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17 results on '"Seifarth, Christof"'

1. Preadipocytes in human granulation tissue: role in wound healing and response to macrophage polarization

Author

Rauchenwald, Tina, Handle, Florian, Connolly, Catherine E., Degen, Antonia, Seifarth, Christof, Hermann, Martin, Tripp, Christoph H., Wilflingseder, Doris, Lobenwein, Susanne, Savic, Dragana, Pölzl, Leo, Morandi, Evi M., Wolfram, Dolores, Skvortsova, Ira-Ida, Stoitzner, Patrizia, Haybaeck, Johannes, Konschake, Marko, Pierer, Gerhard, and Ploner, Christian

Published
2023
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2. Cytotoxic response of tumor-infiltrating lymphocytes of head and neck cancer slice cultures under mitochondrial dysfunction

Author

Greier, Maria do Carmo, primary, Runge, Annette, additional, Dudas, Jozsef, additional, Hartl, Roland, additional, Santer, Matthias, additional, Dejaco, Daniel, additional, Steinbichler, Teresa Bernadette, additional, Federspiel, Julia, additional, Seifarth, Christof, additional, Konschake, Marko, additional, Sprung, Susanne, additional, Sopper, Sieghart, additional, Randhawa, Avneet, additional, Mayr, Melissa, additional, Hofauer, Benedikt Gabriel, additional, and Riechelmann, Herbert, additional

Published
2024
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4. Clinical and histological work-up of vitreoretinal metastases from cutaneous malignant melanoma: A case report

Author

Osl, Antonia, Blatsios, Georgios, Rauchegger, Teresa, Nguyen, Van, Seifarth, Christof, and Haas, Gertrud

Subjects
Eye cancer -- Case studies -- Diagnosis, Skin cancer -- Case studies -- Diagnosis, Metastasis -- Case studies -- Diagnosis, Melanoma -- Case studies -- Diagnosis, Health
Abstract

Byline: Antonia. Osl, Georgios. Blatsios, Teresa. Rauchegger, Van. Nguyen, Christof. Seifarth, Gertrud. Haas We report a rare case of vitreoretinal metastases from cutaneous malignant melanoma (CMM). A 76-year-old patient with [...]

Published
2022

5. High expression of prostate-specific membrane antigen in the tumor-associated neo-vasculature is associated with worse prognosis in squamous cell carcinoma of the oral cavity

Author

Haffner, Michael C, Laimer, Johannes, Chaux, Alcides, Schäfer, Georg, Obrist, Peter, Brunner, Andrea, Kronberger, Irmgard E, Laimer, Klaus, Gurel, Bora, Koller, Johann-Benedikt, Seifarth, Christof, Zelger, Bettina, Klocker, Helmut, Rasse, Michael, Doppler, Wolfgang, and Bander, Neil H

Published
2012
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8. Additional file 1 of Oxidant therapy improves adipogenic differentiation of adipose-derived stem cells in human wound healing

Author

Ploner, Christian, Rauchenwald, Tina, Connolly, Catherine E., Joehrer, Karin, Rainer, Johannes, Seifarth, Christof, Hermann, Martin, Nagl, Markus, Lobenwein, Susanne, Wilflingseder, Doris, Cappellano, Giuseppe, Morandi, Evi M., and Pierer, Gerhard

Abstract

Additional file 1: Supplement Figure 1. Detection of adipocytes in unfixed granulation tissue. Intra-vital confocal microscopy single channel images of unfixed granulation tissue compared with subcutaneous fat tissue (merge displayed in Fig. 1b) stained with BODIPY-493/503 to highlight lipid droplets, wheat germ agglutinin (CF®555 WGA) to highlight ECM substrates and cell morphology (N-acetyl-D-glucosamine and sialic acid present in hyaluronan and other heterogeneous polysaccharides), and Hoechst 33342 staining for nuclei. Supplement Figure 2. PPARG overexpression in day 3 differentiated cells. Representative immunoblotting of PPARG- or puromycin overexpressing human ASC subjected to in vitro differentiation for 3 days in the presence of macrophage-CM. Supplement Figure 3. Numbers of NCT-regulated cytokines. Comparison of cytokines with greater than two-fold change in response to NCT-treatment in various CM. Shown are the total number of regulated cytokines per CM (Set Size), and the number of regulated cytokines found exclusively in one CM or shared between different CM types (Intersection Size). Intersections between different CM types are indicated with connected black dots, single dots indicate a single CM. As shown, 22, 7, 6 and 2 cytokines were found exclusively regulated by M(IFNG/LPS), Mo, M0 and M(IL4/IL13) respectively. Four cytokines were similarly regulated in M(IFNG/LPS) and Mo, 3 in M(IFNG/LPS) and M0, 2 in M0 and M(IL4/IL13), 1 in M(IFNG/LPS) and M(IL4/IL13), and 2 in M(IFNG/LPS), M0 and M(IL4/IL13). Supplement Figure 4. Efficacy of IL1RA to inhibit anti-adipogenic effect of IL1B. IL1B receptor mediated signaling was inhibited by the addition of 100ng/ml recombinant IL1RA. Effect on adipocyte differentiation was evaluated by flow cytometry assessing numbers of lipid-laden cells. Values depict the percentage of lipid-laden adipocytes as a percentage of untreated control cells (0ng IL1B) that emit green fluorescence after staining with BODIPY-493/503, n=3. Statistical significance was determined by using non parametric unpaired Student’s t-test. Data are shown as mean ± SEM. Asterisks indicate p-values < 0.05 (*). Supplement Table 1. Table with log2 cytokine protein expression levels used to generate the heatmap in Fig. 2c. (1) and (2) depict values obtained from two independent measurements. Supplement Table 2. Regulation of cytokines by NCT co-treatment in various CM. Contains log2 fold change (M) and log2 average (A) values representing the extent of regulation, and average expression, of cytokines following NCT co-treatment of CM (e.g. a comparison of NCT co-treated Mo cells against untreated Mo cells). Supplement Table 3. List of Primers used for quantitative RT-PCR.

Published
2021
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9. Somatic mutations throughout the entire mitochondrial genome are associated with elevated PSA levels in prostate cancer patients

Author

Kloss-Brandstatter, Anita, Schafer, Georg, Erhart, Gertraud, Huttenhofer, Alexander, Coassin, Stefan, Seifarth, Christof, Summerer, Monika, Bektic, Jasmin, Klocker, Helmut, and Kronenberg, Florian

Subjects
Prostate cancer -- Genetic aspects, Prostate cancer -- Care and treatment, Gene therapy -- Analysis, Gene mutations -- Analysis, Biological sciences
Abstract

A rigorous high-quality sequencing strategy was used to sequence the entire mitochondrial genome in macrodissected prostate cancer tissue and distant benign cells and analyze frequency and nature of somatic mtDNA mutations and their suitability for monitoring malignant transformation, tumor progression and metastasis. The somatic mutations in transfer RNAs (tRNAs) was found to be associated with elevated prostate specific antigen (PSA) levels, and the degree of heteroplasmy increased with increasing tumor activity and hence somatic mutations in the mitochondrial genome are frequent events in prostate cancer.

Published
2010

10. En-face analysis of the human limbal lymphatic vasculature

Author

Palme, Christoph, Ahmad, Sajjad, Romano, Vito, Seifarth, Christof, Williams, Bryan, Parekh, Mohit, Kaye, Stephen B., Steger, Bernhard, Palme, Christoph, Ahmad, Sajjad, Romano, Vito, Seifarth, Christof, Williams, Bryan, Parekh, Mohit, Kaye, Stephen B., and Steger, Bernhard

Abstract

Purpose: To describe the location and morphometric characteristics of the human limbal lymphatic vasculature and its relation to the marginal corneal vascular arcades (MCA). Methods: Ex vivo confocal microscopic (CM) imaging and immunofluorescence double staining for CD-31 and D2-40 of histological en-face sections using 12 preserved human cadaveric corneoscleral discs were performed, followed by a semi-automated morphometric analysis of the two-dimensional vascular network architecture. Results: Ex vivo CM confirmed the presence of 2 distinct vascular networks. The haematic limbal vascular complex (HLVC) extended further into the cornea, forming typical MCAs. The lymphatic limbal vascular complex (LLVC) was peripheral from the termination of Bowman's layer and was also found to be peripheral to and deeper than the HLVC. LLVC and HLVC were significantly different with respect to vessel diameter, segment length and wall thickness. Conclusion: The lymphatic vasculature of the human corneoscleral limbal region displays specific morphometric features that allow its differentiation from haematic vessels using CM.

Published
2020

12. Systemic counterregulatory response of angiopoietin‐2 after aflibercept therapy for nAMD: a potential escape mechanism.

Author

Angermann, Reinhard, Rauchegger, Teresa, Nowosielski, Yvonne, Seifarth, Christof, Egger, Stefan, Kralinger, Martina T., Kieselbach, Gerhard F., and Zehetner, Claus

Subjects
VASCULAR endothelial growth factors, AFLIBERCEPT, ANGIOPOIETIN-2, INTRAVITREAL injections, ENZYME-linked immunosorbent assay
Abstract

Purpose: To analyse the effect of intravitreal aflibercept injections on systemic angiopoietin‐2 (Ang2) and vascular endothelial growth factor (VEGF)‐A levels in patients with neovascular age‐related macular degeneration (nAMD). Methods: In a prospective, randomized study, aflibercept (2.0 mg/50 µl) or ranibizumab (0.5 mg/50 µl) was administered intravitreally to 38 treatment‐naive patients. Blood samples were taken before, 7 days after, and 28 days after the first intravitreal therapy. Cytokine levels were measured by enzyme‐linked immunosorbent assay. Twenty‐two age‐ and sex‐matched individuals served as controls. Results: At baseline, there were no significant differences of systemic Ang2 and VEGF‐A levels among the treatment and control groups. After intravitreal aflibercept administration, median (interquartile range: IQR) systemic Ang2 was significantly upregulated from 1819 pg/ml (1262–3099) to 2123 pg/ml (1441–3769; p = 0.011) 7 days after the drug injection and remained non‐significantly elevated at 1944 pg/ml (1431–2546 pg/ml; p = 0.653) 28 days after the drug injection. Median (IQR) systemic VEGF‐A levels were significantly reduced from 43 pg/ml (30–57) to 8 pg/ml (8–8; p < 0.0001) 7 days and 16 pg/ml (8–26; p = 0.001) 28 days after the injection in the aflibercept group. There were no significant effects on systemic VEGF‐A and Ang2 levels in the ranibizumab group at any time point following the first injection. Conclusion: In this study, we report significant systemic upregulation of Ang2 after intravitreal aflibercept administration. This counterregulatory response may represent a potential escape mechanism from antiangiogenic therapy. [ABSTRACT FROM AUTHOR]

Published
2021
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14. Validation of Next-Generation Sequencing of Entire Mitochondrial Genomes and the Diversity of Mitochondrial DNA Mutations in Oral Squamous Cell Carcinoma

Author

Kloss-Brandstätter, Anita, primary, Weissensteiner, Hansi, additional, Erhart, Gertraud, additional, Schäfer, Georg, additional, Forer, Lukas, additional, Schönherr, Sebastian, additional, Pacher, Dominic, additional, Seifarth, Christof, additional, Stöckl, Andrea, additional, Fendt, Liane, additional, Sottsas, Irma, additional, Klocker, Helmut, additional, Huck, Christian W., additional, Rasse, Michael, additional, Kronenberg, Florian, additional, and Kloss, Frank R., additional

Published
2015
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15. Enhanced Inhibition of Prostate Tumor Growth by Dual Targeting the Androgen Receptor and the Regulatory Subunit Type Iα of Protein Kinase A in Vivo

Author

Eder, Iris, primary, Egger, Martina, additional, Neuwirt, Hannes, additional, Seifarth, Christof, additional, Maddalo, Danilo, additional, Desiniotis, Andreas, additional, Schäfer, Georg, additional, Puhr, Martin, additional, Bektic, Jasmin, additional, Cato, Andrew, additional, and Klocker, Helmut, additional

Published
2013
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16. Characterization of Transcriptional Changes in ERG Rearrangement-Positive Prostate Cancer Identifies the Regulation of Metabolic Sensors Such as Neuropeptide Y

Author

Massoner, Petra, primary, Kugler, Karl G., additional, Unterberger, Karin, additional, Kuner, Ruprecht, additional, Mueller, Laurin A. J., additional, Fälth, Maria, additional, Schäfer, Georg, additional, Seifarth, Christof, additional, Ecker, Simone, additional, Verdorfer, Irmgard, additional, Graber, Armin, additional, Sültmann, Holger, additional, and Klocker, Helmut, additional

Published
2013
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