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2 results on '"Romero-Cano R"'

1. Evaluation of the inflammatory responses to sol-gel coatings with distinct biocompatibility levels

Author

Cerqueira, A., Araújo-Gomes, N., Zhang, Y., Beucken, J.J. van den, Martínez-Ramos, C., Ozturan, S., Izquierdo, R., Muriach, M., Romero-Cano, R., Baliño, P., Romero-Gavilán, F.J., Cerqueira, A., Araújo-Gomes, N., Zhang, Y., Beucken, J.J. van den, Martínez-Ramos, C., Ozturan, S., Izquierdo, R., Muriach, M., Romero-Cano, R., Baliño, P., and Romero-Gavilán, F.J.

Abstract

Item does not contain fulltext, The immune system plays a crucial role in determining the implantation outcome, and macrophages are in the frontline of the inflammatory processes. Further, cellular oxidative stress resulting from the material recognition can influence how cell responses develop. Considering this, the aim of this study was to study oxidative stress and macrophages phenotypes in response to sol-gel materials with distinct in vivo outcomes. Four materials were selected (70M30T and 35M35G30T, with high biocompatibility, and 50M50G and 50V50G, with low biocompatibility). Gene expression, immunocytochemistry and cytokine secretion profiles for M1 and M2 markers were determined. Moreover, oxidative stress markers were studied. Immunocytochemistry and ELISA showed that 50M50G and 50V50G lead to a higher differentiation to M1 phenotype, while 70M30T and 35M35G30T promoted M2 differentiation. In oxidative stress, no differences were found. These results show that the balance between M1 and M2, more than individual quantification of each phenotype, determines a biomaterial outcome.

Published
2021

2. Biochemical and Behavioral Consequences of Ethanol Intake in a Mouse Model of Metabolic Syndrome.

Author

Baliño P, Romero-Cano R, and Muriach M

Subjects
Animals, Antioxidants metabolism, Blood Glucose metabolism, Brain drug effects, Brain metabolism, Central Nervous System Depressants administration & dosage, Central Nervous System Depressants pharmacology, Ethanol administration & dosage, Glutathione metabolism, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Humans, Insulin blood, Insulin metabolism, Male, Malondialdehyde metabolism, Metabolic Syndrome blood, Metabolic Syndrome genetics, Mice, Inbred C57BL, Mice, Knockout, Obesity blood, Obesity genetics, Obesity metabolism, Receptors, Leptin genetics, Mice, Disease Models, Animal, Ethanol pharmacology, Metabolic Syndrome metabolism, Receptors, Leptin deficiency
Abstract

Ethanol abuse is a common issue in individuals with sedentary lifestyles, unbalanced diets, and metabolic syndrome. Both ethanol abuse and metabolic syndrome have negative impacts on the central nervous system, with effects including cognitive impairment and brain oxidative status deterioration. The combined effects of ethanol abuse and metabolic syndrome at a central level have not yet been elucidated in detail. Thus, this work aims to determine the effects of ethanol intake on a mouse model of metabolic syndrome at the behavioral and biochemical levels. Seven-week-old male control (B6.V-Lep ob/+JRj) and leptin-deficient (metabolic syndrome) (B6.V-Lep ob/obJRj) mice were used in the study. Animals were divided into four groups: control, ethanol, obese, and obese-ethanol. Ethanol consumption was monitored for 6 weeks. Basal glycemia, insulin, and glucose overload tests were performed. To assess short- and long-term memory, an object recognition test was used. In order to assess oxidative status in mouse brain samples, antioxidant enzyme activity was analyzed with regard to glutathione peroxidase, glutathione reductase, glutathione, glutathione disulfide, lipid peroxidation products, and malondialdehyde. Ethanol intake modulated the insulin response and impaired the oxidative status in the ob mouse brain.

Published
2021
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