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2. Fine-tuning modulation of oxidation-mediated posttranslational control of Bradyrhizobium diazoefficiens FixK2 transcription factor

Author

Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Ministerio de Educación, Cultura y Deporte (España), Biotechnology and Biological Sciences Research Council (UK), Parejo, Sergio, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Bedmar, Eulogio J., Gates, Andrew J., Mesa, Socorro, Ministerio de Ciencia e Innovación (España), Junta de Andalucía, Ministerio de Educación, Cultura y Deporte (España), Biotechnology and Biological Sciences Research Council (UK), Parejo, Sergio, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Bedmar, Eulogio J., Gates, Andrew J., and Mesa, Socorro

Published
2022

3. Distinctive phehotypes of Bradyrhizobium diazoefficiens transcriptional regulator (phaR) mutants grown on different carbon sources.

Author

Díaz-Peña, R., Cabrera Rodríguez, Juan José, Nashmías, M.E., Zubimendi, J.P., Parejo, Sergio, Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, Mongiardini, E.J., Müller-Santos, M., Pettinari, M.J., Mesa, Socorro, Díaz-Peña, R., Cabrera Rodríguez, Juan José, Nashmías, M.E., Zubimendi, J.P., Parejo, Sergio, Tortosa Muñoz, Germán, Delgado Igeño, María Jesús, Mongiardini, E.J., Müller-Santos, M., Pettinari, M.J., and Mesa, Socorro

Published
2022

4. Fine-Tuning Modulation of Oxidation-Mediated Posttranslational Control of Bradyrhizobium diazoefficiens FixK2 Transcription Factor

Author

Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Parejo, Sergio, Cabrera, Juan J., Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Bedmar, Eulogio J., Gates, Andrew J., Mesa, Socorro, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Junta de Andalucía, Parejo, Sergio, Cabrera, Juan J., Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Bedmar, Eulogio J., Gates, Andrew J., and Mesa, Socorro

Abstract

FixK2 is a CRP/FNR-type transcription factor that plays a central role in a sophisticated regulatory network for the anoxic, microoxic and symbiotic lifestyles of the soybean endosymbiont Bradyrhizobium diazoefficiens. Aside from the balanced expression of the fixK2 gene under microoxic conditions (induced by the two-component regulatory system FixLJ and negatively auto-repressed), FixK2 activity is posttranslationally controlled by proteolysis, and by the oxidation of a singular cysteine residue (C183) near its DNA-binding domain. To simulate the permanent oxidation of FixK2, we replaced C183 for aspartic acid. Purified C183D FixK2 protein showed both low DNA binding and in vitro transcriptional activation from the promoter of the fixNOQP operon, required for respiration under symbiosis. However, in a B. diazoefficiens strain coding for C183D FixK2, expression of a fixNOQP’-‘lacZ fusion was similar to that in the wild type, when both strains were grown microoxically. The C183D FixK2 encoding strain also showed a wild-type phenotype in symbiosis with soybeans, and increased fixK2 gene expression levels and FixK2 protein abundance in cells. These two latter observations, together with the global transcriptional profile of the microoxically cultured C183D FixK2 encoding strain, suggest the existence of a finely tuned regulatory strategy to counterbalance the oxidation-mediated inactivation of FixK2 in vivo.

Published
2022

5. Determinantes moleculares de la interacción FixK2-ADN implicados en la adaptación de Bradyrhizobium diazoefficiens a sus diferentes modus viven

Author

Ministerio de Economía y Competitividad (España), European Commission, Junta de Andalucía, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Parejo, Sergio, Juárez, Raquel A., Casado, Sara, Torres Porras, María Jesús, Bedmar, Eulogio J., Delgado Igeño, María Jesús, Mesa, Socorro, Ministerio de Economía y Competitividad (España), European Commission, Junta de Andalucía, Cabrera Rodríguez, Juan José, Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Parejo, Sergio, Juárez, Raquel A., Casado, Sara, Torres Porras, María Jesús, Bedmar, Eulogio J., Delgado Igeño, María Jesús, and Mesa, Socorro

Published
2022

6. Fine-tuning modulation of oxidation-mediated posttranslational control of Bradyrhizobium diazoefficiens FixK2 transcription factor

Author

European Commission, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Ministerio de Educación, Cultura y Deporte (España), Parejo, Sergio, Cabrera, Juan J., Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Gates, Andrew J., Bedmar, Eulogio J., Mesa, Socorro, European Commission, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Ministerio de Educación, Cultura y Deporte (España), Parejo, Sergio, Cabrera, Juan J., Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Gates, Andrew J., Bedmar, Eulogio J., and Mesa, Socorro

Published
2021

7. Oxidative stress produced by paraquat reduces nitrogen fixation in soybean-Bradyrhizobium diazoefficiens symbiosis by decreasing nodule functionality

Author

European Commission, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Tortosa Muñoz, Germán, Parejo, Sergio, Cabrera, Juan J., Bedmar, Eulogio J., Mesa, Socorro, European Commission, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Tortosa Muñoz, Germán, Parejo, Sergio, Cabrera, Juan J., Bedmar, Eulogio J., and Mesa, Socorro

Abstract

Soybean (Glycine max.) is one of the most important legumes cultivated worldwide. Its productivity can be altered by some biotic and abiotic stresses like global warming, soil metal pollution or over-application of herbicides like paraquat (1,1’-dimethyl-4,4’-bipyridinium dichloride). In this study, the effect of oxidative stress produced by paraquat addition (0, 20, 50 and 100 µM) during plant growth on symbiotic nitrogen fixation (SNF) and functionality of Bradyrhizobium diazoefficiens-elicited soybean nodules were evaluated. Results showed that the 50 µM was the threshold that B. diazoefficiens can tolerate under free-living conditions. In symbiosis with soybean, the paraquat addition statistically reduced the shoot and root dry weight of soybean plants, and number and development of the nodules. SNF was negatively affected by paraquat, which reduced total nitrogen content and fixed nitrogen close to 50% when 100 µM was added. These effects were due to the impairment of nodule functionality and the increased oxidative status of the nodules, as revealed by the lower leghaemoglobin content and the higher lipid peroxidation in soybean nodules from paraquat-treated plants.

Published
2021

8. Dissection of fixk2 protein-DNA interaction unveils new insights into Bradyrhizobium diazoefficiens lifestyles control

Author

European Commission, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Cabrera, Juan J., Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Parejo, Sergio, Casado, Sara, Torres Porras, María Jesús, Bedmar, Eulogio J., Delgado Igeño, María Jesús, Mesa, Socorro, European Commission, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Cabrera, Juan J., Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Parejo, Sergio, Casado, Sara, Torres Porras, María Jesús, Bedmar, Eulogio J., Delgado Igeño, María Jesús, and Mesa, Socorro

Published
2021

9. Fine-Tuning Modulation of Oxidation-Mediated Posttranslational Control of Bradyrhizobium diazoefficiens FixK 2 Transcription Factor.

Author

Parejo, Sergio, Cabrera, Juan J., Jiménez-Leiva, Andrea, Tomás-Gallardo, Laura, Bedmar, Eulogio J., Gates, Andrew J., and Mesa, Socorro

Subjects
*TRANSCRIPTION factors, *BRADYRHIZOBIUM, *ASPARTIC acid, *GENE expression, *DNA-protein interactions
Abstract

FixK2 is a CRP/FNR-type transcription factor that plays a central role in a sophisticated regulatory network for the anoxic, microoxic and symbiotic lifestyles of the soybean endosymbiont Bradyrhizobium diazoefficiens. Aside from the balanced expression of the fixK2 gene under microoxic conditions (induced by the two-component regulatory system FixLJ and negatively auto-repressed), FixK2 activity is posttranslationally controlled by proteolysis, and by the oxidation of a singular cysteine residue (C183) near its DNA-binding domain. To simulate the permanent oxidation of FixK2, we replaced C183 for aspartic acid. Purified C183D FixK2 protein showed both low DNA binding and in vitro transcriptional activation from the promoter of the fixNOQP operon, required for respiration under symbiosis. However, in a B. diazoefficiens strain coding for C183D FixK2, expression of a fixNOQP'-'lacZ fusion was similar to that in the wild type, when both strains were grown microoxically. The C183D FixK2 encoding strain also showed a wild-type phenotype in symbiosis with soybeans, and increased fixK2 gene expression levels and FixK2 protein abundance in cells. These two latter observations, together with the global transcriptional profile of the microoxically cultured C183D FixK2 encoding strain, suggest the existence of a finely tuned regulatory strategy to counterbalance the oxidation-mediated inactivation of FixK2 in vivo. [ABSTRACT FROM AUTHOR]

Published
2022
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