10 results on '"Peng‐Fei, Luo"'
Search Results
2. Targeted release of stromal cell-derived factor-1α by reactive oxygen species-sensitive nanoparticles results in bone marrow stromal cell chemotaxis and homing, and repair of vascular injury caused by electrical burns.
- Author
-
Fang He, Peng-Fei Luo, Tao Tang, Fang Zhang, He Fang, Shi-Zhao Ji, Yu Sun, Guo-Sheng Wu, Bo-Han Pan, Zhi-Bao Huo, Guang-Yi Wang, and Zhao-Fan Xia
- Subjects
Medicine ,Science - Abstract
Rapid repair of vascular injury is an important prognostic factor for electrical burns. This repair is achieved mainly via stromal cell-derived factor (SDF)-1α promoting the mobilization, chemotaxis, homing, and targeted differentiation of bone marrow mesenchymal stem cells (BMSCs) into endothelial cells. Forming a concentration gradient from the site of local damage in the circulation is essential to the role of SDF-1α. In a previous study, we developed reactive oxygen species (ROS)-sensitive PPADT nanoparticles containing SDF-1α that could degrade in response to high concentration of ROS in tissue lesions, achieving the goal of targeted SDF-1α release. In the current study, a rat vascular injury model of electrical burns was used to evaluate the effects of targeted release of SDF-1α using PPADT nanoparticles on the chemotaxis of BMSCs and the repair of vascular injury. Continuous exposure to 220 V for 6 s could damage rat vascular endothelial cells, strip off the inner layer, significantly elevate the local level of ROS, and decrease the level of SDF-1α. After injection of Cy5-labeled SDF-1α-PPADT nanoparticles, the distribution of Cy5 fluorescence suggested that SDF-1α was distributed primarily at the injury site, and the local SDF-1α levels increased significantly. Seven days after injury with nanoparticles injection, aggregation of exogenous green fluorescent protein-labeled BMSCs at the injury site was observed. Ten days after injury, the endothelial cell arrangement was better organized and continuous, with relatively intact vascular morphology and more blood vessels. These results showed that SDF-1α-PPADT nanoparticles targeted the SDF-1α release at the site of injury, directing BMSC chemotaxis and homing, thereby promoting vascular repair in response to electrical burns.
- Published
- 2018
- Full Text
- View/download PDF
3. Photomechanical polymer hydrogels based on molecular photoswitches
- Author
-
Shi-Li Xiang, Chong Li, Peng-Fei Luo, and Ming-Qiang Zhu
- Subjects
chemistry.chemical_classification ,Photochromism ,Materials science ,Polymers and Plastics ,chemistry ,Self-healing hydrogels ,Materials Chemistry ,Nanotechnology ,Artificial muscle ,Polymer ,Physical and Theoretical Chemistry - Published
- 2021
4. Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects
- Author
-
Yun-Qing Zhang, Shi-Zhao Ji, He Fang, Yong-Jun Zheng, Peng-Fei Luo, Hai-Bin Wu, Min-Juan Wu, Zhi-Hong Wang, Shi-Chu Xiao, and Zhao-Fan Xia
- Subjects
Medicine - Abstract
As angiogenesis and vasculogenesis involve the complex network structures of various types of cells, extracellular matrix components, and cytokines, it is still difficult to exactly mimic the microenvironment of vascularization in vivo. In our study, we constructed a complex containing highly proliferative fibroblasts that can secrete extracellular matrix components and growth factors to chemotaxize endothelial progenitor cells (EPCs) in an attempt to create an ideal microenvironment for quick vascularization. Amniotic membrane microparticles (mAM) rich in type IV collagen (COL IV) and laminin (LN) were prepared, and human dermal fibroblasts (HDF) were infected with lentivirus (LV) of overexpression of SDF-1α to construct SDF-1α ov HDF. Using the rotary cell culture system (RCCS), mAM was loaded with HDF or SDF-1α ov HDF to construct HDF-mAM and SDF-1α ov HDF-mAM complexes. The complexes were able to secrete various types of active peptides (IL-6, IL-8, TGF-β, and bFGF) during in vitro culture. In addition, SDF-1α ov HDF-mAM complex highly expressed SDF-1α. Transwell assay showed SDF-1α ov HDF-mAM complex had an apparent chemotactic effect on EPCs. Transplantation of complexes onto full-thickness skin defects of C57BL mice further demonstrated that SDF-1α expression and the number of peripheral EPCs at days 3, 5, and 7 in the SDF-1α ov HDF-mAM group were significantly higher than that in other groups ( p < 0.01). The local microvascular density at day 10 of transplantation showed that the microvascular density in the SDF-1α ov HDF-mAM group was significantly higher than that in HDF-mAM group ( p < 0.01). In conclusion, HDF-mAM had a strong proliferative activity and could be used to create a sound microenvironment for quick vascularization by secreting multiple cytokines and extracellular matrix components. Overexpression of SDF-1α could chemotaxize EPCs to reach local wounds, thus further accelerating angiogenesis in the transplant site. The technique described may prove to be a new model for accelerating vascularization of tissue and organ transplants and chronic ischemic wounds.
- Published
- 2016
- Full Text
- View/download PDF
5. Overexpression of Hypo-Phosphorylated IκBβ at Ser313 Protects the Heart against Sepsis.
- Author
-
Guang-Qing Wang, Tao Tang, Zhong-Shan Wang, Ying-Ying Liu, Li Wang, Peng-Fei Luo, and Zhao-Fan Xia
- Subjects
Medicine ,Science - Abstract
IκBβis an inhibitor of nuclear factor kappa B(NF-κB) and participates in the cardiac response to sepsis. However, the role of the hypo-phosphorylated form of IκBβ at Ser313, which can be detected during sepsis, is unknown. Here, we examined the effects of IκBβ with a mutation at Ser313→Ala313 on cardiac damage induced by sepsis. Transgenic (Tg) mice were generated to overexpress IκBβ, in which Ser-313 is replaced with alanine ubiquitously, in order to mimic the hypo-phosphorylated form of IκBβ. Survival analysis showed that Tg mice exhibited decreased inflammatory cytokine levels and decreased rates of mortality in comparison to wild type (WT) mice, after sepsis in a cecal-ligation and puncture model (CLP). Compared to WT septic mice, sepsis in Tg mice resulted in improved cardiac functions, lower levels of troponin I and decreased rates of cardiomyocyte apoptosis, compared to WT mice. The increased formation of autophagicvacuoles detected with electron microscopy demonstrated the enhancement of cardiac autophagy. This phenomenon was further confirmed by the differential expression of genes related to autophagy, such as LC3, Atg5, Beclin-1, and p62. The increased expression of Cathepsin L(Ctsl), a specific marker for mitochondrial stress response, may be associated with the beneficial effects of the hypo-phosphorylated form of IκBβ. Our observations suggest that the hypo-phosphorylated form of IκBβ at Ser313 is beneficial to the heart in sepsis through inhibition of apoptosisand enhancement of autophagy in mutated IκBβ transgenic mice.
- Published
- 2016
- Full Text
- View/download PDF
6. Epidemiological and Molecular Characteristics of the PB1-F2 Proteins in H7N9 Influenza Viruses, Jiangsu
- Author
-
Peng-fei Luo, Yang Xuan, Hairong Zi, Pingmin Wei, Yan Guo, Michael Cunningham, Taha Hussein Musa, and Wei Li
- Subjects
Adult ,Male ,Adolescent ,Article Subject ,viruses ,Molecular Sequence Data ,lcsh:Medicine ,Sequence alignment ,Biology ,medicine.disease_cause ,Influenza A Virus, H7N9 Subtype ,General Biochemistry, Genetics and Molecular Biology ,Virus ,Avian Influenza A Virus ,Cohort Studies ,Viral Proteins ,Young Adult ,Phylogenetics ,Influenza, Human ,Influenza A virus ,medicine ,Humans ,Amino Acid Sequence ,Selection, Genetic ,Phylogeny ,Aged ,Genetics ,Aged, 80 and over ,Molecular Epidemiology ,Models, Statistical ,General Immunology and Microbiology ,Molecular epidemiology ,Human evolutionary genetics ,Host (biology) ,lcsh:R ,General Medicine ,Middle Aged ,Virology ,Female ,Sequence Alignment ,Research Article - Abstract
The recent sporadic infections of humans in China with previously unrecognized avian influenza A virus of the H7N9 subtype (A(H7N9)) have caused concern. The aim is to find out the epidemiological and molecular analysis of the PB1-F2 proteins in H7N9 influenza viruses, in Jiangsu province. Sequences were obtained from GISAID database. Data were analyzed by using Molecular Evolutionary Genetics Analysis software and Bayesian Markov chain Monte Carlo method. From March 1, 2013, to May 31, 2014, 53 patients were confirmed to be infected with the H7N9 virus; one was a retrospective case in Jiangsu province. 38 sequences of PB1 in H7N9 of Jiangsu were obtained from the GISAID online and were then divided into three lineages. Of these sequences, 4 sequences and 3 sequences encode an N-terminally truncated PB1-F2 (52aa)polypeptide and C-terminally truncated PB1-F2 (76aa) polypeptide, respectively. The remaining sequences encode a full-length PB1-F2 (90aa). We estimated a mean evolutionary rate of 3.053 × 10−3 subs/site/year (95% HPD: 2.021 × 10−3–4.051 × 10−3). The site-by-site analysis of selection pressure analysis revealed positively and negatively (12, 3), respectively, selected sites. Influenza A (H7N9) virus adapting into new host, PB1-F2 of H7N9, might be faced with higher selection pressures.
- Published
- 2015
- Full Text
- View/download PDF
7. Vitamin D receptor genetic polymorphisms and tuberculosis among Chinese Han ethnic group
- Author
-
Shang, Cao, Peng-fei, Luo, Wei, Li, Wan-qin, Tang, Xiao-na, Cong, and Ping-min, Wei
- Subjects
Polymorphism, Genetic ,Asian People ,Case-Control Studies ,Humans ,Receptors, Calcitriol ,Tuberculosis ,Genetic Predisposition to Disease - Abstract
In epidemiological studies, tuberculosis (TB) appears intimately with vitamin D insufficiency whereas its relationship with vitamin D receptor (VDR) polymorphism caused by radical difference remains unspecified. This study aimed to investigate the relationship between vitamin D genetic polymorphism and tuberculosis in Han ethnic group.Meta-analysis was adopted in the synthetic quantitative analysis of documents home and abroad on the relationship between vitamin D genetic polymorphisms and tuberculosis, which were openly published during June 2000 to January 2010. Random effect model and fixed effect model analyses were used to calculate the incorporated odds ratio (OR) based on the heterogeneity test data.A total of 6 eligible studies were included in this analysis. The FokI-ff genotype showed a significant marginal association (Fixed effect model: OR 1.91, 95%CI 1.44-2.52; Random effect model: OR 1.91, 95%CI 0.94-3.88), yet TaqI polymorphisms was not significantly related to TB.The interaction between FoKI genotype polymorphism and TB observed demonstrates that vitamin D deficiency might exist as a risk factor during the development of TB in Han ethnic group and more evidences needed to validate the conclusion.
- Published
- 2012
8. [Research of high frequency induced thermotherapy efficiency for treatment of hepatocellular carcinoma]
- Author
-
Li-Gong, Lu, Bao-Shan, Hu, Yong, Li, Peng-Fei, Luo, and Xiao-Ming, Chen
- Subjects
Adult ,Male ,Carcinoma, Hepatocellular ,Adolescent ,Liver Neoplasms ,Hyperthermia, Induced ,Middle Aged ,Combined Modality Therapy ,Survival Analysis ,Young Adult ,Treatment Outcome ,Catheter Ablation ,Humans ,Female ,Chemoembolization, Therapeutic ,Aged ,Neoplasm Staging - Abstract
To investigate the effectiveness of high frequency induced thermotherapy in the treatment of hepatocellular carcinoma.We included a 65 proven cases of hepatocellular carcinoma in which the diagnosis was confirmed by pathology, radio-imaging and AFP value. The patients were randomly divided into 2 group by odd or even hospitalization number. A total of 32 patients were enrolled in the TACE + PEI only group and 33 patients were enrolled in the TACE + HITT therapy group.For the 32 patients in TACE + PEI only therapy group, the 1, 2, and 3 year of survival rates were 73%, 56%, and 23%, respectively, with a median survival of 1.876 years. For patients in the therapy group, the 1, 2, and 3 year survival rates were 87%, 76%, and 51%, respectively, with a median survival of 2.134 years. Survival rate and duration in the therapy group was significantly greater in the combination therapy group compared to the control group (P0.05). There was a negative correlation between the effectiveness of combination therapy and the mortality risk (P0.05).HiTT + TACE can significantly increase survival rate and extend length of survival in patients with hepatocellular carcinoma.
- Published
- 2008
9. [Transarterial oily chemoembolization combined with interstitial laser thermotherapy for treatment of hepatocellular carcinoma]
- Author
-
Ze-jian, Zhou, Rong-de, Xu, Wei-ke, Li, Wen-xing, Zhuang, Li-gong, Lu, Pei-jian, Shao, Xiao-ming, Chen, and Peng-fei, Luo
- Subjects
Survival Rate ,Carcinoma, Hepatocellular ,Treatment Outcome ,Lasers ,Liver Neoplasms ,Humans ,Hyperthermia, Induced ,Chemoembolization, Therapeutic - Abstract
To evaluate the therapeutic effects and adverse effects of transarterial oily chemoembolization combined with interstitial laser thermotherapy (TOCE+ILT) in the treatment of hepatocellular carcinoma.Totally 120 patients with hepatocellular carcinoma were randomized into two groups and received interventions with TOCE+ILT or TOCE combined with percutaneous ethanol injection (TOCE+PEI). The treatment was repeated when necessary until the tumor was completely ablated, after which the therapeutic effects were evaluated and the patients were the followed up for observing long-term clinical outcome.Of the 120 patients enrolled in this observation, 105 were followed up for two years (54 in TOCE+ILT group and 51 in TOCE+PEI group). The complete tumor necrosis rate of TOCE+ILT group was significantly higher than that of the TOCE+PEI group (84.8% vs 73.9%,Chi(2)=4.405, P=0.036), and TOCE+ILT was associated with a significantly higher negative conversion rate of AFP positivity (77.8% vs 56.1%, Chi(2)=4.592, P=0.032). The 1-year survival rate were similar between two groups, but the 2-year survival rate was significantly higher in patients with TOCE+ILT (79.6% vs 60.8%, Chi(2)=4.477, P=0.034). The hepatic function was comparable between the two groups before treatment, and 1 week after treatment, the ALT level in patients undergoing TOCE+ILT was significantly lower than that in patients with TOCE+PEI (95.90-/+56.06 U/L vs 116.31-/+45.27 U/L, t=2.04, P=0.043). Post-embolization syndrome was observed in the patients in two groups, but no severe adverse events were found.TOCE+ILT has good therapeutic effects and mild side effects in the treatment of hepatocellular carcinoma.
- Published
- 2007
10. Percutaneous transhepatic metal versus plastic biliary stent in treating malignant biliary obstruction: a multiple center investigation
- Author
-
Yuan-Xing, Guo, Yan-Hao, Li, Yong, Chen, Ping-Yan, Chen, Peng-Fei, Luo, Yong, Li, Hong, Shan, and Zai-Bo, Jiang
- Subjects
Adult ,Cholangiopancreatography, Endoscopic Retrograde ,Male ,Cholestasis ,Equipment Safety ,Palliative Care ,Equipment Design ,Middle Aged ,Magnetic Resonance Imaging ,Risk Assessment ,Catheterization ,Survival Rate ,Biliary Tract Neoplasms ,Treatment Outcome ,Metals ,Humans ,Female ,Stents ,Tomography, X-Ray Computed ,Plastics ,Aged ,Follow-Up Studies - Abstract
To compare the efficacy of metal versus plastic biliary stent implantation in the treatment of malignant biliary obstruction.Percutaneous transhepatic self-expandable metal stent (MS, n=61) and 10F plastic stent (PS, n=34) were placed in 95 patients with malignant biliary obstruction in three hospitals of Guangdong province. All patients were followed up until death or at least one year after the procedure. Kaplan-Meier analysis was used to compare the survival of the patients and the rates of stent patency.The 30-day mortality rate was lower in the MS group (6/61, 9.8%) than in the PS group (9/34, 26.5%, P0.05). The 30-day reobstruction rate and the complication rate were 15.0%, 16.4% in the MS group and 32.4%, 29.4% in the PS group, respectively (P0.01). The median patency period of stents and median survival period of the patients were 230 days, 224 days in the MS group and 90 days, 94 days in the PS group, respectively (P0.01).Metal stent is clinically superior to plastic stent in the treatment of malignant biliary obstruction.
- Published
- 2003
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.