was characterised by high levels of Zn and Cd. Glazed ceramic production areas were linked to high levels of Zn, As, Se, Zr, Cs, Tl, Li, Co and Pb. High levels of Ni and V (in association) were tracers of petrochemical plants and/or fuel-oil combustion. At one site under the influence of heavy vessel traffic these elements could be considered tracers (although not exclusively) of shipping emissions. Levels of Zn–Ba and Cu–Sb were relatively high in urban areas when compared with industrialised regions due to tyre and brake abrasion, respectively.

Air quality is a major issue for humans owing to the fact that the content of particles in the atmosphere has multiple implications for life quality, ecosystem dynamics and environment. Scientists are therefore particularly interested in discovering the origin of airborne particles. A new method has been developed to model the relationship between the emission surface and the total amount of airborne particles at a given distance, employing olive pollen and olive groves as examples. A third-degree polynomial relationship between the air particles at a particular point and the distance from the source was observed, signifying that the nearest area to a point is not that which is most correlated with its air features. This work allows the origin of airborne particles to be discovered and could be implemented in different disciplines related to atmospheric aerosol, thus providing a new approach with which to discover the dynamics of airborne particles.

In the presented study the airborne fungal spores of the semiarid city of Cartagena, Spain, are identified and quantified by means of viable or non-viable sampling methods. Airborne fungal samples were collected simultaneously using a filtration method and a pollen and particle sampler based on the Hirst methodology. This information is very useful for elucidating geographical patterns of hay fever and asthma. The qualitative results showed that when the non-viable methodology was employed, Cladosporium, Ustilago, and Alternaria were the most abundant spores identified in the atmosphere of Cartagena, while the viable methodology showed that the most abundant taxa were: Cladosporium, Penicillium, Aspergillus and Alternaria. The quantitative results of airborne fungal spores identified by the Hirst-type air sampler (non-viable method), showed that Deuteromycetes represented 74% of total annual spore counts, Cladosporium being the major component of the fungal spectrum (62.2%), followed by Alternaria (5.3%), and Stemphylium (1.3%). The Basidiomycetes group represented 18.9% of total annual spore counts, Ustilago (7.1%) being the most representative taxon of this group and the second most abundant spore type. Ascomycetes accounted for 6.9%, Nectria (2.3%) being the principal taxon. Oomycetes (0.2%) and Zygomycestes and Myxomycestes (0.06%) were scarce. The prevailing species define our bioaerosol as typical of dry air. The viable methodology was better at identifying small hyaline spores and allowed for the discrimination of the genus of some spore types. However, non-viable methods revealed the richness of fungal types present in the bioaerosol. Thus, the use of both methodologies provides a more comprehensive characterization of the spore profile.

Total suspended particulate matter (TSP) and lead (Pb) concentrations were monitored at three sampling stations in Cartagena, Spain, from February 1990 to December 1998, and copper (Cu), zinc (Zn), and cadmium (Cd) atmospheric concentrations were measured from January 1991 to December 1998. TSP and Pb values were analysed from samples collected on glassfibre filters, and the concentrations of Pb, Cu, Zn, and Cd were calculated from cellulose ester filters, showing no statistically significant difference (95% C.L.) for Pb analysed in both filter types. The geographical and temporal distribution patterns were investigated. There was a trend to find the highest TSP levels during winter and autumn, and the highest Pb concentrations during the winter season. The Pb/Cd average ratios for each sampling point showed a direct anthropogenic contribution for the industrial areas, when compared with Pb/Cd ratio reported for global natural emissions. The correlation among heavy metals displayed similar results, differing from the residential area to the urban and industrial ones.

PURPOSE: The problems of air pollution became noticeable in Cartagena in the seventies, high SO2 and particle levels having been reached from time to time. Our aim is to assess, using the EMECAM methodology, the acute impact of SO2 and particle air pollution on the daily mortality of the city of Cartagena in the 1992-1996 period. METHODS: A daily listing is provided of the total number of non-accidental deaths within the population as a whole and for those over age 70, the cardiovascular and the respiratory deaths due to the sulfur dioxide and particle air pollution for the 1992-1996 period using autoregressive Poisson models which control seasonality, weather, time of year, flu, special events, and time lags. RESULTS: In the period under study, there has been a drop in the SO2 air pollution as compared to previous years, which was not as marked for the particles. The analyses reveal significant relationships in the total non-accidental deaths in those over age 69, with the average particle count and those particles with cardiovascular deaths for the months of May to October. In the six-month period of the year when the weather is cold, we found a positive statistically significant relationship to exist in the maximum daily hourly value of the particles and the deaths due to cardiocirculatory and respiratory diseases. However, there is no consistency in the between on assessing the reliability of the models.

It has been hypothesized that exposure to heavy metals may impair male reproduction. To measure the effect produced by low doses of heavy metals on semen parameters, it is necessary to clarify in which body fluids those measurements must be performed. Sixty-one men attending infertility clinics participated in our study. Concentrations of lead, cadmium, and mercury were measured in whole blood, blood plasma, and seminal plasma using spectroanalytical and electrochemical methods. Semen analyses were performed according to World Health Organization criteria. For statistical analysis, Spearman's rank correlations, mean comparison tests, and discriminant analysis were calculated. Significant correlations between the measured concentrations of the three heavy metals in the same biological fluids were observed. However, no similar relationship was seen when comparing the concentrations in different body fluids of the same metal. According to our results and previous publications, seminal plasma might be the best body fluid for assessing impairment of human semen parameters.

Background Animal studies have shown the reproductive toxicity of a number of heavy metals. Very few human observational studies have analyzed the relationship between male reproductive function and heavy metal concentrations in diverse biological fluids. Methods The current study assessed the associations between seminal and hormonal parameters and the concentration of the 3 most frequent heavy metal toxicants (lead, cadmium and mercury) in three different body fluids. Sixty one men attending infertility clinics that participated in a case-control study to explore the role of environmental toxins and lifestyles on male infertility were analyzed. Concentration of lead, cadmium and mercury were measured in blood and seminal plasma and whole blood using anodic stripping voltammetry and atomic absorption spectrophotometry. Serum samples were analyzed for follicle-stimulating hormone, luteinizing hormone and testosterone. Semen analyses were performed according to World Health Organization criteria. Mann-Whitney test and Spearman's rank correlations were used for unadjusted analyses. Multiple linear regression models were performed controlling for age, body mass index and number of cigarettes per day. Results There were no significant differences between cases and controls in the concentrations of heavy metals in any of the three body fluids. In multivariate analyses using all subjects no significant associations were found between serum hormone levels and metal concentrations. However there was a significant positive association between the percentage of immotile sperms and seminal plasma levels of lead and cadmium. Conclusions Our results suggest that the presence of lead and cadmium in the reproductive tract of men may be related to a moderate alteration of their seminal parameters.

Ten years of public health interventions on industrial emissions to clean air were monitored for the Mediterranean city of Cartagena. During the 1960s, a number of large chemical and non-ferrous metallurgical factories were established that significantly deteriorated the city's air quality. By the 1970s, the average annual air concentration of sulfur dioxide (SO2) ranged from 200 to 300 A mu g/m(3) (standard conditions units). In 1979, the Spanish government implemented an industrial intervention plan to improve the performance of factories and industrial air pollution surveillance. Unplanned urban development led to residential housing being located adjacent to three major factories. Factory A produced lead, factory B processed zinc from ore concentrates, and factory C produced sulfuric acid and phosphates. This, in combination with the particular abrupt topography and frequent atmospheric thermal inversions, resulted in the worsening of air quality and heightening concern for public health. In 1990, the City Council authorized the immediate intervention at these factories to reduce or shut down production if ambient levels of SO2 or total suspended particles (TSP) exceeded a time-emission threshold in pre-established meteorological contexts. The aim of this research was to assess the appropriateness and effectiveness of the intervention plan implemented from 1992 to 2001 to abate industrial air pollution. The maximum daily 1-h ambient air level of SO2, NO2, and TSP pollutants was selected from one of the three urban automatic stations, designed to monitor ambient air quality around industrial emissions sources. The day on which an intervention took place to reduce and/or interrupt industrial production by factory and pollutant was defined as a control day, and the day after an intervention as a post-control day. To assess the short-term intervention effect on air quality, an ecological time series design was applied, using regression analysis in generalized additive models, focusing on day-to-day variations of ambient air pollutants levels. Two indicators were estimated: (a) appropriateness, the ratio between mean levels of the pollutant for control days versus the other days, and (b) effectiveness, the ratio between mean levels of the pollutant for post-control days versus the other days. Ratios in regression analyses were adjusted for trend, seasonality, temperature, humidity and atmospheric pressure, calendar day, and special events as well as the other pollutants. A total of 702 control days were made on the factories' industrial production during the 10-year period. Fifteen reductions and five shutdown control days took place at factory A for ambient air SO2. At factory B, more controls were carried out for the SO2 pollutant in the years 1992-1993 and 1997. At factory C, the control days for SO2 decreased from 59 reductions and 14 shutdowns to a minimum from 1995 onwards, whereas the controls on TSP were more frequent, reaching a maximum of 99 reductions and 47 shutdowns in the last year. SO2 ambient air mean levels ranged from 456 to 699 A mu g/m(3) among factories on reduction control days and between 624 and 1,010 A mu g/m(3) on shutdown days. The TSP ambient air mean levels were 428 and 506 A mu g/m(3) on reduction and shutdown days, respectively. For all types of control days and factories, a mean ratio of 104% (95% confidence interval [CI] 88 to 121) in SO2 levels was obtained and a mean ratio of 67% (95% CI 59 to 75) in TSP levels. Post-control days at all factories showed a mean ratio of -16% (95% CI -7 to -24) in SO2 levels and a mean ratio of -13% (95% CI -7 to -19) in TSP levels. Interventions on industrial production based on the urban SO2 and TSP ambient air levels were justified by the high concentrations detected. The best assessment of the interventions' effectiveness would have been to utilize the ambient air pollutant concentration readings from the entire time of the production shutdowns or reductions; however, the daily hourly maximum turned out to be a useful indicator because of meteorological factors influencing the diurnal concentration profile. A substantial number of interventions were carried out from 1 to 3 am, when vehicular traffic was minimum. On the other hand, atmospheric stability undergoes diurnal cycling in the autumn-winter period due to thermal inversion, which reaches maximum levels around daybreak. Therefore, this increases the ambient air levels and justified the interventions carried out at daybreak in spite of the traffic influence. All the interventions for SO2 and TSP were carried out when the measured ambient air levels of pollutants were exceeded, which shows the appropriateness of the intervention program. This excess was greater when intervening on SO2 than on the TSP levels. For both ambient air levels of SO2 and TSP, significant drops in air pollution were achieved from all three factories following activity reductions. The production shutdown controls were very effective, because they returned excess levels, higher than in the reduction controls, to everyday mean values. The Cartagena City observational system of intermittent control has proven to effectively reduce industrial emissions' impact on ambient air quality. This experienced model approach could serve well in highly polluted industrial settings. From a public health perspective, studies are needed to assess that the industrial interventions to control air pollution were related to healthier human populations. Legislation was needed to allow the public administration to take direct actions upon the polluting industries.

Objective To compare specific nutrient intake between normospermic and oligoasthenoteratospermic patients attending infertility clinics in two Mediterranean provinces of Spain. Design Case-control study. Setting Private fertility clinics in southeastern Spain. Patient(s) Thirty men with poor semen quality (case subjects) and 31 normospermic control subjects of couples attending our fertility clinics. Intervention(s) We recorded dietary habits and nutrient consumption using a food frequency questionnaire adapted to meet specific study objectives. Main Outcome Measure(s) We calculated nutrient intakes by multiplying the frequency of use for each food by the nutrient composition of the portion size specified on the food frequency questionnaire and by addition across all foods to obtain a total nutrient intake for each individual. Semen quality was assessed by measuring volume, concentration, motility, and morphology. Hormones levels were also analyzed in case and control subjects. Result(s) In the logistic regression, control subjects had a significantly higher intake of carbohydrates, fiber, folate, vitamin C, and lycopene and lower intakes of proteins and total fat. Conclusion(s) A low intake of antioxidant nutrients was associated with a poor semen quality in this case-control study of Spanish men attending infertility clinics.

Average ranges of particulate matter (PM10 and PM2.5) concentrations and chemical composition in Spain show significant variations across the country, with current PM10 levels at several industrial and traffic hotspots exceeding recommended pollution limits. Such variations and exceedances are linked to patterns of anthropogenic and natural PM emissions, climate, and reactivity/stability of particulate species. PM10 and PM2.5 concentrations reach 14–22 μg PM10 m−3 and 8–12 μg PM2.5 m−3 at most rural/regional background sites, 25–30 μg PM10 m−3 and 15–20μg PM2.5 m−3 at suburban sites, 30–46 μg PM10 m−3 and 20–30 μg PM2.5 m−3 at urban background and industrial sites, and 46–50 μg PM10 m−3 and 30–35 μg PM2.5 m−3 at heavy traffic hotpots. Spatial distributions show sulphate and carbon particle levels reach maxima in industrialised areas and large cities (where traffic emissions are higher), and nitrate levels increase from the Atlantic to the Mediterranean (independent of the regional NOx emissions). African dust outbreaks have an influence on the number of exceedances of the daily limit value, but its additional load on the mean annual PM10 levels is only highly significant in Southern Iberia and Canary and Balearic islands. The marine aerosol contribution is near one order of magnitude higher in the Canaries compared to the other regions. Important temporal influences include PM intrusion events from Africa (more abundant in February–March and spring–summer), regional-scale pollution episodes, and weekday versus weekend activity. Higher summer insolation enhances (NH4)2SO4 but depletes particulate NO3− (as a consequence of the thermal instability of ammonium nitrate in summer) and Cl− (due to HCl volatilisation resulting from the interaction of gaseous HNO3 with the marine NaCl), as well as generally increasing dry dust resuspension under a semi-arid climate. Average trace metal concentrations rise with the highest levels at industrial and traffic hotspots sites, in some cases (Ti, Cr, Mn, Cu, Zn, As, Sn, W, and Pb) exceeding rural background levels by over an order of magnitude.

Objective To compare dietary habits in normospermic and oligoasthenoteratospermic patients attending a reproductive assisted clinic. Design An observational, analytical case-control study. Setting Private fertility clinics. Patient(s) Thirty men with poor semen quality (cases) and 31 normospermic control couples attending our fertility clinics. Intervention(s) We recorded dietary habits and food consumption using a food frequency questionnaire adapted to meet specific study objectives. Analysis of semen parameters, hormone levels, Y microdeletions, and karyotypes were also carried out. Main Outcome Measure(s) Frequency of intake food items were registered in a scale with nine categories ranging from no consumption to repeated daily consumption. Result(s) Controls had a higher intake of skimmed milk, shellfish, tomatoes, and lettuce, and cases consumed more yogurt, meat products, and potatoes. In the logistic regression model cases had lower intake of lettuce and tomatoes, fruits (apricots and peaches), and significantly higher intake of dairy and meat processed products. Conclusion(s) Frequent intake of lipophilic foods like meat products or milk may negatively affect semen quality in humans, whereas some fruits or vegetables may maintain or improve semen quality.

A method using differential pulse anodic stripping voltammetry after microwave oven digestion was developed for the simultaneous determination of Cd(II) and Pb(II) in the deciduous teeth of children. Each tooth was weighed; deposited in a 120 mL capped Teflon vessel with 5 mL 65% nitric acid, Suprapur analytical grade; and digested in a 2-step microwave oven for 15 min. The detection limits for Cd(II) and Pb(II) in the final solution were 0.078 and 0.323 μg/L, and the quantitation limits 0.394 and 1.613 μg/L, respectively, with a linearity range of 2 μg/L for Cd(II) and 23.3 μg/L for Pb(II). The sensitivity was 2.51 nA/μg-L and 1.37 nA/μg-L, for Cd(II) and Pb(II). The main advantages of this technique are a complete and satisfactory dissolution of the tooth material with the proposed microwave oven digestion procedure, without sample pretreatments, such as drying, ashing, or powdering. The voltammetric procedure proved to be well designed because of significant goodness of fit to a linear model, and the accuracy of the method was established as compared with standard reference material. The methodology has enabled us to study Cd(II) and Pb(II) in 371 deciduous teeth from school children in Cartagena, Spain.

Zygophyllum fabago L. (Zygophyllaceae) can be found in the Middle East, in North Africa and in the arid zones of the Mediterranean region. It easily establishes itself in new regions, and is considered an invasive plant. They undergo ambophilous pollination, as there is a relationship between this type of pollination and its allergenic incidence. A combination of transmission electron microscopy with immunocytochemical methods was used to localize allergenic proteins during hydration and activation processes. Germination was induced in vitro for 1,2,4,6, and 30 min. The activated proteins reacting with antibodies present in human sera from allergenic patients are found in the cytoplasm, intine, exine and exudates from the pollen grains. The activation time plays an important role on the labelling intensity. Labelling of allergenic proteins was abundant at 1 and 2 min of activation, and decreased at 4 and 6 min. The rapid activation and release of the allergenic proteins appears to be the main cause of allergenic activity of Z. fabago pollen grains.

The aim of this paper is to show the results of lead and cadmium values measured on deciduous teeth of children from the city of Cartagena (Spain), and their relationships with different variables. A total of 834 samples were processed. Lead data shown a positively skewed distribution and fitted to a lognormal distribution, meanwhile cadmium data did not. The true arithmetic mean and standard deviation of cadmium values was estimated censoring data by 10 nglg, where the cadmium distribution looks log-normal. No statistically significant differences could be observed for lead and cadmium values according to the sex of donor. However, the content of both heavy metals decreased from incisors to molars, and also with the age of shedding, although this last factor was not important when considering a multifactor ANOVA. The use of fluoride and the zone of residence proved to be the most important environmental variables affecting the content of lead and cadmium, meanwhile other parameters, i.e., home antiquity or family socio-economic status were significant for lead values.

This case–control study explored the role of environmental toxins in male infertility in patients attending an assisted reproduction clinic in southeastern Spain. Exposures were compared by questionnaire for 30 infertile oligoasthenoteratozoospermic males (cases) and 31 normozoospermic controls residing in the area. Odds ratios and 95% confidence intervals (CI) were used to estimate differences in lifestyle and chemical occupational exposures. More than two-thirds of the cases (23/30), compared with less than one-third of controls (10/31), had been exposed occupationally to at least one toxin or pollutant (OR = 6.9; 95% CI: 2.2–21.4) and were also more exposed to them currently (OR = 5.2; 95% CI: 1.6–17.2). Exposure to glues, solvents or silicones (OR = 22.9; 95% CI: 2.8–190.9), metals (OR = 8.8; 95% CI: 1.4–54.2) and physical agents (OR = 7.3; 95% CI: 1.4–36.7) in the past, as well as current exposure to glues, solvents or silicones (OR = 10.4; 95% CI: 2.6–42.5) and physical agents (OR = 4.7; 95% CI: 1.1–19.2), were significantly higher in cases than in controls. Average duration of exposure was also significantly higher in cases (P < 0.001). This study suggests that male infertility in patients attending infertility clinics may often be the result of occupational exposure.