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To explore the expression and biological functions of linc00337 in colorectal cancer (CRC), as well as its underlying mechanism.The relative expression of linc00337 in 47 cases of CRC tissues and cells was detected via quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). The si-linc00337 interference sequences were designed and transiently transfected into CRC cells. The interference efficiency was detected via qRT-PCR. Regulatory effect of linc00337 on proliferation of CRC cells was detected via colony formation assay. Cell cycle distribution and apoptosis rate after interference in linc00337 expression were determined using flow cytometry. Moreover, the effects of linc00337 knockdown on cell migration and invasion were detected using transwell assay. At last, the effect of si-linc00337 on the MEK/ERK signaling pathway was detected using Western blotting.The results of qRT-PCR showed that among the 47 cases of CRC tissues, the expression of linc00337 was up-regulated in 40 cases. Similarly, it was highly expressed in CRC cell lines. The results of colony formation assay manifested that cell proliferation declined after interference in linc00337 expression. The results of flow cytometry and transwell assay showed that interference in linc00337 expression arrested the cell cycle in G1/G0 phase, increased the apoptosis rate, and inhibited the invasion and migration of CRC cells. According to the results of Western blotting, expressions of molecular markers in the MEK/ERK pathway after interference in linc00337 expression were significantly changed.Linc00337 is up-regulated in CRC tissues and cells. Interference in linc00337 expression can inhibit cell proliferation, migration, and invasion and promote apoptosis through the MEK/ERK pathway.

Currently, the commercial antimony metallurgy is mainly based on pyrometallurgical processes and oxidative volatilization of Sb2S3 is an essential step. This step includes the problems of high energy consumption and low concentration of SO2 pollution. Aiming at these problems, we present a new method of sulfur-fixing roasting of antimony sulfide. This method uses ZnO as a sulfur-fixing agent, and roasting with Sb2S3 was carried out at 673 K~1073 K to produce Sb2O3 and ZnS. By calculating the thermodynamics of the reactions, we can conclude that the Gibbs Free Energy Change (?G?) of roasting reaction is below -60 kJ/mol and the predominance areas of Sb2O3 and ZnS are wide and right shifting with the temperature increase, which all indicates that this method is theoretically feasible. The reacted products between Sb2S3 and ZnO indicated that the reaction began at 773 K and finished approximately at 973K. We used the Ozawa-Flynn-Wall, Kissinger and Coats-Redfern method to calculate the kinetics of the roasting reaction. The conclusion is as follows: The average values of apparent activation energy (E) and natural logarithmic frequency factor (lnA) calculated by Ozawa-Flynn-Wall, Kissinger and Coats-Redfern were 189.72 kJ?mol-1 and 35.29 s-1, respectively. The mechanism of this reaction was phase boundary reaction model. The kinetic equation is shown as follow, where ? represents reaction fraction: 1-(1-?)1/3 = 2.12 x1015 exp(-1.90x105/RT) t.

Zirconium isotopes have the potential to trace both magmatic differentiation and crustal evolution, as well as deep Earth processes. Zirconium is compatible in bridgmanite where it has a higher coordination number than in silicate melt, implying that Zr isotopes could be fractionated during magma ocean crystallisation. We report the Zr isotopic composition of 31 komatiites from around the globe, ranging in age from 2.41 to 3.55 Ga. The delta Zr-94/90 (per mille deviation of Zr-94/Zr-90 from IPGP-Zr standard) values for the komatiites are homogeneous at 0.030 +/- 0.049 parts per thousand (all errors are 2 s.d.), and consistent with associated basaltic rocks (0.048 +/- 0.032 parts per thousand). These results, identical within uncertainty of the bulk silicate Earth estimate from modern basalts, suggest that the mantle Zr isotope composition has been constant since at least 3.55 Ga. Combining the isotopic compositions of komatiites and basalts of all ages we suggest a conservative delta Zr-94/90 = 0.040 +/- 0.044 parts per thousand (n = 72) for the mantle composition. Several komatiite systems that we analysed in this study, including Schapenburg, Komati, and Weltevreden, have been previously argued to have isotope signatures consistent with magma ocean crystallisation processes. However, their Zr isotope compositions are indistinguishable from other komatiites, implying that bridgmanite crystallisation did not fractionate Zr isotopes to any measurable extent.

Interfacial exchange coupling and magnetization reversal characteristics in the perpendicular heterostructures consisting of an amorphous ferrimagnetic (FI) TbxCo100–x alloy layer exchange-coupled with a ferromagnetic (FM) [Co/Ni]N multilayer have been investigated. As compared with pure TbxCo100–x alloy, the magnetization compensation composition of the heterostructures shift to a higher Tb content, implying Co/Ni also serves to compensate the Tb moment in TbCo layer. The net magnetization switching field Hc⊥ and interlayer interfacial coupling field Hex, are not only sensitive to the magnetization and thickness of the switched TbxCo100–x or [Co/Ni]N layer, but also to the perpendicular magnetic anisotropy strength of the pinning layer. By tuning the layer structure we achieve simultaneously both large Hc⊥ = 1.31 T and Hex = 2.19 T. These results, in addition to the fundamental interest, are important to understanding of the interfacial coupling interaction in the FM/FI heterostructures, which could offer the guiding of potential applications in heat-assisted magnetic recording or all-optical switching recording technique.

In order to understand the pollution characteristics of polycyclic aromatic hydrocarbons (PAHs) in west Bohai Bay, China, the sixteen PAHs listed by US EPA in surface seawater were investigated in the Qinhuangdao (QHD), Caofeidian (CFD) and Huanghuagang (HHG) areas. Distribution and composition of PAHs in the dissolved phase (DP) and the suspended particulate associated phase (SP) were detected by gas chromatography and mass spectrometry (GC-MS), respectively. An ecological risk assessment was evaluated using risk quotients (RQ). The results showed that the 16 PAHs were prevalent in west Bohai Bay and the 2-3 rings PAHs were the predominant chemicals. The QHD area had the highest concentration of total PAHs (TPAHs), which ranged from 186.3 to 531.3 ng/L in DP and 222.7 to 715.7 ng/L in SPM. In all three areas, the PAHs levels in SPM were higher than those in DP. The risk assessment showed that there was a relatively low potential ecological risk of PAHs in the coastal regions of Bohai Bay.

In order to understand the risk of polybrominated diphenyl ethers (PBDEs) existing in sediment for years, the accumulation of PBDEs in sediment aged for 2 years to fish was investigated. Simulated aquatic system microcosms were conducted with PBDE contaminated sediment aged for 2 years and carps were cultured in the microcosms for 20 days. PBDE concentrations in carp tissues were analyzed to estimate the bioavailability of aged PBDEs in carps. The main spiked PBDE congeners were detected in sediment even though the contaminated sediment was aged for 2 years. Similarly, the five PBDE (BDE-28, 47, 100, 153 and 154) congeners which probably were bioaccumulated by carp were detected in fish tissues, indicating that PBDEs could be bioaccumulated after aging for 2 years. The PBDEs distribution revealed that the concentrations of polybrominated diphenyl ethers in tissues of Cyprinus carpio is in this order of magnitude: gut > liver > gill > fillet. The PBDEs concentrations in fillet were as high as 67.9 ng/g dry wt, in which BDE-47 contributed almost 50% in profile.