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2. Cellular effects of terahertz waves

Author

Cherkasova, Olga P., primary, Serdyukov, Danil S., additional, Nemova, Eugenia F., additional, Ratushnyak, Alexander S., additional, Kucheryavenko, Anna S., additional, Dolganova, Irina N., additional, Xu, Guofu, additional, Skorobogatiy, Maksim, additional, Reshetov, Igor V., additional, Timashev, Peter S., additional, Spektor, Igor E., additional, Zaytsev, Kirill I., additional, and Tuchin, Valery V., additional

Published
2021
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7. Fluorescent bacterial biosensor E. coli /pTdcR-TurboYFP sensitive to terahertz radiation.

Author

Serdyukov DS, Goryachkovskaya TN, Mescheryakova IA, Kuznetsov SA, Popik VM, and Peltek SE

Abstract

A fluorescent biosensor E. coli /pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli ( E. coli ) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli ., Competing Interests: The authors declare no conflicts of interest., (© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.)

Published
2021
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