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9. Diversity in lacOperon Regulation among Diverse Escherichia coliIsolates Depends on the Broader Genetic Background but Is Not Explained by Genetic Relatedness

Author

Phillips, Kelly N., Widmann, Scott, Lai, Huei-Yi, Nguyen, Jennifer, Ray, J. Christian J., Balázsi, Gábor, and Cooper, Tim F.

Abstract

The lacoperon of Escherichia coliis a classic model for studying gene regulation. This study has uncovered features such as the environmental input logic controlling gene expression, as well as gene expression bistability and hysteresis. Most lacoperon studies have focused on a few lab strains, and it is not known how generally those findings apply to the diversity of E. colistrains. We examined the environmental dependence of lacgene regulation in 20 natural isolates of E. coliand found a wide range of regulatory responses. By transferring lacgenes from natural isolate strains into a common reference strain, we found that regulation depends on both the lacgenes themselves and on the broader genetic background, indicating potential for still-greater regulatory diversity following horizontal gene transfer. Our results reveal that there is substantial natural variation in the regulation of the lacoperon and indicate that this variation can be ecologically meaningful.

Published
2019
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