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BACKGROUND Acute Myocardial Infraction (AMI) is a serious and life-threatening manifestation of coronary artery disease (CAD) affecting both males and females, which need immediate management. The present study was aimed to assess the gender difference and factors affecting pre-hospital delay among patients with AMI. MATERIALS AND METHODS The 290 patients admitted with AMI from 13-02-2016 to 26-03-2016 were studied. A descriptive comparative design was adopted for the study. Socio personal and clinical variables of the subjects were assessed. Pre-hospital delay was measured in terms of time from the onset of symptoms till the patient reaches a health care setting. One hour after the onset of symptom of AMI was considered as the Golden hour. Data was analysed using SPSS 20.0 software. RESULTS Out of the 290 patients with AMI (145 males and 145 females), only 32.4% of males and 24.1% of females reached a health care setting within 1 hr of onset of symptoms. 35.2% of male patients and 43.5% of female patients reached the health care setting only after 6 hrs of onset of symptoms. Among the male patients, prehospital delay was significantly associated with place of onset of symptoms (p