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This study was conducted to prepare a map of the biological properties in the gypsiferouse soil using geomatic techniques at the Tikrit city site. (20) soil samples of the surface layer of gypsum soil were collected randomly according to differently in the nature of agricultural exploitation as survey study. Analyses and measurements of physical (texture and bulk density) and chemical characteristics were carried out: pH, EC, O.M, CaSO4.2H2O and the bacteria and fungi numbers were estimated by methods Certified in the laboratory. Used satellite Images acquired at 24Feb.2019 and 20sep.2019 were used and processed digitally and calculated the following vegetation indicators as follows: NDVI, OSAVI, LAI and LST. Maps of the biological properties (Bacteria and Fungi) were prepared using the Kriging interpolation according to The Spheroidal Model in the ArcGIS Ver environment.10.2. The show results the effect of gypsum content in the bacteria and fungi with the R2 (0.75 and 0.79). The results reached the importance of indices in evaluating the state and activity of bacteria as a positive regression relationship between the NDVI, LAI and OSAVI with the numbers of bacteria and fungi range (0.49-0.80) and were negative regression with the earth’s surface temperature LST. Therefore, spectral indices has proved important in spatial forecasting of the distribution and activity of organisms and the control of seasonal changes in soil surface.