48 results on '"Rønnestad, I."'
Search Results
2. Feeding behaviour and digestive physiology in larval fish: Current knowledge, and gaps and bottlenecks in research
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Rønnestad, I, Yúfera, M, Ueberschär, B, Ribeiro, L, Sæle, Ø, Izquierdo, M, and Boglione, C
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Settore BIO/07 ,media_common.quotation_subject ,Management, Monitoring, Policy and Law ,Aquatic Science ,Biology ,Predation ,Absorption ,03 medical and health sciences ,Nutrient ,Aquaculture ,Gut ,14. Life underwater ,absorption, digestion, gut, ingestion ,030304 developmental biology ,media_common ,0303 health sciences ,Ecology ,business.industry ,Ingestion ,fungi ,Appetite ,04 agricultural and veterinary sciences ,Ichthyoplankton ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Digestion ,Digestive functions ,Adaptation ,business - Abstract
Food uptake follows rules defined by feeding behaviour that determines the kind and quantity of food ingested by fish larvae as well as how live prey and food particles are detected, captured and ingested. Feeding success depends on the progressive development of anatomical characteristics and physiological functions and on the availability of suitable food items throughout larval development. The fish larval stages present eco-morpho-physiological features very different from adults and differ from one species to another. The organoleptic properties, dimensions, detectability, movements characteristics and buoyancy of food items are all crucial features that should be considered, but is often ignored, in feeding regimes. Ontogenetic changes in digestive function lead to limitations in the ability to process certain feedstuffs. There is still a lack of knowledge about the digestion and absorption of various nutrients and about the ontogeny of basic physiological mechanisms in fish larvae, including how they are affected by genetic, dietary and environmental factors. The neural and hormonal regulation of the digestive process and of appetite is critical for optimizing digestion. These processes are still poorly described in fish larvae and attempts to develop optimal feeding regimes are often still on a 'trial and error' basis. A holistic understanding of feeding ecology and digestive functions is important for designing diets for fish larvae and the adaptation of rearing conditions to meet requirements for the best presentation of prey and microdiets, and their optimal ingestion, digestion and absorption. More research that targets gaps in our knowledge should advance larval rearing. © 2013 Wiley Publishing Asia Pty Ltd., This study benefited from participation in LARVANET – COST action FA0801 (EU RTD framework Programme). I.R. received funding from the Research Council of Norway (CODE- 199482 and GutFeeling- 190019) and the EC FP7 (LIFECYCLE- 222719). M.Y. received financial support from the Spanish Ministry of Science and Innovation MICINN + FEDER/ERDF (projects AGL2007-64450-C02-01 and Consolider Ingenio 2010 Program-project Aquagenomics CSD2007-0002). C.B. received funding from the Italian Ministry of Agricultural, Forestry and Alimentary Policy (Law 41/82) (projects 5C49- 6C138).
- Published
- 2013
3. Fish larval nutrition and feed formulation: Knowledge gaps and bottlenecks for advances in larval rearing (a Larvanet review)
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Hamre, K, Yufera, M, Conceição, L, Rønnestad, I, Boglione, C, and Izquierdo, M
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Settore AGR/19 - Zootecnica Speciale ,Settore BIO/07 ,Settore AGR/20 - Zoocolture ,Settore AGR/18 - Nutrizione e Alimentazione Animale - Abstract
Trabajo presentado en el World Aquaculture 2012, celebrado en Praga del 1 al 5 de septiembre de 2012.
- Published
- 2012
4. Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae
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Vareilles, Mahaut, Richard, Nadège, Gavaia, Paulo J., Silva, Tomé S., Cordeiro, O., Guerreiro, Inês, Yúfera, M., Batista, I., Pires, C., Pousão-Ferreira, P., Rodrigues, Pedro, Rønnestad, I., Fladmark, K. E., and Conceição, L. E. C.
- Subjects
fungi - Abstract
In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co-fed from first-feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP-90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in
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- 2012
5. Fish Larval Research: a tool for sustainable food production and understanding environmental impacts on developing organisms
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Pittman, K., Rønnestad, I., Gavaia, Paulo J., Cancela, Leonor, Guerreiro, P. M., Ribeiro, Laura, Aragão, C., Hamre, K., Moren, M., Yúfera, M., and Conceição, L. E. C.
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fungi - Abstract
Reliable juvenile production or recruitment requires high numbers of healthy fish larvae. Despite considerable progress in marine fish farming in the past 20 years, juvenile fish production is still fraught with problems which arise during the larval phase. In fisheries, juvenile recruitment in some populations has not recovered despite long-term moratoria on captures and protection of the broodstock. These issues highlight the growing importance of multidisciplinary fish larval research.
- Published
- 2007
6. PepT1 in a coldwater marine teleost larvae- Atlantic cod: cloning and preliminary studies of expression and phylogeny
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Rønnestad, I., Gavaia, Paulo J., S B Viegas, Carla, Verri, T., and Cancela, Leonor
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hormones, hormone substitutes, and hormone antagonists - Abstract
Submitted by Paulo Gavaia (pgavaia@ualg.pt) on 2014-06-06T09:52:10Z No. of bitstreams: 1 PepT1 in a coldwater marine teleost larvae atlantic cod cloning and preliminary studies of expression and phylogeny.pdf: 92089 bytes, checksum: 29d7c838f8ab20cd979bf7fa808abca9 (MD5) Approved for entry into archive by Pedro Sousa (a19661@ualg.pt) on 2014-06-25T12:43:54Z (GMT) No. of bitstreams: 1 PepT1 in a coldwater marine teleost larvae atlantic cod cloning and preliminary studies of expression and phylogeny.pdf: 92089 bytes, checksum: 29d7c838f8ab20cd979bf7fa808abca9 (MD5) Made available in DSpace on 2014-06-25T12:43:54Z (GMT). No. of bitstreams: 1 PepT1 in a coldwater marine teleost larvae atlantic cod cloning and preliminary studies of expression and phylogeny.pdf: 92089 bytes, checksum: 29d7c838f8ab20cd979bf7fa808abca9 (MD5) Previous issue date: 2006
- Published
- 2006
7. Cloning and ontogenetic expression of the oligopeptide transporter PepT1 (Solute carrier family 15, member 1), in fed and starved larval Atlantic cod, Gadus morhua L
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Rønnestad, I., Gavaia, Paulo J., S B Viegas, Carla, and Cancela, Leonor
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Functional characteristics of marine fish larvae digestive system at the onset of exogenous feeding have long been a subject for discussion. Absorption of digested proteins in vertebrates has been shown to include transporter systems for single amino acids and small peptides.
- Published
- 2004
8. Expression of the oligopeptide transporter PepT1 (Solute carrier family 15, member 1), in fed and starved larval Zebra fish (Danio rerio)
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Brito, A. B., Rønnestad, I., Gavaia, Paulo J., S B Viegas, Carla, and Cancela, Leonor
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animal structures ,digestive, oral, and skin physiology ,embryonic structures - Abstract
Fish embryos develop while utilizing yolk nutrients supplied by the (...). There normally exists a "first feeding window" between when the larvae (...) able to ingest exogenous feed,and the "point of no return" where (...) exhausted its yolk reserves and also irreversibly degraded critical tissues (...) energetic purposes it feeds is not ingested.
- Published
- 2004
9. Characterisation of two vitellogenins in the salmon louse Lepeophtheirus salmonis: molecular, functional and evolutional analysis
- Author
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Dalvin, S, primary, Frost, P, additional, Loeffen, P, additional, Skern-Mauritzen, R, additional, Baban, J, additional, Rønnestad, I, additional, and Nilsen, F, additional
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- 2011
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10. Pre-digestion of dietary lipids has only minor effects on absorption, retention and metabolism in larval stages of Atlantic cod (Gadus morhua)
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Hamre, K., primary, Lukram, I. M., additional, Rønnestad, I., additional, Nordgreen, A., additional, and Sæle, Ø., additional
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- 2010
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11. Fuel and metabolic scaling during the early life stages of Atlantic cod Gadus morhua
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Finn, RN, primary, Rønnestad, I, additional, van der Meeren, T, additional, and Fyhn, HJ, additional
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- 2002
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12. Utilization of free amino acids related to energy metabolism of developing eggs and larvae of lemon sole Microslomus kitt reared in the laboratory
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Rønnestad, I, primary, Finn, RN, additional, Groot, EP, additional, and Fyhn, HJ, additional
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- 1992
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13. Pre-digestion of dietary lipids has only minor effects on absorption, retention and metabolism in larval stages of Atlantic cod ( Gadus morhua)
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Hamre, K, Lukram, I M, Rønnestad, I, Nordgreen, A, and Sæle, O
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- 2011
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14. Salmo salar oligopeptide transporters PepT1a and PepT1b: a comparative electrophysiological characterization of partial and complete transport cycle
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F. Vacca, A. Gomes, R. Cinquetti, K. Murashita, F. Imperiali, A. Barca, T. Verri, I. Rønnestad, E. Bossi, Vacca, F., Gomes, A., Cinquetti, R., Murashita, K., Imperiali, F., Barca, A., Verri, T., Rønnestad, I., and Bossi, E.
- Abstract
The H+-coupled peptide transporter 1 (PepT1) belongs to SoLute Carrier family 15 (SLC15A1) and is responsible for the absorption of di/tripeptides in enterocytes. Beside its nutritional role, it has been hypothesized that PepT1 functions as a transceptor, i.e. a peptide sensor/transporter involved in gut hormone release from entero-endocrine cell(s)1, 2. Studying the role of PepT1 in peptide absorption in the gut is relevant for the direct relation between dietary protein availability and fish growth3. In salmonids, PepT1 gene has been duplicated and two transporters, i.e. PepT1a and PepT1b, have been found in the intestine. The partial and complete transport cycle of the two PepT1-type transporters of Salmo salar, ssPepT1a and ssPepT1b, heterologously expressed in Xenopus laevis oocytes, were studied using Two Electrode Voltage Clamp technique. The pre-steady state currents of ssPepT1b were like that of other fish orthologs4 but differed from those of ssPepT1a. ssPepT1a showed a slower decaying currents, and the charge vs voltage (Q/V) and time constant vs voltage (τ/V) curves shifted to more positive potentials behaving as the mammalian transporter4. In both transporters, reducing external pH from 7.6 to 6.5 slowed the transients decay, shifting to more positive potential the Q/V and τ/V curves (Fig. 1). To evaluate the transport activity of ssPepT1a and ssPepT1b, the transport-associated currents were recorded in presence of 1mM of lysine(Lys)-containing peptides (KcPeps) as Lys is a limiting amino acid for animal growth3. KcPeps elicited transport-associated currents of different amplitudes, for e.g. ssPepT1b generated large currents when exposed to peptides carrying Lys in the N-terminus (KG, KM), while ssPepT1a produced small currents independently of Lys position (Fig. 2). The current vs voltage (I/V), in the presence of KcPeps, showed small and similar currents at two different pH (6.5, 7.6) in ssPepT1a. Conversely, in ssPepT1b the I/V curves differed at the most negative potentials with larger currents recorded at pH 7.6. KG dose-response experiments were also conducted, and while fitting with logistic equation allowed to obtain the kinetic parameters (K0.5 and Imax) at each voltage for ssPepT1b, only an estimation was possible for ssPepT1a at -140 and -120mV. The analysis on the transient and transport currents indicated important functional differences between ssPepT1a and ssPepT1b transporters. The dissimilar substrate specificity for KcPeps supports the idea of distinct roles in peptide recognition and transport for ssPepT1a and ssPepT1b.
- Published
- 2018
15. The teleost fish PepT1-type peptide transporters and their relationships with neutral and charged substrates.
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Vacca F, Gomes AS, De Gennaro M, Rønnestad I, Bossi E, and Verri T
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In teleosts, two PepT1-type (Slc15a1) transporters, i.e., PepT1a and PepT1b, are expressed at the intestinal level. They translocate charged di/tripeptides with different efficiency, which depends on the position of the charged amino acid in the peptide and the external pH. The relation between the position of the charged amino acid and the capability of transporting the dipeptide was investigated in the zebrafish and Atlantic salmon PepT1-type transporters. Using selected charged (at physiological pH) dipeptides: i.e., the negatively charged Asp-Gly and Gly-Asp, and the positively charged Lys-Gly and Gly-Lys and Lys-Met and Met-Lys, transport currents and kinetic parameters were collected. The neutral dipeptide Gly-Gln was used as a reference substrate. Atlantic salmon PepT1a and PepT1b transport currents were similar in the presence of Asp-Gly and Gly-Asp, while zebrafish PepT1a elicited currents strongly dependent on the position of Asp in the dipeptide and zebrafish PepT1b elicited small transport currents. For Lys- and Met-containing dipeptides smaller currents compared to Gly-Gln were observed in PepT1a-type transporters. In general, for zebrafish PepT1a the currents elicited by all tested substrates slightly increased with membrane potential and pH. For Atlantic salmon PepT1a, the transport current increased with negative potential but only in the presence of Met-containing dipeptides and in a pH-dependent way. Conversely, large currents were shown for PepT1b for all tested substrates but Gly-Lys in Atlantic salmon. This shows that in Atlantic salmon PepT1b for Lys-containing substrates the position of the charged dipeptides carrying the Lys residue defines the current amplitudes, with larger currents observed for Lys in the N-terminal position. Our results add information on the ability of PepT1 to transport charged amino acids and show species-specificity in the kinetic behavior of PepT1-type proteins. They also suggest the importance of the proximity of the substrate binding site of residues such as Lys
PepT1a /GlnPepT1b for recognition and specificity of the charged dipeptide and point out the role of the comparative approach that exploits the natural protein variants to understand the structure and functions of membrane transporters., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Vacca, Gomes, De Gennaro, Rønnestad, Bossi and Verri.)- Published
- 2023
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16. Taurine Supplementation to Plant-Based Diets Improves Lipid Metabolism in Senegalese Sole.
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Aragão C, Teodósio R, Colen R, Richard N, Rønnestad I, Dias J, Conceição LEC, and Ribeiro L
- Abstract
Taurine is a sulphur-containing amino acid with important physiological roles and a key compound for the synthesis of bile salts, which are essential for the emulsion and absorption of dietary lipids. This study aimed to evaluate the effects of taurine supplementation to low-fishmeal diets on the metabolism of taurine, bile acids, and lipids of Senegalese sole. A fishmeal (FM) and a plant-protein-based (PP0) diet were formulated, and the latter was supplemented with taurine at 0.5 and 1.5% (diets PP0.5 and PP1.5). Diets were assigned to triplicate tanks containing 35 fish (initial weight ~14 g) for 6 weeks. Fish from the PP0 treatment presented lower taurine and bile-acid concentrations compared with the FM treatment, and a downregulation of cyp7a1 and abcb11 was observed. Triolein catabolism decreased in PP0-fed fish, resulting in increased hepatic fat content and plasma triglycerides, while no effects on plasma cholesterol were observed. Taurine supplementation to plant-based diets resulted in a higher taurine accumulation in fish tissues, increased bile-acid concentration, and upregulation of cyp7a1 and abcb11 . Hepatic fat content and plasma triglycerides decreased with increasing dietary taurine supplementation. Taurine supplementation mitigated part of the negative effects of plant-based diets, leading to better lipid utilisation.
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- 2023
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17. Light conditions during Atlantic salmon embryogenesis affect key neuropeptides in the melanocortin system during transition from endogenous to exogenous feeding.
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Norland S, Gomes AS, Rønnestad I, Helvik JV, and Eilertsen M
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During the first feeding period, fish will adapt to exogenous feeding as their endogenous source of nutrients is depleted. This requires the development of a functional physiological system to control active search for food, appetite, and food intake. The Atlantic salmon ( Salmo salar ) melanocortin system, a key player in appetite control, includes neuronal circuits expressing neuropeptide y ( npya ), agouti-related peptide ( agrp1 ), cocaine- and amphetamine-regulated transcript ( cart ), and proopiomelanocortin ( pomca ). Little is known about the ontogeny and function of the melanocortin system during early developmental stages. Atlantic salmon [0-730 day degrees (dd)] were reared under three different light conditions (DD, continuous darkness; LD, 14:10 Light: Dark; LL, continuous light) before the light was switched to LD and the fish fed twice a day. We examined the effects of different light conditions (DD
LD , LDLD , and LLLD ) on salmon growth, yolk utilization, and periprandial responses of the neuropeptides npya1, npya2, agrp1, cart2a, cart2b, cart4, pomca1 , and pomca2 . Fish were collected 1 week (alevins, 830 dd, still containing yolk sac) and 3 weeks (fry, 991 dd, yolk sac fully consumed) into the first feeding period and sampled before (-1 h) and after (0.5, 1.5, 3, and 6 h) the first meal of the day. Atlantic salmon reared under DDLD , LDLD , and LLLD had similar standard lengths and myotome heights at the onset of first feeding. However, salmon kept under a constant light condition during endogenous feeding (DDLD and LLLD ) had less yolk at first feeding. At 830 dd none of the neuropeptides analyzed displayed a periprandial response. But 2 weeks later, and with no yolk remaining, significant periprandial changes were observed for npya1, pomca1 , and pomca2 , but only in the LDLD fish. This suggests that these key neuropeptides serve an important role in controlling feeding once Atlantic salmon need to rely entirely on active search and ingestion of exogenous food. Moreover, light conditions during early development did not affect the size of salmon at first feeding but did affect the mRNA levels of npya1, pomca1 , and pomca2 in the brain indicating that mimicking natural light conditions (LDLD ) better stimulates appetite control., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Norland, Gomes, Rønnestad, Helvik and Eilertsen.)- Published
- 2023
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18. Developmental stages of the ballan wrasse from first feeding through metamorphosis: Cranial ossification and the digestive system.
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Norland S, Saele Ø, and Rønnestad I
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- Animals, Metamorphosis, Biological, Mouth, Osteogenesis, Skull, Perciformes
- Abstract
We have described six developmental stages for the ballan wrasse, from the first feeding until the juvenile stage, supported by specific descriptions of cranial ossification, maturation of the digestive tract, and growth-correlated stages. The initial formation and development of bones are closely linked to the functional anatomical structures required for the mechanics of its feeding behavior and ingestion, particularly the jaws and branchial regions involved in opening the mouth and capturing food particles. The overall ontogeny of the cranial structure compares to that of other teleosts. The cranial ossification of the ballan wrasse skull and the development of its dentary apparatus-first pharyngal teeth and later oral teeth-is linked to the development of the digestive system and to their feeding habits, from preying on zooplankton to feeding on crustaceans and invertebrates on rocks and other substrates. As ballan wrasse is a nibbler, eating small meals, the digestive tract is short compared to the length of the fish; there is no stomach or peptic digestion and also no distinctive bulbus and pyloric ceca. The liver and exocrine pancreas and their outlets terminating in the lumen of the most anterior part of the intestine are important in the digestive process and develop with a larger volume than that in gastric teleosts, relative to the digestive system., (© 2022 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.)
- Published
- 2022
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19. Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters.
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Vacca F, Gomes AS, Murashita K, Cinquetti R, Roseti C, Barca A, Rønnestad I, Verri T, and Bossi E
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- Animals, Kinetics, Mammals metabolism, Oocytes metabolism, Rats, Zebrafish genetics, Salmo salar genetics, Salmo salar metabolism, Symporters genetics, Symporters metabolism
- Abstract
The high-affinity/low-capacity system Slc15a2 (PepT2) is responsible for the reuptake of di/tripeptides from the renal proximal tubule, but it also operates in many other tissues and organs. Information regarding PepT2 in teleost fish is limited and, to date, functional data are available from the zebrafish (Danio rerio) only. Here, we report the identification of two slc15a2 genes in the Atlantic salmon (Salmo salar) genome, namely slc15a2a and slc15a2b. The two encoded PepT2 proteins share 87% identity and resemble both structurally and functionally the canonical vertebrate PepT2 system. The mRNA tissue distribution analyses reveal a widespread distribution of slc15a2a transcripts, being more abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and the distal part of the gastrointestinal tract. The function of the two transporters was investigated by heterologous expression in Xenopus laevis oocytes and two-electrode voltage-clamp recordings of transport and presteady-state currents. Both PepT2a and PepT2b in the presence of Gly-Gln elicit pH-dependent and Na
+ independent inward currents. The biophysical and kinetic analysis of the recorded currents defined the transport properties, confirming that the two Atlantic salmon PepT2 proteins behave as high-affinity/low-capacity transporters. The recent structures and the previous kinetic schemes of rat and human PepT2 qualitatively account for the characteristics of the two Atlantic salmon proteins. This study is the first to report on the functional expression of two PepT2-type transporters that operate in the same vertebrate organism as a result of (a) gene duplication process(es). KEY POINTS: Two slc15a2-type genes, slc15a2a and slc15a2b coding for PepT2-type peptide transporters were found in the Atlantic salmon. slc15a2a transcripts, widely distributed in the fish tissues, are abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and distal gastrointestinal tract. Amino acids involved in vertebrate Slc15 transport function are conserved in PepT2a and PepT2b proteins. Detailed kinetic analysis indicates that both PepT2a and PepT2b operate as high-affinity transporters. The kinetic schemes and structures proposed for the mammalian models of PepT2 are suitable to explain the function of the two Atlantic salmon transporters., (© 2022 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.)- Published
- 2022
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20. Regional Expression of npy mRNA Paralogs in the Brain of Atlantic Salmon ( Salmo salar , L.) and Response to Fasting.
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Tolås I, Kalananthan T, Gomes AS, Lai F, Norland S, Murashita K, and Rønnestad I
- Abstract
Neuropeptide Y (NPY) is known as a potent orexigenic signal in vertebrates, but its role in Atlantic salmon has not yet been fully established. In this study, we identified three npy paralogs, named npya1, npya2, and npyb , in the Atlantic salmon genome. In silico analysis revealed that these genes are well conserved across the vertebrate's lineage and the mature peptide sequences shared at least 77% of identity with the human homolog. We analyzed mRNA expression of npy paralogs in eight brain regions of Atlantic salmon post-smolt, and the effect of 4 days of fasting on the npy expression level. Results show that npya1 was the most abundant paralog, and was predominantly expressed in the telencephalon, followed by the midbrain and olfactory bulb. npya2 mRNA was highly abundant in hypothalamus and midbrain, while npyb was found to be highest expressed in the telencephalon, with low mRNA expression levels detected in all the other brain regions. 4 days of fasting resulted in a significant ( p < 0.05) decrease of npya1 mRNA expression in the olfactory bulb, increased npya2 mRNA expression in the midbrain and decreased npyb mRNA expression in the pituitary. In the hypothalamus, the vertebrate appetite center, expression of the npy paralogs was not significantly affected by feeding status. However, we observed a trend of increased npya2 mRNA expression ( p = 0.099) following 4 days of fasting. Altogether, our findings provide a solid basis for further research on appetite and energy metabolism in Atlantic salmon., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Tolås, Kalananthan, Gomes, Lai, Norland, Murashita and Rønnestad.)
- Published
- 2021
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21. Effects of Short-Term Fasting on mRNA Expression of Ghrelin and the Peptide Transporters PepT1 and 2 in Atlantic Salmon ( Salmo salar ).
- Author
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Del Vecchio G, Lai F, Gomes AS, Verri T, Kalananthan T, Barca A, Handeland S, and Rønnestad I
- Abstract
Food intake is a vital process that supplies necessary energy and essential nutrients to the body. Information regarding luminal composition in the gastrointestinal tract (GIT) collected through mechanical and nutrient sensing mechanisms are generally conveyed, in both mammals and fish, to the hypothalamic neurocircuits. In this context, ghrelin, the only known hormone with an orexigenic action, and the intestinal peptide transporters 1 and 2, involved in absorption of dietary di- and tripeptides, exert important and also integrated roles for the nutrient uptake. Together, both are potentially involved in signaling pathways that control food intake originating from different segments of the GIT. However, little is known about the role of different paralogs and their response to fasting. Therefore, after 3 weeks of acclimatization, 12 Atlantic salmon ( Salmo salar ) post-smolt were fasted for 4 days to explore the gastrointestinal response in comparison with fed control ( n = 12). The analysis covered morphometric (weight, length, condition factor, and wet content/weight fish %), molecular (gene expression variations), and correlation analyses. Such short-term fasting is a common and recommended practice used prior to any handling in commercial culture of the species. There were no statistical differences in length and weight but a significant lower condition factor in the fasted group. Transcriptional analysis along the gastrointestinal segments revealed a tendency of downregulation for both paralogous genes slc15a1a and slc15a1b and with significant lowered levels in the pyloric ceca for slc15a1a and in the pyloric ceca and midgut for slc15a1b . No differences were found for slc15a2a and slc15a2b (except a higher expression of the fasted group in the anterior midgut), supporting different roles for slc15 paralogs. This represents the first report on the effects of fasting on slc15a2 expressed in GIT in teleosts. Transcriptional analysis of ghrelin splicing variants ( ghrl-1 and ghrl-2 ) showed no difference between treatments. However, correlation analysis showed that the mRNA expression for all genes (restricted to segment with the highest levels) were affected by the residual luminal content. Overall, the results show minimal effects of 4 days of induced fasting in Atlantic salmon, suggesting that more time is needed to initiate a large GIT response., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Del Vecchio, Lai, Gomes, Verri, Kalananthan, Barca, Handeland and Rønnestad.)
- Published
- 2021
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22. Physical and nutrient stimuli differentially modulate gut motility patterns, gut transit rate, and transcriptome in an agastric fish, the ballan wrasse.
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Le HTMD, Lie KK, Etayo A, Rønnestad I, and Sæle Ø
- Subjects
- Animal Nutritional Physiological Phenomena, Animals, Fish Proteins genetics, Fish Proteins metabolism, Fishes metabolism, Intestinal Mucosa metabolism, Fishes physiology, Gastrointestinal Motility, Movement, Nutrients metabolism, Transcriptome
- Abstract
The effects of nutrient and mechanical sensing on gut motility and intestinal metabolism in lower vertebrates remains largely unknown. Here we present the transcriptome response to luminal stimulation by nutrients and an inert bolus on nutrient response pathways and also the response on gut motility in a stomachless fish with a short digestive tract; the ballan wrasse (Labrus berggylta). Using an in vitro model, we differentiate how signals initiated by physical stretch (cellulose and plastic beads) and nutrients (lipid and protein) modulate the gut evacuation rate, motility patterns and the transcriptome. Intestinal stretch generated by inert cellulose initiated a faster evacuation of digesta out of the anterior intestine compared to digestible protein and lipid. Stretch on the intestine upregulated genes associated with increased muscle activity, whereas nutrients stimulated increased expression of several neuropeptides and receptors which are directly involved in gut motility regulation. Although administration of protein and lipid resulted in similar bulbous evacuation times, differences in intestinal motility, transit between the segments and gene expression between the two were observed. Lipid induced increased frequency of ripples and standing contraction in the middle section of the intestine compared to the protein group. We suggest that this difference in motility was modulated by factors [prepronociceptin (pnoca), prodynorphin (pdyn) and neuromedin U (nmu), opioid neurotransmitters and peptides] that are known to inhibit gastrointestinal motility and were upregulated by protein and not lipid. Our findings show that physical pressure in the intestine initiate contractions propelling the bolus distally, directly towards the exit, whereas the stimuli from nutrients modulates the motility to prolong the residence time of digesta in the digestive tract for optimal digestion., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
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23. Assessing microplastic as a vector for chemical entry into fish larvae using a novel tube-feeding approach.
- Author
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Norland S, Vorkamp K, Bogevik AS, Koelmans AA, Diepens NJ, Burgerhout E, Hansen ØJ, Puvanendran V, and Rønnestad I
- Subjects
- Animals, Fishes, Larva, Microplastics, Plastics, Polychlorinated Biphenyls, Water Pollutants, Chemical analysis
- Abstract
A tube-feeding model for administering microplastic (MP, Ø = 30 μm) spheres to fish larvae was employed to quantify the uptake of hydrophobic organic contaminants (HOCs) into the larval body through a single administration of MP. Polychlorinated biphenyl-153 (PCB-153) was used as a representative HOC that can be sorbed to MP in the sea. Atlantic herring (Clupea harengus) larvae (34-51 days post-hatching) were selected as the animal model. The herring larvae were tube-fed a single load of up to 200 polystyrene or polyethylene MP spheres spiked with
14 C-labelled PCB-153, and the control larvae were tube-fed an isotonic solution without MP. At the time of sampling (24 h post feeding), some larvae had evacuated all MP spheres from the gut, while others still had MP remaining in the gut. In larvae with a significant number of MP spheres still present in the gut, whole-body scintillation counting (including the MP in the gut lumen) showed elevated levels of the tracer compared to those in the control fish larvae. For larvae in which all or almost all MP had been evacuated by the time of sampling, the tracer levels of the whole body were not significantly different compared to those for the control fish larvae. These data indicate that there was no significant transfer of PCB-153 from contaminated MP into fish larvae within a gut-transit time of <24 h. This study suggests that the vector role of MP in HOC uptake and absorption may be minor compared to that of other HOC uptake pathways., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)- Published
- 2021
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24. The Melanocortin System in Atlantic Salmon ( Salmo salar L.) and Its Role in Appetite Control.
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Kalananthan T, Lai F, Gomes AS, Murashita K, Handeland S, and Rønnestad I
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The melanocortin system is a key neuroendocrine network involved in the control of food intake and energy homeostasis in vertebrates. Within the hypothalamus, the system comprises two main distinct neuronal cell populations that express the neuropeptides proopiomelanocortin (POMC; anorexigenic) or agouti-related protein (AGRP; orexigenic). Both bind to the melanocortin-4 receptor (MC4R) in higher order neurons that control both food intake and energy expenditure. This system is relatively well-conserved among vertebrates. However, in Atlantic salmon ( Salmo salar L.), the salmonid-specific fourth round whole-genome duplication led to the presence of several paralog genes which might result in divergent functions of the duplicated genes. In the current study, we report the first comprehensive comparative identification and characterization of Mc4r and extend the knowledge of Pomc and Agrp in appetite control in Atlantic salmon. In silico analysis revealed multiple paralogs for mc4r ( a1 , a2 , b1 , and b2 ) in the Atlantic salmon genome and confirmed the paralogs previously described for pomc ( a1 , a2 , and b ) and agrp ( 1 and 2 ). All Mc4r paralogs are relatively well-conserved with the human homolog, sharing at least 63% amino acid sequence identity. We analyzed the mRNA expression of mc4r , pomc , and agrp genes in eight brain regions of Atlantic salmon post-smolt under two feeding states: normally fed and fasted for 4 days. The mc4ra2 and b1 mRNAs were predominantly and equally abundant in the hypothalamus and telencephalon, the mc4rb2 in the hypothalamus, and a1 in the telencephalon. All pomc genes were highly expressed in the pituitary, followed by the hypothalamus and saccus vasculosus. The agrp genes showed a completely different expression pattern from each other, with prevalent expression of the agrp1 in the hypothalamus and agrp2 in the telencephalon. Fasting did not induce any significant changes in the mRNA level of mc4r , agrp , or pomc paralogs in the hypothalamus or in other highly expressed regions between fed and fasted states. The identification and wide distribution of multiple paralogs of mc4r , pomc , and agrp in Atlantic salmon brain provide new insights and give rise to new questions of the melanocortin system in the appetite regulation in Atlantic salmon., (Copyright © 2020 Kalananthan, Lai, Gomes, Murashita, Handeland and Rønnestad.)
- Published
- 2020
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25. Effects of temperature on feeding and digestive processes in fish.
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Volkoff H and Rønnestad I
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As most fish are ectotherms, their physiology is strongly affected by temperature. Temperature affects their metabolic rate and thus their energy balance and behavior, including locomotor and feeding behavior. Temperature influences the ability/desire of the fish to obtain food, and how they process food through digestion, absorb nutrients within the gastrointestinal tract, and store excess energy. As fish display a large variability in habitats, feeding habits, and anatomical and physiological features, the effects of temperature are complex and species-specific. The effects of temperature depend on the timing, intensity, and duration of exposure as well as the speed at which temperature changes occur. Whereas acute short-term variations of temperature might have drastic, often detrimental, effects on fish physiology, long-term gradual variations might lead to acclimation, e.g . variations in metabolic and digestive enzyme profiles. The goal of this review is to summarize our current knowledge on the effects of temperature on energy homeostasis, with specific focus on metabolism, feeding, digestion, and how fish are often able to "adapt" to changing environments through phenotypic and physiological changes., Competing Interests: No potential conflict of interest was reported by the authors., (© 2020 Informa UK Limited, trading as Taylor & Francis Group.)
- Published
- 2020
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26. Hypothalamic agrp and pomc mRNA Responses to Gastrointestinal Fullness and Fasting in Atlantic Salmon ( Salmo salar , L.).
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Kalananthan T, Murashita K, Rønnestad I, Ishigaki M, Takahashi K, Silva MS, Wakabayashi Y, Lai F, Shimizu M, Nilsen TO, Pino Martinez E, and Gomes AS
- Abstract
The orexigenic agouti-related protein (AgRP) and the anorexigenic pro-opiomelanocortin (POMC) are crucial players in the control of feed intake in vertebrates, yet their role in teleosts has not been fully established. Triplicate groups of Atlantic salmon ( Salmo salar ) post smolts were subjected to (1) fasting for 3 days (fast) and (2) normal feeding (fed), resulting in a significant ( p < 0.05) upregulation of hypothalamic agrp1 transcripts levels in the fast group. Moreover, the mRNA abundance of agrp1 was significantly ( p < 0.05) correlated with the stomach dry weight content. Corresponding inverse patterns were observed for pomca2 , albeit not statistically significant. No significant differences were found for the other paralogues, agrp2 and pomca1 and b, between fed and fast groups. The significant correlation between stomach fullness and agrp1 mRNA expression suggests a possible link between the stomach filling/distension and satiety signals. Our study indicates that hypothalamic agrp1 acts as an orexigenic signal in Atlantic salmon., (Copyright © 2020 Kalananthan, Murashita, Rønnestad, Ishigaki, Takahashi, Silva, Wakabayashi, Lai, Shimizu, Nilsen, Pino Martinez and Gomes.)
- Published
- 2020
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27. Correction to: The peptide transporter 1a of the zebrafish Danio rerio , an emerging model in nutrigenomics and nutrition research: molecular characterization, functional properties, and expression analysis.
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Vacca F, Barca A, Gomes AS, Mazzei A, Piccinni B, Cinquetti R, Del Vecchio G, Romano A, Rønnestad I, Bossi E, and Verri T
- Abstract
[This corrects the article DOI: 10.1186/s12263-019-0657-3.]., (© The Author(s) 2020.)
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- 2020
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28. Identification and characterization of the Atlantic salmon peptide transporter 1a.
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Gomes AS, Vacca F, Cinquetti R, Murashita K, Barca A, Bossi E, Rønnestad I, and Verri T
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- Animals, Evolution, Molecular, Fish Proteins chemistry, Fish Proteins genetics, Gene Expression Regulation, Hydrogen-Ion Concentration, Kinetics, Peptide Transporter 1 chemistry, Peptide Transporter 1 genetics, Phylogeny, Salmo salar genetics, Xenopus laevis, Dipeptides metabolism, Fish Proteins metabolism, Intestinal Absorption, Peptide Transporter 1 metabolism, Salmo salar metabolism
- Abstract
Peptide transporter 1 (PepT1) mediates the uptake of dietary di-/tripeptides in vertebrates. However, in teleost fish gut, more than one PepT1-type transporter might operate, because of teleost-specific whole gen(om)e duplication event(s) that occurred during evolution. Here, we describe a novel teleost di-/tripeptide transporter, i.e., the Atlantic salmon ( Salmo salar ) peptide transporter 1a [PepT1a; or solute carrier family 15 member 1a (Slc15a1a)], which is a paralog (77% similarity and 64% identity at the amino acid level) of the well-described Atlantic salmon peptide transporter 1b [PepT1b, alias PepT1; or solute carrier family 15 member 1b (Slc15a1b)]. Comparative analysis and evolutionary relationships of gene/protein sequences were conducted after ad hoc database mining. Tissue mRNA expression analysis was performed by quantitative real-time PCR, whereas transport function analysis was accomplished by heterologous expression in Xenopus laevis oocytes and two-electrode voltage-clamp measurements. Atlantic salmon pept1a is highly expressed in the proximal intestine (pyloric ceca ≈ anterior midgut > midgut >> posterior midgut), in the same gut regions as pept1b but notably ~5-fold less abundant. Like PepT1b, Atlantic salmon PepT1a is a low-affinity/high-capacity system. Functional analysis showed electrogenic, Na
+ -independent/pH-dependent transport and apparent substrate affinity ( K0.5 ) values for Gly-Gln of 1.593 mmol/L at pH 7.6 and 0.076 mmol/L at pH 6.5. In summary, we show that a piscine PepT1a-type transporter is functional. Defining the role of Atlantic salmon PepT1a in the gut will help to understand the evolutionary and functional relationships among peptide transporters. Its functional characterization will contribute to elucidate the relevance of peptide transporters in Atlantic salmon nutritional physiology.- Published
- 2020
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29. The peptide transporter 1a of the zebrafish Danio rerio , an emerging model in nutrigenomics and nutrition research: molecular characterization, functional properties, and expression analysis.
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Vacca F, Barca A, Gomes AS, Mazzei A, Piccinni B, Cinquetti R, Del Vecchio G, Romano A, Rønnestad I, Bossi E, and Verri T
- Abstract
Background: Peptide transporter 1 (PepT1, alias Slc15a1) mediates the uptake of dietary di/tripeptides in all vertebrates. However, in teleost fish, more than one PepT1-type transporter might function, due to specific whole genome duplication event(s) that occurred during their evolution leading to a more complex paralogue gene repertoire than in higher vertebrates (tetrapods)., Results: Here, we describe a novel di/tripeptide transporter in the zebrafish ( Danio rerio ), i.e., the zebrafish peptide transporter 1a (PepT1a; also known as Solute carrier family 15 member a1, Slc15a1a), which is a paralogue (78% similarity, 62% identity at the amino acid level) of the previously described zebrafish peptide transporter 1b (PepT1b, alias PepT1; also known as Solute carrier family 15 member 1b, Slc15a1b). Also, we report a basic analysis of the pept1a ( slc15a1a ) mRNA expression levels in zebrafish adult tissues/organs and embryonic/early larval developmental stages. As assessed by expression in Xenopus laevis oocytes and two-electrode voltage clamp measurements, zebrafish PepT1a, as PepT1b, is electrogenic, Na
+ -independent, and pH-dependent and functions as a low-affinity system, with K0.5 values for Gly-Gln at - 60 mV of 6.92 mmol/L at pH 7.6 and 0.24 mmol/L at pH 6.5 and at - 120 mV of 3.61 mmol/L at pH 7.6 and 0.45 mmol/L at pH 6.5. Zebrafish pept1a mRNA is highly expressed in the intestine and ovary of the adult fish, while its expression in early development undergoes a complex trend over time, with pept1a mRNA being detected 1 and 2 days post-fertilization (dpf), possibly due to its occurrence in the RNA maternal pool, decreasing at 3 dpf (~ 0.5-fold) and increasing above the 1-2 dpf levels at 4 to 7 dpf, with a peak (~ 7-fold) at 6 dpf., Conclusions: We show that the zebrafish PepT1a-type transporter is functional and co-expressed with pept1b ( slc15a1b ) in the adult fish intestine. Its expression is also confirmed during the early phases of development when the yolk syncytial layer is present and yolk protein resorption processes are active. While completing the missing information on PepT1-type transporters function in the zebrafish, these results open to future investigations on the similar/differential role(s) of PepT1a/PepT1b in zebrafish and teleost fish physiology., Competing Interests: Competing interestsThe authors declare that they have no competing interests., (© The Author(s) 2019.)- Published
- 2019
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30. Effects of Cholecystokinin (CCK) on Gut Motility in the Stomachless Fish Ballan Wrasse ( Labrus bergylta ).
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Le HTMD, Lie KK, Giroud-Argoud J, Rønnestad I, and Sæle Ø
- Abstract
Cholecystokinin (CCK) is well-known as a key hormone that inhibits stomach emptying and stimulates midgut motility in gastric species. However, the function of CCK related to gut motility in agastric fish, especially in fish with a short digestive tract such as ballan wrasse, remains unknown. Here we present a detailed description of the spatio-temporal quantification of intestinal motility activity in vitro comprising the complete intestinal tract in ballan wrasse. We show that CCK modulates intestinal motility, having multiple effects on motility patterns depending on location in the gut and types of contractions. CCK reduced propagating contractions in the foregut, but it increased both non-propagating and propagating contractions in the hindgut. CCK also altered the direction of propagating contractions, as it reduced anterograde ripples and slow propagating contractions. The velocity of propagating contractions was slowed down by CCK. CCK also reduced the amplitude of standing contractions and ripples, but it did not alter the amplitude of slow propagating contractions. The presence of CCKA receptor antagonist modulated the motility responses of ballan wrasse intestines when exposed to CCK. We also showed that CCK reduced the intestinal length and stimulated motility to empty the gallbladder. Based on our findings we hypothesize that CCK, mainly through the CCKA receptor, modulates non-propagating and propagating contractions to optimize digestion and absorption and regulate the intestinal evacuation in ballan wrasse. We also found evidence that the modulation of intestinal motility by CCK is different in agastric fish from that in gastric vertebrates. We suggest that this is an evolutionary adaptation to optimize digestion without a stomach.
- Published
- 2019
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31. Loss of stomach, loss of appetite? Sequencing of the ballan wrasse (Labrus bergylta) genome and intestinal transcriptomic profiling illuminate the evolution of loss of stomach function in fish.
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Lie KK, Tørresen OK, Solbakken MH, Rønnestad I, Tooming-Klunderud A, Nederbragt AJ, Jentoft S, and Sæle Ø
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- Animals, Appetite, Digestion, Gastrointestinal Tract, Genome, Perciformes physiology, Phylogeny, Biological Evolution, Gene Expression Profiling, Perciformes genetics, Stomach physiology
- Abstract
Background: The ballan wrasse (Labrus bergylta) belongs to a large teleost family containing more than 600 species showing several unique evolutionary traits such as lack of stomach and hermaphroditism. Agastric fish are found throughout the teleost phylogeny, in quite diverse and unrelated lineages, indicating stomach loss has occurred independently multiple times in the course of evolution. By assembling the ballan wrasse genome and transcriptome we aimed to determine the genetic basis for its digestive system function and appetite regulation. Among other, this knowledge will aid the formulation of aquaculture diets that meet the nutritional needs of agastric species., Results: Long and short read sequencing technologies were combined to generate a ballan wrasse genome of 805 Mbp. Analysis of the genome and transcriptome assemblies confirmed the absence of genes that code for proteins involved in gastric function. The gene coding for the appetite stimulating protein ghrelin was also absent in wrasse. Gene synteny mapping identified several appetite-controlling genes and their paralogs previously undescribed in fish. Transcriptome profiling along the length of the intestine found a declining expression gradient from the anterior to the posterior, and a distinct expression profile in the hind gut., Conclusions: We showed gene loss has occurred for all known genes related to stomach function in the ballan wrasse, while the remaining functions of the digestive tract appear intact. The results also show appetite control in ballan wrasse has undergone substantial changes. The loss of ghrelin suggests that other genes, such as motilin, may play a ghrelin like role. The wrasse genome offers novel insight in to the evolutionary traits of this large family. As the stomach plays a major role in protein digestion, the lack of genes related to stomach digestion in wrasse suggests it requires formulated diets with higher levels of readily digestible protein than those for gastric species.
- Published
- 2018
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32. Appetite-Controlling Endocrine Systems in Teleosts.
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Rønnestad I, Gomes AS, Murashita K, Angotzi R, Jönsson E, and Volkoff H
- Abstract
Mammalian studies have shaped our understanding of the endocrine control of appetite and body weight in vertebrates and provided the basic vertebrate model that involves central (brain) and peripheral signaling pathways as well as environmental cues. The hypothalamus has a crucial function in the control of food intake, but other parts of the brain are also involved. The description of a range of key neuropeptides and hormones as well as more details of their specific roles in appetite control continues to be in progress. Endocrine signals are based on hormones that can be divided into two groups: those that induce (orexigenic), and those that inhibit (anorexigenic) appetite and food consumption. Peripheral signals originate in the gastrointestinal tract, liver, adipose tissue, and other tissues and reach the hypothalamus through both endocrine and neuroendocrine actions. While many mammalian-like endocrine appetite-controlling networks and mechanisms have been described for some key model teleosts, mainly zebrafish and goldfish, very little knowledge exists on these systems in fishes as a group. Fishes represent over 30,000 species, and there is a large variability in their ecological niches and habitats as well as life history adaptations, transitions between life stages and feeding behaviors. In the context of food intake and appetite control, common adaptations to extended periods of starvation or periods of abundant food availability are of particular interest. This review summarizes the recent findings on endocrine appetite-controlling systems in fish, highlights their impact on growth and survival, and discusses the perspectives in this research field to shed light on the intriguing adaptations that exist in fish and their underlying mechanisms.
- Published
- 2017
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33. The two-step development of a duplex retina involves distinct events of cone and rod neurogenesis and differentiation.
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Valen R, Eilertsen M, Edvardsen RB, Furmanek T, Rønnestad I, van der Meeren T, Karlsen Ø, Nilsen TO, and Helvik JV
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- Animals, Eye Proteins biosynthesis, Eye Proteins genetics, Gadus morhua embryology, Gadus morhua genetics, Gene Duplication, Larva, Life Cycle Stages, Metamorphosis, Biological, Opsins genetics, Receptors, Cytoplasmic and Nuclear biosynthesis, Receptors, Cytoplasmic and Nuclear genetics, Retina cytology, Retina embryology, Transcription Factors biosynthesis, Transcription Factors genetics, Transcriptome, Vision, Ocular, Gadus morhua growth & development, Gene Expression Regulation, Developmental, Neurogenesis, Retina growth & development, Retinal Rod Photoreceptor Cells cytology
- Abstract
Unlike in mammals, persistent postembryonic retinal growth is a characteristic feature of fish, which includes major remodeling events that affect all cell types including photoreceptors. Consequently, visual capabilities change during development, where retinal sensitivity to different wavelengths of light (photopic vision), -and to limited photons (scotopic vision) are central capabilities for survival. Differently from well-established model fish, Atlantic cod has a prolonged larval stage where only cone photoreceptors are present. Rods do not appear until juvenile transition (metamorphosis), a hallmark of indirect developing species. Previously we showed that whole gene families of lws (red-sensitive) and sws1 (UV-sensitive) opsins have been lost in cod, while rh2a (green-sensitive) and sws2 (blue-sensitive) genes have tandem duplicated. Here, we provide a comprehensive characterization of a two-step developing duplex retina in Atlantic cod. The study focuses on cone subtype dynamics and delayed rod neurogenesis and differentiation in all cod life stages. Using transcriptomic and histological approaches we show that different opsins disappear in a topographic manner during development where central to peripheral retina is a key axis of expressional change. Early cone differentiation was initiated in dorso-temporal retina different from previously described in fish. Rods first appeared during initiation of metamorphosis and expression of the nuclear receptor transcription factor nr2e3-1, suggest involvement in rod specification. The indirect developmental strategy thus allows for separate studies of cones and rods development, which in nature correlates with visual changes linked to habitat shifts. The clustering of key retinal genes according to life stage, suggests that Atlantic cod with its sequenced genome may be an important resource for identification of underlying factors required for development and function of photopic and scotopic vision., (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2016
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34. First feed affects the expressions of microRNA and their targets in Atlantic cod - CORRIGENDUM.
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Bizuayehu TT, Furmanek T, Karlsen Ø, van der Meeren T, Edvardsen RB, Rønnestad I, Hamre K, Johansen SD, and Babiak I
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- 2016
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35. The transcriptome of metamorphosing flatfish.
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Alves RN, Gomes AS, Stueber K, Tine M, Thorne MA, Smáradóttir H, Reinhard R, Clark MS, Rønnestad I, and Power DM
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- Animals, Cluster Analysis, Computational Biology methods, Gene Expression Profiling, Gene Expression Regulation, Developmental drug effects, Gene Ontology, High-Throughput Nucleotide Sequencing, Molecular Sequence Annotation, Morphogenesis genetics, Organ Specificity, Thyroid Hormones pharmacology, Flatfishes genetics, Metamorphosis, Biological genetics, Transcriptome
- Abstract
Background: Flatfish metamorphosis denotes the extraordinary transformation of a symmetric pelagic larva into an asymmetric benthic juvenile. Metamorphosis in vertebrates is driven by thyroid hormones (THs), but how they orchestrate the cellular, morphological and functional modifications associated with maturation to juvenile/adult states in flatfish is an enigma. Since THs act via thyroid receptors that are ligand activated transcription factors, we hypothesized that the maturation of tissues during metamorphosis should be preceded by significant modifications in the transcriptome. Targeting the unique metamorphosis of flatfish and taking advantage of the large size of Atlantic halibut (Hippoglossus hippoglossus) larvae, we determined the molecular basis of TH action using RNA sequencing., Results: De novo assembly of sequences for larval head, skin and gastrointestinal tract (GI-tract) yielded 90,676, 65,530 and 38,426 contigs, respectively. More than 57 % of the assembled sequences were successfully annotated using a multi-step Blast approach. A unique set of biological processes and candidate genes were identified specifically associated with changes in morphology and function of the head, skin and GI-tract. Transcriptome dynamics during metamorphosis were mapped with SOLiD sequencing of whole larvae and revealed greater than 8,000 differentially expressed (DE) genes significantly (p < 0.05) up- or down-regulated in comparison with the juvenile stage. Candidate transcripts quantified by SOLiD and qPCR analysis were significantly (r = 0.843; p < 0.05) correlated. The majority (98 %) of DE genes during metamorphosis were not TH-responsive. TH-responsive transcripts clustered into 6 groups based on their expression pattern during metamorphosis and the majority of the 145 DE TH-responsive genes were down-regulated., Conclusions: A transcriptome resource has been generated for metamorphosing Atlantic halibut and over 8,000 DE transcripts per stage were identified. Unique sets of biological processes and candidate genes were associated with changes in the head, skin and GI-tract during metamorphosis. A small proportion of DE transcripts were TH-responsive, suggesting that they trigger gene networks, signalling cascades and transcription factors, leading to the overt changes in tissue occurring during metamorphosis.
- Published
- 2016
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36. The Ontogeny and Brain Distribution Dynamics of the Appetite Regulators NPY, CART and pOX in Larval Atlantic Cod (Gadus morhua L.).
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Le HT, Angotzi AR, Ebbesson LO, Karlsen Ø, and Rønnestad I
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- Animals, Brain cytology, Fish Proteins genetics, Gene Expression Regulation, Developmental, Gene Ontology, In Situ Hybridization, Fluorescence, Larva cytology, Nerve Tissue Proteins genetics, Neuropeptides genetics, Appetite Regulation physiology, Brain metabolism, Fish Proteins metabolism, Gadus morhua physiology, Larva physiology, Nerve Tissue Proteins metabolism, Neuropeptides metabolism
- Abstract
Similar to many marine teleost species, Atlantic cod undergo remarkable physiological changes during the early life stages with concurrent and profound changes in feeding biology and ecology. In contrast to the digestive system, very little is known about the ontogeny and the localization of the centers that control appetite and feed ingestion in the developing brain of fish. We examined the expression patterns of three appetite regulating factors (orexigenic: neuropeptide Y, NPY; prepro-orexin, pOX and anorexigenic: cocaine- and amphetamine-regulated transcript, CART) in discrete brain regions of developing Atlantic cod using chromogenic and double fluorescent in situ hybridization. Differential temporal and spatial expression patterns for each appetite regulator were found from first feeding (4 days post hatch; dph) to juvenile stage (76 dph). Neurons expressing NPY mRNA were detected in the telencephalon (highest expression), diencephalon, and optic tectum from 4 dph onward. CART mRNA expression had a wider distribution along the anterior-posterior brain axis, including both telencephalon and diencephalon from 4 dph. From 46 dph, CART transcripts were also detected in the olfactory bulb, region of the nucleus of medial longitudinal fascicle, optic tectum and midbrain tegmentum. At 4 and 20 dph, pOX mRNA expression was exclusively found in the preoptic region, but extended to the hypothalamus at 46 and 76 dph. Co-expression of both CART and pOX genes were also observed in several hypothalamic neurons throughout larval development. Our results show that both orexigenic and anorexigenic factors are present in the telencephalon, diencephalon and mesencephalon in cod larvae. The telencephalon mostly contains key factors of hunger control (NPY), while the diencephalon, and particularly the hypothalamus may have a more complex role in modulating the multifunctional control of appetite in this species. As the larvae develop, the overall progression in temporal and spatial complexity of NPY, CART and pOX mRNAs expression might be correlated to the maturation of appetite control regulation. These observations suggest that teleost larvae continue to develop the regulatory networks underlying appetite control after onset of exogenous feeding.
- Published
- 2016
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37. 1H NMR metabolic profiling of cod (Gadus morhua) larvae: potential effects of temperature and diet composition during early developmental stages.
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Chauton MS, Galloway TF, Kjørsvik E, Størseth TR, Puvanendran V, van der Meeren T, Karlsen Ø, Rønnestad I, and Hamre K
- Abstract
Marine aquaculture offers a great source of protein for the increasing human population, and farming of, for example, Atlantic salmon is a global industry. Atlantic cod farming however, is an example of a promising industry where the potential is not yet realized. Research has revealed that a major bottleneck to successful farming of cod is poor quality of the larvae and juveniles. A large research program was designed to increase our understanding of how environmental factors such as temperature and nutrition affects cod larvae development. Data on larvae growth and development were used together with nuclear magnetic resonance. The NMR data indicated that the temperature influenced the metabolome of the larvae; differences were related to osmolytes such as betaine/TMAO, the amino acid taurine, and creatine and lactate which reflect muscle activity. The larvae were fed Artemia from stage 2, and this was probably reflected in a high taurine content of older larvae. Larvae fed with copepods in the nutrition experiment also displayed a high taurine content, together with higher creatine and betaine/TMAO content. Data on the cod larvae metabolome should be coupled to data on gene expression, in order to identify events which are regulated on the genetic level versus regulation resulting from temperature or nutrition during development, to fully understand how the environment affects larval development., (© 2015. Published by The Company of Biologists Ltd.)
- Published
- 2015
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38. Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae.
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Penglase S, Edvardsen RB, Furmanek T, Rønnestad I, Karlsen Ø, van der Meeren T, and Hamre K
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- Animals, Gadus morhua growth & development, Gene Expression Regulation, Glutathione metabolism, High-Throughput Nucleotide Sequencing, NF-E2-Related Factor 2 genetics, NF-E2-Related Factor 2 metabolism, Oxidation-Reduction, Oxidative Stress, RNA chemistry, RNA metabolism, Sequence Analysis, RNA, Diet, Gadus morhua metabolism
- Abstract
The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear. In the following study we compared the effects of such diets on redox regulation pathways during development of Atlantic cod (Gadus morhua) larvae. Cod larvae were fed a control diet of copepods or the typical rotifer/Artemia diet commonly used in commercial marine fish hatcheries, from first feeding until after metamorphosis. The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development. We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae. This rotifer/Artemia diet-induced differential regulation of the redox system was greatest during periods of suboptimal growth. Upregulation of the oxidative stress response transcription factor, nrf2, and NRF2 target genes in rotifer/Artemia fed larvae suggest this diet induced an NRF2-mediated oxidative stress response. Overall, the data demonstrate that nutritional intake plays a role in regulating the redox system in developing fish larvae. This may be a factor in dietary-induced differences observed in larval growth., (Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2015
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39. Copepods enhance nutritional status, growth and development in Atlantic cod (Gadus morhua L.) larvae - can we identify the underlying factors?
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Karlsen Ø, van der Meeren T, Rønnestad I, Mangor-Jensen A, Galloway TF, Kjørsvik E, and Hamre K
- Abstract
The current commercial production protocols for Atlantic cod depend on enriched rotifers and Artemia during first-feeding, but development and growth remain inferior to fish fed natural zooplankton. Two experiments were conducted in order to identify the underlying factors for this phenomenon. In the first experiment (Exp-1), groups of cod larvae were fed either (a) natural zooplankton, mainly copepods, increasing the size of prey as the larvae grew or (b) enriched rotifers followed by Artemia (the intensive group). In the second experiment (Exp-2), two groups of larvae were fed as in Exp-1, while a third group was fed copepod nauplii (approximately the size of rotifers) throughout the larval stage. In both experiments, growth was not significantly different between the groups during the first three weeks after hatching, but from the last part of the rotifer feeding period and onwards, the growth of the larvae fed copepods was higher than that of the intensive group. In Exp-2, the growth was similar between the two copepod groups during the expeimental period, indicating that nutrient composition, not prey size caused the better growth on copepods. Analyses of the prey showed that total fatty acid composition and the ratio of phospholipids to total lipids was slightly different in the prey organisms, and that protein, taurine, astaxanthin and zinc were lower on a dry weight basis in rotifers than in copepods. Other measured nutrients as DHA, all analysed vitamins, manganese, copper and selenium were similar or higher in the rotifers. When compared to the present knowledge on nutrient requirements, protein and taurine appeared to be the most likely limiting nutrients for growth in cod larvae fed rotifers and Artemia. Larvae fed rotifers/Artemia had a higher whole body lipid content than larvae fed copepods at the end of the experiment (stage 5) after the fish had been fed the same formulated diet for approximately 2 weeks.
- Published
- 2015
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40. Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon.
- Author
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Espe M, Andersen SM, Holen E, Rønnestad I, Veiseth-Kent E, Zerrahn JE, and Aksnes A
- Subjects
- Acetyltransferases genetics, Acetyltransferases metabolism, Adenosylmethionine Decarboxylase genetics, Adenosylmethionine Decarboxylase metabolism, Animals, Aquaculture, Deficiency Diseases metabolism, Deficiency Diseases prevention & control, Diet adverse effects, Energy Intake, Fish Proteins genetics, Fish Proteins metabolism, Gene Expression Regulation, Developmental, Lipid Metabolism, Liver growth & development, Liver pathology, Methionine metabolism, Methionine therapeutic use, Muscle, Skeletal metabolism, Muscle, Skeletal pathology, Norway, Ornithine Decarboxylase genetics, Ornithine Decarboxylase metabolism, Plant Proteins adverse effects, Putrescine metabolism, Salmo salar metabolism, Spermine metabolism, Weight Gain, Deficiency Diseases veterinary, Diet veterinary, Liver metabolism, Methionine deficiency, Polyamines metabolism, S-Adenosylmethionine metabolism, Salmo salar growth & development
- Abstract
During the last few decades, plant protein ingredients such as soya proteins have replaced fishmeal in the diets of aquacultured species. This may affect the requirement and metabolism of methionine as soya contains less methionine compared with fishmeal. To assess whether methionine limitation affects decarboxylated S-adenosylmethionine availability and polyamine status, in the present study, juvenile Atlantic salmon were fed a methionine-deficient plant protein-based diet or the same diet supplemented with dl-methionine for 8 weeks. The test diets were compared with a fishmeal-based control diet to assess their effects on the growth performance of fish. Methionine limitation reduced growth and protein accretion, but when fish were fed the dl-methionine-supplemented diet their growth and protein accretion equalled those of fish fed the fishmeal-based control diet. Methionine limitation reduced free methionine concentrations in the plasma and muscle, while those in the liver were not affected. S-adenosylmethionine (SAM) concentrations were higher in the liver of fish fed the methionine-deficient diet, while S-adenosylhomocysteine concentrations were not affected. Putrescine concentrations were higher and spermine concentrations were lower in the liver of fish fed the methionine-deficient diet, while the gene expression of SAM decarboxylase (SAMdc) and the rate-limiting enzyme of polyamine synthesis ornithine decarboxylase (ODC) was not affected. Polyamine turnover, as assessed by spermine/spermidine acetyltransferase (SSAT) abundance, activity and gene expression, was not affected by treatment. However, the gene expression of the cytokine TNF-α increased in fish fed the methionine-deficient diet, indicative of stressful conditions in the liver. Even though taurine concentrations in the liver were not affected by treatment, methionine and taurine concentrations in muscle decreased due to methionine deficiency. Concomitantly, liver phospholipid and cholesterol concentrations were reduced, while NEFA concentrations were elevated. In conclusion, methionine deficiency did not increase polyamine turnover through depletion of hepatic SAM, as assessed by SSAT activity and abundance.
- Published
- 2014
- Full Text
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41. Functional modifications associated with gastrointestinal tract organogenesis during metamorphosis in Atlantic halibut (Hippoglossus hippoglossus).
- Author
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Gomes AS, Kamisaka Y, Harboe T, Power DM, and Rønnestad I
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Fish Proteins genetics, Flounder growth & development, Flounder physiology, Gastric Acid metabolism, Gastrointestinal Motility physiology, Gastrointestinal Tract growth & development, Gastrointestinal Tract physiology, Gene Expression Regulation, Developmental, Ghrelin genetics, Hydrogen-Ion Concentration, Larva genetics, Larva growth & development, Larva physiology, Molecular Sequence Data, Muscle Contraction physiology, Organ Size, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Sodium-Potassium-Exchanging ATPase classification, Sodium-Potassium-Exchanging ATPase genetics, Time Factors, Vertebrates classification, Vertebrates genetics, Flounder genetics, Gastrointestinal Tract metabolism, Metamorphosis, Biological genetics, Organogenesis genetics
- Abstract
Background: Flatfish metamorphosis is a hormone regulated post-embryonic developmental event that transforms a symmetric larva into an asymmetric juvenile. In altricial-gastric teleost fish, differentiation of the stomach takes place after the onset of first feeding, and during metamorphosis dramatic molecular and morphological modifications of the gastrointestinal (GI-) tract occur. Here we present the functional ontogeny of the developing GI-tract from an integrative perspective in the pleuronectiforme Atlantic halibut, and test the hypothesis that the multiple functions of the teleost stomach develop synchronously during metamorphosis., Results: Onset of gastric function was determined with several approaches (anatomical, biochemical, molecular and in vivo observations). In vivo pH analysis in the GI-tract lumen combined with quantitative PCR (qPCR) of α and β subunits of the gastric proton pump (H+/K+-ATPase) and pepsinogen A2 indicated that gastric proteolytic capacity is established during the climax of metamorphosis. Transcript abundance of ghrelin, a putative orexigenic signalling molecule produced in the developing stomach, correlated (p < 0.05) with the emergence of gastric proteolytic activity, suggesting that the stomach's role in appetite regulation occurs simultaneously with the establishment of proteolytic function. A 3D models series of the GI-tract development indicated a functional pyloric sphincter prior to first feeding. Observations of fed larvae in vivo confirmed that stomach reservoir function was established before metamorphosis, and was thus independent of this event. Mechanical breakdown of food and transportation of chyme through the GI-tract was observed in vivo and resulted from phasic and propagating contractions established well before metamorphosis. The number of contractions in the midgut decreased at metamorphic climax synchronously with establishment of the stomach's proteolytic capacity and its increased peristaltic activity. Putative osmoregulatory competence of the GI-tract, inferred by abundance of Na+/K+-ATPase α transcripts, was already established at the onset of exogenous feeding and was unmodified by metamorphosis., Conclusions: The functional specialization of the GI-tract was not exclusive to metamorphosis, and its osmoregulatory capacity and reservoir function were established before first feeding. Nonetheless, acid production and the proteolytic capacity of the stomach coincided with metamorphic climax, and also marked the onset of the stomach's involvement in appetite regulation via ghrelin.
- Published
- 2014
- Full Text
- View/download PDF
42. Dietary arginine affects energy metabolism through polyamine turnover in juvenile Atlantic salmon (Salmo salar).
- Author
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Andersen SM, Holen E, Aksnes A, Rønnestad I, Zerrahn JE, and Espe M
- Subjects
- Acetyltransferases biosynthesis, Acetyltransferases genetics, Acetyltransferases metabolism, Adipose Tissue, White enzymology, Adipose Tissue, White growth & development, Adipose Tissue, White metabolism, Animals, Aquaculture, Arginine administration & dosage, Carnitine O-Palmitoyltransferase biosynthesis, Carnitine O-Palmitoyltransferase genetics, Carnitine O-Palmitoyltransferase metabolism, Diet adverse effects, Dietary Proteins adverse effects, Dietary Proteins metabolism, Enzyme Induction, Fish Proteins biosynthesis, Fish Proteins genetics, Fish Proteins metabolism, Isoenzymes biosynthesis, Isoenzymes genetics, Isoenzymes metabolism, Lipid Metabolism, Liver enzymology, Liver growth & development, Liver metabolism, Muscle, Skeletal growth & development, Muscle, Skeletal metabolism, Ornithine blood, Ornithine metabolism, Ornithine Decarboxylase biosynthesis, Ornithine Decarboxylase genetics, Ornithine Decarboxylase metabolism, Plant Proteins adverse effects, Plant Proteins metabolism, Putrescine metabolism, Salmo salar blood, Salmo salar growth & development, Arginine metabolism, Diet veterinary, Dietary Supplements, Energy Metabolism, Polyamines metabolism, Salmo salar metabolism
- Abstract
In the present study, quadruplicate groups of juvenile Atlantic salmon (Salmo salar) were fed plant protein-based diets with increasing arginine inclusions (range 28·8-37·4 g/kg DM) to investigate whether arginine supplementation affects growth and lipid accumulation through an elevated polyamine turnover. Dietary lysine was held at a constant concentration, just below the requirement. All other amino acids were balanced and equal in the diets. Arginine supplementation increased protein and fat accretion, without affecting the hepatosomatic or visceralsomatic indices. Dietary arginine correlated with putrescine in the liver (R 0·78, P= 0·01) and with ornithine in the muscle, liver and plasma (P= 0·0002, 0·003 and 0·0002, respectively). The mRNA of ornithine decarboxylase, the enzyme producing putrescine, was up-regulated in the white adipose tissue of fish fed the high-arginine inclusion compared with those fed the low-arginine diet. Concomitantly, spermidine/spermine-(N1)-acetyltransferase, the rate-limiting enzyme for polyamine turnover that consumes acetyl-CoA, showed an increased activity in the liver of fish fed the arginine-supplemented diets. In addition, lower acetyl-CoA concentrations were observed in the liver of fish fed the high-arginine diet, while ATP, which is used in the process of synthesising spermidine and spermine, did not show a similar trend. Gene expression of the rate-limiting enzyme for β-oxidation of long-chain fatty acids, carnitine palmitoyl transferase-1, was up-regulated in the liver of fish fed the high-arginine diet. Taken together, the data support that increased dietary arginine activates polyamine turnover and β-oxidation in the liver of juvenile Atlantic salmon and may act to improve the metabolic status of the fish.
- Published
- 2013
- Full Text
- View/download PDF
43. Cholecystokinin receptors in Atlantic salmon: molecular cloning, gene expression, and structural basis.
- Author
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Rathore RM, Angotzi AR, Jordal AE, and Rønnestad I
- Abstract
The peptide hormone cholecystokinin (CCK) exerts a wide range of digestive and CNS-related physiological signaling via CCK receptors in brain and gut. There is very limited information available on these receptors in Atlantic salmon. The aim of this study was to characterize CCK receptors in gut and brain of salmon. We have identified and cloned one CCK-1 receptor and duplicates of CCK-2 receptor in salmon. The phylogenetic analysis indicates the existence of one common ancestor gene for all CCK receptors. CCK-1R mRNA is highly expressed in pancreas followed by midgut, hindgut, gallbladder, and stomach indicating an involvement in pancreatic regulation and gallbladder contractions. CCK-2R1/gastrin mRNA is expressed at high levels in midgut and at relatively low levels in stomach, gallbladder, and pancreas. We postulate CCK-2R1/gastrin receptor to have gastrin-related functions because of its distribution and abundance in gastro-intestinal (GI) tissues. CCK-2R2 is relatively abundant in brain but has low expression levels in gut tissues supporting the hypothesis for involvement in the gut-brain signaling. Major functional motifs and ligand interaction sites in salmon are conserved with that of mammals. This information will be instrumental for comparative studies and further targeting receptor activation and selectivity of biological responses of CCK in salmon.
- Published
- 2013
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44. Molecular cloning and functional expression of atlantic salmon peptide transporter 1 in Xenopus oocytes reveals efficient intestinal uptake of lysine-containing and other bioactive di- and tripeptides in teleost fish.
- Author
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Rønnestad I, Murashita K, Kottra G, Jordal AE, Narawane S, Jolly C, Daniel H, and Verri T
- Subjects
- Animals, Base Sequence, Biological Transport genetics, Cloning, Molecular, DNA, Complementary analysis, Digestion, Hydrogen-Ion Concentration, Intestinal Absorption, Molecular Sequence Data, Oocytes metabolism, Peptide Transporter 1, RNA, Messenger metabolism, Salmo salar genetics, Symporters metabolism, Tissue Distribution, Xenopus genetics, Xenopus metabolism, Dietary Proteins pharmacokinetics, Gene Expression, Lysine pharmacokinetics, Oligopeptides pharmacokinetics, Salmo salar metabolism, Symporters genetics
- Abstract
Atlantic salmon (Salmo salar L.) is one of the most economically important cultured fish and also a key model species in fish nutrition. During digestion, dietary proteins are enzymatically cleaved and a fraction of degradation products in the form of di- and tripeptides translocates from the intestinal lumen into the enterocyte via the Peptide Transporter 1 (PepT1). With this in mind, a full-length cDNA encoding the Atlantic salmon PepT1 (asPepT1) was cloned and functionally characterized. When overexpressed in Xenopus laevis oocytes, asPepT1 operated as a low-affinity/high-capacity transport system, and its maximal transport activity slightly increased as external proton concentration decreased (varying extracellular pH from 6.5 to 8.5). A total of 19 tested di- and tripeptides, some with acknowledged bioactive properties, some containing lysine, which is conditionally growth limiting in fish, were identified as well transported substrates, with affinities ranging between approximately 0.5 and approximately 1.5 mmol/L. Analysis of body tissue distribution showed the highest levels of asPepT1 mRNA in the digestive tract. In particular, asPepT1 mRNA was present in all segments after the stomach, with higher levels in the pyloric caeca and midgut region and lower levels in the hindgut. Depriving salmon of food for 6 d resulted in a approximately 70% reduction of intestinal PepT1 mRNA levels. asPepT1 will allow systematic in vitro analysis of transport of selected di- and tripeptides that may be generated in Atlantic salmon intestine during gastrointestinal transit. Also, asPepT1 will be useful as a marker to estimate protein absorption function along the intestine under various physiological and pathological conditions.
- Published
- 2010
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45. Whether depositing fat or losing weight, fish maintain a balance.
- Author
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Brix O, Grüner R, Rønnestad I, and Gemballa S
- Subjects
- Animals, Adipose Tissue physiology, Energy Metabolism physiology, Fishes physiology, Weight Loss physiology
- Abstract
In fish, the relative amount of tissues of different densities changes significantly over short periods throughout the year, depending on the availability of food, nutrition and their developmental status, such as sexual maturation. If a land-living animal accumulates fat it influences not only its general state of health, but also markedly increases its energy expenditure for locomotion owing to the force of gravity. On a body submerged in water, this force, which acts on the centre of gravity (COG), is counterbalanced by a lifting force that is negligible in air and which acts on the centre of buoyancy (COB). Any difference in the longitudinal positions of the two centres will therefore result in pitching moments that must be counteracted by body or fin movements. The displacement of the COG away from the COB is a result of tissues of different density (e.g. bones and fat) not being distributed homogeneously along the body axis. Moreover, the proportions of tissues of different densities change significantly with feeding status. It is still unknown whether these changes produce a displacement of the COG and thus affect the hydrostatic stability of fish. Analysis of computed tomography and magnetic resonance imaging images of Atlantic herring, Atlantic salmon and Atlantic mackerel reveals that the COG is fairly constant in each species, although we recorded major interspecies differences in the relative amount of fat, muscle and bone. We conclude that the distribution of different tissues along the body axis is very closely adjusted to the swimming mode of the fish by keeping the COG constant, independent of the body fat status, and that fish can cope with large variations in energy intake without jeopardizing their COG and thus their swimming performance.
- Published
- 2009
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46. Oligopeptide transporter PepT1 in Atlantic cod (Gadus morhua L.): cloning, tissue expression and comparative aspects.
- Author
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Rønnestad I, Gavaia PJ, Viegas CS, Verri T, Romano A, Nilsen TO, Jordal AE, Kamisaka Y, and Cancela ML
- Subjects
- Amino Acid Sequence, Amino Acid Substitution, Animals, Base Sequence, Cloning, Molecular, Digestive System metabolism, Gene Expression Regulation, Humans, Molecular Sequence Data, Peptide Transporter 1, Phylogeny, RNA, Messenger genetics, RNA, Messenger metabolism, Sequence Alignment, Sequence Analysis, DNA, Symporters chemistry, Tissue Distribution, Zebrafish Proteins, Gadus morhua genetics, Oligopeptides metabolism, Symporters genetics, Symporters metabolism
- Abstract
A novel full-length cDNA that encodes for the Atlantic cod (Gadus morhua L.) PepT1-type oligopeptide transporter has been cloned. This cDNA (named codPepT1) was 2,838 bp long, with an open reading frame of 2,190 bp encoding a putative protein of 729 amino acids. Comparison of the predicted Atlantic cod PepT1 protein with zebrafish, bird and mammalian orthologs allowed detection of many structural features that are highly conserved among all the vertebrate proteins analysed, including (1) a larger than expected area of hydrophobic amino acids in close proximity to the N terminus; (2) a single highly conserved cAMP/cGMP-dependent protein kinase phosphorylation motif; (3) a large N-glycosylation-rich region within the large extracellular loop; and (4) a conserved and previously undescribed stretch of 8-12 amino acid residues within the large extracellular loop. Expression analysis at the mRNA level indicated that Atlantic cod PepT1 is mainly expressed at intestinal level, but that it is also present in kidney and spleen. Analysis of its regional distribution along the intestinal tract of the fish revealed that PepT1 is ubiquitously expressed in all segments beyond the stomach, including the pyloric caeca, and through the whole midgut. Only in the last segment, which included the hindgut, was there a lower expression. Atlantic cod PepT1, the second teleost fish PepT1-type transporter documented to date, will contribute to the elucidation of the evolutionary and functional relationships among vertebrate peptide transporters. Moreover, it can represent a useful tool for the study of gut functional regionalization, as well as a marker for the analysis of temporal and spatial expression during ontogeny.
- Published
- 2007
- Full Text
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47. Dietary protein:lipid ratio and lipid nature affects fatty acid absorption and metabolism in a teleost larva.
- Author
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Morais S, Koven W, Rønnestad I, Dinis MT, and Conceição LE
- Subjects
- Absorption, Animals, Artemia metabolism, Docosahexaenoic Acids metabolism, Fatty Acids, Nonesterified metabolism, Flatfishes growth & development, Lipid Peroxides metabolism, Liver metabolism, Oleic Acid metabolism, Phosphatidylcholines metabolism, Stearic Acids metabolism, Triolein metabolism, Dietary Proteins metabolism, Fatty Acids metabolism, Flatfishes metabolism, Lipid Metabolism
- Abstract
Studies with teleost larvae have reported poor performance associated with quantitative lipid imbalances in the diet. The present study examined the effect of dietary protein:neutral lipid ratio on fatty acid (FA) absorption efficiency and metabolism in larval Senegalese sole. In addition, the effect of lipid class (triolein (TRI) and l-3-phosphatidylcholine-1,2-di-oleoyl (PC)), carbon number and degree of saturation of the labelled NEFA, stearic acid (SA), oleic acid (OA) and DHA) was tested. FA absorption was determined by tube feeding [1-14C]-labelled lipids and NEFA after a single meal of either non-enriched Artemia (NEA) or Artemia enriched on a soyabean oil emulsion (EA), or after feeding these diets over an extended period of time (18 d). The tested dietary protein:lipid ratios had no short-term influence but long-term feeding of a diet higher in neutral lipid (EA) increased lipid accumulation within the gut epithelium and resulted in lower FA absorption (higher label evacuation and lower retention of dietary FA), which may partially explain the trend for lower growth observed with this diet. The lipids and NEFA, showed different digestive and metabolic properties, independent of feeding regime. FA absorption increased with unsaturation, being lowest for SA, followed by OA, and highest for DHA. In addition, sole larvae had a lower capacity to digest and absorb FA esterified to TRI, compared with PC, with the order of decreasing absorption being NEFA>PC>>TRI. Moreover, larvae appeared to discriminate between the source of OA, as this FA in the free form or esterified to PC was catabolised less than TRI.
- Published
- 2005
- Full Text
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48. Pathways for urea production during early life of an air-breathing teleost, the African catfish Clarias gariepinus Burchell.
- Author
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Terjesen BF, Chadwick TD, Verreth JA, Rønnestad I, and Wright PA
- Subjects
- Air, Amidine-Lyases metabolism, Amidohydrolases metabolism, Animals, Arginase metabolism, Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) metabolism, Carbon-Nitrogen Ligases metabolism, Catfishes growth & development, Glutamate-Ammonia Ligase metabolism, Kinetics, Larva metabolism, Respiration, Urate Oxidase metabolism, Ureohydrolases metabolism, Catfishes embryology, Catfishes metabolism, Urea metabolism
- Abstract
Embryos and larvae of the African catfish Clarias gariepinus excrete significant quantities of urea. The present study focused on the potential urea-generating pathways during early development of this teleost; uricolysis, argininolysis and the ornithine-urea cycle (OUC). Uricase, allantoinase, allantoicase and ureidoglycollate lyase of the uricolytic pathway were expressed in all early life stages and in adult liver of C. gariepinus. Uricase activity increased in starved larvae compared with yolk-sac larvae. The key regulatory enzyme of the teleost OUC, carbamoyl phosphate synthetase III (CPSase III), was expressed predominantly in muscle of developing C. gariepinus larvae and showed negligible activity in the absence of its allosteric effector N-acetyl-L-glutamate. CPSase III and ornithine carbamoyl transferase activities increased in fed larvae compared with starved larvae. In contrast to the early developmental stages, adult C. gariepinus expressed only low and variable levels of CPSase III, suggesting that, under the experimental conditions employed, OUC expression is influenced by developmental stage in this species. The data indicate that early C. gariepinus life stages express the enzymes necessary for urea production by uricolysis, argininolysis and the OUC, and this may explain why urea tissue levels and urea excretion rates are substantial during the early development of this air-breathing teleost.
- Published
- 2001
- Full Text
- View/download PDF
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