22 results on '"Pingping Gan"'
Search Results
2. Gancao (Glycyrrhizae Radix) provides the main contribution to Shaoyao-Gancao decoction on enhancements of CYP3A4 and MDR1 expression via pregnane X receptor pathway in vitro
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Dandan Feng, Tao Tang, Rong Fan, Jiekun Luo, Hanjin Cui, Yang Wang, and Pingping Gan
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Shaoyao Gancao decoction ,Pregnane X receptor ,Cytochrome P450 3A4 ,Multidrug resistance protein 1 ,Traditional Chinese medicine ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Chinese herbal formula Shaoyao Gancao decoction (SGD) is often used as an adjuvant with chemotherapeutic agents to treat cancer. Due to the herb-drug interactions, the alternations of drug metabolic enzyme and drug transporters induced by SGD deserve to be explored. We aimed to investigate the effect of SGD on the pregnane X receptor (PXR)-mediated transcriptional regulation of cytochrome P450 3A4 (CYP3A4) and drug transporter multidrug resistance protein 1 (MDR1) in vitro. Besides, we assessed the contribution of constituent herbs to SGD on the regulation of CYP3A4 and MDR1. Methods The dual luciferase reporter gene system containing the hPXR expression plasmid and the reporter gene plasmid of CYP3A4 or MDR1 was co-transfected to HepG2 and Caco2 cells. Luciferase activities were determined using a Dual-luciferase reporter assay kit. The gene expression of CYP3A4 and MDR1 in the hPXR-transfected LS174T cells were assessed by real-time qPCR. Finally, the contribution of constituent herbs from SGD was evaluated. Results SGD, Shaoyao and Gancao concentration-dependently increased promoter activities of CYP3A4 and MDR1 in vitro. Moreover, SGD, Shaoyao and Gancao up-regulated CYP3A4 and MDR1 mRNA in hPXR-transfected LS174T cells. As the herbal constituent of SGD, Gancao possesses significantly higher levels of metabolic enzyme and drug transporters compared with Shaoyao. Conclusion SGD tends to enhance CYP3A4 and MDR1 expression via PXR pathway, especially Gancao provides the main contribution. This study highlights a potential in vitro mechanism for SGD on the regulation of drug metabolic enzymes and drug transporters. more...
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- 2018
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Catalog
3. Author Correction: Directed self-assembly of herbal small molecules into sustained release hydrogels for treating neural inflammation
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Jun Zheng, Rong Fan, Huiqiong Wu, Honghui Yao, Yujie Yan, Jiamiao Liu, Lu Ran, Zhifang Sun, Lunzhao Yi, Li Dang, Pingping Gan, Piao Zheng, Tilong Yang, Yi Zhang, Tao Tang, and Yang Wang
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Science - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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- 2020
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4. Shaoyao Gancao Decoction Ameliorates Paclitaxel-Induced Peripheral Neuropathy via Suppressing TRPV1 and TLR4 Signaling Expression in Rats
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Yu, Chen, Ruohuang, Lu, Yang, Wang, and Pingping, Gan
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Pharmacology ,Drug Design, Development and Therapy ,Drug-Related Side Effects and Adverse Reactions ,Paclitaxel ,Peripheral Nervous System Diseases ,TRPV Cation Channels ,Pharmaceutical Science ,Rats ,Toll-Like Receptor 4 ,Hyperalgesia ,Myeloid Differentiation Factor 88 ,Drug Discovery ,Animals ,Drugs, Chinese Herbal - Abstract
Yu Chen,1 Ruohuang Lu,2 Yang Wang,3 Pingping Gan1 1Department of Oncology, Xiangya Hospital, Central South University, Changsha, Peopleâs Republic of China; 2Department of Stomatology, Third Xiangya Hospital, Central South University, Changsha, Peopleâs Republic of China; 3Department of Integrated Traditional Chinese and Western Medicine, Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, Peopleâs Republic of ChinaCorrespondence: Pingping Gan, Department of Oncology, Xiangya Hospital, Central South University, Changsha, Peopleâs Republic of China, Tel +86 13874975101, Email gppxy@csu.edu.cnPurpose: Paclitaxel-induced peripheral neuropathy (PIPN) is increasingly becoming one of the most widespread adverse effects in the treatment of cancer patients, and further precipitate neuroinflammation in the nervous system. Interestingly, Shaoyao Gancao Decoction (SGD), a traditional Chinese analgesic prescription, has emerged as a primary adjuvant to chemotherapy in relieving side effects, especially in the case of PIPN. However, the underlying mechanism of SGD functioning in PIPN remains elusive. Accordingly, the current study set out to explore the potential axis implicated in the functioning of SGD in PIPN.Methods: First, network pharmacology was adopted to predict the role of the transient receptor potential vanilloid type 1 (TRPV1) protein in treating PIPN with SGD. Subsequently, the effects of SGD treatment on mechanical allodynia and thermal hyperalgesia were evaluated in rat PIPN models. Based on the bioinformatics information and current literature, paclitaxel activates toll-like receptor 4 (TLR4) induces the sensitization of TRPV1 mechanistically. Thereafter, TLR4-myeloid-differentiation response gene 88 (MyD88) signaling and TRPV1 expression patterns in dorsal root ganglias (DRGs) were measured by means of Western blotting, qPCR and immunofluorescence.Results: Initial bioinformatics reared a total of 105 bioactive compounds and 1075 target genes from SGD. In addition, 40 target genes intersected with PIPN were considered as potential therapeutic genes. Based on the network analysis, SGD was found to exert its analgesic effect by reducing the expression of TRPV1. Further experimentation validated that SGD exerted an analgesic effect on thermal hyperalgesia in PIPN models, such that this protective effect was associated with the suppression of TRPV1 and TLR4-MyD88 Signaling over-expression.Conclusion: Collectively, our findings indicated that SGD ameliorates PIPN by inhibiting the over-expression of TLR4-MyD88 Signaling and TRPV1, and further highlights the use of SGD as a potential alternative treatment for PIPN.Keywords: paclitaxel-induced peripheral neuropathy, Shaoyao Gancao Decoction, transient receptor potential vanilloid type 1, network pharmacology, paclitaxel, toll-like receptor 4 more...
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- 2022
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5. Study on the Mechanism of Microparticle-Initiated Breakdown in the Air Gap
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Pingping Gan, Yuanjie Shi, Wei Zhong, and Ao Xu
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Nuclear and High Energy Physics ,Materials science ,Plasma ,Condensed Matter Physics ,01 natural sciences ,Cathode ,010305 fluids & plasmas ,law.invention ,Electric discharge in gases ,law ,Electric field ,0103 physical sciences ,Breakdown voltage ,Atomic physics ,Microparticle ,Air gap (plumbing) ,Voltage - Abstract
The microparticle-initiated breakdown plays a key role in the failure of gas switches and has become an active area of investigation in gas discharge recently. The mechanism of the microparticle-initiated breakdown in a 2-mm air gap is studied by experiments and simulations in this article. The dynamics of the microparticle in the electric field is obtained, and four breakdown modes are acquired from the picture of the high-speed camera. The probabilities of breakdown for the 200-, 300-, and 500- $\mu \text{m}$ Cu microparticles under different voltages are presented, and the corresponding lowest breakdown voltages reduce to 65%, 56%, and 47% of the static-breakdown voltage. The relation between the breakdown voltage and position of the microparticle is calculated based on the Townsend theory. The simulated results agree well with the experimental results. The breakdown process and evolution of the plasma are also revealed with PIC simulations. more...
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- 2021
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6. Sharenting in an Evolving Digital World: Increasing Online Connection and Consumer Vulnerability
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L. Lin Ong, Alexa K. Fox, Laurel Aynne Cook, Claire Bessant, Pingping Gan, Mariea Grubbs Hoy, Emma Nottingham, Beatriz Pereira, and Stacey Barell Steinberg
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M900 ,Sociology and Political Science ,M100 ,General Economics, Econometrics and Finance - Abstract
Sharenting (using social media to share content about one’s child) is a progressively common phenomenon enabled by society’s increased connection to digital technology. Although it can encourage positive connections to others, it also creates concerns related to children’s privacy and well-being. In this paper, we establish boundaries and terminology related to sharenting in an evolving digital world. We conceptualize a modern sharenting ecosystem involving key stakeholders (parents, children, community, commercial institutions, and policymakers), by applying consumer vulnerability theory to explore the increased online connection that occurs as work, school, and socialization become increasingly more virtual. Next, we expand the characterization of sharenting by introducing a spectrum of sharenting awareness that categorizes three types of sharenting (active, passive, and invisible). Finally, we provide a research agenda for policymakers and consumer welfare researchers. more...
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- 2022
7. Systematic Analysis of tRNA-Derived Small RNAs Reveals the Effects of Xuefu-Zhuyu Decoction on the Hippocampi of Rats after Traumatic Brain Injury
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Feng Dai, Tao Tang, Ruohuang Lu, Pengfei Li, Dandan Feng, Mingrui Hu, Yang Wang, and Pingping Gan
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Article Subject ,Complementary and alternative medicine - Abstract
Background. Traumatic brain injury (TBI) is one of the most common neurosurgical diseases and refers to brain function impairment or brain pathological changes induced by external causes. A traditional Chinese medicine, Xuefu-Zhuyu Decoction (XFZYD), has been indicated to harbor therapeutic properties against TBI. Transfer RNA (tRNA)-derived small RNAs, that is, tsRNAs (a group of small RNAs derived from tRNAs), are multifunctional regulatory noncoding RNAs generated under pressure and implicated in the progression of TBI. Methods. A TBI model was successfully constructed using rats. We further performed sequencing and omics analyses to identify novel tsRNAs as drug targets for XFZYD therapy against TBI in the rat hippocampus. qPCR assays were used to further verify the experimental results. Gene Ontology (GO) was used to analyze the signaling pathways of downstream target genes of tsRNAs in the XFZYD-regulated TBI model. qPCR was used to detect the influence of overexpressed tsRNA mimics/inhibitors on their target genes in PC12 cells. Results. Our RNA-Seq data illustrate that 11 tsRNAs were mediated by XFZYD. The experimental data revealed AS-tDR-002004 and AS-tDR-002583 as potential targets for XFZYD therapy and showed that they influenced TBI via the cadherin signaling pathway, cocaine addiction, circadian entrainment, and the nicotine pharmacodynamics pathway. We also confirmed that Pi4kb, Mlh3, Pcdh9, and Ppp1cb were target genes of 2 XFZYD-regulated tsRNAs in the hippocampus of a rat model and PC12 cells. Furthermore, biological function analysis revealed the potential therapeutic effects of tsRNAs, and the results showed that Mapk1 and Gnai1 were related genes for XFZYD therapy against TBI. Conclusion. Our work successfully illuminates the efficiency of XFZYD in the treatment of TBI. The experimental data revealed AS-tDR-002004 and AS-tDR-002583 as potential targets for XFZYD therapy and showed that they influenced TBI via the cadherin signaling pathway, cocaine addiction, circadian entrainment, and the nicotine pharmacodynamics pathway in a TBI rat model. more...
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- 2021
8. Systematic Analysis of tRNA-Derived Small RNAs Reveals Effects of Xuefu-Zhuyu Decoction on Hippocampus of Rats after Traumatic Brain Injury
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Mingrui Hu, Feng Dai, Yang Wang, Dandan Feng, Tao Tang, Ruohuang Lu, Pengfei Li, and Pingping Gan
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nervous system ,Traumatic brain injury ,Transfer RNA ,medicine ,Hippocampus ,Decoction ,Xuefu Zhuyu ,Pharmacology ,Biology ,medicine.disease - Abstract
Background: Traumatic brain injury (TBI) is one of the most common neurosurgical diseases which refers to brain function impairment or brain pathological changes induced by external causes. A traditional Chinese medicine, Xuefu Zhuyu Decoction (XFZYD), has been indicated to harbor therapeutic property against TBI. Transfer RNA (tRNA)-derived small RNAs i.e., tsRNAs (a group of small RNAs derived from tRNAs) are multifunctional regulatory non-coding RNAs generated under pressure and implicated in the progression of TBI.Methods: TBI model was successfully constructed by using of rats. Further using sequencing and omics to identify novel tsRNAs as drug targets for XFZYD therapy against TBI in rat hippocampus. qPCR assay was used to further verify the experimental results. GO analyzed the signaling pathways of downstream target genes of tsRNA in XFZYD regulated TBI model. qPCR was used to detect the influence of over-expressed tsRNA mimic/inhibitor on their target genes in PC12 cell.Results: Our RNA-Seq data illustrates that 11 tsRNAs were mediated by the XFZYD. The experimental data revealed AS-tDR-002004 and AS-tDR-002583 as potential targets for XFZYD therapy and influenced TBI via the cadherin signaling pathway, cocaine addiction, circadian entrainment and nicotine pharmacodynamics pathway. We also confirm that Pi4kb, Mlh3, Pcdh9, and Ppp1cb were targets genes of 2 XFZYD regulated tsRNAs in hippocampus of rat model and PC12 cells. Furthermore, biological function analysis revealing potential therapeutic effects of tsRNAs, and results found Mapk1, Gnai1 was the related genes of for XFZYD therapy against TBI.Conclusion: Our work successfully illuminates the efficiency of XFZYD for the treatment of TBI. The experimental data revealed AS-tDR-002004 and AS-tDR-002583 as potential targets for XFZYD therapy and influenced TBI via the cadherin signaling pathway, cocaine addiction, circadian entrainment and nicotine pharmacodynamics pathway in TBI rat model. more...
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- 2021
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9. Chaihu-Shugan-San Reinforces CYP3A4 Expression via Pregnane X Receptor in Depressive Treatment of Liver-Qi Stagnation Syndrome
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Pingping Gan, Ruohuang Lu, Xu Liu, Rong Fan, Yang Wang, Jiekun Luo, Hanjin Cui, Ze-Hui He, Chunhu Zhang, and Tao Tang
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0303 health sciences ,Pregnane X receptor ,Article Subject ,CYP3A4 ,medicine.diagnostic_test ,business.industry ,lcsh:Other systems of medicine ,Traditional Chinese medicine ,Pharmacology ,lcsh:RZ201-999 ,03 medical and health sciences ,surgical procedures, operative ,0302 clinical medicine ,Complementary and alternative medicine ,Downregulation and upregulation ,Western blot ,otorhinolaryngologic diseases ,medicine ,Antidepressant ,Luciferase ,business ,CYP2C9 ,030217 neurology & neurosurgery ,Research Article ,030304 developmental biology - Abstract
Backgrounds. Chaihu-Shugan-San (CSS) is a classic traditional Chinese herbal prescription for treating depression. However, the underlying mechanism of the Chinese syndrome-specific efficacy of CSS is poorly understood. Aim of the Study. From traditional Chinese medicine and pharmacogenetics perspectives, the present study aimed to investigate the antidepressant effects of CSS on a mouse model of Liver-Qi Stagnation (LQS) syndrome and its underlying mechanisms. Methods and Materials. We used two main mouse models of depressive syndromes in the study, including LQS and liver stagnation and spleen deficiency (LSSD) syndrome. Tail suspension and forced swimming tests were used to evaluate the effects of CSS on animal behaviour. The expression level of the CYP450 enzyme from liver microsomes was analysed by western blot (WB) analysis and quantitative real-time polymerase chain reaction (qRT-PCR). More specifically, we analysed the key compounds of CSS that are responsible for CYP450 regulation via bioinformatics. Ultimately, luciferase assays were employed to confirm the prediction in vitro. Results. CSS remarkably reduced the immobile time in LQS rather than in LSSD mice. Although CSS significantly upregulated CYP2C9 in mice with both syndromes, activated translation of CYP3A4 induced by CSS was only observed in the LQS group. Bioinformatics analysis revealed that the unique regulation of CYP3A4 was responsible for the effects of glycyrrhetinic acid (GA) from CSS. Further luciferase assays confirmed the enhancement of CYP3A4 expression via the pregnane X receptor (PXR) pathway in vitro. Conclusions. CSS specifically upregulates the translation of CYP3A4 via the PXR pathway in depressed LQS mice. GA, a bioactive compound that originates from CSS, contributes to this activation. This work provides novel insight into Chinese syndrome-based therapy for depression. more...
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- 2019
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10. Novel Functions of CD147 in the Mitochondria Exacerbates Melanoma Metastasis
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Xiang Chen, Jianglin Zhang, Jianda Zhou, Juan Su, Lisha Wu, Pingping Gan, Cong Peng, Xu Zhang, and Lixia Lu
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Male ,China ,Skin Neoplasms ,ATP5B ,Mitochondrion ,Biology ,Applied Microbiology and Biotechnology ,Metastasis ,Cell membrane ,Mitochondrial Proteins ,03 medical and health sciences ,Downregulation and upregulation ,aerobic oxidation ,Cell Line, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,Neoplasm Metastasis ,Molecular Biology ,Melanoma ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,0303 health sciences ,Cell Biology ,Chaperonin 60 ,Middle Aged ,Mitochondrial Proton-Translocating ATPases ,medicine.disease ,invasion ,Prognosis ,Mitochondria ,medicine.anatomical_structure ,Cancer research ,CD147 ,Basigin ,HSP60 ,Female ,Skin cancer ,Developmental Biology ,Research Paper - Abstract
Melanoma is an aggressive form of skin cancer characterized by rapid invasion and metastasis. CD147 is known to be functioning in cell invasion. In this study, we showed that CD147 was translocated from the cell membrane to the mitochondria in advanced melanoma. Melanoma patients with CD147 localized to the mitochondria confer a worse prognosis. The mitochondrial CD147 levels are correlated with the invasion potential of various melanoma cell lines as well as mitochondrial energy metabolism. Depletion of CD147 decreased the activity of mitochondrial complex V. STRING analysis for protein-protein interaction networks (PPIN) in CD147-depleted melanoma cells showed that mitochondrial proteins HSP60 and ATP5B, a subunit of mitochondrial complex V, were node proteins. HSP60 upregulation was correlated with a worse prognosis of melanoma patients. Co-immunoprecipitation (Co-IP) assay indicates that CD147 interacts with HSP60. These data suggested that mitochondrial CD147 may prompt HSP60 to activate ATP5B, thereby promoting the mitochondrial aerobic oxidation and the invasive abilities of melanoma cells. Correlation analysis of the data acquired from patients was helpful to draw a 5-year survival curve for patients who screened positive and negative for mitochondrial CD147. This study unravels the function of CD147 in tumor invasion and highlights it as a potential tumor therapeutic target. more...
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- 2020
11. Metabolomics reveals the effect of Xuefu Zhuyu Decoction on plasma metabolism in rats with acute traumatic brain injury
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Dandan Feng, Zian Xia, Rong Fan, Hongmei Lu, Yang Wang, Tao Tang, Zhiming Wang, Wei Huang, Jing Zhou, Chunhu Zhang, Feng He, Pingping Gan, Hanjin Cui, Xingui Xiong, and Weijun Peng
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,Traumatic brain injury ,Xuefu Zhuyu Decoction ,Decoction ,acute traumatic brain injury ,Traditional Chinese medicine ,Neuroprotection ,gas chromatography/mass spectrometry ,traditional Chinese medicine ,03 medical and health sciences ,0302 clinical medicine ,Metabolomics ,Internal medicine ,medicine ,Xuefu Zhuyu ,Traditional medicine ,business.industry ,Plasma Metabolism ,medicine.disease ,metabolomics ,030104 developmental biology ,business ,030217 neurology & neurosurgery ,Research Paper ,Western medicine - Abstract
// Dandan Feng 1 , Zian Xia 1 , Jing Zhou 1 , Hongmei Lu 2 , Chunhu Zhang 1 , Rong Fan 1 , Xingui Xiong 1 , Hanjin Cui 1 , Pingping Gan 3 , Wei Huang 1 , Weijun Peng 4 , Feng He 5 , Zhiming Wang 5 , Yang Wang 1 and Tao Tang 1 1 Institute of Integrative Chinese Medicine, Xiangya Hospital, Central South University, Changsha 410008, P.R. China 2 Research Center of Modernization of Traditional Chinese Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P.R. China 3 Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, P.R. China 4 Department of Integrated Chinese and Western Medicine, The Second Xiangya Hospital, Central South University, Changsha 410011, P.R. China 5 Department of Hepatobiliary Surgery, Xiangya Hospital, Central South University, Changsha 410008, P.R. China Correspondence to: Yang Wang, email: wangyang_xy87@csu.edu.cn Tao Tang, email: falcontang@126.com Keywords: metabolomics, acute traumatic brain injury, traditional Chinese medicine, Xuefu Zhuyu Decoction, gas chromatography/mass spectrometry Received: February 21, 2017 Accepted: September 03, 2017 Published: October 16, 2017 ABSTRACT Xuefu Zhuyu Decoction (XFZY), an important traditional Chinese herbal formula, has been reported effective on traumatic brain injury (TBI) in rats. However, its cerebral protection mechanism has not been clarified at the metabolic level. This work aims to explore the global metabolic characteristics of XFZY in rats during the acute phase of TBI on days 1 and 3. A plasma metabolomics method based on gas chromatography-mass spectrometry coupled with univariate analysis and multivariate statistical analysis was performed in three groups (Sham, Vehicle, XFZY). Then, a pathway analysis using MetaboAnalyst 3.0 was performed to illustrate the pathways of therapeutic action of XFZY in TBI. XFZY treatment attenuates neurological dysfunction and cortical lesion volume post-injury on day 3, and reverses the plasma metabolite abnormalities (glutamic acid, lactic acid, 3-hydroxybutyric acid, and ribitol, etc.). These differential metabolites are mainly involved in D-glutamine and D-glutamate metabolism, alanine, aspartate and glutamate metabolism, and inositol phosphate metabolism. Our study reveals potential biomarkers and metabolic networks of acute TBI and neuroprotection effects of XFZY, and shows this metabolomics approach with MetaboAnalyst would be a feasible way to systematically study therapeutic effects of XFZY on TBI. more...
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- 2017
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12. TUSC3 induces autophagy in human non-small cell lung cancer cells through Wnt/β-catenin signaling
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Jun Cao, Xiao-Yi Yao, Jian-Huang Li, Meizuo Zhong, Yun Peng, Pingping Gan, and Jian-Xin Wang
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0301 basic medicine ,Pathology ,medicine.medical_specialty ,autophagy ,Wnt/β-catenin signaling pathway ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,A549 cells ,tumor suppressor candidate 3 ,Lung cancer ,non-small cell lung cancer ,A549 cell ,business.industry ,Cell growth ,Autophagy ,Wnt signaling pathway ,Cancer ,medicine.disease ,respiratory tract diseases ,030104 developmental biology ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,Signal transduction ,business ,Research Paper - Abstract
// Yun Peng 1 , Jun Cao 2 , Xiao-Yi Yao 3 , Jian-Xin Wang 4 , Mei-Zuo Zhong 3 , Ping-Ping Gan 3 and Jian-Huang Li 3 1 International Medical Center, Xiangya Hospital, Central South University, Changsha 410008, P.R. China 2 Department of Medical Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410008, P.R. China 3 Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, P.R. China 4 School of Information Science and Engineering, Central South University, Changsha 410008, P.R. China Correspondence to: Jian-Huang Li, email: jianhuang_l@126.com Keywords: non-small cell lung cancer, A549 cells, tumor suppressor candidate 3, Wnt/β-catenin signaling pathway, autophagy Received: October 31, 2016 Accepted: March 19, 2017 Published: May 08, 2017 ABSTRACT We investigated the effects of tumor suppressor candidate 3 ( TUSC3 ) on autophagy in human non-small cell lung cancer (NSCLC) cells. A total of 118 NSCLC patients (88 males and 30 females) who underwent surgery at our institute were enrolled in the study. Immunohistochemical analysis revealed that TUSC3 protein expression was lower in NSCLC specimens than adjacent normal tissue. Correspondingly, there was greater methylation of TUSC3 in NSCLC than adjacent normal tissue. After transient transfection of A549 NSCLC cells with constructs designed to up-regulate or down-regulate TUSC3 expression, we analyzed the effects of inhibiting the Wnt pathway (XAV939) and autophagy (chloroquine, CQ) on the behavior of NSCLC cells. We also performed TOP/FOP-Flash reporter assays, MTT assays, Annexin V-FITC/propidium iodide staining, and acridine orange staining to evaluate Wnt/β-catenin signaling, cell proliferation, apoptosis, and autophagy, respectively. Expression of Wnt/β-catenin pathway components and autophagy-related proteins was analyzed using qRT-PCR and Western blotting. We found that TUSC3 inhibited cell proliferation and promoted both apoptosis and autophagy in A549 cells. In addition, TUSC3 increased expression of autophagy-related proteins. It also increased expression of Wnt/β-catenin signaling pathway components and promoted nuclear transfer of β-catenin, resulting in activation of Wnt/β-catenin signaling. TUSC3 thus induces autophagy in human NSCLC cells through activation of the Wnt/β-catenin signaling pathway. more...
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- 2017
13. Hydroxysafflor yellow A exerts antioxidant effects in a rat model of traumatic brain injury
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Yafei Shi, Wei Huang, Chunhu Zhang, Pingping Gan, Weijun Peng, Zian Xia, Yang Wang, and Rong Fan
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Male ,0301 basic medicine ,Cancer Research ,Antioxidant ,medicine.medical_treatment ,Carthamus tinctorius ,Pharmacology ,medicine.disease_cause ,Biochemistry ,Antioxidants ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Chalcone ,0302 clinical medicine ,Malondialdehyde ,Brain Injuries, Traumatic ,biology ,traumatic brain injury ,Quinones ,Brain ,Articles ,Catalase ,hydroxysafflor yellow A ,Oncology ,Molecular Medicine ,Traumatic brain injury ,Superoxide dismutase ,03 medical and health sciences ,Genetics ,medicine ,Animals ,Molecular Biology ,Superoxide Dismutase ,Glutathione ,medicine.disease ,Molecular biology ,neuroprotective effect ,ultra performance liquid chromatography-tandem mass spectrometry ,Rats ,nervous system diseases ,Disease Models, Animal ,Oxidative Stress ,030104 developmental biology ,chemistry ,biology.protein ,Glutathione disulfide ,030217 neurology & neurosurgery ,Oxidative stress - Abstract
Free radical-induced oxidative damage occurs rapidly and is of primary importance during the secondary pathophysiological cascades of traumatic brain injury (TBI). Hydroxysafflor yellow A (HSYA) is a constituent of the flower petals of Carthamus tinctorius (safflower) and may represent a potential therapeutic strategy to improve outcomes following TBI. The present study aimed to identify HSYA in the brain tissues of rats exposed to TBI to determine its absorption and to investigate the underlying effects of HSYA on antioxidant enzymes in the brain tissues of TBI rats. To determine the absorption of HSYA for the investigation of the underlying antioxidant effects of HSYA in TBI, the presence of HSYA in the brain tissues of the TBI rats was identified using an ultra performance liquid chromatography‑tandem mass spectrometry method. Subsequently, the state of oxidative stress in the TBI rat model following the administration of HSYA was investigated by determining the levels of antioxidant enzymes, including superoxide dismutase (SOD), malondialdehyde (MDA) and catalase (CAT), and the ratio of glutathione (GSH)/glutathione disulfide (GSSG). The data obtained demonstrated that HSYA was absorbed in the brain tissues of the TBI rats. HSYA increased the activities of SOD and CAT, the level of GSH and the GSH/GSSG ratio. However, HSYA concomitantly decreased the levels of MDA and GSSG. These preliminary data suggest that HSYA has the potential to be utilized as a neuroprotective drug in cases of TBI. more...
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- 2016
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14. Author Correction: Directed self-assembly of herbal small molecules into sustained release hydrogels for treating neural inflammation
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Tao Tang, Yang Wang, Li Dang, Huiqiong Wu, Yi Zhang, Lu Ran, Yujie Yan, Jiamiao Liu, Lunzhao Yi, Jun Zheng, Rong Fan, Honghui Yao, Pingping Gan, Tilong Yang, Zhifang Sun, and Piao Zheng
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Directed self assembly ,Multidisciplinary ,Chemistry ,Science ,technology, industry, and agriculture ,General Physics and Astronomy ,Inflammation ,General Chemistry ,Pharmacology ,Small molecule ,complex mixtures ,General Biochemistry, Genetics and Molecular Biology ,Article ,Drug delivery ,Self-healing hydrogels ,medicine ,lcsh:Q ,medicine.symptom ,lcsh:Science - Abstract
Self-assembling natural drug hydrogels formed without structural modification and able to act as carriers are of interest for biomedical applications. A lack of knowledge about natural drug gels limits there current application. Here, we report on rhein, a herbal natural product, which is directly self-assembled into hydrogels through noncovalent interactions. This hydrogel shows excellent stability, sustained release and reversible stimuli-responses. The hydrogel consists of a three-dimensional nanofiber network that prevents premature degradation. Moreover, it easily enters cells and binds to toll-like receptor 4. This enables rhein hydrogels to significantly dephosphorylate IκBα, inhibiting the nuclear translocation of p65 at the NFκB signalling pathway in lipopolysaccharide-induced BV2 microglia. Subsequently, rhein hydrogels alleviate neuroinflammation with a long-lasting effect and little cytotoxicity compared to the equivalent free-drug in vitro. This study highlights a direct self-assembly hydrogel from natural small molecule as a promising neuroinflammatory therapy., There is interest in the development of drug-based hydrogels for responsive sustained drug release. Here, the authors report on the self-assembly of natural small molecule, rhein, into hydrogels and the application of the hydrogels as stable controlled release agents for neuro-inflammatory therapy more...
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- 2020
15. Gancao (Glycyrrhizae Radix) provides the main contribution to Shaoyao-Gancao decoction on enhancements of CYP3A4 and MDR1 expression via pregnane X receptor pathway in vitro
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Yang Wang, Pingping Gan, Hanjin Cui, Rong Fan, Dandan Feng, Tao Tang, and Jiekun Luo
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0301 basic medicine ,Pharmacology ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Traditional Chinese medicine ,Cytochrome P450 3A4 ,Gene expression ,Multidrug resistance protein 1 ,Transcriptional regulation ,Glycyrrhiza ,Cytochrome P-450 CYP3A ,Humans ,Luciferase ,Drug Interactions ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Reporter gene ,Pregnane X receptor ,Expression vector ,CYP3A4 ,Chemistry ,Plant Extracts ,Pregnane X Receptor ,Transporter ,lcsh:Other systems of medicine ,General Medicine ,lcsh:RZ201-999 ,030104 developmental biology ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Shaoyao Gancao decoction ,Research Article ,Drugs, Chinese Herbal - Abstract
Background Chinese herbal formula Shaoyao Gancao decoction (SGD) is often used as an adjuvant with chemotherapeutic agents to treat cancer. Due to the herb-drug interactions, the alternations of drug metabolic enzyme and drug transporters induced by SGD deserve to be explored. We aimed to investigate the effect of SGD on the pregnane X receptor (PXR)-mediated transcriptional regulation of cytochrome P450 3A4 (CYP3A4) and drug transporter multidrug resistance protein 1 (MDR1) in vitro. Besides, we assessed the contribution of constituent herbs to SGD on the regulation of CYP3A4 and MDR1. Methods The dual luciferase reporter gene system containing the hPXR expression plasmid and the reporter gene plasmid of CYP3A4 or MDR1 was co-transfected to HepG2 and Caco2 cells. Luciferase activities were determined using a Dual-luciferase reporter assay kit. The gene expression of CYP3A4 and MDR1 in the hPXR-transfected LS174T cells were assessed by real-time qPCR. Finally, the contribution of constituent herbs from SGD was evaluated. Results SGD, Shaoyao and Gancao concentration-dependently increased promoter activities of CYP3A4 and MDR1 in vitro. Moreover, SGD, Shaoyao and Gancao up-regulated CYP3A4 and MDR1 mRNA in hPXR-transfected LS174T cells. As the herbal constituent of SGD, Gancao possesses significantly higher levels of metabolic enzyme and drug transporters compared with Shaoyao. Conclusion SGD tends to enhance CYP3A4 and MDR1 expression via PXR pathway, especially Gancao provides the main contribution. This study highlights a potential in vitro mechanism for SGD on the regulation of drug metabolic enzymes and drug transporters. more...
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- 2018
16. Directed self-assembly of herbal small molecules into sustained release hydrogels for treating neural inflammation
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Yujie Yan, Lunzhao Yi, Piao Zheng, Yi Zhang, Jun Zheng, Zhifang Sun, Pingping Gan, Tao Tang, Honghui Yao, Lu Ran, Jiamiao Liu, Yang Wang, Li Dang, Rong Fan, Huiqiong Wu, and Tilong Yang
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0301 basic medicine ,Lipopolysaccharides ,Phytochemicals ,Anti-Inflammatory Agents ,Drug Evaluation, Preclinical ,Nanofibers ,General Physics and Astronomy ,Anthraquinones ,02 engineering and technology ,chemistry.chemical_compound ,Mice ,NF-KappaB Inhibitor alpha ,Phosphorylation ,Cytotoxicity ,lcsh:Science ,Drug Carriers ,Multidisciplinary ,Chemistry ,Small molecules ,Hydrogels ,Self-assembly ,021001 nanoscience & nanotechnology ,Small molecule ,Self-healing hydrogels ,Microglia ,0210 nano-technology ,Gels and hydrogels ,Signal Transduction ,Science ,macromolecular substances ,complex mixtures ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,03 medical and health sciences ,Animals ,Rheum ,Author Correction ,Neuroinflammation ,Inflammation ,Natural product ,technology, industry, and agriculture ,Transcription Factor RelA ,General Chemistry ,In vitro ,Toll-Like Receptor 4 ,IκBα ,Drug Liberation ,030104 developmental biology ,Nanofiber ,Delayed-Action Preparations ,Drug delivery ,Biophysics ,Microscopy, Electron, Scanning ,lcsh:Q ,Biomedical materials - Abstract
Self-assembling natural drug hydrogels formed without structural modification and able to act as carriers are of interest for biomedical applications. A lack of knowledge about natural drug gels limits there current application. Here, we report on rhein, a herbal natural product, which is directly self-assembled into hydrogels through noncovalent interactions. This hydrogel shows excellent stability, sustained release and reversible stimuli-responses. The hydrogel consists of a three-dimensional nanofiber network that prevents premature degradation. Moreover, it easily enters cells and binds to toll-like receptor 4. This enables rhein hydrogels to significantly dephosphorylate IκBα, inhibiting the nuclear translocation of p65 at the NFκB signalling pathway in lipopolysaccharide-induced BV2 microglia. Subsequently, rhein hydrogels alleviate neuroinflammation with a long-lasting effect and little cytotoxicity compared to the equivalent free-drug in vitro. This study highlights a direct self-assembly hydrogel from natural small molecule as a promising neuroinflammatory therapy. more...
- Published
- 2018
17. Silver Ångstrom‐Particles: Ångstrom‐Scale Silver Particles as a Promising Agent for Low‐Toxicity Broad‐Spectrum Potent Anticancer Therapy (Adv. Funct. Mater. 23/2019)
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Hao Yin, Rong Fan, Ze Long, Ze-Hui He, Ben Wu, Jie Huang, Xiong-Ke Hu, Zheng-Zhao Liu, Shan-Shan Rao, Situ Weiyi, Yang Wang, Tuan-Hui Chen, Yi-Juan Tan, Yi-Yi Wang, Kun Xia, Hui Xie, Zhong-Wei Luo, Chun-Yuan Chen, Zhen-Xing Wang, Yi-Wei Liu, Chun-Gu Hong, Hao-Ming Liu, Fuxingzi Li, Ling-Qing Yuan, Ming-Jie Luo, Jia Cao, and Pingping Gan more...
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Biomaterials ,Broad spectrum ,Materials science ,Low toxicity ,Scale (ratio) ,Electrochemistry ,Nanotechnology ,Angstrom ,Condensed Matter Physics ,Silver particles ,Electronic, Optical and Magnetic Materials - Published
- 2019
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18. Novel Functions of CD147 in the Mitochondria Exacerbates Melanoma Metastasis.
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Lixia Lu, Jianglin Zhang, Pingping Gan, Lisha Wu, Xu Zhang, Cong peng, Jianda Zhou, Xiang Chen, and Juan Su
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- 2021
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19. Rhein and rhubarb similarly protect the blood-brain barrier after experimental traumatic brain injury via gp91phox subunit of NADPH oxidase/ROS/ERK/MMP-9 signaling pathway
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Weijun Peng, Yang Wang, Xi Huang, Wei Huang, Xuegong Fan, Chunhu Zhang, Pingping Gan, Zebing Huang, Xingui Xiong, Tao Tang, and Rong Fan
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0301 basic medicine ,MAPK/ERK pathway ,Male ,MAP Kinase Signaling System ,Anthraquinones ,Brain Edema ,Pharmacology ,Blood–brain barrier ,medicine.disease_cause ,Article ,Antioxidants ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Western blot ,In vivo ,Brain Injuries, Traumatic ,medicine ,Animals ,Rheum ,Evans Blue ,Multidisciplinary ,NADPH oxidase ,medicine.diagnostic_test ,biology ,Blot ,Oxidative Stress ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Matrix Metalloproteinase 9 ,Blood-Brain Barrier ,NADPH Oxidase 2 ,biology.protein ,Zonula Occludens-1 Protein ,Reactive Oxygen Species ,030217 neurology & neurosurgery ,Oxidative stress ,Drugs, Chinese Herbal ,Signal Transduction - Abstract
Oxidative stress chiefly contributes to the disruption of the BBB following traumatic brain injury (TBI). The Chinese herbal medicine rhubarb is a promising antioxidant in treating TBI. Here we performed in vivo and in vitro experiments to determine whether rhubarb and its absorbed bioactive compound protected the BBB after TBI by increasing ZO-1 expression through inhibition of gp91phox subunit of NADPH oxidase/ROS/ERK/MMP-9 pathway. Rats were subjected to the controlled cortical impact (CCI) model, and primary rat cortical astrocytes were exposed to scratch-wound model. The liquid chromatography with tandem mass spectrometry method showed that rhein was the compound absorbed in the brains of CCI rats after rhubarb administration. The wet-dry weights and Evans blue measurements revealed that rhubarb and rhein ameliorated BBB damage and brain edema in CCI rats. Western blots showed that rhubarb and rhein downregulated GFAP in vitro. RT-PCR, immunohistochemistry, Western blot and dichlorodihydrofluorescein diacetate analysis indicated that rhubarb prevented activation of gp91phox subunit of NADPH oxidase induced ROS production, subsequently inhibited ERK/MMP-9 pathway in vivo and in vitro. Interestingly, rhein and rhubarb similarly protected the BBB by inhibiting this signaling cascade. The results provide a novel herbal medicine to protect BBB following TBI via an antioxidative molecular mechanism. more...
- Published
- 2016
20. Xuefu Zhuyu decoction, a traditional Chinese medicine, provides neuroprotection in a rat model of traumatic brain injury via an anti-inflammatory pathway
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Weiping Liu, Zian Xia, Xingui Xiong, Jun Qi Li, Weijun Peng, Chunyan Fu, Yong Zou, Pingping Gan, Yang Wang, Jiekun Luo, Chenxia Sheng, Rong Fan, Tao Tang, Chunhu Zhang, Wei Huang, and Zhihua Xing
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Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Traumatic brain injury ,Anti-Inflammatory Agents ,Morris water navigation task ,Inflammation ,Traditional Chinese medicine ,Pharmacology ,Neuroprotection ,Gas Chromatography-Mass Spectrometry ,Article ,03 medical and health sciences ,Cognition ,0302 clinical medicine ,Memory ,Brain Injuries, Traumatic ,medicine ,Animals ,Medicine, Chinese Traditional ,Phosphorylation ,Maze Learning ,Protein kinase B ,Neuroinflammation ,PI3K/AKT/mTOR pathway ,Arachidonic Acid ,Multidisciplinary ,business.industry ,TOR Serine-Threonine Kinases ,Ribosomal Protein S6 Kinases, 70-kDa ,medicine.disease ,Rats ,Disease Models, Animal ,Neuroprotective Agents ,030104 developmental biology ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Cytokines ,Inflammation Mediators ,medicine.symptom ,business ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery ,Chromatography, Liquid ,Drugs, Chinese Herbal ,Signal Transduction - Abstract
Neuroinflammation is central to the pathology of traumatic brain injury (TBI). Xuefu Zhuyu decoction (XFZY) is an effective traditional Chinese medicine to treat TBI. To elucidate its potential molecular mechanism, this study aimed to demonstrate that XFZY functions as an anti-inflammatory agent by inhibiting the PI3K-AKT-mTOR pathway. Sprague-Dawley rats were exposed to controlled cortical impact to produce a neuroinflammatory response. The treatment groups received XFZY (9 g/kg and 18 g/kg), Vehicle group and Sham group were gavaged with equal volumes of saline. The modified neurologic severity score (mNSS) and the Morris water maze test were used to assess neurological deficits. Arachidonic acid (AA) levels in brain tissue were measured using tandem gas chromatography-mass spectrometry. TNF-α and IL-1β levels in injured ipsilateral brain tissue were detected by ELISA. AKT and mTOR expression were measured by western blot analysis. The results indicated that XFZY significantly enhanced spatial memory acquisition. XFZY (especially at a dose of 9 g/kg) markedly reduced the mNSS and levels of AA, TNF-α and IL-1β. Significant downregulation of AKT/mTOR/p70S6K proteins in brain tissues was observed after the administration of XFZY (especially at a dose of 9 g/kg). XFZY may be a promising therapeutic strategy for reducing inflammation in TBI. more...
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- 2016
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21. Diagnostic accuracy of circulating tumor cells detection in gastric cancer: systematic review and meta-analysis.
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Lanhua Tang, Shushan Zhao, Wei Liu, Parchim, Nicholas F., Jin Huang, Youhong Tang, and Pingping Gan
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CANCER cells ,META-analysis ,CANCER diagnosis ,STOMACH cancer ,CONFIDENCE intervals ,TUMOR diagnosis ,DATABASES ,DIAGNOSIS - Abstract
Background: Circulating tumor cells (CTCs) detection has previously been used for diagnosing gastric cancer. However, the previous studies failed to make an agreement whether the detection of CTCs contributes to the diagnosis of gastric cancer. Methods: A systematic review and meta-analysis was performed to evaluate the overall accuracy of CTCs detection for diagnosing gastric cancer. PubMed, Embase and the Wanfang database were searched in all languages published up to Oct 2012. The pooled sensitivity (SEN), specificity (SPE), positive and negative likelihood ratios (PLR and NLR, respectively), diagnostic odds ratio (DOR) and summary receiver operating characteristic (sROC) curve were calculated to evaluate the overall test performance. Results: Twenty studies were included in this systematic review and meta-analysis. The diagnostic value of CTCs detection for the gastric cancer was calculated to evaluate the overall test performance. The summary estimates of The pooled sensitivity, specificity, positive and negative likelihood ratios, diagnostic odds ratio were 0.42 (95% confidence interval (CI), 0.21-0.67), 0.99 (95% CI, 0.96-1.00), 58.2 (95% CI, 9.8-345.9), 0.58 (95% CI, 0.38-0.89), and 100 (95% CI, 15-663), respectively. The summary receiver operating characteristic curve was 0.97 (95% CI, 0.95-0.98). Deek's funnel plot asymmetry test found no evidence of study publication bias in the current study (P = 0.49). Conclusion: This systematic review suggests that CTCs detection alone cannot be recommended as a screening test for gastric cancer. However, it might be used as a noninvasive method for the confirmation of the gastric cancer diagnosis. [ABSTRACT FROM AUTHOR] more...
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- 2013
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22. Enhanced antitumoral efficacy and immune response following conditionally replicative adenovirus containing constitutive HSF1 delivery to rodent tumors.
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Rong Fan, Cheng Wang, Yang Wang, Ping Ren, Pingping Gan, Hui Ji, Zian Xia, Suiyu Hu, Qiongyao Zeng, Wei Huang, Yebin Jiang, and Xi Huang
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HEAT shock proteins ,ADENOVIRUSES ,ANTINEOPLASTIC agents ,COLON cancer ,TRANSCRIPTION factors - Abstract
Background: Oncolytic adenoviruses are promising as anticancer agents but have limited clinical responses. Our previous study showed that heat shock transcription factor 1 (HSF1) overexpression could increase the anti-tumor efficacy of E1B55kD deleted oncolytic adenovirus through increasing the viral burst. Due to the important roles of heat shock proteins (HSPs) in eliciting innate and adaptive immunity, we reasoned that besides increasing the viral burst, HSF1 may also play a role in increasing tumor specific immune response. Methods: In the present study, intra-dermal murine models of melanoma (B16) and colorectal carcinoma (CT26) were treated with E1B55kD deleted oncolytic adenovirus Adel55 or Adel55 incorporated with cHSF1, HSF1i, HSP70, or HSP90 by intra-tumoral injection. Tumors were surgically excised 72 h post injection and animals were analyzed for tumor resistance and survival rate. Results: Approximately 95% of animals in the Adel55-cHSF1 treated group showed sustained resistance upon re-challenge with autologous tumor cells, but not in PBS, Adel55, or Adel55-HSF1i treated groups. Only 50--65% animals in the Adel55-HSP70 and Adel55-HSP90 treated group showed tumor resistance. Tumor resistance was associated with development of tumor type specific cellular immune responses. Adel55-cHSF1 treatment also showed higher efficacy in diminishing progression of the secondary tumor focus than Adel55-HSP70 or Adel55- HSP90 treatment. Conclusions: Besides by increasing its burst in tumor cells, cHSF1 could also augment the potential of E1B55kD deleted oncolytic adenovirus by increasing the tumor-specific immune response, which is beneficial to prevent tumor recurrence. cHSF1 is a better gene for neoadjuvant immunotherapy than other heat shock protein genes. [ABSTRACT FROM AUTHOR] more...
- Published
- 2012
- Full Text
- View/download PDF
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