37 results on '"Petterson T"'
Search Results
2. P1345: IMMUNE RECONSTITUTION AFTER BMT FOR NON-MALIGNANT HEMATOLOGICAL CONDITIONS FOR RELATED, UNRELATED AND HAPLOIDENTICAL DONORS. A STUDY IN THE PEDIATRIC DEPARTMENT AT ST MARY’S HOSPITAL BETWEEN 2019&2020
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Khoder, A., primary, Gassas, A., additional, Bremathas, S., additional, Rabanal, O., additional, Hennesy, K., additional, Loaiza, S., additional, Lund, K., additional, Petterson, T., additional, Karnik, L., additional, and Josu de la Fuente, J., additional more...
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- 2022
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Catalog
3. P656: INCIDENT VENOUS THROMBOEMBOLISM (VTE) IN PATIENTS WITH MONOCLONAL B-CELL LYMPHOCYTOSIS (MBL) AND CHRONIC LYMPHOCYTIC LEUKEMIA (CLL) – A POPULATION-BASED STUDY
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Koehler, A., primary, Moise, M., additional, Call, T., additional, Rabe, K., additional, Achenbach, S., additional, Crusan, D., additional, Bailey, K., additional, Petterson, T., additional, Ding, W., additional, Kenderian, S., additional, Leis, J., additional, Wang, Y., additional, Muchtar, E., additional, Hampel, P., additional, Schwager, S., additional, Slager, S., additional, Kay, N., additional, Ashrani, A., additional, and Parikh, S., additional more...
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- 2022
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4. Risk factors for incident venous thromboembolism in active cancer patients: a population based case-control study: OR128
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Ashrani, A A, Gullerud, R E, Petterson, T M, Marks, R S, Bailey, K R, and Heit, J A
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- 2015
5. Incidence of venous thromboembolism after elective knee arthroscopic surgery: a historical cohort study
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Mauck, K. F., Froehling, D. A., Daniels, P. R., Dahm, D. L., Ashrani, A. A., Crusan, D. J., Petterson, T. M., Bailey, K. R., and Heit, J. A.
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- 2013
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6. A genome-wide association study of venous thromboembolism identifies risk variants in chromosomes 1q24.2 and 9q
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HEIT, J. A., ARMASU, S. M., ASMANN, Y. W., CUNNINGHAM, J. M., MATSUMOTO, M. E., PETTERSON, T. M., and DE ANDRADE, M.
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- 2012
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7. Genetic variation within the anticoagulant, procoagulant, fibrinolytic and innate immunity pathways as risk factors for venous thromboembolism
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HEIT, J. A., CUNNINGHAM, J. M., PETTERSON, T. M., ARMASU, S. M., RIDER, D. N., and DE ANDRADE, M.
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- 2011
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8. Is progesterone an independent risk factor for incident venous thromboembolism (VTE)? a population-based case-control study: AS-TH-003
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Barsoum, M, Heit, J A, Ashrani, A A, Leibson, C L, Petterson, T M, Bailey, K R, and Melton, L J, III
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- 2009
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9. Familial segregation of venous thromboembolism
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Heit, J. A., Phelps, M. A., Ward, S. A., Slusser, J. P., Petterson, T. M., and De Andrade, M.
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- 2004
10. LDH based neonatal diagnostics on a low-cost slipdisc based sample preparation platform
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Banerjee, Indradumna, Salih, Tagrid, Ramachandraiah, Harisha, Erlandsson, J., Petterson, T., Silva, AC, Karlsson, M, and Russom, Aman
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Teknik och teknologier ,Engineering and Technology - Abstract
INTRODUCTION Slipdisc is developed as a sample preparation platform based on slipchip technology [1], using a handwinded clockwork mechanism allowing sample processing from one spot to another with defined precision without the need for sophisticated tools or alignment (Fig.1). An ordinary smartphone or camera can be used to image and analyse the results making it an ideal tool for resource limited settings. Here, we demonstrate a bioassay for detecting LDH (Fig.2), a crucial enzyme found in all living cells which leaks out when the cellular membrane is damaged. This makes LDH a biomarker for several medical conditions in newborns, such as Ozkiraz-13, necrotizing enterocolitis (NEC), and Asphyxia. EXPERIMENTAL For assembling the slipdisc optically transparent, robust and disposable CD like polycarbonate discs were used with superhydrophobic coating on all except the embedded microfluidic channels. For the LDH assay, heparinized plasma samples were spiked with 7 different concentrations of the LDH enzyme (Lee Biosolutions, USA). These concentrations ranged from clinically normal to abnormal concentrations and used to construct a standard curve for LDH enzyme. RESULTS AND DISCUSSION The ability of the SlipDisc to quantify LDH enzyme levels from plasma samples was evaluated (Fig.3). Using 7 different concentrations, a standard curve with clinically relevant LDH concentrations was obtained (Fig4). Image and data analyses, including linear regression and Pearson’s correlation, were completed using Image processing tool in Matlab. CONCLUSION We demonstrate a low-cost neonatal diagnostics platform for the detection of LDH from plasma using a novel SlipDisc platform. The SlipDisc can further be modified to separate plasma from whole blood samples in order to fully integrate the assay. Its simple operation and smartphone based detection capabilities make it an ideal device for point-of-care neonatal diagnostics. QC 20190819 more...
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- 2016
11. The religious factor in contemporary Europe. Exploring the levels of religious involvement and the relationships between religious involvement, the private, and the public across 23 European countries
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Halman, L.C.J.M. and Petterson, T.
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religion ,ComputingMilieux_LEGALASPECTSOFCOMPUTING - Published
- 1998
12. Globalization and religious change. Structural globalization and new patterns of religious belief systems?
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Halman, L.C.J.M. and Petterson, T.
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globalisation ,religion ,Europe ,ComputingMilieux_LEGALASPECTSOFCOMPUTING - Published
- 1997
13. A north-south divide in basic value profiles? Comparative analyses of values among the mass publics in northern protestant and southern catholic European countries
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Halman, L.C.J.M. and Petterson, T.
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- 1997
14. Morality and religion; A weakened relationship?
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Halman, L.C.J.M., Petterson, T., and TS Social and Behavioral Sciences
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religion ,ComputingMilieux_LEGALASPECTSOFCOMPUTING ,sociology and psychology ,ethics ,moral values - Published
- 1996
15. Well-being and treatment satisfaction in older people with diabetes.
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Petterson, T., Lee, P., Hollis, S., Young, B., Newton, P., Dornan, T. L., Petterson, T., Lee, P., Hollis, S., Young, B., Newton, P., and Dornan, T. L.
- Abstract
OBJECTIVE: To measure well-being and treatment satisfaction and their correlates in older people with diabetes. RESEARCH DESIGN AND METHODS: A postal survey was conducted of 1,000 diabetic patients aged > or = 60 years, representing 56% of the resident older diabetic population in an inner-city health district with a largely indigenous population of 230,000 people and a widely varied socioeconomic mix. Well-being and treatment satisfaction were measured with diabetes-specific instruments and correlated with patient data held in a central register. RESULTS: There was an 81% response. The general well-being scores (median [interquartile range]) for patients on diet alone, tablets, and insulin were 54 (44-60), 53 (42-61), and 48 (35-56) (P < 0.001 comparing insulin with diet and tablets) compared with a scale maximum of 66. Treatment satisfaction scores were 35 (31-36), 35 (32-36), and 34 (30-36) (P < 0.001 comparing insulin with diet and tablets), scale maximum 36. Mean HbA1c concentrations were 5.0 +/- 1.4% (for patients on diet alone), 5.8 +/- 1.6% (tablets), and 6.6 +/- 1.7% (insulin) (P < 0.001 for each difference). Neither well-being nor treatment satisfaction correlated with HbA1c. Insulin-treated patients were younger and had been diabetic longer than non-insulin-treated patients; their well-being remained slightly, but significantly, lower when adjusted for age, sex, BMI, and diabetes duration, but treatment satisfaction was no longer significantly different. Women had lower well-being than men. CONCLUSIONS: It has proved possible to measure well-being and treatment satisfaction in a large community-based samples of older people with diabetes. At the level of glycemic control in this population, neither parameter correlated with HbA1c. The lower well-being in insulin-treated patients remained significant in multivariate analysis. more...
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- 1998
16. Do Older People with Diabetes Feel Well and are They Happy with Their Treatment?
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Petterson, T., primary, Lee, P., additional, Hollis, S., additional, and Dornan, T., additional
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- 1997
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17. Initial endoscopy or empirical therapy with or without testing for Helicobacter pylori for dyspepsia: A decision analysis
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Silverstein, MD, primary, Petterson, T, additional, and Talley, NJ, additional
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- 1996
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18. Prolactin secreting pituitary carcinoma.
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Petterson, T, primary, MacFarlane, I A, additional, MacKenzie, J M, additional, and Shaw, M D, additional
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- 1992
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19. Independent association of high blood pressure and aortic atherosclerosis: A population-based study.
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Agmon, Y, Khandheria, B K, Meissner, I, Schwartz, G L, Petterson, T M, O'Fallon, W M, Gentile, F, Whisnant, J P, Wiebers, D O, and Seward, J B
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- 2000
20. Enabling personal recovery from fibromyalgia - theoretical rationale, content and meaning of a person-centred, recovery-oriented programme.
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Mengshoel AM, Skarbø Å, Hasselknippe E, Petterson T, Brandsar NL, Askmann E, Ildstad R, Løseth L, and Sallinen MH
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- Focus Groups, Humans, Qualitative Research, Stress, Psychological, Fibromyalgia therapy
- Abstract
Background: Fibromyalgia (FM) is a contested, chronic widespread pain syndrome on which recommended therapies have short-lasting, moderate effects. Nevertheless, some patients become symptom-free, and their recovery experiences inspired us to develop a patient-centred recovery-oriented programme (PROP) delivered in a group format. Presently, we describe the theoretical rationale, purpose and content of the PROP, and its meanings for clinicians and patients., Methods: A multidisciplinary clinical team, a leader of a rehabilitation unit, and two researchers coproduced the PROP. Five full-day seminars were arranged to bridge research and clinical experiences. Qualitative studies about patients' illness and recovery experiences and questions by researchers facilitated reflections on clinical experiences. The meaning of the PROP was examined using focus group and individual interviews with patients and clinicians immediately after completing the course and after 1-1.5 years., Results: The biopsychosocial model displays the research evidence across biological, mental and social impacts of FM, justifying that life stress can be an illness-maintaining factor in FM. The content addresses enabling patients to heal their own life and self by modifying life stress. Patients engage in making sense of the relationship between FM, themselves, and life through exploring, discovering and creating appropriate solutions for their daily social life. The PROP reduced uncertainties and brought a positive attitude and hope to the groups. After 1 year, patients are still engaged in recovery work, experience more good days, and maintain hope for further recovery. By sharing and reflecting on clinical experiences, a unified clinical team was established that continues to develop their competency., Conclusion: To our knowledge, the PROP is the first programme for patients with FM that results from a process of coproducing knowledge, is based on explicit theoretical rationale, and facilitates a personal experiential recovery process. PROP is found to be meaningful and to work by patients and clinicians. more...
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- 2021
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21. Parental health in fellowship trainees: Fellows' satisfaction with current policies and interest in innovation.
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Marshall AL, Dines V, Wahner Hendrickson A, Warsame R, Thanarajasingam G, Thompson C, Petterson T, and Wolanskyj-Spinner A
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- Adult, Attitude of Health Personnel, Education, Medical, Graduate, Female, Humans, Male, Middle Aged, Surveys and Questionnaires, Fellowships and Scholarships, Internship and Residency, Parents psychology, Personal Satisfaction, Policy
- Abstract
Background: Parenthood during medical training is common and impacts trainee well-being. However, current graduate medical education parental health policies are often limited in scope. We explored current fellowship trainees' knowledge of/satisfaction with current policies as well as interest in potential changes/additions to existing policies., Methods: Fellowship program directors/coordinators at a three-site academic institution were surveyed and information was collected from 2015 to 2019 regarding fellow demographics and parental health policies. We distributed an electronic survey to fellows containing Likert-type-scale questions rating knowledge/level of satisfaction with current parental health policies and interest in potential additions/modifications to current policies., Results: Thirty-five of 47 (74%) fellowship programs responded. An average of 11% of female fellows and 15% of male fellows took parental leave during the study period. Three (9%) of the programs had at least one additional parental health policy beyond institutional graduate medical education policies. In the fellow survey, 175 of 609 fellows responded (28.7%), of which 84 (48.6%) were female. Although 89.1% agreed/strongly agreed that parental health is an important part of health and well-being for fellows, only 32% were satisfied/very satisfied with current policies (no significant sex-related differences). Fellows reported the following potential interventions as important/very important: 79.2% increased (paid) maternity leave (72.7% male, 86.7% female, p = 0.02), 78% increased (paid) paternity leave (76.4% male, 81.9% female, p = 0.37), 72.3% part-time return to work (60.2% male, 84.3% female, p = 0.0005), 63% coverage for workup/management of infertility (52.3% male, 74.7% female, p = 0.002), and 79.9% on-site day care (70.7% male, 89.2% female, p = 0.003)., Conclusions: Parental health includes multiple domains, not all of which are covered by current policies. Fellows feel that parental health is an important part of overall health and well-being, but most are not satisfied with current policies. Expanded access to parental leave and new policies (part-time return to work, infertility management, and on-site day care) are opportunities for innovation. more...
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- 2020
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22. Incidence of Adenovirus Infection in Hematopoietic Stem Cell Transplantation Recipients: Findings from the AdVance Study.
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Sedláček P, Petterson T, Robin M, Sivaprakasam P, Vainorius E, Brundage T, Chandak A, Mozaffari E, Nichols G, and Voigt S
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- Adenoviridae Infections pathology, Adolescent, Adult, Aged, Child, Child, Preschool, Female, Humans, Incidence, Infant, Male, Middle Aged, Young Adult, Adenoviridae Infections etiology, Hematopoietic Stem Cell Transplantation adverse effects, Transplantation Conditioning adverse effects
- Abstract
Adenovirus (AdV) is an increasingly recognized threat to recipients of allogeneic hematopoietic stem cell transplantation (allo-HCT), particularly when infection is prolonged and unresolved. AdVance is the first multinational, multicenter study to evaluate the incidence of AdV infection in both pediatric and adult allo-HCT recipients across European transplantation centers. Medical records for patients undergoing first allo-HCT between January 2013 and September 2015 at 50 participating centers were reviewed. The cumulative incidence of AdV infection (in any sample using any assay) during the 6 months after allo-HCT was 32% (95% confidence interval [CI], 30.9% to 33.4%) among pediatric allo-HCT recipients (n = 1736) and 6% (95% CI, 4.7% to 6.4%) among adult allo-HCT recipients (n = 2540). The incidence of AdV viremia ≥1000copies/mL (a common threshold for initiation of preemptive treatment) was 14% (95% CI, 13.0% to 14.8%) in pediatric recipients and 1.5% (95% CI, 1.1% to 2.0%) in adult recipients. Baseline risk factors for developing AdV viremia ≥1000copies/mL included younger age, use of T cell depletion, and donor type other than matched related. Baseline demographic factors were broadly comparable across patients of all ages and identified by multivariate analyses. Notably, the incidence of AdV infection decreased stepwise with increasing age; younger adults (age 18 to 34 years) had a similar incidence as older pediatric patients (<18 years). This study provides a contemporary multicenter understanding of the incidence and risk factors for AdV infection following allo-HCT. Our findings may help optimize infection screening and intervention criteria, particularly for younger at-risk adults., (Copyright © 2018 American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.) more...
- Published
- 2019
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23. Brincidofovir is highly efficacious in controlling adenoviremia in pediatric recipients of hematopoietic cell transplant.
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Hiwarkar P, Amrolia P, Sivaprakasam P, Lum SH, Doss H, O'Rafferty C, Petterson T, Patrick K, Silva J, Slatter M, Lawson S, Rao K, Steward C, Gassas A, Veys P, and Wynn R
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- Adenoviridae Infections etiology, Adolescent, Allografts, Child, Child, Preschool, Cytosine administration & dosage, Cytosine adverse effects, Female, Humans, Male, Organophosphonates adverse effects, Viremia etiology, Adenoviridae, Adenoviridae Infections drug therapy, Cytosine analogs & derivatives, Hematopoietic Stem Cell Transplantation, Organophosphonates administration & dosage, Viremia drug therapy
- Abstract
Cidofovir is preemptively used for controlling adenoviremia and preventing disseminated viral disease in hematopoietic cell transplant (HCT) recipients but does not lead to resolution of viremia without T-cell immune-reconstitution. The lipid-conjugated prodrug of cidofovir, brincidofovir, has improved oral bioavailability and achieves higher intracellular concentrations of active drug. We present retrospective multicenter data comparing the kinetics of viremia and toxicities following preemptive treatment with and brincidofovir in children and adolescents diagnosed with HCT-related adenoviremia. Forty-one episodes (18 = brincidofovir; 23 = cidofovir) of antiviral therapy were observed in 27 patients. The 2 groups had comparable immune-reconstitution and viral burden. Major (≥2 log-reduction in 2 weeks; n = 13) and minor (≥1 to ≤2 log-reduction in 2 weeks; n = 2) virological responses were observed in 15 (83%) brincidofovir episodes compared to only 2 (9%) major virological responses with cidofovir ( P < .0001). Brincidofovir mediated major responses in 9 of 11 cidofovir-unresponsive patients and resulted in complete responses (CR) despite significant lymphopenia (Brincidofovir vs cidofovir; CR = 13 (80%) vs 8 (35%); median lymphocyte count = 320/μl vs 910/μl; P < .05). One patient experienced abdominal cramps and diarrhea necessitating interruption of brincidofovir and none developed nephrotoxicity with brincidofovir. Thus, brincidofovir is well-tolerated and highly efficacious in controlling adenoviremia during the lymphopenic phase of HCT., (© 2017 by The American Society of Hematology.) more...
- Published
- 2017
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24. Yrjö Tapio Konttinen 1952-2014.
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Pajarinen J, Nordström D, Petterson T, Ainola M, Gómez-Barrena E, Takagi M, and Goodman SB
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- History, 20th Century, History, 21st Century, Sweden, Rheumatology history
- Published
- 2015
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25. Hypothermic overdose, not all bad?
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Petterson T, Lyon L, and Peckler B
- Abstract
A 51-year-old woman was brought into the Emergency Department (ED) following an intentional overdose of alcohol and her medication. Along with two bottles of wine it was estimated that she had taken 5800 mg of Quetiapine and 240 mg of Citalopram along with the wine. The ambient temperature in her flat was thought to be 10°C. On arrival to the ED her GCS was 8. She had agonal respirations with a pulse of 56/min, hypotensive 55/35 mmHg and a temperature 24°C. The patient was intubated and was given sodium bicarbonate, magnesium sulphate, calcium gluconate and an adrenaline infusion. She received active and passive rewarming measures. She had significant ECG findings related to her hypothermia and polypharmacy overdose which seemed to have been cumulative. The patient recovered and the only neurological deficit was numbness in her left leg which was thought to be related to prolonged immobility. Hypothermia may have contributed to her good outcome as hypothermia has been shown to improve both cardiac and neurological outcome. more...
- Published
- 2013
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26. NOD-like receptors and RIG-I-like receptors in human eosinophils: activation by NOD1 and NOD2 agonists.
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Kvarnhammar AM, Petterson T, and Cardell LO
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- Acetylmuramyl-Alanyl-Isoglutamine pharmacology, Antigens, CD metabolism, Antigens, Differentiation, T-Lymphocyte metabolism, CD11b Antigen metabolism, Carrier Proteins genetics, Cell Movement drug effects, Cells, Cultured, DEAD Box Protein 58, DEAD-box RNA Helicases genetics, Diaminopimelic Acid analogs & derivatives, Diaminopimelic Acid pharmacology, Eosinophil-Derived Neurotoxin metabolism, Eosinophils cytology, Flow Cytometry, Gene Expression drug effects, Humans, Immunohistochemistry, Interferon-Induced Helicase, IFIH1, Interleukin-8 metabolism, L-Selectin metabolism, Lectins, C-Type metabolism, NF-kappa B metabolism, NLR Family, Pyrin Domain-Containing 3 Protein, Nod1 Signaling Adaptor Protein agonists, Nod1 Signaling Adaptor Protein genetics, Nod1 Signaling Adaptor Protein metabolism, Nod2 Signaling Adaptor Protein agonists, Nod2 Signaling Adaptor Protein genetics, Nod2 Signaling Adaptor Protein metabolism, Receptors, Immunologic, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction drug effects, Carrier Proteins metabolism, DEAD-box RNA Helicases metabolism, Eosinophils metabolism
- Abstract
NOD-like receptors (NLRs) and RIG-I-like receptors (RLRs) are newly discovered pattern-recognition receptors. They detect substructures of bacterial peptidoglycan and viral RNA, respectively, thereby initiating an immune response. However, their role in eosinophil activation remains to be explored. The aim of this study was to characterize the expression of a range of NLRs and RLRs in purified human eosinophils and assess their functional importance. Expression of NOD1, NOD2, NLRP3, RIG-I and MDA-5 was investigated using real-time reverse transcription PCR, flow cytometry and immunohistochemistry. The effects of the corresponding agonists iE-DAP (NOD1), MDP (NOD2), alum (NLRP3) and poly(I:C)/LyoVec (RIG-I/MDA-5) were studied in terms of cytokine secretion, degranulation, survival, expression of adhesion molecules and activation markers, and chemotactic migration. Eosinophils expressed NOD1 and NOD2 mRNA and protein. Low levels of RIG-I and MDA-5 were found, whereas expression of NLRP3 was completely absent. In accordance, stimulation with iE-DAP and MDP was found to induce secretion of interleukin-8, up-regulate expression of CD11b, conversely down-regulate CD62 ligand, increase expression of CD69 and induce migration. The MDP also promoted release of eosinophil-derived neurotoxin, whereas iE-DAP failed to do so. No effects were seen upon stimulation with alum or poly(I:C)/LyoVec. Moreover, the NOD1-induced and NOD2-induced activation was mediated via the nuclear factor-κB signalling pathway and augmented by interleukin-5 and granulocyte-macrophage colony-stimulating factor, but not interferon-γ. Taken together, the NLR system represents a novel pathway for eosinophil activation. The responses are enhanced in the presence of cytokines that regulate T helper type 2 immunity, suggesting that the NLRs constitute a link between respiratory infections and exacerbations of allergic disease., (© 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd.) more...
- Published
- 2011
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27. Nucleotide-binding and oligomerization domain-like receptors and retinoic acid inducible gene-like receptors in human tonsillar T lymphocytes.
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Petterson T, Månsson A, Riesbeck K, and Cardell LO
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- Carrier Proteins immunology, Cell Proliferation, Cell Separation, DEAD-box RNA Helicases immunology, Flow Cytometry, Gene Expression Profiling, Humans, Immunohistochemistry, Interferon-Induced Helicase, IFIH1, NLR Family, Pyrin Domain-Containing 3 Protein, Nod1 Signaling Adaptor Protein immunology, Nod2 Signaling Adaptor Protein immunology, Palatine Tonsil cytology, Palatine Tonsil immunology, Palatine Tonsil metabolism, RNA, Messenger analysis, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes immunology, Carrier Proteins biosynthesis, DEAD-box RNA Helicases biosynthesis, Lymphocyte Activation immunology, Nod1 Signaling Adaptor Protein biosynthesis, Nod2 Signaling Adaptor Protein biosynthesis, T-Lymphocytes metabolism
- Abstract
Nucleotide-binding and oligomerization domain (NOD)-like receptors (NLRs) and retinoic acid-inducible gene (RIG)-like receptors (RLRs) are recently discovered cytosolic pattern-recognition receptors sensing mainly bacterial components and viral RNA, respectively. Their importance in various cells and disorders is becoming better understood, but their role in human tonsil-derived T lymphocytes remains to be elucidated. In this study, we evaluated expression and functional relevance of NLRs and RLRs in human tonsillar CD3(+) T lymphocytes. Immunohistochemistry, real-time RT-PCR and flow cytometry revealed expression of NOD1, NOD2, NALP1, NALP3, NAIP, IPAF, RIG-1, MDA-5 and LGP-2 at mRNA and protein levels. Because of the limited number of ligands (iE-DAP, MDP, Alum, Poly(I:C)/LyoVec), functional evaluation was restricted to NOD1, NOD2, NALP3 and RIG-1/MDA-5, respectively. Stimulation with the agonists alone was not enough to induce activation but upon triggering via CD3 and CD28, a profound induction of proliferation was seen in purified CD3(+) T cells. However, the proliferative response was not further enhanced by the cognate ligands. Nonetheless, in tonsillar mononuclear cells iE-DAP, MDP and Poly(I:C)/LyoVec were found to augment the CD3/CD28-induced proliferation of tonsillar mononuclear cells. Also, iE-DAP and MDP were found to promote secretion of interleukins 2 and 10 as well as to up-regulate CD69. This study demonstrates for the first time a broad range of NLRs and RLRs in human tonsillar T cells and that NOD1, NOD2 and RIG-1/MDA-5 act synergistically with αCD3 and αCD28 to induce proliferation of human T cells. Hence, these results suggest that these receptors have a role in T-cell activation., (© 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd.) more...
- Published
- 2011
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28. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors.
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Petterson T, Jendholm J, Månsson A, Bjartell A, Riesbeck K, and Cardell LO
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- Adolescent, B-Lymphocytes metabolism, Cells, Cultured, Child, Child, Preschool, Humans, Lymphocyte Activation drug effects, Lymphocyte Activation immunology, Nod1 Signaling Adaptor Protein agonists, Nod1 Signaling Adaptor Protein genetics, Nod2 Signaling Adaptor Protein agonists, Nod2 Signaling Adaptor Protein genetics, B-Lymphocytes immunology, Nod1 Signaling Adaptor Protein metabolism, Nod2 Signaling Adaptor Protein metabolism, Receptor Cross-Talk immunology, Toll-Like Receptors physiology
- Abstract
NLRs are recently discovered PRRs detecting substructures of peptidoglycans and triggering innate immunity. NLRs are expressed in several cell types, but the presence in human B lymphocytes is still unknown. This study aimed to investigate expression and function of NLRs in human B lymphocytes. B cells were isolated and analyzed for mRNA and protein expression. The functional responsiveness of NOD1 and NOD2 was investigated upon stimulation with the cognate ligands, with or without stimulation via IgM/IgD/CD40 and/or selected TLR agonists. A differential expression of NLRs was demonstrated in blood-derived and tonsillar B cells, whereas no variations were found among naive, germinal center, or memory B cells. Stimulation with the ligands alone did not induce B cell activation. However, upon concomitant BCR triggering, an increase in proliferation was seen, together with an induction of cell surface markers (CD27, CD69, CD71, CD80, CD86, and CD95) and prolonged survival. Peripheral B cells were activated by NOD1 and NOD2 ligands, whereas tonsil-derived B cells responded solely to NOD1. In contrast, costimulation with CD40L failed to induce activation. Additionally, it was found that NLR ligands could enhance TLR-induced proliferation of B cells. The present study demonstrates expression of functional NLRs in human B cells. We show that NOD1 and NOD2 have the ability to augment the BCR-induced activation independently of physical T cell help. Hence, NLRs represent a new pathway for B cell activation and a potentially important host defense system against bacterial infections. more...
- Published
- 2011
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29. Incidence of venous thromboembolism in hospitalized patients vs community residents.
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Heit JA, Melton LJ 3rd, Lohse CM, Petterson TM, Silverstein MD, Mohr DN, and O'Fallon WM
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- Adolescent, Adult, Age Distribution, Aged, Aged, 80 and over, Child, Child, Preschool, Confidence Intervals, Female, Humans, Incidence, Infant, Male, Middle Aged, Minnesota epidemiology, Retrospective Studies, Sex Distribution, Community-Acquired Infections epidemiology, Hospitalization, Pulmonary Embolism epidemiology, Venous Thrombosis epidemiology
- Abstract
Objective: To estimate the incidence rates of deep venous thrombosis (DVT) and pulmonary embolism (PE) in hospitalized patients and to compare these with incidence rates in community residents., Patients and Methods: We performed a retrospective review of the complete medical records from a population-based inception cohort of patients who resided in Olmsted County, Minnesota, and had an incident DVT or PE from 1980 through 1990., Results: From 1980 through 1990, 911 Olmsted County residents experienced their first lifetime event of definite, probable, or possible venous thromboembolism. Of these residents, 253 had been hospitalized for some reason other than a diagnosis of DVT or PE (in-hospital cases), and 658 were not hospitalized at onset of venous thromboembolism (community residents). The average annual age- and sex-adjusted incidence of in-hospital venous thromboembolism was 960.5 (95% confidence interval, 795.1-1125.9) per 10,000 person-years and was more than 100 times greater than the incidence among community residents at 7.1 (95% confidence interval, 6.5-7.6) per 10,000 person-years. The incidence of venous thromboembolism rose markedly with increasing age for both groups, with PE accounting for most of the age-related increase among in-hospital cases. Incidence rates in the 2 groups changed little over time despite a reduction in the average length of hospital stay between 1980 and 1990., Conclusions: Venous thromboembolism is a major national health problem, especially among elderly hospitalized patients. This finding emphasizes the need for accurate identification of hospitalized patients at risk for venous thromboembolism and a better understanding of the mechanisms involved so that safe and effective prophylaxis can be implemented. more...
- Published
- 2001
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30. Extent of high-grade dysplasia in Barrett's esophagus correlates with risk of adenocarcinoma.
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Buttar NS, Wang KK, Sebo TJ, Riehle DM, Krishnadath KK, Lutzke LS, Anderson MA, Petterson TM, and Burgart LJ
- Subjects
- Adult, Aged, Barrett Esophagus complications, Female, Follow-Up Studies, Humans, Male, Middle Aged, Risk, Adenocarcinoma etiology, Barrett Esophagus pathology, Esophageal Neoplasms etiology, Esophagus pathology
- Abstract
Background & Aims: The identification of any high-grade dysplasia (HGD) in Barrett's esophagus has been considered to be an indication for esophagectomy because of the increased risk of cancer. The aim of this study was to determine if a limited extent of HGD has the same potential for cancer as diffuse HGD., Methods: A retrospective cohort study was performed to assess the risk of developing adenocarcinoma in relationship to the extent of HGD found on endoscopic surveillance. The extent of HGD was defined as focal if cytologic and/or architectural changes of HGD were limited to a single focus of 5 or fewer crypts and diffuse if more than 5 crypts were involved in a single biopsy specimen or if HGD involved more than one biopsy fragment. The relative risk of cancer was assessed using a Cox proportional hazard model, and cancer-free survival was determined using survival curves., Results: Sixty-seven patients with diffuse HGD and 33 with focal HGD satisfied selection criteria. Cancer-free survival rates at 1 and 3 years were 93% and 86% for focal HGD compared with 62% and 44% for diffuse HGD (P < 0.001). On univariate analysis, extent of HGD (relative risk, 5.36; 95% confidence interval, 1.84-15.56), nodularity on endoscopy (relative risk, 3.98; 95% confidence interval, 1.97-8.04), and lack of acid suppression (relative risk, 2.48; 95% confidence interval, 1.16-5.28) were associated with an increased risk of esophageal adenocarcinoma. Diffuse HGD had a 3.7-fold increase in the risk of esophageal cancer compared with focal HGD (P = 0.02) on multivariate analysis., Conclusions: Patients with focal HGD are less likely to have cancer during the first year after diagnosis or on subsequent follow-up compared with diffuse HGD. more...
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- 2001
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31. Trends in the incidence of venous stasis syndrome and venous ulcer: a 25-year population-based study.
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Heit JA, Rooke TW, Silverstein MD, Mohr DN, Lohse CM, Petterson TM, O'Fallon WM, and Melton LJ 3rd
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- Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Incidence, Male, Middle Aged, Minnesota epidemiology, Retrospective Studies, Varicose Ulcer epidemiology, Venous Insufficiency epidemiology
- Abstract
Background: The incidence rates of venous stasis syndrome and venous ulcer are uncertain, and trends in incidence are unknown., Methods: We performed a retrospective review of the complete (inpatient and outpatient) medical records of a community population (Olmsted County, Minnesota) to estimate the incidence of venous stasis syndrome and venous ulcer during the 25-year period, 1966 to 1990, and to describe trends in incidence., Results: A total of 1131 patients received a first lifetime diagnosis of venous stasis syndrome. A total of 263 patients received a first lifetime diagnosis of venous ulcer. The overall incidence of venous stasis syndrome and venous ulcer were 76.1 and 18.0 per 100,000 person-years, respectively. The incidence of both was higher in women than in men (83.7 vs 67.4 per 100,000 person-years for venous stasis syndrome; 20.4 vs 14.6 per 100,000 for venous ulcer) and increased with age for both sexes. There was no clear trend in the incidence of venous stasis syndrome over the 25-year period. Compared with 1966 to 1970, the incidence of venous ulcer decreased in 1971 to 1980, but was unchanged after 1981. Among 945 patients with venous stasis only, 60 subsequently had a venous ulcer. The average (+/- SD) time from venous stasis diagnosis to development of a venous ulcer was 5.0 (+/- 5.0) years., Conclusion: Venous stasis syndrome and venous ulcer are common, especially in the elderly population. The incidence of venous stasis syndrome has not changed since 1966, and venous ulcer incidence is unchanged since 1981. More accurate identification of patients at risk for venous stasis syndrome and venous ulcer and more effective prevention are needed. more...
- Published
- 2001
- Full Text
- View/download PDF
32. Association of atrial fibrillation and aortic atherosclerosis: a population-based study.
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Agmon Y, Khandheria BK, Meissner I, Schwartz GL, Petterson TM, O'Fallon WM, Gentile F, Spittell PC, Whisnant JP, Wiebers DO, Covalt JL, and Seward JB
- Subjects
- Adult, Age Distribution, Aged, Aortic Diseases diagnostic imaging, Arteriosclerosis diagnostic imaging, Carotid Arteries diagnostic imaging, Echocardiography, Echocardiography, Transesophageal, Female, Humans, Hypertension complications, Male, Middle Aged, Random Allocation, Risk Factors, Sex Distribution, Aortic Diseases complications, Arteriosclerosis complications, Atrial Fibrillation complications
- Abstract
Objective: To examine the association between atrial fibrillation (AF) and aortic atherosclerosis in the general population., Subjects and Methods: Transesophageal echocardiography was performed in 581 subjects, a random sample of the adult Olmsted County, Minnesota, population (45 years of age or older) participating in the Stroke Prevention: Assessment of Risk in a Community (SPARC) study. The frequency of aortic atherosclerosis was determined in 42 subjects with AF and compared with that in 539 subjects without AF (non-AF group)., Results: Subjects with AF were significantly older than non-AF subjects (mean +/- SD age, 82+/-10 vs 66+/-13 years, respectively; P<.001) and more commonly had hypertension (28 [66.7%] vs 288 [53.4%], respectively; P=.10). The 2 groups were similar in sex and frequency of diabetes mellitus, hyperlipidemia, or smoking history (P>.10). The odds of aortic atherosclerosis (of any degree) were 2.87 times greater (95% confidence interval [CI], 1.41-5.83; P=.004) and the odds of complex atherosclerosis (protruding atheroma >4 mm thick, mobile debris, or plaque ulceration) were 2.71 times greater (CI, 1.13-6.53; P=.03) in the AF group than in the non-AF group. Age was a significant predictor of aortic atherosclerosis (P<.001). After adjusting for age, the odds of atherosclerosis and complex atherosclerosis were not significantly different between the 2 groups (P=.13 and P=.75, respectively)., Conclusions: In the general population, AF is associated with aortic atherosclerosis, including complex atherosclerosis. This association is related to age since both AF and aortic atherosclerosis are more frequent in the elderly population. more...
- Published
- 2001
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33. The venous stasis syndrome after deep venous thrombosis or pulmonary embolism: a population-based study.
- Author
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Mohr DN, Silverstein MD, Heit JA, Petterson TM, O'Fallon WM, and Melton LJ
- Subjects
- Adult, Aged, Cohort Studies, Disease-Free Survival, Female, Humans, Incidence, Male, Middle Aged, Minnesota epidemiology, Multivariate Analysis, Proportional Hazards Models, Retrospective Studies, Risk Factors, Varicose Ulcer epidemiology, Varicose Ulcer mortality, Venous Insufficiency epidemiology, Venous Insufficiency mortality, Pulmonary Embolism complications, Varicose Ulcer etiology, Venous Insufficiency etiology, Venous Thrombosis complications
- Abstract
Objectives: To estimate the incidence and determine predictors of venous stasis syndrome and venous ulcers after deep venous thrombosis and pulmonary embolism., Patients and Methods: This population-based retrospective cohort study reviewed medical records of 1527 patients with incident deep venous thrombosis or pulmonary embolism between 1966 and 1990. We recorded baseline characteristics, event type (deep venous thrombosis with or without pulmonary embolism or pulmonary embolism alone), leg side and site of deep venous thrombosis (proximal with or without distal deep venous thrombosis vs distal deep venous thrombosis alone), and venous stasis syndrome and venous ulcer., Results: Two hundred forty-five patients developed venous stasis syndrome. One-year, 5-year, 10-year, and 20-year cumulative incidence rates were 7.3%, 14.3%, 19.7%, and 26.8%, respectively. By 20 years the cumulative incidence of venous ulcers was 3.7%. Patients with deep venous thrombosis with or without pulmonary embolism were 2.4-fold (95% confidence interval, 1.7-fold-3.2-fold) more likely to develop venous stasis syndrome than patients with pulmonary embolism and no diagnosed deep venous thrombosis. In patients aged 40 years or younger with proximal compared with distal-only deep venous thrombosis, venous stasis syndrome was 3.0-fold more likely (95% confidence interval, 1.6-fold-4.7-fold). In patients with unilateral leg deep venous thrombosis, venous stasis syndrome usually developed in the concordant leg (P < .001). There was a 30% (95% confidence interval, 2%-62%) increased risk for venous ulcer per decade of age at the incident venous thromboembolism., Conclusions: The cumulative incidence of venous stasis syndrome continues to increase for 20 years after venous thromboembolism. Pulmonary embolism alone is less likely to cause venous stasis syndrome. more...
- Published
- 2000
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34. Graft-related complications after abdominal aortic aneurysm repair: reassurance from a 36-year population-based experience.
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Hallett JW Jr, Marshall DM, Petterson TM, Gray DT, Bower TC, Cherry KJ Jr, Gloviczki P, and Pairolero PC
- Subjects
- Adult, Aged, Aged, 80 and over, Aneurysm, False etiology, Aortic Aneurysm, Abdominal mortality, Blood Vessel Prosthesis mortality, Colon blood supply, Female, Fistula etiology, Follow-Up Studies, Graft Occlusion, Vascular, Hemorrhage etiology, Humans, Intestinal Fistula etiology, Ischemia etiology, Male, Middle Aged, Minnesota epidemiology, Prosthesis-Related Infections, Survival Rate, Thrombosis etiology, United States epidemiology, Aortic Aneurysm, Abdominal surgery, Blood Vessel Prosthesis adverse effects
- Abstract
Purpose: Graft-related complications must be factored into the long-term morbidity and mortality rates of abdominal aortic aneurysm (AAA) repair. However, the true incidence may be underestimated because some patients do not return to the original surgical center when a problem arises., Methods: To minimize referral bias and loss to follow-up, we studied all patients who underwent AAA repair between 1957 and 1990 in a geographically defined community where all AAA operations were performed and followed by a single surgical practice. All patients who remained alive were asked to have their aortic grafts imaged., Results: Among 307 patients who underwent AAA repair, 29 patients (9.4%) had a graft-related complication. At a mean follow-up of 5.8 years (range, < 30 days to 36 years), the most common complication was anastomotic pseudoaneurysm (3.0%), followed by graft thrombosis (2.0%), graft-enteric erosion/fistula (1.6%), graft infection (1.3%), anastomotic hemorrhage (1.3%), colon ischemia (0.7%), and atheroembolism (0.3%). Complications were recognized within 30 days after surgery in eight patients (2.6%) and at late follow-up in 21 patients (6.8%). These complications were observed at a median follow-up of 6.1 years for anastomotic pseudoaneurysm, 4.3 years for graft-enteric erosion, and 0.15 years for graft infection. Kaplan-Meier 5- and 10-year survival free estimates were 98% and 96% for anastomotic pseudoaneurysm, 98% and 95% for combined graft-enteric erosion/infection, and 98% and 97% for graft thrombosis., Conclusions: This 36-year population-based study confirms that the vast majority of patients who undergo standard surgical repair of an abdominal aortic aneurysm remain free of any significant graft-related complication during their remaining lifetime. more...
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- 1997
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35. Bleeding and thromboembolism during anticoagulant therapy: a population-based study in Rochester, Minnesota.
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Gitter MJ, Jaeger TM, Petterson TM, Gersh BJ, and Silverstein MD
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- Age Factors, Aged, Female, Hemorrhage chemically induced, Humans, Incidence, Male, Middle Aged, Minnesota epidemiology, Multivariate Analysis, Peptic Ulcer complications, Population Surveillance, Predictive Value of Tests, Retrospective Studies, Risk Factors, Severity of Illness Index, Thromboembolism chemically induced, Anticoagulants adverse effects, Hemorrhage epidemiology, Hemorrhage etiology, Neoplasms complications, Thromboembolism epidemiology, Thromboembolism etiology
- Abstract
Objective: To estimate the incidence of and identify risk factors for hemorrhage and thromboembolism during long-term anticoagulant therapy., Design: We conducted a population-based retrospective cohort study of all residents of Rochester, Minnesota, in whom a course of warfarin therapy intended to last for more than 4 weeks was initiated between Sept. 1, 1987, and Dec. 31, 1989., Methods: Medical records were reviewed, and pertinent data were compiled. All bleeding complications were classified as minor or major on the basis of the bleeding severity index, and thromboembolic events were classified as major if they were fatal or life-threatening. Cumulative incidences of adverse events were analyzed statistically., Results: During the study period, 261 patients had incident courses of anticoagulation (52% were male, 61% were 65 years of age or older, and 31% were 75 years of age or older), with 221 patient-years of warfarin exposure. The primary indications for anticoagulation were venous thromboembolism (39%); stroke or transient ischemic attack (21%); atrial fibrillation (11%); and coronary artery disease, procedures for coronary artery disease, or cardiomyopathy (7%). The cumulative incidence of major hemorrhage at 1, 3, 12, and 24 months was 1.6%, 3.3%, 5.3%, and 10.6%, respectively, and of major or minor thromboembolic events was 2.3%, 5.0%, 7.4%, and 13.1%, respectively. In multivariate analysis, (1) a malignant condition was significantly associated with major hemorrhage; (2) malignant disease and history of peptic ulcer were significantly associated with the combined outcome of major or minor hemorrhage; and (3) malignant disease was significantly associated with any thromboembolism. Age, sex, atrial fibrillation, history of gastrointestinal hemorrhage, history of peptic ulcer, alcohol abuse, hypertension, stroke, and the Charlson comorbidity index were not significantly associated with major hemorrhage., Conclusion: In this population-based study, including a high proportion of elderly patients, malignant disease at initiation of warfarin anticoagulation was significantly associated with both major hemorrhage and any thromboembolism. Advanced age is not a contraindication to anticoagulant therapy. more...
- Published
- 1995
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36. Mesenteric venous thrombosis: still a lethal disease in the 1990s.
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Rhee RY, Gloviczki P, Mendonca CT, Petterson TM, Serry RD, Sarr MG, Johnson CM, Bower TC, Hallett JW Jr, and Cherry KJ Jr
- Subjects
- Acute Disease, Adult, Aged, Aged, 80 and over, Algorithms, Anticoagulants therapeutic use, Chronic Disease, Combined Modality Therapy, Female, Humans, Male, Mesenteric Veins diagnostic imaging, Middle Aged, Postoperative Care, Postoperative Complications mortality, Reoperation, Retrospective Studies, Sensitivity and Specificity, Survival Rate, Time Factors, Tomography, X-Ray Computed, Treatment Outcome, Vascular Surgical Procedures methods, Mesenteric Vascular Occlusion diagnosis, Mesenteric Vascular Occlusion etiology, Mesenteric Vascular Occlusion mortality, Mesenteric Vascular Occlusion therapy, Thrombosis diagnosis, Thrombosis etiology, Thrombosis mortality, Thrombosis therapy
- Abstract
Purpose: This study was designed to evaluate progress in diagnosis, management, and clinical outcome of mesenteric venous thrombosis (MVT)., Methods: We retrospectively reviewed the clinical course of 72 patients treated for mesenteric venous thrombosis between 1972 and 1993., Results: Fifty-three patients had acute and 19 had chronic mesenteric venous thrombosis. Fifty-seven patients had secondary mesenteric venous thrombosis; previous abdominal surgical procedure and hypercoagulable states were the most prevalent associated conditions. Computed tomography was abnormal in all patients who underwent this test for acute mesenteric venous thrombosis and in 93% of those who had chronic disease. Angiography diagnosed acute mesenteric venous thrombosis in five (72%) of seven patients. Acute mesenteric venous thrombosis presented most frequently as abdominal pain (83%), anorexia (53%), and diarrhea (43%). Thirty-three (75%) had symptoms longer than 48 hours. Thirty-four (64%) patients with acute mesenteric venous thrombosis underwent a surgical procedure. Bowel resection was necessary in 31 patients. One patient had unsuccessful mesenteric venous thrombectomy. Seven patients with acute mesenteric venous thrombosis underwent anticoagulation without a surgical procedure, and 12 were observed. All patients with chronic mesenteric venous thrombosis were observed; nine of the 19 underwent anticoagulation. The median delay in diagnosis for patients with acute mesenteric venous thrombosis was 48 hours and did not decrease during the last decade. Mesenteric venous thrombosis recurred in 19 (36%) patients. The 30-day mortality was 27%. Long-term survival of patients with acute mesenteric venous thrombosis was significantly worse than that of those with chronic disease (36% vs 83% survival at 3 years). The patients with acute mesenteric venous thrombosis who underwent anticoagulation with and without surgical procedure had improved survival when compared with the observed group., Conclusion: Acute mesenteric venous thrombosis remains a lethal disease. Mortality has not improved in the last 22 years. Computed tomography is the most sensitive diagnostic test. Anticoagulation and surgical procedure enhanced survival in the acute subgroup. The underlying disease determined survival in chronic disease. more...
- Published
- 1994
- Full Text
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37. Pleural fluid lysozyme in tuberculous and non-tuberculous pleurisy.
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Klockars M, Petterson T, Riska H, and Hellström PE
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- Humans, Muramidase blood, Pleural Effusion enzymology, Muramidase analysis, Pleurisy enzymology, Tuberculosis, Pleural enzymology
- Published
- 1976
- Full Text
- View/download PDF
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