1. Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy
- Author
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Rosas-Rodríguez, Jesús A, Virgen-Ortíz, Adolfo, Ruiz, Enrico A, Ortiz, Rudy M, and Soñanez-Organis, José G
- Subjects
Biomedical and Clinical Sciences ,Genetics ,Digestive Diseases ,Biotechnology ,Heart Disease ,Cardiovascular ,Aetiology ,2.1 Biological and endogenous factors ,Reproductive health and childbirth ,Rats ,Female ,Animals ,Pregnancy ,Perilipin-1 ,Transcription Factors ,Peroxisome Proliferator-Activated Receptors ,Rats ,Sprague-Dawley ,Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ,Cardiomegaly ,Protein Isoforms ,RNA ,Messenger ,Triglycerides ,Cholesterol ,perilipin ,lipid metabolism ,pregnancy ,gene expression ,protein abundance ,PGC-1 alpha ,PGC-1α ,General & Internal Medicine ,Biomedical and clinical sciences ,Health sciences - Abstract
Background and Objectives: Perilipins 1-5 (PLIN) are lipid droplet-associated proteins that participate in regulating lipid storage and metabolism, and the PLIN5 isoform is known to form a nuclear complex with peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α) to regulate lipid metabolism gene expression. However, the changes in PLIN isoforms' expression in response to pregnancy-induced cardiac hypertrophy are not thoroughly studied. The aim of this study was to quantify the mRNA expression of PLIN isoforms and PGC-1α along with total triacylglycerol (TAG) and cholesterol levels during late pregnancy and the postpartum period in the rat left ventricle. Materials and Methods: Female Sprague-Dawley rats were divided into three groups: non-pregnant, late pregnancy, and postpartum. The mRNA and protein levels were evaluated using quantitative RT-PCR and Western blotting, respectively. TAG and total cholesterol content were evaluated using commercial colorimetric methods. Results: The expression of mRNAs for PLIN1, 2, and 5 increased during pregnancy and the postpartum period. PGC-1α mRNA and protein expression increased during pregnancy and the postpartum period. Moreover, TAG and total cholesterol increased during pregnancy and returned to basal levels after pregnancy. Conclusions: Our results demonstrate that pregnancy upregulates differentially the expression of PLIN isoforms along with PGC-1α, suggesting that together they might be involved in the regulation of the lipid metabolic shift induced by pregnancy.
- Published
- 2022