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Immunoassays are a powerful diagnostic tool and are widely used for the quantification of proteins and biomolecules in medical diagnosis and research. Enzyme-linked immunosorbent assay (ELISA) is the most commonly used immunoassay format and allows the detection of biomarkers at a very low concentration. The diagnostic platforms such as enzyme immunoassay (EIA), chemiluminescence (CL) assay, polymerase chain reaction (PCR), flow cytometry (FC), and mass spectrometry (MS) have been used to identify molecular biomarkers. However, these diagnostic tools requiring expensive equipment, long testing time, and qualified personnel that is not always available in small local hospitals with limited resources. The lateral flow immunoassay (LFIA) platform was developed for rapidly obtaining laboratory results and to make urgent decisions in emergency medicine, as well as the recently introduced concept of testing at the site of care (point-of-care, POC). The simultaneous measurement of different substances from a single sample called multiplex assays have become increasingly significant for in vitro quantification of multiple analytes in a single sample, thereby minimising cost, time, and volume. In multiplex immunoassays, the ligands are immobilized either in planar format (flat surface) or on microspheres in suspension that binds to target analytes in sample. The multiplex technology has established itself in proteomic networks and pathways, validation of genomic discoveries, and in the development of clinical biomarkers. In the present review article, various types of monoplex/simplex and complex/multiplex immunoassays have been analysed that are increasingly being applied in laboratory medicine. Also, some advantages and disadvantages of these multiplex assays have also been included such as experimental animals, in vitro tests using cell lines and tissue samples, 3-dimensional modelling and bioprinting, in silico tests, organ-on-chip, and computer modelling.

Objective To evaluate the detection rate (DR) by prenatal cell-free DNA test for pathogenic copy number variations (CNVs)＞2 Mb among pregnancies with fetal ultrasound abnormalities. Materials and methods This was a retrospective study on 29 pregnant women with fetuses diagnosed as microdeletion/microduplication syndromes by prenatal chromosome microarray analysis (CMA). Cell-free DNA from the maternal plasma was sequenced on the NextSeq CN500 sequencer. The quality standard of unique map reads in a single sample was greater than 10 M and only gains and losses of more than 2 Mb were reported. Results A total of 24 CNVs were identified by cell-free DNA test among the 21 fetuses with pathogenic CNVs identified by prenatal CMA, including 20 consistent CNVs and 4 inconsistent CNVs. Overall, the DR of cell-free DNA test for pathogenic CNVs ＞2 Mb was 69%. Microdeletions or microduplications at 22q11.2 were the most common CNVs, with a DR of 4/5 (80%) and 3/4 (75%) respectively. Conclusion Cell-free DNA test exhibited a moderate DR for pathogenic CNVs ＞2 Mb among fetuses with ultrasound abnormalities. Cell-free DNA test could provide an opportunity for early screening before the appearance of abnormalities on fetal ultrasound, while further clinical data and cost-effectiveness assessment are needed.

Relevance.The article highlights the problem of detecting residual amounts of antimicrobial substances in dairy products. The widespread use of medicines in veterinary medicine and animal husbandry creates certain problems associated with the possible contamination of raw materials and products with residual amounts of these substances in case of non-compliance with veterinary rules and regulations. On this basis it is necessary to monitor the content of drugs in animal products. Currently, microbiological and physico-chemical methods for the determination of antibacterial substances are used in Russia.Methods. O ne of the innovative directions in this field are methods, based on nanobiotechnology and, in particular, the immunomicrochip method. The immunomicrochip technology is designed for simultaneous qualitative and quantitative evaluation of several substances from a single sample.Results. During the research, the optimal parameters for detecting residual amounts of beta-lactam antibiotics, antibiotics of other groups and sulfonamides in milk and dairy products were determined by the method of immunomicrochip technology.

BackgroundGuidelines for venous blood sampling procedure (phlebotomy) discourage tourniquet use whenever possible. Here, we aimed to assess the Biomedical Scientists capability of not using the tourniquet in phlebotomy, which we hypothesized to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site.Materials and MethodsWe selected and assigned two (BMS) with the same age (41 years) and experience (20 years) to record ten phlebotomy days, the first with prioritized and the latter with non-prioritized patients. In a simple record form, each acquired daily data for the number of attended patients, age and gender, the frequency of non-tourniquet usage and the punctured vein. To test our work hypothesis we used the two-tailed single sample t-test (p < 0.05). Differences between age-group means and non-tourniquet use means by each BMS were tested by two-tailed t-test for independent means (p < 0.05).ResultsIn 10 phlebotomy days 683 patients were attended, with males representing 43,2% of the population. We found no statistically difference between age-group means. The combined capability of non-tourniquet use was 50,5%, which did not differ from our null hypothesis, but the individual group-means were statistically different, being 33% and 66.9% in the prioritized vs non-prioritized group. The medial cubital vein was the most prone to be punctured (77,7%).ConclusionsWe have shown that performing phlebotomies without tourniquet use is possible and desirable in at least half of the attended patients, though being more limited in specific group populations. Our results provide room for quality improvement in the laboratory pre-analytical phase.Key points summaryWe assessed the capability of Biomedical Scientists not using the tourniquet in real life blood sampling procedures for diagnostic purposes.Blood was collected from at least half of the attended patients without tourniquet use.Biomedical Scientists were able to prioritize the antecubital veins without tourniquet application (medial cubital vein the most prone to be punctured - 78% of attempts).

Background The 2018 BNMS Glomerular Filtration Rate (GFR) guidelines recommend a single-sample technique with the sampling time dictated by the expected renal function, but this is not known with any accuracy before the test. We aimed to assess whether the sampling regime suggested in the guidelines is optimal and determine the error in GFR result if the sample time is chosen incorrectly. We can then infer the degree of flexibility in the sampling regime. Methods Data from 6328 patients referred for GFR assessment at 6 different hospitals for a variety of indications were reviewed. The difference between the single-sample (Fleming) GFR result at each sample time and the slope–intercept GFR result at each hospital was calculated. A second dataset of 777 studies from one hospital with nine samples collected from 5 min to 8 h post-injection was analysed to provide a reference GFR to which the single-sample results were compared. Results Recommended single-sample times have been revised: for an expected GFR above 90 ml/min/1.73m2 a 2-h sample is recommended; between 50 and 90 ml/min/1.73m2 a 3-h sample is recommended; and between 30 and 50 ml/min/1.73m2 a 4-h sample is recommended. Root mean square error in single-sample GFR result compared with slope–intercept can be kept less than or equal to 3.30 ml/min/1.73m2 by following these recommendations. Conclusion The results of this multisite study demonstrate a reassuringly wide range of sample times for an acceptably accurate single-sample GFR result. Modified recommended single-sample times have been proposed in line with the results, and a lookup table has been produced of rms errors across the full range of GFR results for the three sample times which can be used for error reporting of a mistimed sample.

Motivation Accurate assembly of RNA-seq is a crucial step in many analytic tasks such as gene annotation or expression studies. Despite ongoing research, progress on traditional single sample assembly has brought no major breakthrough. Multi-sample RNA-Seq experiments provide more information than single sample datasets and thus constitute a promising area of research. Yet, this advantage is challenging to utilize due to the large amount of accumulating errors. Results We present an extension to Ryūtō enabling the reconstruction of consensus transcriptomes from multiple RNA-seq datasets, incorporating consensus calling at low level features. We report stable improvements already at three replicates. Ryūtō outperforms competing approaches, providing a better and user-adjustable sensitivity-precision trade-off. Ryūtō’s unique ability to utilize a (incomplete) reference for multi sample assemblies greatly increases precision. We demonstrate benefits for differential expression analysis. Ryūtō consistently improves assembly on replicates of the same tissue independent of filter settings, even when mixing conditions or time series. Consensus voting in Ryūtō is especially effective at high precision assembly, while Ryūtō’s conventional mode can reach higher recall. Availability and implementation Ryūtō is available at https://github.com/studla/RYUTO. Supplementary information Supplementary data are available at Bioinformatics online.

الملخص يستهدف البحث الحالي تحديد متطلبات استخدام الممارسة المبنية علي الأدلة في تحقيق الحماية الاجتماعية للأطفال المعرضين للخطر، ولتحقيق أهداف البحث تم تطبيق الدراسة علي عينة مکونة من عدد (67) مفردة من الأخصائيين الاجتماعيين العاملين بوحدات حماية الطفل، وتم استخدام أداه لقياس وتحديد متطلبات استخدام الممارسة المبنية علي الأدلة في تحقيق الحماية الاجتماعية للأطفال المعرضين للخطر، وقد کشفت نتائج الدراسة عن أهم المتطلبات اللازمة لتطبيق الممارسة المبنية علي الأدلة لتحقيق الحماية الاجتماعية للأطفال المعرضين للخطر جاءت مرتبة تنازليا کالتالي المتطلبات المعرفية والإدارية والمهنية والمهارية ، وفي النهاية تم التوصل إلي مؤشرات لوضع برنامج تدريبي للأخصائيين الاجتماعيين بوحدات حماية الطفل. الکلمات الدالة:( الممارسة المبنية علي الأدلة – الحماية الاجتماعية – الأطفال المعرضين للخطر). Abstract The current research aims to determine the requirements for using evidence-based practice in achieving social protection for children at risk, and to achieve the objectives of the research, the study was applied to a single sample of (67) social workers working in child protection units ,A tool was used to measure and define the requirements for using evidence-based practice in achieving social protection for children at risk, and the results of the study revealed the most important requirements for applying evidence-based practice to achieve social protection for children at risk, ranked in descending order, as follows: cognitive, administrative, professional and skill requirements, and in the end Indicators were reached to develop a training program for social workers in the Child Protection Units. Key words: (Evidence-Based Practice – Social protection – Children At Risk).

To solve the small sample problem of biometric identification, this paper investigates the limiting case of the problem, i.e., the recognition of a single training sample, and proposes a single sample discriminant analysis method based on Gabor wavelet and KPCA-RBF (KPRC) classifier (kernel principal component analysis-radial basis function). The proposed method performs pixel-level fusion of face and palmprint images. Firstly, a face image and a palmprint image were subject to two-dimensional (2D) Gabor wavelet transform. The resulting Gabor face image and Gabor palmprint image were fused on the pixel level into a new fused image. Next, a new classifier called KPCA-RBF was designed to extract nonlinear discriminative features by KPCA, and classify objects with RBF. Based on AR database, FERET database, and palmprint database, the single sample discriminant analysis method was realized based on Gabor transform and KPCA-RBF classifier. Experimental results show that multimodal recognition methods clearly outshine single-modal recognition methods, and the GABOR-KPRC with pixel-level fusion achieves better recognition effect than other fusion methods. It was also demonstrated that Gabor transform and KPRC classifier can effectively improve the fusion effect, whether for pixel-level fusion or decision-level fusion.

Benign Paroxysmal Positional Vertigo (BPPV) adalah gangguan vestibular yang paling sering menyebabkan vertigo. BPPV dapat diartikan sebagai vertigo yang terjadi akibat perubahan posisi kepala secara tiba-tiba. Semont Liberatory Maneuver dapat mengurangi vertigo pada BBPV. Semont Liberatory Maneuver bertujuan untuk memindahkan otolit dari dalam area sensitif telinga sehingga dapat mengurangi gejala vertigo. Tujuan dari penelitian ini adalah untuk mengetahui penatalaksanaan fisioterapi menggunakan Semont Liberatory Maneuver dalam mengurangi vertigo terhadap penderita BPPV. Penelitian ini menggunakan metode case study dengan sampel tunggal dengan alat ukur vertigo symptom scale-short form (VSS-sf). Dari hasil penelitian didapatkan hasil semont liberatory maneuver yang dilakukan sebanyak 8 kali selama 8 hari berturut-turut dengan intensitas 4x pengulangan selama 15 menit dapat menurunkan vertigo pada kondisi BPPV. Saran kepada peneliti selanjutnya untuk mencari tahu efek lain dari semont liberatory maneuver selain untuk menurunkan frekuensi vertigo pada BPPV.

This study investigated developmental trajectories of reading and math using latent-growth-curve analyses across multiple academic skills, measures, and multiple time periods within a single sample. Reading-related growth was marked by significant individual differences during the early elementary-school period and nonsignificant individual differences during the late elementary-school period. For math-related skills, nonsignificant individual differences were present for early math growth and significant individual differences were present in late elementary-school. No clear pattern of cumulative, compensatory, or stable development emerged for either reading-related or math skills. These differing growth patterns highlight developmental complexities and suggest domain-specific differences in achievement growth that are potentially associated with contextual factors. (PsycInfo Database Record (c) 2021 APA, all rights reserved)

The 40Ar/39Ar dating method is among the most versatile of geochronometers, having the potential to date a broad variety of K-bearing materials spanning from the time of Earth’s formation into the historical realm. Measurements using modern noble-gas mass spectrometers are now producing 40Ar/39Ar dates with analytical uncertainties of ~0.1%, thereby providing precise time constraints for a wide range of geologic and extraterrestrial processes. Analyses of increasingly smaller subsamples have revealed age dispersion in many materials, including some minerals used as neutron fluence monitors. Accordingly, interpretive strategies are evolving to address observed dispersion in dates from a single sample. Moreover, inferring a geologically meaningful “age” from a measured “date” or set of dates is dependent on the geological problem being addressed and the salient assumptions associated with each set of data. We highlight requirements for collateral information that will better constrain the interpretation of 40Ar/39Ar data sets, including those associated with single-crystal fusion analyses, incremental heating experiments, and in situ analyses of microsampled domains. To ensure the utility and viability of published results, we emphasize previous recommendations for reporting 40Ar/39Ar data and the related essential metadata, with the amendment that data conform to evolving standards of being findable, accessible, interoperable, and reusable (FAIR) by both humans and computers. Our examples provide guidance for the presentation and interpretation of 40Ar/39Ar dates to maximize their interdisciplinary usage, reproducibility, and longevity.

Gopalan et al. (2021) present multi-CUT&Tag, a modification of cleavage under targets and tagmentation (CUT&Tag) that enables simultaneous genome-wide mapping of multiple chromatin-associated targets in a single sample.

This research was conducted to examine primary school students' attitudes towards music education lessons according to certain variables. 64 elementary schools from Kastamonu in Turkey participated in the study. The attitude scale developed by Gullu and Guclu was adapted to the Music Education Courses Attitude scale and was used to determine students' attitudes towards music education lessons. In the statistical analysis of the data obtained as a result of the study, frequency, Single Sample Kolmogorov-Smirnov, Mann-Whitney U and Kruskal-Wallis H analysis were performed in SPSS 15.0 for Windows program. From the research result, it is concluded that most of the students (63.9%) have the habit of listening to music regularly and the number of students who participate in music activities and make music is quite high (43.8%). When the attitudes of students towards music education lessons were examined, it was seen that students' attitudes towards music education lessons were generally high. In addition, it was concluded that students' attitudes towards music education lessons did not show a statistically significant difference in terms of gender and age groups (P> 0.05). It was determined that the participation of students in music activities positively affected their attitudes towards music education lessons and this result was found to be statistically significant. This study is important in terms of guiding field experts, researchers and interests. Key words: Music, education, music education, student behavior, primary education.

Antimicrobial resistance is increasingly recognized as a major threat to global health. To combat this emerging threat, accessible antimicrobial susceptibility testing should be prioritized as a key component of stewardship efforts. In this work, we developed a user-friendly paper-based test that provides visual readout of bacterial antibiotic susceptibility in a semiquantitative format. We leveraged on-chip paper microfluidics to enable multiplexed testing of multiple antibiotic dilutions with a single sample addition step, replicating the functionality of traditional broth-dilution-based susceptibility testing in a simplified format. Our paper-based test offers several advantages including low sample volume requirement and lack of need for humidity control during incubation, an innovation that addresses a key limitation of conventional paper-microfluidic devices. Using several clinically relevant bacterial organisms and antimicrobial agents, we demonstrate that our colorimetric readout approach provides a strong predictor of susceptibility category.

The study demonstrates a new method to quantify Secondary Ion Mass Spectrometry (SIMS) data by using a synergetic combination of Energy Dispersive X-ray spectroscopy (EDX) and SIMS. The novelty of this approach lies in using a diffusion couple to produce a continuum of concentration variation to cover a large portion of the composition space (100 at% to sub-1000 ppm) in a single sample. Furthermore, this approach encompasses the concentration-dependent Relative Sensitivity Factor (RSF) which is essential when the concentrations span several orders of magnitude. By correlating EDX and SIMS profiles, the SIMS signal intensity can be converted to concentrations far below the detection limit of the EDX technique.

Koura-koura is a product resulting from the processing of peanut. This study consisted of producing koura-koura with garlic, pepper, ginger and mint to reduce aflatoxins and microorganisms. The objective of this work is the decontamination of koura-koura with spices and aromatic leaves. Aflatoxin determination was performed by HPLC and microbiological analyses were carried out according to standard methods. A total of 18 samples were analyzed, including 3 peanut samples, 2 peanut paste samples, 1 koura-koura control sample, and 12 samples of koura-koura spices and aromatic leaves. Total aflatoxin B1, B2, and aflatoxin levels in the samples ranged from 0.58±0.49 ?g/kg to 3.66±0.10 ?g/kg; 2.23±0.41 ?g/kg to 14.02±0.88 ?g/kg; 2.87±0.20 ?g/kg to 17.75±0.58 ?g/kg, respectively. Aflatoxins G1 and G2 were not detected in all samples. The total mesophilic aerobic flora (TMAF) ranged from 1.60±1.57 x101 CFU/g to 4.50±1.28 x105 CFU/g and the yeast and mould flora ranged from 1.80 ± 1.68 x101 CFU/g to 2.80±0.74 x101 CFU/g. No samples were contaminated with thermo-tolerant coliforms and total coliforms were present in a single sample (1.30 ± 1.64 x 101 CFU/g). The results of the study on the reduction of aflatoxins and microorganisms in koura-koura with spices and aromatic leaves contribute significantly to food safet

Objective The purpose of this study was to better understand conflicting findings in the literature regarding the adjustment of siblings of children with cancer by examining, in a single sample, differences in patterns of results as a function of reporter and comparator used (i.e., population norms, demographically matched classmates). Method Self- and parent-report standardized measures of depression, anxiety, and behavioral problems were collected for 67 siblings and 67 demographically matched classmates. Comparisons were made to norms and controls. Results Siblings consistently demonstrated poorer psychosocial functioning than their demographically matched peers across all measures but their scores did not differ from norms. A significantly greater percentage of siblings fell outside the normal range than that expected in the general population for parent-reported total and internalizing problems, but not for externalizing problems or the self-report measures. Conclusions Findings regarding the psychological adjustment of siblings of children with cancer differ according to the research methods used. It is important to use rigorous methods such as demographically matched peer comparisons when investigating the impact of childhood cancer on siblings.

Ribonucleic acid (RNA)-seq data contain not only host transcriptomes but also nonhost information that comprises transcripts from active microbiota in the host cells. Therefore, joint and integrative analyses of both host and meta-transcriptome can reveal gene expression of the microbial community in a given sample as well as the correlative and interactive dynamics of the host response to the microbiome. However, there are no convenient tools that can systemically analyze host–microbiota interactions through simultaneously quantifying the host and meta-transcriptome in the same sample at the tissue and the single-cell level. This poses a challenge for interested researchers with limited expertise in bioinformatics. Here, we developed a software pipeline that can comprehensively and synergistically analyze and correlate the host and meta-transcriptome in a single sample using bulk and single-cell RNA-seq data. This pipeline, named meta-transcriptome detector (MTD), can extensively identify and quantify microbiome, including viruses, bacteria, protozoa, fungi, plasmids and vectors, in the host cells and correlate the microbiome with the host transcriptome. MTD is easy to install and run, involving only a few lines of simple commands. It offers researchers with unique genomics insights into host responses to microorganisms.

The aim of the present study was to quantify a large number of analytes including opioids, stimulants, benzodiazepines, z-drugs, antidepressants and neuroleptics within a single sample workup followed by a single analytical measurement. Expected drug concentrations in hair are strongly substance dependent. Therefore, three different calibration ranges were implemented: 0.5 to 600 pg/mg (group 1), 10 to 12,000 pg/mg (group 2) and 50 to 60,000 pg/mg (group 3). In order to avoid saturation effects, different strategies were applied for selected transitions including the use of parent mass ions containing one or two 13C-isotopes and detuning of the declustering potential and/or collision energy. Drugs were extracted from pulverized hair by a two-step extraction protocol and measured by liquid chromatrography--tandem mass spectrometry (LC--MS-MS) using Scheduled MRM™ Algorithm Pro. In total, 275 MRM transitions including 43 deuterated standards were measured. The method has been fully validated according to international guidelines. A MultiQuant™ software based tool for task-oriented data evaluation was established, which allows extracting selected information from the measured data sets. The matrix effects and recoveries were within the allowed ranges for the majority of the analytes. The lower limits of quantification (LLOQs) were for ∼72% of the analytes in the low-pg/mg range (0.5–5 pg/mg) and for ∼24% of the analytes between 10 and 50 pg/mg. These LLOQs considered cut-offs by the Society of Hair Testing (SoHT), if recommended. The herein established multi-analyte approach meets the specific requirements of forensic hair testing and can be used for the rapid and robust measurement of a wide range of psychoactive substances. The analyte-specific wide concentration ranges open up a wide field of applications.

This research aims to know the effect of Interval Training on VO2 Max in the extracurricularPencak Silat Brotherhood Faithful Heart Terate Rayon Special smkn 1 Nanga Pinoh.This research includes pre-experiment, with the sample not separate. The experimental methodwith the sample does not mean that the research has a single sample group, measured twice, thefirst measurement is done before the subject ispretests,then treatment,which is finally closed witha second measurement (posttest). The design used in this study was "The One Group PretestPosttest Design" or the absence of a control group.The results of the previous study anddiscussion obtained obtained the calculated T value (2.630) t Table (1.812), and the value ofsig (2-tailed) (0.000) of 0.05, the result indicates that the value of T count is greater than in Ttable. The result is interpreted Ha: accepted and Ho: rejected. If Ha accepted interpreted theeffect of Interval Training on VO2 Max on the extra-curricular Pencak Silat Brotherhood ofFaithful Heart Terate Rayon Special SMKN 1 Nanga Pinoh.Keywords: Interval Training, VO2 Max, Pencak Silat.Abstrak: Penelitian ini bertujuan ingin mengetahui Pengaruh Interval Training Terhadap VO2Max Pada Ekstra Kurikuler Pencak Silat Persaudaraan Setia Hati Terate Rayon Khusus SMKN1 Nanga Pinoh. Penelitian ini termasuk pre-experiment, dengan sampel tidak terpisah. Metodeeksperimen dengan sampel tidak terpisah maksudnya penelitianya memiliki satu kelompoksampel saja,yang diukur dua kali, pengukuran pertama dilakukan sebelum subjek diberiperlakuan (pretest), kemudian perlakuan (treatment),yang akhirnya ditutup dengan pengukurankedua (posttest). Desain yang digunakan dalam penelitian ini adalah“The One Group PretestPosttest Design” atau tidak adanya grup kontrol. Hasil penelitian dan pembahasan sebelumnyadiperoleh nilai t hitung (2.630)t Tabel (1.812), dan nilai sig (2-tailed) (0,000) dari 0,05, hasiltersebut menunjukkan bahwa nilai t hitung lebih besar dari pada t tabel. Hasil tersebut diartikanHa: diterima dan Ho: ditolak. Jika Ha diterima diartikan ada Pengaruh Interval TrainingTerhadap VO2 Max Pada Ekstra Kurikuler Pencak Silat Persaudaraan Setia Hati Terate RayonKhusus SMKN 1 Nanga Pinoh.Kata kunci : Interval Training, VO2 Max, Pencak Silat.

الملخص: في ظل التطور الهائل في تکنولوجيا المعلومات والاتصالات وسعي الحکومات والمنظمات کافة لاستخدامها في تسيير اعمالها وذلک لتقديم خدمات الرعاية الاجتماعية ذات جودة افضل والاستجابة بأسرع وقت وتحقيق الاندماج ما بين المصلحة (مواطنين –عاملين - منظمات) من خلال المشارکة في اتخاذ القرار وتوفير بيئة مواتية لتطور اقتصادي سليم وکل ذلک يتحقق من خلال تطبيق الحوکمه الالکترونية بالاعتماد على تکنولوجيا المعلومات والاتصالات. وتعد الحکومة الالکترونية واحده من اهم المبادرات على مستوى العالم لتقديم الخدمات غير الحکومية عبر وسائل الاتصال المختلفة للمواطنين جانب ودعم وتفعيل استخدام تکنولوجيا المعلومات بشکل مکثف حيث يتضمن ذلک تحويل المحتوى المعلوماتي بالمؤسسات والمنظمات الى محتوى رقمي ودعم البنية الاساسية لتکنولوجيا المعلومات للاعتماد عليها في تقديم خدمات الرعاية الاجتماعية بالمنظمة، ولذا فقد استهدفت هذه الدراسة- الوصفية- التعرف على مجموعة من المتطلبات اللازمة لتطوير خدمات الرعاية الاجتماعية من خلال اختبار مجموعة من الفروض ، وقد طبقت على عينة (84) مفردة من العاملين بتلک المنظمات، وقد خرجت الدراسة بمجموعة من التوصيات قد تفيد العاملين بتلک المنظمات في استخدام وتطبيق نظم الحوکمه الالکترونية في تقديمها لخدمات الرعاية الاجتماعية بالمنظمة بشکل أفضل مما کانت علية. In light of the tremendous development in information and communication technology and the endeavor of all governments and organizations to use them in the conduct of their business in order to provide better quality social welfare services and respond in a faster time and achieve integration between the interest (citizens - workers - organizations) through participation in decision-making and providing an environment conducive to economic development All this is achieved through the application of electronic governance, relying on information and communication technology. The e-government is one of the most important initiatives in the world to provide non-governmental services through various means of communication to citizens, in addition to supporting and activating the intensive use of information technology, as this includes converting information content in institutions and organizations into digital content and supporting the infrastructure of information technology to rely on in the provision of social care services Therefore, this descriptive study aimed at identifying a set of requirements for developing social care services by testing a set of hypotheses, and it was applied to a single sample (84) of workers in those organizations, The study came out with a set of recommendations that may benefit workers in these organizations in using and applying electronic governance systems in providing social care services in the organization better than it was before.

الملخص العربى : هدف البحث إلى وضع رؤية مقترحة لدور معلمي مدارس التعليم العام بمدينة أبها الحضرية في تحقيق أبعاد المواطنة الرقمية (التمکين الرقمي، والتواصل الرقمي، والأمن الرقمي، والصحة والسلامة الرقمية) لطلابها، واستخدم البحث المنهج الوصفي والاستبانة کأداة للبحث طبقت على عينة بلغت (375) مفردة من القادة والمشرفين والمشرفات والمعلمين والمعلمات بتلک المدارس؛ وأسفر البحث عن وضع الرؤية المقترحة، وموافقة أفراد عينة البحث حول أهمية دور المعلم في تحقيق أبعاد المواطنة الرقمية (التمکين الرقمي، والتواصل الرقمي، والأمن الرقمي، والصحة والسلامة الرقمية) لطلابها بدرجة" کبيرة"، وعدم وجود فروق ذات دلالة إحصائية في استجابات أفراد عينة البحث حول دور المعلم في تحقيق أبعاد (التمکين الرقمي، والتواصل الرقمي، والأمن الرقمي) للمواطنة الرقمية للطلبة تعزى لمتغير الوظيفة الحالية، والمرحلة التعليمية، ووجود فروق ذات دلالة إحصائية بين استجابات أفراد عينة البحث حول المحور الرابع المتعلق بدور المعلم في تحقيق بعد (الصحة والسلامة الرقمية) للمواطنة الرقمية للطلبة تعزى لمتغير الوظيفة الحالية بين فئة القائد والمشرف، وفئة المعلم والمشرف؛ وأوصى البحث بعقد دورات تدريبية لقادة المدارس والمعلمين حول مهارات محو الأمية الرقمية، وتدريب المعلمين على توظيف التقنيات الرقمية في العملية التعليمية، وإدخال المواطنة الرقمية ومجالاتها المختلفة في المقررات بمراحل التعليم المختلفة للتوعية بالاستخدام الأمن للتکنولوجيا الرقمية. الملخص الانجليزى : The aim of the research was to develop a proposed vision of the role of public education school teachers in the urban city of Abha in achieving the dimensions of digital citizenship (digital empowerment, digital communication, digital security, health and digital safety) for their students, and the research used descriptive and questionnaire method as a research tool applied to a single sample of 375 From leaders, supervisors, supervisors, teachers and teachers in those schools, the research resulted in the development of the proposed vision, and the approval of the research sample members on the importance of the role of the teacher in achieving the dimensions of digital citizenship (digital empowerment, digital communication, digital security, digital health and safety) to its students to a degree." The absence of statistically significant differences in the responses of the research sample members about the role of the teacher in achieving dimensions (digital empowerment, digital communication, digital security) of student digital citizenship due to the change in current job, educational stage, and statistically significant differences between the responses of the research sample members on the fourth axis of the teacher's role in achieving the digital citizenship of students is due to the change in the current function between the leader, supervisor, teacher and supervisor; Digital literacy, training teachers to employ digital technologies in the educational process, and introducing digital citizenship and its various fields in courses at different levels of education to raise awareness of the security use of digital technology.

The demand for monofloral, original, and special (functional) kinds of honey, or those with geographical indication, is forecast. At the same time, there is a need to improve the methods for determining the botanical and geographical origin of honey. The purpose of the research was to select and apply a variety of techniques for identifying the botanical origin of honey for its correspondence to acacia species. Samples of honey from the Kyiv, Odesa, and Dnipro regions extracted in the spring and summer period were used in the research. Organoleptic, physicochemical, NMR spectrometry, and advanced melissopalynology methods were applied. The tests were carried out at the laboratories of the Department of Certification and Standardization of Agricultural Products, NULES, Ukraine; the Ukrainian Laboratory of Quality and Safety of Agricultural Products; and the Bruker BioSpin GmbH company (Germany). According to the research results, the requirements for acacia honey were met by the organoleptic method for samples B1 and B2; by the physicochemical method for A0 and A2; by NMR spectroscopy for not a single sample, all being assessed as polyfloral; and by pollen analysis for B1 and B2. The conducted studies confirm the need for a comprehensive approach to the identification of the botanical origin of honey for its conformity to acacia species. There is a need to review the physicochemical indicators for the compliance of honey with the acacia species obtained in Ukraine. After all, even the modern NMR spectrometry technique indicated that the specially fabricated sample that did not contain acacia pollen grains was acacia honey. Identification of the botanical origin of monofloral honey, in particular acacia, should be carried out in the following sequence: pollen analysis (by dominant pollen grains), safety (presence of antibiotics, pesticides), physicochemical parameters according to international requirements, organoleptic parameters.

Sakarya, Şakir (Balikesir Author), Bu çalışmada, 2007-2017 dönemi arasında Borsa İstanbul’da işlem gören bankalarda meydana gelen operasyonel kayıp olaylarının itibar riskine neden olup olmadığı incelenmiştir. Bu araştırmada olay çalışması yöntemi ve tek örneklem t testi analizi kullanılmıştır. Olay çalışması yöntemi ile 3 günlük ve 21 günlük olay penceresinde operasyonel kayıp duyurularının itibar riskine etkisi araştırılmış ve sonuç olarak sadece kayıp duyurularının yapıldığı gün (olay günü) negatif anormal getiriler tespit edilmiştir. Olay gününden uzaklaşıldıkça duyuruların etkisinin kaybolduğu gözlemlenmiştir., In this study, it was investigated whether the operational loss incidents that occurred in banks traded in Borsa Istanbul between 2007 and 2017 cause reputational risk. In this research, case study method and single sample t test analysis were used. The effect of operational loss announcements on reputation risk was investigated via event study method in terms of events for 3 and 21 days and as a result, negative abnormal yields were determined only on the day the announcement loss were made (event day). It was observed that the announcements lost their effects as long as it was moved away from the event day.

Multi-label learning (MLL) solves the problem that one single sample corresponds to multiple labels. It is a challenging task due to the long-tail label distribution and the sophisticated label relations. Semi-supervised MLL methods utilize a small-scale labeled samples and large-scale unlabeled samples to enhance the performance. However, these approaches mainly focus on exploring the data distribution in feature space while ignoring mining the label relation inside of each instance. To this end, we proposed a Dual Relation Semi-supervised Multi-label Learning (DRML) approach which jointly explores the feature distribution and the label relation simultaneously. A dual-classifier domain adaptation strategy is proposed to align features while generating pseudo labels to improve learning performance. A relation network is proposed to explore the relation knowledge. As a result, DRML effectively explores the feature-label and label-label relations in both labeled and unlabeled samples. It is an end-to-end model without any extra knowledge. Extensive experiments illustrate the effectiveness and efficiency of our method1.

Purpose: To compare two transformational leadership instruments, Bass’s Full Range Leadership Model and its instrument Multifactor Leadership Questionnaire with the Transformational Leadership Scale proposed by Rafferty and Griffin (2004), using empirical evidence from a single sample.

espanolObtener extracciones de ADN de buena calidad es un paso crucial para los proyectos de investigacion del ambito de la biologia molecular. En la actualidad, existen numerosos protocolos para la extraccion en tejidos de vertebrados. En el presente estudio comparamos 11 protocolos de extraccion por columnas y un protocolo HotSHOT utilizando condiciones parecidas (como el tipo de muestra o el peso del material inicial). Evaluamos el tiempo de extraccion, la calidad y la cantidad de ADN obtenido y el precio de la extraccion de una unica muestra. Segun nuestro analisis, los kits que dieron mejores resultados para producir ADN con la mayor concentracion y pureza son JetQuick® Genomic DNA Purification Kit (Genomed) y el NucleoSpin® Tissue (Macherey–Nagel). No obstante, se recomienda encarecidamente probar varios kits de extraccion con muestras especificas para encontrar el que sea mejor en cuanto al tiempo, la calidad y el costo en relacion con un proyecto concreto. EnglishObtaining high quality DNA extractions is a crucial step for molecular biology research projects. At present, numerous protocols are available for vertebrate tissue extractions. In the present study we compared eleven column–based protocols and one HotSHOT protocol using similar conditions (i.e., type of sample, weight of starting material). We evaluated time of extraction, quality and quantity of DNA yield, and price of extraction for a single sample. Based on our analysis, the most successful kits for producing DNA with the highest concentration and purity are the JetQuick® Genomic DNA Purification Kit (Genomed) and the NucleoSpin® Tissue (Macherey–Nagel). Nevertheless, it is highly recom-mended to test various extraction kits with specific samples to find the optimal kit in all aspects of time, quality and cost for a particular project

We study single-sample prophet inequalities (SSPIs), i.e., prophet inequalities where only a single sample from each prior distribution is available. Besides a direct, and optimal, SSPI for the basic single choice problem [Rubinstein et al., 2020], most existing SSPI results were obtained via an elegant, but inherently lossy, reduction to order-oblivious secretary (OOS) policies [Azar et al., 2014]. Motivated by this discrepancy, we develop an intuitive and versatile greedy-based technique that yields SSPIs directly rather than through the reduction to OOSs. Our results can be seen as generalizing and unifying a number of existing results in the area of prophet and secretary problems. Our algorithms significantly improve on the competitive guarantees for a number of interesting scenarios (including general matching with edge arrivals, bipartite matching with vertex arrivals, and certain matroids), and capture new settings (such as budget additive combinatorial auctions). Complementing our algorithmic results, we also consider mechanism design variants. Finally, we analyze the power and limitations of different SSPI approaches by providing a partial converse to the reduction from SSPI to OOS given by Azar et al., Accepted to SODA'22. Merges and extends arXiv:2103.13089 [cs.GT] and arXiv:2104.02050 [cs.DS]