1. Anti-Leukemic Effects Induced by Dendritic Cells of Leukemic Origin from Leukemic Blood Samples Are Comparable under Hypoxic vs. Normoxic Conditions.
- Author
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Doraneh-Gard, Fatemeh, Amberger, Daniel Christoph, Amend, Carina, Weinmann, Melanie, Schwepcke, Christoph, Klauer, Lara, Schutti, Olga, Hosseini, Hedayatollah, Krämer, Doris, Rank, Andreas, Schmid, Christoph, and Schmetzer, Helga Maria
- Subjects
IMMUNONUTRITION diet ,RESEARCH funding ,ANTINEOPLASTIC agents ,TUMOR markers ,IN vivo studies ,IMMUNE system ,ACUTE erythroid leukemia ,DENDRITIC cells ,HYPOXEMIA ,IMMUNITY - Abstract
Simple Summary: Dendritic cells are important mediators in the activation of the innate and adoptive immune response. The generation of DC/DC
leu was comparable under physiological hypoxic and normoxic culture conditions, with no significant differences in frequencies of generated DC/DCleu from leukemic cell lines, peripheral blood mononuclear as well as whole blood cells from patients with acute myeloid leukemia. Moreover, the composition of immune cells and anti-leukemic immune activity after T cell enriched mixed lymphocyte culture with patients' blood pretreated vs not pretreated with defined response modifiers (Kits) was improved, particularly under hypoxic conditions. These data show that the generation of DC/DCleu as well as the induction of anti-leukemic immune activating functionality is possible under standard normoxic as well as physiological hypoxic culture conditions ex vivo. Hypoxia can modulate the immune system by affecting the function and activity of immune cells, potentially leading to altered immune responses. This study investigated the generation of leukemia-derived dendritic cells (DCleu) from leukemic blasts and their impact on immune cell activation under hypoxic (5–10% O2 ) compared to normoxic (21% O2 ) conditions using various immunomodulatory Kits. The results revealed that DC/DCleu -generation was similar under hypoxic and normoxic conditions, with no significant differences observed in frequencies of generated DC/DCleu . Furthermore, the study showed that the activation of immune cells and their anti-leukemic activity improved when T cell-enriched immunoreactive cells were co-cultured with DC/DCleu which were generated with Kit I and M compared to the control after mixed lymphocyte cultures. The anti-leukemic activity was improved under hypoxic compared to normoxic conditions after MLCWB-DC Kit M . These findings suggest that DC/DCleu -cultures of leukemic whole blood with Kits under hypoxic conditions yield comparable frequencies of DC/DCleu and can even increase the anti-leukemic activity compared to normoxic conditions. Overall, this research highlights the potential of utilizing DC/DCleu (potentially induced in vivo with Kits) as a promising approach to enhance immune response in patients with acute myeloid leukemia. [ABSTRACT FROM AUTHOR]- Published
- 2024
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