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4. Identification, characterization and full-length sequence analysis of a novel dsRNA virus isolated from the arboreal ant Camponotus yamaokai

Author

Koyama, Satoshi, primary, Sassa, Yukiko, additional, Ono, Shin-ichi, additional, Takata, Mamoru, additional, Furuya, Tetsuya, additional, Satoh, Toshiyuki, additional, Ohmatsu, Tsutomu, additional, Nagai, Makoto, additional, Sakai, Chihiro, additional, Mizutani, Testuya, additional, Hayashi, Shinya, additional, Moriyama, Hiromitsu, additional, and Urayama, Syun-ichi, additional

Published
2015
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5. Quantitative PCR detection of feline morbillivirus in cat urine samples

Author

FURUYA, Tetsuya, primary, WACHI, Akiko, additional, SASSA, Yukiko, additional, OMATSU, Tsutomu, additional, NAGAI, Makoto, additional, FUKUSHIMA, Ryuji, additional, SHIBUTANI, Makoto, additional, YAMAGUCHI, Tomohiro, additional, UEMATSU, Yosuke, additional, SHIROTA, Kinji, additional, and MIZUTANI, Tetsuya, additional

Published
2015
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6. Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing

Author

MINAMI-FUKUDA, Fujiko, primary, NAGAI, Makoto, additional, TAKAI, Hikaru, additional, MURAKAMI, Toshiaki, additional, OZAWA, Tadashi, additional, TSUCHIAKA, Shinobu, additional, OKAZAKI, Sachiko, additional, KATAYAMA, Yukie, additional, OBA, Mami, additional, NISHIURA, Naomi, additional, SASSA, Yukiko, additional, OMATSU, Tsutomu, additional, FURUYA, Tetsuya, additional, KOYAMA, Satoshi, additional, SHIRAI, Junsuke, additional, TSUNEMITSU, Hiroshi, additional, FUJII, Yoshiki, additional, KATAYAMA, Kazuhiko, additional, and MIZUTANI, Tetsuya, additional

Published
2013
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9. Quantitative PCR detection of feline morbillivirus in cat urine samples.

Author

Furuya T, Wachi A, Sassa Y, Omatsu T, Nagai M, Fukushima R, Shibutani M, Yamaguchi T, Uematsu Y, Shirota K, and Mizutani T

Subjects
Animals, Cat Diseases urine, Cats, Morbillivirus Infections urine, Morbillivirus Infections virology, Reverse Transcriptase Polymerase Chain Reaction methods, Cat Diseases virology, Morbillivirus isolation & purification, Morbillivirus Infections veterinary, Reverse Transcriptase Polymerase Chain Reaction veterinary
Abstract

Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.

Published
2016
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