4 results on '"Soraya Djerbi"'
Search Results
2. Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses
- Author
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Ewa J. Mellerowicz, Nobuyuki Nishikubo, Pedro M. Coutinho, Matt Geisler, Stanislaw Karpinski, Junko Takahashi, Björn Sundberg, Jane Geisler-Lee, Soraya Djerbi, Henrik Aspeborg, Sara Andersson-Gunnerås, Leszek A. Kleczkowski, Bo Segerman, Emma R. Master, Tuula T. Teeri, and Bernard Henrissat
- Subjects
Populus trichocarpa ,Expressed sequence tag ,biology ,Physiology ,fungi ,food and beverages ,Plant Science ,biology.organism_classification ,Biochemistry ,Arabidopsis ,Gene expression ,Glycosyltransferase ,Genetics ,biology.protein ,Sucrose synthase ,Glycoside hydrolase ,Gene - Abstract
Over 1,600 genes encoding carbohydrate-active enzymes (CAZymes) in the Populus trichocarpa (Torr. & Gray) genome were identified based on sequence homology, annotated, and grouped into families of glycosyltransferases, glycoside hydrolases, carbohydrate esterases, polysaccharide lyases, and expansins. Poplar (Populus spp.) had approximately 1.6 times more CAZyme genes than Arabidopsis (Arabidopsis thaliana). Whereas most families were proportionally increased, xylan and pectin-related families were underrepresented and the GT1 family of secondary metabolite-glycosylating enzymes was overrepresented in poplar. CAZyme gene expression in poplar was analyzed using a collection of 100,000 expressed sequence tags from 17 different tissues and compared to microarray data for poplar and Arabidopsis. Expression of genes involved in pectin and hemicellulose metabolism was detected in all tissues, indicating a constant maintenance of transcripts encoding enzymes remodeling the cell wall matrix. The most abundant transcripts encoded sucrose synthases that were specifically expressed in wood-forming tissues along with cellulose synthase and homologs of KORRIGAN and ELP1. Woody tissues were the richest source of various other CAZyme transcripts, demonstrating the importance of this group of enzymes for xylogenesis. In contrast, there was little expression of genes related to starch metabolism during wood formation, consistent with the preferential flux of carbon to cell wall biosynthesis. Seasonally dormant meristems of poplar showed a high prevalence of transcripts related to starch metabolism and surprisingly retained transcripts of some cell wall synthesis enzymes. The data showed profound changes in CAZyme transcriptomes in different poplar tissues and pointed to some key differences in CAZyme genes and their regulation between herbaceous and woody plants.
- Published
- 2006
3. The genome sequence of black cottonwood (Populus trichocarpa) reveals 18 conserved cellulose synthase (CesA) genes
- Author
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Mats Lindskog, Tuula T. Teeri, Lars Arvestad, Fredrik Sterky, and Soraya Djerbi
- Subjects
Expressed Sequence Tags ,Whole genome sequencing ,Genetics ,Populus trichocarpa ,DNA, Complementary ,Phylogenetic tree ,cDNA library ,Chromosome Mapping ,Plant Science ,Biology ,biology.organism_classification ,Genome ,Chromosomes, Plant ,Populus ,Glucosyltransferases ,Complementary DNA ,Gene expression ,Gene ,Conserved Sequence ,Genome, Plant ,Phylogeny - Abstract
The genome sequence of Populus trichocarpa was screened for genes encoding cellulose synthases by using full-length cDNA sequences and ESTs previously identified in the tissue specific cDNA libraries of other poplars. The data obtained revealed 18 distinct CesA gene sequences in P. trichocarpa. The identified genes were grouped in seven gene pairs, one group of three sequences and one single gene. Evidence from gene expression studies of hybrid aspen suggests that both copies of at least one pair, CesA3-1 and CesA3-2, are actively transcribed. No sequences corresponding to the gene pair, CesA6-1 and CesA6-2, were found in Arabidopsis or hybrid aspen, while one homologous gene has been identified in the rice genome and an active transcript in Populus tremuloides. A phylogenetic analysis suggests that the CesA genes previously associated with secondary cell wall synthesis originate from a single ancestor gene and group in three distinct subgroups. The newly identified copies of CesA genes in P. trichocarpa give rise to a number of new questions concerning the mechanism of cellulose synthesis in trees.
- Published
- 2005
4. Carbohydrate-Active Enzymes Involved in the Secondary Cell Wall Biogenesis in Hybrid Aspen
- Author
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Pedro M. Coutinho, Ewa J. Mellerowicz, Peter Nilsson, Mark Stam, Henrik Aspeborg, Bernard Henrissat, Björn Sundberg, Jarmo Schrader, Bahram Amini, Emma R. Master, Åsa M. Kallas, Soraya Djerbi, Fredrik Sterky, Göran Sandberg, Stuart E. Denman, and Tuula T. Teeri
- Subjects
chemistry.chemical_classification ,Expressed sequence tag ,Physiology ,Plant Science ,Biology ,Xylan acetylation ,Enzyme ,chemistry ,Biochemistry ,Complementary DNA ,Glycosyltransferase ,Genetics ,biology.protein ,Glycoside hydrolase ,DNA microarray ,Functional genomics - Abstract
Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis is currently relatively well understood, the pathways leading to the synthesis of the key structural carbohydrates in wood fibers remain obscure. We have used a functional genomics approach to identify enzymes involved in carbohydrate biosynthesis and remodeling during xylem development in the hybrid aspen Populus tremula × tremuloides. Microarrays containing cDNA clones from different tissue-specific libraries were hybridized with probes obtained from narrow tissue sections prepared by cryosectioning of the developing xylem. Bioinformatic analyses using the sensitive tools developed for carbohydrate-active enzymes allowed the identification of 25 xylem-specific glycosyltransferases belonging to the Carbohydrate-Active EnZYme families GT2, GT8, GT14, GT31, GT43, GT47, and GT61 and nine glycosidases (or transglycosidases) belonging to the Carbohydrate-Active EnZYme families GH9, GH10, GH16, GH17, GH19, GH28, GH35, and GH51. While no genes encoding either polysaccharide lyases or carbohydrate esterases were found among the secondary wall-specific genes, one putative O-acetyltransferase was identified. These wood-specific enzyme genes constitute a valuable resource for future development of engineered fibers with improved performance in different applications.
- Published
- 2005
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