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OBJECTIVE: Fibromyalgia (FM) is a clinical syndrome characterized by prominent physical and psychological impairment and widespread pain on both sides of the body, above and below the waist, and along the axial skeleton. It often causes sleep difficulties, memory impairment, mood changes, irritable bowel syndrome, and fatigue. Our study aimed to investigate the relationship between FM and prolidase (peptidase D) and histone H3 protein levels by comparing a patient group with a healthy control group. PATIENTS AND METHODS: In total, 176 people were examined in our study, 88 of whom were healthy and 88 of whom had FM. Serum level was measured by ELISA. Then the results were analyzed using SPSS. All p < 0.05 were considered statistically significant. RESULTS: A significant increase in the levels of prolidase was observed in the patient group compared with the control group (6.28-4.68, p

Background: Breast cancer is a leading cause of death in women worldwide. Genetic polymorphisms have been reported to be important etiological factors. Murine double minute 2 (MDM2) T309G interacts with p53 and mutations in p53 are present in approximately 50% of all cancers. However, it has been reported that effect of the polymorphism on breast cancer risk may vary in different populations. Here, we therefore investigated whether there is an association between MDM2 T309G (rs2279744) polymorphism and breast cancer in a Turkish population. Materials and Methods: We analysed 110 patients with breast cancer and 138 matched? controls. For genotyping, polymerase chain reaction and restriction length fragment polymorphism methods were used. Results: A significant difference was observed between case and control groups with regard to the distribution of the MDM2 T309G polymorphism (p

Amaç: Akciğer kanseri dünya genelinde kansere bağlıölümlerin önde gelen nedenidir. Tanısal cihazlar ve teknolojik gelişmelerebağlı tedavi seçeneklerinde ilerlemeye rağmen, akciğer kanseri hastalarındagenel mortalite oranı hala yüksektir. Akciğer kanseri hastalarında sağkalımoranları, özellikle ilerlemiş inoperabl hastalıkta, immunoterapi gibi yenitedavi seçeneklerine rağmen düşüktür. p53, kanser hastalarının %60-70’inde mutasyonauğramaktadır ve bu nedenle son zamanlarda yapılan çalışmalar göstermektedir ki,serum anti-p53 antikorunun, over, özefagus, meme ve akciğer kanseri gibi bazıkanser türlerinin dedekte edilmesinde biyobelirteç olarak dikkate alınabilir.Bu çalışmada, akciğer kanseri hastalarında, serum anti-p53antikor düzeylerinintanısal ve prognostik önemini araştırmayı amaçladık.Yöntem: Çalışmayaakciğer kanseri (AK) tanısı nedeniyle evreleme için 18F-FDG-PET / BTgörüntüleme amacı ile bölümümüze sevk edilen hastalar, toraks BT’sinde şüphelipulmoner nodül olup, patolojik FDG birikimi göstermeyen hastalar (NAPN= Non-avid FDG gösterenpulmoner nodül) ve sağlıklı gönüllüler dahil edildi. Serum anti-p53antikordüzeyleri tüm hastalarda ELISA yöntemi ile ölçüldü. Hastaların ortalama takipsüresi 13 ay idi.Bulgular: Çalışmaya toplam 65 AK hastası (58E/7K), 47 NAPNhastası (20E/27K) ve 34 sağlıklı gönüllü (26E /8K) dahil edildi. Ortalama serumanti-p53antikor seviyeleri AK hastalarında 3.4ng/mL, NAPN hastalarında 3.77ng/mL, sağlıklı gönüllülerde 3.07 ng/ mL idi. AK hastaları ile NAPN hastaları arasındaserum anti-p53antikor düzeyi için istatistiksel olarak anlamlı fark yoktu (p =0.678). Hatta, hastalarve sağlıklı gönüllüler arasında serum anti-p53Ab düzeyi için istatistikselolarak anlamlı fark yoktu (p = 0.377). Hastaların iki yıllık medyan sağkalımı14 aydı. Hastalarınsağkalım hızında, serum anti-p53Ab düzeyinin > 3.4 ng/ mL veya ≤3.4 ng/ mL olmasının herhangi bir etkisininolmadığı bulundu (p = 0.652).Sonuç: Anti-p53antikoru,karsinogeneziste çok önemli olmasına rağmen, serum anti-p53antikor düzeyininakciğer kanseri tanısında ve sağkalım oranlarında tek başına önemli olmadığını düşünüyoruz. Karsinogenezistebirden fazla faktör vardır ve bu durumun nedeni olabilir. Akciğer kanserihastalarının teşhisi için serum anti-p53antikor düzeylerinin bilinen bir katofdeğeri yoktur. Bu nedenle, buantikorun tümör spesifikliği olmadığını ve serum anti-p53antikor düzeyininakciğer kanseri taraması için uygun olmadığını düşünüyoruz., Objective: Lung cancer is the leading causeof cancer-related deaths worldwide. Despite advancement in diagnostic tools andtreatment options with technological developments, overall mortality rates inlung cancer patients remains high. Survival rates in lung cancer patients islow especially in advanced diseased inoperable patients in spite of newtreatment options like immunotherapy. p53 is mutated in 60-70% of cancerpatients and for this reason has been extensively studied recent researches suggest that serumanti-p53Ab can be considered as biomarkers to detect many types of cancers; asovarian cancer, esophageal cancer, breast cancer and lung cancer. In this studywe aimed that are there any diagnostic and prognostic importance of serumanti-p53Ab levels in lung cancer patients.Method: Patients were included who werereferred to our department with the purpose of 18F-FDG-PET/CTimaging for staging due to lung cancer diagnosis (LC) and patients who wereperformed 18F-FDG-PET/CT for diagnosis in the cause of the suspectedpulmonary nodule in thorax CT but not detected pathologic FDG accumulation(NAPN=pulmonary nodule with non-avid-FDG) and healthy volunteers. Serumanti-p53Ab levels were measured with ELISA method in the all patients. Meanfollow up time of patients were 13 months.Results: A total of 65 LC patients(58M/7F), 47 patients with NAPN (20M/27F), and a total of 34 healthy volunteers(26M/8F) were included in this study. Median serum anti-p53Ab levels are3.4ng/mL in LC patients, 3.77ng/mL in NAPN patients, 3.07ng/mL in healthyvolunteers. There is no statistically significant difference for serumanti-p53Ab level between LC patients and NAPN patients (p=0.678). Moreover there is no statistically significant differencefor serum anti-p53Ab level between patients and healthy volunteers (p=0.377). Two-year median survival ofpatients was 14 month. It has been found that there is no effect of serumanti-p53Ab level whether >3.4 or ≤3.4 on the patient survival rate (p=0.652).Conclusions: Even thoughanti-p53Ab is very important in carcinogenesis, we think that serum anti-p53Ablevel by itself is not important in lung cancer diagnosis and survival rates.There are multiple factors in carcinogenesis and this may be the reason of thissituation. There is no known cut off value of serum anti-p53 Ab levels fordiagnosis of lung cancer patients. Therefore we think that this antibody is nottumor spesific and serum anti-p53 Ab level measurement is not appropriate forlung cancer screening.

WOS: 000454950700019, Objective: The study was aimed to evaluate the cytotoxic activity of acetone extract of leaves, flower and body of Euphorbia macroclada boiss on human breast cancer cell line (MCF-7). Material: The cells were plated at a cell density of 1x10(5) cells in 96-well plates and grown with DMEM medium containing supplemented with 10% FBS and 1% penicillin. The cells were treated by different concentrations of acetone extract of Euphorbia macroclada boiss (10-1000 mu g/mL) during 24, 48 and 72 h. The cytotoxic activities of the tested compounds were determined by cell proliferation analysis using standard (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: After the evaluation of cytotoxicity assay results, it is determined that flower and body parts have a significant cytotoxic effect on MCF-7 breast cancer cell line. The values that obtained reading at 570 nm spectrophotometrically, were analyzed with GraphPad Prism7 and IC50 growth inhibition values was determined. Conclusion: The results of MTT assay showed that leaves, flower and body significantly reduced % cell viability comparative to the control. It was also shown that body had more growth inhibitory effect on MCF-7 cell compared to the leaves part.

WOS: 000461242900006, PubMed ID: 30906548, The present study aimed to investigate the possible association between the genetic polymorphism of the enzyme superoxide dismutase 2 (SOD2, also known as manganese-dependent SOD), Ala16Val (rs4880), and primary brain tumor risk in the Turkish population. Frequency of the SOD2 gene rs4880 polymorphism was identified in 225 Turkish individuals (120 controls and 105 patients with primary brain tumor) by polymerase chain reaction-restriction fragment length polymorphism. Subject demographics and clinical characteristics were also recorded. The findings were evaluated using logistic regression and chi(2) tests. Logistic regression analysis indicated that smoking did not increase the risk for primary brain tumor [ odds ratio (OR)= 0.77, 95% confidence interval (CI)= 0.44-1.33, chi(2)= 0.352, P= 0.860]. Similarly, there was no statistically significant difference in the family history of cancer incidence between the control subjects and the primary brain tumor patients (OR= 0.81, 95% CI= 0.39-1.71, chi(2)= 0.340, P= 0.560). There was no significant association of the histopathological type, genotype/ allele frequencies and inheritance models of tumor with the gene variants among the patients with primary brain tumor. In summary, the results of the present study indicated that the Ala16Val polymorphism of the SOD2 gene was not associated with primary brain tumor risk in the Turkish population studied., Sivas Cumhuriyet University (Sivas, Turkey) [CUBAP T-403], The current study was funded by Sivas Cumhuriyet University (Sivas, Turkey) via research grants (grant no. CUBAP T-403) provided to the project.

WOS: 000405114700002, Introduction: Association between Murine double minute 2 T309G polymorphism and lung cancer risk has been investigated in several populations, but results of these studies are inconsistent. We aimed to investigate the effect of Murine double minute 2 T309G polymorphism on development of lung cancer in a Turkish population. Methods: Total 200 subjects including 100 patients and 100 controls were analyzed and used polymerase chain reaction and restriction fragment length polymorphism methods for genotyping analysis of the polymorphism. Results: We found that smokers compared with nonsmokers have approximately eight fold higher lung cancer risk [p = 0.0001, OR = 8.27 (4.02-16.9)]. Frequency of GG genotype was higher in patients than in controls, but this ratio was not significant (chi(2) = 3.5, p = 0.17). Genotype distribution of the polymorphism was not different neither patients with non-small cell lung cancer nor patients with small cell lung cancer (chi(2) = 2.89, p = 0.57). We analyzed together with demographic feature (except smoking habit), clinicopathological findings and genotype frequencies of this polymorphism, and any association with lung cancer risk was not obtained. Conclusion: No correlation between Murine double minute 2 T309G polymorphism and lung cancer risk was detected in this Turkish population.

WOS: 000405114700008, Objective: A genetic polymorphism of SOD1 A251G(rs2070424) is in the 3rd intron region of the SOD gene. The aim of this study was to determine the frequencies of the polymorphisms of the SOD1 A251G in a Turkish population, including 494 healthy individuals. Methods: The 494 Turkish individuals were genotyped for polymorphisms of SOD1 gene. The distribution of SOD1 A251G polymorphisms in this population was examined using a PCR-RFLP method. Genotype and allele frequencies were estimated by counting. Hardy-Weinberg equation between expected and observed genotype distributions was assessed using the X-2 test. Results: In the present study, the distribution of SOD1 A251G polymorphisms in a Turkish population including 494 (females: 278, 56.3% and males: 216, 43.7%) healthy individuals was examined. The mean age of the study population was 38.4 +/- 16.6 years (males, 39.8 +/- 17.1; females, 37.3 +/- 16.1). The observed genotype frequencies of SOD1 A251G were 86.2, 13.4 and 0.4% for AA, AG and GG, respectively. Conclusions: This study provides basic information about the allele and genotype frequency distributions of polymorphisms in the SOD1 A251G gene studied. These frequencies may be useful parameters as a reference for future studies on genetic basis of various diseases and cancer susceptibility.

WOS: 000295543400022, Sulfotransferase 1A1 (SULT1A1) is a member of the sulfotransferase family that plays an important role in the biotransformation of numerous carcinogenic and mutagenic compounds through sulfation. A transition, G to A at position 638, in the SULT1A1 gene, results in the Arg(213)His change. This single nucleotide polymorphism reduces the activity and thermostability of the SULT1A1 enzyme. In the present study, the relationship between the SULT1A1 Arg(213)His polymorphism and prostate cancer was investigated using PCR-RFLP. No significant difference in genotype and allele distribution was noted between the prostate cancer and control populations (P=0.072; P=0.099, respectively). The risk of prostate cancer in individuals carrying the SULT1A1*2 allele (His(213) allele) was determined by combining the SULT1A1*1/SULT1A1*2 (Arg/His(213)) and SULT1A1*2/SULT1A1*2 (His/His(213)) genotypes. No association was observed between SULT1A1 Arg(213)His polymorphism and prostate cancer incidence (P=0.24; OR, 1.36; 95% CI, 0.84-2.25). However, the His(213) allele was found to increase the risk of prostate cancer by 1.36-fold. In smoker and non-smoker populations, no significant relationship was determined between the prostate cancer and control population (P=0.45; P=0.34, respectively)., Research Council of Cumhuriyet University (CUBAP), Sivas, Turkey [F-217], This study was supported by the Research Council of Cumhuriyet University (CUBAP, project no. F-217), Sivas, Turkey.

WOS: 000391387000001, PubMed ID: 27751972, Objective: Mutations in the p53 gene are the most commonly observed genetic abnormalities in malignancies. The purpose of this study was to assess the diagnostic value of serum anti-p53 antibody (Ab) along with the correlation between serum anti-p53 Ab level and quantitative positron emission tomography (PET) parameters such as maximum standardized uptake value (SUVmax), SUVave, metabolic tumor volume, total lesion glycolysis (TLG) and tumor size. Methods: Serum anti-p53 Ab level was studied in three groups. Patients who underwent F-18-fluorodeoxyglucose (FDG) PET/computed tomography (CT) imaging for staging of previously diagnosed lung cancer constituted the first group, while patients who underwent F-18-FDG PET/CT imaging for evaluation of suspicious pulmonary nodules detected on thorax CT and did not show pathologic FDG accumulation (NAPN= pulmonary nodule with non avid-FDG) were enrolled in the second group. The third group consisted of healthy volunteers. Results: Twenty-eight patients with lung cancer (median age: 62.5, range: 39-77years), 28 patients with NAPN (median age: 65, range: 33-79 years), and 24 healthy volunteers (median age: 62, range: 44-74 years) were enrolled in the study. The serum anti-p53 Ab level was low in healthy volunteers while it was higher in both lung cancer patients and NAPN patients (p< 0.05). When serum anti-p53 Ab level and PET parameters were evaluated, there was no significant correlation between serum anti-p53 Ab level and SUVmax, SUVave, TLG, tumor volume and tumor size of patients with lung cancer (p> 0.05). Besides, there was no significant difference between serum anti-p53 Ab level and lesion size of NAPN patients (p> 0.05). Conclusion: It was determined that serum anti-p53 Ab levels are not significantly correlated with PET parameters, and that serum anti-p53 Ab levels increase in any benign or malignant lung parenchyma pathology as compared to healthy volunteers. These results indicate that this Ab cannot be used as a predictor of malignancy in a lung lesion., Cumhuriyet University Scientific Research Project Unit [T-597], The work was supported by grants from Cumhuriyet University Scientific Research Project Unit (T-597).

WOS: 000358306200010, Objective: Manganese superoxide dismutase (MnSOD, SOD2), the only known superoxide scavenger in mitochondria, may be particularly important for antioxidant defense because mitochondria are the major sites for cellular metabolism and hence production of reactive oxygen species. Methods: In this study, 440 Turkish individuals were genotyped for polymorphisms of SOD2 gene. The distribution of these polymorphisms in this population was examined using a PCRRFLP method. Results: In the present study, a total of 440(females: 201, 46% and males: 239, 54%) healthy individuals were studied. The mean age of the study population was 54,41 +/- 5,76 years (males, 55,34 +/- 5,76; females, 53,12 +/- 7,16). The observed genotype frequencies of SOD2 were 17.5, 50.5 and 32.0% for CC, CT and TT, respectively. Conclusion: This study provides basic information about the allele and genotype frequency distributions of polymorphisms of rs4880 in the SOD2 gene studied. These frequencies may be useful parameters as a reference for future studies on genetic basis of various diseases and cancer susceptibility., Scientific Research Project Fund of Cumhuriyet University (CUBAP) [T-403], This work is supported by the Scientific Research Project Fund of Cumhuriyet University (CUBAP) under the project number T-403.

WOS: 000303284700010, To assess the wound healing capabilities of damaged lung tissue in the postpartum period, we investigated the parameters related to wound healing in a rat model of lung damage. Rats were divided into six groups: IA, I B, II, IIIA, IIIB and IV (n=7 in each group). Group IA included rats not in the postpartum period that were sacrificed on the third day after lung injury, group IB included rats not in the postpartum period that were sacrificed on the tenth day after lung injury, group II included rats not in the postpartum period that did not receive lung injury, group IIIA included rats in the postpartum period that were sacrificed on the third day after lung injury, group IIIB included rats in the postpartum period that were sacrificed on the tenth day after lung injury and group IV included rats in the postpartum period without lung injury. Wound healing was evaluated histopathologically and measurements of hydroxyproline levels, serum alanine and glutamine were taken. A significant difference in serum alanine levels was evident between groups IA and IIIA. Significant differences were also observed between serum alanine and glutamine levels in groups IB and IIIB. In conclusion, we demonstrated that serum alanine levels were reduced in the postpartum period following lung injury, which may be expected to negatively impact wound healing in this period. The administration of exogenous alanine for traumatic events occurring during the postpartum period may thus contribute positively to wound healing capabilities during this period., Cumhuriyet University [T-406], This work was supported by the Scientific Research Project Fund of Cumhuriyet University, under project number T-406.

WOS: 000270569200025, PubMed ID: 21475939, Accumulated evidence suggests that alterations due to mutations or genetic polymorphisms in the AXIN2 tumor suppressor gene, a component of the Wnt signaling pathway, contributes to carcinogenesis. The effect of the AXIN2 exon 1 148 C -> T polymorphism was recently investigated in a Japanese population, but has not been investigated in other populations. Additionally, other common polymorphisms of this gene have not been studied. In the present study, 8 polymorphisms of the AXIN2 gene, including exon 1 148 C -> T, were investigated in a Turkish population of 100 lung cancer patients using PCR-RFLP methods. For the exon 1 432 C -> T, exon 5 1365 G -> A, exon 5 1386 C -> T, intron 5 1712+19 G -> T, exon 7 2062 C -> T and intron 7 2141+73 G -> A single nucleotide polymorphisms of AXIN2, no significant association was found between the controls and the lung cancer patients. For exon 1 148 C -> T, a statistically significant association between the controls and lung cancer patients was found. For this region, lung cancer patients with the TT genotype showed a decreased risk [odds ratio (ORTT) 0.33, 95% confidence interval (CI) 0.12-0.89; p=0.032 (adjusted for, gender and smoking status)] as compared with the controls age. with the CC genotype. Concerning histological tumor type, it has been found that exon 1 148 C -> T SNP is associated with a significant decreased risk in squamous cell carcinoma patients (ORTT 0.16; 95% CI 0.03-0.79; p=0.014). Male (ORTT 0.19; 95% CI 0.4-0.77; p=0.015) and smoker (ORTT 0.11; 95% CI 0.01-0.71; p=0.019) lung cancer patients with the TT genotype showed a decreased risk for the same region. Our results suggest that the risk of lung cancer in a Turkish population is related to poly morph isms of the AXIN2 gene., Cumhuriyet University CUBAP, Sivas, Turkey [T-299], This study was supported by Grant no. T-299 from Cumhuriyet University CUBAP, Sivas, Turkey. The technical assistance of Ms. Metal Yilimaz is gratefully acknowledged.

WOS: 000286301100015, PubMed ID: 21461569, Myeloperoxidase (M PO) is a phase I enzyme that can bioactivate many specific procarcinogens, including polycyclic aromatic hydrocarbons and aromatic amines. The MPO gene contains a common single nucleotide polymorphism, for which the -463G>A substitution within the promoter region has been shown to reduce MPO expression and activity. We investigated the association between the MPO -463G>A polymorphism and lung and prostate cancer in a Turkish population. MPO genotypes in the study populations were determined using polymerase chain reaction-based restriction fragment length polymorphism assay. The allelic frequency was significantly different between the cases and controls for lung cancer (p=0.02), but not prostate cancer (p=0.30). No significant difference was noted between the lung and prostate cancer cases and control populations in terms of genotype distribution (p=0.07, p=0.53, respectively). Control groups of lung and prostate cancer were in Hardy-Weinberg equilibrium (p=0.87 and p=0.41, respectively). To determine the protective effect against lung cancer among individuals with the -463A allele, C/A and A/A genotypes were combined. Comparison of the GIG and C/A + A/A genotypes between the lung cancer cases and control groups showed a statistically significant relationship (p=0.032, OR=0.60, 95% CI 0.38-0.95). No gender-specific difference was found in terms of genotype distribution between the lung cancer patients and the controls (female, p=0.20; male, p=0.34). In the case of smokers, a difference in genotype distribution between the lung cancer patients and the controls was statistically significant (p=0.02), although this difference was not statistically significant for non-smokers (p=0.90). Overall, no statistically significant difference was found between the prostate cancer cases and the controls in terms of genotype combination (p=0.46, OR=0.83, 95% CI 0.51-1.36). Additionally, in smokers and non-smokers, no significant relationship was determined between the prostate cancer patients and the control population (p=0.21, p=0.91, respectively). These results suggest that the MPO -463A allele significantly contributes to a protective effect overall and in smokers against lung cancer.

WOS: 000279207000030, PubMed ID: 21472306, Microsomal epoxide hydrolase plays a dual role in the activation and detoxification of carcinogenic compounds. Two polymorphic sites have been described in exons 3 and 4 of the microsomal epoxide hydrolase gene that change tyrosine residue 113 to histidine (Tyr113His) and histidine 139 to arginine (His139Arg), respectively. The exon 3 polymorphism reduces enzyme activity by approximately 50%, whereas the exon 4 polymorphism causes a 25% increase in activity. In the present study, the distribution of these polymorphisms in a Turkish population including 625 unrelated healthy individuals was examined using a PCR-RFLP method. The observed genotype frequencies of microsomal epoxide hydrolase exon 3 were 54,38 and 8% for Tyr113Tyr, Tyr113His and His113His, respectively. Exon 4 genotype frequencies were found to be 69, 29 and 2% for His139His, His139Arg and Arg139Arg, respectively., Cumhuriyet University CU BAP, Sivas, Turkey [T-277], This work was supported by grant no. T-277 from the Cumhuriyet University CU BAP, Sivas, Turkey.