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The heat output of the non-growth metabolism of Chlorella vulgaris has been determined using an LKB-2277 BioActivity Monitor. The experimental results indicate that there is a turn-point on the metabolic thermogenic curves, which can be divided into two parts, an early phase and a later phase. For the early phase, there is a linear relationship between the metabolic power (P) and the cell concentration (C), and that the heat output produced by a single cell's metabolism (P0) depends on the cell concentration (C) and is inhibited by the cell density, the thermokinetic equation of their metabolism is dP/dt = k0, k0 = 0.