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For clarification of a variation character for “high content of β-carotene in fruits” determinant in C. annuum. var. annuum in connection with the genotype, the growing conditions and the interaction between the genes, controlling this determinant, and the genes, controlling the anthocyanins color of vegetative and generative organs the authors analyzed more 100 variants, relating to cultivars Grossum L. (Sendt), Longum D.C., Pomifera Fingerh иFascicullatum Sturt,including the varieties and the hybrids, from own selection. It was shown, that the results of the β-carotene content testing by the GOST 8756.22 and by the high performance liquid chromatography method are correlated (r = 0.99), that is relatively comparable. The intensity of fruits color during the phase of technical ripeness exactly correlates with provitamin A content, however, the fruits color and provitamin A content during the phase of biological ripeness doesn’t interrelated. To obtain the new initial pepper material with a complex of practical valuable determinants, including a high content of provitamin A, ascorbic acid and anthocyanins in fruits, the authors recommend to use the tame mutant forms of pepper of natural and artificial origin with A,Im and bc genes.