15 results on '"Parrotta E"'
Search Results
2. In vitro CSC-derived cardiomyocytes exhibit the typical microRNA-mRNA blueprint of endogenous cardiomyocytes
- Author
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Teresa Mancuso, Francesco Rossi, Mariangela Scalise, Luca Salerno, Fabiola Marino, Annalaura Torella, Antonella De Angelis, Bernardo Nadal-Ginard, Marcello Rota, Donato Cappetta, Daniele Torella, Eleonora Cianflone, Alessandro Weisz, Elvira Immacolata Parrotta, Antonella Barone, Pierangelo Veltri, Liberato Berrino, Domenico Palumbo, Konrad Urbanek, Scalise, Mariangela, Marino, Fabiola, Salerno, Luca, Mancuso, Teresa, Cappetta, Donato, Barone, Antonella, Parrotta Elvira, Immacolata, Torella, Annalaura, Palumbo, Domenico, Veltri, Pierangelo, De Angelis, Antonella, Berrino, Liberato, Rossi, Francesco, Weisz, Alessandro, Rota, Marcello, Urbanek, Konrad, Nadal-Ginard, Bernardo, Torella, Daniele, Cianflone, Eleonora, Scalise, M., Marino, F., Salerno, L., Mancuso, T., Cappetta, D., Barone, A., Parrotta, E. I., Torella, A., Palumbo, D., Veltri, P., De Angelis, A., Berrino, L., Rossi, F., Weisz, A., Rota, M., Urbanek, K., Nadal-Ginard, B., Torella, D., and Cianflone, E.
- Subjects
QH301-705.5 ,Stem-cell differentiation ,Medicine (miscellaneous) ,Biology ,Muscle Development ,Article ,General Biochemistry, Genetics and Molecular Biology ,Transcriptome ,Mice ,Stem Cell ,microRNA ,medicine ,Animals ,Myocytes, Cardiac ,RNA, Messenger ,Biology (General) ,Progenitor cell ,reproductive and urinary physiology ,Messenger RNA ,Animal ,Stem Cells ,Cell Cycle ,Cardiac muscle ,MicroRNA ,Cell Differentiation ,Cell cycle ,Cell Cycle Gene ,Up-Regulation ,Cell biology ,MicroRNAs ,medicine.anatomical_structure ,embryonic structures ,Stem cell ,General Agricultural and Biological Sciences ,Heart stem cells - Abstract
miRNAs modulate cardiomyocyte specification by targeting mRNAs of cell cycle regulators and acting in cardiac muscle lineage gene regulatory loops. It is unknown if or to-what-extent these miRNA/mRNA networks are operative during cardiomyocyte differentiation of adult cardiac stem/progenitor cells (CSCs). Clonally-derived mouse CSCs differentiated into contracting cardiomyocytes in vitro (iCMs). Comparison of “CSCs vs. iCMs” mRNome and microRNome showed a balanced up-regulation of CM-related mRNAs together with a down-regulation of cell cycle and DNA replication mRNAs. The down-regulation of cell cycle genes and the up-regulation of the mature myofilament genes in iCMs reached intermediate levels between those of fetal and neonatal cardiomyocytes. Cardiomyo-miRs were up-regulated in iCMs. The specific networks of miRNA/mRNAs operative in iCMs closely resembled those of adult CMs (aCMs). miR-1 and miR-499 enhanced myogenic commitment toward terminal differentiation of iCMs. In conclusions, CSC specification/differentiation into contracting iCMs follows known cardiomyo-MiR-dependent developmental cardiomyocyte differentiation trajectories and iCMs transcriptome/miRNome resembles that of CMs., Scalise et al. examine the mRNAome and miRNAome of cardiomyocytes differentiated from murine adult cardiac stem cells (CSCs). Their results show that the differentiation process follows a trajectory of miRNA/mRNA expression that resembles that of adult cardiomyocytes.
- Published
- 2021
3. Statins Stimulate New Myocyte Formation After Myocardial Infarction by Activating Growth and Differentiation of the Endogenous Cardiac Stem Cells
- Author
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Mariangela Scalise, Bernardo Nadal-Ginard, Fabiola Marino, Liberato Berrino, Daniele Torella, Teresa Mancuso, Elvira Immacolata Parrotta, Francesco Rossi, Eleonora Cianflone, Donato Cappetta, Giovanni Cuda, Konrad Urbanek, Alessandro Salatino, Michele Albanese, Antonella De Angelis, Jolanda Sabatino, Cianflone, E., Cappetta, D., Mancuso, T., Sabatino, J., Marino, F., Scalise, M., Albanese, M., Salatino, A., Parrotta, E. I., Cuda, G., De Angelis, A., Berrino, L., Rossi, F., Nadal-Ginard, B., Torella, D., and Urbanek, K.
- Subjects
0301 basic medicine ,Simvastatin ,Myocardial Infarction ,030204 cardiovascular system & hematology ,Cardiac stem cell ,lcsh:Chemistry ,Mice ,0302 clinical medicine ,3-hydroxy-3-methylglutaryl coenzyme A ,Myocardial infarction ,Phosphorylation ,Rosuvastatin Calcium ,lcsh:QH301-705.5 ,Cells, Cultured ,Spectroscopy ,Pravastatin ,Cultured ,Akt ,Cardiac stem cells ,Myocardial regeneration ,Statins ,Animals ,Cell Differentiation ,Cell Proliferation ,Disease Models, Animal ,Female ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,Muscle Cells ,Myocardium ,Proto-Oncogene Proteins c-akt ,Rats ,Stem Cells ,cardiac stem cells ,General Medicine ,Computer Science Applications ,myocardial regeneration ,Stem cell ,medicine.drug ,Cells ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,medicine ,Rosuvastatin ,cardiovascular diseases ,Physical and Theoretical Chemistry ,Progenitor cell ,Molecular Biology ,Protein kinase B ,Animal ,business.industry ,Cell growth ,Organic Chemistry ,nutritional and metabolic diseases ,medicine.disease ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Disease Models ,Cancer research ,business - Abstract
The 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) exert pleiotropic effects on cardiac cell biology which are not yet fully understood. Here we tested whether statin treatment affects resident endogenous cardiac stem/progenitor cell (CSC) activation in vitro and in vivo after myocardial infarction (MI). Statins (Rosuvastatin, Simvastatin and Pravastatin) significantly increased CSC expansion in vitro as measured by both BrdU incorporation and cell growth curve. Additionally, statins increased CSC clonal expansion and cardiosphere formation. The effects of statins on CSC growth and differentiation depended on Akt phosphorylation. Twenty-eight days after myocardial infarction by permanent coronary ligation in rats, the number of endogenous CSCs in the infarct border zone was significantly increased by Rosuvastatin-treatment as compared to untreated controls. Additionally, commitment of the activated CSCs into the myogenic lineage (c-kitpos/Gata4pos CSCs) was increased by Rosuvastatin administration. Accordingly, Rosuvastatin fostered new cardiomyocyte formation after MI. Finally, Rosuvastatin treatment reversed the cardiomyogenic defects of CSCs in c-kit haploinsufficient mice, increasing new cardiomyocyte formation by endogenous CSCs in these mice after myocardial infarction. In summary, statins, by sustaining Akt activation, foster CSC growth and differentiation in vitro and in vivo. The activation and differentiation of the endogenous CSC pool and consequent new myocyte formation by statins improve myocardial remodeling after coronary occlusion in rodents. Similar effects might contribute to the beneficial effects of statins on human cardiovascular diseases.
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- 2020
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4. A passive microfluidic device for chemotaxis studies
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Maria Antonia D'Attimo, Ennio Carbone, Costanza Maria Cristiani, Enzo Di Fabrizio, Gerardo Perozziello, Ulrich Krühne, Giovanni Cuda, Francesco Guzzi, Elvira Immacolata Parrotta, Patrizio Candeloro, Elisabetta Dattola, Maria Laura Coluccio, E. Lamanna, Coluccio, M. L., D'Attimo, M. A., Cristiani, C. M., Candeloro, P., Parrotta, E., Dattola, E., Guzzi, F., Cuda, G., Lamanna, E., Carbone, E., Kruhne, U., Di Fabrizio, E., and Perozziello, G.
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Materials science ,Microscope ,Gravity force ,Diffusion ,lcsh:Mechanical engineering and machinery ,Microfluidics ,02 engineering and technology ,Mini incubator ,01 natural sciences ,Passive microfluidic device ,Article ,law.invention ,law ,lcsh:TJ1-1570 ,Electrical and Electronic Engineering ,chemotaxis ,business.industry ,Mechanical Engineering ,Chemotaxis ,010401 analytical chemistry ,Incubator ,Chemotaxi ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Volumetric flow rate ,Control and Systems Engineering ,Optoelectronics ,0210 nano-technology ,business ,Concentration gradient - Abstract
This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µ, L/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µ, m/min, comparable with the rates present in scientific literature.
- Published
- 2019
5. Microfluidics & nanotechnology: towards fully integrated analytical devices for the detection of cancer biomarkers
- Author
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Giovanni Cuda, E. Di Fabrizio, Patrizio Candeloro, Francesca Pardeo, Rossella Catalano, Horacio D. Espinosa, Andrea Adamo, Maria Laura Coluccio, Annalisa Nicastri, Elvira Immacolata Parrotta, Angela Mena Perri, Francesco Gentile, Gerardo Perozziello, Perozziello, G., Candeloro, P., Gentile, Francesco, Nicastri, A., Perri, A., Coluccio, M. L., Adamo, A., Pardeo, F., Catalano, R., Parrotta, E., Espinosa, H. D., Cuda, G., and Di Fabrizio, E.
- Subjects
Materials science ,Resolution (mass spectrometry) ,General Chemical Engineering ,Chemistry (all) ,Microfluidics ,Nanotechnology ,General Chemistry ,symbols.namesake ,Small peptide ,symbols ,Coming out ,Chemical Engineering (all) ,Raman spectroscopy ,Biosensor ,Hydrodynamic flow ,Raman scattering - Abstract
In this paper, we describe an innovative modular microfluidic platform allowing filtering, concentration and analysis of peptides from a complex mixture. The platform is composed of a microfluidic filtering device and a superhydrophobic surface integrating surface enhanced Raman scattering (SERS) sensors. The microfluidic device was used to filter specific peptides (MW 1553.73 D) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancers, from albumin (66.5 KD), the most represented protein in human plasma. The filtering process consisted of driving the complex mixture through a porous membrane having a cut-off of 12–14 kD by hydrodynamic flow. The filtered samples coming out of the microfluidic device were subsequently deposited on a superhydrophobic surface formed by micro pillars on top of which nanograins were fabricated. The nanograins coupled to a Raman spectroscopy instrument acted as a SERS sensor and allowed analysis of the filtered sample on top of the surface once it evaporated. By using the presented platform, we demonstrate being able to sort small peptides from bigger proteins and to detect them by using a label-free technique at a resolution down to 0.1 ng μL−1. The combination of microfluidics and nanotechnology to develop the presented microfluidic platform may give rise to a new generation of biosensors capable of detecting low concentration samples from complex mixtures without the need for any sample pretreatment or labelling. The developed devices could have future applications in the field of early diagnosis of severe illnesses, e.g. early cancer detection.
- Published
- 2014
6. Attention to cardiac sensations enhances the heartbeat-evoked potential during exhalation.
- Author
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Zaccaro A, Della Penna F, Mussini E, Parrotta E, Perrucci MG, Costantini M, and Ferri F
- Abstract
Respiration and cardiac activity intricately interact through complex physiological mechanisms. The heartbeat-evoked potential (HEP) is an EEG fluctuation reflecting the cortical processing of cardiac signals. We recently found higher HEP amplitude during exhalation than inhalation during a task involving attention to cardiac sensations. This may have been due to reduced cardiac perception during inhalation and heightened perception during exhalation through attentional mechanisms. To investigate relationships between HEP, attention, and respiration, we introduced an experimental setup that included tasks related to cardiac and respiratory interoceptive and exteroceptive attention. Results revealed HEP amplitude increases during the interoceptive tasks over fronto-central electrodes. When respiratory phases were taken into account, HEP increases were primarily driven by heartbeats recorded during exhalation, specifically during the cardiac interoceptive task, while inhalation had minimal impact. These findings emphasize the role of respiration in cardiac interoceptive attention and could have implications for respiratory interventions to fine-tune cardiac interoception., Competing Interests: The authors declare no competing interests., (© 2024 The Author(s).)
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- 2024
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7. Brain-heart interactions are modulated across the respiratory cycle via interoceptive attention.
- Author
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Zaccaro A, Perrucci MG, Parrotta E, Costantini M, and Ferri F
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- Attention physiology, Awareness physiology, Brain physiology, Heart Rate physiology, Humans, Respiratory Rate, Electroencephalography methods, Interoception physiology
- Abstract
Respiration and heartbeat continuously interact within the living organism at many different levels, representing two of the main oscillatory rhythms of the body and providing major sources of interoceptive information to the brain. Despite the modulatory effect of respiration on exteroception and cognition has been recently established in humans, its role in shaping interoceptive perception has been scarcely investigated so far. In two independent studies, we investigated the effect of spontaneous breathing on cardiac interoception by assessing the Heartbeat Evoked Potential (HEP) in healthy humans. In Study 1, we compared HEP activity for heartbeats occurred during inhalation and exhalation in 40 volunteers at rest. We found higher HEP amplitude during exhalation, compared to inhalation, over fronto-centro-parietal areas. This suggests increased brain-heart interactions and improved cortical processing of the heartbeats during exhalation. Further analyses revealed that this effect was moderated by heart rate changes. In Study 2, we tested the respiratory phase-dependent modulation of HEP activity in 20 volunteers during Exteroceptive and Interoceptive conditions of the Heartbeat Detection (HBD) task. In these conditions, participants were requested to tap at each heartbeat, either listened to or felt, respectively. Results showed higher HEP activity and higher detection accuracy at exhalation than inhalation in the Interoceptive condition only. Direct comparisons of Interoceptive and Exteroceptive conditions confirmed stronger respiratory phase-dependent modulation of HEP and accuracy when attention was directed towards the interoceptive stimuli. Moreover, HEP changes during the Interoceptive condition were independent of heart physiology, but were positively correlated with higher detection accuracy at exhalation than inhalation. This suggests a link between optimization of cortical processing of cardiac signals and detection of heartbeats across the respiratory cycle. Overall, we provide data showing that respiration shapes cardiac interoception at the neurophysiological and behavioural levels. Specifically, exhalation may allow attentional shift towards the internal bodily states., Competing Interests: Declaration of Competing Interest The authors declare no competing financial interest., (Copyright © 2022. Published by Elsevier Inc.)
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- 2022
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8. Microfluidics for 3D Cell and Tissue Cultures: Microfabricative and Ethical Aspects Updates.
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Limongi T, Guzzi F, Parrotta E, Candeloro P, Scalise S, Lucchino V, Gentile F, Tirinato L, Coluccio ML, Torre B, Allione M, Marini M, Susa F, Fabrizio ED, Cuda G, and Perozziello G
- Subjects
- Animals, Biocompatible Materials, Cell Culture Techniques methods, Lab-On-A-Chip Devices, Microfluidics methods
- Abstract
The necessity to improve in vitro cell screening assays is becoming ever more important. Pharmaceutical companies, research laboratories and hospitals require technologies that help to speed up conventional screening and therapeutic procedures to produce more data in a short time in a realistic and reliable manner. The design of new solutions for test biomaterials and active molecules is one of the urgent problems of preclinical screening and the limited correlation between in vitro and in vivo data remains one of the major issues. The establishment of the most suitable in vitro model provides reduction in times, costs and, last but not least, in the number of animal experiments as recommended by the 3Rs (replace, reduce, refine) ethical guiding principles for testing involving animals. Although two-dimensional (2D) traditional cell screening assays are generally cheap and practical to manage, they have strong limitations, as cells, within the transition from the three-dimensional (3D) in vivo to the 2D in vitro growth conditions, do not properly mimic the real morphologies and physiology of their native tissues. In the study of human pathologies, especially, animal experiments provide data closer to what happens in the target organ or apparatus, but they imply slow and costly procedures and they generally do not fully accomplish the 3Rs recommendations, i.e., the amount of laboratory animals and the stress that they undergo must be minimized. Microfluidic devices seem to offer different advantages in relation to the mentioned issues. This review aims to describe the critical issues connected with the conventional cells culture and screening procedures, showing what happens in the in vivo physiological micro and nano environment also from a physical point of view. During the discussion, some microfluidic tools and their components are described to explain how these devices can circumvent the actual limitations described in the introduction.
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- 2022
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9. The Expanding Clinical Spectrum of Myelin Oligodendrocyte Glycoprotein (MOG) Antibody Associated Disease in Children and Adults.
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Parrotta E and Kister I
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- 2020
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10. COVID-19 outcomes in MS: Observational study of early experience from NYU Multiple Sclerosis Comprehensive Care Center.
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Parrotta E, Kister I, Charvet L, Sammarco C, Saha V, Charlson RE, Howard J, Gutman JM, Gottesman M, Abou-Fayssal N, Wolintz R, Keilson M, Fernandez-Carbonell C, Krupp LB, and Zhovtis Ryerson L
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- Adolescent, Adult, Aged, Antiviral Agents adverse effects, Antiviral Agents therapeutic use, COVID-19, Coronavirus Infections complications, Female, Hospitalization, Humans, Hydroxychloroquine adverse effects, Hydroxychloroquine therapeutic use, Male, Middle Aged, Pandemics, Pneumonia, Viral complications, Risk Factors, SARS-CoV-2, Time Factors, Young Adult, COVID-19 Drug Treatment, Betacoronavirus drug effects, Coronavirus Infections drug therapy, Multiple Sclerosis complications, Pneumonia, Viral drug therapy
- Abstract
Objective: To report outcomes on patients with multiple sclerosis (MS) and related disorders with coronavirus disease 2019 (COVID-19) illness., Methods: From March 16 to April 30, 2020, patients with MS or related disorders at NYU Langone MS Comprehensive Care Center were identified with laboratory-confirmed or suspected COVID-19. The diagnosis was established using a standardized questionnaire or by review of in-patient hospital records., Results: We identified 76 patients (55 with relapsing MS, of which 9 had pediatric onset; 17 with progressive MS; and 4 with related disorders). Thirty-seven underwent PCR testing and were confirmed positive. Of the entire group, 64 (84%) patients were on disease-modifying therapy (DMT) including anti-CD20 therapies (n = 34, 44.7%) and sphingosine-1-phosphate receptor modulators (n = 10, 13.5%). The most common COVID-19 symptoms were fever and cough, but 21.1% of patients had neurologic symptom recrudescence preceding or coinciding with the infection. A total of 18 (23.7%) were hospitalized; 8 (10.5%) had COVID-19 critical illness or related death. Features more common among those hospitalized or with critical illness or death were older age, presence of comorbidities, progressive disease, and a nonambulatory status. No DMT class was associated with an increased risk of hospitalization or fatal outcome., Conclusions: Most patients with MS with COVID-19 do not require hospitalization despite being on DMTs. Factors associated with critical illness were similar to the general at-risk patient population. DMT use did not emerge as a predictor of poor COVID-19 outcome in this preliminary sample., (Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.)
- Published
- 2020
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11. Role of the occipito-temporal theta rhythm in hand visual identification.
- Author
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Moreau Q, Parrotta E, Era V, Martelli ML, and Candidi M
- Subjects
- Adult, Female, Humans, Male, Young Adult, Evoked Potentials physiology, Hand, Occipital Lobe physiology, Pattern Recognition, Visual physiology, Temporal Lobe physiology, Theta Rhythm physiology, Visual Perception physiology
- Abstract
Neuroimaging and EEG studies have shown that passive observation of the full body and of specific body parts is associated with 1 ) activity of an occipito-temporal region named the extrastriate body area (EBA), 2 ) amplitude modulations of a specific posterior event-related potential (ERP) component (N1/N190), and 3 ) a theta-band (4-7 Hz) synchronization recorded from occipito-temporal electrodes compatible with the location of EBA. To characterize the functional role of the occipito-temporal theta-band increase during the processing of body-part stimuli, we recorded EEG from healthy participants while they were engaged in an identification task (match-to-sample) of images of hands and nonbody control images (leaves). In addition to confirming that occipito-temporal electrodes show a larger N1 for hand images compared with control stimuli, cluster-based analysis revealed an occipito-temporal cluster showing an increased theta power when hands are presented (compared with leaves) and show that this theta increase is higher for identified hands compared with nonidentified ones while not being significantly different between not identified nonhand stimuli. Finally, single trial multivariate pattern analysis revealed that time-frequency modulation in the theta band is a better marker for classifying the identification of hand images than the ERP modulation. The present results support the notion that theta activity over the occipito-temporal cortex is an informative marker of hand visual processing and may reflect the activity of a network coding for stimulus identity. NEW & NOTEWORTHY Hands provide crucial information regarding the identity of others, which is a key information for social processes. We recorded EEG activity of healthy participants during the visual identification of hand images. The combination of univariate and multivariate pattern analysis in time- and time-frequency domain highlights the functional role of theta (4-7 Hz) activity over visual areas during hand identification and emphasizes the robustness of this neuromarker in occipito-temporal visual processing dynamics.
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- 2020
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12. A Passive Microfluidic Device for Chemotaxis Studies.
- Author
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Coluccio ML, D'Attimo MA, Cristiani CM, Candeloro P, Parrotta E, Dattola E, Guzzi F, Cuda G, Lamanna E, Carbone E, Krühne U, Di Fabrizio E, and Perozziello G
- Abstract
This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO
2 . Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.- Published
- 2019
- Full Text
- View/download PDF
13. Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy.
- Author
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Parrotta E, De Angelis MT, Scalise S, Candeloro P, Santamaria G, Paonessa M, Coluccio ML, Perozziello G, De Vitis S, Sgura A, Coluzzi E, Mollace V, Di Fabrizio EM, and Cuda G
- Subjects
- Biomarkers metabolism, Cell Cycle genetics, Cell Differentiation, Cluster Analysis, DNA metabolism, Fibroblasts cytology, Fibroblasts metabolism, Gene Expression, Gene Expression Profiling, Genetic Vectors chemistry, Genetic Vectors metabolism, Human Embryonic Stem Cells cytology, Humans, Induced Pluripotent Stem Cells cytology, Karyotyping, Kruppel-Like Factor 4, Kruppel-Like Transcription Factors genetics, Kruppel-Like Transcription Factors metabolism, Octamer Transcription Factor-3 genetics, Octamer Transcription Factor-3 metabolism, Primary Cell Culture, Principal Component Analysis, Proto-Oncogene Proteins c-myc genetics, Proto-Oncogene Proteins c-myc metabolism, RNA metabolism, SOXB1 Transcription Factors genetics, SOXB1 Transcription Factors metabolism, Sendai virus genetics, Sendai virus metabolism, Transfection, DNA genetics, Human Embryonic Stem Cells metabolism, Induced Pluripotent Stem Cells metabolism, RNA genetics, Spectrum Analysis, Raman
- Abstract
Background: Human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells, hold enormous promise for many biomedical applications, such as regenerative medicine, drug testing, and disease modeling. Although induced pluripotent stem cells resemble embryonic stem cells both morphologically and functionally, the extent to which these cell lines are truly equivalent, from a molecular point of view, remains controversial., Methods: Principal component analysis and K-means cluster analysis of collected Raman spectroscopy data were used for a comparative study of the biochemical fingerprint of human induced pluripotent stem cells and human embryonic stem cells. The Raman spectra analysis results were further validated by conventional biological assays., Results: Raman spectra analysis revealed that the major difference between human embryonic stem cells and induced pluripotent stem cells is due to the nucleic acid content, as shown by the strong positive peaks at 785, 1098, 1334, 1371, 1484, and 1575 cm
-1 , which is enriched in human induced pluripotent stem cells., Conclusions: Here, we report a nonbiological approach to discriminate human induced pluripotent stem cells from their native embryonic stem cell counterparts.- Published
- 2017
- Full Text
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14. Antisense-mediated exon skipping: a therapeutic strategy for titin-based dilated cardiomyopathy.
- Author
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Gramlich M, Pane LS, Zhou Q, Chen Z, Murgia M, Schötterl S, Goedel A, Metzger K, Brade T, Parrotta E, Schaller M, Gerull B, Thierfelder L, Aartsma-Rus A, Labeit S, Atherton JJ, McGaughran J, Harvey RP, Sinnecker D, Mann M, Laugwitz KL, Gawaz MP, and Moretti A
- Subjects
- Animals, Cardiomyopathy, Dilated therapy, Cells, Cultured, Connectin genetics, Cytological Techniques, Disease Models, Animal, Genetic Therapy methods, Humans, Mice, Mice, Transgenic, Myocytes, Cardiac drug effects, Myocytes, Cardiac physiology, Myofibrils metabolism, Myofibrils physiology, Oligonucleotides, Antisense genetics, Oligonucleotides, Antisense therapeutic use, Cardiomyopathy, Dilated physiopathology, Connectin metabolism, Exons, Frameshift Mutation, Gene Expression Regulation drug effects, Oligonucleotides, Antisense metabolism
- Abstract
Frameshift mutations in the TTN gene encoding titin are a major cause for inherited forms of dilated cardiomyopathy (DCM), a heart disease characterized by ventricular dilatation, systolic dysfunction, and progressive heart failure. To date, there are no specific treatment options for DCM patients but heart transplantation. Here, we show the beneficial potential of reframing titin transcripts by antisense oligonucleotide (AON)-mediated exon skipping in human and murine models of DCM carrying a previously identified autosomal-dominant frameshift mutation in titin exon 326. Correction of TTN reading frame in patient-specific cardiomyocytes derived from induced pluripotent stem cells rescued defective myofibril assembly and stability and normalized the sarcomeric protein expression. AON treatment in Ttn knock-in mice improved sarcomere formation and contractile performance in homozygous embryos and prevented the development of the DCM phenotype in heterozygous animals. These results demonstrate that disruption of the titin reading frame due to a truncating DCM mutation can be restored by exon skipping in both patient cardiomyocytes in vitro and mouse heart in vivo, indicating RNA-based strategies as a potential treatment option for DCM., (© 2015 The Authors. Published under the terms of the CC BY 4.0 license.)
- Published
- 2015
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15. Functional comparison of induced pluripotent stem cell- and blood-derived GPIIbIIIa deficient platelets.
- Author
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Orban M, Goedel A, Haas J, Sandrock-Lang K, Gärtner F, Jung CB, Zieger B, Parrotta E, Kurnik K, Sinnecker D, Wanner G, Laugwitz KL, Massberg S, and Moretti A
- Subjects
- Adolescent, Cell Adhesion, Cells, Cultured, Female, Fibrinogen metabolism, Humans, Induced Pluripotent Stem Cells metabolism, Integrin alpha2 genetics, Integrin alpha2 metabolism, Models, Biological, Thrombasthenia genetics, Blood Platelets cytology, Blood Platelets metabolism, Induced Pluripotent Stem Cells cytology, Thrombasthenia pathology
- Abstract
Human induced pluripotent stem cells (hiPSCs) represent a versatile tool to model genetic diseases and are a potential source for cell transfusion therapies. However, it remains elusive to which extent patient-specific hiPSC-derived cells functionally resemble their native counterparts. Here, we generated a hiPSC model of the primary platelet disease Glanzmann thrombasthenia (GT), characterized by dysfunction of the integrin receptor GPIIbIIIa, and compared side-by-side healthy and diseased hiPSC-derived platelets with peripheral blood platelets. Both GT-hiPSC-derived platelets and their peripheral blood equivalents showed absence of membrane expression of GPIIbIIIa, a reduction of PAC-1 binding, surface spreading and adherence to fibrinogen. We demonstrated that GT-hiPSC-derived platelets recapitulate molecular and functional aspects of the disease and show comparable behavior to their native counterparts encouraging the further use of hiPSC-based disease models as well as the transition towards a clinical application.
- Published
- 2015
- Full Text
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